Nanomembrane virus removal pilot plant device

Abstract

The utility model discloses a kind of nanometer membrane virus removal small test device, belong to biological product and blood product production technical field.The device includes filter tank, nanometer membrane and filtrate collection weighing component, the top of the filter tank is connected with air inlet pipe, air pressure detection assembly and air pressure regulating assembly are equipped on the air inlet pipe, and air pressure detection assembly is located between air pressure regulating assembly and filter tank, the liquid inlet of the nanometer membrane is connected with the bottom of filter tank by pipeline, and hydraulic detection assembly A is equipped on the pipeline, the liquid outlet of the nanometer membrane is connected with drain pipe, and hydraulic detection assembly B is equipped on the drain pipe, the filtrate collection weighing component is placed just below the liquid outlet of drain pipe.The real-time data of power gas source pressure value, feed liquid nanofiltration before and after pressure value, reflect the nanofiltration pressure real-time change situation of nanometer membrane;The real-time data record of feed liquid weight after nanofiltration, the change trend of nanofiltration load and nanofiltration effect of nanometer membrane is evaluated by calculation.

Description

Technical Field

[0001] This utility model relates to a nanomembrane virus removal pilot device, belonging to the field of biological products and blood product manufacturing technology. Background Technology

[0002] In the production of biological products and blood products, viruses may be introduced into the raw materials or processing, resulting in the risk of viral or potential viral contamination in the products. Therefore, methods for virus inactivation or removal must be introduced during the production process.

[0003] Nanofiltration is a safe and effective method that can remove viruses while preserving the bioactivity of the product. Currently, mainstream manufacturers typically use this method to remove viruses from biological and blood products. The principle behind nanofiltration for virus removal is based on the trapping of viruses by the membrane pore size. This method can effectively remove lipid-enveloped viruses, non-lipid-enveloped viruses, and parvoviruses. Its ability to remove parvoviruses is particularly noteworthy as it is unattainable by other virus inactivation methods such as S / D inactivation, dry heat inactivation, and pasteurization. Simultaneously, while reliably and effectively removing viruses, nanofiltration also exhibits good protein permeability, avoiding the need for relatively harsh chemical and high-temperature inactivation methods, thus better preserving the integrity and bioactivity of proteins. Typically, nanofiltration is performed after target protein purification and before the sterilization filtration step, further limiting the risk of downstream contamination.

[0004] For example, Chinese patent document CN106823541A discloses a nanomembrane filtration system; Chinese patent document CN116251476A discloses a virus removal filtration system using in-situ sterilized nanomembranes. Both of these filtration systems directly apply nanomembranes to the production of biological products or blood products. However, in actual production, to avoid losses due to large-scale use of nanomembranes, it is necessary to understand their nanofiltration pressure changes, nanofiltration capacity, and nanofiltration effect before large-scale use, in order to make better selections of nanomembranes. Based on this, there is an urgent need to develop a small-scale nanomembrane virus removal device to evaluate the nanofiltration pressure changes, nanofiltration capacity, and nanofiltration effect of nanomembranes. Utility Model Content

[0005] To address the aforementioned technical problems, this invention provides a small-scale device for removing viruses using a nanofilm.

[0006] This utility model is achieved through the following technical solution:

[0007] A pilot device for removing viruses using a nanomembrane includes a filter tank, a nanomembrane, and a filtrate collection and weighing component. The top of the filter tank is connected to an air inlet pipe, which is equipped with a pressure detection component and a pressure regulating component. The pressure detection component is located between the pressure regulating component and the filter tank. The inlet of the nanomembrane is connected to the bottom of the filter tank via a pipe, which is equipped with a hydraulic detection component A. The outlet of the nanomembrane is connected to a drain pipe, which is equipped with a hydraulic detection component B. The filtrate collection and weighing component is placed directly below the outlet of the drain pipe.

[0008] The pressure regulating component is a gas pressure reducing valve.

[0009] The air pressure detection component is a digital pressure gauge.

[0010] The filter tank has a double-layer structure, including an inner wall and an outer wall. A temperature sensor is installed on the inner side of the inner wall. The space between the outer wall and the inner wall is a cavity. A temperature-controlled liquid inlet pipe is installed at the bottom of the cavity, and a temperature-controlled liquid outlet pipe is installed at the top.

[0011] An electric ball valve is installed on the pipeline, and the electric ball valve is located between the hydraulic detection component A and the filter tank, and is arranged close to the filter tank.

[0012] An exhaust pipe is connected to the pipeline near the nanofilm, and a check valve is installed on the exhaust pipe.

[0013] Both hydraulic testing component A and hydraulic testing component B are digital pressure gauges.

[0014] The filtrate collection and weighing component includes a weighing assembly and a filtrate collection container placed on the weighing assembly.

[0015] The weighing component is an electronic balance.

[0016] It also includes a laptop computer, which communicates with the air pressure detection component, hydraulic detection component A, and hydraulic detection component B.

[0017] The beneficial effects of this utility model are as follows:

[0018] First, real-time data on the power air source pressure, the pressure of the feed solution before nanofiltration, and the pressure of the feed solution after nanofiltration can more accurately reflect the real-time changes in the nanofiltration pressure of the nanomembrane. Second, by using real-time data recording of the weight of the post-nanofiltration feed solution measured by the filtrate collection and weighing component, the changing trends of the nanofiltration loading and nanofiltration effect of the nanomembrane at each time point can be evaluated through calculation. This provides reliable data for nanomembrane selection, reduces deviations during process scale-up, and lowers the risk of increased production costs due to misjudgment of the nanofiltration loading caused by large-scale use of nanomembranes. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the structure of this utility model.

[0020] In the diagram: 1-Inlet pipe, 2-Pressure regulating component, 3-Pressure detection component, 4-Filter tank, 5-Temperature-controlled drain pipe, 6-Clamping cavity, 7-Electric ball valve, 8-Pipeline, 9-Temperature-controlled inlet pipe, 10-Hydraulic detection component A, 11-Check valve, 12-Exhaust pipe, 13-Nano membrane, 14-Hydraulic detection component B, 15-Drain pipe, 16-Filtrate collection container, 17-Weighing component, 18-Laptop computer. Detailed Implementation

[0021] The technical solution of this utility model is further described below, but the scope of protection is not limited to what is described.

[0022] like Figure 1 As shown, the present invention discloses a nano-membrane virus removal pilot device, comprising a filter tank 4, a nano-membrane 13, and a filtrate collection and weighing component. The top of the filter tank 4 is connected to an air inlet pipe 1, and the air inlet pipe 1 is provided with an air pressure detection component 3 and an air pressure regulating component 2, with the air pressure detection component 3 located between the air pressure regulating component 2 and the filter tank 4. The liquid inlet of the nano-membrane 13 is connected to the bottom of the filter tank 4 through a pipe 8, and the pipe 8 is provided with a hydraulic detection component A10. The liquid outlet of the nano-membrane 13 is connected to a drain pipe 15, and the drain pipe 15 is provided with a hydraulic detection component B14. The filtrate collection and weighing component is placed directly below the liquid outlet of the drain pipe 15. In use, the liquid before nanofiltration is stored in the filter tank 4; compressed gas is injected into the filter tank 4 through the air inlet pipe 1 as a power source, the pressure of the power source is adjusted by the air pressure regulating component 2, and the pressure of the power source is monitored by the air pressure detection component 3 to provide filtration pressure for the nanomembrane 13; the liquid in the filter tank 4 enters the nanomembrane 13 through the pipe 8 under the action of the power source for nanofiltration, and the liquid after nanofiltration is discharged into the filtrate collection and weighing component through the drain pipe 15. During this process, the pressure of the liquid before nanofiltration is detected by the hydraulic detection component A10, and the pressure of the liquid after nanofiltration is detected by the hydraulic detection component B14 to realize real-time monitoring of the pressure during nanofiltration. At the same time, the weight of the liquid after nanofiltration is also weighed in real time by the filtrate collection and weighing component.

[0023] First, by using real-time data on the power air source pressure, the pressure of the feed liquid before nanofiltration, and the pressure of the feed liquid after nanofiltration, the real-time changes in the nanofiltration pressure of the nanomembrane 13 can be reflected more accurately and precisely. Second, by using real-time data recording of the weight of the feed liquid after nanofiltration measured by the filtrate collection and weighing component, the changing trends of the nanofiltration capacity and nanofiltration effect of the nanomembrane 13 at each time point can be evaluated through calculation. This provides reliable data for the selection of the nanomembrane 13, reduces deviations in the process scale-up process, and lowers the risk of increased production costs due to misjudgment of the nanofiltration capacity caused by large-scale use of the nanomembrane 13.

[0024] The pressure regulating component 2 is a gas pressure reducing valve.

[0025] The air pressure detection component 3 is a digital pressure gauge.

[0026] The filter tank 4 has a double-layer structure, including an inner wall and an outer wall. A temperature sensor is installed on the inner side of the inner wall. The space between the outer wall and the inner wall is a cavity 6. A temperature-controlled inlet pipe 9 is installed at the bottom of the cavity 6, and a temperature-controlled outlet pipe 5 is installed at the top. In use, a liquid addition port is opened at the top of the filter tank 4, and a sealing cap is installed at the liquid addition port. The sealing cap can be opened to add liquid to the filter tank 4 through the liquid addition port. Since the filtration time of the nanomembrane 13 is generally long, and temperature may affect the nanofiltration pressure, nanofiltration capacity, and nanofiltration effect test of the nanomembrane 13, this utility model uses a double-layer filter tank 4 to hold the liquid, and uses a temperature sensor to monitor the temperature of the liquid in the filter tank 4. At the same time, the temperature-controlled inlet pipe 9 and the temperature-controlled outlet pipe 5 are connected to refrigeration equipment such as a condenser, so that the cooling medium enters from the bottom and exits from the top in the cavity 6, thereby controlling the temperature of the liquid in the filter tank 4 and achieving the purpose of controlling the temperature of the liquid nanofiltration process. The temperature sensor communicates with the laptop.

[0027] An electric ball valve 7 is installed on the pipeline 8, and the electric ball valve 7 is located between the hydraulic detection component A10 and the filter tank 4, and is arranged close to the filter tank 4. In use, the electric ball valve 7 is connected to the laptop computer 18 to control the flow of liquid out of the filter tank 4.

[0028] An exhaust pipe 12 is connected to the pipe 8 near the nanofilm 13, and a check valve 11 is installed on the exhaust pipe 12.

[0029] Both the hydraulic detection component A10 and the hydraulic detection component B14 are digital pressure gauges.

[0030] The filtrate collection and weighing component includes a weighing assembly 17 and a filtrate collection container 16 placed on the weighing assembly 17. In use, the filtrate collection container 16 is used to receive and collect the nanofiltration filtrate.

[0031] The weighing component 17 is an electronic balance. In use, the electronic balance is connected to a laptop computer and is used to weigh the nanofiltration solution.

[0032] It also includes a laptop computer 18, which is in communication connection with the air pressure detection component 3, the hydraulic detection component A10 and the hydraulic detection component B14.

[0033] The working principle or usage process of the nanomembrane virus removal pilot device of this utility model is as follows:

[0034] Compressed gas is injected into the filter canister 4 through the air inlet pipe 1 as a power source. The pressure of the power source is adjusted by the air pressure regulating component 2, and the pressure of the power source is monitored by the air pressure detection component 3 to provide filtration pressure for the nanomembrane 13.

[0035] Under the action of a power air source, the liquid in the filter tank 4 enters the nanomembrane 13 through the pipe 8 for nanofiltration. The filtered liquid is then discharged into the filtrate collection and weighing component through the drain pipe 15. During this process, the pressure of the liquid before nanofiltration is detected by the hydraulic detection component A10, and the pressure of the liquid after nanofiltration is detected by the hydraulic detection component B14, so as to realize real-time monitoring of the pressure during the nanofiltration process. At the same time, the weight of the filtered liquid is also weighed in real time by the filtrate collection and weighing component.

[0036] The laptop receives real-time data on the power air source pressure, the pressure of the feed liquid before nanofiltration, and the pressure of the feed liquid after nanofiltration. This data accurately reflects the real-time changes in the nanofiltration pressure of the nanomembrane 13. Simultaneously, the laptop receives real-time data on the weight of the post-nanofiltration feed liquid measured by an electronic balance, and calculates and evaluates the changing trends of the nanofiltration capacity and nanofiltration effect of the nanomembrane 13 at each time point.

[0037] Using laptops to automatically collect and calculate data reduces human error and lowers the workload for staff.

Claims

1. A pilot-scale device for removing viruses using a nanomembrane, characterized in that: The device includes a filter tank (4), a nanomembrane (13), and a filtrate collection and weighing component. The top of the filter tank (4) is connected to an air inlet pipe (1). The air inlet pipe (1) is equipped with a pressure detection component (3) and a pressure regulating component (2). The pressure detection component (3) is located between the pressure regulating component (2) and the filter tank (4). The inlet of the nanomembrane (13) is connected to the bottom of the filter tank (4) through a pipe (8). The pipe (8) is equipped with a hydraulic detection component A (10). The outlet of the nanomembrane (13) is connected to a drain pipe (15). The drain pipe (15) is equipped with a hydraulic detection component B (14). The filtrate collection and weighing component is placed directly below the outlet of the drain pipe (15).

2. The nanofilm virus removal pilot device as described in claim 1, characterized in that: The pressure regulating component (2) is a gas pressure reducing valve.

3. The nanofilm virus removal pilot device as described in claim 1, characterized in that: The air pressure detection component (3) is a digital pressure gauge.

4. The nanofilm virus removal pilot device as described in claim 1, characterized in that: The filter tank (4) has a double-layer structure, including an inner wall and an outer wall. A temperature sensor is provided on the inner side of the inner wall. The space between the outer wall and the inner wall is a cavity (6). A temperature-controlled liquid inlet pipe (9) is provided at the bottom of the cavity (6), and a temperature-controlled liquid outlet pipe (5) is provided at the top.

5. The nanofilm virus removal pilot device as described in claim 1, characterized in that: An electric ball valve (7) is installed on the pipeline (8), and the electric ball valve (7) is located between the hydraulic detection component A (10) and the filter tank (4), and is arranged close to the filter tank (4).

6. The nanofilm virus removal pilot device as described in claim 1, characterized in that: An exhaust pipe (12) is connected to the pipe (8) near the nanofilm (13), and a check valve (11) is installed on the exhaust pipe (12).

7. The nanofilm virus removal pilot device as described in claim 1, characterized in that: Both the hydraulic detection component A (10) and the hydraulic detection component B (14) are digital pressure gauges.

8. The nanofilm virus removal pilot device as described in claim 1, characterized in that: The filtrate collection and weighing component includes a weighing assembly (17) and a filtrate collection container (16) placed on the weighing assembly (17).

9. The nanofilm virus removal pilot device as described in claim 8, characterized in that: The weighing component (17) is an electronic balance.

10. The nanofilm virus removal pilot device as described in claim 1, characterized in that: It also includes a laptop computer (18), which is connected in communication with the air pressure detection component (3), the hydraulic detection component A (10) and the hydraulic detection component B (14).

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