Detergents and cleaning agents with an improved enzyme stability

Abstract

The invention relates to detergents and cleaning agents, comprising (A) at least one protease, which has an amino acid sequence that is at least 70% identical to the amino acid sequence specified in SEQ ID NO:1 over the entire length thereof and has, with respect to the numbering according to SEQ ID NO:1 in each case, (i) at least two amino acid substitutions at at least two of the positions corresponding to the positions 3, 4, 99, or 199 and (ii) at least one additional amino acid substitution at least one of the positions corresponding to the positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209, 212, or 256, (B) at least one compound with the formula (I) or (II), wherein in each case R is selected from -COOH, C1-6-alkyl-substituted or unsubstituted C2-6-dicarboxylic acids, C1-6-alkyl-substituted or unsubstituted C2-6-dicarboxylic acids, and -OOC-NR2 2, and R2 are the same or different and are selected from C1-6-alkyl or H, as well as salts, esters, or derivatives thereof, (C) optionally at least one additional enzyme, and (D) at least one ingredient. The invention likewise relates to the corresponding washing and cleaning methods, to the use of the agents described herein, and to the use of an inhibitor according to the invention for improving the stability of a protease and / or optionally additional enzymes in detergents or cleaning agents.

Description

[0001] DETERGENT AND CLEANING AGENTS WITH IMPROVED ENZYME STABILITY

[0002] The invention lies in the field of enzyme technology. The invention relates to washing or cleaning agents comprising at least one protease, at least one reversible protease inhibitor, and optionally at least one further enzyme. The invention also encompasses the corresponding washing and cleaning processes, the use of the agents described herein, and the use of reversible protease inhibitors according to the invention to improve the stability of a protease in washing or cleaning agents.

[0003] The use of enzymes in detergents has been established in the state of the art for decades. They serve to expand the performance spectrum of the respective agents according to their special activities. These include, in particular, hydrolytic enzymes such as proteases, amylases, lipases, and cellulases. The first three hydrolyze proteins, starch, and fats and thus contribute directly to soil removal. Cellulases are used in particular for their effect on fabrics. Another group of detergent enzymes are oxidative enzymes, particularly oxidases, which, if necessary in conjunction with other components, preferably serve to bleach soils or generate the bleaching agents in situ. In addition to these enzymes, which are subject to continuous optimization, further enzymes are constantly being made available for use in detergents in order to optimally address special types of soiling, such as:Pectinases, ß-glucanases, mannanases or other hemicellulases (glycosidases) for the hydrolysis of special plant polymers in particular.

[0004] Proteases are among the most technically important enzymes. They are the longest-established enzymes in detergents and cleaning agents and are contained in virtually all modern, high-performance detergents and cleaning agents. In detergents and cleaning agents, proteases serve to break down protein-containing soiling on the items being cleaned. However, they also hydrolyze themselves (autoproteolysis) and all other proteins contained in the agents in question, i.e. in particular other enzymes contained in the detergents and cleaning agents. This occurs particularly during the cleaning process, i.e. in the aqueous washing or cleaning liquor, when comparatively favorable reaction conditions exist. This also occurs to a lesser extent during storage of the agents in question, which is why long storage is always accompanied by a certain loss of protease activity and the activity of the other enzymes.Due to the loss of enzymatic activity, they no longer perform optimally. This is particularly problematic in gel or liquid formulations, and especially in water-based formulations, because the water contained in these formulations provides both the reaction medium and the hydrolysis reagent.

[0005] In general, only selected proteases are suitable for use in liquid surfactant-containing preparations. Many proteases either do not exhibit sufficient catalytic performance in such preparations or are not sufficiently stable. Therefore, high catalytic activity and stability under conditions such as those encountered during a washing process are particularly desirable for the use of proteases in detergents.Examples of the subtilisin-type proteases preferably used in detergents and cleaning agents are the subtilisins BPN' from Bacillus amyloliquefaciens and Carlsberg from Bacillus licheniformis, the protease PB92, the subtilisins 147 and 309, the protease from Bacillus lentus, in particular from Bacillus lentus DSM 5483, subtilisin DY, and the enzymes thermitase, proteinase K, and the proteases TW3 and TW7, which are classified as subtilases but no longer as subtilisins in the narrower sense, as well as variants of the aforementioned proteases that have an amino acid sequence that is altered compared to the parent protease. Proteases are modified in a targeted or random manner using methods known from the state of the art and are thus optimized, for example, for use in detergents and cleaning agents. These include point, deletion or insertion mutagenesis or fusion with other proteins or protein parts.For most proteases known from the prior art, optimized variants are known. For example, EP 2016175 discloses a protease from Bacillus pumilus intended for detergents and cleaning agents. WO 2013 / 060621 and EP 3660151 describe protease variants of the alkaline protease from Bacillus lentus optimized for detergents and cleaning agents.

[0006] One goal in the development of detergent and cleaning agent formulations is therefore to stabilize the enzymes they contain, particularly during storage, and to protect them from denaturation and / or cleavage or degradation and / or decomposition due to physical influences or oxidation, etc., particularly during storage and / or use of the detergent or cleaning agent. One focus of these developments is to protect the proteins and / or enzymes they contain against (auto-)proteolytic cleavage. This can be achieved by creating physical barriers, for example by encapsulating the enzymes in special enzyme granules or by packaging the agents in two- or multi-chamber systems. The other frequently used approach is to add chemical compounds that inhibit the proteases and thus act as stabilizers or inhibitors for proteases and the other proteins and enzymes they contain.However, these must be reversible protease inhibitors, since the protease activity should only be inhibited temporarily, especially during storage, but not during the purification process.

[0007] Various reversible protease inhibitors are described in the prior art, e.g., polyols, particularly glycerol and 1,2-propylene glycol, benzamidine hydrochloride, borax, boric acids, boronic acids, or their salts or esters. The use of boric acid derivatives together with polyols is also known. 4-Formylphenylboronic acid (4-FPBA) is also a protease inhibitor known from the prior art. Peptide aldehydes, i.e., oligopeptides with a reduced C-terminus, particularly those consisting of 2 to 50 monomers, have also been described for this purpose. Peptide reversible protease inhibitors include, among others, ovomucoid and leupeptin. Specific, reversible peptide inhibitors and fusion proteins composed of proteases and specific peptide inhibitors are also used for this purpose.

[0008] However, there is still a need to improve the cleaning performance of enzyme-containing detergents and cleaning agents, as well as to better stabilize the enzymes contained in these detergents and cleaning agents. In particular, there is a growing demand to eliminate boric acid-containing compounds, especially boron-containing protease inhibitors, from detergents and cleaning agents.

[0009] Surprisingly, it has now been found that certain compounds with the formula (I) or where R is each selected from -COOH, Ci-e-alkyl-substituted or unsubstituted C2-6-dicarboxylic acids, Ci-e-alkyl-substituted or unsubstituted C2-e-carboxylic acids and -OOC-NR 2 2, where R 2are identical or different and selected from Ci-e-alkyl or H, as well as salts, esters or derivatives thereof, can advantageously stabilize and reversibly inhibit a protease and are therefore particularly suitable for use in washing or cleaning agents. In particular, it was surprisingly found that a protease which comprises an amino acid sequence which is at least 70% identical to the amino acid sequence given in SEQ ID NO:1 over its entire length and, in each case based on the numbering according to SEQ ID NO:1 (i) at least three, preferably four of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions, preferably four amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least one of the positions corresponding to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209, 212 or 256,at least one amino acid substitution selected from the group consisting of N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G, Y161T, A163G, V171L, A181D, F183R, Q185R, Q200A / L / S / T, Y203KA / / W, A209K / W, N212S / T and L256D / E / Q, preferably S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E, by certain compounds of formula (I) or (II), where R is each selected from -COOH, Ci-e-alkyl-substituted or unsubstituted C2-6-dicarboxylic acids, Ci-e-alkyl-substituted or unsubstituted C2-e-carboxylic acids and -OOC-NR 2 2, where R 2 are identical or different and selected from Ci-e-alkyl or H, as well as salts, esters or derivatives thereof, can be advantageously stabilized and this combination is therefore particularly suitable for use in washing or cleaning agents.

[0010] The invention therefore relates, in a first aspect, to a washing and cleaning agent, in particular a liquid washing and cleaning agent, particularly preferably a liquid textile washing agent, comprising

[0011] (A) at least one protease, wherein the protease has proteolytic activity and comprises an amino acid sequence which is at least 70% identical to the amino acid sequence given in SEQ ID NO:1 over its entire length and, in each case based on the numbering according to SEQ ID NO:1 (i) at least three, preferably four of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions, preferably four amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least one of the positions corresponding to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209, 212 or 256, at least one Amino acid substitution selected from the group consisting of N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G, Y161T, A163G, V171L, A181D, F183R, Q185R, Q200A / L / S / T, Y203KA / / W, A209K / W, N212S / T and L256D / E / Q, preferably S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E, preferably in an amount of 0.0001 to 1% by weight, based on the total weight of the washing and cleaning agent,

[0012] (B) at least one compound of formula (I) or (II) where R is each selected from -COOH, Ci-e-alkyl-substituted or unsubstituted C2-6-dicarboxylic acids, Ci-e-alkyl-substituted or unsubstituted C2-e-carboxylic acids and -OOC-NR 2 2, where R 2 are the same or different and selected from Ci-e-alkyl or H, and salts, esters or derivatives thereof, preferably in an amount of 0.1 to 2 wt.%, based on the total weight of the washing and cleaning agent,

[0013] (C) optionally at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase, preferably in an amount of 0.0001 to 1 wt.%, based on the total weight of the washing and cleaning agent, and

[0014] (D) at least one ingredient, preferably in an amount of 0.01 to 99.9% by weight, based on the total weight of the washing and cleaning agent.

[0015] Another subject of the invention is a washing and cleaning agent, in particular a liquid washing and cleaning agent, particularly preferably a liquid textile washing agent, comprising

[0016] (A) at least one protease, wherein the protease has proteolytic activity and comprises an amino acid sequence which is at least 70% identical to the amino acid sequence given in SEQ ID NO:1 over its entire length and, in each case based on the numbering according to SEQ ID NO:1 (i) at least three, preferably four of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions, preferably four amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least one of the positions corresponding to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209, 212 or 256, at least one Amino acid substitution selected from the group consisting of N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G, Y161T, A163G, V171L, A181D, F183R, Q185R, Q200A / L / S / T, Y203KA / / W, A209K / W, N212S / T and L256D / E / Q, preferably S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E, preferably in an amount of 0.0001 to 1 wt.%, based on the total weight of the washing and cleaning agent,

[0017] (B) at least one compound selected from the group consisting of benzoic acid, phenylmalonic acid, benzylmalonic acid, phenylsuccinic acid, benzylsuccinic acid, methyl 3-benzoylpropionate, (S)-3-phenylbutyric acid and benzylcarbamate, preferably in an amount of 0.1 to 2% by weight, based on the total weight of the washing and cleaning agent,

[0018] (C) optionally at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase, preferably in an amount of 0.0001 to 1 wt.%, based on the total weight of the washing and cleaning agent, and

[0019] (D) at least one ingredient, preferably in an amount of 0.01 to 99.9% by weight, based on the total weight of the washing and cleaning agent.

[0020] Further aspects of the invention relate to processes for producing such a washing or cleaning agent, processes for cleaning textiles and / or hard surfaces, in particular dishes, using an agent according to the invention.

[0021] Another object of the invention relates to the use of a compound having the formula

[0022] (I) or (II) where R is each selected from -COOH, Ci-e-alkyl-substituted or unsubstituted C2-6-dicarboxylic acids, Ci-e-alkyl-substituted or unsubstituted C2-e-carboxylic acids and -OOC-NR 2 2, where R 2are the same or different and selected from C 1-8 alkyl or H, and salts, esters or derivatives thereof, for improving the stability, in particular the storage stability, of enzymes, preferably proteases, cellulases and / or amylases, in a protease-containing washing and cleaning agent, wherein the protease is the protease defined herein, preferably in a temperature range from about 20°C to about 60°C, particularly preferably from about 20°C to about 40°C, most particularly preferably at about 30°C, and the use of a compound having the formula (I) or (II) where R is each selected from -COOH, Ci-e-alkyl-substituted or unsubstituted C2-6-dicarboxylic acids, Ci-e-alkyl-substituted or unsubstituted C2-e-carboxylic acids and -OOC-NR 2 2, where R 2are the same or different and selected from Ci-e-alkyl or H, and salts, esters or derivatives thereof, for preventing and / or reducing the autoproteolysis of a protease contained in the washing and cleaning agent, wherein the protease is the protease defined herein, and the use of a compound having the formula (I) or (II) where R is each selected from -COOH, Ci-e-alkyl-substituted or unsubstituted C2-6-dicarboxylic acids, Ci-e-alkyl-substituted or unsubstituted C2-e-carboxylic acids and -OOC-NR 2 2, where R 2are identical or different and selected from C 1-8 alkyl or H, as well as salts, esters or derivatives thereof, for preventing and / or reducing the proteolysis of other enzymes present in the washing and cleaning agent, preferably amylases and / or cellulases, by a protease present in the washing and cleaning agent, wherein the protease is the protease defined herein. Preferably, the compound is selected from the group consisting of benzoic acid, phenylmalonic acid, benzylmalonic acid, phenylsuccinic acid, benzylsuccinic acid, methyl 3-benzoylpropionate, (S)-3-phenylbutyric acid and benzylcarbamate.

[0023] The detergent according to the invention is preferably a liquid textile detergent. Further preferably, the detergent according to the invention, in a 1 wt. % solution in deionized water at 20°C, has a pH in a range from about 6 to about 11, in particular from about 6.5 to about 10.5, further preferably from about 7 to about 10, particularly preferably from about 8 to about 9.

[0024] Textile detergents according to the invention exhibit performance advantages over other textile detergents, particularly when the textile detergents contain at least one additional enzyme of the same or a different type, e.g., amylase, cellulase, lipase, mannanase, or pectinase, although this list of additional enzymes is not exhaustive. Therefore, it is preferred that the textile detergents according to the invention contain at least one additional enzyme of the same type (i.e., another protease) or a different type, preferably selected from amylase, cellulase, and lipase, preferably cellulase.

[0025] These and other aspects, features, and advantages of the invention will become apparent to those skilled in the art from a study of the following detailed description and claims. Any feature of one aspect of the invention may be employed in any other aspect of the invention. Furthermore, it is to be understood that the examples contained herein are intended to describe and illustrate the invention, but not to limit it, and in particular, the invention is not limited to these examples.

[0026] Unless otherwise stated, all percentages are by weight (wt%).

[0027] Numerical ranges specified in the format "from x to y" include the specified values. If multiple preferred numerical ranges are specified in this format, it goes without saying that all ranges resulting from the combination of the different endpoints will also be included.

[0028] "At least one" as used herein means one or more, i.e. 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14 or more.

[0029] The term "washing and cleaning agent" or "washing or cleaning agent" as used herein is synonymous with the term "agent" and refers to a composition for cleaning textiles and / or hard surfaces, in particular dishes, as explained in the description.

[0030] "Approximately," "approximately," or "approximately," as used herein with reference to a numerical value, refers to the corresponding numerical value ±10%, preferably ±5%.

[0031] "Substantially free from" means that the composition or agent contains less than 2% by weight, preferably less than 1% by weight, more preferably less than 0.5% by weight and most preferably less than 0.1% by weight of the corresponding substance, based on the total weight of the composition / agent.

[0032] "Liquid," as used herein, includes liquids and gels, as well as pasty compositions. It is preferred that the liquid compositions be flowable and pourable at room temperature, but it is also possible for them to have a yield point. A substance, e.g., a composition or an agent, is solid according to the definition of the invention if it is in the solid state at 25°C and 1013 mbar. A substance, e.g., a composition or an agent, is liquid according to the definition of the invention if it is in the liquid state at 25°C and 1013 mbar. Liquid also includes gel.

[0033] "Variant", as used herein, refers to natural or artificially produced variations of a native protease that have a modified amino acid sequence compared to the reference form.

[0034] An "improvement in the stability of an enzyme" within the meaning of the invention exists if the presence of a compound (B) defined herein with the formula (I) or (II) causes a washing or cleaning agent comprising at least one protease (A) defined herein and at least one compound (B) defined herein with the formula (I) or (II) (washing or cleaning agent according to the invention) to have a higher enzymatic activity of the protease and / or optionally of other enzymes contained in the washing or cleaning agent after storage compared to a control preparation which differs from the washing or cleaning agent according to the invention only by the absence of the compound defined herein with the formula (I) or (II) (control). After storage, the washing or cleaning agent according to the invention therefore has a higher residual activity of the protease and / or optionally of other enzymes contained.of other enzymes contained compared to the control, wherein the washing or cleaning agent according to the invention and the control have the same initial enzymatic activity at the start of storage, both agents are treated in the same way, in particular with regard to the storage conditions and the determination of enzyme activity. Storage is increasingly preferably for at least 1 week, 2 weeks, 3 weeks, or 4 weeks. Storage is further preferably at a temperature of 20°C, 25°C, 30°C, or 40°C.

[0035] The present invention is based on the surprising finding of the inventors that the compounds described herein with the formula (I) or (II) where R is each selected from -COOH, Ci-e-alkyl-substituted or unsubstituted C2-6-dicarboxylic acids, Ci-e-alkyl-substituted or unsubstituted C2-e-carboxylic acids and -OOC-NR 2 2, where R 2are identical or different and selected from C 1-8 alkyl or H, as well as salts, esters or derivatives thereof, improve the storage stability of proteases defined herein in detergents and cleaning agents. This is particularly surprising since these compounds defined herein have the formula (I) or (II) (II), wherein R is each selected from -COOH, Ci-e-alkyl-substituted or unsubstituted C2-6-dicarboxylic acids, Ci-e-alkyl-substituted or unsubstituted C2-e-carboxylic acids and -OOC-NR 2 2, where R 2identical or different and selected from C 1-8 alkyl or H, as well as salts, esters or derivatives thereof, have not previously been associated with reversible protease inhibition. In particular, they have not previously been associated with the proteases described herein and with improved storage stability and / or reduced autoproteolysis. Furthermore, the compounds described herein with the formula (I) or (II) where R is each selected from -COOH, Ci-e-alkyl-substituted or unsubstituted C2-6-dicarboxylic acids, Ci-e-alkyl-substituted or unsubstituted C2-e-carboxylic acids and -OOC-NR 2 2, where R 2 are the same or different and selected from Ci-e-alkyl or H, as well as salts, esters or derivatives thereof, have not been associated with reversible inhibition of the protease described herein.

[0036] The present invention is based in particular on the surprising finding of the inventors that a protease which comprises an amino acid sequence which is at least 70% identical to the amino acid sequence given in SEQ ID NO:1 over its entire length and, in each case based on the numbering according to SEQ ID NO:1 (i) at least three, preferably four of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions, preferably four amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least one of the positions corresponding to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209, 212 or 256, Amino acid substitution selected from the group consisting of N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G, Y161T, A163G, V171L, A181D, F183R, Q185R, Q200A / L / S / T, Y203K / V / W, A209K / W, N212S / T and L256D / E / Q, preferably S154D, S160G, A181D, F183R,Q185R, Q200L, Y203W, A209K and L256E, by compounds of formula (I) or (II), where R is each selected from -COOH, Ci-e-alkyl-substituted or unsubstituted C2-6-dicarboxylic acids, Ci-e-alkyl-substituted or unsubstituted C2-e-carboxylic acids and -OOC-NR 2 2, where R 2 identical or different and selected from C 1-8 alkyl or H, as well as salts, esters or derivatives thereof, can advantageously be stabilized or reversibly inhibited in washing and cleaning agents. This is particularly surprising insofar as none of these compounds defined herein have the formula (I) or (II) (II), wherein R is each selected from -COOH, Ci-e-alkyl-substituted or unsubstituted C2-6-dicarboxylic acids, Ci-e-alkyl-substituted or unsubstituted C2-e-carboxylic acids and -OOC-NR 2 2, where R 2identical or different and selected from C 1-8 alkyl or H, as well as salts, esters or derivatives thereof, have previously been associated with improved stability or reversible inhibition of such a protease in detergents or cleaning agents. Furthermore, such compounds defined herein with the formula (I) or (II) where R is each selected from -COOH, Ci-e-alkyl-substituted or unsubstituted C2-6-dicarboxylic acids, Ci-e-alkyl-substituted or unsubstituted C2-e-carboxylic acids and -OOC-NR 2 2, where R 2 are the same or different and selected from Ci-e-alkyl or H, and salts, esters or derivatives thereof, have not previously been associated with improved stability of other enzymes contained in the washing or cleaning agent when they are present in the washing or cleaning agent together with the protease described herein.

[0037] The present invention is further based in particular on the surprising finding of the inventors that a protease which comprises an amino acid sequence which is at least 70% identical to the amino acid sequence given in SEQ ID NO:1 over its entire length and, in each case based on the numbering according to SEQ ID NO:1 (i) at least three, preferably four of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions, preferably four amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least one of the positions corresponding to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209, 212 or 256, Amino acid substitution selected from the group consisting of N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G, Y161T, A163G, V171L, A181D, F183R, Q185R, Q200A / L / S / T, Y203KA / / W, A209K / W, N212S / T and L256D / E / Q, preferably S154D, S160G, A181D,F183R, Q185R, Q200L, Y203W, A209K, and L256E, can be advantageously stabilized in washing and cleaning agents by a compound selected from the group consisting of benzoic acid, phenylmalonic acid, benzylmalonic acid, phenylsuccinic acid, benzylsuccinic acid, methyl 3-benzoylpropionate, (S)-3-phenylbutyric acid, and benzylcarbamate. This is particularly surprising insofar as none of these compounds has previously been associated with improved stability of such a protease in washing or cleaning agents. Furthermore, such compounds have not previously been associated with improved stability of other enzymes contained in the washing or cleaning agent when they are present in the washing or cleaning agent together with the protease described herein.

[0038] According to the invention, compounds of formula (I) or (II) where R is each selected from -COOH, Ci-e-alkyl-substituted or unsubstituted C2-6-dicarboxylic acids, Ci-e-alkyl-substituted or unsubstituted C2-e-carboxylic acids and -OOC-NR 2 2, where R 2 are identical or different and selected from C 1-8 alkyl or H, as well as salts, esters, or derivatives thereof, are used as reversible protease inhibitors. In preferred embodiments, such compounds are boron-free, i.e., they do not contain any boron-containing substituents. Preferably, the compound is selected from the group consisting of benzoic acid, phenylmalonic acid, benzylmalonic acid, phenylsuccinic acid, benzylsuccinic acid, methyl 3-benzoylpropionate, (S)-3-phenylbutyric acid, and benzylcarbamate.

[0039] The agents according to the invention may comprise one or more reversible protease inhibitor(s).

[0040] The agents according to the invention may contain the reversible protease inhibitor(s) in a concentration of 0.1 to 2 wt.%, preferably 0.3 to 1.5 wt.%. If multiple inhibitors are present, these figures refer to the total concentration.

[0041] In preferred embodiments, the protease contained in the agents according to the invention is a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length to at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three, preferably four of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions, preferably four amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least one and increasingly preferably two, three, four, five or six of the positions,which correspond to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209, 212 or 256, at least one and increasingly preferably two, three, four, five or six amino acid substitution selected from the group consisting of N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G, Y161T, A163G, V171L, A181D, F183R, Q185R, Q200A / L / S / T, Y203KA / / W, A209K / W, N212S / T and L256D / E / Q, preferably S154D, S160G, A181 D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0042] In further preferred embodiments, the protease contained in the agents according to the invention is a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length to at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least one of the positions corresponding to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209, 212 or 256,at least one amino acid substitution selected from the group consisting of N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G, Y161T, A163G, V171L, A181D, F183R, Q185R, Q200A / L / S / T, Y203KA / / W, A209K / W, N212S / T and L256D / E / Q, preferably S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E.

[0043] In further preferred embodiments, the protease contained in the agents according to the invention is a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length to at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least one of the positions corresponding to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209, 212 or 256, at least one amino acid substitution selected from the group consisting of N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G,Y161T, A163G, V171L, A181D, F183R, Q185R, Q200A / L / S / T, Y203KA / / W, A209K / W, N212S / T and L256D / E / Q, preferably S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E.

[0044] In further preferred embodiments, the protease contained in the agents according to the invention is a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length to at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least one of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least one amino acid substitution,selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E.

[0045] In further preferred embodiments, the protease contained in the agents according to the invention is a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length to at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least two of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least two amino acid substitutions,selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E.

[0046] In further preferred embodiments, the protease contained in the agents according to the invention is a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length to at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least three of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least three amino acid substitutions,selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E.

[0047] In further preferred embodiments, the protease contained in the agents according to the invention is a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length to at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least four of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least four amino acid substitutions,selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E.

[0048] In further preferred embodiments, the protease contained in the agents according to the invention is a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length to at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least five amino acid substitutions at least five of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256,selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E.

[0049] In further preferred embodiments, the protease contained in the agents according to the invention is a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length to at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least six of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least six amino acid substitutions,selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E.

[0050] In further preferred embodiments, the protease contained in the agents according to the invention is a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length to at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least one of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least one amino acid substitution selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E existing group selected.,

[0051] In further preferred embodiments, the protease contained in the agents according to the invention is a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length to at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least two of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least two amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E existing group selected.,

[0052] In further preferred embodiments, the protease contained in the agents according to the invention is a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length to at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least three of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least three amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E existing group selected.,

[0053] In further preferred embodiments, the protease contained in the agents according to the invention is a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length to at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least four of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least four amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E existing group selected.,

[0054] In further preferred embodiments, the protease contained in the agents according to the invention is a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length to at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least five of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least five amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected. In further preferred embodiments, the protease contained in the agents according to the invention is a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length to at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least six of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least six amino acid substitutions which are selected from the group consisting of S154D, S160G,A181 D, F183R, Q185R, Q200L, Y203W, A209K and L256E.

[0055] In particularly preferred embodiments, the detergent according to the invention contains a protease with one of the following amino acid substitution variants:

[0056] (i) S3T-V4I-R99E-V199I-Q200L-Y203W;

[0057] (ii) S3T-V4I-R99E-V199I-N212S;

[0058] (iii) S3T-V4I-R99E-V199I-N74D;

[0059] (iv) S3T-V4I-R99E-V199I-S154D-L256E;

[0060] (v) S3T-V4I-R99E-V199I-Q200L-Y203W-S154D-L256E;

[0061] (vi) S3T-V4I-R99E-V199I-N74D-Q200L-Y203W;

[0062] (vii) S3T-V4I-R99E-V199I-N74D-S154D-Q200L-Y203W-L256E;

[0063] (viii) S3T-V4I-R99E-V199I-N74D-N212S;

[0064] (ix) S3T-V4I-R99E-V199I-N74D-S154D-Y203W-L256E;

[0065] (x) S3T-V4I-R99E-V199I-N74D-Y203W;

[0066] (xi) S3T-V4I-R99E-V199I-N74D-S154D-Q200L-L256E;

[0067] (xii) S3T-V4I-R99E-V199I-N74D-Q200L;

[0068] (xiii) S3T-V4I-R99E-V199I-S154D-Q200L-Y203W;

[0069] (xiv) S3T-V4I-R99E-V199I-Q200L-Y203W-L256E;

[0070] (xv) S3T-V4I-R99E-V199I-A136Q-R143W-Y161 T-Q200L;

[0071] (xvi) S3T-V4I-R99E-V199I-N74D-R143Y-A209W-N212S-L256E;

[0072] (xvii)S3T-V4l-R99E-V199I-A136Q-S154D-V171 L-Q200L;

[0073] (xviii) S3T-V4I-R99E-V199I-Q200L-Y203W-A209K-S154D-L256E;

[0074] (xix) S3T-V4I-R99E-V199I-S154D-S160G-Q185R-Q200L-Y203W-L256E;

[0075] (xx) S3T-V4I-R99E-V199I-S154D-A181 D-F183R-Q200L-Y203W-L256E, where the numbering refers to the numbering according to SEQ ID NO:1 .

[0076] In preferred embodiments, the agent according to the invention comprises

[0077] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three, preferably four of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions, preferably four amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least one and increasingly preferably two, three, four, five or six of the positions corresponding to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209,212 or 256, at least one and increasingly preferably two, three, four, five or six amino acid substitutions selected from the group consisting of N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G, Y161T, A163G, V171L, A181D, F183R, Q185R, Q200A / L / S / T, Y203KA / / W, A209K / W, N212S / T and L256D / E / Q, preferably S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0078] (B) at least one compound of formula (I) or (II) where R is each selected from -COOH, Ci-e-alkyl-substituted or unsubstituted C2-6-dicarboxylic acids, Ci-e-alkyl-substituted or unsubstituted C2-e-carboxylic acids and -OOC-NR 2 2, where R 2 are the same or different and selected from Ci-e-alkyl or H, and salts, esters or derivatives thereof, and

[0079] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0080] In further preferred embodiments, the agent according to the invention comprises

[0081] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least one of the positions corresponding to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209, 212 or 256, at least one amino acid substitution selected from the group consisting of N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G, Y161T, A163G,V171 L, A181 D, F183R, Q185R, Q200A / L / S / T, Y203KA / / W, A209K / W, N212S / T and L256D / E / Q, preferably S154D, S160G, A181 D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0082] (B) at least one compound of formula (I) or (II) where R is each selected from -COOH, Ci-e-alkyl-substituted or unsubstituted C2-6-dicarboxylic acids, Ci-e-alkyl-substituted or unsubstituted C2-e-carboxylic acids and -OOC-NR 2 2, where R 2 are the same or different and selected from Ci-e-alkyl or H, and salts, esters or derivatives thereof, and

[0083] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0084] In further preferred embodiments, the agent according to the invention comprises

[0085] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least one of the positions corresponding to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209, 212 or 256, at least one amino acid substitution selected from the group consisting of N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G, Y161T, A163G, V171 L, A181 D, F183R, Q185R, Q200A / L / S / T, Y203K / V / W, A209K / W,N212S / T and L256D / E / Q, preferably,

[0086] S154D, S160G, A181 D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0087] (B) at least one compound of formula (I) or (II) where R is each selected from -COOH, Ci-e-alkyl-substituted or unsubstituted C2-6-dicarboxylic acids, Ci-e-alkyl-substituted or unsubstituted C2-e-carboxylic acids and -OOC-NR 2 2, where R 2 are the same or different and selected from Ci-e-alkyl or H, and salts, esters or derivatives thereof, and

[0088] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0089] In further preferred embodiments, the agent according to the invention comprises

[0090] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least one of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least one amino acid substitution selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0091] (B) at least one compound of formula (I) or (II) where R is each selected from -COOH, Ci-e-alkyl-substituted or unsubstituted C2-6-dicarboxylic acids, Ci-e-alkyl-substituted or unsubstituted C2-e-carboxylic acids and -OOC-NR 2 2, where R 2 are the same or different and selected from Ci-e-alkyl or H, and salts, esters or derivatives thereof, and

[0092] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0093] In further preferred embodiments, the agent according to the invention comprises

[0094] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least two of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least two amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0095] (B) at least one compound of formula (I) or (II) where R is each selected from -COOH, Ci-e-alkyl-substituted or unsubstituted C2-6-dicarboxylic acids, Ci-e-alkyl-substituted or unsubstituted C2-e-carboxylic acids and -OOC-NR 2 2, where R 2 are the same or different and selected from Ci-e-alkyl or H, and salts, esters or derivatives thereof, and

[0096] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0097] In further preferred embodiments, the agent (A) according to the invention comprises a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length to at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least three of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least three amino acid substitutions selected from the group consisting of S154D, S160G,A181 D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0098] (B) at least one compound of formula (I) or (II) where R is each selected from -COOH, Ci-e-alkyl-substituted or unsubstituted C2-6-dicarboxylic acids, Ci-e-alkyl-substituted or unsubstituted C2-e-carboxylic acids and -OOC-NR 2 2, where R 2 are the same or different and selected from Ci-e-alkyl or H, and salts, esters or derivatives thereof, and

[0099] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0100] In further preferred embodiments, the agent according to the invention comprises

[0101] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least four of the positions corresponding to positions 154, 160, 181, 183,

[0102] 185, 200, 203, 209 or 256, has at least four amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0103] (B) at least one compound of formula (I) or (II) where R is each selected from -COOH, Ci-e-alkyl-substituted or unsubstituted C2-6-dicarboxylic acids, Ci-e-alkyl-substituted or unsubstituted C2-e-carboxylic acids and -OOC-NR 2 2, where R 2 are the same or different and selected from Ci-e-alkyl or H, and salts, esters or derivatives thereof, and

[0104] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0105] In further preferred embodiments, the agent according to the invention comprises

[0106] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least five of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least five amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0107] (B) at least one compound of formula (I) or (II) where R is each selected from -COOH, Ci-e-alkyl-substituted or unsubstituted C2-6-dicarboxylic acids, Ci-e-alkyl-substituted or unsubstituted C2-e-carboxylic acids and -OOC-NR 2 2, where R 2 are the same or different and selected from Ci-e-alkyl or H, and salts, esters or derivatives thereof, and

[0108] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0109] In further preferred embodiments, the agent according to the invention comprises

[0110] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least six of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least six amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0111] (B) at least one compound of formula (I) or (II) where R is each selected from -COOH, Ci-e-alkyl-substituted or unsubstituted C2-6-dicarboxylic acids, Ci-e-alkyl-substituted or unsubstituted C2-e-carboxylic acids and -OOC-NR 2 2, where R 2 are the same or different and selected from Ci-e-alkyl or H, and salts, esters or derivatives thereof, and

[0112] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0113] In further preferred embodiments, the agent according to the invention comprises

[0114] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least one of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least one amino acid substitution selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0115] (B) at least one compound of formula (I) or (II) where R is each selected from -COOH, Ci-e-alkyl-substituted or unsubstituted C2-6-dicarboxylic acids, Ci-e-alkyl-substituted or unsubstituted C2-e-carboxylic acids and -OOC-NR 2 2, where R 2 are the same or different and selected from Ci-e-alkyl or H, and salts, esters or derivatives thereof, and

[0116] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0117] In further preferred embodiments, the agent according to the invention comprises

[0118] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least two of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least two amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0119] (B) at least one compound of formula (I) or (II) where R is each selected from -COOH, Ci-e-alkyl-substituted or unsubstituted C2-6-dicarboxylic acids, Ci-e-alkyl-substituted or unsubstituted C2-e-carboxylic acids and -OOC-NR 2 2, where R 2 are the same or different and selected from Ci-e-alkyl or H, and salts, esters or derivatives thereof, and

[0120] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0121] In further preferred embodiments, the agent according to the invention comprises

[0122] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least three of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least three amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0123] (B) at least one compound of formula (I) or (II) where R is each selected from -COOH, Ci-e-alkyl-substituted or unsubstituted C2-6-dicarboxylic acids, Ci-e-alkyl-substituted or unsubstituted C2-e-carboxylic acids and -OOC-NR 2 2, where R 2 are the same or different and are selected from Ci-e-alkyl or H, and salts, esters or derivatives thereof, and (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0124] In further preferred embodiments, the agent according to the invention comprises

[0125] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least four of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least four amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0126] (B) at least one compound of formula (I) or (II) where R is each selected from -COOH, Ci-e-alkyl-substituted or unsubstituted C2-6-dicarboxylic acids, Ci-e-alkyl-substituted or unsubstituted C2-e-carboxylic acids and -OOC-NR 2 2, where R 2 are the same or different and selected from Ci-e-alkyl or H, and salts, esters or derivatives thereof, and

[0127] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0128] In further preferred embodiments, the agent according to the invention comprises

[0129] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least five of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least five amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0130] (B) at least one compound of formula (I) or (II) where R is each selected from -COOH, Ci-e-alkyl-substituted or unsubstituted C2-6-dicarboxylic acids, Ci-e-alkyl-substituted or unsubstituted C2-e-carboxylic acids and -OOC-NR 2 2, where R 2 are the same or different and selected from Ci-e-alkyl or H, and salts, esters or derivatives thereof, and

[0131] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0132] In further preferred embodiments, the agent according to the invention comprises

[0133] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least six of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least six amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0134] (B) at least one compound of formula (I) or (II) where R is each selected from -COOH, Ci-e-alkyl-substituted or unsubstituted C2-6-dicarboxylic acids, Ci-e-alkyl-substituted or unsubstituted C2-e-carboxylic acids and -OOC-NR 2 2, where R 2 are the same or different and selected from Ci-e-alkyl or H, and salts, esters or derivatives thereof, and

[0135] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0136] In further preferred embodiments, the agent according to the invention comprises

[0137] (A) a protease having one of the following amino acid substitution variants: (i) S3T-V4I-R99E-V199I-Q200L-Y203W; (ii) S3T-V4I-R99E-V199I-N212S; (iii) S3T-V4I-R99E-V199I-N74D; (iv) S3T-V4I-R99E-V199I-S154D-L256E; (v) S3T-V4I-R99E-V199I-Q200L-Y203W-S154D-L256E; (vi) S3T-V4I-R99E-V199I-N74D-Q200L-Y203W; (vii) S3T-V4I-R99E-V199I-N74D-S154D-Q200L-Y203W-L256E; (viii) S3T-V4I-R99E-V199I-N74D-N212S; (ix) S3T-V4I-R99E-V199I-N74D-S154D-Y203W-L256E; (x) S3T-V4I-R99E-V199I-N74D-Y203W; (xi) S3T-V4I-R99E-V199I-N74D-S154D-Q200L-L256E; (xii) S3T-V4I-R99E-V199I-N74D-Q200L; (xiii) S3T-V4I-R99E-V199I-S154D-Q200L-Y203W; (xiv) S3T-V4I-R99E-V199I-Q200L-Y203W-L256E; (xv) S3T-V4I-R99E-V199I-A136Q-R143W-Y161T-Q200L; (xvi) S3T-V4I-R99E-V199I-N74D-R143Y-A209W-N212S-L256E; (xvii) S3T-V4I-R99E-V199I-A136Q-S154D-V171 L-Q200L; (xviii) S3T-V4I-R99E-V199I-Q200L-Y203W-A209K-S154D-L256E; (xix) S3T-V4I-R99E-V199I-S154D-S160G-Q185R-Q200L-Y203W-L256E;(xx) S3T-V4I-R99E-V199I-S1540- Al 81 D-F183R-Q200L-Y203W-L256E, wherein the numbering is in each case related to the numbering according to SEQ ID NO:1;

[0138] (B) at least one compound of formula (I) or (II) where R is each selected from -COOH, Ci-e-alkyl-substituted or unsubstituted C2-6-dicarboxylic acids, Ci-e-alkyl-substituted or unsubstituted C2-e-carboxylic acids and -OOC-NR 2 2, where R 2 are the same or different and selected from Ci-e-alkyl or H, and salts, esters or derivatives thereof, and

[0139] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0140] In preferred embodiments, the agent according to the invention comprises

[0141] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three, preferably four of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions, preferably four amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least one and increasingly preferably two, three, four, five or six of the positions corresponding to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209,212 or 256, at least one and increasingly preferably two, three, four, five or six amino acid substitutions selected from the group consisting of N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G, Y161T, A163G, V171L, A181D, F183R, Q185R, Q200A / L / S / T, Y203KA / / W, A209K / W, N212S / T and L256D / E / Q, preferably S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0142] (B) a compound selected from the group consisting of benzoic acid, phenylmalonic acid, benzylmalonic acid, phenylsuccinic acid, benzylsuccinic acid, methyl 3-benzoylpropionate, (S)-3-phenylbutyric acid and benzylcarbamate, and

[0143] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0144] In further preferred embodiments, the agent (A) according to the invention comprises a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length to at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least one of the positions corresponding to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209, 212 or 256, at least one amino acid substitution,which are selected from the group consisting of N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G, Y161T, A163G, V171L, A181D, F183R, Q185R, Q200A / L / S / T, Y203KA / / W, A209K / W, N212S / T and L256D / E / Q, preferably S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0145] (B) a compound selected from the group consisting of benzoic acid, phenylmalonic acid, benzylmalonic acid, phenylsuccinic acid, benzylsuccinic acid, methyl 3-benzoylpropionate, (S)-3-phenylbutyric acid and benzylcarbamate, and

[0146] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0147] In further preferred embodiments, the agent according to the invention comprises

[0148] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least one of the positions corresponding to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209, 212 or 256, at least one amino acid substitution selected from the group consisting of N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G, Y161T, A163G, V171 L, A181 D, F183R, Q185R, Q200A / L / S / T, Y203KA / / W, A209K / W,N212S / T and L256D / E / Q, preferably S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0149] (B) a compound selected from the group consisting of benzoic acid, phenylmalonic acid, benzylmalonic acid, phenylsuccinic acid, benzylsuccinic acid, methyl 3-benzoylpropionate, (S)-3-phenylbutyric acid and benzylcarbamate, and

[0150] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0151] In further preferred embodiments, the agent (A) according to the invention comprises a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length to at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least one of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least one amino acid substitution selected from the group consisting of S154D, S160G,A181 D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0152] (B) a compound selected from the group consisting of benzoic acid, phenylmalonic acid, benzylmalonic acid, phenylsuccinic acid, benzylsuccinic acid, methyl 3-benzoylpropionate, (S)-3-phenylbutyric acid and benzylcarbamate, and

[0153] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0154] In further preferred embodiments, the agent according to the invention comprises

[0155] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least two of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least two amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0156] (B) a compound selected from the group consisting of benzoic acid, phenylmalonic acid, benzylmalonic acid, phenylsuccinic acid, benzylsuccinic acid, methyl 3-benzoylpropionate, (S)-3-phenylbutyric acid and benzylcarbamate, and

[0157] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0158] In further preferred embodiments, the agent according to the invention comprises

[0159] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least three of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least three amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0160] (B) a compound selected from the group consisting of benzoic acid, phenylmalonic acid, benzylmalonic acid, phenylsuccinic acid, benzylsuccinic acid, methyl 3-benzoylpropionate, (S)-3-phenylbutyric acid and benzylcarbamate, and

[0161] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0162] In further preferred embodiments, the agent according to the invention comprises

[0163] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least four of the positions corresponding to positions 154, 160, 181, 183,

[0164] 185, 200, 203, 209 or 256, has at least four amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0165] (B) a compound selected from the group consisting of benzoic acid, phenylmalonic acid, benzylmalonic acid, phenylsuccinic acid, benzylsuccinic acid, methyl 3-benzoylpropionate, (S)-3-phenylbutyric acid and benzylcarbamate, and

[0166] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0167] In further preferred embodiments, the agent according to the invention comprises

[0168] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least five of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least five amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0169] (B) a compound selected from the group consisting of benzoic acid, phenylmalonic acid, benzylmalonic acid, phenylsuccinic acid, benzylsuccinic acid, methyl 3-benzoylpropionate, (S)-3-phenylbutyric acid and benzylcarbamate, and

[0170] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0171] In further preferred embodiments, the agent according to the invention comprises

[0172] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least six of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least six amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0173] (B) a compound selected from the group consisting of benzoic acid, phenylmalonic acid, benzylmalonic acid, phenylsuccinic acid, benzylsuccinic acid, methyl 3-benzoylpropionate, (S)-3-phenylbutyric acid and benzylcarbamate, and

[0174] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0175] In further preferred embodiments, the agent according to the invention comprises

[0176] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least one of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least one amino acid substitution selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0177] (B) a compound selected from the group consisting of benzoic acid, phenylmalonic acid, benzylmalonic acid, phenylsuccinic acid, benzylsuccinic acid, methyl 3-benzoylpropionate, (S)-3-phenylbutyric acid and benzylcarbamate, and

[0178] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0179] In further preferred embodiments, the agent according to the invention comprises

[0180] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least two of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least two amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0181] (B) a compound selected from the group consisting of benzoic acid, phenylmalonic acid, benzylmalonic acid, phenylsuccinic acid, benzylsuccinic acid, methyl 3-benzoylpropionate, (S)-3-phenylbutyric acid and benzylcarbamate, and

[0182] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0183] In further preferred embodiments, the agent according to the invention comprises

[0184] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least three of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least three amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0185] (B) a compound selected from the group consisting of benzoic acid, phenylmalonic acid, benzylmalonic acid, phenylsuccinic acid, benzylsuccinic acid, methyl 3-benzoylpropionate, (S)-3-phenylbutyric acid and benzylcarbamate, and (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0186] In further preferred embodiments, the agent according to the invention comprises

[0187] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least four of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least four amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0188] (B) a compound selected from the group consisting of benzoic acid, phenylmalonic acid, benzylmalonic acid, phenylsuccinic acid, benzylsuccinic acid, methyl 3-benzoylpropionate, (S)-3-phenylbutyric acid and benzylcarbamate, and

[0189] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0190] In further preferred embodiments, the agent according to the invention comprises

[0191] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least five of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least five amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0192] (B) a compound selected from the group consisting of benzoic acid, phenylmalonic acid, benzylmalonic acid, phenylsuccinic acid, benzylsuccinic acid, methyl 3-benzoylpropionate, (S)-3-phenylbutyric acid and benzylcarbamate, and

[0193] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0194] In further preferred embodiments, the agent (A) according to the invention comprises a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length to at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least six of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least six amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0195] (B) a compound selected from the group consisting of benzoic acid, phenylmalonic acid, benzylmalonic acid, phenylsuccinic acid, benzylsuccinic acid, methyl 3-benzoylpropionate, (S)-3-phenylbutyric acid and benzylcarbamate, and

[0196] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0197] In further preferred embodiments, the agent according to the invention comprises

[0198] (A) a protease having one of the following amino acid substitution variants: (i) S3T-V4I-R99E-V199I-Q200L-Y203W; (ii) S3T-V4I-R99E-V199I-N212S; (iii) S3T-V4I-R99E-V199I-N74D; (iv) S3T-V4I-R99E-V199I-S154D-L256E; (v) S3T-V4I-R99E-V199I-Q200L-Y203W-S154D-L256E; (vi) S3T-V4I-R99E-V199I-N74D-Q200L-Y203W; (vii) S3T-V4I-R99E-V199I-N74D-S154D-Q200L-Y203W-L256E; (viii) S3T-V4I-R99E-V199I-N74D-N212S; (ix) S3T-V4I-R99E-V199I-N74D-S154D-Y203W-L256E; (x) S3T-V4I-R99E-V199I-N74D-Y203W; (xi) S3T-V4I-R99E-V199I-N74D-S154D-Q200L-L256E; (xii) S3T-V4I-R99E-V199I-N74D-Q200L; (xiii) S3T-V4I-R99E-V199I-S154D-Q200L-Y203W; (xiv) S3T-V4I-R99E-V199I-Q200L-Y203W-L256E; (xv) S3T-V4I-R99E-V199I-A136Q-R143W-Y161T-Q200L; (xvi) S3T-V4I-R99E-V199I-N74D-R143Y-A209W-N212S-L256E; (xvii) S3T-V4I-R99E-V199I-A136Q-S154D-V171 L-Q200L; (xviii) S3T-V4I-R99E-V199I-Q200L-Y203W-A209K-S154D-L256E; (xix) S3T-V4I-R99E-V199I-S154D-S160G-Q185R-Q200L-Y203W-L256E;(xx) S3T-V4I-R99E-V199I-S154D- A181 D-F183R-Q200L-Y203W-L256E, where the numbering is based on the numbering according to SEQ ID NO:1;

[0199] (B) a compound selected from the group consisting of benzoic acid, phenylmalonic acid, benzylmalonic acid, phenylsuccinic acid, benzylsuccinic acid, methyl 3-benzoylpropionate, (S)-3-phenylbutyric acid and benzylcarbamate, and

[0200] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0201] In preferred embodiments, the agent according to the invention comprises

[0202] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three, preferably four of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions, preferably four amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least one and increasingly preferably two, three, four, five or six of the positions corresponding to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209,212 or 256, at least one and increasingly preferably two, three, four, five or six amino acid substitutions selected from the group consisting of N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G, Y161T, A163G, V171L, A181D, F183R, Q185R, Q200A / L / S / T, Y203KA / / W, A209K / W, N212S / T and L256D / E / Q, preferably S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0203] (B) benzoic acid, and

[0204] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0205] In further preferred embodiments, the agent according to the invention comprises

[0206] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least one of the positions corresponding to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209, 212 or 256, at least one amino acid substitution selected from the group consisting of N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G, Y161T, A163G,V171 L, A181 D, F183R, Q185R, Q200A / L / S / T, Y203KA / / W, A209K / W, N212S / T and L256D / E / Q, preferably S154D, S160G, A181 D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0207] (B) benzoic acid, and

[0208] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0209] In further preferred embodiments, the agent according to the invention comprises

[0210] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least one of the positions corresponding to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209, 212 or 256, at least one amino acid substitution selected from the group consisting of N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G, Y161T, A163G, V171 L, A181 D, F183R, Q185R, Q200A / L / S / T, Y203KA / / W, A209K / W,N212S / T and L256D / E / Q, preferably S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0211] (B) benzoic acid, and

[0212] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0213] In further preferred embodiments, the agent according to the invention comprises

[0214] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least one of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least one amino acid substitution selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0215] (B) benzoic acid, and

[0216] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0217] In further preferred embodiments, the agent according to the invention comprises

[0218] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least two of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least two amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0219] (B) benzoic acid, and

[0220] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0221] In further preferred embodiments, the agent according to the invention comprises

[0222] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least three of the positions corresponding to positions 154, 160, 181, 183,

[0223] 185, 200, 203, 209 or 256, has at least three amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0224] (B) benzoic acid, and

[0225] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0226] In further preferred embodiments, the agent according to the invention comprises

[0227] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least four of the positions corresponding to positions 154, 160, 181, 183,

[0228] 185, 200, 203, 209 or 256, has at least four amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0229] (B) benzoic acid, and

[0230] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase. In further preferred embodiments, the agent according to the invention comprises

[0231] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least five of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least five amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0232] (B) benzoic acid, and

[0233] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0234] In further preferred embodiments, the agent according to the invention comprises

[0235] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least six of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least six amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0236] (B) benzoic acid, and

[0237] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0238] In further preferred embodiments, the agent according to the invention comprises

[0239] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least one of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least one amino acid substitution selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0240] (B) benzoic acid, and

[0241] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0242] In further preferred embodiments, the agent according to the invention comprises

[0243] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least two of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least two amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0244] (B) benzoic acid, and

[0245] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0246] In further preferred embodiments, the agent according to the invention comprises

[0247] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least three of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least three amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0248] (B) benzoic acid, and

[0249] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase. In further preferred embodiments, the agent according to the invention comprises

[0250] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least four of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least four amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0251] (B) benzoic acid, and

[0252] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0253] In further preferred embodiments, the agent according to the invention comprises

[0254] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least five of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least five amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0255] (B) benzoic acid, and

[0256] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0257] In further preferred embodiments, the agent according to the invention comprises

[0258] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least six of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least six amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0259] (B) benzoic acid, and

[0260] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0261] In further preferred embodiments, the agent according to the invention comprises

[0262] (A) a protease having one of the following amino acid substitution variants: (i) S3T-V4I-R99E-V199I-Q200L-Y203W; (ii) S3T-V4I-R99E-V199I-N212S; (iii) S3T-V4I-R99E-V199I-N74D; (iv) S3T-V4I-R99E-V199I-S154D-L256E; (v) S3T-V4I-R99E-V199I-Q200L-Y203W-S154D-L256E; (vi) S3T-V4I-R99E-V199I-N74D-Q200L-Y203W; (vii) S3T-V4I-R99E-V199I-N74D-S154D-Q200L-Y203W-L256E; (viii) S3T-V4I-R99E-V199I-N74D-N212S; (ix) S3T-V4I-R99E-V199I-N74D-S154D-Y203W-L256E; (x) S3T-V4I-R99E-V199I-N74D-Y203W; (xi) S3T-V4I-R99E-V199I-N74D-S154D-Q200L-L256E; (xii) S3T-V4I-R99E-V199I-N74D-Q200L; (xiii) S3T-V4I-R99E-V199I-S154D-Q200L-Y203W; (xiv) S3T-V4I-R99E-V199I-Q200L-Y203W-L256E; (xv) S3T-V4I-R99E-V199I-A136Q-R143W-Y161T-Q200L; (xvi) S3T-V4I-R99E-V199I-N74D-R143Y-A209W-N212S-L256E; (xvii) S3T-V4I-R99E-V199I-A136Q-S154D-V171 L-Q200L; (xviii) S3T-V4I-R99E-V199I-Q200L-Y203W-A209K-S154D-L256E; (xix) S3T-V4I-R99E-V199I-S154D-S160G-Q185R-Q200L-Y203W-L256E;(xx) S3T-V4I-R99E-V199I-S154D- A181 D-F183R-Q200L-Y203W-L256E, where the numbering is based on the numbering according to SEQ ID NO:1;

[0263] (B) benzoic acid, and

[0264] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0265] In preferred embodiments, the agent according to the invention comprises

[0266] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three, preferably four of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions, preferably four amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least one and increasingly preferably two, three, four, five or six of the positions corresponding to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209,212 or 256, at least one and increasingly preferably two, three, four, five or six amino acid substitutions selected from the group consisting of N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G, Y161T, A163G, V171L, A181D, F183R, Q185R, Q200A / L / S / T, Y203KA / / W, A209K / W, N212S / T and L256D / E / Q, preferably S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0267] (B) phenylmalonic acid, and

[0268] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0269] In further preferred embodiments, the agent according to the invention comprises

[0270] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least one of the positions corresponding to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209, 212 or 256, at least one amino acid substitution selected from the group consisting of N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G, Y161T, A163G,V171 L, A181 D, F183R, Q185R, Q200A / L / S / T, Y203KA / / W, A209K / W, N212S / T and L256D / E / Q, preferably S154D, S160G, A181 D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0271] (B) phenylmalonic acid, and

[0272] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0273] In further preferred embodiments, the agent according to the invention comprises

[0274] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least one of the positions corresponding to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209, 212 or 256, at least one amino acid substitution selected from the group consisting of N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G, Y161T, A163G, V171 L, A181 D, F183R, Q185R, Q200A / L / S / T, Y203KA / / W, A209K / W,N212S / T and L256D / E / Q, preferably S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0275] (B) phenylmalonic acid, and (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0276] In further preferred embodiments, the agent according to the invention comprises

[0277] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least one of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least one amino acid substitution selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0278] (B) phenylmalonic acid, and

[0279] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0280] In further preferred embodiments, the agent according to the invention comprises

[0281] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least two of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least two amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0282] (B) phenylmalonic acid, and

[0283] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0284] In further preferred embodiments, the agent according to the invention comprises

[0285] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least three of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least three amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0286] (B) phenylmalonic acid, and

[0287] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0288] In further preferred embodiments, the agent according to the invention comprises

[0289] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least four of the positions corresponding to positions 154, 160, 181, 183,

[0290] 185, 200, 203, 209 or 256, has at least four amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0291] (B) phenylmalonic acid, and

[0292] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0293] In further preferred embodiments, the agent according to the invention comprises

[0294] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least five of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least five amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0295] (B) phenylmalonic acid, and

[0296] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0297] In further preferred embodiments, the agent according to the invention comprises

[0298] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least six of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least six amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0299] (B) phenylmalonic acid, and

[0300] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0301] In further preferred embodiments, the agent according to the invention comprises

[0302] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least one of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least one amino acid substitution selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0303] (B) phenylmalonic acid, and

[0304] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase. In further preferred embodiments, the agent according to the invention comprises

[0305] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least two of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least two amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0306] (B) phenylmalonic acid, and

[0307] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0308] In further preferred embodiments, the agent according to the invention comprises

[0309] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least three of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least three amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0310] (B) phenylmalonic acid, and

[0311] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0312] In further preferred embodiments, the agent according to the invention comprises

[0313] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least four of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least four amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0314] (B) phenylmalonic acid, and

[0315] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0316] In further preferred embodiments, the agent according to the invention comprises

[0317] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least five of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least five amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0318] (B) phenylmalonic acid, and

[0319] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0320] In further preferred embodiments, the agent according to the invention comprises

[0321] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least six of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least six amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0322] (B) phenylmalonic acid, and

[0323] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0324] In further preferred embodiments, the agent (A) according to the invention comprises a protease having one of the following amino acid substitution variants: (i) S3T-V4I-R99E-V199I-Q200L-Y203W; (ii) S3T-V4I-R99E-V199I-N212S; (iii) S3T-V4I-R99E-V199I-N74D; (iv) S3T-V4I-R99E-V199I-S154D-L256E; (v) S3T-V4I-R99E-V199I-Q200L-Y203W-S154D-L256E; (vi) S3T-V4I-R99E-V199I-N74D-Q200L-Y203W; (vii) S3T-V4I-R99E-V199I-N74D-S154D-Q200L-Y203W-L256E; (viii) S3T-V4I-R99E-V199I-N74D-N212S; (ix) S3T-V4I-R99E-V199I-N74D-S154D-Y203W-L256E; (x) S3T-V4I-R99E-V199I-N74D-Y203W; (xi) S3T-V4I-R99E-V199I-N74D-S154D-Q200L-L256E; (xii) S3T-V4I-R99E-V199I-N74D-Q200L; (xiii) S3T-V4I-R99E-V199I-S154D-Q200L-Y203W; (xiv) S3T-V4I-R99E-V199I-Q200L-Y203W-L256E; (xv) S3T-V4I-R99E-V199I-A136Q-R143W-Y161T-Q200L; (xvi) S3T-V4I-R99E-V199I-N74D-R143Y-A209W-N212S-L256E; (xvii) S3T-V4I-R99E-V199I-A136Q-S154D-V171 L-Q200L; (xviii) S3T-V4I-R99E-V199I-Q200L-Y203W-A209K-S154D-L256E;(xix) S3T-V4I-R99E-V199I-S154D-S160G-Q185R-Q200L-Y203W-L256E; (xx) S3T-V4I-R99E-V199I-S154D-A181D-F183R-Q200L-Y203W-L256E, where the numbering is based on the numbering according to SEQ ID NO:1;

[0325] (B) phenylmalonic acid, and

[0326] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0327] In preferred embodiments, the agent according to the invention comprises

[0328] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three, preferably four of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions, preferably four amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least one and increasingly preferably two, three, four, five or six of the positions corresponding to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209,212 or 256, at least one and increasingly preferably two, three, four, five or six amino acid substitutions selected from the group consisting of N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G, Y161T, A163G, V171L, A181D, F183R, Q185R, Q200A / L / S / T, Y203KA / / W, A209K / W, N212S / T and L256D / E / Q, preferably S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0329] (B) Benzylmalonic acid, and

[0330] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0331] In further preferred embodiments, the agent according to the invention comprises

[0332] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least one of the positions corresponding to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209, 212 or 256, at least one amino acid substitution selected from the group consisting of N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G, Y161T, A163G,V171 L, A181 D, F183R, Q185R, Q200A / L / S / T, Y203KA / / W, A209K / W, N212S / T and L256D / E / Q, preferably S154D, S160G, A181 D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0333] (B) Benzylmalonic acid, and

[0334] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0335] In further preferred embodiments, the agent according to the invention comprises

[0336] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least one of the positions corresponding to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209, 212 or 256, at least one amino acid substitution selected from the group consisting of N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G, Y161T, A163G, V171 L, A181 D, F183R, Q185R, Q200A / L / S / T, Y203KA / / W, A209K / W,N212S / T and L256D / E / Q, preferably S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0337] (B) Benzylmalonic acid, and

[0338] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0339] In further preferred embodiments, the agent according to the invention comprises

[0340] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least one of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least one amino acid substitution selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0341] (B) Benzylmalonic acid, and

[0342] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0343] In further preferred embodiments, the agent according to the invention comprises

[0344] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least two of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least two amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0345] (B) Benzylmalonic acid, and

[0346] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0347] In further preferred embodiments, the agent according to the invention comprises

[0348] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least three of the positions corresponding to positions 154, 160, 181, 183,

[0349] 185, 200, 203, 209 or 256, has at least three amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0350] (B) benzylmalonic acid, and (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0351] In further preferred embodiments, the agent according to the invention comprises

[0352] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least four of the positions corresponding to positions 154, 160, 181, 183,

[0353] 185, 200, 203, 209 or 256, has at least four amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0354] (B) Benzylmalonic acid, and

[0355] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0356] In further preferred embodiments, the agent according to the invention comprises

[0357] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least five of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least five amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0358] (B) Benzylmalonic acid, and

[0359] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0360] In further preferred embodiments, the agent according to the invention comprises

[0361] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least six of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least six amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0362] (B) Benzylmalonic acid, and

[0363] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0364] In further preferred embodiments, the agent according to the invention comprises

[0365] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least one of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least one amino acid substitution selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0366] (B) Benzylmalonic acid, and

[0367] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0368] In further preferred embodiments, the agent according to the invention comprises

[0369] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least two of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least two amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0370] (B) Benzylmalonic acid, and

[0371] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0372] In further preferred embodiments, the agent according to the invention comprises

[0373] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least three of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least three amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0374] (B) Benzylmalonic acid, and

[0375] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0376] In further preferred embodiments, the agent according to the invention comprises

[0377] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least four of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least four amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0378] (B) Benzylmalonic acid, and

[0379] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0380] In further preferred embodiments, the agent (A) according to the invention comprises a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length to at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least five of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least five amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0381] (B) Benzylmalonic acid, and

[0382] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0383] In further preferred embodiments, the agent according to the invention comprises

[0384] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least six of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least six amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0385] (B) Benzylmalonic acid, and

[0386] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0387] In further preferred embodiments, the agent according to the invention comprises

[0388] (A) a protease having one of the following amino acid substitution variants: (i) S3T-V4I-R99E-V199I-Q200L-Y203W; (ii) S3T-V4I-R99E-V199I-N212S; (iii) S3T-V4I-R99E-V199I-N74D; (iv) S3T-V4I-R99E-V199I-S154D-L256E; (v) S3T-V4I-R99E-V199I-Q200L-Y203W-S154D-L256E; (vi) S3T-V4I-R99E-V199I-N74D-Q200L-Y203W; (vii) S3T-V4I-R99E-V199I-N74D-S154D-Q200L-Y203W-L256E; (viii) S3T-V4I-R99E-V199I-N74D-N212S; (ix) S3T-V4I-R99E-V199I-N74D-S154D-Y203W-L256E; (x) S3T-V4I-R99E-V199I-N74D-Y203W; (xi) S3T-V4I-R99E-V199I-N74D-S154D-Q200L-L256E; (xii) S3T-V4I-R99E-V199I-N74D-Q200L; (xiii) S3T-V4I-R99E-V199I-S154D-Q200L-Y203W; (xiv) S3T-V4I-R99E-V199I-Q200L-Y203W-L256E; (xv) S3T-V4I-R99E-V199I-A136Q-R143W-Y161T-Q200L; (xvi) S3T-V4I-R99E-V199I-N74D-R143Y-A209W-N212S-L256E; (xvii) S3T-V4I-R99E-V199I-A136Q-S154D-V171 L-Q200L; (xviii) S3T-V4I-R99E-V199I-Q200L-Y203W-A209K-S154D-L256E; (xix) S3T-V4I-R99E-V199I-S154D-S160G-Q185R-Q200L-Y203W-L256E;(xx) S3T-V4I-R99E-V199I-S1540- Al 81 D-F183R-Q200L-Y203W-L256E, wherein the numbering is in each case related to the numbering according to SEQ ID NO:1;

[0389] (B) Benzylmalonic acid, and

[0390] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0391] In preferred embodiments, the agent according to the invention comprises

[0392] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three, preferably four of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions, preferably four amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least one and increasingly preferably two, three, four, five or six of the positions corresponding to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209,212 or 256, at least one and increasingly preferably two, three, four, five or six amino acid substitutions selected from the group consisting of N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G, Y161T, A163G, V171L, A181D, F183R, Q185R, Q200A / L / S / T, Y203KA / / W, A209K / W, N212S / T and L256D / E / Q, preferably S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0393] (B) Phenylsuccinic acid, and

[0394] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0395] In further preferred embodiments, the agent according to the invention comprises

[0396] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least one of the positions corresponding to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209, 212 or 256, at least one amino acid substitution selected from the group consisting of N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G, Y161T, A163G,V171 L, A181 D, F183R, Q185R, Q200A / L / S / T, Y203KA / / W, A209K / W, N212S / T and L256D / E / Q, preferably S154D, S160G, A181 D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0397] (B) Phenylsuccinic acid, and

[0398] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0399] In further preferred embodiments, the agent according to the invention comprises

[0400] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least one of the positions corresponding to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209, 212 or 256, at least one amino acid substitution selected from the group consisting of N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G, Y161T, A163G, V171 L, A181 D, F183R, Q185R, Q200A / L / S / T, Y203KA / / W, A209K / W,N212S / T and L256D / E / Q, preferably S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0401] (B) Phenylsuccinic acid, and

[0402] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0403] In further preferred embodiments, the agent according to the invention comprises

[0404] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least one of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least one amino acid substitution selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0405] (B) phenylsuccinic acid, and (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0406] In further preferred embodiments, the agent according to the invention comprises

[0407] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least two of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least two amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0408] (B) Phenylsuccinic acid, and

[0409] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0410] In further preferred embodiments, the agent according to the invention comprises

[0411] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least three of the positions corresponding to positions 154, 160, 181, 183,

[0412] 185, 200, 203, 209 or 256, has at least three amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0413] (B) Phenylsuccinic acid, and

[0414] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0415] In further preferred embodiments, the agent according to the invention comprises

[0416] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least four of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least four amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0417] (B) phenylsuccinic acid, and

[0418] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0419] In further preferred embodiments, the agent according to the invention comprises

[0420] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least five of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least five amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0421] (B) Phenylsuccinic acid, and

[0422] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0423] In further preferred embodiments, the agent according to the invention comprises

[0424] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least six of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least six amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0425] (B) Phenylsuccinic acid, and

[0426] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0427] In further preferred embodiments, the agent according to the invention comprises

[0428] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least one of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least one amino acid substitution selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0429] (B) phenylsuccinic acid, and

[0430] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0431] In further preferred embodiments, the agent according to the invention comprises

[0432] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least two of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least two amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0433] (B) phenylsuccinic acid, and

[0434] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0435] In further preferred embodiments, the agent (A) according to the invention comprises a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length to at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least three of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least three amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0436] (B) phenylsuccinic acid, and

[0437] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0438] In further preferred embodiments, the agent according to the invention comprises

[0439] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least four of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least four amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0440] (B) phenylsuccinic acid, and

[0441] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0442] In further preferred embodiments, the agent according to the invention comprises

[0443] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least five of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least five amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0444] (B) phenylsuccinic acid, and

[0445] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0446] In further preferred embodiments, the agent according to the invention comprises

[0447] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least six of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least six amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0448] (B) phenylsuccinic acid, and

[0449] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0450] In further preferred embodiments, the agent according to the invention comprises

[0451] (A) a protease having one of the following amino acid substitution variants: (i) S3T-V4I-R99E-V199I-Q200L-Y203W; (ii) S3T-V4I-R99E-V199I-N212S; (iii) S3T-V4I-R99E-V199I-N74D; (iv) S3T-V4I-R99E-V199I-S154D-L256E; (v) S3T-V4I-R99E-V199I-Q200L-Y203W-S154D-L256E; (vi) S3T-V4I-R99E-V199I-N74D-Q200L-Y203W; (vii) S3T-V4I-R99E-V199I-N74D-S154D-Q200L-Y203W-L256E; (viii) S3T-V4I-R99E-V199I-N74D-N212S; (ix) S3T-V4I-R99E-V199I-N74D-S154D-Y203W-L256E; (x) S3T-V4I-R99E-V199I-N74D-Y203W; (xi) S3T-V4I-R99E-V199I-N74D-S154D-Q200L-L256E; (xii) S3T-V4I-R99E-V199I-N74D-Q200L; (xiii) S3T-V4I-R99E-V199I-S154D-Q200L-Y203W; (xiv) S3T-V4I-R99E-V199I-Q200L-Y203W-L256E; (xv) S3T-V4I-R99E-V199I-A136Q-R143W-Y161T-Q200L; (xvi) S3T-V4I-R99E-V199I-N74D-R143Y-A209W-N212S-L256E; (xvii) S3T-V4I-R99E-V199I-A136Q-S154D-V171 L-Q200L; (xviii) S3T-V4I-R99E-V199I-Q200L-Y203W-A209K-S154D-L256E; (xix) S3T-V4I-R99E-V199I-S154D-S160G-Q185R-Q200L-Y203W-L256E;(xx) S3T-V4I-R99E-V199I-S154D- A181 D-F183R-Q200L-Y203W-L256E, where the numbering is based on the numbering according to SEQ ID NO:1;

[0452] (B) Phenylsuccinic acid, and

[0453] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase. In preferred embodiments, the agent according to the invention comprises

[0454] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three, preferably four of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions, preferably four amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least one and increasingly preferably two, three, four, five or six of the positions corresponding to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209,212 or 256, at least one and increasingly preferably two, three, four, five or six amino acid substitutions selected from the group consisting of N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G, Y161T, A163G, V171L, A181D, F183R, Q185R, Q200A / L / S / T, Y203KA / / W, A209K / W, N212S / T and L256D / E / Q, preferably S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0455] (B) Benzylsuccinic acid, and

[0456] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0457] In further preferred embodiments, the agent according to the invention comprises

[0458] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least one of the positions corresponding to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209, 212 or 256, at least one amino acid substitution selected from the group consisting of N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G, Y161T, A163G,V171 L, A181 D, F183R, Q185R, Q200A / L / S / T, Y203KA / / W, A209K / W, N212S / T and L256D / E / Q, preferably S154D, S160G, A181 D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0459] (B) Benzylsuccinic acid, and

[0460] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0461] In further preferred embodiments, the agent (A) according to the invention comprises a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length to at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least one of the positions corresponding to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209, 212 or 256, at least one amino acid substitution selected from the group consisting of N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G, Y161T, A163G,V171 L, A181 D, F183R, Q185R, Q200A / L / S / T, Y203KA / / W, A209K / W, N212S / T and L256D / E / Q, preferably S154D, S160G, A181 D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0462] (B) Benzylsuccinic acid, and

[0463] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0464] In further preferred embodiments, the agent according to the invention comprises

[0465] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least one of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least one amino acid substitution selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0466] (B) Benzylsuccinic acid, and

[0467] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0468] In further preferred embodiments, the agent according to the invention comprises

[0469] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least two of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least two amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0470] (B) Benzylsuccinic acid, and

[0471] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0472] In further preferred embodiments, the agent according to the invention comprises

[0473] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least three of the positions corresponding to positions 154, 160, 181, 183,

[0474] 185, 200, 203, 209 or 256, has at least three amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0475] (B) Benzylsuccinic acid, and

[0476] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0477] In further preferred embodiments, the agent according to the invention comprises

[0478] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least four of the positions corresponding to positions 154, 160, 181, 183,

[0479] 185, 200, 203, 209 or 256, has at least four amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0480] (B) benzylsuccinic acid, and (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0481] In further preferred embodiments, the agent according to the invention comprises

[0482] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least five of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least five amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0483] (B) Benzylsuccinic acid, and

[0484] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0485] In further preferred embodiments, the agent according to the invention comprises

[0486] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least six of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least six amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0487] (B) Benzylsuccinic acid, and

[0488] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0489] In further preferred embodiments, the agent according to the invention comprises

[0490] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least one of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least one amino acid substitution selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0491] (B) Benzylsuccinic acid, and

[0492] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0493] In further preferred embodiments, the agent according to the invention comprises

[0494] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least two of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least two amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0495] (B) Benzylsuccinic acid, and

[0496] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0497] In further preferred embodiments, the agent according to the invention comprises

[0498] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least three of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least three amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E, (B) benzylsuccinic acid, and

[0499] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0500] In further preferred embodiments, the agent according to the invention comprises

[0501] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least four of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least four amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0502] (B) Benzylsuccinic acid, and

[0503] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0504] In further preferred embodiments, the agent according to the invention comprises

[0505] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least five of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least five amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0506] (B) Benzylsuccinic acid, and

[0507] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0508] In further preferred embodiments, the agent according to the invention comprises

[0509] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least six of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least six amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0510] (B) Benzylsuccinic acid, and

[0511] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0512] In further preferred embodiments, the agent according to the invention comprises

[0513] (A) a protease having one of the following amino acid substitution variants: (i) S3T-V4I-R99E-V199I-Q200L-Y203W; (ii) S3T-V4I-R99E-V199I-N212S; (iii) S3T-V4I-R99E-V199I-N74D; (iv) S3T-V4I-R99E-V199I-S154D-L256E; (v) S3T-V4I-R99E-V199I-Q200L-Y203W-S154D-L256E; (vi) S3T-V4I-R99E-V199I-N74D-Q200L-Y203W; (vii) S3T-V4I-R99E-V199I-N74D-S154D-Q200L-Y203W-L256E; (viii) S3T-V4I-R99E-V199I-N74D-N212S; (ix) S3T-V4I-R99E-V199I-N74D-S154D-Y203W-L256E; (x) S3T-V4I-R99E-V199I-N74D-Y203W; (xi) S3T-V4I-R99E-V199I-N74D-S154D-Q200L-L256E; (xii) S3T-V4I-R99E-V199I-N74D-Q200L; (xiii) S3T-V4I-R99E-V199I-S154D-Q200L-Y203W; (xiv) S3T-V4I-R99E-V199I-Q200L-Y203W-L256E; (xv) S3T-V4I-R99E-V199I-A136Q-R143W-Y161T-Q200L; (xvi) S3T-V4I-R99E-V199I-N74D-R143Y-A209W-N212S-L256E; (xvii) S3T-V4I-R99E-V199I-A136Q-S154D-V171 L-Q200L; (xviii) S3T-V4I-R99E-V199I-Q200L-Y203W-A209K-S154D-L256E; (xix) S3T-V4I-R99E-V199I-S154D-S160G-Q185R-Q200L-Y203W-L256E;(xx) S3T-V4I-R99E-V199I-S154D- A181 D-F183R-Q200L-Y203W-L256E, where the numbering is based on the numbering according to SEQ ID NO:1;

[0514] (B) Benzylsuccinic acid, and

[0515] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0516] In preferred embodiments, the agent according to the invention comprises

[0517] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three, preferably four of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions, preferably four amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least one and increasingly preferably two, three, four, five or six of the positions corresponding to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209,212 or 256, at least one and increasingly preferably two, three, four, five or six amino acid substitutions selected from the group consisting of N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G, Y161T, A163G, V171L, A181D, F183R, Q185R, Q200A / L / S / T, Y203KA / / W, A209K / W, N212S / T and L256D / E / Q, preferably S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0518] (B) Methyl 3-benzoylpropionate, and

[0519] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0520] In further preferred embodiments, the agent according to the invention comprises

[0521] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least one of the positions corresponding to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209, 212 or 256, at least one amino acid substitution selected from the group consisting of N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G, Y161T, A163G,V171 L, A181 D, F183R, Q185R, Q200A / L / S / T, Y203KA / / W, A209K / W, N212S / T and L256D / E / Q, preferably S154D, S160G, A181 D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0522] (B) Methyl 3-benzoylpropionate, and

[0523] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0524] In further preferred embodiments, the agent according to the invention comprises

[0525] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least one of the positions corresponding to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209, 212 or 256, at least one amino acid substitution selected from the group consisting of N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G, Y161T, A163G, V171 L, A181 D, F183R, Q185R, Q200A / L / S / T, Y203KA / / W, A209K / W,N212S / T and L256D / E / Q, preferably S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0526] (B) Methyl 3-benzoylpropionate, and

[0527] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0528] In further preferred embodiments, the agent according to the invention comprises

[0529] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least one of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least one amino acid substitution selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0530] (B) Methyl 3-benzoylpropionate, and

[0531] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0532] In further preferred embodiments, the agent according to the invention comprises

[0533] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least two of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least two amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0534] (B) Methyl 3-benzoylpropionate, and

[0535] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase. In further preferred embodiments, the agent according to the invention comprises

[0536] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least three of the positions corresponding to positions 154, 160, 181, 183,

[0537] 185, 200, 203, 209 or 256, has at least three amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0538] (B) Methyl 3-benzoylpropionate, and

[0539] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0540] In further preferred embodiments, the agent according to the invention comprises

[0541] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least four of the positions corresponding to positions 154, 160, 181, 183,

[0542] 185, 200, 203, 209 or 256, has at least four amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0543] (B) Methyl 3-benzoylpropionate, and

[0544] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0545] In further preferred embodiments, the agent according to the invention comprises

[0546] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least five of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least five amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0547] (B) Methyl 3-benzoylpropionate, and

[0548] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0549] In further preferred embodiments, the agent according to the invention comprises

[0550] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least six of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least six amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0551] (B) Methyl 3-benzoylpropionate, and

[0552] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0553] In further preferred embodiments, the agent according to the invention comprises

[0554] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least one of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least one amino acid substitution selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0555] (B) methyl 3-benzoylpropionate, and (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0556] In further preferred embodiments, the agent according to the invention comprises

[0557] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least two of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least two amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0558] (B) Methyl 3-benzoylpropionate, and

[0559] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0560] In further preferred embodiments, the agent according to the invention comprises

[0561] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least three of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least three amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0562] (B) Methyl 3-benzoylpropionate, and

[0563] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0564] In further preferred embodiments, the agent according to the invention comprises

[0565] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least four of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least four amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0566] (B) Methyl 3-benzoylpropionate, and

[0567] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0568] In further preferred embodiments, the agent according to the invention comprises

[0569] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least five of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least five amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0570] B) Methyl 3-benzoylpropionate, and

[0571] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0572] In further preferred embodiments, the agent according to the invention comprises

[0573] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least six of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least six amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0574] (B) methyl 3-benzoylpropionate, and (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0575] In further preferred embodiments, the agent according to the invention comprises

[0576] (A) a protease having one of the following amino acid substitution variants: (i) S3T-V4I-R99E-V199I-Q200L-Y203W; (ii) S3T-V4I-R99E-V199I-N212S; (iii) S3T-V4I-R99E-V199I-N74D; (iv) S3T-V4I-R99E-V199I-S154D-L256E; (v) S3T-V4I-R99E-V199I-Q200L-Y203W-S154D-L256E; (vi) S3T-V4I-R99E-V199I-N74D-Q200L-Y203W; (vii) S3T-V4I-R99E-V199I-N74D-S154D-Q200L-Y203W-L256E; (viii) S3T-V4I-R99E-V199I-N74D-N212S; (ix) S3T-V4I-R99E-V199I-N74D-S154D-Y203W-L256E; (x) S3T-V4I-R99E-V199I-N74D-Y203W; (xi) S3T-V4I-R99E-V199I-N74D-S154D-Q200L-L256E; (xii) S3T-V4I-R99E-V199I-N74D-Q200L; (xiii) S3T-V4I-R99E-V199I-S154D-Q200L-Y203W; (xiv) S3T-V4I-R99E-V199I-Q200L-Y203W-L256E; (xv) S3T-V4I-R99E-V199I-A136Q-R143W-Y161T-Q200L; (xvi) S3T-V4I-R99E-V199I-N74D-R143Y-A209W-N212S-L256E; (xvii) S3T-V4I-R99E-V199I-A136Q-S154D-V171 L-Q200L; (xviii) S3T-V4I-R99E-V199I-Q200L-Y203W-A209K-S154D-L256E; (xix) S3T-V4I-R99E-V199I-S154D-S160G-Q185R-Q200L-Y203W-L256E;(xx) S3T-V4I-R99E-V199I-S154D- A181 D-F183R-Q200L-Y203W-L256E, where the numbering is based on the numbering according to SEQ ID NO:1;

[0577] (B) Methyl 3-benzoylpropionate, and

[0578] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0579] In preferred embodiments, the agent according to the invention comprises

[0580] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three, preferably four of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions, preferably four amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least one and increasingly preferably two, three, four, five or six of the positions corresponding to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209,212 or 256, at least one and increasingly preferably two, three, four, five or six amino acid substitutions selected from the group consisting of N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G, Y161T, A163G, V171L, A181D, F183R, Q185R, Q200A / L / S / T, Y203KA / / W, A209K / W, N212S / T and L256D / E / Q, preferably S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0581] (B) (S)-3-phenylbutyric acid, and (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0582] In further preferred embodiments, the agent according to the invention comprises

[0583] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least one of the positions corresponding to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209, 212 or 256, at least one amino acid substitution selected from the group consisting of N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G, Y161T, A163G,V171 L, A181 D, F183R, Q185R, Q200A / L / S / T, Y203KA / / W, A209K / W, N212S / T and L256D / E / Q, preferably S154D, S160G, A181 D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0584] (B) (S)-3-phenylbutyric acid, and

[0585] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0586] In further preferred embodiments, the agent according to the invention comprises

[0587] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least one of the positions corresponding to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209, 212 or 256, at least one amino acid substitution selected from the group consisting of N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G, Y161T, A163G, V171 L, A181 D, F183R, Q185R, Q200A / L / S / T, Y203KA / / W, A209K / W,N212S / T and L256D / E / Q, preferably S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0588] (B) (S)-3-phenylbutyric acid, and

[0589] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase. In further preferred embodiments, the agent according to the invention comprises

[0590] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least one of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least one amino acid substitution selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0591] (B) (S)-3-phenylbutyric acid, and

[0592] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0593] In further preferred embodiments, the agent according to the invention comprises

[0594] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least two of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least two amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0595] (B) (S)-3-phenylbutyric acid, and

[0596] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0597] In further preferred embodiments, the agent according to the invention comprises

[0598] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least three of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least three amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0599] (B) (S)-3-phenylbutyric acid, and

[0600] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0601] In further preferred embodiments, the agent according to the invention comprises

[0602] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least four of the positions corresponding to positions 154, 160, 181, 183,

[0603] 185, 200, 203, 209 or 256, has at least four amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0604] (B) (S)-3-phenylbutyric acid, and

[0605] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0606] In further preferred embodiments, the agent according to the invention comprises

[0607] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least five of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least five amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0608] (B) (S)-3-phenylbutyric acid, and (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0609] In further preferred embodiments, the agent according to the invention comprises

[0610] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least six of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least six amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0611] (B) (S)-3-phenylbutyric acid, and

[0612] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0613] In further preferred embodiments, the agent according to the invention comprises

[0614] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least one of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least one amino acid substitution selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0615] (B) (S)-3-phenylbutyric acid, and

[0616] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0617] In further preferred embodiments, the agent according to the invention comprises

[0618] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least two of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least two amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0619] (B) (S)-3-phenylbutyric acid, and

[0620] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0621] In further preferred embodiments, the agent according to the invention comprises

[0622] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least three of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least three amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0623] (B) (S)-3-phenylbutyric acid, and

[0624] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0625] In further preferred embodiments, the agent according to the invention comprises

[0626] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least four of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least four amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0627] (B) (S)-3-phenylbutyric acid, and (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0628] In further preferred embodiments, the agent according to the invention comprises

[0629] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least five of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least five amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0630] (B) (S)-3-phenylbutyric acid, and

[0631] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0632] In further preferred embodiments, the agent according to the invention comprises

[0633] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least six of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least six amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0634] (B) (S)-3-phenylbutyric acid, and

[0635] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0636] In further preferred embodiments, the agent according to the invention comprises

[0637] (A) a protease having one of the following amino acid substitution variants: (i) S3T-V4I-R99E-V199I-Q200L-Y203W; (ii) S3T-V4I-R99E-V199I-N212S; (iii) S3T-V4I-R99E-V199I-N74D; (iv) S3T-V4I-R99E-V199I-S154D-L256E; (v) S3T-V4I-R99E-V199I-Q200L-Y203W-S154D-L256E; (vi) S3T-V4I-R99E-V199I-N74D-Q200L-Y203W; (vii) S3T-V4I-R99E-V199I-N74D-S154D-Q200L-Y203W-L256E; (viii) S3T-V4I-R99E-V199I-N74D-N212S; (ix) S3T-V4I-R99E-V199I-N74D-S154D-Y203W-L256E; (x) S3T-V4I-R99E-V199I-N74D-Y203W; (xi) S3T-V4I-R99E-V199I-N74D-S154D-Q200L-L256E; (xii) S3T-V4I-R99E-V199I-N74D-Q200L; (xiii) S3T-V4I-R99E-V199I-S154D-Q200L-Y203W; (xiv) S3T-V4I-R99E-V199I-Q200L-Y203W-L256E; (xv) S3T-V4I-R99E-V199I-A136Q-R143W-Y161T-Q200L; (xvi) S3T-V4I-R99E-V199I-N74D-R143Y-A209W-N212S-L256E; (xvii) S3T-V4I-R99E-V199I-A136Q-S154D-V171 L-Q200L; (xviii) S3T-V4I-R99E-V199I-Q200L-Y203W-A209K-S154D-L256E; (xix) S3T-V4I-R99E-V199I-S154D-S160G-Q185R-Q200L-Y203W-L256E;(xx) S3T-V4I-R99E-V199I-S1540- Al 81 D-F183R-Q200L-Y203W-L256E, wherein the numbering is in each case related to the numbering according to SEQ ID NO:1;

[0638] (B) (S)-3-phenylbutyric acid, and

[0639] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0640] In preferred embodiments, the agent according to the invention comprises

[0641] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three, preferably four of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions, preferably four amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least one and increasingly preferably two, three, four, five or six of the positions corresponding to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209,212 or 256, at least one and increasingly preferably two, three, four, five or six amino acid substitutions selected from the group consisting of N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G, Y161T, A163G, V171L, A181D, F183R, Q185R, Q200A / L / S / T, Y203KA / / W, A209K / W, N212S / T and L256D / E / Q, preferably S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0642] (B) Benzylcarbamate, and

[0643] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0644] In further preferred embodiments, the agent according to the invention comprises

[0645] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least one of the positions corresponding to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209, 212 or 256, at least one amino acid substitution selected from the group consisting of N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G, Y161T, A163G,V171 L, A181 D, F183R, Q185R, Q200A / L / S / T, Y203KA / / W, A209K / W, N212S / T and L256D / E / Q, preferably S154D, S160G, A181 D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0646] (B) Benzylcarbamate, and

[0647] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0648] In further preferred embodiments, the agent according to the invention comprises

[0649] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least one of the positions corresponding to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209, 212 or 256, at least one amino acid substitution selected from the group consisting of N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G, Y161T, A163G, V171 L, A181 D, F183R, Q185R, Q200A / L / S / T, Y203KA / / W, A209K / W,N212S / T and L256D / E / Q, preferably S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0650] (B) Benzylcarbamate, and

[0651] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0652] In further preferred embodiments, the agent according to the invention comprises

[0653] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least one of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least one amino acid substitution selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0654] (B) Benzylcarbamate, and

[0655] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0656] In further preferred embodiments, the agent according to the invention comprises

[0657] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least two of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least two amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0658] (B) Benzylcarbamate, and

[0659] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0660] In further preferred embodiments, the agent according to the invention comprises

[0661] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least three of the positions corresponding to positions 154, 160, 181, 183,

[0662] 185, 200, 203, 209 or 256, has at least three amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0663] (B) Benzylcarbamate, and

[0664] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase. In further preferred embodiments, the agent according to the invention comprises

[0665] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least four of the positions corresponding to positions 154, 160, 181, 183,

[0666] 185, 200, 203, 209 or 256, has at least four amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0667] (B) Benzylcarbamate, and

[0668] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0669] In further preferred embodiments, the agent according to the invention comprises

[0670] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least five of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least five amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0671] (B) Benzylcarbamate, and

[0672] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0673] In further preferred embodiments, the agent according to the invention comprises

[0674] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at least three of the positions corresponding to positions 3, 4, 99 or 199, at least three amino acid substitutions selected from the group consisting of S3T, V4I, R99E and V199I, and (ii) at least six of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least six amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W,A209K and L256E consisting of a group selected,

[0675] (B) Benzylcarbamate, and

[0676] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0677] In further preferred embodiments, the agent according to the invention comprises

[0678] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least one of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least one amino acid substitution selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0679] (B) Benzylcarbamate, and

[0680] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0681] In further preferred embodiments, the agent according to the invention comprises

[0682] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least two of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least two amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0683] (B) benzylcarbamate, and (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0684] In further preferred embodiments, the agent according to the invention comprises

[0685] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least three of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least three amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0686] (B) Benzylcarbamate, and

[0687] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0688] In further preferred embodiments, the agent according to the invention comprises

[0689] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least four of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least four amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0690] (B) Benzylcarbamate, and

[0691] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0692] In further preferred embodiments, the agent according to the invention comprises

[0693] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least five of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least five amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0694] (B) Benzylcarbamate, and

[0695] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0696] In further preferred embodiments, the agent according to the invention comprises

[0697] (A) a protease which has proteolytic activity and comprises an amino acid sequence which corresponds to the amino acid sequence given in SEQ ID NO:1 over its entire length by at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case based on the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I and (ii) at least six of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least six amino acid substitutions selected from the group consisting of S154D, S160G, A181D, F183R, Q185R, Q200L, Y203W, A209K and L256E,

[0698] (B) Benzylcarbamate, and

[0699] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0700] In further preferred embodiments, the agent according to the invention comprises

[0701] (A) a protease having one of the following amino acid substitution variants: (i) S3T-V4I-R99E-V199I-Q200L-Y203W; (ii) S3T-V4I-R99E-V199I-N212S; (iii) S3T-V4I-R99E-V199I-N74D; (iv) S3T-V4I-R99E-V199I-S154D-L256E; (v) S3T-V4I-R99E-V199I-Q200L-Y203W-S154D-L256E; (vi) S3T-V4I-R99E-V199I-N74D-Q200L-Y203W; (vii) S3T-V4I-R99E-V199I-N74D-S154D-Q200L-Y203W-L256E; (viii) S3T-V4I-R99E-V199I-N74D-N212S; (ix) S3T-V4I-R99E-V199I-N74D-S154D-Y203W-L256E; (x) S3T-V4I-R99E-V199I-N74D-Y203W; (xi) S3T-V4I-R99E-V199I-N74D-S154D-Q200L-L256E; (xii) S3T-V4I-R99E-V199I-N74D-Q200L; (xiii) S3T-V4I-R99E-V199I-S154D-Q200L-Y203W; (xiv) S3T-V4I-R99E-V199I-Q200L-Y203W-L256E; (xv) S3T-V4I-R99E-V199I-A136Q-R143W-Y161T-Q200L; (xvi) S3T-V4I-R99E-V199I-N74D-R143Y-A209W-N212S-L256E; (xvii) S3T-V4I-R99E-V199I-A136Q-S154D-V171 L-Q200L; (xviii) S3T-V4I-R99E-V199I-Q200L-Y203W-A209K-S154D-L256E; (xix) S3T-V4I-R99E-V199I-S154D-S160G-Q185R-Q200L-Y203W-L256E;(xx) S3T-V4I-R99E-V199I-S154D-A181D-F183R-Q200L-Y203W-L256E, wherein the numbering is in each case based on the numbering according to SEQ ID NO:1, (B) benzylcarbamate, and;

[0702] (C) at least one further enzyme, wherein the further enzyme is different from the at least one protease and is preferably selected from amylase, cellulase and lipase, preferably cellulase.

[0703] In washing or cleaning agents according to the invention, the washing or cleaning agent comprises, in each case based on the total weight of the washing or cleaning agent, the enzyme, ie the protease, in an amount of 0.005 to 5 wt.%, preferably 0.05 to 2 wt.%, more preferably 0.01 to 0.5 wt.% and even more preferably 0.02 to 0.2 wt.%, and the reversible inhibitor (ie the compound of formula (I) or (II) as defined herein) in an amount of 0.01 to 15 wt.%, preferably 0.05 to 5 wt.%, more preferably 0.1 to 1 wt.%, even more preferably 0.3 to 1.5 wt.% and particularly preferably 0.2 to 0.75 wt.%.

[0704] In various embodiments, the enzyme and the reversible inhibitor can be pre-formulated in an enzyme composition. As can be seen from the previous explanations, the enzyme protein only forms a fraction of the total weight of conventional enzyme preparations. Preferably used protease preparations contain between 0.1 and 40 wt.%, preferably between 0.2 and 30 wt.%, particularly preferably between 0.4 and 20 wt.% and in particular between 0.8 and 10 wt.% of the enzyme protein. In such compositions, the reversible inhibitor can be present in an amount of 0.05 to 35 wt.%, preferably from 0.05 to 10 wt.%, further preferably from 0.1 to 1 wt.%, even more preferably from 0.3 to 1.5 wt.% and particularly preferably from 0.2 to 0.75 wt.%, based on the total weight in the enzyme composition.This enzyme composition, which is also a component of the present invention, can then be used in washing or cleaning agents according to the invention, in amounts which lead to the final concentrations in the washing or cleaning agent stated herein.

[0705] The proteases used according to the invention exhibit enzymatic activity, i.e., they are capable of hydrolyzing peptides and proteins, particularly in detergents or cleaning agents. A protease used according to the invention is therefore an enzyme that catalyzes the hydrolysis of amide / peptide bonds in protein / peptide substrates and is thus capable of cleaving proteins or peptides. Furthermore, a protease used according to the invention is preferably a mature protease, i.e., the catalytically active molecule without signal and / or propeptide(s). Unless otherwise stated, the sequences indicated also refer to mature (processed) enzymes.

[0706] In various embodiments of the invention, the protease is a free enzyme. This means that the protease can interact directly with all components of a composition and, if the composition is a liquid composition, that the protease is in direct contact with the composition's solvent (e.g., water). In other embodiments, a composition may contain proteases that form an interaction complex with other molecules or that contain a "coat." In this case, a single or multiple protease molecules may be separated from the other components of the composition by a surrounding structure. Such a separating structure may be formed by, but is not limited to, vesicles, such as a micelle or a liposome. However, the surrounding structure may also be a virus particle, a bacterial cell, or a eukaryotic cell.In various embodiments, an agent may contain cells of Bacillus pumilus or Bacillus subtilis expressing the proteases of the invention, or cell culture supernatants of such cells.

[0707] In the context of the present invention, the feature that a protease has the specified substitutions means that it contains one (of the specified) substitution(s) at the respective position, i.e., at least the specified positions are not otherwise mutated or deleted, for example, by fragmentation of the protease. In various preferred embodiments, the proteases described herein, with the exception of the explicitly mentioned substitutions, have the sequence of SEQ ID NO:1, i.e., apart from the substituted positions, they are 100% identical to the sequence according to SEQ ID NO:1.

[0708] The identity of nucleic acid or amino acid sequences is determined by sequence comparison. This sequence comparison is based on the BLAST algorithm, which is established and commonly used in the state of the art (see, for example, Altschul et al. (1990) Basic local alignment search tool, J. Mol. Biol., 215:403-410, and Altschul et al. (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs, Nucleic Acids Res., 25:3389-3402) and is essentially achieved by matching similar sequences of nucleotides or amino acids in the nucleic acid or amino acid sequences to one another. A tabular assignment of the relevant positions is referred to as an alignment. Another algorithm available in the state of the art is the FASTA algorithm. Sequence comparisons (alignments), especially multiple sequence comparisons, are created using computer programs. Frequently used, for example, are the Clustal series (see e.g. Chenna et al.(2003) Multiple sequence alignment with the Clustal series of programs, Nucleic Acid Res., 31:3497-3500), T-Coffee (cf. e.g. Notredame et al. (2000) T-Coffee: A novel method for multiple sequence alignments, J. Mol. Biol., 302:205-217) or programs based on these programs or algorithms. Furthermore, sequence comparisons (alignments) are possible with the computer program Vector NTI® Suite 10.3 (Invitrogen Corporation, 1600 Faraday Avenue, Carlsbad, California, USA) with the given standard parameters, whose AlignX module for sequence comparisons is based on ClustalW, or Clone Manager 10 (use of the BLOSUM 62 scoring matrix for sequence alignment at the amino acid level). Unless otherwise stated, sequence identity reported herein is determined using the BLAST algorithm.

[0709] Such a comparison also allows a statement to be made about the similarity of the compared sequences to one another. This is usually expressed as percent identity, i.e. the proportion of identical nucleotides or amino acid residues at the same positions or corresponding positions in an alignment. The broader term homology in amino acid sequences includes conserved amino acid substitutions, i.e. amino acids with similar chemical activity, since these usually exert similar chemical activities within the protein. Therefore, the similarity of the compared sequences can also be expressed as percent homology or percent similarity. Identity and / or homology statements can be made for entire polypeptides or genes, or just for individual regions. Homologous or identical regions of different nucleic acid or amino acid sequences are therefore defined by similarities in the sequences.Such regions often exhibit identical functions. They can be small and comprise only a few nucleotides or amino acids. Such small regions often perform essential functions for the overall activity of the protein. It may therefore be useful to refer sequence matches only to individual, possibly small regions. Unless otherwise stated, however, statements of identity or homology in this application refer to the entire length of the respective nucleic acid or amino acid sequence.

[0710] In the context of the present invention, the statement that an amino acid position corresponds to a numerically designated position in SEQ ID NO:1 therefore means that the corresponding position is assigned to the numerically designated position in SEQ ID NO:1 in an alignment as defined above. Furthermore, the assignment of the positions is based on the mature protein. This assignment is also to be applied in particular if the amino acid sequence of a protease according to the invention comprises a higher number of amino acid residues than the protease according to SEQ ID NO:1. Starting from the stated positions in the amino acid sequence of the protease according to SEQ ID NO:1, the change positions in a protease according to the invention are those that are assigned to precisely these positions in an alignment.

[0711] The following convention is used to describe substitutions that affect exactly one amino acid position (amino acid exchanges): first, the naturally occurring amino acid is named using the internationally accepted one-letter code, followed by the corresponding sequence position, and finally, the inserted amino acid. Multiple or alternative exchanges within the same polypeptide chain are separated by slashes. "130DA / " thus means that position 130 is mutated to D or V. In the case of insertions, additional amino acids are named after the sequence position. In the case of deletions, the missing amino acid is replaced by a symbol, such as an asterisk or a dash, or an A is indicated in front of the corresponding position.For example, P9T describes the substitution of proline at position 9 by threonine, P9TH the insertion of histidine after the amino acid threonine at position 9, and P9* or AP9 the deletion of proline at position 9. This nomenclature is known to those skilled in the art of enzyme technology.

[0712] Advantageous positions for sequence changes, in particular substitutions, of the protease according to SEQ ID NO:1, which, when transferred to homologous positions of the proteases according to the invention, are preferably of importance and impart advantageous functional properties to the protease, are accordingly the positions which, in an alignment, correspond to the positions described herein, ie, in the numbering according to SEQ ID NO:1. The following amino acid residues are located at the stated positions in the wild-type molecule of the protease: 3S, 4V, 74N, 99R, 136A, 143R, 154S, 160S, 161Y, 163A, 171V, 181A, 183F, 185Q, 199V, 200Q, 203Y, 209A, 212N and 256L.

[0713] In a further embodiment of the invention, a previously described protease is additionally stabilized, in particular by one or more mutations, for example substitutions, or by coupling to a polymer. Increasing stability during storage and / or use, for example during the washing process, results in longer-lasting enzymatic activity and thus improved cleaning performance. In principle, all stabilization options described and / or expedient in the prior art are contemplated. Preference is given to stabilizations achieved via mutations of the enzyme itself, since such stabilizations do not require any further steps following the isolation of the enzyme. Examples of suitable sequence modifications are mentioned above. Other suitable sequence modifications are known from the prior art.

[0714] Other stabilization options include:

[0715] Altering the binding of metal ions, in particular the calcium binding sites, for example by exchanging one or more of the amino acid(s) involved in calcium binding for one or more negatively charged amino acids and / or by introducing sequence changes in at least one of the sequences of the two amino acids arginine / glycine;

[0716] Protection against the influence of denaturing agents such as surfactants through mutations that cause a change in the amino acid sequence on or at the surface of the protein;

[0717] Exchange of amino acids located near the N-terminus for those that presumably come into contact with the rest of the molecule via non-covalent interactions and thus contribute to maintaining the globular structure.

[0718] Preferred embodiments are those in which the enzyme is stabilized in several ways, since several stabilizing mutations act additively or synergistically.

[0719] In addition, at least one further inhibitor compound selected from the group consisting of polyols, in particular glycerol and 1,2-propylene glycol, benzamidine hydrochloride, borax, boric acids, boronic acids or their salts or esters or derivatives, in particular phenylboronic acid derivatives or 4-formylphenylboronic acid (4-FPBA), and combinations thereof, may also be present.

[0720] For the purposes of the present invention, "phenylboronic acid derivative" refers to a compound of formula (III). The compound of formula (III) has the following structural formula: where R is hydrogen, a hydroxyl, a C1-6 alkyl, a substituted C1-6 alkyl, a C1-6 alkenyl, or a substituted C1-6 alkenyl group. Preferably, the R radical in the phenylboronic acid derivative is a C1-6 alkyl group, and among these, it is more preferably -CH3, -CH3CH2, or -CH3CH2CH2. More preferably, the R radical in the phenylboronic acid derivative is hydrogen. The phenylboronic acid derivative is particularly preferred as 4-formylphenylboronic acid (4-FPBA).

[0721] The additional inhibitor compound used can be boric acid.

[0722] Preferably, the agent according to the invention is substantially free of other inhibitor compounds. Most preferably, the agent according to the invention comprises, in addition to the compounds according to the invention having the formula (I) or (II), where R is each selected from -COOH, Ci-e-alkyl-substituted or unsubstituted C2-6-dicarboxylic acids, Ci-e-alkyl-substituted or unsubstituted C2-e-carboxylic acids and -OOC- NR 2 2, where R 2 are the same or different and selected from Ci-e-alkyl or H, as well as salts, esters or derivatives thereof, none of the other inhibitor compounds mentioned.

[0723] In particularly preferred embodiments, the washing and cleaning agent according to the invention is essentially free of boron-containing compounds. "Essentially free of boron-containing compounds" in this context means that the agents according to the invention contain less than 2% by weight, preferably less than 1% by weight, more preferably less than 0.5% by weight, and particularly preferably less than 0.1% by weight, of boron-containing compounds, based on the total weight of the agent. In very particularly preferred embodiments, the washing and cleaning agents according to the invention are free of boron-containing compounds, i.e., they contain in particular no boric acid and / or phenylboronic acid derivatives.

[0724] Using methods commonly known today, such as chemical synthesis or the polymerase chain reaction (PCR) in conjunction with standard molecular biological and / or protein chemical methods, it is possible for the skilled person to produce the corresponding nucleic acids, including complete genes, based on known DNA and / or amino acid sequences. Such methods are known, for example, from Sambrook, J., Fritsch, EF, and Maniatis, T. 2001. Molecular cloning: a laboratory manual, 3rd Edition, Cold Spring Laboratory Press.

[0725] In a further embodiment of the invention, the protease used according to the invention is characterized in that its cleaning performance is not significantly reduced compared to that of a protease comprising an amino acid sequence corresponding to the amino acid sequence given in SEQ ID NO:1, ie it has at least 80% of the reference washing performance, preferably at least 100%, more preferably at least 110% or more.

[0726] Washing or cleaning performance is understood to mean the ability of a washing or cleaning agent to partially or completely remove an existing soiling, in particular the lightening performance of one or more soils on textiles. Examples of such soils are blood on cotton or chocolate milk / soot on cotton, cocoa on cotton or porridge on cotton. Within the scope of the invention, both the washing or cleaning agent comprising the protease, or the washing or cleaning liquor formed by this agent, and the protease itself have a respective cleaning performance. The cleaning performance of the protease thus contributes to the cleaning performance of the agent or the washing or cleaning liquor formed by the agent.

[0727] The term "washing or cleaning liquor" refers to the working solution containing the washing or cleaning agent that acts on the textiles or hard surfaces and thus comes into contact with the soiling present on the textiles or hard surfaces. The washing or cleaning liquor is typically created when the washing or cleaning process begins and the washing or cleaning agent is diluted with water, for example, in a washing machine, dishwasher, or other suitable container.

[0728] The cleaning performance can be determined in a washing system that contains a detergent at a dosage of between 2.0 and 8.0 grams per liter of wash liquor and the enzyme. The enzymes to be compared are used at the same concentration (based on active protein). By using the same activity of the respective enzymes, it is ensured that even if there is a possible discrepancy in the ratio of active substance to total protein (the specific activity values), the respective enzymatic properties, for example the cleaning performance on specific soils, can be compared. In general, a low specific activity can be compensated for by adding a larger amount of protein. Furthermore, the enzymes to be tested can also be used in the same amount of substance or weight if the enzymes to be tested have a different affinity for the test substrate in an activity test.In this context, the term "equal amount of substance" refers to the same mole of the enzymes being tested. The term "equal amount by weight" refers to the same weight of the enzymes being tested.

[0729] The concentration of protease in the detergent intended for such a washing system is 0.001 to 0.1 wt.%, preferably 0.01 to 0.06 wt.%, based on active protein.

[0730] A liquid reference detergent for such a washing system can, for example, be composed as follows (all data in weight percent (wt%)): 4.4% alkylbenzenesulfonic acid, 5.6% other anionic surfactants, 2.4% C12-18 Na salts of fatty acids (soaps), 4.4% non-ionic surfactants, 0.2% phosphonates, 1.4% citric acid, 0.95% NaOH, 0.01% defoamer, 2% glycerin, 0.08% preservatives, 1% ethanol, and the remainder demineralized water. The preferred dosage of the liquid detergent is between 4.5 and 6.0 grams per liter of wash liquor, for example 4.7, 4.9, or 5.9 grams per liter of wash liquor. Washing is preferably carried out in a pH range between pH 7 and pH 10.5, preferably between pH 8 and pH 9.

[0731] The cleaning performance is determined by measuring the degree of cleanliness of the washed textiles compared to soiling on cotton. For example, the wash cycle can be carried out for 60 minutes at a temperature of 40°C and the water hardness can be between 15.5°dH and 16.5°dH (German hardness).

[0732] The degree of whiteness, i.e., the lightening of soils, as a measure of cleaning performance, is determined using optical measurement methods, preferably photometrically. A suitable device for this purpose is the Minolta CM508d spectrometer. The devices used for the measurement are usually calibrated beforehand with a white standard, preferably one supplied with the product.

[0733] Preferred embodiments of proteases according to the invention achieve such advantageous purification performance even at low temperatures, in particular in the temperature ranges between 10°C and 60°C, preferably between 15°C and 50°C, and particularly preferably between 20°C and 40°C. The protein concentration can be determined using known methods, for example the BCA method (bicinchoninic acid; 2,2'-biquinolyl-4,4'-dicarboxylic acid) or the biuret method (Gornall et al., 1948, J. Biol. Chem., 177:751-766). In this regard, the active protein concentration can be determined by titrating the active sites using a suitable irreversible inhibitor and determining the residual activity (Bender et al., 1966, J. Am. Chem. Soc. 88, 24:5890-5913).

[0734] According to the invention, a washing or cleaning agent is understood to mean all conceivable types of washing or cleaning agents, both concentrated and undiluted, for use on a commercial scale, in washing machines or for hand washing or cleaning. These include, for example, washing agents for textiles, carpets, or natural fibers, for which the term "washing agent" is used. This also includes, for example, dishwashing detergents for dishwashers (machine dishwashing detergents) or manual dishwashing detergents or cleaners for hard surfaces such as metal, glass, porcelain, ceramics, tiles, stone, painted surfaces, plastics, wood, or leather, for which the term "cleaning agent" is used. Thus, in addition to manual and machine dishwashing detergents, this also includes, for example, scouring agents, glass cleaners, toilet air fresheners, etc.The washing and cleaning agents within the scope of the invention also include washing aids that are added to the actual washing agent during manual or machine washing to achieve an additional effect. Furthermore, the washing and cleaning agents within the scope of the invention also include textile pre- and post-treatment agents, i.e. agents with which the laundry is brought into contact before the actual washing, for example, to dissolve stubborn soils, and also agents that impart further desirable properties to the laundry, such as a pleasant feel, resistance to creasing, or low static charge, in a step following the actual textile washing. Fabric softeners, among others, are counted among the latter agents.

[0735] The detergents or cleaning agents according to the invention, which may be in the form of powdered or granular solids, in compacted or recompacted particle form, as homogeneous solutions or suspensions, may contain, in addition to a protease according to the invention, all known ingredients customary in such agents, with at least one further ingredient preferably being present in the agent. The agents according to the invention may contain, in particular, surfactants, builders, polymers, glass corrosion inhibitors, corrosion inhibitors, bleaching agents such as peroxygen compounds, bleach activators, or bleach catalysts. They may also contain water-miscible organic solvents, other enzymes, enzyme stabilizers, sequestering agents, electrolytes, pH regulators, and / or other auxiliaries such as optical brighteners, graying inhibitors, dye transfer inhibitors, foam regulators, as well as dyes and fragrances, and combinations thereof.

[0736] Advantageous ingredients of agents according to the invention are disclosed in the international patent application WO 2009 / 121725, beginning on page 5, penultimate paragraph, and ending on page 13 after the second paragraph. This disclosure is expressly incorporated by reference, and the disclosure content therein is incorporated into the present patent application. An agent according to the invention advantageously contains the protease according to the invention in an amount of 2 pg to 20 mg, preferably 5 pg to 17.5 mg, particularly preferably 20 pg to 15 mg, and most preferably 50 pg to 10 mg per g of the agent. In various embodiments, the concentration of the protease (active enzyme) described herein in the agent is >0 to 1 wt.%, preferably 0.0001 or 0.001 to 0.1 wt.%, based on the total weight of the agent or composition.

[0737] An agent according to the invention contains the protease increasingly preferably in an amount of 1 x 10 -8 up to 5 wt.%, from 0.0001 to 1 wt.%, from 0.0005 to 0.5 wt.%, from 0.001 to 0.1 wt.%, each based on active protein and based on the total weight of the detergent.

[0738] The embodiments of the present invention encompass all solid, powdered, liquid, gel-like, or pasty dosage forms of agents according to the invention, which may optionally also consist of multiple phases and may be present in compressed or uncompressed form. The agent may be in the form of a free-flowing powder, in particular with a bulk density of 300 g / l to 1200 g / l, in particular 500 g / l to 900 g / l or 600 g / l to 850 g / l. Solid dosage forms of the agent also include extrudates, granules, tablets, or pouches. Alternatively, the agent may also be liquid, gel-like, or pasty, for example in the form of a non-aqueous liquid detergent or a non-aqueous paste, or in the form of an aqueous liquid detergent or a water-containing paste. Liquid agents are generally preferred. Furthermore, the agent may be in the form of a one-component system. Such agents consist of one phase.Alternatively, a remedy can also consist of multiple phases. Such a remedy is therefore divided into several components.

[0739] When the detergents according to the invention are in liquid form, they preferably contain more than 40% by weight, preferably 50 to 90% by weight and particularly preferably 60 to 80% by weight of water, based on their total weight.

[0740] The agents according to the invention may contain one or more surfactants, with particular preference being given to anionic surfactants, nonionic surfactants, and mixtures thereof, but cationic, zwitterionic, and / or amphoteric surfactants may also be present. The agents preferably contain 5 to 70% by weight of surfactant, more preferably 5 to 60% by weight, and more preferably 5 to 50% by weight of surfactant.

[0741] Suitable anionic surfactants are, in particular, soaps and those containing sulfate or sulfonate groups, preferably with alkali ions as cations. Suitable soaps are preferably the alkali salts of saturated or unsaturated C12-C18 fatty acids. Such fatty acids can also be used in a non-neutralized form. Suitable sulfate-type surfactants include the salts of the sulfuric acid monoesters of C12-C18 fatty alcohols and the sulfation products of the aforementioned nonionic surfactants with a low degree of ethoxylation. The usable surfactants of the sulfonate type include, for example, Cg-u-alkylbenzenesulfonates, alkanesulfonates obtained from Ci2-is-alkanes, for example by sulfochlorination or sulfoxidation with subsequent hydrolysis or neutralization, Ci2-18-olefinsulfonates, which are formed by the reaction of corresponding monoolefins with sulfur trioxide, mixtures of alkene and hydroxyalkanesulfonates, disulfonates, as can be found, for example, infrom Ci2-18 monoolefins with terminal or internal double bonds by sulfonation with gaseous sulfur trioxide and subsequent alkaline or acidic hydrolysis of the sulfonation products, as well as a-sulfofatty acid esters (ester sulfonates) which are formed during the sulfonation of fatty acid methyl or ethyl esters, e.g. a-sulfonated methyl esters of hydrogenated coconut, palm kernel or tallow fatty acids.

[0742] The agent preferably contains 2 to 55 wt.%, preferably 3 to 35 wt.%, of anionic surfactant. Most preferably, the agent contains 3 to 25 wt.% alkylbenzenesulfonate. Furthermore, the agent may preferably contain other anionic surfactants, especially alkyl ether sulfates, as well as nonionic surfactants, especially fatty alcohol alkoxylates. These can then make up the remainder of the surfactants.

[0743] Suitable alkylbenzenesulfonates are preferably selected from linear or branched alkylbenzenesulfonates of the formula in which R' and R" are independently H or alkyl and together contain 6 to 19, preferably 7 to 15 and in particular 9 to 13 C atoms. A particularly preferred representative is sodium dodecylbenzylsulfonate.

[0744] Preferred alk(en)yl sulfates are the alkali metal salts, especially the sodium salts, of the sulfuric acid half-esters of C12-18 fatty alcohols, e.g., coconut fatty alcohol, tallow fatty alcohol, lauryl, myristyl, cetyl, or stearyl alcohol, or C18-20 oxo alcohols, and those half-esters of secondary alcohols of these chain lengths. Also preferred are alk(en)yl sulfates of the stated chain length that contain a synthetic, petrochemically produced straight-chain alkyl radical, which exhibit degradation behavior similar to that of equivalent compounds based on oleochemical raw materials. For washing purposes, C12-18 alkyl sulfates and C12-15 alkyl sulfates, as well as C11-15 alkyl sulfates, are preferred.

[0745] Also suitable are the sulfuric acid monoesters of straight-chain or branched C1-2i alcohols ethoxylated with 1 to 6 mol of ethylene oxide, such as 2-methyl-branched C5-n alcohols with an average of 3.5 mol of ethylene oxide (EO) or C12-18 fatty alcohols with 1 to 4 EO.

[0746] Suitable alkyl ether sulfates are, for example, compounds of the formula

[0747] R 1 -O-(AO) n -SO3- X + .

[0748] In this formula, R 1 represents a linear or branched, substituted or unsubstituted alkyl radical, preferably a linear, unsubstituted alkyl radical, particularly preferably a fatty alcohol radical. Preferred radicals R 1 are selected from decyl, undecyl, dodecyl, tridecyl, tetradecyl, pentadecyl, hexadecyl, heptadecyl, octadecyl, nonadecyl, eicosyl radicals and mixtures thereof, with the representatives having an even number of carbon atoms being preferred. Particularly preferred radicals R 1are derived from C12-18 fatty alcohols, e.g., from coconut fatty alcohol, tallow fatty alcohol, lauryl, myristyl, cetyl, or stearyl alcohol, or from C12-20 oxo alcohols. AO stands for an ethylene oxide (EO) or propylene oxide (PO) group, preferably an ethylene oxide group. The index n stands for an integer from 1 to 50, preferably from 1 to 20, and in particular from 2 to 10. Most preferably, n stands for the numbers 2, 3, 4, 5, 6, 7, or 8. X + stands for a monovalent cation or the n-th part of an n-valent cation, preferably the alkali metal ions and among them Na + or K + , where Na + is highly preferred. Other cations X + can be selected from NF , % Zn 2+ , % Mg 2+ , % Ca 2+ , % Mn 2+ and their mixtures.

[0749] In various embodiments, the alkyl ether sulfate may be selected from fatty alcohol ether sulfates of the formula with k = 11 to 19, n = 2, 3, 4, 5, 6, 7, or 8. Particularly preferred representatives are Na-Ci2-Ci4 fatty alcohol ether sulfates with 2 EO (k = 11-13, n = 2). The stated degree of ethoxylation represents a statistical mean, which can be a whole or fractional number for a specific product. The stated degrees of alkoxylation represent statistical mean, which can be a whole or fractional number for a specific product. Preferred alkoxylates / ethoxylates have a narrow homolog distribution (narrow range ethoxylates, NRE).

[0750] It has proven beneficial for cold-wash performance if detergents also contain soap(s). Preferred detergents are therefore characterized by the presence of soap(s). Suitable soaps include saturated fatty acid soaps, such as the salts of lauric acid, myristic acid, palmitic acid, stearic acid, hydrogenated erucic acid, and behenic acid, as well as, in particular, soap mixtures derived from natural fatty acids, e.g., coconut, palm kernel, or tallow fatty acids.

[0751] Suitable nonionic surfactants are, in particular, alkyl glycosides and ethoxylation and / or propoxylation products of alkyl glycosides or linear or branched alcohols, each containing 8 to about 18 carbon atoms in the alkyl moiety and 3 to 20, preferably 4 to 10, alkyl ether groups. Furthermore, corresponding ethoxylation and / or propoxylation products of N-alkylamines, vicinal diols, fatty acid esters, and fatty acid amides, which correspond to the aforementioned long-chain alcohol derivatives with regard to the alkyl moiety, as well as of alkylphenols with 5 to 12 carbon atoms in the alkyl radical, are also suitable.

[0752] Nonionic surfactants used are preferably alkoxylated, advantageously ethoxylated, especially primary alcohols with preferably 8 to 18 carbon atoms and an average of 1 to 12 moles of ethylene oxide (EO) per mole of alcohol. The alcohol radical can be linear or, preferably, methyl-branched in the 2-position, or can contain linear and methyl-branched radicals in a mixture, as is usually the case in oxo alcohol radicals. However, alcohol ethoxylates with linear radicals from alcohols of native origin with 12 to 18 carbon atoms, e.g., from coconut, palm, tallow, or oleyl alcohol, and an average of 2 to 8 EO per mole of alcohol are particularly preferred. Preferred ethoxylated alcohols include, for example, C12-14 alcohols with 3 EO or 4 EO, C12-18 alcohols with 7 EO, C18-19 alcohols with 3 EO, 5 EO, 7 EO or 8 EO, C12-19 alcohols with 3 EO, 5 EO or 7 EO and mixtures thereof, such as mixtures of C12-14 alcohol with 3 EO and C12-18 alcohol with 5 EO.The stated degrees of ethoxylation represent statistical averages, which can be a whole or fractional number for a specific product. Preferred alcohol ethoxylates have a narrow homolog distribution (narrow range ethoxylates, NRE). In addition to these nonionic surfactants, fatty alcohols with more than 12 EO can also be used. Examples include tallow fatty alcohol with 14 EO, 25 EO, 30 EO, or 40 EO.

[0753] Another class of preferably used nonionic surfactants, which are used either as the sole nonionic surfactant or in combination with other nonionic surfactants, are alkoxylated, preferably ethoxylated or ethoxylated and propoxylated fatty acid alkyl esters, preferably having 1 to 4 carbon atoms in the alkyl chain, in particular fatty acid methyl esters.

[0754] Another class of nonionic surfactants that can be used advantageously are alkyl polyglycosides (APG). Suitable alkyl polyglycosides satisfy the general formula

[0755] RO(G)z, in which R is a linear or branched, in particular 2-methyl-branched, saturated or unsaturated, aliphatic radical having 8 to 22, preferably 12 to 18, carbon atoms, and G is the symbol representing a glycose unit having 5 or 6 carbon atoms, preferably glucose. The degree of glycosidation z is between 1 and 4, preferably between 1 and 2, and in particular between 1.1 and 1.4. Preference is given to using linear alkyl polyglycosides, i.e. alkyl polyglycosides in which the polyglycosyl radical is a glucose radical and the alkyl radical is an n-alkyl radical.

[0756] Nonionic surfactants of the amine oxide type, e.g., N-cocoalkyl-N,N-dimethylamine oxide and N-tallowalkyl-N,N-dihydroxyethylamine oxide, and fatty acid alkanolamides may also be suitable. The amount of these nonionic surfactants is preferably no more than that of the ethoxylated fatty alcohols, in particular no more than half that amount.

[0757] Suitable amphoteric surfactants are, for example, betaines of the formula

[0758] (R iii )(R iv )(R v )N + CH2COO-, in which R'" is an alkyl radical having 8 to 25, preferably 10 to 21 carbon atoms, optionally interrupted by heteroatoms or heteroatom groups, and R iv and R v identical or different alkyl radicals having 1 to 3 carbon atoms, in particular C10-18-alkyldimethylcarboxymethylbetaine and Cn-17-alkylamidopropyldimethylcarboxymethylbetaine.

[0759] Suitable cationic surfactants include the quaternary ammonium compounds of the formula (Rvi )(R vii )(R viii )(R ix )N + X-, in the R vi to R ixstands for four identical or different, in particular two long-chain and two short-chain, alkyl radicals and X- stands for an anion, in particular a halide ion, e.g. didecyldimethylammonium chloride, alkylbenzyldidecylammonium chloride and mixtures thereof. Other suitable cationic surfactants are quaternary surface-active compounds, in particular with a sulfonium, phosphonium, iodonium or ar...

Claims

PATENT CLAIMS 1. Washing and cleaning agents, in particular liquid washing and cleaning agents, especially preferably liquid textile detergent, comprising (A) at least one protease, wherein the protease has proteolytic activity and comprises an amino acid sequence having at least 70% sequence identity with the amino acid sequence specified in SEQ ID NO:1 over its total length and, with respect to the numbering according to SEQ ID NO:1, (i) at least two, preferably three, of the positions corresponding to positions 3, 4, 99 or 199, at least two, preferably three, amino acid substitutions selected from the amino acid substitutions 3T, 4I, 99E and 199I, and (ii) at least one further substitution at at least one of the positions corresponding to positions 74, 136, 143, 154, 160, 161, 163, 171, 181, 183, 185, 200, 203, 209, 212 or 256 Amino acid substitution, in particular selected from N74D / E / Q, A136Q, R143L / W / Y, S154D / Q, S160G, Y161T, A163G, V171 L, A181 D, F183R, Q185R, Q200A / L / S / T, Y203K / V / W, A209K / W, N212S / T and L256D / E / Q, preferably in an amount of 0.0001 to 1 wt.-%, based on the total weight of the washing and cleaning agent. (B) at least one compound with formula (I) or (II) where R is selected from -COOH, Ci-e-alkyl-substituted or unsubstituted C2-6-dicarboxylic acids, Ci-e-alkyl-substituted or unsubstituted C2-e-carboxylic acids and -OOC- NR 2 2, where R 2 the same or different and selected from Ci-e-alkyl or H, as well as salts, esters or derivatives thereof, preferably in an amount of 0.1 to 2 wt.%, based on the total weight of the washing and cleaning agent, (C) optionally at least one further enzyme, wherein the further enzyme is different from the at least one protease, preferably in an amount of 0.0001 to 1% by weight, based on the total weight of the washing and cleaning agent, (D) at least one ingredient, preferably in an amount of 0.01 to 99.9% by weight, based on the total weight of the washing and cleaning agent.

2. Washing and cleaning agent according to claim 1, wherein the protease comprises an amino acid sequence that is at least 70% and increasingly preferably at least 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 90.5%, 91%, 91.5%, 92%, 92.5%, 93%, 93.5%, 94%, 94.5%, 95%, 95.5%, 96%, 96.5%, 97%, 97.5%, 98%, 98.5% and 98.8% identical, and, in each case with respect to the numbering according to SEQ ID NO:1, (i) at the positions corresponding to positions 3, 4, 99 or 199, the amino acid substitutions S3T, V4I, R99E and V199I, and (ii) at at least one of the positions corresponding to positions 154, 160, 181, 183, 185, 200, 203, 209 or 256, at least one and increasingly preferably two, three, four, five or six amino acid substitution(s) consisting of the The existing group includes S154D, S160G, A181 D, F183R, Q185R, Q200L, Y203W, A209K and L256E.

3. Washing and cleaning agent according to any of the preceding claims, wherein the protease has one of the following amino acid substitution variants, each with reference to the numbering according to SEQ ID NO:1: (i) S3T-V4I-R99E-V199I-Q200L-Y203W; (ii) S3T-V4I-R99E-V199I-N212S; (iii) S3T-V4I-R99E-V199I-N74D; (iv) S3T-V4I-R99E-V199I-S154D-L256E; (v) S3T-V4I-R99E-V199I-Q200L-Y203W-S154D-L256E; (vi) S3T-V4I-R99E-V199I-N74D-Q200L-Y203W; (vii) S3T-V4I-R99E-V199I-N74D-S154D-Q200L-Y203W-L256E; (viii) S3T-V4I-R99E-V199I-N74D-N212S; (ix) S3T-V4I-R99E-V199I-N74D-S154D-Y203W-L256E; (x) S3T-V4I-R99E-V199I-N74D-Y203W; (xi) S3T-V4I-R99E-V199I-N74D-S154D-Q200L-L256E; (xii) S3T-V4I-R99E-V199I-N74D-Q200L; (xiii) S3T-V4I-R99E-V199I-S154D-Q200L-Y203W; (xiv) S3T-V4I-R99E-V199I-Q200L-Y203W-L256E; (xv) S3T-V4I-R99E-V199I-A136Q-R143W-Y161 T-Q200L; (xvi) S3T-V4I-R99E-V199I-N74D-R143Y-A209W-N212S-L256E; (xvii)S3T-V4l-R99E-V199I-A136Q-S154D-V171 L-Q200L; (xviii) S3T-V4I-R99E-V199I-Q200L-Y203W-A209K-S154D-L256E; (xix) S3T-V4I-R99E-V199I-S154D-S160G-Q185R-Q200L-Y203W-L256E; (xx) S3T-V4I-R99E-V199I-S154D-A181 D-F183R-Q200L-Y203W-L256E.

4. Washing and cleaning agent according to any of the preceding claims, wherein compound (B) is selected from the group consisting of benzoic acid, phenylmalonic acid, benzylmalonic acid, phenylsuccinic acid, benzylsuccinic acid, methyl 3-benzoylpropionate, (S)-3-phenylbutyric acid and benzylcarbamate.

5. Washing and cleaning agent according to one of the preceding claims, wherein the at least one further enzyme is selected from the group consisting of amylases, cellulases, hemicellulases, mannanases, tannases, xylanases, xanthanases, xyloglucanases, β-glucosidases, pectinases, carrageenases, perhydrolases, oxidases, oxidoreductases, lipases, proteases and combinations thereof, preferably at least one amylase and / or at least one cellulase and / or at least one lipase and / or at least one mannanase.

6. Washing and cleaning agent according to any of the preceding claims, wherein the at least one further enzyme is an amylase and wherein the amylase is selected from (a) an α-amylase comprising an amino acid sequence that is at least 80% identical over its total length to the amino acid sequence specified in SEQ ID NO:2 and optionally includes at least one amino acid substitution at one of positions 172, 202, 208, 255 and 261 in the numbering according to SEQ ID NO:2, preferably selected from the group consisting of M202L, M202V, M202S, M202T, M202I, M202Q, M202W, S255N, R172Q and combinations thereof; and / or (b) an α-amylase comprising an amino acid sequence that is identical to the amino acid sequence specified in SEQ ID NO:3 over its total length to at least 60% and optionally includes at least one amino acid substitution at one of positions 9, 26, 30, 33, 82, 37, 106, 118, 128, 133, 149, 150, 160, 178, 182, 186, 193, 195, 202, 203, 214, 231, 256, 257, 258, 269, 270, 272, 283, 295, 296, 298, 299, 303, 304, 305, 311, 314, 315, 318, 319, 320, 323, 339, 345, 361, 378, 383, 419, 421, 437, 441, 444, 445, 446, 447, 450, 458, 461, 471, 482 and 484 and / or a deletion at one of positions 183 and 184 in the numbering according to SEQ ID NO:3, preferably at least one amino acid substitution at one of positions 9, 26, 149, 182, 186, 202, 257, 295, 299, 323, 339 and 345, and / or particularly preferably at least one amino acid substitution or deletion selected from the group consisting of R118K, D183*, G184*, N195F, R320K, R458K and combinations thereof; and / or (c) an α-amylase comprising an amino acid sequence that is identical to the amino acid sequence specified in SEQ ID NO:4 over its total length to at least 65% and optionally includes at least one substitution and / or deletion at one of positions 93, 116, 118, 129, 133, 134, 140, 142, 146, 147, 149, 151, 152, 169, 174, 183, 184, 186, 189, 193, 195, 197, 198, 200, 203, 206, 210, 212, 213, 235, 243, 244, 260, 262, 284, 303, 304, 320, 338, 347, 359, 418, 431, 434, 439, 447, 458, 469, 476 and 477 in the numbering according to SEQ ID NO:4, preferably amino acid deletions at positions 183 and 184; and / or (d) an α-amylase comprising an amino acid sequence that is identical to the amino acid sequence specified in SEQ ID NO:5 over its total length to at least 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% and 100% and has at least one deletion at one of positions 180, 181, 182, 183 and 184 in the count according to SEQ ID NO:5, preferably deletions at at least two positions selected from positions 180+181, 181+182, 182+183 and 183+184 in the numbering according to SEQ ID NO:5, particularly preferably at positions 183+184 in the numbering according to SEQ ID NO:5, and / or has at least one substitution at one of positions 405, 421, 422 and 428 in the numbering according to SEQ ID NO:5, which is selected from the group consisting of I405L, A421 H, A422P, A428T and combinations thereof.

7. Washing and cleaning agent according to any of the preceding claims, wherein the at least one further enzyme is a cellulase.

8. Washing and cleaning agent according to any of the preceding claims, wherein a) the at least one protease is a protease according to any of claims 1 to 3, b) the at least one further enzyme is an amylase according to claim 6 and / or a cellulase.

9. Washing and cleaning agents according to any of the preceding claims, wherein the detergent ingredient is selected from the group consisting of surfactants, builders, complexing agents, polymers, glass corrosion inhibitors, corrosion inhibitors, bleaching agents such as peroxygen compounds, bleach activators or bleach catalysts, water-miscible organic solvents, enzyme stabilizers, sequestrants, electrolytes, pH regulators and / or further auxiliary substances such as optical brighteners, graying inhibitors, color transfer inhibitors, foam regulators, as well as dyes and perfumes and combinations thereof.

10. Washing and cleaning agent according to any of the preceding claims, wherein the washing and cleaning agent is substantially free of boron-containing compounds, preferably free of boron-containing compounds.

11. Washing and cleaning agent according to one of the preceding claims, wherein in a 1 wt.% solution in deionized water at 20°C it has a pH value in a range of about 6 to about 11, in particular from about 6.5 to about 10.5, more preferably from about 7 to about 10, particularly preferably from about 8 to about 9.

12. Method for cleaning textiles and / or hard surfaces, characterized in that in at least one process step a washing or cleaning agent according to one of claims 1 to 11 is used, wherein the method is preferably carried out in a temperature range of about 20°C to about 60°C, particularly preferably from about 20°C to about 40°C, most preferably from about 30°C.

13. Use of a washing or cleaning agent according to any one of claims 1 to 11 for cleaning textiles and / or hard surfaces, preferably in a temperature range of about 20°C to about 60°C, particularly preferably from about 20°C to about 40°C, and most preferably from about 30°C.

14. Use of a compound with formula (I) or (II) where R is selected from -COOH, Ci-e-alkyl-substituted or unsubstituted C2-6-dicarboxylic acids, Ci-e-alkyl-substituted or unsubstituted C2-6-carboxylic acids and -OOC- NR 2 2, where R 2 the same or different and selected from Ci-e-alkyl or H, as well as salts, esters or derivatives thereof, to improve the stability, in particular the storage stability, of enzymes, preferably proteases, cellulases and / or amylases, in a washing and cleaning agent, preferably in a washing or cleaning agent according to any one of claims 1 to 11.

15. Use of a compound with formula (I) or (II) where R is selected from -COOH, Ci-e-alkyl-substituted or unsubstituted C2-6-dicarboxylic acids, Ci-e-alkyl-substituted or unsubstituted C2-e-carboxylic acids and -OOC- NR 2 2, where R 2 the same or different and selected from Ci-e-alkyl or H, as well as salts, esters or derivatives thereof, for preventing and / or reducing the proteolysis of further enzymes contained in the washing and cleaning agent, preferably amylases and / or cellulases, preferably cellulases, by a protease contained in the washing and cleaning agent, wherein the washing and cleaning agent is preferably a washing and cleaning agent according to any one of claims 1 to 11.

16. Use of a compound with formula (I) or (II) (II) where R is selected from -COOH, Ci-e-alkyl-substituted or unsubstituted C2-6-dicarboxylic acids, Ci-e-alkyl-substituted or unsubstituted C2-e-carboxylic acids and -OOC-NR 2 2, where R 2 the same or different and selected from Ci-e-alkyl or H, as well as salts, esters or derivatives thereof, for preventing and / or reducing the autoproteolysis of a protease contained in the washing and cleaning agent, wherein the washing and cleaning agent is preferably a washing and cleaning agent according to any one of claims 1 to 11.

17. Use according to any one of the preceding claims 14 to 16, wherein the compound is selected from the group consisting of benzoic acid, phenylmalonic acid, benzylmalonic acid, phenylsuccinic acid, benzylsuccinic acid, methyl 3-benzoylpropionate, (S)-3-phenylbutyric acid and benzylcarbamate.

18. Use according to any one of the preceding claims 14 to 17, wherein a) the one protease is a protease according to any one of claims 1 to 3, b) the at least one further enzyme is an amylase according to claim 6 and / or a cellulase.

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