Chromatography column for protein purification

Abstract

This utility model discloses a chromatography column for protein purification, relating to the field of protein purification technology. It includes a chromatography column body made of transparent material with an open top. An injection mechanism includes a top cap, an annular cover, an injection cylinder, a piston block, and a push-pull rod. The bottom of the top cap is fixedly connected to the top of the annular cover, and the bottom of the top cap contacts the edge of the opening at the top of the chromatography column body. The size of the top cap is larger than the top size of the chromatography column body. The inner wall of the annular cover is tightly fitted to the outer wall of the chromatography column body. The bottom end of the injection cylinder extends through the top cap into the interior of the chromatography column body and is rotatably connected to the top cap. Multiple injection tubes are provided at the bottom of the injection cylinder. This utility model can greatly improve the mixing efficiency and uniformity of the dissolving solution or neutral salt solution with the protein purification stock solution, thereby significantly improving its practicality.

Description

Technical Field

[0001] This utility model relates to the field of protein purification technology, and in particular to a chromatography column for protein purification. Background Technology

[0002] Protein isolation and purification are widely used in biochemical research and are an important operational technique. A typical eukaryotic cell can contain thousands of different proteins, some abundantly and others containing only a few copies. To study a particular protein, it must first be purified from other protein and non-protein molecules. Chromatographic columns are generally used in protein purification processes, as shown below.

[0003] A search revealed a patent with authorization announcement number CN219502026U, which discloses a protein purification chromatography column. The column includes a column body with a cover plate at the top and graduated strips on its outer circumference. A rubber ring is installed inside the cover plate, and an injection body is installed inside the rubber ring. An injection port is located on the outer circumference of the injection body, and a support block is installed at its top. A scale is located on the outer circumference of the injection body away from the injection port. A circular column is installed inside the injection body, with a connecting rod at its top. A circular plate is installed at the top of the connecting rod, and an injection port is located inside the circular plate. A plug is installed at the top of the injection port. This device facilitates the control of the amount of stock solution and dissolving solution added. Multiple injection ports ensure that the dissolving solution and neutral salt are fully and evenly integrated into the stock solution, facilitating the complete mixing of the neutral salt and the raw material, thereby improving protein purification efficiency. It also allows for the extraction of the stock solution from inside the chromatography column.

[0004] However, the above-mentioned protein purification chromatography column still has the following areas for optimization. For example, although theoretically, by pulling out the circular column inside the injector nozzle, the dissolving solution or neutral salt solution inside the injector can be uniformly integrated into the protein stock solution, and then the injector can be removed and the top of the chromatography column body can be sealed, it is possible to facilitate the full integration of the dissolving solution or neutral salt solution with the raw material, thereby improving the protein purification efficiency, in actual work, after pulling out the circular column, although the dissolving solution or neutral salt solution can mix with the protein stock solution, it can only rely on the two to mix naturally, resulting in a slow mixing speed and generally poor mixing uniformity. Therefore, its structure needs to be improved and its practicality needs to be enhanced. Utility Model Content

[0005] This utility model discloses a chromatography column for protein purification. It is equipped with an injection mechanism and an auxiliary stirring mechanism, allowing the syringe to be directly removed during operation, along with the top cap. The protein purification stock solution can then be added to the inside of the chromatography column. After adding the stock solution, a certain amount of dissolving solution or neutral salt solution is drawn into the syringe through multiple injection tubes via the syringe, piston block, and push-pull rod. The syringe is then inserted into the chromatography column until the bottom of the top cap is in contact with the top of the column. The top cap can be used with an annular cover to... The top opening of the chromatography column body is sealed to prevent impurities from entering the chromatography column body. Then, the push-pull rod can be pressed, which, together with the piston block, delivers the dissolving solution or neutral salt solution in the syringe into the chromatography column body through the injection tube and multiple injection holes by squeezing pressure until the piston block is pushed to the bottom of the syringe. This allows the dissolving solution or neutral salt solution to be injected directly into the protein purification stock solution for mixing, rather than being slowly mixed naturally. This can initially accelerate the mixing efficiency of the dissolving solution or neutral salt solution with the protein purification stock solution and initially improve the mixing uniformity.

[0006] Furthermore, by using two rotating plates to rotate the syringe, the rotating shaft at the bottom of the syringe and multiple sets of stirring rods can be rotated slowly, thereby further accelerating the mixing efficiency of the dissolving solution or neutral salt solution with the protein purification stock solution and further improving the mixing uniformity. In summary, this can greatly improve the mixing efficiency and mixing uniformity of the dissolving solution or neutral salt solution with the protein purification stock solution, thus solving the problems in the background technology.

[0007] To solve the above-mentioned technical problems, this utility model is achieved through the following technical solution:

[0008] This utility model discloses a chromatography column for protein purification, including a chromatography column body, the chromatography column body being made of a transparent material, and the top of the chromatography column body being an open design;

[0009] An injection mechanism includes a top cover, an annular cover, an injection cylinder, a piston block, and a push-pull rod. The bottom of the top cover is fixedly connected to the top of the annular cover, and the bottom of the top cover contacts the top opening edge of the chromatography column body. The size of the top cover is larger than the top size of the chromatography column body. The inner wall of the annular cover is tightly fitted to the outer wall of the chromatography column body. The bottom end of the injection cylinder extends through the top cover into the interior of the chromatography column body, and the injection cylinder is rotatably connected to the top cover. The bottom of the injection cylinder is provided with multiple injection tubes, and the outer side of each injection tube is provided with multiple through injection holes. The piston block is located inside the injection cylinder and is tightly fitted to the inner wall of the injection cylinder. The bottom end of the push-pull rod is fixedly connected to the top end of the piston block.

[0010] An auxiliary stirring mechanism is provided, comprising a rotating shaft, stirring rods, and rotating plates. The top end of the rotating shaft is fixedly connected to the center point of the bottom of the injection cylinder. Multiple sets of stirring rods are provided on the outer circumference of the rotating shaft, with each set of stirring rods located between two adjacent injection tubes. Two rotating plates are provided, each fixedly connected to one or both sides of the injection cylinder, and the rotating plates are located close to the top of the injection cylinder.

[0011] Furthermore, the outer side of the chromatography column body is provided with a first graduation line, and the outer side of the injection cylinder is provided with a second graduation line.

[0012] Furthermore, the multiple injection tubes are arranged in a ring array, and the injection tubes and the injection cylinder are integrally molded.

[0013] Furthermore, multiple support bars are fixedly connected to the outer side of the push-pull rod, and the support bars are in contact with the inner wall of the injection cylinder.

[0014] Furthermore, the inner wall of the annular cover is a smooth surface, and the side of the support strip that contacts the injection cylinder is also a smooth surface.

[0015] Furthermore, a sealing gasket layer is provided at the bottom of the top cover, and the part of the syringe that penetrates the top cover is sealed.

[0016] The present invention has the following advantages over the prior art:

[0017] 1. This technical solution, by incorporating an injection mechanism and an auxiliary stirring mechanism, allows for direct removal of the injection mechanism during operation. The protein purification stock solution is then added to the chromatography column. After addition, the injection mechanism draws a certain amount of dissolving solution or neutral salt solution, inserts the injection mechanism into the chromatography column, and uses extrusion pressure to deliver the dissolving solution or neutral salt solution directly into the protein purification stock solution for mixing, rather than slow natural mixing. This initially accelerates the mixing efficiency of the dissolving solution or neutral salt solution with the protein purification stock solution and improves the mixing uniformity. The auxiliary stirring mechanism further accelerates the mixing efficiency and improves the mixing uniformity. In summary, this solution significantly improves the mixing efficiency and uniformity of the dissolving solution or neutral salt solution with the protein purification stock solution, thereby greatly enhancing its practicality.

[0018] 2. This technical solution incorporates an injection mechanism that ensures the piston block remains at the bottom of the syringe after mixing the dissolving solution or neutral salt solution with the protein purification stock solution. The syringe can then be easily removed. The piston block prevents the mixed solution from entering the syringe during removal, effectively preventing residual solution from being carried out and thus avoiding any impact on protein purification. This further enhances its practicality. Attached Figure Description

[0019] To more clearly illustrate the technical solutions of the embodiments of this utility model, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0020] Figure 1 This is a schematic diagram of the overall three-dimensional structure of this utility model;

[0021] Figure 2 This is a schematic diagram of the exploded structure of the injection cartridge of this utility model;

[0022] Figure 3 An exploded view of the piston block installation of this utility model;

[0023] Figure 4 This is a schematic diagram of the support bar installation structure of this utility model;

[0024] Figure 5 This is a schematic diagram of the exploded structure of the stirring rod installation of this utility model.

[0025] In the diagram: 1. Chromatography column body; 2. Injection mechanism; 201. Top cover; 202. Annular cover; 203. Injection cylinder; 204. Piston block; 205. Push-pull rod; 206. Injection tube; 207. Injection hole; 208. Second graduation line; 209. Support bar; 3. Auxiliary stirring mechanism; 301. Rotating shaft; 302. Stirring rod; 303. Rotating plate; 4. First graduation line. Detailed Implementation

[0026] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present utility model.

[0027] In the description of this utility model, it should be understood that the terms "surface", "side", "gap", "peripheral", etc., which indicate orientation or positional relationship, are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the components or elements referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model.

[0028] Reference Figures 1-5 A chromatography column for protein purification includes a chromatography column body 1, which is made of a transparent material and has an open top.

[0029] The injection mechanism 2 includes a top cover 201, an annular cover 202, an injection cylinder 203, a piston block 204, and a push-pull rod 205. The bottom of the top cover 201 is fixedly connected to the top of the annular cover 202. The bottom of the top cover 201 contacts the top opening edge of the chromatography column body 1. The size of the top cover 201 is larger than the top size of the chromatography column body 1. The inner wall of the annular cover 202 is tightly fitted to the outer wall of the chromatography column body 1. The bottom end of the injection cylinder 203 extends through the top cover 201 into the interior of the chromatography column body 1. The injection cylinder 203 is rotatably connected to the top cover 201. The bottom of the injection cylinder 203 is provided with multiple injection tubes 206. The outer side of the injection tubes 206 is provided with multiple through injection holes 207. The piston block 204 is located inside the injection cylinder 203 and is tightly fitted to the inner wall of the injection cylinder 203. The bottom end of the push-pull rod 205 is fixedly connected to the top end of the piston block 204.

[0030] The auxiliary stirring mechanism 3 includes a rotating shaft 301, stirring rods 302, and a rotating plate 303. The top end of the rotating shaft 301 is fixedly connected to the center point of the bottom of the injection cylinder 203. Multiple sets of stirring rods 302 are provided on the outer circumference of the rotating shaft 301. Each set of stirring rods 302 is located between two adjacent injection tubes 206. Two rotating plates 303 are provided. The two rotating plates 303 are fixedly connected to the two sides of the injection cylinder 203 respectively, and the rotating plates 303 are close to the top of the injection cylinder 203.

[0031] The outer side of the chromatography column body 1 is provided with a first graduation line 4, and the outer side of the syringe 203 is provided with a second graduation line 208; multiple injection tubes 206 are arranged in a ring array, and the injection tubes 206 and the syringe 203 are integrally molded; multiple support bars 209 are fixedly connected to the outer side of the push-pull rod 205, and the support bars 209 are in contact with the inner wall of the syringe 203; the inner wall of the annular cover 202 is a smooth surface, and the side of the support bar 209 that contacts the syringe 203 is also a smooth surface; a sealing gasket is provided at the bottom of the top cover 201, and the part of the syringe 203 that passes through the top cover 201 is sealed.

[0032] In the specific implementation process, during operation, the syringe 203 can be directly extracted and removed along with the top cap 201. Then, the protein purification stock solution can be added to the interior of the chromatography column body 1. After adding the protein purification stock solution, a certain amount of dissolving solution or neutral salt solution is aspirated into the syringe 203 through multiple injection tubes 206 using the syringe 203, piston block 204, and push-pull rod 205 (during aspiration, all injection tubes 206 must be completely submerged in the container holding the dissolving solution or neutral salt solution). The syringe 203 is then inserted into the chromatography column body 1 until the bottom of the top cap 201 abuts against the top of the chromatography column body 1. The top cap 201 can then... To seal the top opening of the chromatography column body 1 with the annular cover 202, preventing impurities from entering the chromatography column body 1, the push-pull rod 205 can be pressed, which, in conjunction with the piston block 204, delivers the dissolving solution or neutral salt solution in the injection cylinder 203 into the chromatography column body 1 through the injection tube 206 with multiple injection holes 207 by squeezing pressure, until the piston block 204 is pushed to the bottom of the injection cylinder 203. This allows the dissolving solution or neutral salt solution to be injected directly into the protein purification stock solution for mixing, rather than being slowly mixed naturally. This can initially accelerate the mixing efficiency of the dissolving solution or neutral salt solution with the protein purification stock solution and initially improve the mixing uniformity.

[0033] Then, by using two rotating plates 303, the syringe 203 can be rotated, which in turn drives the rotating shaft 301 at the bottom of the syringe 203 and multiple sets of stirring rods 302 to rotate slowly, thereby further accelerating the mixing efficiency of the dissolving solution or neutral salt solution with the protein purification stock solution, and further improving the mixing uniformity. In summary, this can greatly improve the mixing efficiency and mixing uniformity of the dissolving solution or neutral salt solution with the protein purification stock solution.

[0034] The first graduation line 4 can help the staff observe the liquid level of the solution inside the chromatography column body 1, and the second graduation line 208 can help the staff observe the liquid level of the solution inside the syringe 203.

[0035] The multiple injection tubes 206 are arranged in a ring array to allow for the initial and uniform spraying of the dissolving solution or neutral salt solution into the protein purification stock solution. The injection tubes 206 and the injection cylinder 203 are integrally molded to ensure high connection strength and sealing between them, making them less prone to breakage, separation, and leakage.

[0036] Among them, the support bar 209 can effectively improve the overall stability of the push-pull rod 205, making it less prone to wobbling;

[0037] The inner wall of the annular cover 202 is smooth to reduce the friction between the annular cover 202 and the chromatography column body 1, so as to facilitate the up and down movement of the annular cover 202. Similarly, the side of the support bar 209 that contacts the syringe 203 is also smooth to reduce the friction between the support bar 209 and the inner wall of the syringe 203, so as to facilitate the up and down movement of the support bar 209 with the piston block 204 and the push rod 205.

[0038] The bottom of the top cover 201 is provided with a sealing gasket, and the part of the syringe 203 that penetrates the top cover 201 is sealed to prevent impurities from entering the chromatography column body 1.

[0039] Working principle: During operation, the syringe 203 can be directly extracted and removed along with the top cap 201. The protein purification stock solution can then be added to the interior of the chromatography column body 1. After adding the stock solution, a certain amount of dissolving solution or neutral salt solution is drawn into the syringe 203 via multiple injection tubes 206 through the syringe 203, piston block 204, and push-pull rod 205. The syringe 203 is then inserted into the chromatography column body 1 until the bottom of the top cap 201 is in contact with the top of the chromatography column body 1. The top cap 201, in conjunction with the annular cover 202, seals the top opening of the chromatography column body 1. To prevent impurities from entering the chromatography column body 1, the push-pull rod 205 is pressed, which, in conjunction with the piston block 204, delivers the solution or neutral salt solution in the injection cylinder 203 into the chromatography column body 1 through the injection tube 206 and multiple injection holes 207 by squeezing pressure, until the piston block 204 is pushed to the bottom of the injection cylinder 203. This allows the solution or neutral salt solution to be injected directly into the protein purification stock solution for mixing, rather than being slowly mixed naturally. This can initially accelerate the mixing efficiency of the solution or neutral salt solution with the protein purification stock solution and initially improve the mixing uniformity.

[0040] Then, by using two rotating plates 303, the syringe 203 can be rotated, which in turn drives the rotating shaft 301 at the bottom of the syringe 203 and multiple sets of stirring rods 302 to rotate slowly, thereby further accelerating the mixing efficiency of the dissolving solution or neutral salt solution with the protein purification stock solution, and further improving the mixing uniformity. In summary, this can greatly improve the mixing efficiency and mixing uniformity of the dissolving solution or neutral salt solution with the protein purification stock solution.

[0041] After mixing the dissolving solution or neutral salt solution with the protein purification stock solution, the piston block 204 can be kept at the bottom of the syringe 203. Then the syringe 203 can be removed. When removing the syringe 203, the piston block 204 prevents the mixed solution from entering the syringe 203, effectively avoiding the removal of residual mixed solution and thus avoiding affecting the protein purification effect. Finally, the sealing cap that matches the top opening of the chromatography column body 1 can be put on.

[0042] The preferred embodiments of this utility model disclosed above are merely illustrative of the present utility model. These preferred embodiments do not exhaustively describe all details, nor do they limit the utility model to the specific implementations described. Clearly, many modifications and variations can be made based on the content of this specification. This specification selects and specifically describes these embodiments to better explain the principles and practical applications of this utility model, thereby enabling those skilled in the art to better understand and utilize it. This utility model is limited only by the claims and their full scope and equivalents.

Claims

1. A chromatography column for protein purification, comprising a chromatography column body (1), characterized in that: The chromatography column body (1) is made of transparent material, and the top of the chromatography column body (1) is an open design; The injection mechanism (2) includes a top cover (201), an annular cover (202), an injection cylinder (203), a piston block (204), and a push-pull rod (205). The bottom of the top cover (201) is fixedly connected to the top of the annular cover (202). The bottom of the top cover (201) contacts the top opening edge of the chromatography column body (1). The size of the top cover (201) is larger than the top size of the chromatography column body (1). The inner wall of the annular cover (202) is tightly fitted to the outer wall of the chromatography column body (1). The bottom end of the injection tube (203) extends through the top cover (201) into the interior of the chromatography column body (1), and the injection tube (203) is rotatably connected to the top cover (201). The bottom of the injection tube (203) is provided with multiple injection tubes (206), and the outside of the injection tubes (206) is provided with multiple through injection holes (207). The piston block (204) is located inside the injection tube (203) and is tightly fitted to the inner wall of the injection tube (203). The bottom end of the push-pull rod (205) is fixedly connected to the top end of the piston block (204). The auxiliary stirring mechanism (3) includes a rotating shaft (301), stirring rods (302) and a rotating plate (303). The top of the rotating shaft (301) is fixedly connected to the bottom center point of the injection cylinder (203). Multiple sets of stirring rods (302) are provided on the outer circumference of the rotating shaft (301). The stirring rods (302) in the same set are located between two adjacent injection tubes (206). There are two rotating plates (303). The two rotating plates (303) are fixedly connected to the two sides of the injection cylinder (203) respectively, and the rotating plates (303) are close to the top of the injection cylinder (203).

2. The chromatography column for protein purification according to claim 1, characterized in that: The outer side of the chromatography column body (1) is provided with a first graduation line (4), and the outer side of the syringe (203) is provided with a second graduation line (208).

3. The chromatography column for protein purification according to claim 1, characterized in that: The multiple injection tubes (206) are arranged in a ring array, and the injection tubes (206) and the injection cylinder (203) are integrally molded.

4. The chromatography column for protein purification according to claim 1, characterized in that: Multiple support bars (209) are fixedly connected to the outside of the push-pull rod (205), and the support bars (209) are in contact with the inner wall of the injection cylinder (203).

5. A chromatography column for protein purification according to claim 4, characterized in that: The inner wall of the annular cover (202) is a smooth surface, and the side of the support bar (209) that contacts the injection cylinder (203) is also a smooth surface.

6. The chromatography column for protein purification according to claim 1, characterized in that: The bottom of the top cover (201) is provided with a sealing gasket layer, and the part of the syringe (203) that penetrates the top cover (201) is sealed.

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