A colloidal gold kit based on a sealed film integrated anti-pollution design

Abstract

The utility model relates to in-vitro diagnosis technical field discloses a colloidal gold reagent box based on sealed film integration anti -pollution design, including casing, first sealed film, second sealed film and detection component, wherein, detection component sets up in the casing inside, and the casing outside is provided with the groove body, is equipped with the sample adding hole, observation hole and water absorbing hole on the groove body, and the sample adding hole, observation hole and water absorbing hole all are linked together with the casing inside, and the sample adding hole is used for adding sample to detection component, and the observation hole is provided with transparent observation window, and the observation hole corresponds to the result display area of detection component, and the water absorbing hole is full of water absorbing paper, and the first sealed film and second sealed film cooperate and attach to the surface of groove body, and the first sealed film covers sample adding hole at least, and the second sealed film covers observation hole and water absorbing hole simultaneously at least. The utility model discloses through integration sealed film design and casing optimization, solves the pollution risk and the operating complexity problem of existing packaging.

Description

Technical Field

[0001] This utility model relates to the field of in vitro diagnostic technology, and in particular to a colloidal gold reagent kit based on an integrated sealing membrane anti-contamination design. Background Technology

[0002] Colloidal gold chromatography, due to its speed and convenience, has become an important tool in the field of in vitro diagnostics. However, the packaging design of existing colloidal gold reagent kits has significant flaws. The sample dispensing port and result display area are constantly exposed to the external environment, making them susceptible to contaminants, leading to sample contamination or test strip failure. Traditional packaging typically requires users to disassemble individual components multiple times, such as blood collection tubes and droppers, making the process cumbersome and increasing the complexity of home use. Furthermore, in existing technologies, the test strip is directly exposed to the air after sample dispensing, posing a risk of cross-contamination, especially in scenarios involving bodily fluid samples such as blood and saliva.

[0003] While existing technologies have attempted to reduce the risk of contamination by encapsulating the sample dispensing port with aluminum-plastic film, their designs are not optimized for the chromatographic characteristics of colloidal gold test strips. For example, the result display area lacks independent sealing protection, and the interpretation window may still be affected by environmental interference; the outer casing structure is complex and lacks a portable design suitable for home use. Utility Model Content

[0004] In view of the shortcomings of the prior art described above, the purpose of this utility model is to provide a colloidal gold reagent kit based on an integrated sealing membrane anti-contamination design. Through the integrated sealing membrane design and shell optimization, the contamination risk and operational complexity of existing packaging are solved, and the entire process of sample addition, chromatography, and interpretation is closed, which significantly improves the home applicability of the colloidal gold reagent kit.

[0005] The present invention adopts the following technical solution:

[0006] A colloidal gold reagent kit based on an integrated sealing membrane anti-contamination design includes a shell, a first sealing membrane, a second sealing membrane, and a detection component. The detection component is disposed inside the shell, and a groove is provided on the outer side of the shell. The groove has a sample application port, an observation port, and a water absorption port, all of which communicate with the interior of the shell. The sample application port is used to add a sample to the detection component. The observation port has a transparent observation window corresponding to the result display area of ​​the detection component. The water absorption port is filled with absorbent paper. The first and second sealing membranes are attached to the surface of the groove, with the first sealing membrane at least covering the sample application port and the second sealing membrane at least simultaneously covering both the observation port and the water absorption port.

[0007] Preferably, in the above-mentioned colloidal gold reagent kit based on the integrated anti-contamination design of the sealing membrane, both the first sealing membrane and the second sealing membrane are J-type laminated membranes.

[0008] Preferably, the colloidal gold reagent kit based on the integrated anti-contamination design of the sealing membrane further includes a handheld end and a handle; wherein the handheld end is disposed on one side of the housing, and the handle is installed on the outside of the housing.

[0009] Preferably, in the above-mentioned colloidal gold reagent kit based on the integrated anti-pollution design of the sealing film, the outer surface of the handheld end is provided with anti-slip protrusions.

[0010] Preferably, in the above-mentioned colloidal gold reagent kit based on the integrated anti-fouling design of the sealing membrane, the interior of the first sealing membrane is provided with a first hydrophobic coating.

[0011] Preferably, in the above-mentioned colloidal gold reagent kit based on the integrated anti-fouling design of the sealing membrane, the interior of the second sealing membrane is provided with a second hydrophobic coating.

[0012] Preferably, in the above-mentioned colloidal gold reagent kit based on the integrated anti-contamination design of the sealing membrane, the detection component includes a PVC plate, a sample pad, a marker-binding pad, nitrocellulose paper, a detection line, a control line, and an absorbent layer; wherein the sample pad, the marker-binding pad, the nitrocellulose paper, and the absorbent layer are all disposed on the PVC plate, a portion of the marker-binding pad is disposed at the lower end of the sample pad, a portion of the marker-binding pad is disposed at the upper end of the nitrocellulose paper, the detection line and the control line are disposed on the nitrocellulose paper, and a portion of the absorbent layer is disposed at the upper end of the nitrocellulose paper.

[0013] Preferably, in the above-mentioned colloidal gold reagent kit based on the integrated anti-contamination design of the sealing membrane, a first flow-guiding gap is provided between the marker binding pad and the nitrocellulose paper.

[0014] Preferably, in the above-mentioned colloidal gold reagent kit based on the integrated anti-fouling design of the sealing membrane, a second flow guiding gap is provided between the absorbent layer and the nitrocellulose paper.

[0015] Preferably, in the above-mentioned colloidal gold reagent kit based on the integrated anti-pollution design of the sealing membrane, the width of the first flow channel gap is 1.5 to 2.5 mm.

[0016] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0017] The independent sealing design of the sample dispensing port and result display area achieves zero contact throughout the operation, significantly reducing the risk of cross-infection. The integrated shell structure combined with the non-slip handle simplifies the operation steps, allowing for home self-testing without professional training. The application of hot-pressing technology and standardized components reduces production costs, while the flow-guiding gap design ensures uniform and efficient chromatography, delivering reliable results within 15 minutes. This invention is the first to combine a J-type overlapping sealing film with colloidal gold chromatography technology, providing a safe, convenient, and low-cost packaging solution for in vitro diagnostics, with broad market application prospects. Attached Figure Description

[0018] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on the structures shown in these drawings without creative effort.

[0019] Figure 1 This is a structural diagram of a colloidal gold reagent kit based on an integrated anti-contamination design with a sealing film, according to an embodiment of the present invention, in the state where the sealing film is not torn open.

[0020] Figure 2 This is a structural diagram of a colloidal gold reagent kit based on an integrated anti-contamination design with a sealing film, according to an embodiment of the present invention, with the sealing film torn off.

[0021] Figure 3 This is a structural diagram of the detection component of a colloidal gold reagent kit based on an integrated anti-contamination design with a sealing membrane, according to an embodiment of the present invention.

[0022] Figure 4 This is a structural diagram of a colloidal gold reagent kit based on an integrated sealing membrane anti-pollution design according to an embodiment of the present invention, when anti-slip protrusions are provided;

[0023] Figure 5 This is a cross-sectional view of the first and second sealing films of a colloidal gold reagent kit based on an integrated anti-contamination design according to an embodiment of the present invention.

[0024] Explanation of reference numerals in the attached figures:

[0025] 1. Shell; 2. First sealing membrane; 21. First hydrophobic coating; 3. Second sealing membrane; 31. Second hydrophobic coating; 4. Detection component; 41. PVC board; 42. Sample pad; 421. First sample pad body; 422. Second sample pad body; 43. Marker binding pad; 44. Nitrocellulose paper; 45. Detection line; 46. Quality control line; 47. Absorbent layer; 48. First flow guide gap; 49. Second flow guide gap; 5. Tank; 6. Sample dispensing hole; 7. Observation hole; 8. Absorption hole; 9. Transparent observation window; 10. Absorbent paper; 11. Handheld end; 12. Handle; 13. Anti-slip protrusions. Detailed Implementation

[0026] The following specific examples illustrate the implementation of this utility model. Those skilled in the art can easily understand other advantages and effects of this utility model from the content disclosed in this specification. This utility model can also be implemented or applied through other different specific embodiments, and various details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of this utility model. It should be noted that, unless otherwise specified, the following embodiments and features described therein can be combined with each other.

[0027] In the description of this utility model, unless otherwise stated, "a plurality of" means two or more; the terms "upper," "lower," "left," "right," "inner," "outer," "front end," "rear end," "head," "tail," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. In addition, the terms "first," "second," "third," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0028] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "connected" and "linked" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0029] The present invention will now be further described with reference to the accompanying drawings.

[0030] This utility model embodiment provides a colloidal gold reagent kit based on an integrated sealing membrane anti-contamination design, such as... Figures 1 to 3As shown, the colloidal gold reagent kit based on the integrated anti-contamination design of the sealing membrane includes a shell 1, a first sealing membrane 2, a second sealing membrane 3, and a detection component 4. The detection component 4 is disposed inside the shell 1, and a groove 5 is disposed on the outside of the shell 1. The groove 5 has a sample application hole 6, an observation hole 7, and a water absorption hole 8. The sample application hole 6, the observation hole 7, and the water absorption hole 8 are all connected to the inside of the shell 1. The sample application hole 6 is used to add samples to the detection component 4. The observation hole 7 is provided with a transparent observation window 9, which corresponds to the result display area of ​​the detection component 4. The water absorption hole 8 is filled with absorbent paper 10. The first sealing membrane 2 and the second sealing membrane 3 are attached to the surface of the groove 5. The first sealing membrane 2 covers at least the sample application hole 6, and the second sealing membrane 3 covers at least both the observation hole 7 and the water absorption hole 8.

[0031] The first sealing membrane 2 covers the sample application port 6. During storage and transportation, the first sealing membrane 2 completely seals the sample application port 6, preventing environmental pollutants (such as dust, moisture, microorganisms, etc.) from entering the housing 1 and protecting the detection component 4. In use, the user only needs to remove the first sealing membrane 2 to expose the sample application port 6. The second sealing membrane 3 simultaneously covers the observation port 7 and the absorbent hole 8. Before and during sample application, the second sealing membrane 3 always simultaneously seals both the observation port 7 of the transparent observation window 9 and the absorbent hole 8 containing the absorbent paper 10. The second sealing membrane 3 remains sealed during the sample application step. The user only needs to remove the second sealing membrane 3 after sample application is complete.

[0032] Therefore, when using this colloidal gold reagent kit, during the sample loading stage, the user removes the first sealing membrane 2, which only covers the sample loading well 6. The sample is then added to the loading area (typically the sample pad) of the detection component 4 through the exposed sample loading well 6. At this time, the observation well 7 and the absorbent well 8 are still tightly covered by the second sealing membrane 3, preventing sample splashing / aerosol contamination of the transparent observation window 9. Any splashing or aerosol that may occur during sample loading is blocked by the second sealing membrane 3 and cannot fall onto the transparent observation window 9 inside the observation well 7. The second sealing membrane 3 covers the absorbent well 8, preventing the user from accidentally coming into contact with the absorbent paper 10 and any potentially pathogen-containing waste liquid it absorbs during sample loading. Keeping the absorbent well 8 sealed before sample loading prevents the absorbent paper 10 from becoming damp and affecting its absorbency.

[0033] After sample addition, the user removes the second sealing membrane 3, exposing the observation well 7 and the absorption well 8. The target analyte in the sample binds to the captured substance on the detection line (T line) of the detection component 4, causing colloidal gold aggregation and color development. Simultaneously, color development should also occur at the control line (C line). The user clearly observes the result display area (T line and C line) on the detection component 4 corresponding to the observation well 7 through the transparent observation window 9 on the observation well 7, which is uncontaminated by the sample addition process, and interprets the test result (positive / negative / invalid).

[0034] This colloidal gold reagent kit utilizes a first sealing membrane 2 (dedicated to the sample dispensing well 6) and a second sealing membrane 3 (simultaneously covering the observation well 7 and the absorbent well 8) to achieve time-sequential membrane removal and zoned protection. The key function of the second sealing membrane 3 is to completely physically isolate the transparent observation window 9 within the observation well 7 and the absorbent paper 10 within the absorbent well 8 from potential contaminants generated during the sample dispensing process and from user operations during the sample dispensing step. This effectively solves the problems of contamination of the observation window and user contact with waste liquid in traditional reagent kits, significantly improving the accuracy, reliability, operational safety, and user experience of the colloidal gold reagent kit.

[0035] In some embodiments, both the first sealing membrane 2 and the second sealing membrane 3 are J-type laminated membranes. The use of J-type laminated membranes simplifies and standardizes the peeling process, eliminating the need for users to locate specific peeling points or worry about tearing, resulting in smoother operation, better ergonomics, and faster detection. As an example only, the J-type laminated membrane is formed using a pre-indentation process, breaking in one go along the pre-cut path during tearing, ensuring that the operating area is exposed only once, avoiding secondary contamination. The inner layer of the sealing membrane is coated with a hydrophobic coating to prevent liquid penetration into the membrane gaps, further reducing the risk of cross-contamination.

[0036] In some embodiments, such as Figure 1 and Figure 2 As shown, the colloidal gold reagent kit based on the integrated anti-contamination design of the sealing membrane also includes a handheld end 11 and a handle 12; wherein, the handheld end 11 is disposed on one side of the housing 1, and the handle 12 is installed on the outside of the housing 1.

[0037] Most current colloidal gold reagent kits are designed for gripping, which can lead to samples falling onto the hand during dispensing. Therefore, this embodiment adds a handheld end 11 and a handle 12. When using this colloidal gold reagent kit, the user can hold it using the handheld end 11 and handle 12, keeping the hand as far away from the dispensing area and result display area as possible. This provides convenience and prevents samples from falling onto the user's hand. Furthermore, a stable grip helps with accurate dispensing and clear observation, further ensuring accurate result interpretation.

[0038] In some embodiments, such as Figure 4 As shown, the outer surface of the handheld end 12 is provided with anti-slip protrusions 13.

[0039] For example, the handheld end 12 can be injection molded from TPU material and have anti-slip bumps distributed on its surface to provide stable friction when gripped.

[0040] In some embodiments, such as Figure 5 As shown, Figure 5(a) and (b) are cross-sectional views of the first sealing membrane 2 and the second sealing membrane 3, respectively. The first sealing membrane 2 has a first hydrophobic coating 21 inside, and the second sealing membrane 3 has a second hydrophobic coating 31 inside. The first hydrophobic coating 21 and the second hydrophobic coating 31 have the same function, both of which are used to prevent liquid from penetrating into the gaps between the membrane layers, thereby further reducing the risk of cross-infection.

[0041] In some embodiments, such as Figure 3 As shown, the detection component 4 includes a PVC plate 41, a sample pad 42, a marker binding pad 43, nitrocellulose paper 44, a detection line 45, a control line 46, and an absorbent layer 47. The sample pad 42, the marker binding pad 43, the nitrocellulose paper 44, and the absorbent layer 47 are all disposed on the PVC plate 41. A portion of the marker binding pad 43 is disposed at the lower end of the sample pad 42, and a portion of the marker binding pad 43 is disposed at the upper end of the nitrocellulose paper 44. The detection line 45 and the control line 46 are disposed on the nitrocellulose paper 44, and a portion of the absorbent layer 47 is disposed at the upper end of the nitrocellulose paper 44.

[0042] After the corresponding sample is added, the sample dissolves or diffuses on the sample pad 42. The sample solution flows towards the label conjugation pad 43 via capillary action. The label conjugation pad 43 is pre-embedded with specific biomolecules (such as antibodies or antigens) labeled with colloidal gold. The target analyte in the sample binds to the colloidal gold label on the label conjugation pad 43, forming a complex. The complex and liquid continue to flow forward to the nitrocellulose paper 44. The nitrocellulose paper 44 has a pre-set detection line 45 (embedded with a substance that captures the target analyte, such as an antibody / antigen) and a control line 46 (embedded with a universal capture substance, such as an antigold-labeled antibody / secondary antibody). When the sample solution flows through the detection line 45, if the sample contains the target analyte, the target analyte-colloidal gold label complex will be captured and aggregated by the capture substance on the detection line 45, forming a visible detection line 45 (T line). Regardless of whether the sample contains the target analyte, excess colloidal gold label or complex will be captured and aggregated by the capture substance on the control line 46, forming a visible control line 46 (C line). The color development at control line 46 indicates that the chromatography process has been completed normally. The liquid continues to flow forward to absorbent layer 47. Absorbent layer 47 continuously absorbs the liquid flowing through nitrocellulose paper 44 through capillary action, providing a continuous driving force for chromatography. Absorbent layer 47 is finally connected to absorbent paper 10 within absorbent holes 8, which is the main waste liquid absorption and buffering area.

[0043] After adding the sample, wait for the set reaction time. The user removes the second sealing film 3, exposing the observation well 7 and the absorbent hole 8. The user holds the handle 11 of the housing 1 or grasps the handle 12 to keep the reagent kit stable and aligns the observation well 7 with their line of sight. Through the transparent observation window 9, directly observe the color development of the result display area on the nitrocellulose paper 44 corresponding to the area of ​​the observation well 7, namely the test line 45 and the control line 46. Based on whether the test line 45 (T line) and the control line 46 (C line) develop color and the intensity of the color development, interpret the test result (positive, negative, invalid).

[0044] The second sealing membrane 3 covers the observation hole 7 during sample application, preventing sample splashes from contaminating the transparent observation window 9 and the areas of the test line 45 and control line 46 on the nitrocellulose paper 44 directly below it, thus completely eliminating false positives, false negatives, or misjudgments caused by this. The sealed design protects the marker binding pad 43 and nitrocellulose paper 44 from environmental influences during storage and transportation. The J-shaped overlapping membrane is removed cleanly, with no residual adhesive / fragment obstructing the transparent observation window 9; the handheld end 11 / handle 12 ensures a stable grip, allowing the observation hole 7 to be directly in the user's line of sight for clear observation of the test line 45 and control line 46.

[0045] In some embodiments, such as Figure 3 As shown, a first flow-guiding gap 48 is provided between the marker bonding pad 43 and the nitrocellulose paper 44, and a second flow-guiding gap 49 is provided between the absorbent layer 47 and the nitrocellulose paper 44.

[0046] In some embodiments, the width of the first flow guide gap 48 is 1.5 to 2.5 mm. This first flow guide gap 48 is used to optimize the chromatography flow rate.

[0047] In some embodiments, such as Figure 3 As shown, the sample pad 42 includes a first sample pad body 421 and a second sample pad body 422, which are joined together by a hot-pressing process. The first sample pad body 421 is fixed to the front end of the PVC board 41, and the second sample pad body 422 is connected to the nitrocellulose paper 44 through a marker bonding pad 43. The sample pad 42 adopts a double-layer glass fiber structure, with a coarse fiber layer (pore size 20-40 μm) on top and a fine fiber layer (pore size 5-10 μm) on the bottom, which is used to absorb samples and filter impurities, thereby improving detection accuracy.

[0048] In some embodiments, such as Figure 3 As shown, the labeled conjugate pad 43 is disposed at the rear end of the sample pad 42 and is coated with colloidal gold labeled particles with a diameter of 40 nm. The colloidal gold particles are prepared by sodium citrate reduction and their surface is modified with target-specific antibodies. The labeled conjugate pad is uniformly sprayed using a gold spray gun at a flow rate of 0.1 μL / mm, and after drying, a stable binding layer is formed, ensuring that the labeled particles bind efficiently to the target analyte during chromatography.

[0049] In some embodiments, nitrocellulose paper 44 is fixed to the middle of PVC board 12, with its front end bonded to marker binding pad 43 by UV adhesive and its rear end connected to absorbent layer 47. Target-specific antibodies are sprayed onto the nitrocellulose paper 44 to form detection lines 45 and control lines 46. The spraying process employs non-contact micro-spotting technology, with the line width controlled within 0.5 mm to ensure clear and easily readable color development.

[0050] In some embodiments, the absorbent layer 47 is selected as a highly absorbent resin layer, which is bonded to the end of the nitrocellulose paper 4 with UV adhesive, and a second flow guide gap 49 of 1.5 to 2.5 mm can be reserved between the two. The second flow guide gap 49 is formed by precision molding, and the chromatography rate is controlled by capillary action to make the sample flow through the detection line and the control line at a uniform speed, so as to avoid false positives or false negatives caused by uneven flow rate.

[0051] In some embodiments, an application example of this colloidal gold reagent kit based on an integrated anti-contamination design with a sealed membrane is provided. In this application example, based on the significant positive correlation between Golgi protein 73 (GP73) in blood and HPVG, a method for predicting portal hypertension is developed, using GP73 as a specific antigen, which is then combined with specific colloidal gold antibody particles on the detection kit. Figures 1 to 3 As shown, after the user removes the first sealing film 2 covering the sample application well 6, the collected fingertip blood sample is dropped onto the sample pad 42. The sample migrates sequentially to the marker binding pad 43 via chromatography and binds to the GP73 colloidal gold marker to form a complex. The complex continues to migrate along the nitrocellulose membrane 44. The detection line (T line) 45 captures the target analyte and develops color, while the control line (C line) 46 verifies the detection validity. After 15 minutes, the results are observed through the result display area. The reaction results are displayed through the detection line (T line) 45 and the control line (C line) 46 on the nitrocellulose membrane 44, typically exhibiting four scenarios:

[0052] First scenario: Both test line 45 and control line 46 are visible, indicating a positive test result.

[0053] Second scenario: Only the control line 46 is displayed, indicating that the test result is negative.

[0054] Third scenario: Only the detection line 45 is displayed, indicating that the detection result is invalid.

[0055] Fourth scenario: If neither test line 45 nor control line 46 is displayed, the test result is invalid.

[0056] It should be noted that a positive result indicates that the current test result shows that the portal vein pressure exceeds the set threshold, indicating a risk of portal hypertension. A negative result indicates that the portal vein pressure does not exceed the set threshold and is considered normal. An invalid result indicates that the current test has failed. The reasons for failure may include improper operation, use of non-target samples, or problems with components in the kit, such as GP73 colloidal gold labeling falling off or the GP73 colloidal gold labeling exceeding its expiration date.

[0057] This minimally invasive home portal vein pressure monitoring kit has advantages such as high specificity, high timeliness, and ease of operation, allowing patients to monitor their condition at home.

[0058] With high specificity, after the patient obtains the test results from the kit, the real-time portal pressure prediction value for cirrhosis and upper gastrointestinal bleeding can be used to assess the risk of serious complications such as gastrointestinal bleeding, achieving early warning and ensuring the patient's life safety to the greatest extent and with the highest efficiency. Its specificity is as high as 96% or more.

[0059] For high timeliness, the minimally invasive portal vein pressure home testing kit proposed in this embodiment provides results in just 15 minutes, offering rapid and efficient testing. This kit requires only a small amount of blood from a finger prick, making it easy to use and suitable not only for medical institutions but also for home self-testing.

[0060] Regarding ease of operation, this utility model offers advantages over other product testing methods: Currently, the only marketable method for predicting hepatic venous pressure gradient (HVPG) measurement in cirrhosis and meeting the decompensated standard is an invasive procedure with high technical requirements and high cost, limited to only a few centers, thus restricting its clinical application. The reagent kit proposed in this utility model is not only low-cost but also overcomes the limitations of invasiveness, achieving minimally invasive testing. Furthermore, it is convenient and easy to operate, breaking the current limitation of testing methods being confined to large hospitals and making it accessible to the general public.

[0061] The above embodiments are only used to illustrate the technical solutions of this utility model, and are not intended to limit it. Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of this utility model, and they should all be covered within the scope of the claims and specification of this utility model.

Claims

1. A colloidal gold kit based on a sealed membrane integrated anti-pollution design, characterized in that, The device includes a housing, a first sealing membrane, a second sealing membrane, and a detection component. The detection component is disposed inside the housing, and a groove is provided on the outer side of the housing. The groove has a sample application hole, an observation hole, and a water absorption hole, all of which communicate with the interior of the housing. The sample application hole is used to add a sample to the detection component. The observation hole has a transparent observation window corresponding to the result display area of ​​the detection component. The water absorption hole is filled with absorbent paper. The first and second sealing membranes are attached to the surface of the groove, with the first sealing membrane at least covering the sample application hole and the second sealing membrane at least simultaneously covering both the observation hole and the water absorption hole.

2. The colloidal gold kit based on the integration of the sealed membrane and the anti-pollution design according to claim 1, characterized in that, Both the first sealing film and the second sealing film are J-type laminated films.

3. The colloidal gold reagent kit based on the integrated sealing membrane anti-contamination design according to claim 1, characterized in that, It also includes a handheld end and a handle; wherein the handheld end is disposed on one side of the housing, and the handle is mounted on the outside of the housing.

4. The colloidal gold kit based on the integrated anti-pollution design of the sealing film according to claim 3, characterized in that, The outer surface of the handheld end is provided with anti-slip protrusions.

5. The colloidal gold kit based on the integration of the sealed membrane and the anti-pollution design according to claim 1, characterized in that, The first sealing membrane has a first hydrophobic coating inside.

6. The colloidal gold kit based on the integration of the sealed membrane and the anti-pollution design according to claim 1, characterized in that, The interior of the second sealing membrane is provided with a second hydrophobic coating.

7. The colloidal gold kit based on the integration of a sealing film and an anti-pollution design according to any one of claims 1 to 6, characterized in that, The detection assembly includes a PVC board, a sample pad, a marker-binding pad, nitrocellulose paper, a detection line, a control line, and an absorbent layer. The sample pad, marker-binding pad, nitrocellulose paper, and absorbent layer are all disposed on the PVC board. A portion of the marker-binding pad is disposed at the lower end of the sample pad, and a portion of the marker-binding pad is disposed at the upper end of the nitrocellulose paper. The detection line and the control line are disposed on the nitrocellulose paper, and a portion of the absorbent layer is disposed at the upper end of the nitrocellulose paper.

8. The colloidal gold kit based on the integration of the sealed membrane and the anti-pollution design according to claim 7, characterized in that, A first flow-guiding gap is provided between the marker binding pad and the nitrocellulose paper.

9. The colloidal gold kit based on the integration of the sealed membrane and the anti-pollution design according to claim 7, characterized in that, A second flow-guiding gap is provided between the absorbent layer and the nitrocellulose paper.

10. The colloidal gold reagent kit based on the integrated sealing membrane anti-contamination design according to claim 7, characterized in that, The width of the first flow guide gap is 1.5 to 2.5 mm.

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