METHOD FOR MEASURING TOTAL PROTEIN CONTENT AND DETECTING PROTEIN BY MEANS OF IMMUNEST IN A MICROGLUIDIC DEVICE
Patent Information
- Application Number
- DE602021055238
- Authority / Receiving Office
- DE · DE
- Patent Type
- Patents
- Current Assignee / Owner
- Priority Date
- 2020-07-17
- Filing Date
- 2021-04-14
- Publication Date
- 2026-06-03
- Estimated Expiration
- 2041-04-14
AI Technical Summary
Existing systems for capillary-based immunoassays lack the capability to perform chemiluminescence detection for both immunoassay and Total Protein readout in the same capillary, and they are limited in multiplexing capabilities, leading to constraints in antibody mixing due to cross-reactivity and incompatible dynamic ranges.
Incorporating a stripping reagent with Tris(2-carboxyethyl)phosphine hydrochloride (TCEP) at a pH between 3.0 and 4.5 to remove primary antibodies, allowing for sequential immunoassays and Total Protein assays in the same capillary, utilizing chemiluminescence and fluorescence detection for multiplexed analysis.
Enables high sensitivity total protein measurements and immunoassays with low sample requirements, improving throughput and accuracy by normalizing immunoassay signals to total protein content, reducing the need for additional samples and saving time and resources.