A device for pretreatment of protein precipitation in biological detection samples

Abstract

This utility model discloses a pretreatment device for protein precipitation in biological detection samples, including a protein precipitation pretreatment plate. A filter plate cover is snapped onto the top of the protein precipitation pretreatment plate, and a limiting mechanism is fixedly connected to the lower surface of the protein precipitation pretreatment plate. A 96-well sample loading plate is slidably connected to the inner side of the limiting mechanism. In this utility model, the 96-well pretreatment plate device has a simple structure design and is easy to manufacture due to the protein precipitation pretreatment plate and limiting mechanism. Furthermore, for the separation and collection of extracts after protein precipitation, the extract collection step can be performed in batches, saving pretreatment time, avoiding extract spillage and cross-contamination, simplifying operation, enhancing the repeatability of the collection process, avoiding operator error during pretreatment, saving sample detection pretreatment time, improving pretreatment efficiency, and making it more practical.

Description

Technical Field

[0001] This utility model relates to the field of biological detection equipment technology, and in particular to a device for pretreatment of protein precipitation in biological detection samples. Background Technology

[0002] When the obtained sample cannot directly meet the detection requirements, such as due to the presence of particles, interfering substances, or insufficient concentration, certain pretreatment methods are necessary. Currently, most 96-well pretreatment plates on the market are ordinary plates. However, when used for protein precipitation technology, the supernatant needs to be aspirated and processed after sample precipitation. There are also commercially available filter plates specifically designed for protein precipitation. After protein precipitation, the liquid is forced through the filter medium using negative pressure or centrifugation. The precipitated protein is retained above the filter medium, while the clarified liquid passes through the filter membrane into the collection plate below, thus achieving the separation of protein from the target solution.

[0003] Currently, in the pretreatment process of protein filtration plates, the steps for collecting the extract under negative pressure or by centrifugation are not perfectly aligned with the process of the extract passing through the filter membrane and into the collection plate below. This can lead to liquid spillage and cross-contamination, affecting the accuracy of the results. Furthermore, existing 96-well plates are ordinary pretreatment filter plates without dedicated collection devices. Daily operations require the addition of filter and collection plates for fixation, which is cumbersome. Manual fixation can result in inconsistent fixation effects, impacting the convenience of experimental operations and the repeatability and accuracy of experimental results, thus presenting certain shortcomings. Utility Model Content

[0004] The purpose of this invention is to address the shortcomings of existing technologies by proposing a pretreatment device for protein precipitation in biological detection samples.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: a pretreatment device for protein precipitation in biological detection samples, comprising a protein precipitation pretreatment plate, a filter plate cover being snapped onto the top of the protein precipitation pretreatment plate, a limiting mechanism being fixedly connected to the lower surface of the protein precipitation pretreatment plate, and a 96-well sample plate being slidably connected to the inner side of the limiting mechanism.

[0006] Furthermore, the protein precipitation pretreatment plate includes a filter plate frame, the filter plate frame having a plurality of processing chambers inside, and each of the plurality of processing chambers having a microporous filter plate fixedly connected inside.

[0007] Furthermore, the limiting mechanism includes a top plate, the upper surface of which is fixedly connected to the bottom of the filter plate frame, and side plates fixedly connected to the front and rear sides of the lower surface of the top plate. The bottom of each of the two side plates is fixedly connected to a bottom plate, and one end of each of the two side plates is fixedly connected to the same limiting plate.

[0008] Furthermore, the top of the limiting plate is fixedly connected to one end of the lower surface of the top plate, and the two side plates and the single limiting plate together form a U-shaped structure.

[0009] Furthermore, the 96-hole upper template has multiple cavities inside that correspond to the processing chambers, and the top of the 96-hole upper template is in close contact with the top plate.

[0010] Furthermore, the sidewall of the top plate is provided with several through holes that match the processing chamber, and the diameter of the through holes is smaller than the diameter of the microporous filter plate.

[0011] This utility model has the following beneficial effects:

[0012] In use, this utility model provides a protein precipitation pretreatment device for biological detection samples. Through its protein precipitation pretreatment plate and limiting mechanism, the 96-well pretreatment plate device features a simple structure that is easy to manufacture. Furthermore, for the separation and collection of the extract after protein precipitation, the extract collection step can be performed in batches, saving pretreatment time, preventing spillage and cross-contamination, simplifying operation, enhancing the repeatability of the collection process, reducing operator error rates during pretreatment, saving sample pretreatment time, improving operational efficiency, and making it more practical. Attached Figure Description

[0013] Figure 1 This is a top view of the protein precipitation pretreatment plate of this utility model;

[0014] Figure 2 This is a front sectional view of the overall structure of this utility model;

[0015] Figure 3 This is a side sectional view of the overall structure of this utility model;

[0016] Figure 4 This is a perspective view of the limiting mechanism of this utility model;

[0017] Figure 5 This is a flowchart illustrating the usage state of this utility model.

[0018] Legend:

[0019] 1. Protein precipitation pretreatment plate; 101. Filter plate holder; 102. Microporous filter plate; 2. Processing chamber; 3. Filter plate cover; 4. Limiting mechanism; 401. Top plate; 402. Side plate; 403. Limiting plate; 404. Bottom plate; 5. 96-well sample loading plate; a. Protein precipitation pretreatment device assembly state; b. Protein precipitation pretreatment sample loading and mixing state; c. Protein precipitation pretreatment after centrifugation state. Detailed Implementation

[0020] Figures 1 to 5 As shown, this relates to a pretreatment device for protein precipitation in biological detection samples, including a protein precipitation pretreatment plate 1. A filter plate cover 3 is snapped onto the top of the protein precipitation pretreatment plate 1. A limiting mechanism 4 is fixedly connected to the lower surface of the protein precipitation pretreatment plate 1, and a 96-well sample plate 5 is slidably connected to the inner side of the limiting mechanism 4. The 96-well sample plate 5 for collecting the extract can be placed into the limiting mechanism 4 below the 96-well protein precipitation pretreatment plate 1 from the side. After adding precipitant, extract, and sample and mixing, centrifugation is performed. The 96-well sample plate containing the extract can be taken out from the bottom side for instrument detection.

[0021] The protein precipitation pretreatment plate 1 includes a filter plate frame 101. The filter plate frame 101 has several processing chambers 2 inside. Each of the processing chambers 2 is fixedly connected to a microporous filter plate 102. The side wall of the top plate 401 has several through holes that match the processing chambers 2, and the diameter of the through holes is smaller than the diameter of the microporous filter plate 102.

[0022] The limiting mechanism 4 includes a top plate 401, the upper surface of which is fixedly connected to the bottom of the filter plate holder 101. Side plates 402 are fixedly connected to the front and rear sides of the lower surface of the top plate 401. A bottom plate 404 is fixedly connected to the bottom of each of the two side plates 402, and a limiting plate 403 is fixedly connected to one end of each of the two side plates 402. The top of the limiting plate 403 is fixedly connected to one end of the lower surface of the top plate 401. The two side plates 402 and the single limiting plate 403 together form a U-shaped structure, which allows the 96-hole upper sample plate 5 to move back and forth, making it easy to remove or place inside the limiting mechanism 4. The interior of the 96-hole upper sample plate 5 has multiple cavities corresponding to the processing chamber 2, and the top of the 96-hole upper sample plate 5 is in close contact with the top plate 401.

[0023] In use, this invention offers several advantages. First, the 96-well pretreatment plate device has a simple structure and is easy to manufacture. The 96-well plate used is a commonly used laboratory consumable, inexpensive and readily available. For the separation and collection of extracts after protein precipitation, it avoids spillage and cross-contamination, simplifies operation, enhances the repeatability of the collection process, reduces operator error during pretreatment, saves sample pretreatment time, and improves operational efficiency.

[0024] Specifically, firstly, the 96-well sample collection plate 5 is installed from the side into the limiting mechanism 4 below the 96-well protein precipitation pretreatment plate 1. After the device is installed, it is presented... Figure 5 The state of 'a';

[0025] Second, protein precipitant and specimens are added to several processing chambers 2 inside the filter plate holder 101, sealed with a 96-well sealing membrane, and the filter plate cover 3 is closed. After vortexing and centrifugation, the samples are presented. Figure 5 The state of b in the middle;

[0026] Third, after centrifugation, it presents... Figure 5 In the state of c, the extract is collected into the 96-well sample plate 5 below. The 96-well sample plate 5 containing the extract is pulled out from the side. The supernatant of the 96-well sample plate 5 below, which has been centrifuged, is taken out for instrument testing. At the same time, the 96-well filter plate after protein precipitation pretreatment is disposed of as medical waste.

[0027] In summary, this device allows operators to conveniently perform protein precipitation pretreatment and collect the extract.

[0028] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A device for pretreatment of protein precipitation in biological detection samples, comprising a protein precipitation pretreatment plate (1), characterized in that: The top of the protein precipitation pretreatment plate (1) is snapped with a filter plate cover (3), the lower surface of the protein precipitation pretreatment plate (1) is fixedly connected with a limiting mechanism (4), and the inner side of the limiting mechanism (4) is slidably connected with a 96-hole sample plate (5).

2. The apparatus for pretreatment of protein precipitation in biological detection samples according to claim 1, characterized in that: The protein precipitation pretreatment plate (1) includes a filter plate frame (101), and the filter plate frame (101) has several processing chambers (2) inside, and microporous filter plates (102) are fixedly connected inside the several processing chambers (2).

3. The apparatus for pretreatment of protein precipitation in biological detection samples according to claim 2, characterized in that: The limiting mechanism (4) includes a top plate (401), the upper surface of which is fixedly connected to the bottom of the filter plate frame (101), and side plates (402) are fixedly connected to the front and rear sides of the lower surface of the top plate (401). The bottom of the two side plates (402) is fixedly connected to a bottom plate (404), and one end of the two side plates (402) is fixedly connected to the same limiting plate (403).

4. The apparatus for pretreatment of protein precipitation in biological detection samples according to claim 3, characterized in that: The top of the limiting plate (403) is fixedly connected to one end of the lower surface of the top plate (401), and the two side plates (402) and the single limiting plate (403) together form a U-shaped structure.

5. The apparatus for pretreatment of protein precipitation in biological detection samples according to claim 4, characterized in that: The 96-hole upper template (5) has multiple cavities inside that correspond to the processing chamber (2), and the top of the 96-hole upper template (5) is in close contact with the top plate (401).

6. The apparatus for pretreatment of protein precipitation in biological detection samples according to claim 5, characterized in that: The top plate (401) has several through holes on its sidewall that match the processing chamber (2), and the diameter of the through holes is smaller than the diameter of the microporous filter plate (102).

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