A portable disposable stem cell monoclonal transfer device

Abstract

The utility model discloses a portable disposable stem cell monoclonal transfer device. Portable disposable stem cell monoclonal transfer device, include: the outer sheath pipe, the front end of outer sheath pipe is provided with the clamping mechanism, the tail end of outer sheath pipe is provided with the pressing plate, the clamping mechanism includes a plurality of with the flexible connection of outer sheath pipe blade, the blade can overturn to the outside, install push -pull rod in the outer sheath pipe, one end of push -pull rod with pressing plate fixed connection, the other end of push -pull rod with the middle part of blade is connected through the connecting line. The portable disposable stem cell monoclonal transfer device provided by the utility model through setting adopts the outer sheath pipe of disposable medical material manufacture, pressing plate and including push -pull rod, a plurality of blades, the clamping mechanism of connecting line, can under normal pressure, the single stem cell clone is conveniently picked, has the advantages such as small volume, not easy cross -contamination, not easy damage cell.

Description

Technical Field

[0001] This utility model relates to the field of experimental instrument technology, and in particular to a portable, disposable stem cell monoclonal transfer device. Background Technology

[0002] Transfer instruments are required when transferring clonal stem cells. Common transfer instruments include: picking needles made of glass capillary tubes or pasteur pipettes, pipettes with sterile tips, cell scrapers or cloning loops.

[0003] However, the picking needles made by pulling glass capillaries or Pasteur pipettes are prone to cross-contamination when reused; the open tip of the pipette is difficult to completely peel off adherent clones, and negative pressure operation can easily damage cells and aspirate impurities; the cloning loop is too large to accurately locate single clones, and scraping between cells can easily damage cells.

[0004] Therefore, it is necessary to provide a portable, disposable stem cell monoclonal transfer device to solve the above-mentioned technical problems. Utility Model Content

[0005] In view of the above situation and to overcome the defects of the existing technology, this utility model provides a portable disposable stem cell monoclonal transfer device that has the advantages of small size, low risk of cross-contamination, and low risk of damage to stem cell monoclonal cells.

[0006] To achieve the above objectives, the technical solution adopted by this utility model is as follows:

[0007] A portable, disposable stem cell monoclonal picking stick includes: an outer sheath, a clamping mechanism at the front end of the outer sheath, and a pressing plate at the rear end of the outer sheath; the clamping mechanism includes multiple blades flexibly connected to the outer sheath, the blades being able to flip outwards, and a push-pull rod installed inside the outer sheath, one end of which is fixed to the pressing plate, and the other end of which is connected to the middle of the blades via a connecting line.

[0008] Preferably, the outer sheath tube is 10-15 cm long and 3-5 mm in outer diameter.

[0009] Preferably, the number of leaves is five.

[0010] Preferably, an elastic component is provided inside the outer sheath tube, and the elastic component is located between the outer sheath tube and the push-pull rod, which enables the push-pull rod to automatically reset.

[0011] Preferably, the elastic component includes a retaining ring fixedly installed inside the outer sheath tube, a push-pull rod passing through the retaining ring, an integrally formed limiting ring on the push-pull rod, and a mounting spring sleeved on the push-pull rod.

[0012] Preferably, multiple blades are stacked sequentially.

[0013] Preferably, the distal end of the blade is folded inward to form a hook-shaped or spoon-shaped structure.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] (1) This utility model, by setting an outer sheath tube made of disposable medical materials, a pressing plate, and a clamping mechanism including a push-pull rod, multiple blades, and connecting wires, can easily pick up a single stem cell clone under normal pressure. It has the advantages of small size, easy cross-contamination, and easy cell damage.

[0016] (2) By setting an elastic component inside the outer sheath tube, this utility model can facilitate the automatic reset of the push-pull rod, making it convenient for one-handed operation of the device;

[0017] (3) By setting the far end of the blade to fold inward to form a hook-shaped or spoon-shaped structure, this utility model can easily push the stem cell monoclonal tube to move towards the center of the outer sheath. Attached Figure Description

[0018] Figure 1 A schematic diagram of the structure of the portable disposable stem cell monoclonal transfer device provided by this utility model;

[0019] Figure 2 for Figure 1 A schematic diagram of the gripping mechanism in the portable, disposable stem cell monoclonal transfer device shown.

[0020] Figure 3 for Figure 1 The diagram shows the use of the portable, single-use stem cell monoclonal transfer device.

[0021] Figure 4 for Figure 1 A magnified structural diagram of part A in the middle;

[0022] Figure 5 for Figure 1 A schematic diagram of the closed clamping mechanism in the portable disposable stem cell monoclonal transfer device shown;

[0023] Figure 6 for Figure 1 A side view of the blades in the portable, disposable stem cell monoclonal transfer device shown.

[0024] The corresponding names of the attached figures are: 1-outer sheath, 2-clamping mechanism, 3-pressing plate, 4-push-pull rod, 5-blade, 6-connecting line, 7-fixing ring, 8-limiting ring, 9-spring. Detailed Implementation

[0025] The present invention will be further described below with reference to the accompanying drawings and embodiments. The embodiments of the present invention include, but are not limited to, the following embodiments.

[0026] Example 1:

[0027] like Figure 1-6 As shown, this utility model provides a portable disposable stem cell monoclonal transfer device, comprising: an outer sheath 1: slender, hollow, tubular structure. The material is disposable medical plastic (such as polypropylene PP, polystyrene PS, or medical-grade ABS) to ensure sterility and biocompatibility. The length is convenient for hand operation (e.g., 10-15cm), and the diameter is moderate (e.g., outer diameter 3-5mm). A clamping mechanism 2 is provided at the front end of the outer sheath 1, and a pressing plate 3 is provided at the rear end. The clamping mechanism 2 includes multiple blades 5 flexibly connected to the outer sheath 1 (e.g., using flexible medical silicone strips to connect and fix the outer sheath 1 and the blades 5). The blades 5 are triangular or trapezoidal, and the multiple blades 5 are arranged in a circular array around the center of the outer sheath 1. The blades 5 can be flipped outwards, with a maximum flip angle of 120 degrees. In this embodiment, the number of blades 5 is five. A push-pull rod 4 is installed inside the outer sheath 1. One end of the push-pull rod 4 is fixed to the pressing plate 3, and the other end is connected to the middle of the blades 5 through a certain... Connect the strong connecting line 6, hold the outer sheath tube 1, and press the end pressing plate 3 of the push-pull rod 4 to lower the push-pull rod 4. Through the connecting line 6, the blades 5 flip, opening the bottom channel of the outer sheath tube 1. Align the bottom of the outer sheath tube 1 with the single cloning cell and lower it, surrounding the single cloning cell (cloning stem cell) in the center of the multiple blades 5. Then, pull the pressing plate 3 upwards, causing the blades 5 to flip towards the center of the outer sheath tube 1. With appropriate raising and lowering of the outer sheath tube 1, the five blades 5 tighten and close, sealing the bottom of the outer sheath tube 1. The single cloning cell is then scooped from the storage container and placed inside the outer sheath tube 1. The process of placing cloning cells is the same as above. It is worth noting that, unless otherwise specified, all materials in this embodiment are disposable medical plastics, and the operation of the instrument requires the use of appropriate microscopes or other equipment. The instrument is for single use only, which reduces the risk of cross-contamination. It operates under normal pressure, which minimizes cell damage. Its small size makes it easy to handle and allows for precise positioning of single clones. Only one cell is extracted at a time, minimizing cell scraping damage. This enables complete encapsulation and removal of adherent clones (without scraping or aspiration).

[0028] By setting up an outer sheath tube 1 made of disposable medical materials, a pressing plate 3, and a clamping mechanism 2 including a push-pull rod 4, multiple blades 5, and connecting lines 6, a single stem cell clone can be easily picked up under normal pressure. It has the advantages of small size, low risk of cross-contamination, and low risk of cell damage.

[0029] Example 2:

[0030] like Figure 1 and Figure 4 As shown, in this embodiment, an elastic component is provided inside the outer sheath 1. The elastic component is located between the outer sheath 1 and the push-pull rod 4 and is used for the automatic spring-back reset of the push-pull rod 4. The elastic component can be a medical rubber strip or other structure. In this embodiment, the elastic component includes a fixing ring 7 fixedly installed inside the outer sheath 1. The push-pull rod 4 passes through the fixing ring 7. An integrally formed limiting ring 8 is provided on the push-pull rod 4. A spring 9 is sleeved on the push-pull rod 4. The fixing ring 7, the limiting ring 8, and the spring 9 are all made of medical plastic. In use, the outer sheath 1 is held with one hand and the thumb presses the push-pull rod 4, causing the blade 5 to flip open, opening the bottom end of the outer sheath 1. The spring 9 is compressed. After releasing the pressure on the tail end of the push-pull rod 4, the spring 9 causes the push-pull rod 4 to reset and drive the blade 5 to rotate in the opposite direction, closing the bottom end of the outer sheath 1. Therefore, the device can be easily operated with one hand.

[0031] By setting an elastic component inside the outer sheath tube 1, the push-pull rod 4 can be automatically reset, facilitating one-handed operation of the device.

[0032] Example 3:

[0033] like Figure 5 As shown, in this embodiment, the multiple blades 5 of the clamping mechanism 2 are stacked sequentially, that is, the left side of the blade 5 presses down on the blade 5 to its left, and the right side is pressed down by the blade 5 to its right. This cycle continues, and after the connecting line 6 is tightened, the multiple blades 5 are stacked layer by layer to seal the bottom end of the outer sheath tube 1.

[0034] By setting up a specially designed clamping mechanism 2, the bottom end of the outer sheath tube 1 can be easily closed.

[0035] Example 4:

[0036] like Figure 6 As shown, the distal end of leaf 5 (the end away from its connection with the outer sheath 1) folds inward to form a hook-shaped or spoon-shaped structure. When leaf 5 is flipped outward and opened, the end of the hook-shaped / spoon-shaped structure hooks the outer periphery of the cell when leaf 5 is flipped back. This allows a single cloned stem cell to be easily scraped and pushed inward, and then the single cloned stem cell is wrapped in the space between multiple leaves 5.

[0037] By setting the distal end of leaf 5 to fold inward to form a hook-shaped or spoon-shaped structure, it is possible to easily propel the stem cell monoclonal molecule toward the center of the outer sheath 1.

[0038] Working principle: When using, hold the outer sheath tube 1 and press the end of the push-pull rod 4 with the pressing plate 3 to lower the push-pull rod 4. Through the connecting line 6, the blades 5 are flipped, and the channel at the bottom of the outer sheath tube 1 is opened. Align the bottom of the outer sheath tube 1 with the single clonal stem cell and lower it, surrounding the single clonal stem cell in the center of the multiple blades 5. Then, pull the pressing plate 3 upward to flip the blades 5 towards the center of the outer sheath tube 1. With the appropriate raising and lowering of the outer sheath tube 1, the five blades 5 are tightened and closed, and the bottom of the outer sheath tube 1 is closed. The single clonal stem cell is scooped from the container and placed in the outer sheath tube 1, completing the extraction process of the single clonal stem cell. The process of placing clonal stem cells is the same as the above procedure and will not be repeated here.

Claims

1. A portable, disposable stem cell monoclonal transfer device, characterized by, include: An outer sheath tube, wherein a clamping mechanism is provided at the front end of the outer sheath tube and a pressing plate is provided at the rear end of the outer sheath tube; The clamping mechanism includes multiple blades flexibly connected to the outer sheath tube. The blades are capable of flipping outward. A push-pull rod is installed inside the outer sheath tube. One end of the push-pull rod is fixedly connected to the pressing plate, and the other end of the push-pull rod is connected to the middle of the blade via a connecting line.

2. The portable, disposable stem cell monoclonal transfer device according to claim 1, characterized in that, The outer sheath tube is 10-15cm in length and 3-5mm in outer diameter.

3. The portable, disposable stem cell monoclonal transfer device of claim 1, wherein, The number of blades is five.

4. The portable, disposable stem cell monoclonal transfer device according to claim 1, characterized in that, An elastic component is provided inside the outer sheath, which enables the push-pull rod to automatically reset.

5. A portable, disposable stem cell monoclonal transfer device according to claim 4, characterized in that, The elastic component includes a fixing ring fixedly installed inside the outer sheath tube, a push-pull rod passing through the fixing ring, an integrally formed limiting ring on the push-pull rod, and a spring sleeved on the push-pull rod.

6. The portable, disposable stem cell monoclonal transfer device of claim 1, wherein, The multiple blades are stacked sequentially.

7. The portable, disposable stem cell monoclonal transfer device according to claim 1, characterized in that, The distal end of the blade folds inward to form a hook-shaped or spoon-shaped structure.

Images