An experimental device for assisting the intervention of cell growth by ultrasound

Abstract

The utility model relates to the experimental apparatus of auxiliary ultrasonic action intervention cell growth, experimental apparatus includes electric heating box, first mobile subassembly, second mobile subassembly and ultrasonic wave subassembly, the hole plate and liquid are placed in electric heating box, electric heating box is used for heating liquid to set temperature to maintain set temperature unchangeable, and liquid is used to carry out water bath heating to multiple petri dishes on the hole plate, first mobile subassembly is connected in the box bottom, is suitable for along the length direction of electric heating box movement, second mobile subassembly is connected with first mobile subassembly, is suitable for along the width direction of electric heating box movement, transducer is connected on second mobile subassembly, and the top is connected with joint subassembly, and first mobile subassembly and second mobile subassembly work can drive transducer and joint subassembly to move between multiple petri dishes on the hole plate. Joint subassembly points to the hole plate, is used for focusing the ultrasonic wave that transducer emits on the corresponding petri dish, to know the treatment mechanism of ultrasonic technology, to facilitate clinical application.

Description

Technical Field

[0001] This utility model relates to the field of medical device technology, and in particular to an experimental device for assisting ultrasound in intervening in cell growth. Background Technology

[0002] Ultrasound, as a type of mechanical wave, is not only used for medical diagnosis, but also, due to its non-invasive nature, good penetration, and directivity, can stimulate target tissues, promote cell repair, regulate inflammatory responses, and improve tissue function. Currently, it has demonstrated its potential therapeutic value in multiple medical fields, including the treatment of neurological diseases, musculoskeletal system repair, anti-inflammatory and immunotherapeutic applications, and tumor treatment.

[0003] Before this technology can be applied clinically, its therapeutic mechanism needs to be systematically studied and validated at the cellular level to ensure its safety, efficacy, reproducibility, and standardization.

[0004] Currently, experiments involving ultrasound intervention in cell growth are generally conducted at room temperature. However, since room temperature is not constant, cells may deviate from the optimal culture temperature (37°C), leading to changes in their biological behavior and phenotype, which affects the experimental results. Secondly, different transducers are required for the experiments, and the far-field and focal positions of the acoustic waves vary for each transducer. This necessitates repeated adjustments to the transducer and well plate positions during the experiment, which is inconvenient.

[0005] Therefore, there is an urgent need for an experimental device that can provide a constant temperature environment and adjust the far field and focal position of ultrasound for different transducers, so as to objectively and accurately understand the treatment mechanism of ultrasound technology for clinical application. Utility Model Content

[0006] The present invention aims to solve at least one of the technical problems existing in the prior art or related technologies.

[0007] Therefore, this invention provides an experimental device for assisting ultrasound intervention in cell growth, which can provide a constant temperature environment and adjust the far field and focal position of ultrasound waves for different transducers, so as to objectively and accurately understand the therapeutic mechanism of ultrasound technology for clinical application.

[0008] An experimental apparatus for assisting ultrasound intervention in cell growth according to an embodiment of the present invention includes: The electric heating chamber contains liquid and a perforated plate. The electric heating chamber is used to heat the liquid to a set temperature and maintain the set temperature constant. The liquid at the set temperature is used to heat multiple petri dishes placed on the perforated plate. The first moving component is connected to the bottom of the heating box and is adapted to move along the length of the heating box. The second moving component is connected to the first moving component and is adapted to move along the width direction of the electric heating box; An ultrasonic component includes a generator and a transducer. The generator is used to transmit electrical signals, and the transducer is electrically connected to the generator to convert the electrical signals into ultrasonic waves. The transducer is connected to the second moving component and located below the well plate. When the first and second moving components are working, they can drive the transducer to move between multiple culture dishes. The transducer is also connected to a connector assembly at its top, which points to the well plate and is used to focus the ultrasound waves emitted by the transducer onto the corresponding culture dish to perform ultrasound intervention on the cells in the culture dish.

[0009] Furthermore, the connector assembly includes a first fitting and a second fitting arranged coaxially: One end of the first tube is connected to the transducer, and the other end is axially movable to one end of the second tube. The other end of the second tube points towards the orifice plate. The first tube or the second tube can be moved axially, and the second tube can move closer to or further away from the orifice plate.

[0010] Furthermore, the end of the first pipe fitting connected to the transducer is provided with a connecting part, which is suitable for connecting different types of transducers; The connecting part includes: A connecting shell, located at the first end of the first pipe fitting, is a square shell with a bottom opening, and is connected to the first pipe fitting; The screw is rotatably connected to the connecting shell at both ends; The guide rod is parallel to the screw and its two ends are fixedly connected to the connecting shell; The clamping component is arc-shaped and comes in a quantity of 2. The two clamping parts are arranged in a mirror image, with their first end threadedly connected to the outer periphery of the screw and their second end slidably connected to the outer periphery of the guide rod; The transducer is placed between two clamping parts. By turning the screw, the two clamping parts move closer or further apart to clamp or loosen the transducer.

[0011] Furthermore, the second pipe fitting is provided with a pipe diameter adjustment section at its end near the orifice plate. The pipe diameter adjustment section includes: The flexible clamp is located at the end of the second pipe fitting and is shaped like a frustum. The adjusting ring is placed inside the elastic gripper. The tightening nut has a central hole, with one end of the hole wall having an internal thread and the other end having a tapered surface. The clamping nut is fitted onto the outer circumference of the elastic gripper and is connected to the end of the second pipe fitting via an internal thread. Tightening the clamping nut causes the conical surface to push the elastic gripper to compress the adjusting ring, so that the port diameter of the adjusting ring matches the diameter of the circular hole in the orifice plate.

[0012] Furthermore, the first moving component includes: The first lead screw extends along the length of the heating box, and its two ends are rotatably connected to the heating box and sealed to the heating box. The first slide rod is parallel to the first lead screw and extends along the length of the electric heating box. Both ends of the first slide rod are fixedly connected to the electric heating box and are respectively sealed to the electric heating box. The first slider has one side threadedly connected to the first lead screw and the other side slidably connected to the first slide rod; the second moving component is disposed on the first slider. Rotating the first lead screw causes the first slider to slide along the first lead screw and the first slide bar.

[0013] Furthermore, the second moving component includes: The second lead screw extends along the width of the heating box, and its two ends are rotatably connected to the first slider. The second slider is threaded to the outer periphery of the second lead screw, and its bottom is slidably connected to the first slider; The second slider is also equipped with a mounting base, and the transducer is connected to the mounting base; by rotating the second lead screw, the second slider can drive the transducer connected to the mounting base to move along the second lead screw.

[0014] Furthermore, the second leadscrew is driven by a drive assembly, which includes: A rotating seat is mounted on the first slider. The first bevel tooth is located at one end of the second lead screw; The second bevel tooth is located at the end of the rotating shaft and meshes with the first bevel tooth. The rotating shaft extends along the height of the heating box and is rotatably connected to the rotating seat. Twisting the rotating shaft drives the second bevel gear to mesh with the first bevel gear, thereby driving the second lead screw to rotate.

[0015] Furthermore, the mounting base includes: The first mounting component is located on the second slider; The second mounting component is connected to the first mounting component via a locking device; The first mounting component and the second mounting component are respectively provided with arc-shaped grooves, and the transducer is placed in the arc-shaped grooves of the first mounting component and the second mounting component; The screw-locking device allows the second mounting member to move toward or away from the first mounting member to clamp or release the transducer.

[0016] Furthermore, the locking device includes a locking rod and a spring; The first mounting part has a threaded hole, and the second mounting part has a through hole, with the threaded hole and the through hole communicating with each other. The locking lever passes through the through hole of the second mounting component and is screwed into the threaded hole of the first mounting component; The spring is a compression spring, which is fitted around the outer periphery of the locking rod. One end of the spring abuts against the second mounting piece, and the other end abuts against the end flange of the locking rod. Furthermore, both sides of the orifice plate along its length are connected to the housing via a third moving assembly, the third moving assembly comprising: The guide rail is located on the inner wall of the electric heating box and extends along the height of the electric heating box. The third slider is slidably connected to the guide rail. A third slide rod is vertically mounted on the slider, and a gripper is slidably connected to the third slide rod. The gripper is used to clamp the perforated plate.

[0017] One of the above technical solutions has at least the following advantages or beneficial effects: An experimental apparatus for assisting cell growth using ultrasound, according to an embodiment of this invention, comprises a heating chamber, a first moving component, a second moving component, and an ultrasound component. The heating chamber contains a liquid and well plates. The heating chamber heats the liquid to a set temperature and maintains that temperature. The liquid at the set temperature is used to heat the culture dishes on the well plates in a water bath, providing a suitable temperature for the cells and thus avoiding the impact of cell hypothermia on the experimental results.

[0018] The first and second moving components are located at the bottom of the heating chamber and are used to move the transducer of the ultrasound component between multiple culture dishes in the well plate. A connector assembly is provided at the top of the transducer. By adjusting the connector assembly, the ultrasound waves emitted by the transducer are focused onto the corresponding culture dish to perform ultrasound intervention on the cells within the culture dish, thereby understanding the therapeutic mechanism of ultrasound technology for clinical application. Attached Figure Description

[0019] Figure 1 A partial structural schematic diagram of an experimental device for assisting ultrasound intervention in cell growth according to an embodiment of the present invention is shown; Figure 2 This invention provides a schematic diagram of the structure of a first moving component, a second moving component, and a driving component in an experimental device for assisting ultrasound intervention in cell growth according to an embodiment of the present invention. Figure 3 This diagram shows a schematic representation of the drive component in an experimental apparatus for assisting ultrasound intervention in cell growth according to an embodiment of the present invention. Figure 4 This diagram shows a schematic of the locking device in an experimental apparatus for assisting ultrasound intervention in cell growth according to an embodiment of the present invention. Figure 5An exploded view of the connector assembly in an experimental apparatus for assisting ultrasound intervention in cell growth according to an embodiment of the present invention is shown. Figure 6 A schematic diagram of the structure of an experimental device for assisting ultrasound intervention in cell growth according to an embodiment of the present invention is shown.

[0020] [Explanation of Labels in the Attached Image] 10. Electric heating box; 20. First moving component; 21. First lead screw; 22. First slide bar; 23. First slider; 30. Second moving component; 31. Second lead screw; 32. Second slider; 33. Mounting base; 33-1. First mounting component; 33-2. Second mounting component; 34. Locking device; 34-1. Locking rod; 34-2. Spring; 40. Drive assembly; 41. Rotating seat; 42. Rotating shaft; 43. First bevel gear; 44. Second bevel gear; 50. Third moving component; 51. Guide rail; 52. Third slider; 53. Third sliding rod; 54. Gripper; 60. Connector assembly; 61. First fitting; 62. Second fitting; 63. Connecting part; 63-1. Connecting shell; 63-2. Screw; 63-3. Guide rod; 63-4. Clamping component; 64. Pipe diameter adjustment part; 64-1. Elastic clamp; 64-2. Adjusting ring; 64-3. Tightening nut; 70. Orifice plate; 80. Transducer. Detailed Implementation

[0021] To better explain and facilitate understanding of this utility model, the present utility model will be described in detail below with reference to the accompanying drawings and specific embodiments.

[0022] To address at least one of the technical problems existing in the prior art or related technologies, this invention provides an experimental device for assisting ultrasound intervention in cell growth, see [link to related document]. Figure 1 The experimental device includes an electric heating box 1, a first moving component 20, a second moving component 30, and an ultrasonic component.

[0023] A perforated plate 70 with multiple circular holes is placed inside the heating chamber 1. Each circular hole is used to hold a culture dish, and the chamber also holds liquid. The heating chamber 1 is used to heat the liquid to a set temperature and maintain the set temperature constant. At the set temperature, the liquid is used for water bath heating of the culture dishes. A first moving component 20 is located at the bottom of the heating chamber 1 and is adapted to move along the length of the heating chamber 1. A second moving component 30 is connected to the first moving component 20 and is adapted to move along the width of the heating chamber 1. The transducer 80 of the ultrasonic component is connected to the second moving component 30, and its top end is provided with a connector component 60 pointing towards the perforated plate 70. The connector component 60 is used to focus the ultrasonic waves emitted by the transducer 80 onto the corresponding culture dish to perform ultrasonic intervention on the cells in the culture dish. When the first moving component 20 and the second moving component 30 are working, they can drive the transducer 80 to move between the multiple circular holes of the perforated plate 70 to perform ultrasonic intervention on the culture dishes in the multiple circular holes respectively.

[0024] Here, the electric heating chamber 1 can heat the liquid placed inside and keep the temperature constant to provide a constant culture temperature for the cells, thereby eliminating the influence of temperature changes on the experimental results.

[0025] The electric heating box 1 is preferably an electric heating constant temperature water tank. The heating and heat preservation principle is the same as that of a constant temperature kettle, only the shape and size are different. Therefore, the specific structure of this embodiment is not described in detail.

[0026] In this embodiment, the ultrasonic component also includes a generator (not shown in the figure), which is used to emit electrical signals. The transducer 80 is electrically connected to the generator and is used to convert the electrical signals emitted by the generator into ultrasonic waves.

[0027] Since the required ultrasonic intensity varies, the frequency and diameter of the transducer 80 also vary. The far field and focal position of the sound waves of transducers 80 with different frequencies and diameters are different. In order to place the irradiated cells in this area to obtain a stable sound field and biological effect, a connector assembly 60 is set at the top of the transducer 80. The connector assembly 60 points to the well plate 70. By adjusting the connector assembly 60, the focal point of the ultrasonic wave is placed on the culture dish. This eliminates the need to repeatedly adjust the position of the transducer 80 and the well plate 70, making the operation easier.

[0028] See Figure 5 The connector assembly 60 includes a first tube 61 and a second tube 62 arranged coaxially. One end of the first tube 61 is connected to the transducer 80, and the other end is axially adjustable to the second tube 62. The second end of the second tube 62 points to the orifice plate 70. The first tube 61 or the second tube 62 can be moved axially to move closer to or further away from the orifice plate 70 to suit the usage requirements of different models of transducers 80.

[0029] For example, the first pipe fitting 61 and the second pipe fitting 62 are connected by threads. The first pipe fitting 61 is provided with an internal thread, and the second pipe fitting 62 is provided with an external thread. The second pipe fitting 62 is screwed onto the outer circumference of the internal thread of the first pipe fitting 61 through the external thread. By screwing the first pipe fitting 61 or the second pipe fitting 62, the axial adjustment of the first pipe fitting 61 and the second pipe fitting 62 can be achieved.

[0030] The first pipe fitting 61 is also provided with scale lines (not shown in the figure) in the extension direction. During axial adjustment, the end of the second pipe fitting 62 is aligned with the scale lines to accurately control the axial movement distance of the second pipe fitting 62.

[0031] Furthermore, the end of the first pipe 61 connected to the transducer 80 is also provided with a connecting part 63, which has a diameter adjustment function and can be adapted to the use requirements of transducers 80 with different diameters.

[0032] In an exemplary embodiment, the connecting part 63 includes a connecting shell 63-1, a screw 63-2, a guide rod 63-3, and a clamping member 63-4. The connecting shell 63-1 is located at the end of the first pipe 61 near the transducer 80. It is a square shell with an open bottom and is connected to the inner cavity of the first pipe 61. The two ends of the screw 63-2 are rotatably connected to the connecting shell 63-1. The guide rod 63-3 is parallel to the screw 63-2 and is fixedly connected to the connecting shell 63-1 at both ends. There are two clamping members 63-4. The two clamping members 63-4 are arc-shaped and arranged in a mirror image. One end of the clamping member 63-4 is threaded to the outer circumference of the screw 63-2, and the other end is slidably connected to the outer circumference of the guide rod 63-3. The transducer 80 is placed between the two clamping members 63-4. By screwing the screw 63-2, the two clamping members 63-4 move closer or further apart to clamp or release the transducer 80.

[0033] Understandably, the two arc-shaped clamping members 63-4, which are set in a mirror image, form a clamping structure. The distance between the two clamping members 63-4 can be adjusted by turning the screw 63-2 to adapt to the clamping requirements of transducers 80 with different diameters.

[0034] Furthermore, in this embodiment of the present disclosure, since different perforated plates 70 are selected for the experiment, such as 12-hole perforated plate 70, 9-hole perforated plate 70 or 6-hole perforated plate 70, the number of round holes and the hole diameters corresponding to different perforated plates 70 are different. In order to suit the use requirements of different hole diameters, the end of the second pipe fitting 62 near the perforated plate 70 is also provided with a pipe diameter adjustment part 64. The pipe diameter adjustment part 64 is used to adjust the opening diameter of the connector assembly 60 so that the opening diameter is adapted to the diameter of the round hole of the perforated plate 70.

[0035] In an exemplary embodiment, the pipe diameter adjustment part 64 includes an elastic gripper 64-1, an adjusting ring 64-2, and a clamping nut 64-3. The elastic gripper 64-1 is located at the end of the second pipe fitting 62 and is truncated conical in shape. The adjusting ring 64-2 is located within the inner ring of the elastic gripper 64-1. The clamping nut 64-3 has a central hole. One end of the clamping nut 64-3 has an internal thread, and the other end has a conical surface. The clamping nut 64-3 is fitted onto the outer circumference of the elastic gripper 64-1 and is threaded to the end of the second pipe fitting 62 via the internal thread. Tightening the clamping nut 64-3 causes the conical surface to push the elastic gripper 64-1 to compress the adjusting ring 64-2, so that the port diameter of the adjusting ring 64-2 matches the diameter of the circular hole on the orifice plate 70.

[0036] Here, the adjusting ring 64-2 is made of elastic materials such as rubber or silicone. When the tightening nut 64-3 is tightened, the conical surface of the tightening nut 64-3 pushes the frustoconical elastic claw 64-1 to converge inward, thereby compressing the adjusting ring 64-2 to adjust the port diameter. The adjustment range of the port diameter of the adjusting ring 64-2 is determined by the moving distance of the tightening nut 64-3 relative to the second pipe fitting 62. Its adjustment range is wide and can be adapted to the use requirements of different circular hole diameters of the orifice plate 70.

[0037] In this disclosure embodiment, see Figure 1 and Figure 2 The first moving component 20 includes a first lead screw 21, a first slide rod 22, and a first slider 23. The first lead screw 21 extends along the length of the heating chamber 1, with both ends rotatably connected to and sealed to the heating chamber 1. The first slide rod 22 is parallel to the first lead screw 21 and also extends along the length of the heating chamber 1, with both ends fixedly connected to and sealed to the heating chamber 1. The first slider 23 has a plate-like structure, extending in the same direction as the width of the heating chamber 1. One side of the first slider 23 is threadedly connected to the first lead screw 21, and the other side is slidably connected to the first slide rod 22. The second moving component 30 is mounted on the first slider 23.

[0038] In practical use, rotating the first lead screw 21 causes the first slider 23 to drive the second moving component 30, and the transducer 80 and connector component 60 connected to the second moving component 30 to slide along the extension direction of the first lead screw 21 and the first slide rod 22, so as to drive the transducer 80 and connector component 60 to move between multiple rows of round holes in the orifice plate 70.

[0039] Here, not shown in the figure, the two ends of the first lead screw 21 are rotatably connected to the heating box 1 through bearings, and the first lead screw 21 and the heating box 1 are dynamically sealed through oil seals. The two ends of the first slide rod 22 are statically sealed to the heating box 1 through sealing rings. This can ensure the sealing of the heating box 1 and prevent the liquid inside the heating box 1 from flowing out.

[0040] To facilitate the turning of the first lead screw 21, an internal hexagonal hole is provided at the end of the first lead screw 21. The first lead screw can be turned by rotating it through the external hexagonal hole outside the housing, which is convenient for operation.

[0041] In this disclosure embodiment, see Figure 2 The second moving component 30 includes a second lead screw 31 and a second slider 32. The second lead screw 31 extends along the width direction of the heating box 1, and its two ends are rotatably connected to the first slider 23. The second slider 32 is threaded to the outer periphery of the second lead screw 31, and its bottom is slidably connected to the first slider 23. The transducer 80 is connected to the second slider 32. Twisting the second lead screw 31 can drive the second slider 32 and the transducer 80 connected to the second slider 32 to move between multiple rows of circular holes in the orifice plate 70.

[0042] For example, hinge seats are provided on both sides of the first slider 23 along its length. The hinge seats and the first slider 23 are integrally formed. The two ends of the second lead screw 31 are rotatably connected to the hinge seats through bearings. A groove is also provided on the upper end surface of the first slider 23, and a slide rail is provided on the lower end surface of the second slider 32. The slide rail is slidably connected to the groove. When the second lead screw 31 rotates, it drives the slide rail on the second slider 32 to slide along the groove, thereby providing guidance and limiting for the movement of the second slider 32.

[0043] Furthermore, a mounting base 33 is also provided on the second slider 32, and the transducer 80 is detachably connected to the mounting base 33. The mounting base 33 can be adapted to the installation requirements of different types of transducers 80.

[0044] For example, see Figure 2 The mounting base 33 includes a first mounting member 33-1, a second mounting member 33-2, and a locking device 34. The first mounting member 33-1 is disposed on the second slider 32, and the second mounting member 33-2 is connected to the first mounting member 33-1 through the locking device 34. The first mounting member 33-1 and the second mounting member 33-2 are respectively provided with arc-shaped grooves. The transducer 80 is placed in the arc-shaped groove between the first mounting member 33-1 and the second mounting member 33-2. By turning the locking device 34, the second mounting member 33-2 can move towards or away from the first mounting member 33-1 to clamp or release the transducer 80.

[0045] It is understandable that the first mounting part 33-1 and the second mounting part 33-2 are used to fix the transducer 80 by clamping. The locking device 34 provides clamping force for the transducer 80. The distance between the arc centers of the arc grooves of the first mounting part 33-1 and the second mounting part 33-2 is adjusted by the locking device 34 to meet the clamping requirements of different types of transducers 80.

[0046] For example, see Figure 4 There are two locking devices 34 arranged symmetrically. Each locking device 34 includes a locking rod 34-1 and a spring 34-2. Correspondingly, the first mounting part 33-1 has two threaded holes symmetrically arranged, and the second mounting part 33-2 has two through holes symmetrically arranged, which are connected to the threaded holes. The locking rods 34-1 of the two locking devices 34 pass through the two through holes on the second mounting part 33-2 and are threadedly connected to the two threaded holes on the first mounting part 33-1. The spring 34-2 is fitted around the outer periphery of the locking rod 34-1, with one end abutting against the second mounting part 33-2 and the other end abutting against the flange at the end of the locking rod 34-1.

[0047] Here, spring 34-2 is a compression spring. When clamping the transducer 80, turning the locking rod 34-1 compresses the spring 34-2, generating spring force. This spring force pushes the second mounting piece 33-2 to clamp the transducer 80, which is placed between the first mounting piece 33-1 and the second mounting piece 33-2. When it is necessary to replace the transducer 80, rotating the locking rod 34-1 in the opposite direction resets the spring 34-2. This allows the second mounting piece 33-2 to be moved away from the first mounting piece 33-1, enabling the transducer 80 to be disassembled and replaced. This method is convenient, quick, and suitable for the needs of different types of transducers 80, making it widely applicable.

[0048] In this embodiment of the disclosure, due to the space limitation of the electric heating box 1, it is not convenient to turn the second lead screw. Therefore, a drive assembly 40 is specially provided. The drive assembly 40 is connected to the second lead screw 31 and is used to drive the second lead screw 31 to rotate.

[0049] For example, see Figure 3 The drive assembly 40 includes a rotating seat 41, a rotating shaft 42, a first bevel gear 43, and a second bevel gear 44. The rotating seat 41 is mounted on the first slider 23. The rotating shaft 42 extends along the height direction of the heating box 1 and is rotatably connected to the rotating seat 41 via bearings. The first bevel gear 43 is located at the end of the second lead screw 31, and the second bevel gear 44 is located at one end of the rotating shaft 42 and meshes with the first bevel gear 43. A handwheel is also provided at the other end of the rotating shaft 42. The rotary handwheel drives the rotating shaft 42 and the second bevel gear 44 located at the end of the rotating shaft 42 to rotate synchronously. The second bevel gear 44 meshes with the first bevel gear 43 to drive the second lead screw 31 to rotate.

[0050] It is understood that by setting the drive component 40, the second lead screw 31 can be driven to rotate outside the heating box 1, which effectively solves the technical problem that the second lead screw 31 is not easy to adjust due to the small size of the heating box 1.

[0051] Further, see Figure 1 and Figure 6The experimental device for assisting cell growth with ultrasound in this embodiment also includes a third moving component 50. There are two third moving components 50, which are respectively disposed on the walls of the heating box 1 on both sides along the length direction. The two sides of the perforated plate 70 along the length direction are respectively connected to the two third moving components 50. The two third moving components 50 are adapted to drive the perforated plate 70 to be immersed in or removed from the liquid.

[0052] For example, the third moving component 50 includes a guide rail 51 and a third slider 52. The guide rail 51 is disposed on the inner wall of the heating box 1 and extends along the height direction of the heating box 1. The third slider 52 is slidably connected to the guide rail 51, and the third slider 52 can be pushed to slide along the extension direction of the guide rail 51.

[0053] The third slider 52 is also vertically provided with a third slide rod 53, and a gripper 54 is slidably connected to the third slide rod 53. By pushing the gripper 54 to slide along the third slide rod 53, the distance between the two grippers 54 can be adjusted to suit the use requirements of orifice plates 70 of different sizes.

[0054] It is understood that the guide rail 51 and the heating box 1, and the third slide rod 53 and the third slider 52 can be an integral structure or separate structures. When it is a separate structure, the connection method can adopt any suitable form in the prior art, such as welding, snap-fitting, bonding or bolting, etc., but this embodiment does not specifically limit its fixing method.

[0055] It should be further explained that the cross-sectional shape of the third slide bar 53 is square, polygonal or any irregular structure that is not easy to rotate. The structure of the connection between the gripper 54 and the third slide bar 53 is adapted to the third slide bar 53. During use, it can prevent the gripper 54 from twisting and can effectively avoid the occurrence of the culture dish tilting and leakage on the well plate 70 due to the rotation of the gripper 54.

[0056] The gripper 54 can be a clamp-type gripper 54 or a rectangular block with an opening as shown in the figure. The gripper 54 can be selected according to the usage requirements and manufacturing costs. This embodiment does not specifically limit its structure.

[0057] Before or after the experiment, the third slider 52 can be pushed along the guide rail 51 to move the gripper 54 and the orifice plate 70 clamped on the gripper 54 into or out of the liquid, facilitating operation. When in the liquid, the gripper 54 can also limit the orifice plate 70, ensuring that the orifice plate 70 is completely immersed in the liquid, preventing the orifice plate 70 from floating due to liquid buoyancy and causing uneven heating of the culture dish, thus avoiding the impact of uneven heating on the accuracy and authenticity of the experimental results.

[0058] The above describes the specific structure of the experimental device for assisting cell growth with ultrasound in this embodiment. Before the experiment, the transducer 80 is clamped onto the mounting base 33. The height and opening diameter of the connector assembly 60 are adjusted according to the type of transducer 80 and well plate 70 to accommodate their dimensions. Then, liquid is added to the heating chamber 1, and the heating and temperature control functions of the heating chamber 1 are activated to maintain the liquid temperature at the set value. Next, the well plate 70 containing the culture dish is clamped onto the third moving assembly 50, which then moves the well plate. The plate 70 moves along the height of the heating box 1 until it is completely immersed in the liquid. Then, the transducer 80 and connector assembly 60 are moved by the first moving component 20 and the second moving component 30 so that the connector assembly 60 is exactly pointing to a certain round hole on the plate 70. Finally, the generator is turned on to start the ultrasonic intervention on the culture dish. After the set time is reached, the transducer 80 and connector assembly 60 are adjusted to the position of the next round hole by the first moving component 20 and the second moving component 30, so as to complete the cell intervention in all the round holes of the culture dish in sequence.

[0059] During the experiment, the culture dishes were completely immersed in a constant-temperature liquid, and the cells did not experience temperature loss even after prolonged ultrasound exposure, thus facilitating the objective analysis of the effects of ultrasound on cell growth. Secondly, by setting up the first moving component 20 and the second moving component 30, the positions of the transducer 80 and the connector component 60 can be adjusted externally. Multiple culture dishes can be assembled and disassembled in a single setup to complete the ultrasound intervention experiment, eliminating the need for repeated assembly and disassembly, making it convenient and quick. Thirdly, the connector component 60 can focus the ultrasound waves onto the corresponding culture dish to provide sufficient ultrasound stimulation to the cells within, allowing for the exploration of the therapeutic mechanisms of ultrasound technology in the laboratory stage, facilitating clinical application.

[0060] In the description of this utility model, it should be understood that the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this utility model, "a plurality of" means two or more, unless otherwise explicitly specified.

[0061] In this utility model, unless otherwise explicitly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the connection of components within two elements or the interaction between two elements. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0062] In this utility model, unless otherwise explicitly specified and limited, "above" or "below" the second feature can mean that the first feature is in direct contact with the second feature, or that the first feature is in indirect contact with the second feature through an intermediate medium. Furthermore, "above," "on top of," and "over" the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "beneath" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0063] In the description of this specification, the terms "one embodiment," "some embodiments," "embodiment," "exemplary embodiment," "example," "specific example," or "some examples," etc., refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

[0064] Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those skilled in the art can make modifications, alterations, substitutions and variations to the above embodiments within the scope of the present invention.

Claims

1. An experimental apparatus for assisting ultrasound in intervening in cell growth, characterized in that, include: An electric heating chamber contains a liquid and a perforated plate. The electric heating chamber is used to heat the liquid to a set temperature and maintain the set temperature constant. The liquid at the set temperature is used to heat multiple petri dishes placed on the perforated plate. A first movable component is connected to the bottom of the electric heating box and is adapted to move along the length of the electric heating box. The second moving component is connected to the first moving component and is adapted to move along the width direction of the electric heating box; An ultrasonic component includes a generator and a transducer, wherein the generator is used to transmit an electrical signal, and the transducer is electrically connected to the generator for converting the electrical signal into ultrasonic waves; The transducer is connected to the second moving component and located below the well plate. When the first moving component and the second moving component are working, they can drive the transducer to move between multiple culture dishes. The transducer is also connected to a connector assembly at its top, which points to the well plate and is used to focus the ultrasonic waves emitted by the transducer onto the corresponding culture dish to perform ultrasonic intervention on the cells in the culture dish.

2. The experimental apparatus for assisting cell growth with ultrasound as described in claim 1, characterized in that, The connector assembly includes a first and a second pipe fitting arranged coaxially: One end of the first tube is connected to the transducer, and the other end is axially movably connected to one end of the second tube. The other end of the second tube points to the orifice plate. The first tube or the second tube can be moved axially, and the second tube can move towards or away from the orifice plate.

3. The experimental apparatus for assisting cell growth with ultrasound as described in claim 2, characterized in that, The end of the first pipe fitting connected to the transducer is provided with a connecting part, which is adapted to connect different types of transducers; The connecting part includes: A connecting shell, located at the first end of the first pipe fitting, is a square shell with a bottom opening, and the connecting shell is connected to the first pipe fitting; The screw is rotatably connected to the connecting shell at both ends; A guide rod, parallel to the screw, is fixedly connected at both ends to the connecting shell; The clamping component is arc-shaped and comes in a quantity of 2. The two clamping members are arranged in a mirror image, with their first end threadedly connected to the outer periphery of the screw and their second end slidably connected to the outer periphery of the guide rod; The transducer is placed between the two clamping members. By screwing the screw, the two clamping members move closer or further apart to clamp or loosen the transducer.

4. The experimental apparatus for assisting cell growth with ultrasound as described in claim 2, characterized in that, The second pipe fitting is further provided with a pipe diameter adjustment part at its end near the orifice plate, the pipe diameter adjustment part comprising: The elastic gripper is located at the end of the second pipe fitting and is shaped like a frustum. The adjusting ring is located within the inner ring of the elastic gripper; A clamping nut is provided with a central hole, one end of the hole wall of the clamping nut has an internal thread, and the other end of the hole wall has a conical surface; The clamping nut is sleeved on the outer periphery of the elastic gripper and is connected to the end of the second pipe fitting through an internal thread. When the clamping nut is screwed on, the conical surface pushes the elastic gripper to compress the adjusting ring so that the port diameter of the adjusting ring matches the diameter of the circular hole in the orifice plate.

5. The experimental apparatus for assisting cell growth with ultrasound as described in claim 1, characterized in that, The first moving component includes: The first lead screw extends along the length of the electric heating box, and its two ends are rotatably connected to the electric heating box and are respectively sealed to the electric heating box. The first slide rod is parallel to the first lead screw and extends along the length of the electric heating box. Both ends of the first slide rod are fixedly connected to the electric heating box and are respectively sealed to the electric heating box. The first slider has one side threadedly connected to the first lead screw and the other side slidably connected to the first slide rod; the second moving component is disposed on the first slider. Rotating the first lead screw causes the first slider to slide along the first lead screw and the first slide bar.

6. The experimental apparatus for assisting cell growth with ultrasound as described in claim 5, characterized in that, The second moving component includes: The second lead screw extends along the width of the heating box, and its two ends are rotatably connected to the first slider. The second slider is threaded to the outer periphery of the second lead screw, and its bottom is slidably connected to the first slider; The second slider is also provided with a mounting base, and the transducer is connected to the mounting base; by rotating the second lead screw, the second slider can drive the transducer connected to the mounting base to move along the second lead screw.

7. The experimental apparatus for assisting cell growth with ultrasound as described in claim 6, characterized in that, The second leadscrew is driven by a drive assembly, the drive assembly comprising: A rotating seat is mounted on the first slider. The first bevel tooth is located at one end of the second lead screw; The second bevel tooth is located at the end of the rotating shaft and meshes with the first bevel tooth. The rotating shaft extends along the height direction of the electric heating box and is rotatably connected to the rotating seat. Twisting the rotating shaft is used to drive the second bevel tooth to mesh with the first bevel tooth, thereby driving the second lead screw to rotate.

8. The experimental apparatus for assisting cell growth with ultrasound as described in claim 6, characterized in that, The mounting base includes: The first mounting component is disposed on the second slider; The second mounting component is connected to the first mounting component via a locking device; The first mounting component and the second mounting component are respectively provided with arc-shaped grooves, and the transducer is placed in the arc-shaped grooves of the first mounting component and the second mounting component; Tightening the locking device allows the second mounting member to move toward or away from the first mounting member to clamp or loosen the transducer.

9. The experimental apparatus for assisting cell growth with ultrasound as described in claim 8, characterized in that, The locking device includes a locking rod and a spring; The first mounting component has a threaded hole, and the second mounting component has a through hole, wherein the threaded hole and the through hole communicate with each other; The locking rod passes through the through hole of the second mounting member and is screwed into the threaded hole of the first mounting member; The spring is a compression spring, which is fitted around the outer periphery of the locking rod. One end of the spring abuts against the second mounting piece, and the other end abuts against the end flange of the locking rod.

10. The experimental apparatus for intervening in cell growth with assisted ultrasound as described in any one of claims 1 to 9, characterized in that, The two sides of the perforated plate along its length are respectively connected to the housing via a third movable component, the third movable component comprising: A guide rail is provided on the inner wall of the electric heating box, and the guide rail extends along the height direction of the electric heating box. The third slider is slidably connected to the guide rail. A third slide rod is vertically arranged on the slider, and a gripper is slidably connected to the third slide rod. The gripper is used to clamp the perforated plate.

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