Reaction device for cell culture

Abstract

The utility model provides a reaction device for cell culture. The reaction device for cell culture comprises a culture tank one, a culture tank two, a display component one and a display component two, further comprises a bottom plate, a support, a pump body one, a pump body two and an electrical cabinet, the bottom plate is flat and the culture tank one, the culture tank two and the electrical cabinet are sequentially arranged on the bottom plate, the bottom of the support is vertically fixed on the bottom plate, the side of one end of the support is fixed on the side of the electrical cabinet, the display component one and the display component two are fixed on the support and the display component one is adjacent to the culture tank one, the display component two is adjacent to the culture tank two, the pump body one is fixed on the culture tank one, the pump body two is fixed on the culture tank two, further comprises a pipeline unit, the pump body one can convey the fluid medium in the culture tank one to the culture tank two through the pipeline unit, the pump body two can discharge the fluid medium in the culture tank two through the pipeline unit. The reaction device for cell culture is compact in structure.

Description

Technical Field

[0001] This utility model relates to the field of mechanical technology, and more specifically to a reaction device for cell culture. Background Technology

[0002] In cell culture (especially in industrial-grade biopharmaceutical production), the process of gradually scaling up from a "small tank" to a "large tank" is called scale-up or seed train.

[0003] This strategy of "starting small and gradually scaling up" is the safest, most efficient, and controllable method, proven through long-term practice. Its core objectives include: 1. Ensure cells are in optimal growth condition. Small culture tanks have a small volume, making it easier to precisely control parameters such as temperature, pH, dissolved oxygen, and stirring.

[0004] Cells proliferate rapidly in an ideal environment, forming healthy, highly active "seed cells".

[0005] 2. Reduce pollution risk Small-scale operations involve fewer steps and are easier to control aseptically.

[0006] Each stage of amplification can undergo quality testing (such as sterility checks, cell viability, and gene stability) to ensure it is "qualified" before proceeding to the next stage.

[0007] 3. Achieve controllable large-scale production Similar to a snowball effect: from a few milliliters → hundreds of milliliters → a few liters → hundreds of liters → tens of thousands of liters.

[0008] The number of cells in each stage is sufficient to efficiently "inoculate" the next stage of the large container, avoiding the "dilution effect".

[0009] 4. Improve production success rate and economic efficiency. Small-scale production is low-cost and allows for rapid process adjustments.

[0010] Large tanks are only used when the cells are in a stable state and in sufficient quantity, maximizing the use of expensive equipment and culture media.

[0011] In current cell culture processes, small and large culture tanks are typically two separate vessels. Each tank has its own independent equipment, such as displays for parameters like temperature, pH, and dissolved oxygen. In other words, the small culture tank and its equipment form one operational unit, while the large culture tank and its equipment form another independent operational unit.

[0012] The two work units make the work environment complex, and the entire cell culture work environment appears messy. Utility Model Content

[0013] The purpose of this invention is to address the aforementioned problems in existing technologies by providing a compact reaction device for cell culture.

[0014] To achieve the above objectives, this utility model can be implemented through the following technical solutions: A reaction apparatus for cell culture includes a culture tank 1, a culture tank 2, a display component 1, and a display component 2. It is characterized by further including a base plate, a support, a pump body 1, a pump body 2, and an electrical box. The base plate is flat, and the culture tank 1, culture tank 2, and electrical box are arranged adjacent to each other on the base plate. The bottom of the support is vertically fixed to the base plate, and one side of the support is fixed to the side of the electrical box. The display component 1 and display component 2 are both fixed to the support, with display component 1 adjacent to culture tank 1 and display component 2 adjacent to culture tank 2. Pump body 1 is fixed to culture tank 1, and pump body 2 is fixed to culture tank 2. The apparatus also includes a piping unit, where pump body 1 can transport fluid media from culture tank 1 to culture tank 2 through the piping unit, and pump body 2 can discharge fluid media from culture tank 2 through the piping unit.

[0015] In the aforementioned reaction apparatus for cell culture, the upper ends of the electrical box, the upper ends of display component one, and the upper ends of display component two are all close to the upper end of the support.

[0016] In the above-mentioned reaction apparatus for cell culture, a support platform 1 and a support platform 2 are fixedly connected to the base plate. The height of the support platform 1 is greater than the height of the support platform 2. The culture tank 1 is fixedly connected to the support platform 1, and the culture tank 2 is fixedly connected to the support platform 2.

[0017] In the above-mentioned reaction apparatus for cell culture, the lower part of the culture vessel two has a rod-shaped connecting rod, the middle part of which is designed to pass through the support platform two, and the lower end of the connecting rod abuts against the bottom plate.

[0018] In the aforementioned reaction apparatus for cell culture, a flexible foot pad is detachably connected to the lower end of the connecting rod.

[0019] In the aforementioned reaction apparatus for cell culture, a baffle is threaded onto the connecting rod, and the baffle can abut against the upper part of the support platform.

[0020] The aforementioned connecting rod, baffle, and foot pad form a foot unit, and there are several foot units, which are circumferentially distributed on the outside of the second culture tank.

[0021] In the above-mentioned reaction apparatus for cell culture, the pipeline unit includes a tube body one, a tube body two, a tube body three, a valve one, a valve two, and a pressure vessel. The two ends of the tube body one are respectively connected to a pump body one and a pressure vessel. The two ends of the tube body two are respectively connected to a pump body two and a pressure vessel. One end of the tube body three is connected to the pressure vessel, and the other end of the tube body three is open to the outside. The valve one is installed on the tube body one, and the valve two is installed on the tube body three.

[0022] In the aforementioned reaction apparatus for cell culture, the pressure vessel is equipped with a pressure gauge that displays its internal pressure value.

[0023] In the aforementioned reaction apparatus for cell culture, tube two, tube three, and the pressure vessel are located between the support and the support platform two.

[0024] Compared with existing technologies, this reaction apparatus for cell culture has a more compact structure because culture tank one, culture tank two, and the electrical box are all located on the base plate. Furthermore, the support frame connects to display components one and two, as well as the electrical box, thus improving the overall compactness and connection stability of the apparatus and making it highly valuable for practical use.

[0025] Meanwhile, the adjustable height of the second culture tank appropriately improves the applicability of the entire device, and most of the components of the piping unit are located between the second support platform and the bracket, which further improves the structural compactness of the entire device. Attached Figure Description

[0026] Figure 1 This is a three-dimensional structural diagram of the reaction apparatus used for cell culture.

[0027] Figure 2 yes Figure 1 A schematic diagram of the partial structure at part A in the middle.

[0028] In the picture: 1. Culture tank one; 2. Culture tank two; 3. Display component one; 4. Display component two; 5. Base plate; 6. Support; 7. Pump body one; 8. Pump body two; 9. Electrical box; 10. Support platform one; 11. Support platform two; 12. Connecting rod; 13. Baffle plate; 14. Foot pad; 15. Pipe body one; 16. Pipe body two; 17. Pipe body three; 18. Valve one; 19. Valve two; 20. Pressure tank; 21. Pressure gauge. Detailed Implementation

[0029] The following are specific embodiments of the present invention, and the technical solution of the present invention will be further described in conjunction with the accompanying drawings.

[0030] like Figure 1-2As shown, the reaction apparatus for cell culture includes a culture tank 1, a culture tank 2, a display component 3, and a display component 4. It also includes a base plate 5, a support 6, a pump 7, a pump 8, and an electrical box 9. The base plate 5 is flat, and the culture tank 1, culture tank 2, and electrical box 9 are arranged adjacent to each other on the base plate 5. The bottom of the support 6 is vertically fixed to the base plate 5, and one side of the support 6 is fixed to the side of the electrical box 9. The display components 3 and 4 are both fixed to the support 6, with display component 3 adjacent to culture tank 1 and display component 4 adjacent to culture tank 2. The pump 7 is fixed to culture tank 1, and the pump 8 is fixed to culture tank 2. The apparatus also includes a piping unit. Pump 7 can transport the fluid medium in culture tank 1 to culture tank 2 through the piping unit, and pump 8 can discharge the fluid medium in culture tank 2 through the piping unit.

[0031] Culture jar 1 is a small culture jar, and culture jar 2 is a large culture jar.

[0032] Because culture tank 1 is small, it allows for more precise control of parameters such as temperature, pH, and dissolved oxygen within the culture tank 1.

[0033] Cells proliferate rapidly in an ideal environment, forming healthy, highly active "seed cells".

[0034] Culture tank 2 is only activated when the cells are in a stable state and in sufficient quantity, maximizing the use of expensive equipment and culture medium. That is, after the cells in culture tank 1 reach the set requirements, the fluid medium in culture tank 1 is introduced into culture tank 2 through the pipeline unit via pump 7.

[0035] Once the cells in culture tank 2 have reached the set requirements, pump 2 will pump the fluid medium from culture tank 2 through the piping unit to output it to the device. Of course, a corresponding storage container is provided at the output point to collect the cultured medium.

[0036] Display components 3 and 4 are existing technologies, which can display parameters such as temperature, pH, and dissolved oxygen inside the corresponding culture tank. Since display components 3 and 4 are commercially available parts, the specific technical solutions of the display components will not be described in detail in this embodiment.

[0037] The electrical box 9 contains electrical equipment for controlling the operation of pump body 7 and pump body 8. Since the electrical box 9 is also a commercially available component, the specific technical details of the electrical box 9 will not be elaborated upon in this embodiment.

[0038] The upper ends of the electrical box 9, the display component 1 3, and the display component 2 4 are all close to the upper end of the bracket 6.

[0039] This structure allows for maintaining an appropriate distance between display component 3 and culture tank 1, and between display component 4 and culture tank 2.

[0040] At the same time, since the side of the electrical box 9 is connected to the bracket 6 over a large area, this can also improve the stability of the bracket 6 and prevent the bracket 6 from tipping over.

[0041] Support platform 10 and support platform 2 are fixedly connected to the base plate 5. The height of support platform 10 is greater than that of support platform 2. Culture tank 1 is fixedly connected to support platform 10, and culture tank 2 is fixedly connected to support platform 2.

[0042] Because the culture tank 1 is relatively small, the support platform 10 is relatively tall, so as not to affect the strength of the entire device.

[0043] Conversely, since the culture tank 2 is relatively large, the support platform 211 has a relatively low height to avoid affecting the overall strength of the device.

[0044] The lower part of the culture tank 2 has a rod-shaped connecting rod 12, the middle part of which is designed to pass through the support platform 2 11, and the lower end of the connecting rod 12 abuts against the bottom plate 5.

[0045] This structure enables the connecting rod 12 to provide stable support for the culture tank 2.

[0046] The lower end of the connecting rod 12 is detachably connected to a flexible foot pad 14.

[0047] The foot pad 14 is made of rubber. The flexible foot pad 14 can prevent the lower end of the connecting rod 12 from making rigid contact with the base plate 5.

[0048] A baffle plate 13 is threaded onto the connecting rod 12, and the baffle plate 13 can abut against the upper part of the support platform 11.

[0049] When it is necessary to adjust the initial height position of culture tank 2, rotate baffle 13 to the set position. Since baffle 13 abuts against the upper part of support platform 2 11, the lower end of connecting rod 12 is suspended in the air after the above operation.

[0050] At this point, the original foot pad 14 is removed and replaced with a foot pad 14 that is either higher or lower in height. After the baffle 13 is removed, the change in the height of the foot pad 14 ultimately alters the initial height of the culture tank 2, thus adapting it to the corresponding operating conditions.

[0051] The foot pad 14 has a recessed notch, and the lower end of the connecting rod 12 matches the notch. This structure allows for quick assembly and disassembly between the foot pad 14 and the connecting rod 12.

[0052] The aforementioned connecting rod 12, baffle 13 and foot pad 14 form a foot unit, and there are several foot units, which are circumferentially distributed on the outside of the culture tank 2.

[0053] Multiple support units can provide stable support for culture tank 2.

[0054] The piping unit includes pipe body 15, pipe body 2 16, pipe body 3 17, valve 1 18, valve 2 19, and pressure tank 20. The two ends of pipe body 15 are connected to pump body 1 7 and pressure tank 20, respectively. The two ends of pipe body 2 16 are connected to pump body 2 8 and pressure tank 20, respectively. One end of pipe body 3 17 is connected to pressure tank 20, and the other end of pipe body 3 17 is open to the outside. Valve 1 18 is installed on pipe body 15, and valve 2 19 is installed on pipe body 3 17.

[0055] When valve two is closed and valve one is open, pump body one can stably deliver the fluid medium from culture tank two into culture tank two during operation. During this process, the fluid medium will pass through pump body two when it is not in operation.

[0056] When valve one is closed and valve two is opened, the fluid medium inside culture tank two can be stably output through pipe three under the action of pump body two.

[0057] Since the initial height of culture vessel two will change, tube two is a flexible culture vessel, which can adapt to changes in the height of the culture vessel.

[0058] The pressure tank 20 has a pressure gauge 21 that can display its internal pressure value.

[0059] A pressure gauge provides a direct and timely understanding of the pressure values ​​within the piping unit.

[0060] The second pipe body 16, the third pipe body 17, and the pressure tank 20 are located between the bracket 6 and the second support platform 11.

[0061] This structure allows the piping units to make the most of the gap between the brackets and the support platform, effectively improving the overall structural compactness of the device.

[0062] This reaction apparatus for cell culture has a compact structure because culture tank 1, culture tank 2, and the electrical box are all located on the base plate. Furthermore, the support frame connects to display components 1 and 2, as well as the electrical box, thus improving the overall compactness and connection stability of the apparatus and giving it high practical value.

[0063] Meanwhile, the adjustable height of the second culture tank appropriately improves the applicability of the entire device, and most of the components of the piping unit are located between the second support platform and the bracket, which further improves the structural compactness of the entire device.

[0064] The above-described technical solution of this utility model addresses the problem that existing technical solutions are too simplistic and provides a solution that is significantly different from existing technologies. The parts not covered in this application's technical solution are the same as or can be implemented using existing technologies, and will not be described in detail here.

[0065] The technical solutions in the above embodiments have clearly and completely described the content of this utility model. Obviously, the described embodiments are only some embodiments of this utility model, and not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of this utility model.

Claims

1. A reaction apparatus for cell culture, comprising a culture vessel one, a culture vessel two, a display component one, and a display component two, characterized in that, It also includes a base plate, a support, a first pump body, a second pump body, and an electrical box. The base plate is flat, and the first culture tank, the second culture tank, and the electrical box are arranged adjacent to each other on the base plate. The bottom of the support is vertically fixed to the base plate, and the side of one end of the support is fixed to the side of the electrical box. The first display component and the second display component are both fixed to the support, with the first display component adjacent to the first culture tank and the second display component adjacent to the second culture tank. The first pump body is fixed to the first culture tank, and the second pump body is fixed to the second culture tank. It also includes a pipeline unit. The first pump body can transport the fluid medium in the first culture tank to the second culture tank through the pipeline unit, and the second pump body can discharge the fluid medium in the second culture tank through the pipeline unit.

2. The reaction apparatus for cell culture according to claim 1, characterized in that, The upper ends of the electrical box, the first display component, and the second display component are all close to the upper end of the bracket.

3. The reaction apparatus for cell culture according to claim 2, characterized in that, Support platform 1 and support platform 2 are fixedly connected to the base plate. The height of support platform 1 is greater than the height of support platform 2. Culture tank 1 is fixedly connected to support platform 1, and culture tank 2 is fixedly connected to support platform 2.

4. The reaction apparatus for cell culture according to claim 3, characterized in that, The lower part of the second culture tank has a rod-shaped connecting rod, the middle of which is designed to pass through the second support platform, and the lower end of the connecting rod abuts against the bottom plate.

5. The reaction apparatus for cell culture according to claim 4, characterized in that, The lower end of the connecting rod is detachably connected to a flexible foot pad.

6. The reaction apparatus for cell culture according to claim 5, characterized in that, A baffle is threaded onto the connecting rod, and the baffle can abut against the upper part of the second support platform.

7. The reaction apparatus for cell culture according to claim 6, characterized in that, The aforementioned connecting rod, baffle, and foot pad form a foot unit, and there are several foot units, which are circumferentially distributed on the outside of the second culture tank.

8. The reaction apparatus for cell culture according to claim 7, characterized in that, The pipeline unit includes a pipe body 1, a pipe body 2, a pipe body 3, a valve 1, a valve 2, and a pressure tank. The two ends of the pipe body 1 are connected to the pump body 1 and the pressure tank, respectively. The two ends of the pipe body 2 are connected to the pump body 2 and the pressure tank, respectively. One end of the pipe body 3 is connected to the pressure tank, and the other end of the pipe body 3 is open to the outside. The valve 1 is installed on the pipe body 1, and the valve 2 is installed on the pipe body 3.

9. The reaction apparatus for cell culture according to claim 8, characterized in that, The pressure tank has a pressure gauge that displays its internal pressure value.

10. The reaction apparatus for cell culture according to claim 9, characterized in that, The second and third pipes and the pressure tank are located between the bracket and the second support platform.

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