A biological cell centrifugal separation device

Abstract

The utility model discloses a biological cell centrifugal separation device, including base, separation bucket, duck bill buckle and bucket cover, the bucket cover is connected with separation bucket through the hinge, duck bill buckle fixedly connected in the outside of separation bucket and bucket cover, the upper surface of base is fixedly connected with separation bucket, be equipped with two placing tray in the separation bucket, a plurality of connecting rods are fixedly connected between two placing tray, the upper surface of the placing tray below is equipped with a plurality of placing grooves, the upper surface of the placing tray above is equipped with the through -hole corresponding with placing groove, and the placing groove and the through -hole opposite stand in the upper and lower place and place the test tube body. The utility model not only can carry out centrifugal separation work to biological cell in the test tube of different diameters, and there is no limit to the test tube of different diameters, and simple operation, convenient to use, can avoid the test tube cover to fall off when high -speed rotation, improve the use effect of separation device, also can.

Description

Technical Field

[0001] This utility model relates to the field of centrifugation separation technology for cell detection, and in particular to a biological cell centrifugation separation device. Background Technology

[0002] Cell separation is the process of dispersing tissue materials into a cell suspension and then obtaining the target cells from it. Cell separation technology is often required in testing and analysis, and in clinical practice, cell separation requires centrifugation.

[0003] A search revealed that CN222139124U discloses a peripheral blood immune cell centrifugation device. The background section states that "before cell separation, the extracted peripheral blood needs to be stored in test tubes, and the test tubes are then installed in a cell separation device. Different test tubes have different diameters. To ensure that test tubes of different sizes can be stably placed in the cell separation device for centrifugation, a peripheral blood immune cell centrifugation device needs to be designed." To better address these issues, promote the development of industry technology, and enhance core competitiveness, this application proposes a new structural design that differs from existing technologies. Utility Model Content

[0004] The purpose of this invention is to address the shortcomings of existing technologies by proposing a biological cell centrifugation separation device.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] A biological cell centrifugation device includes a base, a separation bucket, a duckbill buckle, and a bucket lid. The bucket lid is connected to the separation bucket via a hinge. The duckbill buckle is fixedly connected to the outside of the separation bucket and the bucket lid. The separation bucket is fixedly connected to the upper surface of the base. The separation bucket contains two placement trays, and multiple connecting rods are fixedly connected between the two placement trays. The upper surface of the lower placement tray has multiple placement grooves, and the upper surface of the upper placement tray has through holes corresponding to the placement grooves. Test tube bodies are placed in the upper and lower opposing placement grooves and through holes. Test tube caps are snapped onto the tops of the test tube bodies. The upper surfaces of both placement trays have multiple dovetail grooves, and dovetail blocks are slidably connected in each of the multiple dovetail grooves. A moving block is fixedly connected to the top of the dovetail block, and an arc-shaped plate is fixedly connected to one end of the moving block. The separation bucket contains a drive assembly for moving the moving blocks.

[0007] As a further embodiment of this utility model, the drive assembly includes a threaded rod, which is rotatably connected to the upper surface of the placement disk located below via a bearing, and the threaded rod passes through the placement disk located above. The position of the threaded rod is threadedly connected to two threaded sleeves, which are respectively located above the two placement disks. Multiple wedge-shaped blocks are fixedly connected to the outer side of each of the two threaded sleeves.

[0008] As a further embodiment of this utility model, two guide rods are fixedly connected to the upper surface of the placement tray located below, and both guide rods pass through another placement tray and two threaded sleeves.

[0009] As a further embodiment of this utility model, the top ends of the two guide rods are fixedly connected to a connecting plate, and the threaded rod passes through the connecting plate. A locking nut is threadedly connected to the outer side of the threaded rod above the connecting plate.

[0010] As a further embodiment of this utility model, the upper surface of the base is provided with an installation groove, and a motor is fixedly connected inside the installation groove. One end of the motor output shaft passes through the separation barrel and is fixed to the placement plate located below.

[0011] As a further embodiment of this utility model, a rotating plate is rotatably connected inside the barrel lid via a bearing, and a rubber block that compresses the test tube lid is fixedly connected to the bottom of the rotating plate.

[0012] As a further embodiment of this utility model, the bottom of the placement tray located below is fixedly connected with a plurality of evenly distributed support rods, and the bottom ends of the plurality of support rods are fixedly connected with support wheels.

[0013] As a further embodiment of this utility model, a rubber pad is fixedly connected to one side of the arc-shaped plate, and a vent hole communicating with the mounting groove is opened at the bottom of the base.

[0014] The beneficial effects of this utility model are as follows:

[0015] 1. In this utility model, the driving mechanism pushes the moving block to move the arc-shaped plate towards the test tube, thereby allowing test tubes of different diameters to be firmly placed in the placement groove. This enables the centrifugation separation of biological cells in test tubes of different diameters, and there are no limitations on the test tubes of different diameters. Moreover, the operation is simple and convenient to use.

[0016] 2. In this utility model, by using the rotating plate and the rubber block together, after the test tube is placed, closing the tube cap will cause the rubber block to press the test tube cap tightly, and at the same time the rotating plate can rotate with the test tube, thereby preventing the test tube cap from falling off when rotating at high speed, thus improving the effectiveness of the separation device. Attached Figure Description

[0017] Figure 1 This is a three-dimensional structural diagram of a biological cell centrifugation device proposed in this utility model;

[0018] Figure 2 This is a partial cross-sectional view of a biological cell centrifugation device proposed in this utility model;

[0019] Figure 3 This is a partial exploded structural diagram of a biological cell centrifugation device proposed in this utility model;

[0020] Figure 4 This is an enlarged structural diagram of part A of a biological cell centrifugation separation device proposed in this utility model.

[0021] In the diagram: 1. Base; 2. Separation bucket; 3. Duckbill buckle; 4. Bucket lid; 5. Rotating plate; 6. Rubber block; 7. Connecting rod; 8. Mounting groove; 9. Motor; 10. Support wheel; 11. Support rod; 12. Placement tray; 13. Test tube body; 14. Test tube cap; 15. Placement groove; 16. Through hole; 17. Dovetail groove; 18. Dovetail block; 19. Threaded sleeve; 20. Threaded rod; 21. Wedge block; 22. Guide rod; 23. Moving block; 24. Arc plate; 25. Locking nut; 26. Connecting plate. Detailed Implementation

[0022] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. The described embodiments are only some embodiments of the present utility model, not all embodiments. Other embodiments obtained by those skilled in the art based on the embodiments of the present utility model without creative effort are all within the protection scope of the present utility model.

[0023] Reference Figures 1-4 A biological cell centrifugation device includes a base 1, a separation bucket 2, a duckbill buckle 3, and a bucket lid 4. The bucket lid 4 is connected to the separation bucket 2 by a hinge. The duckbill buckle 3 is fixed to the outside of the separation bucket 2 and the bucket lid 4 by bolts to fix the bucket lid 4. The separation bucket 2 is fixed to the upper surface of the base 1 by bolts. The separation bucket 2 is provided with two placement trays 12. Multiple connecting rods 7 are welded between the two placement trays 12. Multiple placement slots 15 are opened on the upper surface of the lower placement tray 12. Through holes 16 corresponding to the placement slots 15 are opened on the upper surface of the upper placement tray 12. Test tube bodies 13 are placed in the upper and lower opposing placement slots 15 and through holes 16. Test tube caps 14 are snapped onto the top of the test tube bodies 13. In use, the test tube bodies 13 containing biological cells need to be inserted into the placement slots 15 through the through holes 16.

[0024] In this invention, multiple dovetail grooves 17 are provided on the upper surface of both placement trays 12. Dovetail blocks 18 are slidably connected in each of the multiple dovetail grooves 17. A movable block 23 is welded to the top of the dovetail block 18, and an arc-shaped plate 24 is welded to one end of the movable block 23. After the test tube body 13 is placed, the arc-shaped plate 24 presses the test tube body 13, so that the test tube body 13 is stably located in the placement groove 15. This allows test tubes of different diameters to be firmly placed in the placement groove 15, thereby enabling the centrifugation of biological cells in test tubes of different diameters. There are no limitations on the test tubes of different diameters, and the operation is simple and convenient to use.

[0025] In this invention, the separating tank 2 is equipped with a driving assembly for moving the moving block 23. The driving assembly includes a threaded rod 20, which is rotatably connected to the upper surface of the lower placement plate 12 via bearings, and passes through the upper placement plate 12. Two threaded sleeves 19 are threadedly connected to the threaded rod 20, and are respectively located above the two placement plates 12. Multiple wedge-shaped blocks 21 are welded to the outer sides of each of the two threaded sleeves 19, and the inclined surfaces of the wedge-shaped blocks 21 contact the moving block 23. Two wedge-shaped blocks 21 are welded to the upper surface of the lower placement plate 12. Two guide rods 22 pass through another placement plate 12 and two threaded sleeves 19. When the arc plate 24 needs to be moved, the threaded rod 20 is rotated. The threaded rod 20 engages with the threaded sleeve 19, causing the threaded sleeve 19 to move downward along the guide rod 22. The threaded sleeve 19 will drive the wedge block 21 to move downward. During the movement, the wedge block 21 pushes the moving block 23 through its inclined surface to move along the dovetail groove 17 through the dovetail block 18. The moving block 23 will drive the arc plate 24 to move, thereby causing the arc plate 24 to squeeze the test tube body 13.

[0026] The top ends of the two guide rods 22 are welded with connecting plates 26, and the threaded rod 20 passes through the connecting plates 26. The outer side of the threaded rod 20 is threaded with a locking nut 25 above the connecting plates 26. After the test tube body 13 is fixed, the locking nut 25 is tightened to lock the threaded rod 20, thereby preventing the threaded rod 20 from rotating and causing the arc plate 24 to move.

[0027] In this invention, the upper surface of the base 1 is provided with an installation groove 8. A motor 9 is fixed inside the installation groove 8 by bolts. One end of the output shaft of the motor 9 passes through the separation bucket 2 and is fixed to the placement tray 12 located below. A rotating plate 5 is rotatably connected inside the bucket lid 4 through a bearing. A rubber block 6 that compresses the test tube lid 14 is glued to the bottom of the rotating plate 5. The bucket lid 4 is closed and fixed by a duckbill buckle 3. At this time, the rubber block 6 on the rotating plate 5 will press the test tube lid 14 tightly. Then the motor 9 is started, and the motor 9 drives the placement tray 12 to rotate, so that the biological cells in the test tube body 13 can be centrifuged. At this time, because the rubber block 6 presses the test tube lid 14 tightly, the rubber block 6 will rotate with the test tube body 13 through the rotating plate 5 under the action of friction, thereby preventing the test tube lid 14 from falling off when rotating at high speed and improving the use effect of the separation device.

[0028] The bottom of the placement tray 12 located below is fixed with multiple evenly distributed support rods 11 by bolts. The bottom ends of the multiple support rods 11 are all fixed with support wheels 10 by bolts. The support wheels 10 and support rods 11 support the placement tray 12. At the same time, when the placement tray 12 rotates, the support wheels 10 will roll, thus not increasing the friction of the placement tray 12 rotation. A rubber pad is glued to one side of the arc plate 24. The rubber pad can prevent the arc plate 24 from making hard contact with the test tube body 13. The bottom of the base 1 is provided with a vent hole that communicates with the mounting groove 8.

[0029] Working principle: In use, the test tube body 13 containing biological cells is placed into the placement groove 15 through the through hole 16. After the test tube body 13 is placed, the threaded rod 20 is rotated. The threaded rod 20 engages with the threaded sleeve 19, causing the threaded sleeve 19 to move downward along the guide rod 22. The threaded sleeve 19 drives the wedge block 21 to move downward. During the movement, the wedge block 21 pushes the moving block 23 through the dovetail block 18 along the dovetail groove 17 via its inclined surface. The moving block 23 then drives the arc plate 24 to move. This causes the arc-shaped plate 24 to press against the test tube body 13, making the test tube body 13 stably located in the placement groove 15. This allows test tubes of different diameters to be firmly placed in the placement groove 15, thus enabling the centrifugation of biological cells in test tubes of different diameters. There are no limitations on the test tubes of different diameters, and the operation is simple and convenient. After the test tube body 13 is fixed, the locking nut 25 is tightened to lock the threaded rod 20, thereby preventing the threaded rod 20 from rotating and causing the arc-shaped plate 24 to move.

[0030] Then, close the lid 4 and secure it with the duckbill buckle 3. At this time, the rubber block 6 on the rotating plate 5 will press the test tube lid 14 tightly. Then, start the motor 9, which drives the placement tray 12 to rotate, thereby enabling the biological cells in the test tube body 13 to undergo centrifugal separation. Since the rubber block 6 presses the test tube lid 14 tightly, the rubber block 6 will rotate with the test tube body 13 through the rotating plate 5 under the action of friction, thus preventing the test tube lid 14 from falling off when rotating at high speed and improving the efficiency of the separation device.

[0031] Furthermore, the terms "installation," "setup," "connection," and "socketing" should be interpreted broadly. For example, they can refer to fixed connections, detachable connections, or integral constructions; they can refer to mechanical or electrical connections; they can refer to direct connections or indirect connections via an intermediate medium, or internal connections between two devices, components, or parts. Those skilled in the art can understand the specific meaning of these terms in this application based on the specific circumstances.

Claims

1. A biological cell centrifugation device, comprising a base (1), a separation bucket (2), a duckbill buckle (3), and a bucket lid (4), wherein the bucket lid (4) is connected to the separation bucket (2) via a hinge, and the duckbill buckle (3) is fixedly connected to the outside of the separation bucket (2) and the bucket lid (4), characterized in that, The separation bucket (2) is fixedly connected to the upper surface of the base (1). The separation bucket (2) is provided with two placement trays (12). Multiple connecting rods (7) are fixedly connected between the two placement trays (12). Multiple placement slots (15) are opened on the upper surface of the placement trays (12). Through holes (16) corresponding to the placement slots (15) are opened on the upper surface of the placement trays (12). Test tube bodies (13) are placed in the placement slots (15) and through holes (16). Test tube caps (14) are snapped onto the top of the test tube bodies (13). Multiple dovetail grooves (17) are opened on the upper surface of both placement trays (12). Dovetail blocks (18) are slidably connected in the multiple dovetail grooves (17). A moving block (23) is fixedly connected to the top of the dovetail block (18). An arc-shaped plate (24) is fixedly connected to one end of the moving block (23). A driving component is provided in the separation bucket (2).

2. The biological cell centrifugation device according to claim 1, characterized in that, The drive assembly includes a threaded rod (20) which is rotatably connected to the upper surface of the lower placement disk (12) via a bearing and passes through the upper placement disk (12). The threaded rod (20) is threadedly connected to two threaded sleeves (19), which are located above the two placement disks (12). Multiple wedge blocks (21) are fixedly connected to the outer sides of each of the two threaded sleeves (19).

3. The biological cell centrifugation device according to claim 2, characterized in that, Two guide rods (22) are fixedly connected to the upper surface of the placement tray (12) located below, and both guide rods (22) pass through the other placement tray (12) and two threaded sleeves (19).

4. The biological cell centrifugation device according to claim 3, characterized in that, The top ends of the two guide rods (22) are fixedly connected to a connecting plate (26), and a threaded rod (20) passes through the connecting plate (26). A locking nut (25) is threadedly connected to the outer side of the threaded rod (20) above the connecting plate (26).

5. The biological cell centrifugation device according to claim 1, characterized in that, The upper surface of the base (1) is provided with an installation groove (8), and a motor (9) is fixedly connected inside the installation groove (8). One end of the output shaft of the motor (9) passes through the separation barrel (2) and is fixed to the placement plate (12) located below.

6. The biological cell centrifugation device according to claim 1, characterized in that, Inside the barrel lid (4), a rotating plate (5) is rotatably connected via a bearing. At the bottom of the rotating plate (5), a rubber block (6) is fixedly connected to compress the test tube lid (14).

7. The biological cell centrifugation device according to claim 1, characterized in that, The bottom of the placement tray (12) located below is fixedly connected to a plurality of evenly distributed support rods (11), and the bottom ends of the plurality of support rods (11) are fixedly connected to support wheels (10).

8. A biological cell centrifugation device according to claim 5, characterized in that, A rubber pad is fixedly connected to one side of the arc plate (24), and a vent hole connected to the mounting groove (8) is opened at the bottom of the base (1).

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