ELECTROPHORESIS DEVICE

Abstract

To provide a simply structured electrophoresis device suitable for downsizing, a container can be prevented from lifting when a capillary is removed from the container, and the container can also be transferred properly. The electrophoresis device includes a capillary, a container (28) containing a sample or a reagent, a stage (34), and a drive section for driving the stage (34), as well as an engaging member (51L, 51R) capable of switching between an engaged state for engaging the container (28) with the stage (34) and a disengaged state, and a switching mechanism for switching the states of the engaging member (51L, 51R). Across a moving path of the stage (34), one side has a transfer section capable of transferring the container (28) to the stage (34).The other side has a connecting portion capable of connecting and disconnecting the capillary from the container (28). The switching mechanism switches the states of the engaging element (51L, 51R) in conjunction with the movement of the stage (34). The disengaged state is activated when the stage (34) is on one side. The engaged state is activated when the stage (34) is on the other side. The stage (34) moves horizontally while maintaining the engaged state on one side and the disengaged state on the other side.

Description

Technical area

[0001] The present invention relates to an electrophoresis device that performs electrophoresis in a capillary. State of the art

[0002] A capillary electrophoresis device that performs electrophoresis in a capillary is widely used as an electrophoresis device. The capillary is filled with a migration medium as an electrolyte and performs electrophoresis of a sample when voltage is applied to both ends. The capillary can be filled with a polymer gel or a polymer solution and used for capillary gel electrophoresis using the molecular sieve effect.

[0003] Generally, the capillary electrophoresis device comprises a capillary array integrating multiple capillaries or an autosampler. Electrophoresis samples are stored in a sample plate as a liquid container. The sample plate is available, for example, as a microtiter plate. The sample plate can store, for example, a buffer solution or a cleaning solution. The sample plate is placed on an autosampler stage and transported or manipulated in various ways.

[0004] Once placed on the stage, the sample plate is covered with a septum. The septum is a lid or membrane that prevents the liquid from evaporating and is generally made of elastomer. After being covered with the septum, the sample plate is attached to a plate holder and assembled into a sample plate assembly. The assembled sample plate assembly is placed on the autosampler stage.

[0005] The capillary array is positioned so that the tips of the capillaries protrude downward. After being placed on the autosampler stage, the sample plate assembly is transported horizontally and positioned beneath the capillary array. The stage is then driven to move upward. As the sample plate assembly moves upward, the capillary, whose relative position is fixed, penetrates the septum and is inserted, for example, into the sample plate well to aspirate the sample.

[0006] When the capillary is inserted into the septum, the closed opening elastically deforms and opens. As the capillary is inserted, the opening of the septum generates a restoring force in the direction of closing the opening. This seals the gap between the periphery of the capillary and the inner wall of the opening. When electrophoresis ends, the stage is driven to move downward. As the sample plate assembly moves downward, the capillary, whose relative position is fixed, is pulled out from the well interior above the septum.

[0007] When the capillary is pulled out of the septum, a frictional force is generated between the side of the capillary and the inner surface of the septum opening. This frictional force can lift the sample plate assembly placed on the stage. The lifted sample plate assembly may not be transported using the stage. To solve this problem, a method for attaching the sample plate assembly to the stage is considered.

[0008] The sample plate assembly attached to the stage must be capable of being detached when needed. In the field of electrophoresis devices, it is expected that multiple sample plate assemblies will be placed on the electrophoresis device for continuous analysis. When performing continuous analysis, the movable stage must not only transport the sample plate assembly but also be capable of transferring the sample plate assembly, such as detaching and replacing the sample plate assembly. To be transferred, the sample plate assembly must be detached at the transferred location above the stage.

[0009] Conventionally, various technologies have been proposed to detachably attach a container, such as a sample plate assembly for containing samples, to a movable stage.

[0010] According to the integrated sample processing system described in Patent Literature 1, "the components of the transport mechanism 5600 function as first and second releasable clamping mechanisms. The first releasable clamping mechanism exerts a force on the first surface (such as Y or X) of the microplate. The second releasable clamping mechanism exerts a force on a second surface (such as X or Y) of the microplate. The holder thereby holds the microplate from two sides. The clamping mechanism clamps the microplate between a positioning arm and an opposing frame structure. The positioning arm acts as an extrusion element. The opposing frame structure acts as a buffer for the clamping mechanism." (C47, L66)

[0011] According to the electrophoresis device described in Patent Literature 2, "the solenoid 304 controls the opening and closing operations of the electric gripper 127. When current flows through the solenoid 304, the solenoid 304 is driven, and the electric gripper 127 opens. When the current flowing through the solenoid 304 stops, the elastic force of the spring 305 closes the electric gripper 127. The electric gripper 127 opens when the sample container 122 is placed on the table 301. The electric gripper 127 closes after the sample container 122 is placed on the table 301. This makes it possible to hold the sample container 122." (paragraph 0037).

[0012] The electrophoresis device described in Patent Literature 3 "includes a capillary, a stage for placing a container containing a sample or a reagent, and an autosampler for moving the stage in a horizontal direction parallel to the surface of the stage and in a vertical direction perpendicular to the surface of the stage. The autosampler includes a stopper that applies a downward force to the container when the stage is lowered in the vertical direction to separate the container connected to the capillary from the capillary. Transferring the capillary places the container at a position where at least a part of the container faces the stopper." (paragraph 0007). Citation listPatent literature Patent Literature 1: US. Patent No. 6902703 Patent Literature 2: International Publication No. 2021 / 26951 Patent Literature 3: Japanese Unexamined Patent Application Publication No. 2022-035426 Summary of the inventionTechnical problem

[0013] In the field of electrophoresis devices, it is necessary to fix the container to the stage to prevent the container from lifting when the capillary is withdrawn from the container containing, for example, a sample. The container fixed to the stage must be automatically released at the container transfer point so that the container can be automatically removed from the stage or placed on the stage. The means to achieve these feats require a structure that does not occupy a large space around the container or a simple structure that can be implemented at low cost.

[0014] According to Patent Literature 1, the holder is positioned in a predetermined manner by applying a force to the microplate on two sides (X and Y) of the clamping mechanism. This method does not provide a solution to the upward reaction force generated by the downward distance from the capillary. The mechanism pushes the microplate laterally and requires a structure that slightly occupies the space around the container. There is a limitation on downsizing, which can reduce the projected area. When multiple microplates are positioned in parallel, it is difficult to reduce the distance between the microplates.

[0015] According to Patent Literature 2, an electric gripper driven by a solenoid is used to prevent the sample container from lifting. However, such an electric mechanism requires a power source and wiring, which are likely to occupy space within the electrophoresis device. The use of electric mechanisms makes it difficult to reduce costs and simplify structures.

[0016] According to Patent Literature 3, the sample adapter of the sample plate assembly includes a flange that engages the stopper and a groove that allows the upper surface of the stopper to pass through. However, such a structure breaks the compatibility of the sample plate assembly.

[0017] The present invention aims to provide a simply structured electrophoresis apparatus suitable for miniaturization, which can prevent a container from lifting when the capillary is removed from the container and also can transfer the container properly. Solution to the problem

[0018] To solve the above-described problem, an electrophoresis apparatus according to the present invention comprises the capillary; a container for containing a sample or a reagent; a stage for mounting the container; a driving portion for driving the stage at least in a horizontal direction; an engaging member capable of switching between an engaged state for engaging the container with the stage and a disengaged state for disengaging the container from the stage; and a switching mechanism that allows the engaging member to switch between the engaged state and the disengaged state. Across a moving path of the stage, one side has a transfer portion capable of transferring the container to the stage. The other side has a connecting portion capable of connecting and detaching the capillary to and from the container.A first position is provided between one side and the other side. A second position is provided between the first position and the other side. The switching mechanism allows the engaging member to enter the released state when the state of the engaging member transitions in conjunction with the movement of the stage between the first position and the second position to position the stage to one side. The engaging member enters the engaged state when the stage is positioned to the other side. The stage moves horizontally between one side and the first position while the engaging member is held in the engaged state. The stage moves horizontally between the other side and the second position while the engaging member is held in the released state. Advantageous effects of the invention

[0019] The present invention provides a simply structured electrophoresis apparatus suitable for miniaturization, which can prevent a container from lifting when a capillary is removed from the container and can also transfer the container properly. Short description of the drawings Fig. 1 is a schematic diagram illustrating the configuration of an electrophoresis apparatus according to an embodiment of the present invention. Fig. 2 is a perspective view illustrating the interior of the electrophoresis apparatus according to the embodiment of the present invention. Fig. 3 is a plan view illustrating the interior of the electrophoresis apparatus according to the embodiment of the present invention. Fig. 4 is a side view illustrating the interior of the electrophoresis apparatus according to the embodiment of the present invention. Fig. 5 is a perspective view illustrating a sample plate assembly. Fig. 6 is a perspective view illustrating an autosampler provided for the electrophoresis apparatus. Fig. 7 is an exploded perspective view illustrating a mechanism for opening and closing the holding portion. Fig. 8A is a perspective view illustrating the mechanism for opening and closing the holding portion in a closed state. Fig. 8B is a perspective view illustrating the mechanism for opening and closing the holding portion in an open state. Fig. Figure 9A is a cross-sectional view illustrating the autosampler when the holding section opening and closing mechanism is closed. Fig. Figure 9B is a cross-sectional view illustrating the autosampler when the holding section opening and closing mechanism is closed. Fig. Figure 10A is a diagram illustrating the process of transferring the sample plate assembly to a stage. Fig. 10B is a diagram illustrating the process of transferring the sample plate assembly to the stage. Fig. 11 is a diagram illustrating the operation of the holding section opening and closing mechanism equipped with a cam roller. Fig. 12 is a diagram illustrating the operation of the mechanism for opening and closing the holding section equipped with the cam roller. Fig. Figure 13 is a diagram illustrating the connection positions of the sample plate assembly connected with capillaries. Fig. 14 is a diagram illustrating the relationship between the position of the stage in the X-axis direction and the open-close state of the holding section. Fig. 15A is a diagram illustrating an exemplary shape of the holding portion. Fig. 15B is an enlarged view illustrating a relevant part of the exemplary shape of the holding portion. Fig. Figure 16 is a perspective view illustrating the placement of the autosampler and sample mounting section. Fig. 17 is an exploded perspective view of the mechanism for opening and closing the holding section equipped with a magnetic element. Fig. Figure 18 is a diagram illustrating the magnetic element and the magnetic poles acting as a driven side. Fig. Figure 19 is a diagram illustrating the magnetic element and the magnetic poles acting as a driving side. Fig. 20 is a diagram illustrating the operation of the mechanism for opening and closing the holding section equipped with the magnetic element. Fig. Fig. 21 is a perspective view illustrating the autosampler provided for the electrophoresis apparatus. Fig. 22A is a plan view illustrating the rotating mechanism in a released state. Fig. 22B is a plan view illustrating the rotating mechanism in an engaged state. Fig. 23 is a diagram illustrating the relationship between the stage position in the X-axis direction and the engagement state of an engagement blade. Description of the embodiments

[0020] The electrophoresis apparatus according to the embodiment of the present invention will be described below. In each drawing, the same reference numerals are used to denote similar configurations, and duplicate descriptions are omitted.

[0021] Fig. 1 is a schematic diagram illustrating the configuration of an electrophoresis apparatus according to an embodiment of the present invention.

[0022] As in Fig. As illustrated in Figure 1, an electrophoresis device 1 includes, for example, a capillary assembly 2, an electrophoresis section 3, a liquid supply section 4, and an irradiation detection section 5. The electrophoresis device 1 is a capillary electrophoresis device that performs electrophoresis in a capillary. The electrophoresis device 1 can be used to analyze base sequences of nucleic acids, such as DNA, and ions.

[0023] The capillary assembly 2 is formed, for example, by assembling a plurality of capillaries 6, a capillary head 7, a loading head 8, and a clamping plate 9. The capillary assembly 2 can be provided as a detachable capillary assembly unit. The capillary assembly unit can be attached and detached, thereby making it possible to easily replace the capillary 6 when the capillary 6 is used for a predetermined number of analyses or when analysis objectives or items are changed.

[0024] Capillary 6 is a narrow tube and is generally formed by coating a narrow tube made of quartz glass with polyimide. The coating is used to ensure resistance to damage such as cracks. The coating is removed in the area corresponding to a detection position 12 of capillary 6.

[0025] The capillary head 7 is attached to a block 17 of the liquid supply section 4. The capillary head 7 bundles several capillaries 6 and connects the anode end of the capillary 6 to the block 17 of the liquid supply section 4.

[0026] The charging head 8 is attached to the bottom of the electrophoresis section 3. The charging head 8 clamps the plurality of capillaries 6 and a cathode electrode 10. The capillary 6 is clamped to the charging head 8 to penetrate the cathode electrode 10. The capillary 6 has a cathode end 11 that protrudes downward from the charging head 8.

[0027] The clamping plate 9 is attached to the irradiation detection section 5. The clamping plate 9 has a plurality of grooves for aligning the capillaries 6. The clamping plate 9 aligns and clamps the plurality of capillaries 6. The clamping plate 9 removes the coating from a portion of the capillary 6. This portion is positioned on the optically flat surface of the detection position 12 with a precision of several micrometers.

[0028] The electrophoresis section 3 performs electrophoresis using the capillary assembly 2. The electrophoresis section 3 includes, for example, a thermostatic bath 83, a cathode-side buffer tank 13, and a high-voltage power supply 14.

[0029] The thermostatic bath 83 houses an intermediate portion of the capillaries 6 that form the capillary array 2. The interior of the thermostatic bath 83 is set to a predetermined temperature. For example, a Peltier element is used as the heat source of the thermostatic bath 83. The thermostatic bath 83 can be set to a temperature ranging from a temperature lower than room temperature to a high temperature of 50°C or higher. The thermostatic bath 83 can include a fan (not shown). The fan can circulate the air inside and reduce spatial temperature fluctuations.

[0030] The cathode-side buffer container 13 stores a buffer solution 15. The cathode-side buffer container 13 is designed to be movable relative to the cathode end 11 and the cathode electrode 10 of the capillary 6. The cathode end 11 and the cathode electrode 10 of the capillary 6 are immersed in the buffer solution 15 during electrophoresis.

[0031] The high-voltage supply 14 is electrically connected to the cathode electrode 10. The high-voltage supply 14 applies a high voltage between the cathode electrode 10 and an anode electrode 21 during electrophoresis. When applied, the voltage can move an electrical charge within the capillary 6, thereby generating an electroosmotic flow.

[0032] The liquid supply section 4 supplies a migration medium into the interior of the capillary 6. The liquid supply section 4 comprises, for example, a pump 16, the block 17, a polymer container 18 and an anode-side buffer container 19. As the migration medium, for example, a highly viscous polymer solution 20 is used.

[0033] The pump 16 is connected to the block 17 of the liquid supply section 4. The pump 16 delivers the migration medium prepared in the polymer container 18 into the interior of the capillary 6.

[0034] Block 17 includes an internal flow path. Block 17 connects the capillary head 7, the pump 16, the polymer reservoir 18, and the anode-side buffer reservoir 19 via the internal flow path.

[0035] The polymer container 18 stores the polymer solution 20 as a migration medium. The pump 16 operates to fill the polymer solution 20 stored in the polymer container 18 through the block 17 into the capillary 6.

[0036] The anode-side buffer tank 19 stores a buffer solution 15. The anode electrode 21 is immersed in the buffer solution 15. The cathode end 11 of the capillary 6 is immersed in the buffer solution 15 contained in the cathode-side buffer tank 13. Then, the buffer solution 15 contained in the anode-side buffer tank 19 and the buffer solution 15 contained in the cathode-side buffer tank 13 are electrically connected via the polymer solution 20 filled into the capillary 6.

[0037] The irradiation detection section 5 optically detects the components separated by electrophoresis within the capillary 6. The irradiation detection section 5 comprises, for example, a light source 22 and a detector 23.

[0038] The light source 22 generates excitation light of a specific wavelength and radiates it onto the detection position 12 of the capillary 6. The coating of the capillary 6 is removed at the detection position 12, allowing light to pass through. The excitation light generated by the light source 22 is radiated onto components within the capillary 6 that have been separated by electrophoresis.

[0039] The detector 23 detects the light emitted by components separated by electrophoresis. The sample can be labeled with a fluorescent dye. Then, the components irradiated with the excitation light emit light such as fluorescence. The excitation light can be irradiated onto the components passing through the detection position 12, and the light emitted by the components can be detected. It is possible to detect and quantify the components separated by electrophoresis based on any size.

[0040] A control device (not shown) controls the operations of each component of the electrophoresis device 1. The control device consists of, for example, a processor for executing a program for operating the electrophoresis device 1 and a memory for storing the program.

[0041] Fig. 2 is a perspective view illustrating the interior of the electrophoresis apparatus according to the embodiment of the present invention. Fig. 3 is a plan view illustrating the interior of the electrophoresis apparatus according to the embodiment of the present invention. Fig. 4 is a side view illustrating the interior of the electrophoresis apparatus according to the embodiment of the present invention. Fig. 2, Fig. 3 and Fig. 4 include partial perspective views of an example inside the electrophoresis apparatus. Fig. 3 corresponds to a view from direction A in Fig. 2. Fig. 4 corresponds to a view from direction B in Fig. 2.

[0042] As in Fig. 2, Fig. 3 and Fig. 4, the electrophoresis device 1 comprises a housing 24, an autosampler 25, a buffer transport section 26 and a sample mounting section 27, for example, in addition to the parts shown in Fig. 1. The control device (not shown) controls the autosampler 25 and the buffer transport section 26 as shown in Fig. 1 illustrated components.

[0043] The housing 24 contains the main components, such as the capillary assembly 2, the electrophoresis section 3, the liquid supply section 4, the irradiation detection section 5, the autosampler 25, the buffer transport section 26, and the sample mounting section 27. The housing 24 may also include an openable door for maintenance or replacement of the components.

[0044] The autosampler 25 is provided at the rear of the electrophoresis device 1. The autosampler 25 transports a sample plate assembly 28, for example, to be able to aspirate and withdraw samples. The autosampler 25 is provided such that the sample plate assembly 28 can be moved bidirectionally along three mutually orthogonal axial directions.

[0045] The buffer transport section 26 is provided at the rear of the electrophoresis apparatus 1. The buffer transport section 26 transports the cathode-side buffer container 13. The buffer transport section 26 is provided so that the cathode-side buffer container 13 can be moved bidirectionally along at least two axial directions, horizontally and vertically.

[0046] In this description, the X-axis direction and the Y-axis direction correspond to the horizontal direction and correspond to the direction parallel to the mounting surface of a stage 34 on which the sample plate assembly 28 is placed. The Z-axis direction corresponds to the vertical direction and corresponds to the direction perpendicular to the mounting surface of the stage 34 on which the sample plate assembly 28 is placed. The X-axis direction also corresponds to the forward-backward direction of the electrophoresis device 1. The positive direction indicates the "front" and the negative direction indicates the "back." The Y-axis direction also corresponds to the horizontal direction of the electrophoresis device 1. The positive direction means "right" and the negative direction means "left." The Z-axis direction also corresponds to the vertical direction of the electrophoresis device 1. The positive direction means "up" and the negative direction means "down."

[0047] The sample mounting section 27 allows a user to mount the sample plate assembly 28 from outside the electrophoresis device 1. The sample mounting section 27 is provided at the front of the electrophoresis device 1. The sample mounting section 27 includes a slotted section 40 for individually placing the sample plate assembly 28.

[0048] In a planar view, the slotted portion 40 is shaped into a groove slightly larger than the outer shape of the sample plate assembly 28. A receiving plane is formed toward the bottom of the slotted portion 40. The receiving plane protrudes inward from the right and left sides of the slotted portion 40. The lower end of the slotted portion 40 is open except for the receiving plane. The rear end of the slotted portion 40 is open.

[0049] The sample plate assembly 28, which contains samples, for example, is placed in the slotted section 40 to be supported from below by the receiving plane. Since the lower and rear ends of the slotted section 40 are open, the table 34 of the autosampler 25 can easily approach the sample plate assembly 28 placed in the slotted section 40.

[0050] As can be seen from the Fig. 2, Fig. 3 and Fig. As shown in Figure 4, the sample mounting section 27 is provided like a drawer. A pair of right and left sample mounting section rails 41 support the sample mounting section 27 for movement in the front-back direction. The sample mounting section 27 includes the plurality of slot sections 40 provided in parallel to accommodate a total of four sample plate assemblies 28. The sample mounting section 27 can be configured to accommodate any number of sample plate assemblies 28.

[0051] Fig. 5 is a perspective view illustrating a sample plate assembly. Fig. 5 illustrates the sample plate assembly 28 as a container for samples, for example, with the components separated from each other.

[0052] As in Fig. 5, the sample plate assembly 28 is constructed of a sample adapter 29, a sample plate 30, a septum 31, and a septum clip 32. The user assembles the sample adapter 29, the sample plate 30, the septum 31, and the septum clip 32 in this order from the bottom so that they are stacked.

[0053] The sample adapter 29 supports the sample plate 30 and facilitates the placement of the sample plate 30 on the stage 34 of the electrophoresis device 1. The sample adapter 29 is provided as a fitting for the electrophoresis device 1 to ensure proper positioning on the stage 34 of the autosampler 25.

[0054] The sample plate 30 is a container that stores, for example, samples and reagents. The sample plate 30 represents a container referred to, for example, as a microtiter plate or a microtiter plate. Such a container includes a plurality of wells arranged in a rectangular matrix to be able to contain, for example, liquids.

[0055] The septum 31 is a lid or membrane that covers the sample plate 30. The septum 31 has a bottomed cylindrical portion that protrudes downward at a position corresponding to the well of the sample plate 30. The septum 31 is attached to the sample plate 30 so that the cylindrical portion fits into the well. The bottom of the cylindrical portion has an opening through which the capillary 6 can be inserted. The septum 31 can cover the well while allowing the capillary 6 to be inserted and removed, thereby preventing the liquid contained in the well from evaporating before and after analysis.

[0056] The septum 31 is advantageously made of an elastomer, such as silicone rubber. When the capillary 6 is inserted into the opening, the elastomer can expand the opening due to elastic deformation and exert a restoring force in a direction toward closing the opening. The well can remain sealed at a high level regardless of whether the capillary 6 is inserted into the opening.

[0057] The septum clip 32 secures the sample plate 30 and the septum 31 to the sample adapter 29. The septum clip 32 is attached to the sample adapter 29 to cover the sample plate 30 and the septum 31 from above. The septum clip 32 has an opening at a position corresponding to the well of the sample plate 30. The cathode end 11 of the capillary 6 is inserted through the opening of the septum clip 32 into the interior of the well of the sample plate 30.

[0058] The septum clip 32 includes a nail 92. The nail 92 protrudes downward from both ends of the septum clip 32 in the front-back direction. A nail catch is provided at both ends of the sample adapter 29 in the front-back direction to be able to engage with the nail 92. When the nail 92 engages with the nail catch, the septum clip 32 can fix the sample plate 30 and the septum 31 to the sample adapter 29 between them.

[0059] The sample adapter 29 is externally provided in an approximately rectangular parallelepiped shape. The sample adapter 29 has a top surface for placing the sample plate 30, a pair of right and left side surfaces parallel to the front-back direction, a front surface parallel to the horizontal direction, and a back surface parallel to the horizontal direction. The sample adapter 29 is externally shaped in an approximately rectangular parallelepiped, the bottom surface of which is concave and open upward.

[0060] The sample adapter 29 has a holding pin engagement hole 33R on the right side and a holding pin engagement hole 33L (not shown) on the left side. The holding pin engagement holes 33L and 33R are formed as through holes that penetrate the sides of the sample adapter 29 inward and outward. The bottom surface of the sample adapter 29 is open, allowing the outer side of the right and left side surfaces of the sample adapter 29 to communicate with the lower part of the sample adapter 29 through the holding pin engagement holes 33L and 33R.

[0061] Holding nails 67L and 67R are inserted into the holding nail engagement holes 33L and 33R to be engaged. When the holding nail engagement holes 33L and 33R are engaged with the holding nails 67L and 67R, the sample plate assembly 28 placed on the stage 34 is fixed to the stage 34. The holding nail engagement holes 33L and 33R are advantageously shaped into rectangles such that the width is longer than the height, corresponding to the shape of the holding nails 67L and 67R.

[0062] The sample plate assembly 28 acts, for example, as a container for storing samples and provides a unit that is automatically transported within the electrophoresis device 1. When multiple sample plate assemblies 28 are used for analysis in the electrophoresis device 1, the multiple assembled sample plate assemblies 28 are placed on the sample mounting section 27. Transport by the autosampler 25 and electrophoresis through the capillary assembly 2 are performed in units of the sample plate assembly 28. <elektrophoreseanalyseverfahren>

[0063] The following describes the electrophoresis analysis method for samples stored in the sample plate assembly 28 of the electrophoresis apparatus 1.

[0064] To electrophoretically analyze a sample, the autosampler 25 is first driven to move the sample plate assembly 28, which is placed on the sample mounting section 27, onto the stage 34 of the autosampler 25. Then, the stage 34, mounted with the sample plate assembly 28, is transported under the capillary assembly 2 and then lifted toward the cathode end 11 of the capillary 6.

[0065] The stage 34 raises the sample plate assembly 28 to a height at which the cathode end 11 of the capillary 6 is immersed in the sample contained in the well of the sample plate 30. The cathode end 11 of the capillary 6, whose relative position is fixed, passes through the opening of the septum 31 and is inserted into the interior of the well of the sample plate 30 to aspirate the sample in the well.

[0066] A voltage is applied between the cathode electrode 10 and the anode electrode 21 to introduce samples into the capillary 6 and perform electrophoresis to separate samples. The high-voltage power supply 14 applies a high voltage of several kilovolts between the cathode electrode 10 and the anode electrode 21.

[0067] The applied voltage introduces the sample prepared in the sample plate 30 into the capillary 6. The autosampler 25 is then driven to lower the stage 34, which is mounted with the sample plate assembly 28, below the capillary assembly 2 and remove the cathode end 11 of the capillary 6 from the sample plate assembly 28.

[0068] Then, the buffer transport section 26 is driven to transport the cathode-side buffer container 13 containing the buffer solution 15 under the capillary assembly 2 and then lift it toward the cathode end 11 of the capillary 6. The cathode end 11 of the capillary 6 is inserted into the cathode-side buffer container 13 and immersed in the buffer solution 15.

[0069] While the cathode end 11 is immersed in the buffer solution 15, the application of a voltage causes the sample introduced into the capillary 6 to migrate in units of components toward the anode end of the capillary 6 due to the charge on the sample components and an electroosmotic flow. The molecular sieve effect due to the polymer is achieved within the capillary 6 filled with the polymer solution 20, causing differences in the migration rate depending on the size of each component. For example, the migration rate of DNA increases as the molecular length decreases, and it decreases as the molecular length increases.

[0070] Differences in migration speed in the capillary 6 separate the components contained in the sample according to their sizes. Each component contained in the sample can be labeled, for example, with a fluorescent marker. The irradiation detection section 5 can then detect the optical intensity of each separated component. <Konfiguration des Autosamplers>

[0071] The following describes the configuration of the autosampler 25 included in the electrophoresis apparatus 1.

[0072] Fig. 6 is a perspective view illustrating an autosampler provided for the electrophoresis apparatus. Fig. 6 shows a partially sectioned view of an example structure of the autosampler 25 included in the electrophoresis apparatus 1.

[0073] As in Fig. 6, the autosampler 25 includes, for example, a sliding section 50 that moves together with the table 34, an X-axis driving section 35, a Z-axis driving section 36, and a Y-axis driving section 37.

[0074] The stage 34 serves as a mounting point for transporting the sample plate assembly 28. The stage 34 is supported above a stage base 42. The stage 34 and the stage base 42 form, for example, the sliding section 50, which moves integrally along the X-axis direction. The stage 34 is formed into a flat plate with a flat upper surface. The autosampler 25 is driven to automatically transfer the sample plate assembly 28, which is placed on the sample mounting section 27, to the stage 34.

[0075] The X-axis drive section 35 drives the slide section 50 bidirectionally in the X-axis direction. The X-axis drive section 35 transports the stage 34 in the forward-backward direction. The X-axis drive section 35 is attached to the Z-axis drive section 36. The X-axis drive section 35 includes a plate-like X-axis drive base 43 extending parallel to the XY plane. An X-axis guide rail 44 is installed on top of the X-axis drive base 43 to extend along the X-axis direction. The slide section 50 is guided along the X-axis guide rail 44.

[0076] A drive source 45 is provided at one end of the X-axis drive base 43. The drive source 45 consists, for example, of a stepping motor that drives the stepping motion. A pulley is provided at the other end of the X-axis drive base 43. An X-axis drive belt 46 runs between the output shaft of the drive source 45 and the pulley along the X-axis direction. A drive link 48, which is connected to the sliding portion 50, is attached to the X-axis drive belt 46. The rotational movement of the X-axis drive belt 46 drives the sliding portion 50 to move along the X-axis guide rail 44.

[0077] The Z-axis drive section 36 drives the slide section 50 and the X-axis drive section 35 in the Z-axis direction. The Z-axis drive section 36 transports the stage 34 in the vertical direction. The Z-axis drive section 36 is fixed to the Y-axis drive section 37. The Z-axis drive section 36 includes a plate-like body extending parallel to the ZX plane. A Z-axis guide rail 39 is installed on the main surface of the body to extend along the Z-axis direction. The X-axis drive section 35 is guided along the Z-axis guide rail 39.

[0078] A drive source 81 is provided at one end of the Z-axis drive section 36. The drive source 81 consists of a stepping motor that drives the stepping motion. A pulley is provided at the other end of the Z-axis drive section 36. A Z-axis drive belt 82 runs between the output shaft of the drive source 81 and the pulley along the Z-axis direction. A drive link (not shown) connected to the sliding section 50 is attached to the Z-axis drive belt 82. The rotational movement of the Z-axis drive belt 82 drives the sliding section 50 to move along the Z-axis guide rail 39.

[0079] The Y-axis drive section 37 drives the slide section 50, the X-axis drive section 35, and the Z-axis drive section 36 in the Y-axis direction. The Y-axis drive section 37 transports the stage 34 in the horizontal direction. The Y-axis drive section 37 includes a plate-like body extending parallel to the XY plane. A Y-axis guide rail 38 is installed on the main surface of the body to extend along the Y-axis direction. The Y-axis drive section 37 is guided along the Z-axis guide rail 39. The slide section 50 is driven to move along the Y-axis guide rail 38 according to a mechanism similar to the X-axis drive section 35 and the Z-axis drive section 36.

[0080] The X-axis drive section 35, the Z-axis drive section 36, and the Y-axis drive section 37 transport the stage 34 through the X-axis zone between the sample mounting section 27 and the space under the capillary assembly 2, the Y-axis zone for the sample mounting section 27, the Z-axis zone for the sample mounting section 27, and the Z-axis zone under the capillary assembly 2. The sample mounting section 27 lifts the sample plate assembly 28, which is placed in the slot section 40, from below for the purpose of transfer.

[0081] A positioning pin 49 is provided on the top of the stage 34 to protrude upward. The positioning pin 49 is designed to engage with a positioning hole in the bottom of the sample adapter 29. When the sample plate assembly 28, which is placed in the slot portion 40, is lifted from below, the engagement between the positioning pin 49 and the positioning hole positions the sample plate assembly 28 on the stage 34.

[0082] The sample plate assembly 28 is transferred to the stage 34, transported horizontally toward the bottom of the capillary array 2, and then transported vertically upward toward the cathode end 11 of the capillary 6. As the sample plate assembly 28, mounted on the stage 34, moves upward, the capillary 6 penetrates the septum 31 and is inserted into the recess of the sample plate 30.

[0083] When the capillary 6 is inserted, the opening of the septum 31 expands due to elastic deformation, connecting the inside and outside of the well. When the capillary 6 is inserted into the opening of the septum 31, a restoring force is generated in the direction of closing the opening, sealing the gap between the side surface of the capillary 6 and the inner wall of the opening in the septum 31. When electrophoresis ends, the stage 34 moves downward, and the sample plate assembly 28 is pulled out from under the cathode end 11 of the capillary 6.

[0084] When the capillary 6 is pulled out of the opening of the septum 31, a frictional force is generated between the side of the capillary 6 and the inner surface of the opening of the septum 31. This frictional force can lift the sample plate assembly 28 above the stage 34. By securing the sample plate assembly 28 to the stage 34, the sample plate assembly 28 can be prevented from lifting.

[0085] By securing the sample plate assembly 28 to the stage 34, the sample plate assembly 28 can be prevented from lifting. However, the sample mounting section 27 must mount the sample plate assembly 28 from the stage 34 to the slotted section 40 and from the slotted section 40 to the stage 34. The sample plate assembly 28 must be released to be freely mounted.

[0086] According to the present embodiment, the electrophoresis apparatus 1 employs a mechanism that can detachably attach the sample plate assembly 28 to the stage 34. The sample plate assembly 28 may be misaligned in the X-axis direction at the sample mounting portion 27 located at one end of the X-axis movement path of the stage 34. It would be advantageous to increase a high degree of freedom for positions in the X-axis direction. The mechanism for attaching the sample plate assembly 28 must be activated and deactivated at the center of the X-axis movement path of the stage 34.

[0087] The mechanism for securing the sample plate assembly 28 includes an engagement member that engages and secures the sample plate assembly 28 to the side of the stage 34. The engagement member can switch between an engaged state in which the sample plate assembly 28 is engaged with the stage 34 and a disengaged state in which the sample plate assembly 28 is disengaged from the stage 34. The engagement member is installed on the sliding portion 50 to fix the relative position with respect to the stage 34.

[0088] A switching mechanism is provided to switch the engagement element between the engaged state and the released state. The switching mechanism is configured to switch the states of the engagement element in conjunction with the movement of the stage 34, so that the sample plate assembly 28 can be attached to and detached from the stage 34 without the use of additional power. <Erste Ausführungsform>

[0089] According to the first embodiment, the electrophoresis device 1 uses the engaging element represented by holding sections 51L and 51R, which rotate around a central axis parallel to the horizontal direction to open and close. The switching mechanism for switching the states of the engaging element uses a cam mechanism that operates in conjunction with the movement of the stage 34. The cam mechanism driver uses a cam member 72 having an inclined cam surface 73 provided on the movement path of the stage 34 in the X-axis direction. The cam mechanism follower uses a holding section opening and closing mechanism 47, which includes a cam roller 60 that acts as a cam follower. The holding section opening and closing mechanism 47 includes the holding sections 51L and 51R and the cam roller 60, and moves together with the stage 34.

[0090] Fig. 7 is an exploded perspective view illustrating a mechanism for opening and closing the holding portion. Fig. 8A is a perspective view illustrating the mechanism for opening and closing the holding portion in a closed state. Fig. 8B is a perspective view illustrating the mechanism for opening and closing the holding portion in an open state. Fig. 7 is an exploded perspective view illustrating the holding portion opening and closing mechanism 47 including the holding portions 51L and 51R and the cam roller 60. Fig. 8A and Fig. 8B illustrate the sliding portion formed by mounting the holding portion opening and closing mechanism 47 on the stage base 42.

[0091] As in Fig. 7, Fig. 8A and Fig. 8B illustrates, illustrate Fig. 8A and Fig. 8B shows the sliding portion formed by mounting the holding portion opening and closing mechanism 47 on the stage base 42. According to the first embodiment, the holding portion opening and closing mechanism 47 includes the cam roller 60 acting as the cam mechanism follower.

[0092] The holding section opening and closing mechanism 47 is formed by assembling the left holding section 51L, the right holding section 51R, and a swing member 56 to a holding section base member 52 serving as the base. The holding section base member 52 is fixed to the upper surface of the stage base 42. The stage 34 is supported above the stage base 42 by a support post erected on the upper surface of the stage base 42. The holding section opening and closing mechanism 47 is provided below the stage 34 and fixes the sample plate assembly 28 mounted on the stage 34 from below.

[0093] A pair of left and right first support shaft holes 53L and 53R are provided on the rear side of the holding section base member 52. To penetrate in the horizontal direction, the first support shaft holes 53L and 53R are drilled into upward parts of the holding section base member 52. The first support shaft holes 53L and 53R are provided approximately symmetrically to the right and left.

[0094] The first support shaft holes 53L and 53R are provided with lubricating bushings 54L and 54R, respectively, into which a first support shaft 55 is inserted. The first support shaft 55 is rotatably supported on the rear side of the holding section base member 52. The first support shaft 55 is provided rotatably about a central axis parallel to the horizontal direction. A retaining ring (not shown), for example, prevents the first support shaft 55 from slipping.

[0095] The oscillating member 56 is supported above the holding portion base member 52. Pressure portions 61L and 61R are provided at the front of the oscillating member 56. The cam roller 60 is fixed to the rear of the oscillating member 56. A second support shaft hole 57 is provided at the center of the oscillating member 56 in the front-back direction. To penetrate in the horizontal direction, the second support shaft hole 57 is drilled into downward parts of the oscillating member 56.

[0096] The first support shaft 55 is inserted into the second support shaft hole 57. The swing member 56 is rotatably supported by the first support shaft 55 parallel to the horizontal direction. The swing member 56 is pivotally provided using the first support shaft 55 as a pivot point, so that one side and the other side rock around the first support shaft 55.

[0097] A cam roller pin hole 58 is provided on the back of the oscillating member 56. To penetrate in the horizontal direction, the cam roller pin hole 58 is drilled into the downward-facing parts of the oscillating member 56. A cam roller pin 59 is fixed to the cam roller pin hole 58. The cam roller 60 is fixed to the cam roller pin 59 by welding or forging. The cam roller 60 is rotatable about a central axis parallel to the horizontal direction, provided on the back of the oscillating member 56.

[0098] At the front, the swing member 56 includes a planar portion formed to extend in the horizontal direction and the front-back direction to form a T-shape when viewed from above. The left side of this portion acts as the pressing portion 61L, which presses one end of the left holding portion 51L. The right side of this portion acts as the pressing portion 61R, which presses one end of the right holding portion 51R.

[0099] The pressing portions 61L and 61R press the ends of the holding portions 51L and 51R, thereby mechanically attaching and detaching the sample plate assembly 28 from the stage 34. The pressing portions 61L and 61R are advantageously positioned higher than the second support shaft hole 57. According to this positioning, the pressing portions 61L and 61R can generate a large moment when the oscillating member 56 oscillates.

[0100] A stopper 62 is provided at the front of the holding section base member 52. The stopper 62 is provided to extend rearward from a predetermined height relative to the top of the holding section base member 52. The stopper 62 limits the upper limit of the height of the pressing sections 61L and 61R operating in the vertical direction. The pressing sections 61L and 61R rise to a predetermined height and contact the stopper 62, thereby limiting the range of swinging movement of the swinging member 56.

[0101] A compression spring 63' is provided at the center of the holding section base member 52 in the front-back direction. One end of the compression spring 63 is fixed to the top of the holding section base member 52. The other end of the compression spring 63 is fixed to the bottom of the swing member 56, behind the pressing sections 61L and 61R and in front of the second support shaft hole 57. The compression spring 63 is installed between the top of the holding section base member 52, in front of the first support shaft holes 53L and 53R, and the bottom of the swing member 56, in front of the second support shaft hole 57.

[0102] The compression spring 63 elastically supports the front of the oscillating member 56. When the cam roller 60 can descend, the compression spring 63 exerts force to raise the front of the oscillating member 56. This force keeps the pressing portions 61L and 61R in contact with the stopper 62. The state of the oscillating member 56 can be stabilized. When the cam roller 60 rises, the front of the oscillating member 56 descends to press the compression spring 63. In this state, the pressing portions 61L and 61R can press one end of the holding portions 51L and 51R.

[0103] Third support shaft holes 64L and 64R are provided for the front side of the holding section base member 52 at the left and right side ends, respectively. The left third support shaft hole 64L and the right third support shaft hole 64R are each formed of a pair of through holes spaced from each other in the front-back direction. The third support shaft holes 64L and 64R are drilled in the front-back direction to penetrate an upward part of the holding section base member 52. The third support shaft holes 64L and 64R are provided approximately symmetrically to the right and left.

[0104] Second support shafts 65L and 65R are inserted into the third support shaft holes 64L and 64R, respectively. The second support shafts 65L and 65R are rotatably supported at the left and right side ends on the front side of the holding section base member 52. The second support shafts 65L and 65R are provided rotatably about a central axis parallel to the front-rear direction. The second support shafts 65L and 65R rotatably support the holding sections 51L and 51R.

[0105] The holding sections 51L and 51R are provided bilaterally symmetrically in terms of shape, structure, and operation, so that they form a pair on the left and right sides. Fig. 7, the holding portions 51L and 51R are each provided such that a flat plate shaped like a rectangular parallelepiped is butt-bent. Fourth support shaft holes 66L and 66R are provided in the bent intermediate part of the holding portions 51L and 51R. The fourth support shaft holes 66L and 66R are drilled to penetrate in the front-back direction.

[0106] The second support shafts 65L and 65R are inserted into the fourth support shaft holes 66L and 66R, respectively. The second support shafts 65L and 65R rotatably support the holding portions 51L and 51R, respectively. The holding portions 51L and 51R are rotatably provided around the second support shafts 65L and 65R, so that one end and the other end clamping the second support shafts 65L and 65R alternately ascend and descend.

[0107] The holding nails 67L and 67R are formed at one end of the holding portions 51L and 51R. The holding nails 67L and 67R are provided as nail-shaped projections projecting from the side surface of one end of the holding portions 51L and 51R so as to be capable of projecting laterally or downwardly from one end of the holding portions 51L and 51R.

[0108] The holding nails 67L and 67R are formed linearly along the front-back direction to correspond to the holding nail engagement holes 33L and 33R provided on the side surface of the sample adapter 29. The holding nails 67L and 67R are formed with flat bottom surfaces that can contact the lower inner walls of the holding nail engagement holes 33L and 33R. The holding nails 67L and 67R are inserted into and engaged with the holding nail engagement holes 33L and 33R, acting as a displacement suppression portion that prevents the sample adapter 29 from being displaced upward.

[0109] The holding portions 51L and 51R include spring hook portions 68L and 68R and pressure protrusion portions 70L and 70R formed at the other end. The spring hook portions 68L and 68R are shaped like rods and are installed in the front-back direction. The pressure protrusion portions 70L and 70R are provided outside the spring hook portions 68L and 68R. The pressure protrusion portions 70L and 70R are shaped into protrusions that protrude upward so as not to obstruct the surroundings.

[0110] A tension spring 69 is stretched between the spring hook portions 68L and 68R. The tension spring 69 exerts a force in a direction to bring the inner ends of the right and left holding portions 51L and 51R close to each other. This force exerts a lateral protrusion action of the holding nails 67L and 67R provided outside the holding portions 51L and 51R. The action protrudes upwardly of the pressing protrusion portions 70L and 70R provided inside the holding portions 51L and 51R.

[0111] The pressing portions 61L and 61R press the pressing protrusion portions 70L and 70R. The pressing protrusion portions 70L and 70R are positioned to overlap the pressing portions 61L and 61R according to a planar view of the mechanism for opening and closing the holding portion 47. The tip surfaces of the pressing protrusion portions 70L and 70R are advantageously curved like a sphere. This curved surface can generate a high suppression strength in the direction of changing the opening degrees, regardless of the opening degrees of the holding portions 51L and 51R.

[0112] The oscillating member 56 oscillates so that one side and the other side, which clamps the first support shaft 55, alternately ascend and descend. The front side of the oscillating member 56 ascends to lower the rear side. The front side of the oscillating member 56 descends to raise the rear side. When the cam roller 60 ascends, the pressing portions 61L and 61R descend, pressing the pressing protrusion portions 70L and 70R of the holding portions 51L and 51R. When the cam roller 60 descends, the pressing portions 61L and 61R rise, releasing the pressure on the pressing protrusion portions 70L and 70R.

[0113] The holding portions 51L and 51R can switch between a closed state and an open state according to rotation about the second support shafts 65L and 65R. In the closed state, the outer side where the holding nails 67L and 67R are formed is directed upward. In the open state, the outer side where the holding nails 67L and 67R are formed inclines inward to be positioned further inward than upward.

[0114] The pressing portions 61L and 61R rise to a predetermined height, contact the stopper 62, and do not rise any higher. The maximum height of the pressing projection portions 70L and 70R is limited to the position in contact with the pressing portions 61L and 61R, which contact the stopper 62. The maximum height of the pressing projection portions 70L and 70R corresponds to the maximum opening degree of the holding portions 51L and 51R. The opening degree of the holding portions 51L and 51R can be adjusted by adjusting the height of the stopper 62.

[0115] As in Fig. As illustrated in Figure 8A, when the cam roller 60 descends, the front side of the swing member 56 rises to release the pressure on the pressing protrusion portions 70L and 70R caused by the pressing portions 61L and 61R. Consequently, the outer side of the holding portions 51L and 51R stands upright and faces upward. This state corresponds to the closed state of the holding portions 51L and 51R.

[0116] When the holding sections 51L and 51R remain in the closed state, the holding nails 67L and 67R are inserted into the holding nail engagement holes 33L and 33R of the sample adapter 29 to enter an engagement state. In the closed state, the inner ends of the holding sections 51L and 51R are directed inward to be horizontal or are lowered inward to slightly tilt. In the closed state, the sample plate assembly 28, which is placed on the stage 34, is fixed to the stage 34.

[0117] As in Fig. As illustrated in Figure 8B, when the cam roller 60 rises, the front side of the oscillating member 56 descends. The pressing portions 61L and 61R press the pressing protrusion portions 70L and 70R. As a result, the outer sides of the holding portions 51L and 51R tilt and are directed inward. This state corresponds to the open state of the holding portions 51L and 51R.

[0118] When the holding portions 51L and 51R are in the open state, the holding nails 67L and 67R are in the released state, so they can be detached from the holding nail engagement holes 33L and 33R of the sample adapter 29. In the open state, the inner ends of the holding portions 51L and 51R are lowered inward to tilt deeply. In the open state, the sample plate assembly 28 is detached from the stage 34.

[0119] The amount of displacement of each component of the holding section opening and closing mechanism 47 depends on the amount of displacement of the cam roller 60 in the vertical direction within the range regulated by the stopper 62. Each component of the holding section opening and closing mechanism 47 can transition to an intermediate state while the holding sections 51L and 51R switch between the closed state and the open state. The amount of displacement of the cam roller 60 in the vertical direction is changed by the cam member 72, which has the inclined cam surface 73 provided on the moving path of the stage 34 in the X-axis direction.

[0120] The main structure of the holding section opening and closing mechanism 47 can be made of a suitable material such as steel, carbon steel, stainless steel, or an aluminum alloy. The holding sections 51L and 51R can also be formed by resin molding. It is preferable to use polyacetal, for example, as the resin due to its excellent wear resistance and sliding properties. <Transfervorgang der Probenplattenbaugruppe>

[0121] The following describes the operation of transferring the sample plate assembly 28 to the stage 34 and the cam member 72 provided on the moving path of the stage 34 in the X-axis direction.

[0122] Fig. Figure 9A is a cross-sectional view illustrating the autosampler when the holding section opening and closing mechanism is closed. Fig. Figure 9B is a cross-sectional view illustrating the autosampler when the holding section opening and closing mechanism is closed. Fig. 9A and Fig. 9B correspond to a cross-sectional view along the line CC of Fig. 6. Fig. 9A and Fig. 9B show the state of placing the sample plate assembly 28 on the stage 34. The sample mounting section 27 is in the Fig. 9A and Fig. 9B not shown.

[0123] As in Fig. 9A and Fig. As illustrated in Fig. 9B, an X-axis slider 71 supports the stage base 42. The X-axis slider 71 is placed on the X-axis guide rail 44 provided on the X-axis drive base 43 so as to be bidirectionally movable along the X-axis direction.

[0124] The cam member 72 is fixed to the X-axis drive base 43. The upper surface of the cam member 72 includes the inclined cam surface 73 configured to raise and lower the cam roller 60. The cam member 72 is positioned parallel to the X-axis guide rail 44, so that the inclination direction of the inclined cam surface 73 is parallel to the X-axis guide rail 44. The cam member 72 can be provided as long as it is parallel to a partial zone of the X-axis guide rail 44.

[0125] As in Fig. As illustrated in Fig. 9A, in a region where the cam member 72 is not inserted, the cam roller 60 descends without contacting the inclined cam surface 73. This state releases the pressing portions 61L and 61R from pressing the pressing protrusion portions 70L and 70R. The tension spring 69 exerts a force in the tension direction to direct the outer sides of the holding portions 51L and 51R, where the holding nails 67L and 67R are formed, upward. The holding nails 67L and 67R are inserted into the holding nail engagement holes 33L and 33R of the sample adapter 29 from the inside to the outside, thereby entering the engagement state.

[0126] In the engaged state, the retaining nails 67L and 67R prevent the sample plate assembly 28 from being displaced upward. Even if an attempt is made to lower the sample plate assembly 28 by removing it from the cathode end 11 of the capillary 6, the retaining nails 67L and 67R exert a downward force to fix the sample plate assembly 28 to the stage 34. Consequently, the sample plate assembly 28 can be prevented from lifting.

[0127] In a zone in which the cam element 72 is inserted, as in Fig. As illustrated in Figure 9B, the cam roller 60 contacts the inclined cam surface 73 and rises. In this state, the pressing portions 61L and 61R press the pressing protrusion portions 70L and 70R. The outer sides of the holding portions 51L and 51R, which form the holding nails 67L and 67R, incline and are directed inward against the force applied by the tension spring 69. The holding nails 67L and 67R are disengaged from the holding nail engagement holes 33L and 33R and are not inserted into them, entering the disengaged state.

[0128] In the released state, the holding nails 67L and 67R do not restrict the upward displacement of the sample plate assembly 28. The holding nails 67L and 67R move inward from the holding nail engagement holes 33L and 33R and retract to such a position as to create a gap against the side surface of the sample adapter 29. In this state, the sample plate assembly 28 placed on the stage 34 can be freely fastened and detached in the vertical direction.

[0129] As in Fig. 9A and Fig. As illustrated in Fig. 9B, the holding portions 51L and 51R extend upwardly within the outer edge of the sample adapter 29 as viewed from directly below it, pass through the open bottom surface of the sample adapter 29, and reach into the holding nail engagement holes 33L and 33R. The holding nails 67L and 67R can switch between the engaged state of being inserted into the holding nail engagement holes 33L and 33R from the inside to the outside and the released state of being released from the holding nail engagement holes 33L and 33R.

[0130] According to a plan view, this structure allows the holding portions 51L and 51R to operate within the outer edge of the sample adapter 29. The engaged state maximizes the width in the horizontal direction, making it difficult to protrude outward in the horizontal direction. It is not necessary to provide space for the holding portion opening and closing mechanism 47 around the sample plate assembly 28. It is possible to reduce the area occupied by the container fixing mechanism and save space within the electrophoresis apparatus 1.

[0131] Fig. 10A and Fig. 10B are diagrams illustrating the process of transferring the sample plate assembly to a stage. Fig. 10A and Fig. 10B illustrates the circumference of the sample mounting portion 27 as viewed from the Y-axis direction. Fig. 10A illustrates the state before the sample plate assembly 28 mounted on the sample mounting section 27 is transferred to the stage 34. Fig. 10B illustrates the state after the sample plate assembly 28 mounted on the sample mounting section 27 is transferred to the stage 34.

[0132] As in Fig. 10A and Fig. As illustrated in Figure 10B, the cam member 72 may be provided at one end of the X-axis drive base 43 near the sample mounting portion 27. The cam member 72 may be fixed to the side surface or upper surface of the X-axis drive base 43. The cam member 72 is provided at a height that contacts the depressed cam roller 60.

[0133] In Fig. 10A and Fig. 10B, the cam member 72 is shaped approximately in a trapezoid as seen from the side and is provided in a rectangular parallelepiped shape with one side inclined along the longitudinal direction. The cam member 72 includes an obliquely provided inclined cam surface 73 and a horizontally provided flat cam surface 74 on the upper surface. The cam member 72 is installed such that the inclination direction of the inclined cam surface 73 is parallel to the movement path of the stage 34 in the X-axis direction.

[0134] The inclined cam surface 73 is provided on the rear upper surface of the cam member 72 and is positioned toward the rear of the electrophoresis device 1. The inclined cam surface 73 inclines to increase the height from the rear to the front. The inclined cam surface 73 acts as a drive that displaces the cam roller 60 in the vertical direction.

[0135] The flat cam surface 74 is provided on the front upper surface of the cam member 72 and is positioned toward the front of the electrophoresis device 1. The flat cam surface 74 is provided as a substantially horizontal plane. The flat cam surface 74 allows the stage 34 to move without displacing the cam roller 60 substantially in the vertical direction. The flat cam surface 74 can ensure a zone for maintaining the released state, making it possible to eliminate the need for precise alignment of the sample plate assembly 28.

[0136] As in Fig. 10A and Fig. As illustrated in Figure 10B, while the cam member 72 is shaped approximately in a trapezoid, the inclined cam surface 73 and the flat cam surface 74 may be connected via a curved surface. The inclined cam surface 73 may be provided at an appropriate inclination angle according to the design of the holding portion opening and closing mechanism 47. The cam member 72 may be appropriately provided in terms of height, width, length, and an aspect ratio between the inclined cam surface 73 and the flat cam surface 74 according to the design of the holding portion opening and closing mechanism 47.

[0137] As in Fig. As illustrated in Figure 10A, before the sample plate assembly 28 mounted on the sample mounting section 27 is transferred to the stage 34, the sliding section 50 is controlled so that the stage 34 is positioned lower than the sample mounting section 27. The sliding section 50 is then moved in the X-axis direction to be positioned below the sample mounting section 27. Then, the X-axis drive base 43 is moved in the Z-axis direction to raise the sliding section 50.

[0138] The cam roller 60 contacts the cam member 72 while the sliding portion 50 moves in the X-axis direction. The cam roller 60 rises along the inclined cam surface 73 from an E2 position, the lower end of the inclined cam surface 73, to an E1 position, the upper end of the inclined cam surface 73. Therefore, between the E2 position and the E1 position, the state of the holding portion opening and closing mechanism 47 gradually changes to display intermediate values ​​corresponding to the height of the cam roller 60. The holding portion opening and closing mechanism 47 gradually changes the states of the holding portions 51L and 51R and the holding nails 67L and 67R while the stage 34 moves between the E2 position and the E1 position.

[0139] When the cam roller 60 passes through the E1 position, the holding pins 67L and 67R detach from the holding pin engagement holes 33L and 33R of the sample adapter 29 and enter the released state. At and after the E1 position, the flat cam surface 74 regulates the height of the cam roller 60 to be approximately constant. At and after the E1 position, an approximately constant released state is maintained to prevent the holding pins 67L and 67R from being displaced.

[0140] Even after the holding nails 67L and 67R enter the released state, the sliding portion 50 continues to move in the X-axis direction and stops below the sample mounting portion 27. The sliding portion 50 stops at a position where the X-axis positions of the holding nails 67L and 67R coincide with the X-axis positions of the holding nail engagement holes 33L and 33R. Then, the sliding portion 50 moves upward to transfer the sample plate assembly 28 to the stage 34.

[0141] According to this operation, the holding nails 67L and 67R enter the released state when the sample plate assembly 28 is transferred to the stage 34. It is possible to avoid interference between the holding nails 67L and 67R and the sample plate assembly 28. The holding nails 67L and 67R enter the released state at the E1 position before the stop position. It is possible to avoid interference between the holding nails 67L and 67R and the sample plate assembly 28, even if the sample plate assembly 28 is misaligned in the X-axis direction. It is possible to omit precise alignment of the sample plate assembly 28 relative to the sample mounting section 27. The state of the holding section opening and closing mechanism 47 changes gradually, causing no sudden movements. It is possible to standardize the condition of the retaining nails 67L and 67R over a long zone.

[0142] As in Fig. 10B, after the sample plate assembly 28 mounted on the sample mounting section 27 is transferred to the stage 34, the sliding section 50 is moved in the X-axis direction toward the other end of the X-axis movement path of the stage 34 where the capillary assembly 2 is mounted.

[0143] As the sliding portion 50 retreats toward the rear of the sample mounting portion 27, the cam roller 60 moves along the flat cam surface 74 and then descends along the inclined cam surface 73 from the E1 position as the upper end of the inclined cam surface 73 to the E2 position as the lower end of the inclined cam surface 73. Between the E1 position and the E2 position, the state of the holding portion opening and closing mechanism 47 gradually changes to display intermediate values ​​corresponding to the height of the cam roller 60. The holding portion opening and closing mechanism 47 gradually changes the states of the holding portions 51L and 51R and the holding nails 67L and 67R as the stage 34 moves between the E2 position and the E1 position. The cam roller 60 descends by the height H of the inclined cam surface 73.

[0144] When the cam roller 60 passes through the E2 position, the holding pins 67L and 67R enter the holding pin engagement holes 33L and 33R of the sample adapter 29 and enter the engaged state. At and after the E2 position, the cam roller 60 maintains an approximately constant height because it is released from the cam member 72. At and after the E2 position, an approximately constant engagement state is maintained to prevent the holding pins 67L and 67R from being displaced.

[0145] Even after the holding nails 67L and 67R enter the engaged state, the sliding portion 50 continues to move in the X-axis direction and stops below the sample mounting portion 27. Then, the sliding portion 50 moves upward to insert the capillary 6 into the recess of the sample plate 30 of the sample plate assembly 28 and aspirate the sample. After electrophoresis is completed, the sliding portion 50 moves downward to remove the capillary 6 from the sample plate assembly 28 while maintaining the engaged state.

[0146] According to this operation, the holding nails 67L and 67R enter the engaged state when the capillary 6 is removed from the sample plate assembly 28. It is possible to prevent the sample plate assembly 28 from lifting due to the frictional force between the side surface of the capillary 6 and the inner surface of the opening of the septum 31. The holding nails 67L and 67R enter the engaged state at the E2 position before the stop position. It is possible to fix the sample plate assembly 28 while the sample plate assembly 28 is being transported in the X-axis direction and the capillary 6 is being inserted into the sample plate assembly 28. The state of the holding section opening and closing mechanism 47 changes gradually, which does not cause sudden movements. It is possible to uniform the state of the holding nails 67L and 67R over a long zone.

[0147] In the electrophoresis device 1, the sliding section 50 includes the stage 34, which provides a transfer area and a connection area at both ends along the X-axis movement path. The transfer area, located at one end in the X-axis direction, enables the transfer of the sample plate assembly 28 onto the stage 34. The connection area, located at the other end in the X-axis direction, enables the capillary 6 to connect to and disconnect from the sample plate assembly 28. The holding section opening and closing mechanism 47 can operate along such a movement path in conjunction with the movement of the stage 34.

[0148] The X-axis trajectory of the stage 34 includes the E1 position and the E2 position between one side where the transfer area is located and the other side where the connection area is located. The E1 position corresponds to the upper end of the inclined cam surface 73. The E2 position corresponds to the lower end thereof. The E1 position serves as the first position where the transition to the disengaged state ends and the transition to the engaged state begins. The E2 position serves as the first position where the transition to the engaged state ends and the transition to the engaged state begins.

[0149] The first and second positions may be provided in a predetermined order at appropriate locations on the X-axis movement path of the stage 34. The cam member 72 may be provided at an appropriate position on the X-axis drive base 43 so that the inclined cam surface 73 and the flat cam surface 74 ensure appropriate lengths. However, from the perspective of fixing and stably supporting the moving sample plate assembly 28, the first and second positions are preferably positioned toward the transfer area, which is the center of the X-axis movement path of the stage 34.

[0150] Fig. 11 and Fig. 12 are diagrams illustrating the operation of the mechanism for opening and closing the holding section equipped with a cam roller. Fig. 11 and Fig. 12 illustrate the scope of the mechanism 47 for opening and closing the holding section supporting the stage 34, viewed from the Y-axis direction. The time-based process of transporting the stage 34 follows (a), (b), and (c) sequentially in Fig. 11 and (d), (e) and (f) successively in Fig. 12.

[0151] The sample plate assembly 28 is mounted on the sample mounting section 27 and then transferred to the stage 34. Before the sample plate assembly 28 is transferred, the stage 34 is transported backward from the sample mounting section 27 by driving the X-axis driving section 35, the Z-axis driving section 36, and the Y-axis driving section 37.

[0152] Before the sample plate assembly 28 is transferred, the stage 34 is controlled to a height lower than the lower surface of the sample plate assembly 28 mounted on the sample mounting section 27. The stage 34 is then transported below the sample mounting section 27 by the drive of the X-axis drive section 35.

[0153] As in Fig. As illustrated in Figure 11(a), the stage 34 is removed from the sample mounting section 27. In this state, the cam roller 60 is positioned behind the cam member 72. The cam roller 60 does not contact the cam member 72. At this moment, the holding pins 67L and 67R remain in an engaged state.

[0154] As in Fig. As illustrated in Figure 11(b), the stage 34 approaches the sample mounting section 27. The cam roller 60 contacts the cam member 72 and begins to rise along the inclined cam surface 73. At this moment, the holding nails 67L and 67R begin to transition from the engaged state to the disengaged state.

[0155] As in Fig. As illustrated in Figure 11(c), the stage 34 further approaches the sample mounting portion 27. The cam roller 60 stops rising along the inclined cam surface 73. At this moment, the holding nails 67L and 67R stop transitioning from the engaged state to the disengaged state and enter the disengaged state.

[0156] As in Fig. As illustrated in Figure 12(d), the stage 34 reaches immediately below the sample mounting section 27. The X-axis positions of the holding nails 67L and 67R are controlled to coincide with the X-axis positions of the holding nail engagement holes 33L and 33R of the sample plate assembly 28 mounted on the sample mounting section 27. The positioning pin 49 on the top surface of the stage 34 and the positioning hole in the bottom surface of the sample adapter 29 are approximately concentrically positioned. At this moment, the holding nails 67L and 67R remain in a released state.

[0157] As in Fig. As illustrated in Figure 12(e), the stage 34 rises from below the sample mounting section 27. The slotted portion 40 of the sample mounting section 27 is open at the bottom. The sample plate assembly 28 mounted on the sample mounting section 27 is transferred as it is lifted up. The positioning pin 49 on the top of the stage 34 fits into the positioning hole in the bottom of the sample adapter 29. Therefore, the sample plate assembly 28 is positioned at a predetermined position on the stage 34. At this time, the holding nails 67L and 67R remain in a released state.

[0158] As in Fig. As illustrated in Figure 12(f), the X-axis drive section 35 drives and transports the stage 34 to the rear of the sample mounting section 27. The stage 34 moves from above the sample mounting section 27 to above the X-axis drive base 43 and is then transported to the electrophoresis connection area. As the stage 34 is transported to the rear of the sample mounting section 27, the cam roller 60 descends along the inclined cam surface 73 to its original height. The holding pins 67L and 67R transition from the disengaged state to the engaged state.

[0159] In Fig. 12 (f) and Fig. 3, dash-dotted lines represent the structure and process around the sample plate assembly 28 in the connection area. As in Fig. As shown in Figure 12(f), the cathode end 11 of the capillary 6 protrudes vertically downward. Consequently, the stage 34 is transported below the cathode end 11 of the capillary 6 by the drive of the X-axis drive section 35 and the Y-axis drive section 37, and then transported upward by the drive of the Z-axis drive section 36.

[0160] In the connection area, the stage 34 rises toward the cathode end 11 of the capillary 6, whose relative position is fixed. The capillary 6 thereby penetrates the septum 31 and is inserted into the recess of the sample plate 30. The stage 34 rises upward while the holding pins 67L and 67R remain engaged. The capillary 6 is inserted into the sample plate assembly 28. After the electrophoresis ends, the stage 34 moves downward to remove the sample plate assembly 28 from under the capillary 6. The stage 34 moves downward while the holding pins 67L and 67R remain engaged. The capillary 6 remains away from the sample plate assembly 28.

[0161] When the capillary 6 is pulled out of the opening of the septum 31, a frictional force is generated between the side of the capillary 6 and the inner surface of the opening of the septum 31. The sample plate assembly 28 may become detached, resulting in insufficient force to remove the sample plate assembly 28 downward. In such a case, the sample plate assembly 28 may lift off the stage 34 due to the frictional force and remain inserted into the cathode end 11.

[0162] However, the holding nails 67L and 67R enter the engaged state while the stage 34 moves and remain laterally inserted into the holding nail engagement holes 33L and 33R provided in the side surface of the sample adapter 29. The stage 34 is driven to move downward, so that the holding nails 67L and 67R generate a downward force greater than the frictional force generated between the capillary 6 and the septum 31.

[0163] The holding nails 67L and 67R remain in the engaged state, exerting a downward force on the sample plate assembly 28 and generating a large force to remove the sample plate assembly 28 downward. The sample plate assembly 28 can be effectively prevented from lifting off the stage 34.

[0164] Fig. Figure 13 is a diagram illustrating the connection positions of the sample plate assembly connected with capillaries. Fig. Figure 13 illustrates the sample plate assembly 28, viewed in the Z-axis direction. Reference numerals 75 to 78 represent the respective connection positions. As shown in Fig. As illustrated in Figure 13, the sample plate assembly 28 includes the connection positions. Multiple connection positions may be provided.

[0165] The connection position indicates the wells in the sample plate 30 where the capillaries 6 forming the capillary array 2 are simultaneously inserted or removed. The number of connection positions depends on the number of wells in the sample plate 30 and the number of capillaries 6 forming the capillary array 2.

[0166] In Fig. 13, for example, the sample plate 30 includes 12 rows of wells along the X-axis direction and 8 rows of wells along the Y-axis direction, resulting in a total of 96 wells. Assume that the capillary array 2 is formed of 3 rows of capillaries 6 along the X-axis direction and 8 rows of capillaries 6 along the Y-axis direction. In this case, the number of connection positions is 4, as indicated by reference numerals 75 to 78. The following description defines reference numeral 75 as the K position, 76 as the L position, 77 as the M position, and 78 as the N position.

[0167] Each connection position requires alignment with the cathode ends 11 for the capillaries 6 that form the capillary array 2. The Fig. The configuration illustrated in Figure 13 requires the sample prepared in the wells to be sucked while sequentially aligning it with the K position, the L position, the M position, and the N position. It also requires the sample plate assembly 28 to be aligned at least in the X-axis direction.

[0168] Fig. Figure 14 illustrates the relationship between the stage positions in the X-axis direction and the open / closed state of the holding section. Fig. 14, the horizontal axis indicates the positions of the stage 34 in the X-axis direction. The vertical axis indicates the opening degree of the holding sections 51L and 51R according to the movement of the holding nails 67L and 67R. The left end of the horizontal axis indicates the transfer area. The right end of the horizontal axis indicates the connection area. Fig. 14 the K position is aligned with the connection area.

[0169] As in Fig. As illustrated in Figure 14, the opening degree of the holding sections 51L and 51R switches between the open state and the closed state in association with the movement of the stage 34 in the X-axis direction. The approximately constant open state is maintained toward the transfer area in the X-axis direction. On the other hand, the approximately constant closed state is maintained toward the connection area in the X-axis direction. The opening degree varies gradually while remaining in an intermediate state between the first and second positions located between the transfer area and the connection area.

[0170] The first position corresponds to the E1 position, which is the upper end of the inclined cam surface 73. The first position completes the transition of the holding sections 51L and 51R to the open state and begins the transition of the holding sections 51L and 51R to the closed state. Toward the transfer area from the first position, the holding nails 67L and 67R remain released to release the sample plate assembly 28 from the stage 34.

[0171] The second position corresponds to the E2 position, which is the lower end of the inclined cam surface 73. The second position completes the transition of the holding sections 51L and 51R to the closed state and begins the transition of the holding sections 51L and 51R to the open state. Toward the connection area from the second position, the holding nails 67L and 67R enter the engaged state to fix the sample plate assembly 28 to the stage 34.

[0172] As in Fig. As illustrated in Figure 14, when multiple connection positions are specified, the second position is preferably located toward the transfer area away from all connection positions. This configuration can allow the holding nails 67L and 67R to maintain the engaged state closer toward the connection area from the second position. The sample plate assembly 28 can be reliably fixed to the stage 34 at all connection positions.

[0173] Fig. 15A is a diagram illustrating an exemplary shape of the holding portion. Fig. 15B is an enlarged view illustrating a relevant part of the exemplary shape of the holding portion. Fig. 15A and Fig. 15B are partial cross-sectional views of the holding portion 51R, viewed from the X-axis direction, engaging the holding portion 67R with the right holding nail engaging hole 33R. In Fig. In FIG. 15A, a solid line represents the engaged state of the holding portion 67R, and a dashed line represents the released state of the holding portion 67R.

[0174] As in Fig. 15A and Fig. 15B, the right holding portion 51R may include a holding nail 67R and a bent portion 80. The holding nail 67R may extend horizontally by rotating around the second support shaft 65R. The bent portion 80 is provided at the tip of the holding nail 67R and protrudes in a direction perpendicular to the holding nail 67R. Similarly, the left holding portion 51L may include a holding nail 67L and the bent portion 80. The holding nail 67L may extend horizontally by rotating around the second support shaft 65L. The bent portion 80 is provided at the tip of the holding nail 67L and protrudes in a direction perpendicular to the holding nail 67L.

[0175] It is preferable that the sample plate assembly 28 mounted on the stage 34 hardly slips from the stage 34 even when the capillary 6 is not inserted or removed. The bent portion 80 provided at the tip of the holding nails 67L and 67R can prevent the engaged holding nails 67L and 67R from being disengaged even when a large unintentional upward force is applied to the holding nails 67L and 67R.

[0176] When the retaining nails 67L and 67R are inserted into the retaining nail engagement holes 33L and 33R to enter the engagement state, the bent portion 80 is positioned outside the retaining nail engagement holes 33L and 33R. The bent portion 80 may be shaped to include an inclined surface 79. When the retaining nails 67L and 67R are inserted into the retaining nail engagement holes 33L and 33R, the inclined surface 79 is positioned to face the lower inner wall of the retaining nail engagement holes 33L and 33R. The inclined surface 79 is shaped such that the protrusion width perpendicular to the retaining nails 67L and 67R increases from the base to the tip of the retaining nails 67L and 67R.

[0177] As in Fig. 15B, the inclined surface 79 may be provided to ensure a predetermined angle θ between the inclined surface 79 and the base end of the holding nails 67L and 67R in contact with the lower inner wall of the holding nail engagement holes 33L and 33R. The angle θ between the inclined surface 79 and the base end of the holding nails 67L and 67R is advantageously formed to generate a predetermined torque that prevents the engagement state from being released.

[0178] For example, assume that an upward force F acts on the sample plate assembly 28. In such a case, if the bent portion 80 is not provided, the holding nails 67L and 67R rotate around the second support shafts 65L and 65R due to the upward force F to move the tip side inward and will slip from the holding nail engagement holes 33L and 33R. Such slippage is noticeable in the holding portions 51L and 51R made of resin.

[0179] When the inclined surface 79 is provided with a suitable angle θ, the retaining nails 67L and 67R will slide from the retaining nail engagement holes 33L and 33R, allowing the inclined surface 79 to contact the outer corner of the inner wall of the retaining nail engagement holes 33L and 33R, thereby generating a component force Fcosθ in a direction perpendicular to the inclined surface 79. Therefore, a torque M = R × Fcosθ can be generated with respect to the distance R between the bent portion 80 and the center of the second support shafts 65L and 65R.

[0180] The bent portion 80 allows the angle θ of the inclined surface 79 and the distance R between the bent portion 80 and the rotation center to be appropriately adjusted. The retaining nails 67L and 67R will slide from the retaining nail engagement holes 33L and 33R and contact the inner wall or periphery of the retaining nail engagement holes 33L and 33R, thereby generating a reaction force in the direction along which the retaining nails 67L and 67R are inserted into the retaining nail engagement holes 33L and 33R. It is possible to make it difficult for the retaining nails 67L and 67R to be accidentally released.

[0181] Fig. Figure 16 is a perspective view illustrating the placement of the autosampler and sample mounting section. Fig. 16 shows a partially sectional view of an example structure of the autosampler 25 and the sample mounting section 27 included in the electrophoresis apparatus 1.

[0182] As in Fig. As illustrated in Figure 16, the sample mounting section 27 can mount multiple sample plate assemblies 28 in parallel. The autosampler 25 transports the stage 34 in the Y-axis direction to transfer each sample plate assembly 28.

[0183] As in Fig. 9A and Fig. 9B, the holding portions 51L and 51R extend, as viewed from directly below and inwardly of the outer edge of the sample plate assembly 28, pass through the opened bottom surface of the sample adapter 29, and reach into the holding nail engagement holes 33L and 33R.

[0184] The holding nails 67L and 67R provided at the tip of the holding portions 51L and 51R can switch between the engaged state inserted into the holding nail engaging holes 33L and 33R from the inside to the outside and the released state removed from the holding nail engaging holes 33L and 33R to the inside.

[0185] According to a plan view, the holding portions 51L and 51R are positioned within the outer edge of the sample plate assembly 28 and can operate within the outer edge of the sample plate assembly 28. The holding nails 67L and 67R are displaced within the outer edge of the sample plate assembly 28 and do not protrude outward from their positions in the engaged state.

[0186] Therefore, as in Fig. As illustrated in FIG. 16, it is possible to avoid interference between the sample plate assembly 28 and the holding portions 51L and 51R when the sample mounting portion 27 mounts a plurality of sample plate assemblies 28, even if the holding portions 51L and 51R rise from below. A distance between the slit portions 40 can be reduced, making it possible to reduce a distance between the sample plate assemblies 28 mounted on the sample mounting portion 27. <Zusammenfassung der ersten Ausführungsform>

[0187] As described above, the electrophoresis apparatus 1 according to the first embodiment includes the sample plate assembly 28 as a container for containing a sample or a reagent, the stage 34 for mounting the sample plate assembly 28, and the autosampler 25 for moving the stage 34 at least in the horizontal direction. The electrophoresis apparatus 1 also includes the holding portions 51L and 51R as engaging members for switching between an engaged state in which the sample plate assembly 28 is engaged with the stage 34 and a disengaged state in which the sample plate assembly 28 is disengaged from the stage 34, and the holding portion opening / closing mechanism 47 as a switching mechanism for switching the holding portions 51L and 51R between the engaged state and the disengaged state.

[0188] The holding section opening and closing mechanism 47 switches the states of the holding sections 51L and 51R in conjunction with the movement of the stage 34 between the first and second positions located between the transfer area and the connection area. When the stage 34 is positioned on one side of the X-axis movement path, the holding sections 51L and 51R enter the released state. When the stage 34 is positioned on the other side of the X-axis movement path, the holding sections 51L and 51R enter the engaged state.

[0189] The stage 34 moves horizontally between one side of the X-axis movement path and the first position while the holding portions 51L and 51R remain in the released state. The stage 34 moves horizontally between the other side of the X-axis movement path and the second position while the holding portions 51L and 51R remain in the engaged state.

[0190] When the capillary 6 is removed from the opening of the septum 31 in the connection area, the holding sections 51L and 51R fix the sample plate assembly 28 on the stage 34, thereby preventing the sample plate assembly 28 from lifting from the stage 34. The electrophoresis device 1 can ensure high robustness against the removal of the capillary 6. The transfer area ensures the detached state. The sample plate assembly 28 can be transferred appropriately while avoiding, for example, interference between the sample plate assembly 28 and the holding sections 51L and 51R.

[0191] The holding section opening and closing mechanism 47 operates in conjunction with the movement of the stage 34, eliminating the need for an additional power source to drive the holding sections 51L and 51R. A small and simple structure can achieve operations associated with the movement of the stage 34. Space can be saved and costs can be reduced without the need for a drive source such as an electric actuator and wiring, or a control circuit for the drive source. The electrophoresis device 1 is available based on a small and simple structure.

[0192] The stage 34 moves horizontally while maintaining the released state or the engaged state on both sides of the horizontal movement path. The opening degree of the holding sections 51L and 51R does not change significantly, thereby maintaining the holding nails 67L and 67R in a roughly constant state. Unlike Patent Literatures 1 to 3, it is possible to reduce the effects of positional errors or displacement errors in the sample plate assembly 28. The connection section can ensure the attached state of the sample plate assembly 28. The transfer section eliminates the need for precise alignment for manufacturing the sample plate assembly 28. The holding sections 51L and 51R operate at a low speed. It is possible to reliably ensure the displacement of the holding nails 67L and 67R with a small force and reduce the impact on the surroundings.It is possible to provide the electrophoresis apparatus 1 with reduced errors and high reliability.

[0193] In the electrophoresis apparatus 1 according to the first embodiment, the holding sections 51L and 51R are symmetrically positioned and symmetrically driven. The sample plate assembly 28 can be stably mounted structurally or mechanically without imbalance.

[0194] The holding nails 67L and 67R can extend horizontally by rotating around the second support shafts 65L and 65R to prevent the sample plate assembly 28 mounted on the stage 34 from being lifted upward due to the frictional force between the capillary 6 and the sample plate assembly 28. The holding nails 67L and 67R enter the engaged state by being inserted from the inside outward, and enter the released state by being removed from the outside inward from the holding nail engagement holes 33L and 33R provided on the side surfaces of the sample adapter 29.

[0195] As viewed from a top view, the holding portions 51L and 51R are provided to extend upward from directly below the sample adapter 29 within its outer edge, pass through the open bottom surface of the sample adapter 29, and reach into the holding pin engagement holes 33L and 33R. The holding portions 51L and 51R are moved by the second support shafts 65L and 65R positioned within the holding pin engagement holes 33L and 33R.

[0196] According to a plan view, the holding sections 51L and 51R and the holding nails 67L and 67R move inward, thereby narrowing the working area. It is possible to avoid interference between the sample plate assemblies 28 and the holding sections 51L and 51R even when multiple sample plate assemblies 28 are placed in parallel in the transfer area. It is possible to reduce the distance between the sample plate assemblies 28 mounted on the sample mounting section 27 and to reduce the overall width of the sample mounting section 27. The electrophoresis apparatus 1 can be downsized.

[0197] The holding nails 67L and 67R are provided to be able to extend horizontally by rotation around the second support shafts 65L and 65R, and each include the bottom surface contacting the lower inner walls of the holding nail engagement holes 33L and 33R. Unlike Patent Literature 1, it is possible to reliably exert at least a downward force on the sample plate assembly 28 when the capillary 6 inserted into the sample plate assembly 28 is removed. The sample plate assembly 28 can be effectively prevented from lifting.

[0198] The holding nails 67L and 67R may include the bent portion 80. When the holding nails 67L and 67R slip from the holding nail engagement holes 33L and 33R, it is possible to generate a reaction force in the direction along which the holding nails 67L and 67R are inserted into the holding nail engagement holes 33L and 33R. The holding nails 67L and 67R in the engaged state are hardly accidentally released, thereby reliably fixing the sample plate assembly 28. The highly reliable electrophoresis device 1 can be provided to prevent the sample plate assembly 28 from lifting or slipping even when a large force is applied to remove the sample plate assembly 28 or an accidental force is applied.

[0199] In the electrophoresis apparatus 1 according to the first embodiment, the switching mechanism uses the cam member 72, which has the inclined cam surface 73 formed along the moving path of the stage 34, and the holding section opening and closing mechanism 47, which includes the holding sections 51L and 51R and the cam roller 60. The mechanical mechanism can operate the holding sections 51L and 51R without using a driven actuator or power source. It is possible to prevent the sample plate assembly 28 from lifting based on a simple structure, which reduces costs and is suitable for downsizing.

[0200] In the electrophoresis device 1 according to the first embodiment, the sample adapter 29, which is fixed by the holding nails 67L and 67R, has holding nail engagement holes 33L and 33R for inserting the holding nails 67L and 67R. It is possible to eliminate the need to provide a flange or groove on the container to be fixed, as described in Patent Literature 3. It is possible to ensure the compatibility of the sample plate assembly 28, which serves, for example, as a container for samples.

[0201] The above description explains the example of the sample plate assembly 28 as a container to be lifted. The container to be lifted can be represented, for example, according to the configurations other than the cathode-side buffer container 13 or the sample plate 30. Lifting prevention of the cathode-side buffer container 13 can be applied to cases where analysis is performed while switching between multiple cathode-side buffer containers 13. Similar to the sample plate assembly 28, the cathode-side buffer container 13 can also be transported by the autosampler 25 instead of the buffer transport section 26. <Zweite Ausführungsform>

[0202] According to the second embodiment, the electrophoresis apparatus 1 uses the engaging element represented by the holding portions 51L and 51R, which open and close while rotating around the central axis parallel to the horizontal direction. The switching mechanism for switching the states of the engaging element uses a magnetic mechanism that operates by magnetic force in conjunction with the movement of the stage 34. The above-described cam mechanism is replaced by a pair of magnetic elements for operation. One of the magnetic elements on the driven side replaces the cam roller 60 and is provided for the holding portion opening and closing mechanism 47, which moves with the stage 34. The other of the magnetic elements on the driving side replaces the cam element 72 and is provided on the moving path of the stage 34 in the X-axis direction.

[0203] Fig. 17 is an exploded perspective view of the mechanism for opening and closing the holding section equipped with a magnetic element. Fig. Figure 18 is a diagram illustrating a magnetic element and magnetic poles acting as a driven side. Fig. Figure 19 is a diagram illustrating a magnetic element and magnetic poles acting as a driving side. Fig. Fig. 17 is an exploded perspective view of the holding portion opening and closing mechanism 47, which includes the holding portions 51L and 51R and the magnetic members.

[0204] As in Fig. As illustrated in Figure 17, the holding portion opening and closing mechanism 47 includes a pair of left and right holding portions 51L and 51R as the engaging elements that secure the sample plate assembly 28. According to the second embodiment, the holding portion opening and closing mechanism 47 includes a first magnet 84, a magnetic element, on the opposite side of the holding portions 51L and 51R, instead of the cam roller 60.

[0205] As in Fig. 18, the first magnet 84 is structured to include two types of magnetic poles 93 and 94 along an axial direction, so that one surface corresponds to a single magnetic pole. In Fig. 18, the first magnet 84 is vertically polarized. The upper magnetic pole 93 corresponds to the N pole. The lower magnetic pole 94 corresponds to the S pole. The first magnet 84 is positioned so that the two magnetic poles 93 and 94 are aligned along a direction perpendicular to the lower surface of the oscillating element 56.

[0206] The first magnet 84 is attached to the lower surface of the vibrating element 56, behind the second support shaft hole 57 of the vibrating element 56. The first magnet 84 is installed at a location where it directly overlies a second magnet 85. Any shape and any number of first magnets 84 can be installed on the lower surface of the vibrating element 56.

[0207] As in Fig. As illustrated in Figure 19, the second magnet 85 is shaped approximately like a trapezoid when viewed from the side, with one side inclined along the longitudinal direction. The second magnet 85 is structured to include two types of magnetic poles 93 and 94 vertically, so that the inclined surface provides a single magnetic pole. Fig. 19, the second magnet 85 includes the upper magnetic pole 93 as the S-pole and the lower magnetic pole 94 as the N-pole.

[0208] The second magnet 85 is fixed to one end of the X-axis drive base 43 near the slot portion 40 of the sample mounting portion 27. The second magnet 85 is installed so that the tilt direction is parallel to the X-axis movement path of the stage 34 formed by the X-axis guide rail 44.

[0209] The first magnet 84 and the second magnet 85 are positioned so that their magnetic poles of the same polarity face each other. When the first magnet 84 or the second magnet 85 is used, the oscillating element 56 and the X-axis drive base 43 are preferably made of a non-magnetic material to avoid the influence of unintended magnetic forces. Non-magnetic materials include, for example, brass, an aluminum alloy, austenitic stainless steel, ceramic, and resin.

[0210] The use of the first magnet 84 and the second magnet 85 generates a mutually repulsive magnetic force to drive and lift the back of the oscillating member 56. The second magnet 85 includes a single inclined magnetic pole and can gradually lift the back of the oscillating member 56 in conjunction with the movement of the stage 34. Therefore, the states of the holding sections 51L and 51R are gradually changed.

[0211] Fig. 20 is a diagram illustrating the operation of the mechanism for opening and closing the holding section equipped with the magnetic element. Fig. Figure 20 illustrates the scope of the mechanism 47 for opening and closing the holding section supporting the stage 34, viewed from the Y-axis direction. The time-based process of transporting the stage 34 follows (a), (b), and (c) sequentially in Fig. 20.

[0212] The sample plate assembly 28 is mounted on the sample mounting section 27 and then transferred to the stage 34. The stage 34 is controlled to be lower than the lower surface of the sample plate assembly 28 mounted on the sample mounting section 27 and then transported downward from the sample mounting section 27 by the drive of the X-axis drive section 35.

[0213] As in (a) of Fig. As illustrated in Figure 20, the stage 34 is removed from the sample mounting section 27. In this state, the first magnet 84 is positioned behind the second magnet 85. The first magnet 84 is not repelled by the magnetic force of the second magnet 85. At this moment, the holding nails 67L and 67R remain in an engaged state.

[0214] As in (b) of Fig. As illustrated in Figure 20, the stage 34 approaches the sample mounting section 27. The first magnet 84 is repelled by the magnetic interaction with the second magnet 85 and begins to rise along the second magnet 85. At this moment, the holding nails 67L and 67R begin to transition from the engaged state to the released state.

[0215] As in (c) of Fig. As illustrated in Figure 20, the stage 34 continues to approach the sample mounting section 27. The first magnet 84 stops rising along the second magnet 85. At this moment, the holding nails 67L and 67R stop transitioning from the engaged state to the disengaged state and enter the disengaged state.

[0216] Then, the stage 34 rises and falls relative to the sample mounting section 27. The sample plate assembly 28 mounted on the sample mounting section 27 is transferred to the stage 34. Then, the stage 34 is transported to the rear of the sample mounting section 27. The first magnet 84 descends to its original height while interacting with the second magnet 85 due to the magnetic force. The holding nails 67L and 67R transition from the released state to the engaged state. <Zusammenfassung der zweiten Ausführungsform>

[0217] As above, the electrophoresis apparatus 1 according to the second embodiment, similar to the electrophoresis apparatus 1 according to the first embodiment, includes the sample plate assembly 28, the stage 34, and the autosampler 25. It includes the holding portions 51L and 51R and the holding portion opening and closing mechanism 47.

[0218] Therefore, the electrophoresis apparatus 1 according to the second embodiment can achieve the same effect as the electrophoresis apparatus 1 according to the first embodiment based on the holding portions 51L and 51R and the holding portion opening and closing mechanism 47.

[0219] In the electrophoresis apparatus 1 according to the second embodiment, the switching mechanism uses the holding section opening and closing mechanism 47, which includes the first magnet 84 and the second magnet 85 arranged along the movement path of the stage 34. The holding sections 51L and 51R can be operated by a magnetic force in conjunction with the movement of the stage 34 without using a driven actuator or power source. It is possible to prevent wear and dust generation due to sliding between elements. It is possible to maintain clean conditions within the electrophoresis apparatus 1 and improve the maintenance-free performance of the electrophoresis apparatus 1. <Dritte Ausführungsform>

[0220] According to the third embodiment, the electrophoresis apparatus 1 uses the engaging element represented by engaging blades 87L and 87R that rotate around a central axis perpendicular to the horizontal direction. A rotating mechanism that rotates in conjunction with the movement of the stage 34 is used as a switching mechanism that switches the states of the engaging elements. Support posts 86L and 86R that move with the stage 34 support the engaging blades 87L and 87R together with the drive blades 88L and 88R as drive elements. A guide member 90 that guides the rotation of the drive blades 88L and 88R is provided on the movement path of the stage 34 in the X-axis direction.

[0221] Fig. 21 is a perspective view illustrating an autosampler provided for the electrophoresis apparatus. Fig. 21 shows a partially sectioned view of an example structure of the autosampler 25 included in the electrophoresis apparatus 1.

[0222] As in Fig. 6, the autosampler 25 includes, for example, the sliding section 50 that moves together with the table 34, the X-axis driving section 35, the Z-axis driving section 36, and the Y-axis driving section 37.

[0223] In the electrophoresis apparatus 1 according to the third embodiment, the support posts 86L and 86R are erected on the stage base 42. The support posts 86L and 86R are provided symmetrically on the left and right sides of the stage 34 to move integrally with the stage 34. The support posts 86L and 86R are provided over a length that protrudes upward above the stage 34. The support posts 86L and 86R are provided to be rotatable about their central axes along the longitudinal direction of the support posts 86L and 86R.

[0224] The support posts 86L and 86R support the engaging blades 87L and 87R and the driving blades 88L and 88R. The engaging blades 87L and 87R and the driving blades 88L and 88R are provided to extend laterally to protrude in a direction perpendicular to the center axes of the support posts 86L and 86R. Torsion springs 89L and 89R as torsionally elastic members are attached to the driving blades 88L and 88R. Rotation stoppers 91L and 91R are provided near the support posts 86L and 86R.

[0225] The engaging blades 87L and 87R are supported at a height that allows them to enter the holding nail engaging holes 33L and 33R of the sample adapter 29 placed on the stage 34. The engaging blades 87L and 87R rotate around and are rotatably supported by the support posts 86L and 86R to enter the holding nail engaging holes 33L and 33R. The engaging blades 87L and 87R enter and engage the holding nail engaging holes 33L and 33R to fix the sample plate assembly 28 on the stage 34.

[0226] The drive blades 88L and 88R are supported at a height that allows them to contact the guide member 90. When the stage 34 is transported backward on the X-axis drive base 43, the drive blades 88L and 88R contact the guide member 90 and rotate around the support posts 86L and 86R at a predetermined angle. The rotation of the drive blades 88L and 88R around the support posts 86L and 86R drives the engagement blades 87L and 87R to enter the holding nail engagement holes 33L and 33R.

[0227] The engaging blades 87L and 87R and the driving blades 88L and 88R can be formed into any suitable shape, such as a plate or rod. The engaging blades 87L and 87R are provided with a length that allows them to enter the holding pin engaging holes 33L and 33R provided in the side surface of the sample adapter 29.

[0228] The engaging blades 87L and 87R and the driving blades 88L and 88R are advantageously provided at an angle that allows them to be approximately perpendicular to each other when viewed from above. The angle between the engaging blade 87L or 87R and the driving blade 88L or 88R is advantageously set, for example, to 60 degrees or more and 120 degrees or less, advantageously 80 degrees or more and 100 degrees or less, and even more advantageously approximately 90 degrees. Such angles can enable the engaging blades 87L and 87R to mesh with the driving blades 88L and 88R and reliably enter the retaining nail engagement holes 33L and 33R.

[0229] It would be advantageous for the engaging blades 87L and 87R to be shaped to extend laterally and each include a flat bottom surface configured to be capable of contacting the lower inner wall of the retaining pin engaging holes 33L and 33R. Such a shape allows the engaging blades 87L and 87R, when they laterally enter the retaining pin engaging holes 33L and 33R, to exert a downward force on the lower inner wall of the retaining pin engaging holes 33L and 33R. The sample plate assembly 28 can be effectively prevented from lifting.

[0230] The torsion springs 89L and 89R are attached to the drive blades 88L and 88R at one end and to the stage base 42 at the other end. When the drive blades 88L and 88R rotate around the support posts 86L and 86R, the torsion springs 89L and 89R exert a force on the drive blades 88L and 88R around the support posts 86L and 86R, causing the drive blades 88L and 88R to return to their original positions. When the support posts 86L and 86R are rotatable, the torsion springs 89L and 89R drive the engagement blades 87L and 87R to be pulled out of the retaining nail engagement holes 33L and 33R.

[0231] The guide member 90 is provided above the back of the X-axis drive base 43. The guide member 90 is fixed above the X-axis drive base 43 to maintain a certain height from the top of the X-axis drive base 43. The guide member 90 is positioned along the X-axis direction to align its side surface parallel to the X-axis direction and maintain a height that does not interfere with the stage 34 transported on the X-axis drive base 43.

[0232] The guide member 90 guides the rotation of the drive blades 88L and 88R. The front end of the guide member 90 begins to guide the drive blades 88L and 88R. The side surfaces of the guide member 90 are parallel to the X-axis direction and hold the drive blades 88L and 88R at a predetermined angle around the support posts 86L and 86R. The front end of the guide member 90 is positioned behind the transfer area and in front of the connection area in the X-axis direction.

[0233] In Fig. 21, the guide member 90 is provided as a single flat plate. Alternatively, the guide member 90 may also be provided as two rails parallel to the X-axis direction on both the right and left sides of the stage base 42.

[0234] The rotation stops 91L and 91R are provided on the stage base 42. The rotation stops 91L and 91R are provided symmetrically on the right and left sides of the stage 34 near the support posts 86L and 86R. The rotation stops 91L and 91R are provided to protrude upward from the drive blades 88L and 88R. The drive blades 88L and 88R rotate due to a force applied by the torsion springs 89L and 89R and stop at a predetermined initial position by the rotation stops 91L and 91R.

[0235] Fig. 22A is a plan view illustrating the rotating mechanism in a released state. Fig. 22A is a plan view illustrating the rotating mechanism in an engaged state. Fig. 22A and Fig. 22B illustrate the operation of the rotating mechanism including the support posts 86L and 86R, the engaging blades 87L and 87R, and the driving blades 88L and 88R moving together with the stage 34 relative to the guide member 90. Fig. 22A and Fig. 22B correspond to a view from direction D in Fig. 21. In Fig. 22A and Fig. 22B, the sample mounting section 28, which is partially in perspective and mounted on the stage 34, is omitted.

[0236] As in Fig. 22A illustrates that when the stage 34 is positioned toward the transfer area, the drive blades 88L and 88R are aligned in the Y-axis direction and remain in their initial positions before rotating around the support posts 86L and 86R. The engagement blades 87L and 87R are aligned in the X-axis direction and remain in their initial positions before rotating around the support posts 86L and 86R. The drive blades 88L and 88R do not contact the guide member 90 and are not subjected to elastic force by the torsion springs 89L and 89R. This represents a released state in which the engagement blades 87L and 87R do not enter the holding pin engagement holes 33L and 33R of the sample adapter 29.

[0237] As in Fig. As shown in Fig. 22B, when the stage 34 is transported toward the connecting portion, the drive blades 88L and 88R move relative to the guide member 90 fixed to the X-axis drive base 43. The drive blades 88L and 88R contact the guide member 90 and rotate around the support posts 86L and 86R by a predetermined angle. The rotation of the drive blades 88L and 88R also causes the engagement blades 87L and 87R to rotate around the support posts 86L and 86R by a predetermined angle. This represents an engagement state in which the engagement blades 87L and 87R can enter the holding pin engagement holes 33L and 33R of the sample adapter 29.

[0238] When the stage 34 is transported toward the transfer area, the drive blades 88L and 88R do not contact the guide member 90, rotate around the support posts 86L and 86R due to the force of the torsion springs 89L and 89R, and contact the rotation stoppers 91L and 91R, returning to their initial positions. The rotation of the drive blades 88L and 88R also causes the engagement blades 87L and 87R to rotate around the support posts 86L and 86R, returning to their initial positions. This represents a disengaged state in which the engagement blades 87L and 87R do not enter the holding pin engagement holes 33L and 33R of the sample adapter 29.

[0239] Fig. Figure 23 is a diagram illustrating the relationship between the stage position in the X-axis direction and the engagement state of the engagement blades. Fig. In Figure 23, the horizontal axis indicates the position of the stage 34 in the X-axis direction, and the vertical axis indicates the engagement and disengagement states of the sample adapter 29 according to the rotation of the engagement blades 87L and 87R. The left end of the horizontal axis indicates the transfer area. The right end of the horizontal axis indicates the connection area. Fig. 23 the K position is aligned with the connection area.

[0240] As in Fig. As illustrated in Fig. 23, the engaging blades 87L and 87R vary the engagement state depending on how much they enter the holding nail engagement holes 33L and 33R, and switch between the engagement state and the disengaged state in conjunction with the movement of the stage 34 in the X-axis direction. The substantially constant disengaged state is maintained toward the transfer area in the X-axis direction. On the other hand, the approximately constant engagement state is maintained toward the connection area in the X-axis direction. The engagement state gradually varies while remaining in an intermediate state between the first and second positions located between the transfer area and the connection area.

[0241] The first position corresponds to the position of the front end of the guide member 90 in the X-axis direction, namely, the position where the engaging blades 87L and 87R complete the transition to the released state and begin the transition to the engaged state. Toward the transfer area from the first position, the engaging blades 87L and 87R are completely disengaged from the holding pin engagement holes 33L and 33R, thereby releasing the sample plate assembly 28 from the stage 34.

[0242] The second position corresponds to the position where the drive blades 88L and 88R, in contact with the guide member 90, rotate around the support posts 86L and 86R by the maximum angle, namely, the position where the engaging blades 87L and 87R complete the transition to the engaged state and begin the transition to the disengaged state. Toward the connection area from the second position, the engaging blades 87L and 87R fully enter the retaining nail engagement holes 33L and 33R, thereby securing the sample plate assembly 28 to the stage 34.

[0243] As in Fig. As illustrated in Figure 23, when multiple connection positions are specified, the second position is preferably located toward the transfer area away from all connection positions. This configuration can allow the engaging blades 87L and 87R to maintain the engaged state toward the side closer to the connection area away from the second position. The sample plate assembly 28 can be reliably mounted on the stage 34 at all connection positions. <Zusammenfassung der dritten Ausführungsform>

[0244] As above, the electrophoresis apparatus 1 according to the third embodiment includes the sample plate assembly 28, the stage 34, and the autosampler 25, similar to the electrophoresis apparatus 1 according to the first embodiment. The engaging blades 87L and 87R are included as engaging members capable of switching between the engaged state in which the sample plate assembly 28 is engaged with the stage 34 and the disengaged state in which the sample plate assembly 28 is disengaged from the stage 34. For example, the support posts 86L and 86R, the drive blades 88L and 88R, and the guide member 90 are included as switching mechanisms that allow the engaging blades 87L and 87R to switch between the engaged state and the disengaged state.

[0245] The electrophoresis apparatus 1 according to the third embodiment can achieve the same effect as the electrophoresis apparatus 1 according to the first embodiment based on the engaging blades 87L and 87R, the supporting posts 86L and 86R, the driving blades 88L and 88R, and the guide member 90.

[0246] For example, in the electrophoresis apparatus 1 according to the third embodiment, the switching mechanism uses the rotating mechanism composed of the support posts 86L and 86R, the driving blades 88L and 88R, and the guide member 90. The engaging blades 87L and 87R can operate in conjunction with the movement of the stage 34 without using a driven actuator or power source. The engaging blades 87L and 87R and the rotating mechanism operate in the horizontal direction, eliminating the need for a mechanism or space for operating in the vertical direction. It is possible to reduce the vertical thickness of the container fixing mechanism and ensure compactness around the stage 34.

[0247] Although specific preferred embodiments of the present invention have been described, the invention is not limited to the embodiments, but may be embodied in various ways within the spirit and scope of the invention. For example, the present invention is not limited to anything, which may include all configurations included in the embodiments described above. Part of the configuration of one embodiment may be replaced with another configuration, added to other embodiments, or omitted.

[0248] For example, in Fig. 19, the second magnet 85 is formed in an inclined shape. Without using this shape, the second magnet 85 can also be configured to increase the magnetic flux density to the transfer area on the upper surface. The second magnet 85 can consist of one or more magnets. Such a configuration can gradually raise the back of the vibrating element 56 based on changes in the magnetic force, regardless of the shape.

[0249] As in Fig. 21, the guide member 90 is provided toward the connection area or may be provided toward the transfer area in the X-axis direction with respect to the support posts 86L and 86R. When the guide member 90 is provided toward the transfer area with respect to the support posts 86L and 86R, it is possible to reverse and adjust the rotation directions of the support posts 86L and 86R and the positional relationship between the engaging blades 87L and 87R and the driving blades 88L and 88R. List of reference symbols 1 electrophoresis device 2 Capillary arrangement 3 Electrophoresis section 4 Liquid supply section 5 Irradiation detection section 6 capillaries 7 Capillary head 8 Charging head 9 Clamping plate 10 Cathode electrode 11 Cathode end 12 Detection position 13 cathode-side buffer tank 14 High-voltage supply 15 Buffer solution 16 Pump 17 Blocks 18 polymer containers 19 anode-side buffer tank 20 polymer solution 21 Anode electrode 22 Light source 23 Detector 24 housings 25 autosamplers 26 Buffer transport section 27 Sample assembly section 28 Sample plate assembly (container) 29 sample adapters 30 sample plates (containers) 31 Septum 32 Septum clip 33 Retaining nail hole 34 Stage 35 X-axis drive section 36 Z-axis drive section 37 Y-axis drive section 38 Y-axis guide rail 39 X-axis guide rail 40 slot section 41 Sample mounting section rail 42 Stage base 43 X-axis drive base 44 X-axis guide rail 45 Power source 46 X-axis drive belts 47 Mechanism for opening and closing the holding section (switching mechanism) 48 Drive connecting element 49 Positioning pin 50 sliding section 51 Holding section (engaging element) 52 Holding section base element 53 first support shaft hole 54 socket 55 first support shaft 56 Oscillating element 57 second support shaft hole 58 Cam roller pin hole 59 Cam roller pin 60 cam roller 61 Press section 62 stop 63 compression spring 64 third support shaft hole 65 second support shaft 66 fourth support shaft hole 67 Retaining nail (displacement suppression section) 68 spring hook section 69 tension spring 70 pressure projection section 71 X-axis slider 72 cam element 73 inclined cam surface 74 cam surface 75 K position 76 L-position 77 M-Position 78 N-Position 79 inclined surface 80 curved section 81 Power source 82 Z-axis drive belts 83 thermostatic bath 84 first magnet (first magnetic element) 85 second magnet (second magnetic element) 86 support posts 87 engagement blade (engagement element) 88 drive blade 89 torsion spring 90 guide element 91 Rotation stop 92 nails 93 Magnetic pole (N-pole) 94 Magnetic pole (S-pole) QUOTES CONTAINED IN THE DESCRIPTION

[0000] This list of documents submitted by the applicant was generated automatically and is included solely for the convenience of the reader. This list is not part of the German patent or utility model application. The DPMA assumes no liability for any errors or omissions. Cited patent literature

[0000] US 6902703

[0012] WO 2021 / 26951

[0012] JP 2022-035426

[0012] < / elektrophoreseanalyseverfahren>

Claims

[1] Electrophoresis device with a capillary, a container for holding a sample or reagent, a platform for attaching the container, and a drive section for driving the stage at least in a horizontal direction, the electrophoresis device further comprising: an engagement member that can switch between an engaged state for engaging the container with the stage and a released state for releasing the container from the stage; and a switching mechanism that enables the engagement element to switch between the engaged state and the released state, wherein, along a path of movement of the stage, one side has a transfer area capable of transferring the container to the stage, the other side has a connection area capable of connecting and removing the capillary from the container, a first position is provided between one side and the other side, and a second position is provided between the first position and the other side; wherein the switching mechanism allows the engagement member to enter the released state when a state of the engagement member transitions in conjunction with the movement of the stage between the first position and the second position to position the stage to one side; wherein the engaging member enters the engaged state when the stage is positioned to the other side; and wherein the stage moves horizontally between one side and the first position while the engagement member is held in the engaged state, and moves horizontally between the other side and the second position while the engagement member is held in the released state. [2] The electrophoresis apparatus according to claim 1, wherein the switching mechanism gradually changes the state of the engaging member as the stage moves between the first position and the second position. [3] Electrophoresis device according to claim 1, comprising: a drive section for driving the stage in a vertical direction, wherein, when the stage is positioned to one side, the engaging member rises while maintaining the engaged state to allow the capillary to be connected to the container, and after electrophoresis using the capillary, the engaging member lowers while maintaining the engaged state to allow the capillary to be removed from the container; and wherein the stage is driven to lower such that the engaging member generates a downward force greater than a frictional force generated between the capillary and the container. [4] The electrophoresis apparatus according to claim 3, wherein the engaging member has a horizontally extendable displacement suppressing portion that prevents the container mounted on the stage from being lifted upward due to the frictional force. [5] Electrophoresis device according to claim 4, wherein the container has a pair of right and left side surfaces parallel to the horizontal movement direction of the stage and is formed in a rectangular parallelepiped shape with an open bottom surface; wherein the right and left side surfaces have a pair of through holes into which the displacement suppressing portion can be inserted; wherein the engagement element extends from directly below the container, is positioned within the outer edge of the container according to a plan view of the container, passes through the open bottom surface and extends into the through-hole; and wherein the displacement suppressing portion can switch between an engaged state to be inserted into the through hole from the inside to the outside and a released state to be pulled out toward the inside of the through hole. [6] Electrophoresis device according to claim 5, wherein the engagement member has the displacement suppressing portion and a bent portion provided at the tip end of the displacement suppressing portion and protruding in a direction perpendicular to the displacement suppressing portion; wherein the bent portion has an inclined surface whose projection width projects in a direction perpendicular to the displacement suppressing portion and increases from the base end to the tip end of the displacement suppressing portion; and wherein, when the displacement suppressing portion remains in the engaged state, the inclined surface is positioned outside the through-hole according to a plan view of the container, and when the displacement suppressing portion is to be pulled out from the through-hole, it contacts the periphery of the through-hole, thereby generating a reaction force in a direction in which the displacement suppressing portion is inserted into the through-hole. [7] An electrophoresis device according to claim 4, wherein the switching mechanism comprises: a swing member having a support shaft extending in a direction perpendicular to the horizontal movement direction of the stage, which is pivotally provided so that one side and the other side alternately rock around the support shaft, and having a cam follower toward the one side, and an inclined cam surface provided obliquely between the first position and the second position; wherein the engagement member, which has a support shaft extending in a direction parallel to the horizontal movement direction of the stage, is pivotally provided so that one side and the other side alternately rock around the support shaft, and has the displacement suppression portion toward the one side, and wherein the cam follower rises due to the movement of the stage along the inclined cam surface, the rising cam follower lowers the other side of the swing member to press the other side of the engagement member, thereby causing one side of the engagement member to rise, and the displacement suppressing portion thereby enters the engagement state. [8] An electrophoresis device according to claim 4, wherein the switching mechanism comprises: a swing member having a support shaft extending in a direction perpendicular to the horizontal movement direction of the stage, which is pivotally provided so that one side and the other side alternately rock around the support shaft, and having a first magnetic element toward the one side, and a second magnetic element provided between the first position and the second position; wherein the engagement member, which has a support shaft extending in a direction parallel to the horizontal movement direction of the stage, is pivotally provided so that one side and the other side alternately rock around the support shaft, and has the displacement suppression portion toward the one side, and wherein the movement of the stage causes the first magnetic member and the second magnetic member to repel each other by magnetic force, the magnetic repulsion causes the other side of the swing member to be lowered and presses the other side of the engagement member, thereby causing one side of the engagement member to rise, and the displacement suppressing portion thereby enters the engagement state. [9] An electrophoresis device according to claim 4, wherein the switching mechanism comprises: a support post erected on the side of the stage and designed to rotate around its central axis, a drive element extending laterally from the support post, a guide element provided between the first position and the second position to maintain a height that allows contact with the drive element, and a torsionally elastic element which exerts a force on the drive element in a direction opposite to the rotation of the drive element about the support post, wherein the engagement member is supported to extend laterally from the support post, wherein the stage moves to move the drive member along the guide member and rotate the support post, thereby causing the engagement member to rotate around the support post, and thereby causing the displacement suppressing portion to enter the engaged state.

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