Electrical stimulation cell culture device

Abstract

The utility model relates to an electric stimulation cell culture device which comprises, from top to bottom, a cover, a fence, a conductive glass plate and a fixing frame. The fence is open at the top and bottom and closed around. A pressing mechanism is arranged between the fence and the cover or the fixing frame. The pressing mechanism is used to fix the fence on the fixing frame. The conductive glass plate is arranged between the fence and the fixing frame. The bottom of the fence is in sealing contact with the conductive glass plate to form a cell culture chamber. The length of the conductive glass plate is longer than that of the fence at both ends to form an electrode connecting part for connecting with an electrode. The electric stimulation cell culture device provided by the utility model avoids direct contact between the electrode and the culture medium, effectively avoids the risk of cell contamination, and has a simple overall structure, which can simplify experimental operation and ensure normal cell culture environment while applying electric stimulation.

Description

Technical Field

[0001] This utility model relates to a cell culture device for human or animal cells, and more particularly to an electrically stimulated cell culture device. Background Technology

[0002] Electrical stimulation (ES), as an important intervention in modern clinical electrophysiology, directly acts on damaged muscle and nerve tissue by applying controlled current or electric field, or promotes functional recovery through neural reflex modulation. In recent years, ES has been increasingly widely used in tissue engineering and regenerative medicine. Its mechanism involves activating key intracellular signaling pathways, thereby regulating cell proliferation, directed differentiation of stem cells, and tissue regeneration.

[0003] Existing cell electrostimulation devices, such as the cell culture device and its electrostimulation component disclosed in patent application CN119193321A, use electrodes to directly contact the cell culture medium. Although this method is simple and easy to operate, the metal components in the electrodes can dissolve in the liquid culture medium, leading to changes in the solution pH and even cell contamination.

[0004] Furthermore, while using a salt bridge as an intermediate medium to transfer charge can avoid direct contact between the electrodes and the cell culture medium, the interface between the salt bridge and the culture chamber is prone to leakage, increasing the risk of contamination (such as microbial invasion), and the device connection is relatively cumbersome. Although using electromagnetic fields to indirectly and non-invasively stimulate cells can achieve a wide variety of waveforms, the required hardware is complex, requiring dedicated coils or magnetic field generators, which are expensive, and cannot guarantee normal cell culture conditions while stimulating cells. Utility Model Content

[0005] The technical problem to be solved by this invention is to provide a cell electrical stimulation device that can effectively avoid the risk of cell contamination and has a simplified structure.

[0006] To solve the above problems, the technical solution adopted by this utility model is as follows: A cell culture device with electrical stimulation, the key technology of which is that it includes a cover, a fence, a conductive glass plate and a fixing frame from top to bottom. The fence is open at the top and bottom and closed on all sides. The cover is placed on top of the fence. A clamping mechanism is provided between the fixing frame and the fence or the cover. The clamping mechanism is used to fix the fence to the fixing frame. The conductive glass plate is placed between the fence and the fixing frame. The bottom of the fence is in sealed contact with the conductive glass plate to form a cell culture chamber. Both ends of the conductive glass plate in the length direction are longer than the fence to form electrode connection parts for connecting with electrodes.

[0007] In one embodiment of this utility model, the fence is rectangular, and the clamping mechanism includes protrusions fixedly disposed on the outer sides of the two side walls in the width direction of the fence and two buckles disposed on the fixing frame, the buckles being adapted to the protrusions.

[0008] As one embodiment of this utility model, the fixing frame includes a base plate, with baffles on three sides and an open side.

[0009] As one embodiment of this utility model, the bottom of the fixing frame is provided with a hollow observation window.

[0010] In one embodiment of this utility model, a partition is provided in the middle of the fence, which divides the fence into two culture chambers.

[0011] In one embodiment of this utility model, a sealing ring is provided at the bottom of the fence, and the fence is in sealed contact with the conductive glass plate through the sealing ring.

[0012] As one embodiment of this utility model, it also includes two electrode patches, one side of which is attached to the conductive glass plate and the other side is used to connect to an electrode.

[0013] In one embodiment of this utility model, the electrode patch includes a backing layer and a conductive gel attached to the backing layer.

[0014] In one embodiment of this utility model, the cover and the fence are made of transparent plastic material that facilitates observation of cell morphology.

[0015] In one embodiment of this utility model, the top sidewall of the fence is provided with a protrusion, and the cover is provided with a groove that matches the protrusion.

[0016] The beneficial effects of adopting the above technical solution are as follows: The electrostimulation cell culture device provided by this utility model has a cell culture chamber formed by the bottom of the enclosure being sealed in contact with a conductive glass plate. The two ends of the conductive glass plate along its length are located outside the enclosure, forming electrode connection parts. The electrodes are connected to the electrode connection parts of the conductive glass plate, avoiding direct contact with the culture medium and effectively avoiding the risk of cell contamination. Furthermore, the overall structure of this device is simple, which can simplify experimental operations and ensure a normal cell culture environment while applying electrical stimulation. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the overall structure of the present invention after assembly.

[0018] Figure 2 This is an exploded structural diagram of the present invention.

[0019] Figure 3 This is a top-down structural diagram of the fence.

[0020] Figure 4 This is a diagram showing a sealing ring installed at the bottom of the fence.

[0021] Figure 5 This is a structural diagram of the fixed frame.

[0022] The components include: 1. Cover, 2. Fence, 2-1. Protruding rib, 2-2. Partition, 2-3. Protrusion, 2-4. Reinforcing rib, 2-5. Sealing ring, 3. Fixing frame, 3-1. Buckle, 3-2. Baffle, 3-3. Base plate, 4. Conductive glass plate, 5. Electrode patch. Detailed Implementation

[0023] To make the objectives, technical solutions and advantages of this utility model clearer, the utility model will be clearly and completely described below in conjunction with specific embodiments.

[0024] like Figure 1 and Figure 2 The illustrated electrostimulated cell culture device comprises, from top to bottom, a cover 1, a fence 2, a conductive glass plate 4, and a fixing frame 3. The fence 2 is open at the top and bottom and closed on all sides. The cover 1 is placed on top of the fence 2 to isolate dust and bacteria, but there is a ventilation channel between the cover 1 and the fence 2. The top sidewall of the fence 2 is provided with protrusions 2-3, and the cover 1 is provided with grooves that fit the protrusions 2-3. When the cover 1 is placed on the fence 2, the protrusions 2-3 are embedded in the grooves of the cover 1, preventing the cover 1 from sliding. Multiple vertical reinforcing ribs 2-4 are provided on the inner or outer wall of the fence 2 to increase the structural strength of the fence and reduce deformation.

[0025] A clamping mechanism is provided between the fixing frame 3 and the fence 2 or the cover 1. The clamping mechanism is used to fix the fence 2 on the fixing frame 3. The conductive glass plate 4 is pressed between the fence 2 and the fixing frame 3. The bottom of the fence 2 is in sealed contact with the conductive glass plate 4 to form a cell culture chamber and prevent cell culture medium from leaking out.

[0026] Both ends of the conductive glass plate 4 are longer than the fence 2, forming an electrode connection part for connection with the electrode; specifically, in this embodiment, the conductive glass plate 4 is a double-sided ITO coated conductive glass with a specification of 70mm*25mm*1.1mm.

[0027] The cover 1 and the fence 2 are made of transparent plastic material that facilitates observation of cell morphology, specifically polyethylene material; the fixing frame 3 can be made of semi-transparent polypropylene material.

[0028] like Figure 2 and Figure 5As shown, in this embodiment, the fixing frame 3 includes a base plate 3-3, with baffles 3-2 on three sides of the base plate 3-3 and an open side for placing the conductive glass plate 4; the bottom of the fixing frame 3 is provided with a hollow observation window for easy observation with a light microscope during cell culture (when the fixing frame 3 is not disassembled).

[0029] like Figure 2 , 3 As shown in Figure 5, the fence 2 is rectangular. The clamping mechanism includes protrusions 2-1 fixedly disposed on the outer sides of the two side walls of the fence 2 in the width direction and two latches 3-1 disposed on the fixing frame 3. The latches 3-1 are adapted to the protrusions 2-1. The bottom of the latches 3-1 is connected to the two sides of the base plate 3-3 in the width direction through a thin sheet. The latches 3-1 can swing slightly on the thin sheet. The protrusions 2-1 are arranged horizontally to facilitate the adjustment of the position of the fence 2 on the fixing frame 3.

[0030] As a feasible implementation, the clamping mechanism can also be set between the cover 1 and the fixing frame 3. Specifically, a protrusion adapted to the buckle 3-1 can be provided on the side wall of the cover 1. The buckle clamps the fence 2 and the conductive glass plate 4 while clamping the cover 1.

[0031] See Figure 4 As shown, a sealing ring 2-5 is provided at the bottom of the fence 2, and the fence 2 is in sealed contact with the conductive glass plate 4 through the sealing ring 2-5; when the buckle 3-1 fastens the protrusion 2-1, the fence 2 is subjected to downward pressure, which makes the sealing ring 2-5 and the conductive glass plate 4 come into tight contact to form a seal.

[0032] As a preferred technical solution, a partition 2-2 is provided in the middle of the enclosure 2, dividing the enclosure 2 into two culture chambers. The partition 2-2 can be fixedly connected to the inner wall of the enclosure 2, or it can be designed as a detachable connection. In the detachable connection case, a vertical slot is provided on the inner wall of the enclosure 2 for inserting the partition 2-2. During cell culture, the two culture chambers can use different culture media or different cell types for culture.

[0033] In this embodiment, the two culture chambers are of equal size, with a bottom area of ​​4.55 cm². 2 This size facilitates subsequent image acquisition under a confocal fluorescence microscope.

[0034] The device described in this embodiment also includes two electrode patches 5. One side of each electrode patch 5 is attached to the conductive glass plate 4, and the other side is used to connect to an electrode. Each electrode patch 5 includes a backing layer and a conductive gel attached to the backing layer. The electrodes of the electrostimulator are electrically connected to the electrode patches 5, thereby achieving an electrical connection with the conductive glass plate 4.

[0035] Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. An electrically stimulated cell culture device, characterized in that, It comprises, from top to bottom, a cover (1), a fence (2), a conductive glass plate (4), and a fixing frame (3). The fence (2) is open at the top and bottom and closed on all sides. The cover (1) covers the top of the fence (2). A clamping mechanism is provided between the fixing frame (3) and the fence (2) or the cover (1). The clamping mechanism is used to fix the fence (2) on the fixing frame (3). The conductive glass plate (4) is pressed between the fence (2) and the fixing frame (3). The bottom of the fence (2) is in sealed contact with the conductive glass plate (4) to form a cell culture chamber. Both ends of the conductive glass plate (4) in the length direction are longer than the fence (2) to form an electrode connection part for connecting with the electrode.

2. The electrostimulated cell culture device according to claim 1, characterized in that, The fence (2) is rectangular. The clamping mechanism includes protrusions (2-1) fixedly disposed on the outer sides of the two side walls in the width direction of the fence (2) and two buckles (3-1) disposed on the fixing frame (3). The buckles (3-1) are adapted to the protrusions (2-1).

3. The electrostimulated cell culture device according to claim 1, characterized in that, The fixing frame (3) includes a base plate (3-3), with baffles (3-2) on three sides and an open side on the other side.

4. The electrostimulated cell culture device according to claim 1, characterized in that, The bottom of the mounting bracket (3) is provided with a hollow observation window.

5. The electrostimulated cell culture device according to claim 1, characterized in that, The fence (2) has a partition (2-2) in the middle, which divides the fence (2) into two culture chambers.

6. The electrostimulated cell culture device according to claim 1, characterized in that, The bottom of the fence (2) is provided with a sealing ring (2-5), and the fence (2) is in sealed contact with the conductive glass plate (4) through the sealing ring (2-5).

7. The electrostimulated cell culture device according to claim 1, characterized in that, It also includes two electrode patches (5), one side of which is attached to the conductive glass plate (4), and the other side is used to connect to an electrode.

8. The electrostimulated cell culture device according to claim 7, characterized in that, The electrode patch (5) includes a backing layer and a conductive gel attached to the backing layer.

9. The electrostimulated cell culture device according to claim 1, characterized in that, The cover (1) and fence (2) are made of transparent plastic material that facilitates observation of cell morphology.

10. The electrostimulated cell culture device according to claim 1, characterized in that, The top sidewall of the fence (2) is provided with protrusions (2-3), and the cover (1) is provided with grooves that are adapted to the protrusions (2-3).

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