Electrophoretic channel bubble expelling device

Abstract

The utility model discloses an electrophoretic passage bubble discharging device, include: electrophoretic passage anode end, electrophoretic passage cathode end, glue injection pump and electrophoretic passage switch, the electrophoretic passage switch is located between electrophoretic passage anode end and glue injection pump's pipeline, is used for opening or closing electrophoretic passage anode end and the electrophoretic passage between cathode end, electrophoretic passage anode end and cathode end are connected with glue injection pump through the pipeline respectively, and electrophoretic passage anode end is equipped with exhaust hole, and the exhaust hole is connected with gas collection device, and electrophoretic passage cathode end connects capillary, glue injection pump absorbs glue and closes electrophoretic passage switch to make the pressure in passage, opens the switch after the pressure reaches the standard, and the bubble in glue injection pump is discharged through anode end exhaust hole, and then glue is injected to capillary to discharge the bubble in capillary. The utility model discloses through structural design realizes the efficient discharge of electrophoretic passage and capillary bubble, saves gel, improves separation efficiency, and protects instrument and capillary.

Description

Technical Field

[0001] This utility model relates to the field of capillary gel electrophoresis technology, specifically to a device for removing air bubbles in an electrophoresis pathway. Background Technology

[0002] Capillary gel electrophoresis (CGE) uses a high-pressure gel pump to fill a capillary with a gel or gel-like medium, achieving efficient separation based on differences in molecular size through the molecular sieve effect. However, air bubbles are easily drawn in during the gel pump's suction and dispensing process. If these air bubbles enter the capillary or electrophoresis path, they can lead to abnormal current and electric field, decreased separation efficiency, abnormal detection signals, and damage to the instrument and capillary.

[0003] In existing technologies, air bubbles are usually removed by repeatedly injecting a large amount of gel to carry out the bubbles, or by centrifuging the gel to remove dissolved gases. However, this results in gel waste and poor economic efficiency, and air bubbles entering the pipeline during gel replacement cannot be avoided. Utility Model Content

[0004] The purpose of this invention is to provide a device for removing air bubbles in an electrophoresis pathway, which solves the problems of gel waste and incomplete removal of air bubbles in the pipeline during gel replacement in the prior art.

[0005] To achieve the above objectives, this utility model provides the following technical solution: An electrophoresis channel bubble removal device includes an electrophoresis channel anode (1), an electrophoresis channel cathode (5), a glue injection pump (3), a glue suction port (4), a capillary tube (6), and an electrophoresis channel switch (2). The positive end (1) and negative end (5) of the electrophoresis channel are respectively connected to the glue injection pump (3) through pipelines, and the glue injection pump (3) is provided with a glue suction port (4). The electrophoresis path switch (2) is located on the pipeline between the electrophoresis path anode (1) and the glue injection pump (3) and is used to open or close the electrophoresis path between the electrophoresis path anode (1) and the electrophoresis path cathode (5). A capillary tube (6) is connected between the negative end (5) of the electrophoresis channel and the glue injection pump (3). The glue injection pump (3) can inject glue into the capillary tube (6) to fill the capillary tube and expel air bubbles inside.

[0006] Preferably, the electrophoresis circuit switch (2) is an electromagnetic valve or a manual valve, which facilitates the control of the opening and closing of the circuit.

[0007] Preferably, a pressure sensor is provided on the electrophoresis channel to monitor the pressure in the channel in real time and ensure that the electrophoresis channel switch is turned on to discharge the air bubbles in the glue injection pump (3) when the pressure reaches a preset threshold.

[0008] Preferably, the anode end (1) of the electrophoresis passage is provided with an exhaust hole (7) for discharging air bubbles.

[0009] Preferably, the vent (7) is located at the tail end of the anode end (1) of the electrophoresis channel, and the vent (7) is connected to a gas collection device (8) to facilitate the collection of discharged bubbles and avoid contamination; the vent (7) has a diameter of 0.5-1.5 mm, which can reduce gel loss.

[0010] Preferably, the connection between the positive end (1) of the electrophoresis channel and the glue injection pump (3) is in a horizontal position higher than the connection between the negative end (5) of the electrophoresis channel and the glue injection pump (3).

[0011] The beneficial effects of this utility model are as follows: (1) No need for multiple injections; precise defoaming through structural design reduces gel waste. (2) It can effectively remove air bubbles that enter the pipeline during the gel replacement process, and avoid air bubble residue in the electrophoresis path and capillary. (3) Improves separation efficiency, protects instruments and capillaries, and is highly practical. Attached Figure Description

[0012] To more clearly illustrate the technical solutions of this utility model, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0013] Figure 1 This is a structural diagram of an electrophoresis pathway bubble removal device.

[0014] Explanation of reference numerals in the attached diagram: 1. Electrophoresis path positive terminal; 2. Electrophoresis path switch; 3. Glue injection pump; 4. Glue suction port; 5. Electrophoresis path negative terminal; 6. Capillary tube; 7. Exhaust port; 8. Gas collection device. Detailed Implementation

[0015] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0016] This utility model is a device for removing air bubbles in an electrophoresis channel, such as... Figure 1As shown, an electrophoresis circuit switch (2) is set at the positive end (1) of the electrophoresis circuit. The electrophoresis circuit switch (2) is used to open or close the electrophoresis circuit between the positive end (1) and the negative end (5) of the electrophoresis circuit. The positive end (1) and the negative end (5) of the electrophoresis circuit are respectively connected to the glue injection pump (3) through pipelines.

[0017] The electrophoresis path switch (2) is an electromagnetic valve or a manual valve, which is installed on the pipeline between the positive end (1) of the electrophoresis path and the glue injection pump (3) to realize the automatic or manual opening and closing of the electrophoresis path between the positive end (1) and the negative end (5) of the electrophoresis path.

[0018] During the process of the glue pump (3) drawing glue from the glue inlet (4), the air bubbles present in the glue pump will be suspended in the upper part of the glue pump (3), and the electrophoresis circuit switch (2) will be closed, so that pressure is generated in the electrophoresis circuit.

[0019] A pressure sensor is installed in the electrophoresis path to monitor the pressure inside the electrophoresis path in real time.

[0020] When the pressure in the electrophoresis channel reaches the preset pressure threshold, the electrophoresis channel switch (2) is turned on, and the air bubbles in the injection pump (3) are discharged from the positive end (1) of the electrophoresis channel.

[0021] The range of the preset pressure threshold is 70% to 90% of the maximum working pressure of the dispensing pump (3), in megapascals.

[0022] The above-mentioned opening of the electrophoresis pathway switch (2) and the expulsion of air bubbles from the anode end (1) of the electrophoresis pathway by the gel pump (3) specifically includes the following steps: When the electrophoresis path switch (2) is turned on, due to the excessive pressure in the capillary tube (6) between the cathode end (5) of the electrophoresis path and the dispensing pump (3), a pressure release will occur in the electrophoresis path from the dispensing pump (3) to the anode end (1) of the electrophoresis path at the moment of opening. Then, a gushing phenomenon will be generated at the anode end (1) of the electrophoresis path through this pressure release. The duration of the pressure release is 500 milliseconds to 2000 milliseconds, and the flow rate generated by the gushing phenomenon is 3.5 times to 5 times the rated flow rate of the dispensing pump. The air bubbles in the gel pump (3) are discharged from the anode end (1) of the electrophoresis channel through the gushing phenomenon of the anode end (1) of the electrophoresis channel.

[0023] Because the inner diameter of the tube at the positive end (1) of the electrophoresis pathway is small, the amount of gel medium or gel-like medium consumed each time air bubbles are expelled is also very small.

[0024] The anode end (1) of the above electrophoresis channel is provided with an exhaust hole (7), and the air bubbles are discharged through the exhaust hole (7). The diameter of the exhaust hole is 0.5-1.5 mm. The above-mentioned vent (7) is located at the tail end of the anode end (1) of the electrophoresis passage, and the vent (7) is connected to a gas collection device (8).

[0025] After the air bubbles are expelled from the injection pump (3), the glue is injected into the capillary (6) between the negative end (5) of the electrophoresis channel and the injection pump (3) to fill the capillary and expel the air bubbles in the capillary.

[0026] The above-mentioned suction and injection refer to the suction and injection of gel medium or gel-like medium. The gel medium or gel-like medium is centrifuged to remove dissolved gases. The centrifugation conditions are 8000 to 12000 rpm, and the centrifugation time is 4 to 6 minutes.

[0027] In order to achieve better results, the connection between the positive end (1) of the electrophoresis channel and the gel pump (3) can be positioned horizontally higher than the connection between the negative end (5) of the electrophoresis channel and the gel pump (3).

[0028] In summary, the electrophoresis pathway bubble removal device provided in this application has the following technical effects: (1) No need for multiple injections; precise defoaming through structural design reduces gel waste. (2) It can effectively remove air bubbles that enter the pipeline during the gel replacement process, and avoid air bubble residue in the electrophoresis path and capillary. (3) Improves separation efficiency, protects instruments and capillaries, and is highly practical.

[0029] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0030] The above description of the disclosed embodiments enables those skilled in the art to make or use this application. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of this application. Therefore, this application is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A device for removing air bubbles in an electrophoresis pathway, characterized in that, include: Electrophoresis pathway anode (1), electrophoresis pathway cathode (5), dispensing pump (3), dispensing port (4), capillary tube (6) and electrophoresis pathway switch (2); The positive end (1) and negative end (5) of the electrophoresis channel are respectively connected to the glue injection pump (3) through pipelines, and the glue injection pump (3) is provided with a glue suction port (4). The electrophoresis path switch (2) is located on the pipeline between the electrophoresis path anode (1) and the glue injection pump (3) and is used to open or close the electrophoresis path between the electrophoresis path anode (1) and the electrophoresis path cathode (5). A capillary tube (6) is connected between the negative end (5) of the electrophoresis channel and the glue injection pump (3). The glue injection pump (3) can inject glue into the capillary tube (6) to fill the capillary tube and expel air bubbles inside.

2. The electrophoresis channel bubble removal device as described in claim 1, characterized in that, The electrophoresis circuit switch (2) is an electromagnetic valve or a manual valve.

3. The electrophoresis pathway bubble removal device as described in claim 1, characterized in that, A pressure sensor is installed on the electrophoresis path to monitor the pressure inside the electrophoresis path.

4. The electrophoresis pathway bubble removal device as described in claim 1, characterized in that, The anode end (1) of the electrophoresis channel is provided with an exhaust port (7) for discharging air bubbles.

5. The electrophoresis pathway bubble removal device as described in claim 4, characterized in that, The vent (7) is located at the tail end of the anode end (1) of the electrophoresis passage, and the vent (7) is connected to a gas collection device (8).

6. The electrophoresis pathway bubble removal device as described in claim 1, characterized in that, The connection between the positive end (1) of the electrophoresis pathway and the glue injection pump (3) is horizontally higher than the connection between the negative end (5) of the electrophoresis pathway and the glue injection pump (3).

Images