A PVDF membrane transfer tank

Abstract

The utility model discloses a kind of membrane transfer tank of PVDF film, including tank body, its inside is provided with cathode tube and anode tube, the top of tank body is set as opening, clamping plate is set in the top of tank body, the bottom of clamping plate is fixedly connected with several groups of fixed plate, clamping plate is located in the side of several groups of fixed plate respectively is provided with moving plate, the top of clamping plate is provided with the adjusting assembly for moving moving plate to this PVDF film is clamped and released, adjusting assembly includes mounting plate, screw rod, hand wheel and movable block, by setting several groups of fixed plate in the bottom of clamping plate, and moving plate is correspondingly set in the side of fixed plate, cooperate the adjusting assembly for moving moving plate in top, in the process of transfer printing experiment, multiple PVDF films can be quickly clamped and released operation, operation mode is simple and intuitive, experimental personnel does not need complex operation skill and a large amount of time, can easily complete the clamping and fixing of multiple PVDF films, greatly improve the convenience and efficiency of experimental operation.

Description

Technical Field

[0001] This utility model relates to the field of biotechnology experimental equipment technology, specifically a PVDF membrane transfer tank. Background Technology

[0002] As the main executors of life activities, proteins are crucial for understanding the physiological and pathological processes of organisms, and information such as their expression levels, modification states, and interactions is essential. PVDF membranes have been widely used in the field of protein transfer due to their unique properties.

[0003] When multiple PVDF films need to be transferred, the operation of existing transfer tank clamps is cumbersome. Specifically, the clamps are usually composed of multiple parts with a complex structure. Experimenters need to spend a lot of time and effort to assemble them, which reduces experimental efficiency. Therefore, we need to propose a PVDF film transfer tank. Utility Model Content

[0004] The purpose of this invention is to provide a PVDF membrane transfer groove that, through the cooperation of the bottom fixed plate, the moving plate and the top adjustment component of the clamping plate, can quickly and easily clamp and release multiple sets of PVDF membranes, greatly improving the convenience and efficiency of experimental operations, thereby solving the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution:

[0006] A PVDF membrane transfer tank includes: a tank body, in which a cathode tube and an anode tube are disposed inside, and the top of the tank body is open;

[0007] The clamping plate is located at the top of the tank. Several sets of fixed plates are fixedly connected to the bottom of the clamping plate. Movable plates are respectively set on one side of the clamping plate and the top of the clamping plate is equipped with an adjustment component for moving the movable plates to clamp and release the PVDF membrane.

[0008] Preferably, the adjustment assembly includes a mounting plate, with two sets of mounting plates fixedly mounted on the top of the clamping plate. A lead screw is rotatably mounted on the opposite side of the mounting plate. One end of the lead screw passes through one of the mounting plates and is fixedly connected to a handwheel. Several sets of movable blocks are threaded onto the outer wall of the lead screw. The bottom of the movable blocks passes through the clamping plate and is fixedly connected to the top of the movable plate.

[0009] Preferably, the top of the clamping plate is provided with several sets of limiting slots that are adapted to the movable block, and the movable block is slidably connected inside the limiting slots.

[0010] Preferably, the top of the groove is provided with several sets of slots, and the bottom of the clamping plate is fixedly connected with several sets of blocks that are adapted to the slots, and the blocks are engaged inside the slots.

[0011] Preferably, the top of the tank is provided with a positioning component for quick assembly and disassembly of the cathode tube and the anode tube. The positioning component includes a positioning tube, which is fixedly embedded in the top of the tank. The cathode tube and the anode tube are respectively inserted into the inside of the positioning tube. Fixing rings are fixedly sleeved on the outer walls of the cathode tube and the anode tube, and the fixing rings are threaded to the outer walls of the positioning tube.

[0012] Preferably, an installation groove is provided on one side wall of the tank, and a cooling component is provided inside the installation groove to cool the transfer buffer inside the tank to prevent protein denaturation.

[0013] Preferably, the cooling component includes a thermoelectric cooler, which is fixedly installed on one side inner wall of the tank. A heat dissipation fin is fixedly connected to one side wall of the thermoelectric cooler, and the heat dissipation fin is fixedly embedded in the inside of the mounting groove, with one end of the heat dissipation fin penetrating through the mounting groove.

[0014] Compared with the prior art, the beneficial effects of this utility model are:

[0015] This invention features several fixed plates at the bottom of the clamping plate and a movable plate on one side of each fixed plate. Combined with an adjustment component at the top for moving the movable plate, multiple PVDF films can be quickly clamped and released during the transfer experiment. The operation is simple and intuitive, and experimenters can easily clamp and fix multiple PVDF films without complex operating skills or a lot of time, greatly improving the convenience and efficiency of the experimental operation. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the structure of this utility model;

[0017] Figure 2 This is a schematic diagram of the structure of the groove and clamping plate of this utility model;

[0018] Figure 3 This is a schematic diagram of the structure of the fixed plate, the moving plate, and the adjustment assembly of this utility model;

[0019] Figure 4 This is a schematic diagram of the structure of the tank and cooling components of this utility model.

[0020] In the diagram: 1. Tank; 2. Cathode tube; 3. Anode tube; 4. Clamping plate; 5. Fixed plate; 6. Moving plate; 7. Adjustment assembly; 701. Mounting plate; 702. Lead screw; 703. Handwheel; 704. Moving block; 8. Limiting slot; 9. Slot; 10. Locking block; 11. Positioning assembly; 1101. Positioning tube; 1102. Fixing ring; 12. Cooling assembly; 1201. Semiconductor cooling chip; 1202. Heat dissipation fins; 13. Mounting slot. Detailed Implementation

[0021] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0022] Please see Figure 1-4 This utility model provides a technical solution:

[0023] A PVDF membrane transfer tank includes: a tank body 1, which serves as the basic support structure of the entire transfer tank and provides the necessary space for the transfer reaction. A cathode tube 2 and an anode tube 3 are installed inside the tank body 1. The top of the tank body 1 is open. The cathode tube 2 and the anode tube 3 are key components in the transfer process. They are connected to the positive and negative terminals of the power supply, respectively. By forming an electric field inside the tank body 1, charged protein molecules are driven to migrate from the gel to the PVDF membrane. The open design at the top of the tank body 1 makes it convenient for the experimenter to put in or take out the clamping plate 4 containing the PVDF membrane and the gel.

[0024] The clamping plate 4 is located at the top of the tank 1. Several sets of fixed plates 5 are fixedly connected to the bottom of the clamping plate 4. Movable plates 6 are respectively set on one side of the clamping plate 4 and the top of the clamping plate 4 is equipped with an adjustment component 7 for moving the movable plates 6 to clamp and release the PVDF membrane. The main function of the clamping plate 4 is to fix the PVDF membrane and gel, ensuring that they remain in close contact during the membrane transfer process. The fixed plates 5 provide a fixed support surface for the PVDF membrane and gel. The movable plates 6 clamp and release the PVDF membrane by being driven by the adjustment component 7. This design allows the experimenter to perform independent clamping operations on multiple sets of PVDF membranes according to actual needs, improving the flexibility and accuracy of the operation.

[0025] The adjusting assembly 7 includes mounting plates 701, with two sets of mounting plates 701 fixedly mounted on the top of the clamping plate 4. A lead screw 702 is rotatably mounted on the opposite side of the mounting plates 701. One end of the lead screw 702 passes through one set of mounting plates 701 and is fixedly connected to a handwheel 703. Several sets of movable blocks 704 are threaded onto the outer wall of the lead screw 702. The bottom of each movable block 704 passes through the clamping plate 4 and is fixedly connected to the top of the movable plate 6. The mounting plates 701 provide a stable mounting base for the lead screw 702. The lead screw 702 rotates under the drive of the handwheel 703. The movable block 704 is threadedly connected to the lead screw 702, and the bottom of the movable block 704 is fixedly connected to the moving plate 6. Therefore, the rotation of the lead screw 702 will drive the movable block 704 to move along the axial direction of the lead screw 702, thereby realizing the movement of the moving plate 6. This lead screw transmission method has the advantages of high transmission accuracy and labor-saving operation. By setting a reasonable structure of mounting plate 701, lead screw 702, handwheel 703 and movable block 704, the effect of accurately controlling the moving distance of the moving plate 6 can be achieved, which makes it convenient for experimental personnel to make flexible adjustments according to different specifications of PVDF membranes and gels.

[0026] The top of the clamping plate 4 is provided with several sets of limiting slots 8 that are adapted to the movable block 704. The movable block 704 is slidably connected inside the limiting slots 8. The limiting slots 8 provide precise guidance for the movement of the movable block 704 and restrict the direction of movement of the movable block 704, so that it can only move along the length direction of the limiting slots 8. This can prevent the movable block 704 from deviating or shaking during movement, and ensure the stability and accuracy of the movement of the moving plate 6. By setting the limiting slots 8, the effect of ensuring the precise movement trajectory of the movable block 704 is achieved, which further improves the stability and reliability of the clamping plate 4 for the PVDF film.

[0027] The top of the tank 1 has several sets of slots 9, and the bottom of the clamping plate 4 is fixedly connected to several sets of locking blocks 10 that are adapted to the slots 9. The locking blocks 10 are engaged inside the slots 9. The engaging structure of the slots 9 and the locking blocks 10 allows the clamping plate 4 to be quickly and accurately installed on the top of the tank 1. During installation, simply align the locking blocks 10 at the bottom of the clamping plate 4 with the slots 9 at the top of the tank 1, and then gently press down to complete the installation. During disassembly, simply lift the clamping plate 4 upwards to disengage the locking blocks 10 from the slots 9. This connection method is simple and convenient, improving the efficiency of experimental operations. By setting the engaging structure of the slots 9 and the locking blocks 10, the clamping plate 4 and the tank 1 are quickly and stably connected, facilitating the installation and disassembly of the clamping plate 4 by experimental personnel.

[0028] The top of the tank 1 is equipped with a positioning assembly 11 for quick installation and removal of the cathode tube 2 and the anode tube 3. The positioning assembly 11 includes a positioning tube 1101, which is fixedly embedded in the top of the tank 1. The cathode tube 2 and the anode tube 3 are respectively inserted into the positioning tube 1101. Fixing rings 1102 are fixedly sleeved on the outer walls of the cathode tube 2 and the anode tube 3, and are threadedly connected to the outer walls of the positioning tube 1101. The positioning tube 1101 provides precise positioning for the cathode tube 2 and the anode tube 3, allowing them to be accurately installed in the designated positions on the tank 1. The threaded connection between the fixing rings 1102 and the positioning tube 1101 enables quick and convenient installation and removal of the cathode tube 2 and the anode tube 3. During installation, the cathode tube 2 and the anode tube 3 are inserted into the positioning tube 1101, and then the fixing rings 1102 are rotated to secure the threaded connection with the positioning tube 1101. During removal, the fixing rings 1102 are rotated in the opposite direction to remove the cathode tube 2 and the anode tube 3. By setting up the positioning assembly 11 with positioning tube 1101 and fixing ring 1102, the cathode tube 2 and anode tube 3 can be quickly and stably disassembled and assembled, making it convenient for experimental personnel to maintain and replace the electrode tubes.

[0029] A mounting groove 13 is formed on one side wall of the tank 1. Inside the mounting groove 13 is a cooling component 12 for cooling the transfer buffer solution inside the tank 1 to prevent protein denaturation. The cooling component 12 includes a thermoelectric cooler 1201, which is fixedly mounted on the inner wall of one side of the tank 1. A heat dissipation fin 1202 is fixedly connected to one side wall of the thermoelectric cooler 1201 and is embedded inside the mounting groove 13, with one end of the heat dissipation fin 1202 penetrating through the mounting groove 13. The thermoelectric cooler 1201 utilizes the Peltier effect of semiconductor materials to achieve a cooling function. During the transfer process, the thermoelectric cooler 1201 cools the transfer buffer solution inside the tank 1 to prevent protein denaturation due to excessive temperature. The heat dissipation fin 1202 dissipates the heat generated by the thermoelectric cooler 1201 to the external environment, ensuring the normal operation of the thermoelectric cooler 1201. By setting up a cooling component 12 with a semiconductor cooling chip 1201 and heat dissipation fins 1202, the temperature of the transfer buffer is effectively reduced, protecting the activity of the protein and improving the success rate of the transfer experiment.

[0030] Working principle: First, the cathode tube 2 and anode tube 3 are installed on the top of the tank 1 through the positioning assembly 11. The cathode tube 2 and anode tube 3 are inserted into the positioning tube 1101. The fixing ring 1102 is rotated to make it threadedly connected and tightened with the positioning tube 1101, thus completing the installation of the electrode tubes. Then, the moving plate 6 is moved by the adjusting assembly 7 to clamp the PVDF membrane. The handwheel 703 is turned to drive the lead screw 702 to rotate. The rotation of the lead screw 702 causes the movable block 704 to move along the limiting through groove 8, which in turn drives the moving plate 6 to move, clamping the PVDF membrane between the fixed plate 5 and the moving plate 6. The clamping plate 4, which clamps the PVDF membrane and gel, is installed on the top of the tank 1 through the snap-fit ​​structure of the snap-fit ​​block 10 and the snap-fit ​​groove 9.

[0031] During the transfer process, the power is turned on, and the cathode tube 2 and anode tube 3 form an electric field in the tank 1, driving charged protein molecules to migrate from the gel to the PVDF membrane. At the same time, the semiconductor cooling chip 1201 in the cooling component 12 starts to work, cooling the transfer buffer in the tank 1 to prevent protein denaturation. The heat generated by the semiconductor cooling chip 1201 is dissipated to the external environment through the heat dissipation fins 1202. After the transfer is completed, the handwheel 703 is rotated in the opposite direction to move the moving plate 6 to release the PVDF membrane. Then the clamping plate 4 is removed to obtain the PVDF membrane with protein transferred on it.

[0032] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A transfer groove for a PVDF membrane, characterized in that, include: The tank (1) is provided with a cathode tube (2) and an anode tube (3) inside, and the top of the tank (1) is open; A clamping plate (4) is set on the top of the tank (1). Several sets of fixed plates (5) are fixedly connected to the bottom of the clamping plate (4). A movable plate (6) is set on one side of the clamping plate (4) and an adjustment component (7) is set on the top of the clamping plate (4) for moving the movable plate (6) to clamp and release the PVDF membrane.

2. The transfer tank for a PVDF membrane according to claim 1, characterized in that: The adjustment assembly (7) includes a mounting plate (701). Both sets of mounting plates (701) are fixedly mounted on the top of the clamping plate (4). A lead screw (702) is rotatably mounted on the opposite side of the mounting plate (701). One end of the lead screw (702) passes through one set of mounting plates (701) and is fixedly connected to a handwheel (703). Several sets of movable blocks (704) are threaded onto the outer wall of the lead screw (702). The bottom of the movable block (704) passes through the clamping plate (4) and is fixedly connected to the top of the moving plate (6).

3. The transfer tank for a PVDF membrane according to claim 2, characterized in that: The top of the clamping plate (4) is provided with several sets of limiting through grooves (8) that are adapted to the movable block (704), and the movable block (704) is slidably connected to the inside of the limiting through grooves (8).

4. The transfer tank for a PVDF membrane according to claim 3, characterized in that: The top of the groove (1) is provided with several sets of slots (9), and the bottom of the clamp (4) is fixedly connected with several sets of blocks (10) that are adapted to the slots (9). The blocks (10) are engaged inside the slots (9).

5. The transfer tank for a PVDF membrane according to claim 1, characterized in that: The top of the tank (1) is provided with a positioning assembly (11) for quick assembly and disassembly of the cathode tube (2) and the anode tube (3). The positioning assembly (11) includes a positioning tube (1101), which is fixedly embedded in the top of the tank (1). The cathode tube (2) and the anode tube (3) are respectively inserted into the interior of the positioning tube (1101). Fixing rings (1102) are fixedly sleeved on the outer walls of the cathode tube (2) and the anode tube (3), and the fixing rings (1102) are threadedly connected to the outer wall of the positioning tube (1101).

6. The transfer tank for a PVDF membrane according to claim 1, characterized in that: An installation groove (13) is provided on one side wall of the tank (1), and a cooling component (12) is provided inside the installation groove (13) to cool the transfer buffer inside the tank (1) to prevent protein denaturation.

7. The transfer tank for a PVDF membrane according to claim 6, characterized in that: The cooling component (12) includes a thermoelectric cooler (1201), which is fixedly installed on one side inner wall of the tank (1). A heat dissipation fin (1202) is fixedly connected to one side wall of the thermoelectric cooler (1201), and the heat dissipation fin (1202) is fixedly embedded in the inside of the mounting groove (13), with one end of the heat dissipation fin (1202) penetrating through the mounting groove (13).

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