A bioreactor

Abstract

The utility model discloses a bioreactor, adopt aeration part to the inside of container is supplied with gas, and aeration part surrounds the circumferential direction of impeller, make the gas of the gas output surface output of aeration part can be even diffusion to the inside of container by impeller fast, set up several gas guide plates in the inside of aeration part to form several gas grooves with the communication of air inlet, and every gas groove has corresponding air outlet hole, further improves the evenness and dispersity of air outlet, to promote gas to be beaten by impeller in time and scatter, simultaneously, set up the communication groove in the gas cavity, and the communication groove forms the cross -communication part with several gas grooves, improves the speed and evenness of gas from air inlet and enters each gas groove, makes the air output of every air outlet hole as consistent as possible, and the gas guide plate along the gas dissipation direction extension still can support the space in the gas cavity, is favorable to reduce the possibility that the first wall with air outlet hole happens deformation collapse, to guarantee that gas disperses smoothly.

Description

Technical Field

[0001] This utility model relates to the field of biological treatment technology, and in particular to a bioreactor. Background Technology

[0002] In the current biopharmaceutical process, in order to commercialize and mass-produce biological products such as enzymes, antibodies, or antibiotics, animal, plant, or microbial cells are usually placed in a bioreactor for sterile culture. During this process, the biological fluid is controlled by means of controlling the mixing system, gas supply, and monitoring by sensor probes to obtain the desired biological product.

[0003] The disposable container disclosed in Chinese invention patent CN1316013C includes a foldable bag and a top plate. The top of the bag is sealed around the edge of the top plate. It also includes an impeller placed inside the bag. The top plate has several holes, one of which can be used for gas supply. After the gas enters the bag through the hole, it is driven by the impeller to mix with the liquid, so that the gas dissolves in the liquid and maintains a uniform environment throughout the entire interior of the bag.

[0004] For example, a cell culture device disclosed in Chinese utility model patent CN204356344U includes a top cover, a shell, an inlet tube, a magnetic stirrer, a base, a magnetic stirrer, a breathing tube, a disposable fluorescent electrical interface, an outlet tube, a cell adsorption plate, and a base. The breathing tube is located in the middle of the device, and the stirrer is located at the bottom of the device. The breathing tube is equipped with a custom interface, through which the user can introduce gases such as nitrogen, air, and oxygen into the container and mix the gas and liquid through the stirrer.

[0005] Therefore, one of the most important functions of a bioreactor is to achieve gas-liquid mixing through a stirring device, promote the mass transfer of bubbles through the gas-liquid (cell culture medium) interface, thereby enabling biological culture within the container to obtain the desired biological products.

[0006] However, both Chinese invention patent CN1316013C and Chinese utility model patent CN204356344U supply gas to the container through a single opening or interface, and then use a stirring device to disperse the bubbles. The above-mentioned gas supply method, which is concentrated in a single opening or interface, is prone to the gas not being dispersed in time and in a timely manner, which leads to uneven gas distribution in the container and affects the gas-liquid mixing efficiency. Utility Model Content

[0007] In view of the shortcomings of the existing technology, the purpose of this utility model is to provide a bioreactor that solves the problem of uneven gas distribution in existing bioreactors.

[0008] To achieve the above objectives, the present invention adopts the following technical solution:

[0009] A bioreactor includes a container, an aeration section, and an impeller disposed within the container, wherein the aeration section is configured such that at least its outlet surface is located within the container.

[0010] The aeration section is distributed circumferentially around the impeller. The aeration section includes a first wall and a second wall arranged opposite to each other, an air cavity located between the first wall and the second wall, and an air inlet communicating with the air cavity. The surface of the first wall facing the impeller is the air outlet surface, and the air inlet is located on the second wall.

[0011] The air chamber is provided with a plurality of spaced air guide plates. The air guide plates extend between the first wall and the second wall along the gas dispersion direction, and air grooves are formed between adjacent air guide plates. Several of the air grooves are connected to the air inlet.

[0012] The air guide plate has a second notch, and a plurality of the second notches form the connecting groove. The connecting groove intersects with and connects with a plurality of the air grooves to form a cross-connecting part.

[0013] The first wall has multiple air outlets in the area corresponding to the air groove that communicate with the air cavity.

[0014] This bioreactor employs an aeration section to supply gas to the interior of the container. The aeration section surrounds the impeller circumferentially, allowing the gas output from the outlet surface of the aeration section to be quickly and evenly dispersed by the impeller and diffused into the container interior. Several guide plates are installed inside the aeration section, with the gas dispersion direction indicating that the gas moves from the inlet to the outlet. The inlet is located on the second wall, and the outlet is located on the first wall. Therefore, the guide plates extend between the first and second walls, preferably along the impeller axial direction. These guide plates form several gas grooves communicating with the inlet, each with a corresponding outlet, further improving the uniformity and dispersion of the gas output and ensuring timely dispersion by the impeller. Simultaneously, a connecting groove is installed within the gas chamber, intersecting and connecting with several gas grooves to form a cross-connecting section. This means the gas grooves and the connecting grooves extend in different directions, guiding the gas flow in different directions, which is beneficial for promoting... This design ensures uniform three-dimensional gas distribution within the gas cavity, reducing dead zones. Simultaneously, it increases the diversity of gas flow paths, promoting timely and rapid gas diffusion. Specifically, after entering the gas cavity through the inlet, some gas can directly enter the gas slots, while some can directly enter the connecting slots. Then, through the cross-connecting sections, the gas is distributed to several slots, forming a "main channel-branch" flow pattern. This delays local saturation and helps prevent uneven gas distribution caused by direct injection into a single slot. Furthermore, as the gas flows through the connecting slots and gas slots, the pressure gradually decreases along the path. However, the cross-connecting sections allow adjacent slots to indirectly share pressure, reducing pressure differences between slots and ensuring stable gas pressure within the gas cavity. Consequently, the gas output from each outlet on the outlet surface is roughly balanced. The guide plate, extending along the gas dispersion direction, also supports the space within the gas cavity, reducing the possibility of deformation and collapse of the first wall with outlet holes, thus ensuring smooth gas dispersion.

[0015] Preferably, the axial projection of the cross-connecting part coincides with the axial projection of the air inlet; the gas jet entering from the air inlet directly impacts the intersection of the gas slot and the connecting slot, using initial kinetic energy to quickly establish branch channel pressure, thereby causing the gas to diffuse through the cross-connecting part to multiple gas slots; it also causes the corresponding air inlet in the cross-connecting part to form a pressure focus, based on the principle of prioritizing gas supply from the high-pressure area to the low-pressure area, automatically compensating for the resistance differences of each gas slot, promoting gas flow balance. Thus, the gas entering from the air inlet can quickly enter the cross-connecting part and be evenly distributed in each gas slot, avoiding gas concentration in a few gas slots, which is beneficial to improving gas dispersion.

[0016] Preferably, the connecting groove extends along a first direction, and each gas groove extends along a second direction. The angle between the first direction and the second direction is 80°-100°, so that the extension length of each gas groove along the second direction is similar, and the gas capacity of each gas groove is also relatively close. The preferred angle between the first direction and the second direction is 90°, so that the connecting groove and the gas groove form a three-dimensional flow field structure. The connecting groove can diffuse the gas to the transverse section between the first wall and the second wall, while the gas groove can guide the gas from the second wall to the depth direction of the first wall, forming a three-dimensional flow network of "transverse distribution - vertical penetration", which improves the uniformity of gas distribution in the gas cavity, and the gas can quickly diffuse into each gas groove and fill each gas groove.

[0017] Preferably, at least one baffle is provided in the air cavity, a diffuser is provided between the air inlet and the baffle to allow gas to enter the air cavity from the air inlet, and an escape section is provided between the diffuser and the air slot to allow gas to enter each of the air slots from the diffuser.

[0018] The function of the baffle is to further disperse the gas entering from the air inlet. After the gas enters the diffuser, it can diffuse for the first time, and then hit the baffle and enter each gas slot through the diffuser. This prevents the gas from being concentrated near the air inlet and overflowing, which helps to improve the dispersion of the gas. In addition, the baffle can also improve the structural rigidity of the aeration section and prevent the gas jet from directly impacting the air guide plate and causing the air guide plate to deform.

[0019] Preferably, the baffle is clearance-fitted with the second wall and the air inlet, the axial projection of the baffle covers the air inlet, the baffle includes a lower surface facing the opening, an upper surface axially opposite to the lower surface, and a side surface connecting the upper surface and the lower surface, the axial space between the lower surface of the baffle and the air inlet forms the diffuser, and the first gap between the lower surface of the baffle and the second wall forms at least part of the diffuser.

[0020] The baffle and the air inlet are fitted with a gap, which will not interfere with the air intake. At the same time, the axial projection of the baffle covers the air inlet, and the air guide plate extends from the first wall to the second wall. Therefore, the air inlet, air guide plate, air trough, connecting trough and baffle are arranged opposite to each other. After the gas jet enters the diffuser, it hits the baffle and is forced to be diverted by the baffle, and then diffuses to the side into the escaping part, so as to promote the gas to be evenly distributed into each air trough. The axial arrangement of the baffle and the air inlet can also improve the space utilization rate in the air chamber, shorten the flow path of the gas in the diffuser, so as to promote the rapid flow of gas in the entire air trough and escape to the bioreactor. At the same time, it also makes the structure of the aeration part more compact.

[0021] Preferably, at least a portion of the air guide plate has a first notch, and a plurality of the first notches form a receiving cavity for accommodating the baffle; this allows the baffle to be embedded in the air guide plate, facilitating the positioning between the baffle and the air guide plate, and also makes the distance between the baffle and the air groove shorter, resulting in a more compact structure and helping to shorten the flow path of the gas in the escaping section.

[0022] Preferably, the sidewall of the receiving cavity and the side of the baffle have a first gap, the first gap forming at least part of the escaping portion to connect the diffuser portion and all the air slots.

[0023] After the gas enters the diffuser through the inlet and hits the baffle, it diffuses and moves to the side of the baffle into the first gap, and then quickly enters the gas groove between the guide plates. The surface of the baffle disperses the gas, preventing the gas from concentrating in the diffuser. The side of the baffle can correspond to most or even all of the gas grooves to improve the uniformity of gas distribution in the gas chamber, which is beneficial to improving the dispersion of gas escape.

[0024] Preferably, the air guide plate includes a fixed end fixed to the first wall and a free end away from the first wall, and the second wall is provided with a positioning part for positioning the free end of the air guide plate and / or the baffle, and at least a portion of the positioning part and the baffle have a second gap, the second gap forming at least a portion of the escaping part.

[0025] The second gap, located between the positioning part and the baffle, is situated on the outer periphery of the diffuser. This allows the gas entering the diffuser from the inlet to flow to the side after hitting the baffle. It can either directly enter the gas groove connected to the second gap or enter the first gap connected to the second gap. These multiple flow paths further disperse the gas, ensuring that there is gas in each gas groove and improving the dispersion of the escaping gas.

[0026] Preferably, the positioning part is spaced apart from the air inlet, and the first gap between the lower surface of the baffle and the second wall circumferentially of the air inlet forms part of the escaping part;

[0027] At least a portion of the positioning portion is located on the lower surface of the baffle and is clearance-fitted with the lower surface of the baffle to form a portion of the escaping portion.

[0028] The first gap between the lower surface of the baffle and the second wall is located on the outer periphery of the diffuser. This allows the gas entering the diffuser through the inlet to flow to the side after hitting the baffle. It can directly enter the gas groove connected to the first gap, or enter the first void connected to the first gap, or enter the second void connected to the first gap. Multiple flow paths further disperse the gas to ensure that there is gas in each gas groove, thereby improving the dispersion of the gas escape.

[0029] Preferably, the air inlet is offset from the baffle, the diffuser is located on the side of the baffle, and the baffle is spaced apart from the first wall or the second wall to form the escaping section, so as to connect the air inlet and each of the air slots; after the positions of the air inlet and the baffle are offset, that is, the flow direction of the gas entering from the air inlet is also offset from the baffle, which helps to avoid the gas directly impacting the baffle during the process of entering from the air inlet, thereby helping to avoid the baffle from deforming or displacing due to excessive gas pressure or long-term impact, thereby hindering the dispersion process of gas in the air cavity.

[0030] Preferably, the positioning part includes a positioning protrusion and a limiting groove. The limiting groove is located between adjacent positioning protrusions in the radial direction of the impeller. The area of ​​the free end of the air guide plate without the first notch is engaged with the limiting groove. The air groove is provided corresponding to the positioning protrusion. This limits the free end of the air guide plate in the limiting groove, preventing the air guide plate from shifting or deviating and reducing the air guiding effect. Furthermore, since the air guide plate extends from the first wall and the positioning part is located on the second wall, the engagement between the air guide plate and the limiting groove achieves indirect connection and positioning between the first wall and the second wall, ensuring the overall structural stability of the aeration part.

[0031] Preferably, the positioning part includes a positioning groove, and the baffle is provided with a positioning member protruding from the lower surface of the baffle. The positioning member is engaged with the positioning groove to position the baffle in the air cavity, so as to prevent the baffle from shifting or deviating and hindering the flow of gas in the escaping part.

[0032] Preferably, the positioning element is located on the lower surface of the baffle.

[0033] The positioning part further includes positioning protrusions, at least a portion of which are located below the lower surface of the baffle and spaced apart circumferentially on the impeller to form the positioning grooves. This brings the positioning protrusions closer to the air inlet, resulting in a shorter distance between the stress point and the positioning point when the baffle is impacted by gas from the air inlet, and a smaller lever arm. This allows the load on the baffle to be directly transferred to the positioning protrusions and the second wall, ensuring the stability of the baffle; or...

[0034] The positioning element is located at both ends of the baffle along the radial direction of the impeller, and the positioning groove is also located on the second wall in the area corresponding to both ends of the baffle along the radial direction of the impeller. The space between the lower surface of the baffle and the second wall is larger, which can accommodate more gas and improve space utilization.

[0035] Preferably, the length of the air guide plate along the impeller axis is L1, and the axial length of the air guide plate extending to the inner end of the limiting groove is L2, where L2 / L1 = 0.1-0.6. The value of L2 / L1 reflects the balance between the usable volume of the air groove and the limiting effect. If the value of L2 / L1 is too large, the limiting groove is too deep, the length of the air guide plate inserted into the limiting groove is too long, the usable volume of the remaining air groove is too small, the positioning part occupies too much air cavity space, which can easily hinder gas flow and reduce space utilization. If the value of L2 / L1 is too small, the limiting groove is too shallow, the limiting effect on the air guide plate is poor, which can easily lead to the displacement or deviation of the air guide plate and reduce the air guiding effect.

[0036] Preferably, the thickness of the air guide plate in the radial direction of the impeller is d1, and the width of the air groove in the radial direction of the impeller is d2, where d1 / d2 = 0.5-3. The value of d1 / d2 reflects the balance between the structural strength of the air guide plate and the usable volume of the air groove. If the value of d1 / d2 is too large, the radial width of the air groove will be too small, increasing the difficulty for gas to enter the air groove and easily hindering gas flow. If the value of d1 / d2 is too small, the air guide plate will be too thin, resulting in poor support and little support for the space inside the air cavity. This can easily cause the air cavity to deform or collapse under external pressure, thereby hindering airflow.

[0037] Preferably, the projection of the baffle is offset from the projection of the air outlet in the axial direction of the impeller.

[0038] In some embodiments, since the baffle is positioned corresponding to the air inlet and the connecting groove, the gas entering from the air inlet moves to the side after hitting the baffle and bypasses the baffle, concentrating in the connecting groove, and directly escapes from the air outlet in the area corresponding to the baffle of the air groove, instead of moving to the area outside the baffle of the air groove and escaping from the corresponding air outlet, resulting in uneven gas distribution. By misaligning the projection of the baffle with the projection of the air outlet, after the gas entering from the air inlet hits the baffle and moves to the side, some of the gas directly enters the area on both sides of the baffle of the air groove and is discharged from the air outlet in that area, while some of the gas bypasses the baffle and enters the connecting groove and then enters each air groove. Since no air outlet is provided in the area corresponding to the air groove and the connecting groove, the gas is forced to move to the area outside the baffle of the air groove and then be discharged from the corresponding air outlet, thereby promoting the uniformity of gas distribution and escape in the air groove.

[0039] Preferably, multiple air outlets on one of the aeration sections are radially distributed around the center of the air inlet; several aeration sections are radially distributed around the center of the impeller, and the positions of the aeration sections and the positions of their air outlets are distributed around the impeller to ensure uniform gas distribution around the impeller. The impeller further diffuses the gas during rotation, thereby improving the gas uniformity in the entire bioreactor.

[0040] Preferably, several aeration sections are connected by a connecting plate to form a whole, which has a larger volume and area than a single aeration section. Therefore, when the container vibrates due to the rotation of the impeller, each aeration section can maintain a stable position. If the aeration section is connected to the impeller base through the connecting plate, the volume and stability of the impeller base can be increased, thereby reducing the vibration of the impeller itself and that transmitted to the container. This not only ensures the stability of the gas output from the aeration section, but also ensures the relative positional stability of the impeller and the aeration section, so that the impeller can disperse the gas released by the aeration section in time and improve the gas-liquid mixing efficiency.

[0041] Preferably, the first wall, the second wall, and the air guide plate are all made of rigid polymer, which has good compatibility and low assembly difficulty.

[0042] Preferably, the container is a bag. When the bag is fixed inside a rigid container outside the bioreactor, the second wall is attached to the inner wall of the rigid container, and the two ends of the air guide plate are respectively pressed against the first wall and the second wall. The container is located inside the rigid container outside the bioreactor, and the second wall is attached to the inner wall of the rigid container so that the rigid container provides mechanical support for the second wall. This improves the fixation effect of the air guide plate, and the air guide plate supports the first and second walls, enhancing the support effect on the air chamber and ensuring gas flow within the air chamber; it also ensures the installation stability of the aeration section and the stability of the air supply.

[0043] In summary, compared with the prior art, the present invention has at least the following beneficial effects:

[0044] This bioreactor employs an aeration section to supply gas to the interior of the container. The aeration section surrounds the impeller circumferentially, allowing the gas output from the outlet surface of the aeration section to be quickly and evenly dispersed by the impeller and diffused into the container interior. Several air guide plates are installed inside the aeration section to form several gas channels communicating with the air inlet. Each gas channel has a corresponding air outlet, further improving the uniformity and dispersion of the gas output and ensuring timely dispersion by the impeller. Simultaneously, a connecting groove is installed within the gas chamber, intersecting and connecting with several gas channels to form a cross-connecting section. This means the gas channels and connecting grooves extend in different directions, guiding the gas flow in different directions, which helps to promote the uniformity of the three-dimensional gas distribution within the gas chamber and reduces dead zones. Furthermore, it increases the diversity of gas flow paths, thereby promoting gas... The timely and rapid diffusion means that after the gas enters the gas chamber through the inlet, some can directly enter the gas slots, and some can directly enter the connecting slots. Then, through the cross-connecting parts, it is distributed to several gas slots, forming a "main channel-branch" flow pattern, which delays local saturation and helps to avoid uneven gas output caused by direct injection of gas into a single gas slot. In addition, when the gas flows in the connecting slots and gas slots, the pressure gradually decreases along the path, but the cross-connecting parts allow adjacent gas slots to indirectly share pressure, reducing the pressure difference between gas slots and ensuring stable gas pressure in the gas chamber. As a result, the gas output of each gas outlet on the gas outlet surface is roughly balanced. Furthermore, the gas guide plate extends along the gas dispersion direction and can also support the space in the gas chamber, which helps to reduce the possibility of deformation and collapse of the first wall with gas outlet holes, thus ensuring smooth gas dispersion. Attached Figure Description

[0045] To more clearly illustrate the specific embodiments of this utility model or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0046] Figure 1 This is a schematic diagram of the structure of the bioreactor according to an embodiment of the present invention;

[0047] Figure 2 This is a schematic diagram of the aeration section and impeller in an embodiment of the present invention;

[0048] Figure 3 This is a schematic diagram of the aeration section in an embodiment of the present invention;

[0049] Figure 4 This is a schematic diagram of the aeration section from another angle in an embodiment of the present invention;

[0050] Figure 5 The aeration section of Embodiment 1 of this utility model is along Figure 4 A schematic diagram of the cross-sectional structure at point AA;

[0051] Figure 6 This is a schematic diagram of the structure of the first wall and the air guide plate in Embodiment 1 of this utility model;

[0052] Figure 7 This is a schematic diagram of the structure of the second wall and the positioning part in Embodiment 1 of this utility model;

[0053] Figure 8 The aeration section of Embodiment 2 of this utility model is along Figure 4 A schematic diagram of the cross-sectional structure at point BB;

[0054] Figure 9 The aeration section of Embodiment 3 of this utility model is along Figure 4 A schematic diagram of the cross-sectional structure at point BB;

[0055] Figure 10 This is a schematic diagram of the structure of the first wall and the air guide plate in Embodiment 3 of this utility model;

[0056] Figure 11 This is a schematic diagram of the structure of the second wall and the positioning part in Embodiment 3 of this utility model;

[0057] Figure 12 This is a schematic diagram of the baffle structure in Embodiment 3 of this utility model;

[0058] Figure 13 The aeration section of Embodiment 3 of this utility model is along Figure 4 A schematic diagram of the cross-sectional structure at point CC;

[0059] Figure 14 The aeration section of Embodiment 4 of this utility model is along Figure 4 A schematic diagram of the cross-sectional structure at point BB;

[0060] Figure 15 The aeration section of Embodiment 5 of this utility model is along Figure 4 A schematic diagram of the cross-sectional structure at point BB;

[0061] Figure 16 This is a schematic diagram of the structure of the second wall and the positioning part in Embodiment 5 of this utility model;

[0062] Figure 17 The aeration section of Embodiment Six of this utility model is along Figure 4 A schematic diagram of the cross-sectional structure at point BB;

[0063] Figure 18 This is a schematic diagram of the structure of the second wall and the positioning part in Embodiment Six of this utility model;

[0064] Figure 19The aeration section of Embodiment Seven of this utility model is along Figure 4 A schematic diagram of the cross-sectional structure at point BB;

[0065] Figure 20 This is a schematic diagram showing the cooperation between the second wall and the baffle in Embodiment 7 of this utility model;

[0066] Figure 21 The aeration section of Embodiment 8 of this utility model is along Figure 4 A schematic diagram of the cross-sectional structure at point AA;

[0067] Figure 22 This is a schematic diagram of the structure of the first wall and the air guide plate in Embodiment 8 of this utility model;

[0068] Figure 23 This is a schematic diagram of the structure of the second wall and the positioning part in Embodiment 8 of this utility model;

[0069] Figure 24 This is a schematic diagram of the aeration section and impeller in Embodiment 9 of this utility model;

[0070] Figure 25 This is a schematic diagram of the structure of the first wall and the connecting plate in Embodiment 9 of this utility model;

[0071] Figure 26 This is a schematic diagram of the structure of the second wall and the connecting plate in Embodiment 9 of this utility model;

[0072] Figure 27 This is a structural schematic diagram of the second wall and the connecting plate from another angle in Embodiment 9 of this utility model.

[0073] Explanation of reference numerals in the attached figures

[0074] 1. Container; 2. Impeller; 201. Impeller base; 3. Aeration section; 4. Rigid container;

[0075] 10. First wall; 11. Vent surface; 12. Vent hole;

[0076] 20. Air guide plate; 21. Air groove; 22. First notch; 23. Second notch; 24. Connecting groove; 25. Cross-connecting part; 26. Receiving cavity; 27. Fixed end; 28. Free end;

[0077] 30. Second wall; 31. Air inlet; 32. Positioning part; 321. Positioning protrusion; 322. Limiting groove; 323. Positioning groove;

[0078] 40. Baffle; 41. Diffusion section; 42. Exhaust section; 421. First gap; 422. First void; 423. Second void; 43. Positioning element;

[0079] 50. Air cavity;

[0080] 60. Connecting disk; 61. First disk body; 62. Second disk body. Detailed Implementation

[0081] The technical solution of this utility model will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this utility model. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.

[0082] In the description of this utility model, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the accompanying drawings and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0083] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0084] like Figure 1 As shown, the bioreactor of this embodiment includes a container 1, an aeration section 3, and an impeller 2 disposed within the container 1. The aeration section 3 is configured such that at least the air outlet surface 11 is placed within the container 1. The aeration section 3 supplies air to the container 1, and the impeller 2 stirs the liquid and gas within the container 1 to achieve gas-liquid mixing. The structure and distribution of the aeration section 3 have a significant impact on the gas-liquid mixing effect. Figure 2 As shown, in several embodiments of this utility model, the aeration section 3 can be distributed circumferentially around the impeller 2, or the aeration section can be a complete ring distributed circumferentially around the impeller 2, so that the gas output from the air outlet surface 11 of the aeration section 3 can be quickly and evenly diffused into the interior of the container 1 by the impeller 2. The aeration section 3 can be separately arranged from the impeller 2, or it can be connected to the impeller 2.

[0085] As a preferred option, such as Figure 2As shown in the figure, the dotted line represents the air inlet of the aeration section 3. Multiple air outlets 12 on the air outlet surface 11 of the aeration section 3 are radially distributed around the center of the air inlet 31, naturally forming a centrally symmetrical flow field, avoiding the airflow from deflecting to one side due to inertia and improving the uniformity of distribution. Similarly, several aeration sections 3 are radially distributed around the center of the impeller 2, ensuring uniform gas distribution around the impeller 2. The impeller 2 further diffuses the gas during rotation, thereby improving the gas uniformity in the entire bioreactor. At the same time, the radial layout is naturally adapted to cylindrical or spherical containers, maximizing the use of radial space.

[0086] Figure 1 and Figure 2 The impeller's axial and radial directions are shown in the figures. In several embodiments of this utility model, the axial direction refers to the impeller's axial direction, and the radial direction refers to the impeller's radial direction.

[0087] like Figures 3 to 27 In the embodiments shown, the aeration section 3 includes a first wall 10 and a second wall 30 arranged opposite to each other, an air cavity 50 located between the first wall 10 and the second wall 30, and an air inlet 31 communicating with the air cavity 50. The surface of the first wall 10 facing the impeller 2 is the air outlet surface 11. The air inlet 31 is located on the second wall 30. A plurality of spaced air guide plates 20 are provided in the air cavity 50. The air guide plates 20 extend between the first wall 10 and the second wall 30 along the gas dispersion direction, and air grooves 21 are formed between adjacent air guide plates 20. A plurality of air grooves 21 are communicating with the air inlet 31. The air grooves 21 located between the air guide plates 20 are communicating with the air inlet 31, and a plurality of air outlet holes 12 communicating with the air cavity 50 are provided on the area of ​​the first wall 10 corresponding to the air grooves 21.

[0088] The gas dispersion direction refers to the movement of gas from the inlet to the outlet 12. The inlet 31 is located on the second wall 30, and the outlet is located on the first wall 10. Therefore, the guide plate 20 extends between the first wall 10 and the second wall 30, that is, the guide plate 20 extends from the first wall 10 to the second wall 30, or it can extend from the second wall 30 to the first wall 10. Preferably, the guide plate 20 extends along the impeller axis between the first wall 10 and the second wall 30.

[0089] The air guide plate 20 has a second notch 23, and several second notches 23 form a connecting groove 24. The connecting groove 24 intersects and connects with several air grooves 21 to form a cross-connecting part 25. That is, the air grooves 21 and the connecting grooves 24 extend in different directions, which can guide the gas flow in different directions. This is beneficial to promoting the uniformity of the three-dimensional distribution of gas in the air cavity and reducing the flow dead zone. At the same time, it also increases the diversity of gas flow paths, thereby promoting the timeliness and speed of gas diffusion. That is, after the gas enters the air cavity 50 from the air inlet 31, some of it can directly enter the air grooves 21, and some of it can directly enter the connecting grooves 24. Then, it is distributed to several air grooves 21 through the cross-connecting part 25, forming a "main channel-branch" flow pattern. This design delays local saturation, which helps avoid uneven gas output caused by direct injection of gas into a single gas slot 21. In addition, as the gas flows through the connecting slot 24 and the gas slot 21, the pressure gradually decreases along the flow path, but the cross-connecting part 25 allows adjacent gas slots 21 to indirectly share pressure, reducing the pressure difference between gas slots 21 and ensuring stable gas pressure within the gas chamber 50. This also improves the uniformity of gas entering each gas slot 21 from the inlet 31, making the gas output of each outlet 12 as consistent as possible. Furthermore, the guide plate 20, extending between the first wall 10 and the second wall 30, can also support the space within the gas chamber 50, which helps reduce the possibility of deformation and collapse of the first wall 10 with the outlet 12, thus ensuring smooth gas dissipation.

[0090] The internal structure of the aeration section 3 varies, but all aim to improve the dispersion of the emitted gas. The internal structure of the aeration section 3 is described below using several embodiments as examples. It should be noted that the cross-sectional positions and directions in the cross-sectional diagrams of each embodiment are different. Figure 4 The cross-sectional position and orientation are taken into account.

[0091] Example 1

[0092] like Figure 5 , 6 As shown in Figure 7, the connecting groove 24 is located axially above the air inlet 31. The axial projection of the cross connecting part 25 coincides with the axial projection of the air inlet 31. The gas jet entering from the air inlet 31 directly impacts the intersection of the gas groove 21 and the connecting groove 24, using the initial kinetic energy to quickly establish the branch flow channel pressure, thereby allowing the gas to diffuse through the cross connecting part 25 to multiple gas grooves 25. It also makes the corresponding air inlet 21 in the cross connecting part 25 form a pressure focus. Based on the principle of prioritizing gas supply from the high pressure area to the low pressure area, it automatically compensates for the resistance difference of each gas groove 21, promoting the gas flow balance. Thus, the gas entering from the air inlet 31 can quickly enter the cross connecting part 25 and then disperse into several gas grooves 21 connected to the connecting groove 24, avoiding the gas from concentrating in a few gas grooves 21, which is beneficial to improving the gas dispersion.

[0093] Preferably, in this embodiment, the air inlet 31 is located in the central region of the second wall 30, corresponding to the middle region of the connecting groove 24, so that the distance between the air inlet 31 and the two ends of the connecting groove 24 is approximately the same, so as to ensure that the air intake of all the air grooves 21 connected to the connecting groove 24 is relatively balanced.

[0094] like Figure 5 As shown, the connecting groove 24 extends along a first direction, as indicated by the horizontal arrow, preferably the impeller radial direction; the individual air groove 21 extends along a second direction, as indicated by the vertical arrow, preferably the impeller axial direction; the angle between the first and second directions is 80°-100°, preferably 90°. Thus, the extension length of each air groove 21 along the second direction is approximately equal, and the air capacity of each air groove 21 is also relatively close, making the connecting groove 24 and the air groove 21 form a three-dimensional flow field structure. The connecting groove 24 can diffuse the gas to the transverse section between the first wall 10 and the second wall 30, while the air groove 21 can guide the gas from the second wall 30 to the depth direction of the first wall 10, forming a three-dimensional flow network of "transverse distribution - vertical penetration", which improves the uniformity of gas distribution in the air cavity. The gas can quickly diffuse into each air groove 21 and fill each air groove 21 in a similar time.

[0095] like Figure 6 As shown, the air guide plate 20 extends axially from the first wall 10 and is arc-shaped. When the aeration part 3 is installed on the container 1, it is preferable that the arc-shaped surface of the air guide plate 20 protrudes in a direction away from the center of the impeller 2. The air guide plate 20 includes a fixed end 27 fixed to the first wall 10 and a free end 28 away from the first wall 10. A second notch 23 is provided in the middle of the free end 28. A plurality of air guide plates 20 are arranged radially at intervals along the impeller 2, such that a plurality of second notches 23 are also arranged radially at intervals along the impeller 2, thereby forming a connecting groove 24 whose extension direction is approximately the same as the radial direction of the impeller 2.

[0096] like Figure 7 As shown, in this embodiment, the second wall 30 is provided with a positioning part 32 for positioning the free end 28 of the air guide plate 20. The positioning part 32 includes a positioning protrusion 321 and a limiting groove 322. The limiting groove 322 is located between adjacent positioning protrusions 321 in the radial direction of the impeller 2, such as... Figure 5As shown, the area of ​​the free end 28 of the air guide plate 20 without the first notch 22 engages with the corresponding limiting groove 322, and the air groove 21 is provided with the corresponding positioning protrusion 321. This confines the free end 28 of the air guide plate 20 within the limiting groove 322, preventing the air guide plate 20 from shifting or deviating, thus reducing the air guiding effect. Furthermore, since the air guide plate 20 extends from the first wall 10, and the positioning part 32 is located on the second wall 30, the engagement between the air guide plate 20 and the limiting groove 322 achieves indirect connection and positioning between the first wall 10 and the second wall 30, ensuring the overall structural stability of the aeration section 3. In other specific embodiments, the second wall 30 does not have a positioning part 32 for positioning the free end 28 of the air guide plate 20.

[0097] In this embodiment, the free end 28 of the air guide plate 20 can be interference-fitted with the limiting groove 322 to fix the first wall 10 and the second wall 30 together. The outer edges of the first wall 10 and the second wall 30 can also be fixed by welding or bonding to ensure the stability of the gas flow path in the air cavity 50.

[0098] like Figure 5 As shown, the length of the air guide plate 20 along the axial direction of the impeller 2 is L1, and the axial length of the air guide plate 20 extending to the inner end of the limiting groove 322 is L2, L2 / L1=0.1-0.6; the value of L2 / L1 reflects the balance between the usable volume of the air groove 21 and the limiting effect. If the value of L2 / L1 is too large, the limiting groove 322 will be too deep, the length of the air guide plate 20 inserted into the limiting groove 322 will be too long, the usable volume of the remaining air groove 21 will be too small, the positioning part 32 will occupy too much space in the air cavity 50, which will easily hinder the gas flow and reduce the space utilization rate. If the value of L2 / L1 is too small, the limiting groove 322 will be too shallow, the limiting effect on the air guide plate 20 will be poor, which will easily cause the air guide plate 20 to shift or deviate, reducing the air guiding effect.

[0099] like Figure 5 As shown, the thickness of the air guide plate 20 in the radial direction of the impeller 2 is d1, and the width of the air groove 21 in the radial direction of the impeller 2 is d2, where d1 / d2 = 0.5-3. The value of d1 / d2 reflects the balance between the structural strength of the air guide plate 20 and the usable volume of the air groove 21. If the value of d1 / d2 is too large, the radial width of the air groove 21 will be too small, increasing the difficulty for gas to enter the air groove 21 and easily hindering gas flow. If the value of d1 / d2 is too small, the air guide plate 20 will be too thin, resulting in poor support and little support for the space inside the air chamber 50.

[0100] Based on the aeration section 3 of this embodiment, such as Figures 24 to 27In the ninth embodiment shown, several aeration sections 3 are connected by a connecting plate 60 and are evenly arranged around the center of the impeller 2 to form a whole. Compared with a single aeration section 3, it has a larger volume and area. Therefore, when the container 1 vibrates due to the rotation of the impeller 2, each aeration section 3 can maintain a stable position. If the aeration section 3 is connected to the impeller base 201 through the connecting plate 60, the volume and stability of the impeller base 201 can be increased, thereby reducing the vibration of the impeller 2 itself and the vibration transmitted to the container 1. This not only ensures the gas output stability of the aeration section 3, but also ensures the relative positional stability of the impeller 2 and the aeration section 3, so that the impeller 2 can disperse the gas released by the aeration section 3 in time and improve the gas-liquid mixing efficiency. Specifically, the connecting disc 60 includes a first disc body 61 and a second disc body 62. The first walls 10 of several aeration sections 3 are fixedly connected to the first disc body 61 or are an integrated structure. The second walls 30 of several aeration sections 3 are fixedly connected to the second disc body 62 or are an integrated structure. The second disc body 62 is also provided with an air inlet corresponding to the air inlet 31 on the second wall 30. During installation, the first disc body 61 and the second disc body 62 are positioned opposite each other, and the first walls 10 and the second walls 30 of the aeration sections 3 are aligned, so that the air guide plate 20 on the first wall 10 and the positioning part 32 on the second wall 30 are correspondingly inserted until the position of the air guide plate 20 is restricted by the positioning part 32. The first disc body 61 and the second disc body 62 are fitted and fixed. The air guide plate 20 supports the first walls 10 and the second walls 30, improving the support effect on the air chamber 50 and ensuring the gas flow in the air chamber 50; it also ensures the installation stability and air supply stability of the aeration section 3. Preferably, in this embodiment, the first disc body 61 and the impeller chassis 201 are an integrated structure.

[0101] It should be noted that the aeration section 3 in other embodiments can be combined with Embodiment Nine to form a new combination structure of aeration section 3 and impeller 2.

[0102] Based on Example 9, such as Figure 1 As shown, container 1 is a bag. When the bag is fixed inside the rigid container 4 outside the bioreactor, the second wall 30 is attached to the inner wall of the rigid container 4, and the two ends of the gas guide plate 20 are respectively pressed against the first wall 10 and the second wall 30. Container 1 is located inside the rigid container 4 outside the bioreactor, and the second wall 30 is attached to the inner wall of the rigid container 4 so that the rigid container 4 provides mechanical support for the second wall 30, which helps to improve the fixing effect of the gas guide plate 20.

[0103] Preferably, in Embodiment 1 and Embodiment 9, the first wall 10, the second wall 30, and the air guide plate 20 are all made of rigid polymer, which has good compatibility and low assembly difficulty. The first disc 61 and the second disc 62 can also be made of rigid polymer.

[0104] Example 2

[0105] like Figure 8As shown, the difference between this embodiment 2 and embodiment 1 is that a baffle 40 is provided in the air cavity 50, and the air inlet 31 is located within the coverage area of ​​the baffle 40, that is, the air inlet 31 is located below the lower surface of the baffle 40. A diffuser 41 is provided between the air inlet 31 and the baffle 40 to allow gas to enter the air cavity 50 from the air inlet 31. An escape section 42 is provided between the diffuser 41 and the air groove 21 to allow gas to enter each air groove 21 from the diffuser 41. The free ends 28 of all the air guide plates 20 have a second notch 23 so that a fully connected connecting groove 24 is formed between all the air guide plates 20 and the second wall 30. The connecting groove 24 is located on both sides of the baffle 40. After the gas enters the diffuser 41 from the air inlet 31, it enters the connecting groove 24 through the escape section 42 and then diffuses to all the air grooves 21. At this time, the axial projection of the cross-connection part 25 of the connecting groove 24 and the air groove 21 does not coincide with the axial projection of the air inlet 31.

[0106] In this embodiment, the positioning part 32 may not be provided on the second wall 30. The air guide plate 20 extends from the first wall 10 to the second wall 30. After the outer edges of the first wall 10 and the second wall 30 are fixed by welding or bonding, the air guide plate 20 does not abut against the second wall 30 to maintain the communication groove 24. The baffle 40 can be bonded or welded to the free end 28 of the air guide plate 20 in advance to maintain a distance from the air inlet 31. The axial space between the lower surface of the baffle 40 and the air inlet 31 forms a diffusion part 41, and the first gap 421 between the lower surface of the baffle 40 and the second wall 30 forms an escape part 42. The function of the baffle 40 is to further disperse the gas entering from the air inlet 31. After the gas enters the diffusion part 41, it can diffuse for the first time, and then hit the baffle 40 and enter each gas groove 21 through the escape part 42, so as to avoid the gas from being concentrated near the air inlet 31 and escaping in a concentrated manner, which is beneficial to improving the dispersion of the gas escape.

[0107] Example 3

[0108] The difference between this embodiment and Embodiment 2 is that, as Figures 9 to 13As shown, at least a portion of the air guide plate 20 has a first notch 22, and several first notches 22 form a receiving cavity 26 for receiving the baffle 40. A second notch 23 is located within the first notch 22. When the baffle 40 is located in the receiving cavity 26, the connecting groove 24 is located axially above the baffle 40. The air inlet 31 is located within the coverage area of ​​the baffle 40, that is, the air inlet 31 is located below the lower surface of the baffle 40. In this specific embodiment, the second wall 30 is provided with a positioning point for the free end 28 of the air guide plate 20 and a positioning point for the baffle 40. Part 32, a second gap 423 is provided between the positioning part 32 and the baffle 40, the second gap 423 forms a partial escaping part 42, the positioning part 32 is spaced apart from the air inlet 31, so that the first gap 421 between the lower surface of the baffle 40 and the second wall 30 forms another partial escaping part 42; thus, after the gas enters the diffusion part 41 through the air inlet 31, it enters the partial air groove 21 through the escaping part 42, and then rises from the inside of the partial air groove 21 into the connecting groove 24, and then diffuses to all air grooves 21.

[0109] The positioning part 32 includes a positioning protrusion 321 and a limiting groove 322. The limiting groove 322 is located between adjacent positioning protrusions 321 in the radial direction of the impeller 2. The area of ​​the free end 28 of the air guide plate 20 without the first notch 22 is engaged with the limiting groove 322. The air groove 21 is provided corresponding to the positioning protrusion 321. The limiting groove 322 is used to position the air guide plate 20, preventing the air guide plate 20 from shifting or deviating and reducing the air guiding effect. Moreover, since the air guide plate 20 extends from the first wall 10 and the positioning part 32 is located on the second wall 30, the engagement between the air guide plate 20 and the limiting groove 322 achieves indirect connection and positioning between the first wall 10 and the second wall 30, ensuring the overall structural stability of the aeration part 3.

[0110] like Figure 11 and Figure 12 As shown, the positioning part 32 also includes a positioning groove 323, and the baffle 40 is provided with a positioning member 43 protruding from the lower surface of the baffle 40. The positioning member 43 is engaged with the positioning groove 323. In this embodiment, as shown... Figure 12 and 13As shown, the positioning element 43 is located on the lower surface of the baffle 40. Partially positioned protrusions 321 are located below the lower surface of the baffle 40 and spaced upwards around the impeller, forming positioning grooves 323. The centerline of the positioning grooves 323 passes through the center of the air inlet 31. Simultaneously, the air inlet 31 is located below the lower surface of the baffle 40. The positioning protrusions 321 are closer to the air inlet 31. When the baffle 40 is impacted by the gas from the air inlet 31, the distance between the force point and the positioning point is shorter, and the lever arm is smaller. This allows the load of the baffle 40 to be directly transferred to the positioning protrusions 321 and the second wall 30, ensuring the stability of the baffle 40. Furthermore, the radial length of the positioning grooves 323 is equal to the radial length of the baffle 40. Using positioning grooves 323 of equal length to the baffle 40 to position the baffle 40 increases the contact area between the positioning grooves 323 and the baffle 40, resulting in better positioning and ensuring the stability of the baffle 40 under the action of the airflow from the air inlet 31. In other embodiments, the second wall 30 does not have a positioning part 32 for positioning the free end 28 of the air guide plate 20.

[0111] Preferably, in this embodiment, the projection of the baffle 40 and the projection of the air outlet 12 are misaligned along the axial direction of the impeller 2. Therefore, in Figure 13 The cross-sectional schematic diagram does not show the air vents. The positional relationship between the baffle 40 and the air outlet 12 is as follows: Figure 4 As shown, Figure 4 The dashed line in the figure represents baffle 40; in this embodiment, as Figure 9 As shown, the baffle 40 corresponds to the air inlet 31 and the connecting groove 24. To prevent gas from bypassing the baffle 40 and entering the connecting groove 24, it flows directly out from the air outlet 12 in the air groove 21 area along the axis of the baffle 40. By misaligning the projection of the baffle 40 with the projection of the air outlet 12, the gas entering from the air inlet 31 hits the baffle 40 and moves to the side. Some of the gas directly enters the area on both sides of the baffle 40 in the air groove 21 and is discharged from the air outlet 12 in that area. Some of the gas bypasses the baffle 40 and enters the connecting groove 24 and then enters each air groove 21. Since there is no air outlet 12 in the area corresponding to the air groove 21 and the connecting groove 24, the gas is forced to move to the area outside the baffle 40 in the air groove 21 and then be discharged from the corresponding air outlet 12. This promotes the uniformity of gas distribution and escape in the air groove 21.

[0112] Example 4

[0113] like Figure 14As shown, the difference between this embodiment and Embodiment 3 is that the sidewall of the receiving cavity 26 and the side of the baffle 40 have a first gap 422, which forms a partial escaping portion 42 to connect the diffusion portion 41 and all the gas slots 21; at the same time, there is a second gap 423 between the partial positioning portion 32 and the baffle 40, which forms a partial escaping portion 42. The positioning portion 32 and the air inlet 31 are spaced apart, so that the first gap 421 between the lower surface of the baffle 40 and the second wall 30 forms another part of the escaping portion 42; the three together form the escaping portion 42. Thus, after the gas enters the diffusion portion 41 from the air inlet 31, it hits the baffle 40, enters the escaping portion 42, and bypasses the baffle 40. Some of the gas directly enters the gas slots 21, and some of the gas enters the connecting slot 24 and diffuses to all the gas slots 21.

[0114] In other specific embodiments, the sidewall of the receiving cavity 26 and the side of the baffle 40 also have a first gap 422. The first gap 422 forms a partial escaping portion 42 to connect the diffuser portion 41 and all the gas slots 21. At the same time, a partial positioning portion 32 is located on the lower surface of the baffle 40. Although it does not form a second gap with the baffle 40, the positioning portion 32 will not interfere with the normal flow of gas. Meanwhile, the first gap 421 between the lower surface of the baffle 40 and the second wall 30 forms another part of the escaping portion 42. That is, the first gap 421 and the first gap 422 together constitute the escaping portion 42. Thus, after the gas enters the diffuser portion 41 through the air inlet 31, it hits the baffle 40, escapes in the escaping portion 42 and bypasses the positioning portion 32 and the baffle 40. Some of the gas directly enters the gas slots 21, and some of the gas enters the connecting slot 24 and diffuses to all the gas slots 21.

[0115] Example 5

[0116] like Figure 15 and Figure 16 As shown, the difference between this embodiment and Embodiment 3 is that the second wall 30 is provided with a positioning part 32 of the positioning baffle 40. The positioning part 32 includes a positioning groove 323. The baffle 40 is provided with a positioning member 43 protruding from the lower surface of the baffle 40. The positioning member 43 is engaged with the positioning groove 323. The center line of the positioning groove 323 passes through the center of the air inlet 31. At the same time, the air inlet 31 is located below the lower surface of the baffle 40. The positioning protrusion 321 is relatively closer to the air inlet 31. During impact, the distance between the stress point and the positioning point is shorter, resulting in less force. This allows the load of the baffle 40 to be directly transferred to the positioning protrusion 321 and the second wall 30, ensuring the stability of the baffle 40. Furthermore, the radial length of the positioning groove 323 is equal to the radial length of the baffle 40. By using the positioning groove 323, which is the same length as the baffle 40, to position the baffle 40, the contact area between the positioning groove 323 and the baffle 40 is increased, resulting in a better positioning effect and ensuring the stability of the baffle 40 under the airflow of the air inlet 31.

[0117] In this embodiment, the positioning part 32 is not spaced apart from the air inlet 31. There is a second gap 423 between the positioning part 32 and the baffle 40. The second gap 423 forms a dissipation part 42. After the gas enters the diffusion part 41 through the air inlet 31, it enters part of the air groove 21 through the dissipation part 42, and then enters the connecting groove 24 from part of the air groove 21, and then diffuses to all the air grooves 21.

[0118] In this embodiment, when assembling the aeration section 3, the positioning member 43 of the baffle 40 can be first engaged with the positioning groove 323. The positioning member 43 can be interference-fitted with the positioning groove 323. Then, the first wall 10 and the second wall 30 are joined together. The air guide plate 20 extends from the first wall 10 to the second wall 30. After the outer edges of the first wall 10 and the second wall 30 are fixed by welding or bonding, the air guide plate 20 abuts against the second wall 30 to support the air chamber 50.

[0119] Example 6

[0120] like Figure 17 and Figure 18 As shown, the difference between this embodiment and Embodiment 3 is that the second wall 30 is provided with a positioning part 32 for the positioning baffle 40. The positioning part 32 includes a positioning groove 323. The baffle 40 is provided with a positioning member 43 protruding from the lower surface of the baffle 40. The positioning member 43 is engaged with the positioning groove 323. The radial length of the positioning groove 323 is equal to the radial length of the baffle 40. By using the positioning groove 323, which is the same length as the baffle 40, the baffle 40 is positioned, which increases the contact area between the positioning groove 323 and the baffle 40, resulting in a better positioning effect and ensuring the stability of the baffle 40 under the airflow of the air inlet 31. In this embodiment, the positioning part 32 is spaced apart from the air inlet 31, and there is a second gap 423 between the positioning part 32 and the baffle 40. The second gap 423 forms a partial diffuser 42. The positioning part 32 is spaced apart from the air inlet 31, so that the first gap 421 between the lower surface of the baffle 40 and the second wall 30 forms another part diffuser 42. After the gas enters the diffuser 41 through the air inlet 31, it enters a partial gas groove 21 through the diffuser 42, and then enters the connecting groove 24 from the partial gas groove 21, and then diffuses to all gas grooves 21.

[0121] In this embodiment, when assembling the aeration section 3, the positioning member 43 of the baffle 40 can be first engaged with the positioning groove 323. The positioning member 43 can be interference-fitted with the positioning groove 323. Then, the first wall 10 and the second wall 30 are joined together. The air guide plate 20 extends from the first wall 10 to the second wall 30. After the outer edges of the first wall 10 and the second wall 30 are fixed by welding or bonding, the air guide plate 20 abuts against the second wall 30 to support the air chamber 50.

[0122] Example 7

[0123] like Figure 19 and Figure 20As shown, the difference between this embodiment and Embodiment 3 is that the sidewall of the receiving cavity 26 and the side of the baffle 40 have a first gap 422, which forms a partial escaping portion 42 to connect the diffuser portion 41 and all the air slots 21; at the same time, the positioning member 43 of the baffle 40 is located at both ends of the baffle 40 along the impeller radial direction, and the positioning groove 323 is also located on the second wall 30 in the area corresponding to both ends of the baffle 40 along the impeller radial direction; the positioning portion 32 does not extend below the lower surface of the baffle 40. The first gap 421 between the lower surface and the second wall 30 forms a partial escaping section 42. The first gap 422 between the side wall of the receiving cavity 26 and the side of the baffle 40 forms another partial escaping section 42. At the same time, after the gas enters the diffuser section 41 through the air inlet 31, part of it directly hits the baffle 40, enters the escaping section 42 and bypasses the baffle 40, and part of it directly passes through the escaping section 42. Then, part of the gas directly enters the gas groove 21, and part of the gas enters the connecting groove 24 and diffuses to all the gas grooves 21.

[0124] In this embodiment, since the positioning groove 323 is relatively short and the contact area between the positioning member 43 and the positioning groove 323 is small, when assembling the aeration section 3, the positioning member 43 of the baffle 40 can be first snapped into the positioning groove 323, and then the connection strength between the positioning member 43 and the positioning groove 323 can be further strengthened by welding or bonding to ensure the stability of the baffle 40 under the action of the airflow at the air inlet 31. Then, the first wall 10 and the second wall 30 are joined together, and the air guide plate 20 extends from the first wall 10 to the second wall 30 and is inserted into the limiting groove 322 accordingly. The air guide plate 20 and the limiting groove 322 can be interference-fitted. The engagement of the air guide plate 20 and the limiting groove 322 realizes the indirect connection and positioning of the first wall 10 and the second wall 30, ensuring the overall structural stability of the aeration section 3. After the first wall 10 and the second wall 30 are fixed, the air guide plate 20 abuts against the second wall 30 to support the air chamber 50.

[0125] Example 8

[0126] like Figures 21 to 23 As shown, the difference between this embodiment and embodiment two is that the air inlet 31 and the baffle 40 are misaligned, the diffuser 41 is located on the side of the baffle 40, the baffle 40 is attached and fixed to the first wall 10 and spaced apart from the second wall 30 to form the escaping part 42, so as to connect the air inlet 31 and each air groove 21.

[0127] Specifically, such as Figure 22 As shown, the air guide plate 20 extends from the first wall 10, and several air guide plates 20 are arranged parallel to each other in a direction perpendicular to the radial direction of the impeller 2. Adjacent air guide plates 20 have air grooves 21 between them. Each air guide plate 20 has a second notch 23 on one side radially in front of the impeller 2, and the other side is connected to the outer edge of the first wall 10. Several second notches 23 form a connecting groove 24 on one side of the air guide plate 20. Figure 21 and Figure 23 As shown, the air inlet 31 is located on the second wall 30 in the area of ​​the first wall 10 where the air guide plate 20 is not provided. The baffle 40 is disposed between the air inlet 31 and the air guide plate 20 in the radial direction. The baffle 40 is fixedly disposed with the first wall 10 and spaced apart from the second wall 30, having a first gap 421 to form an escaping portion 42. A positioning portion 32 is provided on the area of ​​the second wall 30 corresponding to the air guide plate 20. The positioning portion 32 includes a positioning protrusion 321 and a limiting groove 322. The limiting groove 322 is located on the positioning protrusion 321 that is radially adjacent to the impeller 2. Between them, the area of ​​the free end 28 of the air guide plate 20 without the second notch 23 is engaged with the corresponding limiting groove 322, and the air groove 21 is set with the corresponding positioning protrusion 321. The limiting groove 322 is used to position the air guide plate 20 to prevent the air guide plate 20 from shifting or deviating and reducing the air guiding effect. Moreover, since the air guide plate 20 extends from the first wall 10 and the positioning part 32 is set on the second wall 30, the engagement between the air guide plate 20 and the limiting groove 322 realizes the indirect connection and positioning between the first wall 10 and the second wall 30, ensuring the overall structural stability of the aeration part 3.

[0128] The above embodiments are merely preferred embodiments of this utility model and should not be construed as limiting the scope of protection of this utility model. Any non-substantial changes and substitutions made by those skilled in the art based on this utility model shall fall within the scope of protection claimed by this utility model.

Claims

1. A bioreactor, comprising a container, an aeration section, and an impeller disposed within the container, wherein the aeration section is configured such that at least its outlet surface is located within the container, characterized in that: The aeration section is distributed circumferentially around the impeller. The aeration section includes a first wall and a second wall arranged opposite to each other, an air cavity located between the first wall and the second wall, and an air inlet communicating with the air cavity. The surface of the first wall facing the impeller is the air outlet surface, and the air inlet is located on the second wall. The air chamber is provided with a plurality of spaced air guide plates. The air guide plates extend between the first wall and the second wall along the gas dispersion direction, and air grooves are formed between adjacent air guide plates. Several of the air grooves are connected to the air inlet. The air guide plate has a second notch, and a plurality of the second notches form a connecting groove. The connecting groove intersects with and connects with a plurality of the air grooves to form a cross-connecting part. The first wall has multiple air outlets in the area corresponding to the air groove that communicate with the air cavity.

2. The bioreactor as described in claim 1, characterized in that, The axial projection of the cross-connection portion coincides with the axial projection of the air inlet.

3. The bioreactor as described in claim 1, characterized in that, The connecting groove extends along a first direction, and the individual air groove extends along a second direction, with the angle between the first direction and the second direction being 80°-100°.

4. The bioreactor as described in claim 1, characterized in that, At least one baffle is provided inside the air chamber. A diffuser is provided between the air inlet and the baffle to allow gas to enter the air chamber through the air inlet. An escape section is provided between the diffuser and the air slot to allow gas to enter each of the air slots through the diffuser.

5. The bioreactor as described in claim 4, characterized in that, The baffle is clearance-fitted with the second wall and the air inlet. The axial projection of the baffle covers the air inlet. The baffle includes a lower surface facing the opening, an upper surface axially opposite to the lower surface, and a side surface connecting the upper surface and the lower surface. The axial space between the lower surface of the baffle and the air inlet forms the diffuser portion. The first gap between the lower surface of the baffle and the second wall forms at least a portion of the escaping portion.

6. The bioreactor as described in claim 4, characterized in that, At least a portion of the air guide plate has a first notch, and a plurality of the first notches form a receiving cavity for accommodating the baffle.

7. The bioreactor as described in claim 6, characterized in that, The sidewall of the receiving cavity and the side of the baffle have a first gap, the first gap forming at least part of the escaping portion to connect the diffuser portion and all the air slots.

8. The bioreactor as described in claim 5, 6, or 7, characterized in that, The air guide plate includes a fixed end fixed to the first wall and a free end away from the first wall. The second wall is provided with a positioning part for positioning the free end of the air guide plate and / or the baffle. At least a portion of the positioning part and the baffle have a second gap, and the second gap forms at least a portion of the escaping part.

9. The bioreactor as described in claim 8, characterized in that, The positioning part is spaced apart from the air inlet, and the first gap between the lower surface of the baffle and the second wall around the air inlet forms part of the escaping part; At least a portion of the positioning portion is located on the lower surface of the baffle and is clearance-fitted with the lower surface of the baffle to form a portion of the escaping portion.

10. The bioreactor as described in claim 4, characterized in that, The air inlet is offset from the baffle, the diffuser is located on the side of the baffle, and the baffle is spaced apart from the first wall or the second wall to form the escaping portion, so as to connect the air inlet and each of the air slots.

11. The bioreactor as described in claim 8, characterized in that, The positioning part includes a positioning protrusion and a limiting groove. The limiting groove is located between adjacent positioning protrusions in the radial direction of the impeller. The area of ​​the free end of the air guide plate without the first notch is engaged with the limiting groove. The air groove is provided corresponding to the positioning protrusion.

12. The bioreactor as described in claim 8, characterized in that, The positioning part includes a positioning groove, and the baffle is provided with a positioning element protruding from the lower surface of the baffle, the positioning element being engaged with the positioning groove.

13. The bioreactor as described in claim 12, characterized in that, The positioning element is located on the lower surface of the baffle. The positioning part further includes positioning protrusions, at least a portion of which are located below the lower surface of the baffle and spaced apart circumferentially on the impeller to form the positioning grooves; or... The positioning element is located at both ends of the baffle along the radial direction of the impeller, and the positioning groove is also located on the second wall in the region corresponding to both ends of the baffle along the radial direction of the impeller.

14. The bioreactor as described in claim 11, characterized in that, The length of the air guide plate along the impeller axis is L1, and the axial length of the air guide plate extending to the inner end of the limiting groove is L2, where L2 / L1 = 0.1-0.6; and / or, The thickness of the air guide plate in the radial direction of the impeller is d1, and the width of the air groove in the radial direction of the impeller is d2, where d1 / d2 = 0.5-3.

15. The bioreactor as described in claim 4, characterized in that, Along the axial direction of the impeller, the projection of the baffle is misaligned with the projection of the air outlet.

16. The bioreactor according to any one of claims 1 to 7, characterized in that, The aeration section has multiple air outlets radially distributed around the center of the air inlet; the aeration sections are radially distributed around the center of the impeller.

17. The bioreactor as described in claim 16, characterized in that, Several of the aforementioned aeration units are connected by a connecting disc.

18. The bioreactor according to any one of claims 1 to 7, characterized in that: The first wall, the second wall, and the air guide plate are all made of rigid polymer.

19. The bioreactor according to any one of claims 1 to 7, characterized in that, The container is a bag. When the bag is fixed in a rigid container outside the bioreactor, the second wall is attached to the inner wall of the rigid container, and the two ends of the air guide plate are respectively pressed against the first wall and the second wall.

Images