A cell concentration device

Abstract

The utility model relates to a cell concentration device, and the rotating assembly drives the rotation of the horizontally-arranged rotating support through a driving unit, the centrifugal assembly comprises a centrifugal main pipe penetrating the rotating support and first and second centrifugal branch pipes built-in, and the first and second pump bodies control the feed flow rate and the discharge flow rate respectively, the first and second centrifugal branch pipes are fixed by the rotatable centrifugal main pipe, continuous feeding and discharging are realized, the problem that the traditional intermittent centrifugation must stop fluid operation is solved, the horizontal rotating frame is adopted to cooperate with independent flow rate control, the damage of shear force to sensitive cells is effectively reduced, the double-pump cooperative control of the first and second pump bodies can realize dynamic adjustment of the concentration multiple, and the requirement of different application scenarios is met.

Description

Technical Field

[0001] This utility model relates to the field of cell separation and concentration, specifically to a cell concentration device. Background Technology

[0002] In fields such as biomedicine and cell culture, cell concentration is a crucial step. Traditional centrifugation concentration methods typically employ intermittent operation, requiring the stopping of both feed and discharge for centrifugation, resulting in low efficiency and difficulty in achieving continuous cell processing. While existing continuous flow centrifugation technology can achieve continuous operation, its equipment is complex and costly, and it may cause damage when processing certain shear-sensitive cells. Utility Model Content

[0003] In view of the above problems, this utility model provides a cell concentration device that solves the problem that existing centrifugal concentration requires stopping the feeding and discharging of materials for centrifugation.

[0004] To achieve the above objectives, this application provides a cell concentration device, including a rotating assembly, a centrifugation assembly, and a delivery assembly. The rotating assembly includes a drive unit and a rotating support. The rotating support is connected to the output end of the drive unit and is horizontally arranged. The drive unit is used to drive the rotating support to rotate horizontally. The centrifugation assembly includes a centrifuge tube body, a main centrifuge tube, a first centrifuge branch tube, and a second centrifuge branch tube. The main centrifuge tube extends from top to bottom through the rotating support and can rotate relative to the rotating support. The centrifuge tube body is located at the downward-extending end of the main centrifuge tube. The first and second centrifuge branch tubes are located inside the main centrifuge tube and protrude into the centrifuge tube body. The centrifuge tube body is used to hold the cell solution to be concentrated. The delivery assembly includes a first pump body and a second pump body. The first pump body is located on the first centrifuge branch tube, and the second pump body is located on the second centrifuge branch tube. The first pump body is used to control the input flow rate of the first centrifuge branch tube, and the second pump body is used to control the output flow rate of the second centrifuge branch tube.

[0005] In some embodiments, the centrifuge assembly further includes a fixing block disposed above the rotating bracket, the fixing block being used to engage the centrifuge main tube.

[0006] In some embodiments, a first centrifuge branch extends into the centrifuge tube by a first preset length; a second centrifuge branch extends into the centrifuge tube by a second preset length; the first preset length and the second preset length are different.

[0007] In some embodiments, the centrifuge assembly further includes a third centrifuge branch tube disposed inside the main centrifuge tube and extending into the interior of the centrifuge tube.

[0008] In some embodiments, the third centrifuge branch extends into the centrifuge tube body by a third preset length; the first preset length, the second preset length, and the third preset length are different.

[0009] In some embodiments, the delivery assembly further includes a third pump body disposed on a third centrifugal branch pipe, the third pump body being used to control the output flow rate of the third centrifugal branch pipe.

[0010] In some embodiments, the centrifuge tube is configured as a test tube, and the centrifugation assembly further includes a cover plate that covers the end of the centrifuge tube, the main centrifuge tube is airtightly connected to the cover plate, and the first centrifuge branch tube and the second centrifuge branch tube are airtightly connected to the centrifuge tube.

[0011] In some embodiments, the rotating support is configured as a curved plate or a polygonal plate.

[0012] In some embodiments, the drive unit is configured as a servo motor.

[0013] In some embodiments, the main centrifuge tube, the first centrifuge branch tube, and the second centrifuge branch tube are configured as flexible hoses.

[0014] Unlike existing technologies, in the above technical solution, the rotating component drives a horizontally arranged rotating support to rotate via a drive unit; the centrifugal component includes a main centrifugal tube running through the rotating support and built-in first and second centrifugal branch tubes; the first and second pumps control the feed flow rate and discharge flow rate, respectively. This technical solution uses a rotatable main centrifugal tube to fix the first and second centrifugal branch tubes, achieving continuous feeding and discharging, solving the problem that traditional intermittent centrifugation requires stopping fluid operation. The horizontal rotating structure combined with independent flow rate control effectively reduces shear force damage to sensitive cells. The dual-pump coordinated control of the first and second pumps enables dynamic adjustment of the concentration factor, meeting the needs of different application scenarios.

[0015] The above description of the utility model is merely an overview of the technical solution of this utility model. In order to enable those skilled in the art to better understand the technical solution of this utility model and to implement it based on the description and drawings, and to make the above-mentioned objectives and other objectives, features and advantages of this utility model easier to understand, the following description is provided in conjunction with the specific embodiments and drawings of this utility model. Attached Figure Description

[0016] The accompanying drawings are only used to illustrate the principles, implementation methods, applications, features, and effects of the present invention and other related contents, and should not be considered as limitations on the present invention.

[0017] In the accompanying drawings of the instruction manual:

[0018] Figure 1 This is a first schematic diagram of the cell concentration device described in a specific embodiment;

[0019] Figure 2 This is a second schematic diagram of the cell concentration device described in a specific embodiment;

[0020] Figure 3 This is a schematic diagram of the fixed block and the centrifugal main tube as described in a specific embodiment;

[0021] Figure 4 This is a first schematic diagram of the rotating bracket described in a specific embodiment;

[0022] Figure 5 This is a second schematic diagram of the rotating bracket described in the specific embodiment;

[0023] Figure 6 This is a schematic diagram of the centrifuge tube body, the first centrifuge branch tube, and the second centrifuge branch tube as described in a specific embodiment;

[0024] Figure 7 This is a schematic diagram of the centrifuge tube body and the first centrifuge branch tube, the second centrifuge branch tube and the third centrifuge branch tube as described in a specific embodiment.

[0025] The reference numerals used in the above figures are explained as follows:

[0026] 1. Rotating component;

[0027] 11. Drive unit;

[0028] 12. Rotate the support;

[0029] 13. Bearings;

[0030] 2. Centrifuge assembly;

[0031] 21. Centrifuge tubes;

[0032] 22. Centrifugal main pipe;

[0033] 23. First centrifugal branch pipe;

[0034] 24. Second centrifugal branch pipe;

[0035] 25. Third centrifugal branch pipe;

[0036] 26. Fixed block;

[0037] 27. Cover plate. Detailed Implementation

[0038] To illustrate in detail the possible application scenarios, technical principles, implementable specific solutions, and achievable objectives and effects of this utility model, the following description, in conjunction with the listed specific embodiments and accompanying drawings, provides a detailed explanation. The embodiments described herein are merely illustrative of the technical solutions of this utility model and are therefore intended to limit the scope of protection of this utility model.

[0039] In this document, the term "embodiment" means that a specific feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this utility model. The term "embodiment" appearing in various places throughout the specification does not necessarily refer to the same embodiment, nor does it specifically limit its independence or connection with other embodiments. In principle, in this utility model, as long as there are no technical contradictions or conflicts, the technical features mentioned in each embodiment can be combined in any way to form corresponding implementable technical solutions.

[0040] Unless otherwise defined, the technical terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains; the use of related terms herein is merely for the purpose of describing particular embodiments and is not intended to limit the invention.

[0041] In the description of this utility model, the term "and / or" is used to describe the logical relationship between objects, indicating that three relationships can exist. For example, A and / or B means: A exists, B exists, and A and B exist simultaneously. Additionally, the character " / " generally indicates that the preceding and following objects have an "or" logical relationship.

[0042] In this invention, terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any actual quantity, hierarchy, or order between these entities or operations.

[0043] Without further limitations, the use of terms such as “comprising,” “including,” “having,” or other similar expressions in this invention is intended to cover non-exclusive inclusion, which does not exclude the presence of additional elements in a process, method, or product that includes the stated elements, such that a process, method, or product that includes a series of elements may include not only those defined elements but also other elements not expressly listed, or elements inherent to such a process, method, or product.

[0044] Similar to the understanding in the Examination Guidelines, in this utility model, expressions such as "greater than," "less than," and "exceeding" are understood to exclude the stated number; expressions such as "above," "below," and "within" are understood to include the stated number. Furthermore, in the description of the embodiments of this utility model, "multiple" means two or more (including two), and similar expressions related to "multiple" are also understood in this way, such as "multiple groups" and "multiple times," unless otherwise explicitly specified.

[0045] In the description of the embodiments of this utility model, the space-related expressions used, such as "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "vertical," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," and "circumferential," indicate the orientation or positional relationship based on the orientation or positional relationship shown in the specific embodiments or drawings. They are only for the convenience of describing the specific embodiments of this utility model or for the reader's understanding, and do not indicate or imply that the device or component referred to must have a specific position, a specific orientation, or be constructed or operated in a specific orientation. Therefore, they should not be construed as limitations on the embodiments of this utility model.

[0046] Unless otherwise expressly specified or limited, the terms "installation," "connection," "linking," "fixing," and "setting," as used in the description of the embodiments of this utility model, should be interpreted broadly. For example, "connection" can be a fixed connection, a detachable connection, or an integral setting; it can be a mechanical connection, an electrical connection, or a communication connection; it can be a direct connection or an indirect connection through an intermediate medium; it can be the internal connection of two components or the interaction between two components. For those skilled in the art to which this utility model pertains, the specific meaning of the above terms in the embodiments of this utility model can be understood according to the specific circumstances.

[0047] Please see Figures 1 to 7This embodiment provides a cell concentration device, including a rotating assembly 1, a centrifugation assembly 2, and a delivery assembly. The rotating assembly 1 includes a drive unit 11 and a rotating support 12. The rotating support 12 is connected to the output end of the drive unit 11 and is horizontally arranged. The drive unit 11 drives the rotating support 12 to rotate horizontally. The centrifugation assembly 2 includes a centrifuge tube 21, a main centrifuge tube 22, a first centrifuge branch tube 23, and a second centrifuge branch tube 24. The main centrifuge tube 22 passes through the rotating support 12 from top to bottom and is rotatable relative to the rotating support 12. Centrifuge tube 21 is located at the downward-extending end of centrifuge main tube 22. First centrifuge branch tube 23 and second centrifuge branch tube 24 are placed inside centrifuge main tube 22, and the first centrifuge branch tube 23 and second centrifuge branch tube 24 protrude into the centrifuge tube 21. The centrifuge tube is used to hold the cell fluid to be concentrated. The delivery assembly includes a first pump body and a second pump body. The first pump body is located on the first centrifuge branch tube 23, and the second pump body is located on the second centrifuge branch tube 24. The first pump body is used to control the input flow rate of the first centrifuge branch tube 23, and the second pump body is used to control the output flow rate of the second centrifuge branch tube 24.

[0048] In this embodiment, the drive unit 11 refers to the power source that drives the entire rotating assembly 1. A servo motor is preferably used to ensure precise and controllable rotation speed. The rotating bracket 12 is a horizontally arranged rigid structural component used to support the centrifugal assembly 2 and transmit rotational torque. The rotating bracket 12 can be made of stainless steel or aluminum alloy to balance strength and lightweight requirements. Alternatively, non-metallic materials such as polyurethane or biological materials such as wood can be selected according to actual needs; this embodiment does not impose any limitations. At least one through hole for the centrifugal main tube 22 to pass through can be provided on the rotating bracket 12. Optionally, multiple through holes can be provided to accommodate multiple centrifugal main tubes 22, and so on. The centrifugal main tube 22 is a hollow tube that passes through the rotating bracket 12. It can rotate relative to the rotating bracket 12 via a bearing 13. It should be noted that the centrifugal main tube 22 is a flexible hose that can withstand a certain torsional torque. Furthermore, the centrifugal main tube 22 contains a first centrifugal branch tube 23 and a second centrifugal branch tube 24. Specifically, the first centrifuge branch 23 refers to the feed channel extending to the bottom of the centrifuge tube 21. The opening of the first centrifuge branch 23 should be close to the inner wall of the tube bottom to reduce dead zones for cell deposition. The second centrifuge branch 24 refers to the discharge channel located at the top of the centrifuge tube 21. The inlet end of the second centrifuge branch 24 is preferably designed with a sloping opening to enhance the collection efficiency of the supernatant. The centrifuge tube 21 is a container used to hold the cell suspension. The bottom of the centrifuge tube 21 can preferably be designed in a conical shape to promote cell precipitation and aggregation.

[0049] This embodiment also includes a conveying component, which is responsible for controlling the flow rate, flow volume, and flow direction of the first centrifuge branch pipe 23 and the second centrifuge branch pipe 24. Specifically, the conveying component includes a first pump body and a second pump body, which are fluid drive devices that control the inlet and outlet flow rates, respectively. A peristaltic pump is preferably used to avoid shear damage to the cells. In this embodiment, the conveying component may also include a control unit, which is electrically connected to the drive unit 11, the first pump body, and the second pump body to control the rotation speed of the drive unit 11 and the adjustment modes of the first and second pump bodies according to actual needs, thereby meeting diverse cell concentration and separation requirements.

[0050] In this embodiment, the drive unit 11 drives the rotating support 12 to rotate horizontally, causing the centrifuge tube 22 and centrifuge body 21 passing through it to rotate synchronously, generating a centrifugal force field. In this state, the centrifuge body 21 rotates in a nearly horizontal position. The cell suspension is continuously fed to the bottom of the centrifuge body 21 through the first centrifuge branch tube 23 under the control of the first pump. Under the action of centrifugal force, the cells settle to the bottom of the tube to form a concentrated layer, while the supernatant is discharged through the second centrifuge branch tube 24 under the control of the second pump. This embodiment achieves true continuous flow operation. Its core innovation lies in designing the centrifuge tube 22 as a dynamic cooperation system of a rotating body and a fixed branch tube, which avoids the complex sealing structure of traditional centrifuges and significantly reduces the height of the equipment through the horizontal rotating structure. The use of flexible tubing ensures the cell viability during the transport process, while the dual-pump independent control system allows for precise adjustment of the concentration factor. Compared with traditional equipment, this device maintains a high cell viability rate while achieving multiple advantages such as miniaturization, low cost, and ease of operation, making it particularly suitable for cell concentration needs in laboratory and small-scale production scenarios.

[0051] Please see Figure 3In some embodiments, the centrifuge assembly 2 further includes a fixing block 26, which is disposed above the rotating bracket 12 and is used to engage the centrifuge tube 22. The fixing block 26 is a positioning component disposed above the rotating bracket 12, and its interior has an engaging structure matching the outer diameter of the centrifuge tube 22 to limit the radial displacement of the centrifuge tube 22 while allowing it to rotate freely axially. The fixing block 26 is preferably made of a self-lubricating material such as polytetrafluoroethylene or silicone to reduce rotational friction with the centrifuge tube 22. The fixing block 26 hangs over the upper surface of the rotating bracket 12. Specifically, the fixing block 26 can be disposed in an external support structure or component, or it can be fixed to the upper surface of the rotating bracket 12 by bolts or interference fit. The central through hole of the fixing block 26 is coaxial with the through hole of the rotating bracket 12 to ensure the dynamic balance of the centrifuge tube 22 during high-speed rotation. The upper end face of the fixing block 26 can be designed as a flange structure to share part of the radial load of the centrifuge tube 22. In this embodiment, by setting a fixing block 26, the rotational freedom of the centrifuge tube 22 is guaranteed, effectively suppressing tube vibration caused by centrifugal force, making the entire concentration process run more smoothly and reliably.

[0052] Please see Figure 6 In some embodiments, the first centrifugal branch tube 23 protrudes into the centrifugal tube body 21 by a first preset length; the second centrifugal branch tube 24 protrudes into the centrifugal tube body 21 by a second preset length; the first preset length and the second preset length are different.

[0053] In this embodiment, the first preset length of the first centrifugal branch pipe 23 extending into the centrifugal tube body 21 can be understood as a specific distance between the end of the first centrifugal branch pipe 23 and the bottom of the centrifugal tube body 21. The first preset length is preferably 80%-90% of the total height of the centrifugal tube body 21, ensuring that the feed inlet at the end of the first centrifugal branch pipe 23 is close to the sedimentation zone but does not contact the sediment at the bottom of the tube.

[0054] The second preset length of the second centrifugal branch tube 24 extending into the centrifugal tube body 21 can be understood as a specific distance between the end of the second centrifugal branch tube 24 and the top of the tube body. The second preset length is preferably 10%-20% of the total height of the centrifugal tube body 21, ensuring that the outlet of the second centrifugal branch tube 24 is always located in the supernatant layer. The difference between the first and second preset lengths creates spatial separation between the feed and discharge zones. This asymmetrical layout allows fresh cell fluid to be concentrated to fully participate in the centrifugation process after entering from the bottom, while the clarified liquid is stably discharged from the top. Optionally, the length difference between the first centrifugal branch tube 23 and the second centrifugal branch tube 24 should be at least 30% greater than the height of the centrifugal tube body 21. This geometric parameter, along with the rotational speed and flow rate, constitutes a key control dimension for separation efficiency. In particular, the end of the first centrifugal branch tube 23 can be set as a 45° oblique cut to reduce feed turbulence, and the inlet end of the second centrifugal branch tube 24 is preferably designed as a funnel shape to expand the supernatant collection range.

[0055] This embodiment optimizes the spatial separation between the feeding and discharging zones by setting the first centrifuge branch 23 and the second centrifuge branch 24 to different protrusion lengths. The end of the first centrifuge branch 23 is close to the sedimentation zone but avoids disturbing the sediment at the bottom of the tube, ensuring that the cell fluid to be concentrated enters efficiently from the bottom and participates in centrifugation. The end of the second centrifuge branch 24 is located in the supernatant layer, allowing the clarified liquid to be discharged stably, which significantly improves the separation efficiency.

[0056] Please see Figure 7 In some embodiments, the centrifuge assembly 2 further includes a third centrifuge branch pipe 25, which is disposed inside the centrifuge main pipe 22 and extends into the centrifuge tube body 21.

[0057] The third centrifuge branch 25 refers to an auxiliary fluid channel located within the main centrifuge tube 22. The extended end of the third centrifuge branch 25 protrudes into the centrifuge tube body 21 by a third preset length. The protruding length of the third centrifuge branch 25 is preferably located in the intermediate layer region between the first centrifuge branch 23 and the second centrifuge branch 24. The opening position of the end of the third centrifuge branch 25 can be adjusted to 40%-60% of the height of the centrifuge tube body 21 according to specific application requirements. The third centrifuge branch 25 can achieve multiple functional expansions: as a secondary feed channel, it can be used to add buffer solution or reagents; as a monitoring channel, it can be connected to a sensor to detect the separation interface in real time; as an auxiliary discharge channel, it can selectively discharge intermediate components. The introduction of the third centrifuge branch 25 enables the device to have multi-stage separation or online parameter control capabilities, significantly improving process flexibility.

[0058] In some embodiments, the third centrifugal branch 25 extends into the centrifugal tube 21 by a third preset length; the first preset length, the second preset length, and the third preset length are different.

[0059] The third centrifugal branch pipe 25 serves as an auxiliary fluid channel within the main centrifugal pipe 22. The extended end of the third centrifugal branch pipe 25 protrudes into the centrifugal tube body 21 at a specific third preset length, which differs from the first preset length of the first centrifugal branch pipe 23 and the second preset length of the second centrifugal branch pipe 24. Optionally, the third preset length can be adjusted to be flush with the first preset length, or adjusted to be flush with the second preset length, depending on actual needs.

[0060] Preferably, the end opening of the third centrifugal branch 25 is located in the intermediate layer region between the first centrifugal branch 23 and the second centrifugal branch 24, forming a three-level gradient fluid channel system. The differentiated length design allows the third centrifugal branch 25 to independently perform its functional characteristics, thereby enabling switching between different operating modes. Through this multi-length gradient branch configuration, the device achieves more precise separation control and more flexible process adaptability.

[0061] In some embodiments, the delivery assembly further includes a third pump body disposed on the third centrifugal branch pipe 25, the third pump body being used to control the output flow rate of the third centrifugal branch pipe 25.

[0062] In this embodiment, the third pump body serves as an independent fluid drive unit 11, forming a multi-stage flow rate control system together with the first and second pump bodies. The third pump body allows the third centrifuge branch 25 to be independently adjusted according to actual process requirements, determining its buffer replenishment rate when used as a secondary feed channel, its sampling frequency when used as a monitoring channel, or its intermediate component discharge rate when used as an auxiliary discharge channel. Through precise control of the third pump body, operators can dynamically adjust the intermediate layer separation process, optimizing the overall separation effect without affecting bottom sedimentation and top clarification. This further enhances the device's adaptability to complex separation processes, particularly for biological sample separation processes requiring precise control of intermediate component processing.

[0063] Please see Figure 6 and Figure 7 In some embodiments, the centrifuge tube 21 is configured as a test tube, and the centrifuge assembly 2 further includes a cover plate 27, which covers the end of the centrifuge tube 21. The main centrifuge tube 22 is airtightly connected to the cover plate 27, and the first centrifuge branch tube 23 and the second centrifuge branch tube 24 are airtightly connected to the centrifuge tube 21.

[0064] In this embodiment, the cover plate 27 can be a rubber stopper, a plastic cover plate 27, or other components that ensure an airtight seal on the test tube. The cover plate 27 forms an airtight seal with the open end of the centrifuge tube 21, while the main centrifuge tube 22 passes through the cover plate 27 and maintains an airtight connection with it. The first centrifuge branch tube 23 and the second centrifuge branch tube 24 are both airtightly inserted into the centrifuge tube 21, forming a sealed fluid channel. The airtight connection design shown in this embodiment ensures the independent controllability of the first centrifuge branch tube 23, the second centrifuge branch tube 24, and the third centrifuge branch tube 25 (if any) during centrifugation, preventing cross-contamination of fluids or pressure leakage. The cover plate 27 effectively seals the operating space of the centrifuge tube 21, creating a stable sealed environment for the centrifugation process, ensuring the stable operation of the entire cell concentration device under positive or negative pressure conditions, and meeting the special pressure environment requirements of different separation processes.

[0065] Please see Figure 4 and Figure 5 In some embodiments, the rotating support 12 is configured as a curved plate or a polygonal plate. Specifically, the curved plate may be a disc, ring, sector plate, or arc plate, and the polygonal plate may be a triangular plate, rectangular plate, trapezoidal plate, etc.

[0066] Please see Figure 1 and Figure 2 In some embodiments, the drive unit 11 is configured as a servo motor. Optionally, the servo motor can be a bidirectional servo motor to achieve centrifugation operations in two directions, meeting different experimental requirements.

[0067] In some embodiments, the main centrifuge tube 22, the first centrifuge branch tube 23, and the second centrifuge branch tube 24 are configured as flexible tubing. The flexible tubing shown in this embodiment is made of a material that possesses good torque transmission, biocompatibility, and chemical stability. For example, it can be medical-grade silicone tubing (with excellent flexibility, high-temperature sterilization resistance, and physiological inertness), PTFE (polytetrafluoroethylene) tubing with reinforcing fibers (balancing chemical resistance and moderate rigidity), medical-grade PVC (polyvinyl chloride) tubing (economical and practical, and compliant with USP Class VI standards), and block copolymers such as C-Flex (combining the flexibility of silicone with higher kink resistance). This ensures the integrity of the tubing structure under centrifugal force while meeting aseptic operation requirements. Preferably, silicone and PTFE materials are particularly suitable for experimental scenarios requiring repeated high-temperature sterilization or contact with organic solvents, while fiber-reinforced tubing provides better resistance to torsional deformation, ensuring the stability of fluid delivery during centrifugation. The material of the flexible tubing can be selected according to actual needs.

[0068] Unlike existing technologies, in the above technical solution, the rotating component 1 drives the horizontally arranged rotating support 12 to rotate via the drive unit 11; the centrifugal component 2 includes a centrifugal main tube 22 that passes through the rotating support 12 and built-in first centrifugal branch tubes 23 and second centrifugal branch tubes 24; the first pump body and the second pump body control the feed flow rate and the discharge flow rate, respectively. This technical solution uses the rotatable centrifugal main tube 22 to fix the first centrifugal branch tubes 23 and the second centrifugal branch tubes 24, realizing continuous feeding and discharging, solving the problem that traditional intermittent centrifugation must stop fluid operation. The horizontal rotating structure combined with independent flow rate control effectively reduces the damage of shear force to sensitive cells. With the dual-pump coordinated control of the first pump body and the second pump body, the concentration ratio can be dynamically adjusted to meet the needs of different application scenarios.

[0069] Finally, it should be noted that although the above embodiments have been described in the text and drawings of this utility model, this should not limit the scope of patent protection of this utility model. Any technical solutions resulting from equivalent structural or procedural substitutions or modifications made based on the essential concept of this utility model and utilizing the content described in the text and drawings of this utility model, as well as the direct or indirect application of the technical solutions of the above embodiments to other related technical fields, are all included within the scope of patent protection of this utility model.

Claims

1. A cell concentration device, characterized in that, include: A rotating assembly includes a drive unit and a rotating bracket, the rotating bracket being connected to the output end of the drive unit, the rotating bracket being arranged horizontally, and the drive unit being used to drive the rotating bracket to rotate horizontally; A centrifuge assembly includes a centrifuge tube body, a centrifuge main tube, a first centrifuge branch tube, and a second centrifuge branch tube. The centrifuge main tube extends from top to bottom through the rotating support and is rotatable relative to the rotating support. The centrifuge tube body is located at the downward-extending end of the centrifuge main tube. The first centrifuge branch tube and the second centrifuge branch tube are placed inside the centrifuge main tube and protrude into the interior of the centrifuge tube body. The centrifuge tube body is used to hold the cell solution to be concentrated. The conveying assembly includes a first pump body and a second pump body. The first pump body is disposed on the first centrifugal branch pipe, and the second pump body is disposed on the second centrifugal branch pipe. The first pump body is used to control the input flow rate of the first centrifugal branch pipe, and the second pump body is used to control the output flow rate of the second centrifugal branch pipe.

2. The cell concentration apparatus according to claim 1, characterized in that, The centrifugation assembly also includes: A fixing block is disposed above the rotating bracket, and the fixing block is used to engage the centrifuge tube.

3. The cell concentration apparatus according to claim 1, characterized in that, The first centrifuge branch tube extends into the centrifuge tube body by a first preset length; The second centrifuge branch extends into the centrifuge tube body by a second predetermined length; The first preset length and the second preset length are different.

4. The cell concentration apparatus according to claim 3, characterized in that, The centrifugation assembly also includes: The third centrifugal branch pipe is disposed inside the main centrifugal tube and extends into the interior of the centrifugal tube.

5. The cell concentration apparatus according to claim 4, characterized in that, The third centrifuge branch tube extends into the centrifuge tube body by a third predetermined length; The first preset length, the second preset length, and the third preset length are different.

6. The cell concentration apparatus according to claim 4, characterized in that, The conveying assembly also includes: A third pump body is installed on the third centrifugal branch pipe, and the third pump body is used to control the output flow rate of the third centrifugal branch pipe.

7. The cell concentration apparatus according to claim 1, characterized in that, The centrifuge tube is configured as a test tube, and the centrifugation assembly further includes: A cover plate is fitted over the end of the centrifuge tube, the main centrifuge tube is airtightly connected to the cover plate, and the first centrifuge branch tube and the second centrifuge branch tube are airtightly connected to the centrifuge tube.

8. The cell concentration apparatus according to claim 1, characterized in that, The rotating support is configured as a curved plate or a polygonal plate.

9. The cell concentration apparatus according to any one of claims 1 to 8, characterized in that, The drive unit is configured as a servo motor.

10. The cell concentration apparatus according to any one of claims 1 to 8, characterized in that, The main centrifuge tube, the first centrifuge branch tube, and the second centrifuge branch tube are configured as flexible hoses.

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