Pressure supply unit and method for providing a first target pressure level and a second target pressure level for a microfluidic analysis system, microfluidic analysis system

Abstract

A pressure supply unit for providing a first target pressure level (1051) and a second target pressure level (1051), comprising - a pump (103) for drawing in a fluid, - a first component (1011, 1071) for controlling fluid flow on a first side (1001) of the pump (103) that controls the fluid flow according to a first current pressure level (1053) and is thus configured to provide the first target pressure level (1051) on the first side (1001) of the pressure supply unit (100), and - a second component (1012, 1072) for controlling fluid flow on a second side (1002) of the pump (103) that controls the fluid flow according to a second current pressure level (1054) and is thus configured to provide the second target pressure level (1052) on the second side (1002) of the pressure supply unit (100). The invention further relates to a method for providing two target pressure levels (1051, 1052) and to a microfluidic analysis system.

Description

[0001] Description

[0002] title

[0003] Pressure supply unit and method for providing a first target pressure level and a second target pressure level for a microfluidic analysis system, microfluidic analysis system

[0004] State of the art

[0005] DE 102018 114 150 A1 discloses a device and a method for separating a fluidic sample, wherein the sample separation device has only one pump for moving the respective mobile phase for all separation stages. Given this implementation of only a single high-pressure pump as the fluid drive, the first-dimensional analysis and the second-dimensional analysis are not performed in parallel, but sequentially.

[0006] Core and advantages of the invention

[0007] The invention relates to a pressure supply unit for providing a first target pressure level and a second target pressure level, a method for providing a first target pressure level and a second target pressure level, a microfluidic analysis system, furthermore a control device which uses the aforementioned method, and finally a corresponding computer program according to the main claims.

[0008] An advantage of the invention with the features of the independent patent claims is that a compact pressure supply unit with a simplified structure and simplified control for providing two different target pressure levels, i.e., specific or fixed pressure levels, is provided, which can also be manufactured cost-effectively. This is achieved with a pressure supply unit according to claim 1 for providing a first target pressure level and a second target pressure level, wherein the first target pressure level and the second target pressure level differ from one another. The pressure supply unit comprises a pump for sucking in a fluid, in particular a gas, such as air, a first component for fluid flow control, which

[0009] ■ is arranged on a first side of the pump, and

[0010] ■ to enable fluid flow

[0011] • from the first side of the pressure supply unit to the pump, provided that a first current pressure level on the first side is greater than the first target pressure level, and

[0012] • from an environment of the pressure supply unit to the pump, provided that the first current pressure level on the first side is less than the first target pressure level or equal to the first target pressure level, and is thus configured to provide the first target pressure level on the first side of the pressure supply unit, and a second component for fluid flow control, which

[0013] ■ is arranged on a second side of the pump, and

[0014] ■ to enable fluid flow

[0015] • from the pump to the second side of the pressure supply unit, provided that a second current pressure level on the second side is lower than the second target pressure level, and

[0016] • from the pump into the environment of the pressure supply unit, provided that a second current pressure level on the second side is greater than the second target pressure level or equal to the second target pressure level, and is thus set up to provide the second target pressure level on the second side of the pressure supply unit.

[0017] Pressure supply units are designed to generate pressure levels and to supply other systems that are or can be connected to the pressure supply unit with fluid, in particular compressed air. The pressure supply unit according to claim 1 is designed to supply other systems with a fluid having a specific pressure (for example, compressed air), i.e., for example, with a high-pressure level, in particular up to 3000 mbarA, and / or a vacuum pressure level, in particular up to 200 mbarA. The pressure supply unit can be connected to other systems, for example, via at least one pneumatic interface.

[0018] The term “target pressure level” is understood to mean, in particular, a target pressure value, such as 400 mbarA (millibar) or 2600 mbarA, with a tolerance range around this target pressure value, such as + / - 100 mbar, where: 1 bar = 10 5Pa. The target pressure levels are the pressure levels that can be provided by the pressure supply unit for a consumer / system. For example, the first target pressure level corresponds to 400 mbarA + / - 100 mbar, and the second target pressure level corresponds to 2500 mbarA + / - 100 mbar.

[0019] The term "current pressure level" refers to a current pressure value, such as 2300 mbarA. The current pressure levels are adjusted by the pump and the fluid flow control components until they correspond to the respective target pressure levels, i.e., in particular, they are within the tolerance range around the target pressure values.

[0020] The pressure supply unit is arranged in an environment in which a fluid, in particular a liquid or a gas, such as air, can be arranged. The environment is characterized by being filled with a fluid or being fillable with such a fluid. The environment can in particular comprise the entirety of what surrounds the pressure supply unit; it can be designed as an open volume or a limited volume, such as a room, a housing, a container, a fluid reservoir, etc.

[0021] The pump for sucking in the fluid, in particular a gas and / or a liquid, can for example be designed as a single pump, for example as a double-head pump. In particular, the pump can comprise a diaphragm pump, a blower or a compressor. The pump is designed to convey the fluid. The pump connects reservoirs, i.e. containers or areas for holding the fluid, to one another and can transport the fluid between the reservoirs, i.e. in particular suck it in from one reservoir and pass it on to another reservoir. For example, the pump can suck in the fluid from an environment of the pressure supply unit and pass it on to a system in which the fluid is required, such as a pressure vessel, a microfluidic analysis system for carrying out a sample analysis, in particular the test module units of the analysis system, etc.

[0022] The flow is transmitted in particular by components for fluid flow control, especially valves such as check valves or switching valves such as 3 / 2-way valves. Fluid flow control is understood in particular to include blocking / stopping a fluid flow, mixing fluid streams, and redirecting or redirecting the fluid flow.

[0023] The pump is arranged between two sides of the pressure supply unit, the first side and the second side. The sides, in particular, designate areas in which different pressure levels can be set. For example, containers, such as hydraulic accumulators or pressure vessels, can be arranged on the different sides, in each of which a pressure corresponding to the respective target pressure level can be generated. Furthermore, components for fluid flow control as well as lines that connect the various units and components to one another and through which the fluid can move between the various units and components are arranged on the sides.Each side can be connected, for example, by means of an interface or a connection for fluid transmission to a system or subsystem, in particular a test module of a microfluidic analysis unit, and can provide a pressure level for this by forwarding the fluid of the respective side, ie, for example, supply the system with compressed air at a target pressure level set by the pressure supply unit.

[0024] One advantage is that a compact pressure supply unit can be realized by designing the supply circuit so that both target pressure levels can be generated with a single pump and made available to a system connected to the pressure supply unit. Furthermore, this advantageously eliminates the need for a pump in the overall pressure generation system, which not only reduces installation space, but also, above all, reduces costs and the complexity of the control system with regard to parallel control methods. Another positive effect is that the number of potential noise sources (pumps) is reduced, making the system quieter and therefore more user-friendly.

[0025] In one embodiment, the first component for controlling the fluid flow can be connected to a first storage volume, in particular a first fluid reservoir, in particular a gas reservoir, and / or the second component for controlling the fluid flow can be connected to a second storage volume, in particular a second fluid reservoir, in particular a gas reservoir. One advantage is that pressure can thus be stored in the container / volumes on the respective side of the pressure supply unit and made available to the system.

[0026] Another advantage is that this increases the elasticity of the system and cushions pressure peaks that can arise from possible switching of the flow control components (especially the valves), from the start-up of the pump or from design-related pulsations. In this case, it is recommended that a volume of 5-10 percent of the volume flow at the design operating point be selected if only a pure spring effect via the volumes is desired. If the storage function is also to be addressed with the pressure supply unit, which is particularly important during shutdown operation, the volume must be dimensioned accordingly depending on the components installed in the system and should be designed so that the system components can be supplied from the storage for their function for 30 to 120 seconds when the pump is not in operation.

[0027] In one embodiment, the first side is designed as a low-pressure region and the second side as a high-pressure region, wherein a low-pressure level can be provided as the first target pressure level in the low-pressure region, and wherein a high-pressure level can be provided as the second target pressure level in the high-pressure region. A system connected to this pressure supply unit can thus advantageously be supplied with a low-pressure level and a high-pressure level, wherein only one pump is used for this purpose instead of two. In one embodiment, the first target pressure level and the second target pressure level each have a target pressure value of 400 mbarA, 2500 mbarA or a target pressure value in the range of 400 mbarA and 2500 mbarA system pressure, as well as a tolerance range around the respective target pressure value, in particular + / - 100 mbar, wherein the first target pressure level and the second target pressure level differ from one another, iein particular that the respective target pressure values ​​of the target pressure levels differ from each other.

[0028] In one embodiment, at least one pressure sensor unit for pressure regulation and / or for controlling a safety shutdown is arranged on the first side and / or the second side. In particular, this allows the current pressure levels to be measured. Furthermore, monitoring and intermittent operation of the pump are thus possible in order to reduce its running time and thus, in particular, minimize noise generation. The pressure sensor unit can be used to implement safety shutdowns, as well as simple pressure regulation and shutdown of the pressure supply unit. The pressure sensor unit can comprise, for example, a MEMS pressure sensor.

[0029] In one embodiment, the first component for fluid flow control and the second component for fluid flow control are dimensioned such that a pressure loss across these components is less than or equal to 5% of the system pressure (the system pressure here corresponds to the ambient pressure, i.e. the pressure of the environment), as the hysteresis would otherwise be too dependent on the system. In general, the pressure loss should be very small. In pneumatics, pressure losses tend to be very small compared to hydraulics. The aim is to keep the pressure loss across the valves small compared to the working pressure so as not to limit the efficiency of the pump too much. This should not exceed 5 percent of the system pressure and should be chosen to be as minimal as possible. One advantage is that this can increase the efficiency and reliability of the pressure supply unit.

[0030] According to one embodiment, the first component and / or the second component for controlling the fluid flow comprise a switching valve, in particular a 3 / 2-way valve. One advantage is that the target pressure levels on the sides with the switching valve can be variably adjusted, thus allowing the pressure supply unit to be used flexibly and adapted to the needs of the connected or connectable system / consumer.

[0031] In one embodiment, the first component and / or the second component for controlling the fluid flow comprise a check valve. The target pressure level in the respective circuit (i.e., on the respective side) is set via the opening pressure of the spring of the respective check valve. Instead of switching valves, passive check valves are used for both components, for example, whose springs are dimensioned such that they: on the first side (here: low-pressure orVacuum side) open when the vacuum pressure (first current pressure level) falls below a critical pressure value, for example 400 mbarA (opening here means that the fluid on the first side can flow from the environment into the pressure supply unit); on the second side (here: high pressure side) open when the high pressure (second current pressure level) is above a critical pressure value, for example 2500 mbarA (opening here means that the fluid on the second side can escape into the environment).

[0032] One advantage is that this allows for a very simple pressure supply unit to be implemented, enabling further cost and complexity reductions. This type of design can be selected in particular when the first and second target pressure levels are fixed and should not be variably adjustable, corresponding to a fixed operating point. Check valves advantageously allow the pressure range to be fixed and very precisely defined using hardware, thus providing a robust solution.

[0033] Advantages of a method for providing a first target pressure level and a second target pressure level, in particular using one of the above-mentioned pressure supply units, wherein the first target pressure level and the second target pressure level differ from one another, comprising the following method steps:

[0034] Providing the first target pressure level on the first page of the

[0035] Pressure supply unit by enabling a fluid flow ■ from the first side of the pressure supply unit to the pump, provided that a first current pressure level on the first side is greater than the first target pressure level, and

[0036] ■ from an environment of the pressure supply unit to the pump, provided that the first current pressure level on the first side is less than the first target pressure level or equal to the first target pressure level,

[0037] Providing the second target pressure level on the second side of the pressure supply unit by forwarding the fluid

[0038] ■ from the pump to the second side of the pressure supply unit, provided that a second current pressure level on the second side is lower than the second target pressure level, and

[0039] ■ from the pump into the environment of the pressure supply unit, provided that a second current pressure level on the second side is greater than the second target pressure level or equal to the second target pressure level, arise directly from the advantages mentioned for the pressure supply unit. In particular, it is advantageous that two different target pressure levels can be provided for a system / consumer by means of a pump, in particular a single pump, by controlling the fluid flow depending on the current pressure levels, i.e. in other words, the fluid flows / flows depending on the current pressure levels either between the two sides of the pressure supply unit or between the environment and one side in each case and the target pressure levels on the two sides can therefore be set without using an additional pump.The diversion / redirection of the fluid flow depending on the respective current pressure level is achieved in particular by means of the components for fluid flow control, which are connected to the pump, the environment, and interfaces for connecting a consumer (e.g., a system or subsystem, a microfluidic analysis system, or a test module unit of a microfluidic analysis system, etc.) via fluid lines (also called fluid channels) through which the fluid can flow. Another advantage is that when the pressures upstream and downstream of the pump are within the target pressure range, the pump does not need to be operated and can, for example, operate only on demand. "Demand-controlled" here means when at least one of the target pressures is not within the limit.

[0040] This method can be implemented, for example, in software or hardware or in a mixed form of software and hardware, for example in a control unit.

[0041] In one embodiment, when the first target pressure level is reached on the first side and the second target pressure level is reached on the second side, the pump of the pressure supply unit is switched off (= switch-off mode of the pressure supply unit) or the pump continues to operate (= continuous operation of the pressure supply unit), whereby during continued operation the fluid is sucked in from the environment and passed to the environment by the pump. Continuous operation is particularly useful when the proportion of this operating phase is very short in time compared to the other three operating modes (= generating high pressure, generating low pressure, generating high and low pressure), for example when the time proportion is less than 10 percent (t < 10 percent). Otherwise, it may be more efficient to operate the pump in switch-off mode.

[0042] The microfluidic analysis system may comprise a pressure supply unit according to one of the aforementioned embodiments or be connectable to such a unit, in particular by connecting the fluid channels of the pressure supply unit via interfaces, in particular pneumatic interfaces, to fluid channels of the test module unit. Through this connection, the test module unit is supplied with the first target pressure level and the second target pressure level, which can be provided by the pressure supply unit at the connections / (pneumatic) interfaces of the pressure supply unit.

[0043] Advantages of a microfluidic analysis system for carrying out a sample analysis, comprising a test module unit for sample intake for a molecular diagnostic analysis, comprising

[0044] • a pressure supply unit according to one of the embodiments described above for providing the first target pressure level and the second target pressure level, • peripheral components for carrying out a molecular diagnostic analysis and

[0045] • microfluidic elements for sample transport in the test module unit,

[0046] - wherein the microfluidic elements of the test module unit can be controlled by means of the first target pressure level and / or the second target pressure level, and

[0047] - wherein the pressure supply unit is connected or connectable to the test module unit via an interface. These advantages arise from the pressure supply unit described above.

[0048] A microfluidic analysis system is a small pneumatic device, especially a medical device.

[0049] An example of a microfluidic analysis system is the VivalyticO platform (Robert Bosch GmbH), which is a universal diagnostic platform that can perform various single or multiplex tests in one cartridge.

[0050] In microfluidic analysis systems for sample analysis, the sample material, such as a swab, blood, urine, etc., is first treated with ultrasound. This opens the cell membrane and releases the DNA and RNA molecules. In the next step, they are filtered, amplified, and detected. Even the smallest amounts of DNA and RNA in the sample material can be detected.

[0051] The microfluidic analysis system comprises at least one test module unit, which includes all the necessary peripheral components to perform a molecular diagnostic analysis. Such peripheral components can, for example, include, among others, the following components: heating elements, a cartridge insertion mechanism, a pneumatic manifold for distributing and controlling valves, a clamping device for the cartridge, and pressure vessels (i.e., pneumatic accumulators) to hold a minimum amount of compressed air as a reservoir, and / or an optical unit to detect the fluidic reaction results within the cartridge. The optical unit can be used for sample analysis, such as for fluorescence measurements on the sample, and can, in particular, comprise an optical sensor unit and an illumination source.

[0052] The test module is supplied with pressure (i.e., the first target pressure level and the second target pressure level) and typically does not include any internal means of pressure generation, but rather only connections / interfaces through which the pressurized fluid, such as compressed air, can be supplied from the pressure supply unit. Alternatively, the pressure supply unit can be integrated into the test module, meaning, in particular, that they are then permanently connected to one another.

[0053] The term "sample transport" can be understood in particular as the movement of the sample, in particular a sample liquid, within the test module unit. In other words, sample transport is in particular a fluid transport.

[0054] The sample is collected by the test module unit by inserting a cartridge into the test module unit, with the sample first being inserted into the cartridge. The test module unit then processes the sample partially or fully automatically within the cartridge, for example, for sample analysis, particularly for conducting diagnostic tests. The sample is, in particular, a sample liquid.

[0055] The microfluidic elements can, in particular, be microfluidic valves and (pumping) chambers. At least two pressure levels, in this case the target pressure levels, are used to control the microfluidic elements. In particular, the control and provision of the target pressure levels are carried out by the pressure supply unit, which has a pneumatic interface to the test module unit.

[0056] The test module unit can, for example, comprise one or more test modules, which are supplied with the first and second target pressure levels by the pressure supply unit. For this purpose, the test module unit can be connected or connectable to a fluid line on the first and second sides of the pressure supply unit, in particular via a connection or a pneumatic interface. The use of the pressure supply unit described above enables pressure supply for two different target pressure levels with only one pump, in particular a single pump.

[0057] In one embodiment, parallel control of multiple test module units by a common pressure supply unit according to one of the embodiments described above is possible, thus enabling, for example, a rack solution for the microfluidic analysis system. This can then be supplied as a whole or in several discrete units by corresponding pressure supplies.

[0058] For example, the first target pressure level is vacuum up to 200 mbarA, and the second target pressure level is pressures up to 3000 mbarA. In particular, the working range is at target pressure levels of 400 mbarA and 2600 mbarA.

[0059] An example of a microfluidic analysis system that is supplied entirely by the target pressure levels (i.e., both test module units are each provided with both target pressure levels) is a microfluidic analysis system that diagnoses cartridges by controlling valves on each cartridge via the two target pressure levels, thus allowing fluids on the cartridge to be moved, directed, controlled, and / or mixed. Using the pressure supply unit described above advantageously makes it possible to set both working pressures and provide them to the cartridge with a single pump.

[0060] The approach presented here further creates a control unit that is designed to carry out, control, or implement the steps of a variant of a method presented here in corresponding devices or units. This embodiment of the invention in the form of a control unit also allows the problem underlying the invention to be solved quickly and efficiently. For this purpose, the control unit can have at least one computing unit for processing signals or data, at least one memory unit for storing signals or data, at least one interface to a sensor or an actuator for reading in sensor signals from the sensor or for outputting control signals to the actuator, and / or at least one communication interface for reading in or outputting data.In this case, a control unit can be understood as an electrical device that processes sensor signals and outputs control and / or data signals depending on them.

[0061] Also advantageous is a computer program product or computer program with program code that can be stored on a machine-readable carrier or storage medium such as a semiconductor memory, a hard disk memory or an optical memory and is used to carry out, implement and / or control the steps of the method of one of the embodiments described above, in particular when the program product or program is executed on a computer or a device.

[0062] Short description of the drawings

[0063] Embodiments of the invention are illustrated in the drawings and explained in more detail in the following description. Identical reference numerals in the figures denote identical or equivalent elements.

[0064] It shows

[0065] Fig. 1 shows a schematic structure of a pressure supply unit according to a first embodiment,

[0066] Fig. 2 shows a schematic structure of a pressure supply unit according to a second embodiment,

[0067] Fig. 3 is a flowchart of a method for providing a first target pressure level and a second target pressure level,

[0068] Fig. 4 is a schematic sketch of a microfluidic analysis system according to a third embodiment,

[0069] Fig. 5 is a schematic sketch of a microfluidic analysis system according to a fourth embodiment, and

[0070] Fig. 6 is a schematic sketch of a microfluidic analysis system according to a fifth embodiment.

[0071] Embodiments of the invention. Fig. 1 shows a schematic structure of a pressure supply unit 100 according to an embodiment, in particular a basic connection option for a single-circuit system solution. The pressure supply unit 100 comprises a pump 103, which is arranged between a first side 1001 and a second side 1002 of the pressure supply unit 100. On the first side 1001, a first component

[0072] 1071 for fluid flow control and on the second side 1002 is a second component

[0073] 1072 for fluid flow control, which are connected via fluid channels to the pump 103, the environment 108 (or silencers 104), and a first pneumatic interface 1081, at which a first target pressure level 1051 can be provided, and a second pneumatic interface 1082, at which a second target pressure level 1052 can be provided, or storage containers 1021, 1022, which are arranged between the interfaces 1081, 1082 and the valves 1071, 1072. The first component 1071 for fluid flow control is formed in Fig. 1 by an electrically actuated 3 / 2-way valve, closed in the basic position, with a built-in mechanical spring for resetting. The second component 1072 for fluid flow control is formed in Fig. 1 by an electrically actuated 3 / 2-way valve which is open in the basic position and has a built-in mechanical spring for resetting.The two 3 / 2-way valves are configured to alternately connect the pump 103 via silencers 104 to the environment 108 of the pressure supply unit 100 and to pneumatic interfaces (connections 1081, 1082) at which the target pressure levels 1051, 1052 are provided. A system, in particular a microfluidic analysis system, is connected or connectable to the pressure supply unit 100 via the pneumatic interfaces 1081, 1082. The system can be supplied with the first target pressure level 1051 via the first connection 1081, and the system can be supplied with the second target pressure level 1052 via the second connection 1082.

[0074] The pump 103 can be designed, for example, as a diaphragm pump, which in most standard versions can be used for both vacuum and high pressure, as a blower, or as a compressor. The pump 103 sucks in a fluid, such as room air, via an upstream switching valve 1071, here a 3 / 2-way version. In the illustration, ambient pressure is sucked in during de-energized, open operation, so that the pump 103 functionally corresponds to the standard operating mode. The sucked-in fluid is compressed, and when the valve 1072, here also a 3 / 2-way version, on the high-pressure side (corresponds to the second side 1002), is not activated, pressure can be built up in the storage container 1002 and made available to the system, for example, the microfluidic analysis system.

[0075] If both valves 1071, 1072 are energized, the pump 103 operates as a standard vacuum pump by sucking fluid from the system via the low-pressure connection 1081, thereby creating a vacuum on the first side 1001 and discharging it to the environment 108 (here the connection to the environment 108 is provided with a silencer 104). A storage volume 1021, 1022 is provided on both sides of the pump 103 to increase the elasticity of the system and to cushion pressure peaks that may arise from possible switching of the valves 1071, 1072, from the starting of the pump 103, or from design-related pulsations of the latter. If only a pure spring effect via the storage tanks 1021, 1022 is desired, it is recommended to select a volume of 5-10 percent of the volume flow at the design operating point.

[0076] If the storage function is also to be addressed with the pressure supply unit 100, which is particularly important during shutdown operation, the volume should be designed depending on the components installed in the system, for example, a microfluidic analysis system connected to the pressure supply unit, and should be designed such that the system components can be supplied for their function from the storage units 1021, 1022 for 30-120 seconds when the pump 103 is not in operation. The pressure valves 1071, 1072 should generally be dimensioned such that the pressure loss across the valves 1071, 1072 is small compared to the working pressure, so as not to overly limit the efficiency of the pump 103. This should not exceed 5 percent of the system pressure and should be selected as minimal as possible.

[0077] For monitoring, but also to enable intermittent or shutdown operation of the pump in order to reduce its running time and thus minimize noise, pressure sensors 1062 and 1061 should be provided so that safety shutdowns as well as simple pressure controls can be implemented. In Fig. 1, pressure sensor units 1061, 1062 are arranged on both sides 1001, 1002 between valve 1071, 1072 and storage tank 1021, 1022 for monitoring the pressure, in particular for measuring the respective current pressure value. The described operating mode of (standard) pressure or vacuum generation is possible with the configuration shown and essentially corresponds to a dual-circuit design (ieTo provide each target pressure level, a pump 103 is used, in particular one pump each, which in the present case would correspond to two pumps (hence a dual-circuit design), which essentially share one pump 103 in the form of an X-shaped design. In this variant and operating mode, the power range of the pump 103 can be selected such that the pneumatic power corresponds to the maximum from both partial circuits (i.e., the first side 1011 and the second side 1002) and the target pressure levels 1051, 1052, for example, operation between 200 mbarA and 3000 mbarA system pressure, can be met.

[0078] With the proposed circuit, the pump 103 can also be operated in combination: If a pump with a sufficient power range is selected, the pump can, with the appropriate valve position (low-pressure valve 1071 energized; high-pressure valve 1072 de-energized), simultaneously generate low pressure as the first target pressure level 1051 and high pressure as the second target pressure level 1052 and make this available to the system, for example the microfluidic analysis system. If both current pressure ranges are not within the tolerance range around the target pressure value, i.e., they do not correspond to the respective target pressure level 1051, 1052, according to the control, for example, target pressure + / -100 mbar, the pump sucks fluid from the low-pressure area, i.e., the first side 1001, whereby it is vacuumized, and feeds the gas quantity directly into the high-pressure area, i.e., the second side 1002. If the target pressure level in one of the partial circuits, i.e.on one of the sides 1001, 1002, is reached, the corresponding valve 1071, 1072 on this side 1001, 1002 is switched to the environment in order to suck in the pumped fluid from the environment (vacuum side 1001) or discharge it into the environment (high pressure side 1002) in order not to exceed or fall below the control limits. If both target pressure levels 1051, 1052 are reached, the pump can, if it is operated as a continuous running pump, be connected to the environment in both the suction and pressure areas via the valves 1071, 1072, i.e. it can be operated in so-called idle mode. This is particularly useful if the proportion of this operating phase is very small in comparison to the other three operating modes (t<10 percent). Alternatively, the pump can then be operated in standby mode. Both pressure signals of the pressure sensor units 1061, 1062 should be included in the operation decision, since they are functionally linked logically OR.The following table shows the operating modes with the corresponding valve control states (O=de-energized;.

[0079] 1=energized) shown:

[0080] Fig. 2 shows a schematic structure of a pressure supply unit 100 according to an embodiment, which differs from the embodiment shown in Fig. 1 in particular in that check valves 1011, 1012 are used as components for controlling the fluid flow instead of switching valves 1071, 1072. This is a

[0081] Embodiment of a structurally simplified embodiment variant which enables further cost and complexity reductions and can be used in particular when the working pressure level, ie the first target pressure level 1051 and the second target pressure level 1052, is fixedly defined and should not be variably adjustable.

[0082] The pressure supply unit 100 comprises a pump 103, which is arranged between the first side 1001 and the second side 1002 of the pressure supply unit 100. On the first side 1001, a first component 1071 for fluid flow control is arranged and on the second side 1002, a second component 1072 for fluid flow control is arranged, which are connected via fluid channels to the pump 103, the environment 108 (or silencers 104) and a first pneumatic interface 1081, at which a first target pressure level 1051 can be provided and a second pneumatic interface 1082, at which a second target pressure level 1052 can be provided, or storage containers 1021, 1022, which are arranged between the interfaces 1081, 1082 and the valves 1071, 1072.

[0083] The setting of the target pressure level 1051, 1052 in the respective circuit, ie on the respective side 1001, 1002, is carried out in Fig. 2 via the opening pressure of the spring of the respective check valve 1011, 1012. Passive check valves 1011, 1012 are used here, the springs of which are dimensioned such that they:

[0084] • open on the vacuum side (here: first side 1001) when the current pressure level on the first side 1001 falls below a critical value (ie below the first target pressure level 1051, in particular target pressure value minus tolerance range), for example 300 mbar, and

[0085] • open on the high pressure side (here: second side 1002) if the current pressure level is above a critical value (ie above the second target pressure level, in particular target pressure value plus tolerance range), for example 2500 mbarA.

[0086] This embodiment of a pressure supply can also be used in standby mode if pressure sensors 1061, 1062 are used that allow control. Otherwise, such an embodiment is preferably suitable for continuous operation of the pump 103. With appropriate knowledge of the opening pressure of the check valves 1011, 1012, pressure monitoring (i.e., the pressure sensor units 1061, 1062 in Fig. 2, which are each arranged between the pump 103 and the storage tanks 1021, 1022) can be omitted, thereby enabling a further cost reduction. The pump 103 should then be operated in continuous operation in order to be able to maintain the target pressure levels 1051, 1052.

[0087] According to further embodiments, it is also possible to use a 3 / 2-way valve 1071 as the first component, wherein the first target pressure level 1051 is generated, as can be seen from the description of Fig.1, and to use a check valve 1012 as the second component, wherein the second target pressure level 1052 is generated, as can be seen from the description of Fig.2. Alternatively, a check valve 1011 can also be used as the first component, wherein the first target pressure level 1051 is generated, as can be seen from the description of Fig.2, and a 3 / 2-way valve 1072 can be used as the second component, wherein the second target pressure level 1052 is generated, as can be seen from the description of Fig.1. This can be particularly advantageous if one of the target pressure levels is to be adjustable and the other is fixed.

[0088] Fig. 3 shows a flowchart of a method 200 for providing the first target pressure 1051 and the second target pressure level 1052, in particular using a pressure supply unit 100 according to one of the embodiments described above, wherein the first target pressure level 1051 and the second target pressure level 1052 differ from one another.

[0089] The provision of the first target pressure level 1051 on the first side 1001, in particular at the first connection 1081 of the pressure supply unit 100, is carried out as follows: Depending on the first current pressure level 1053, a fluid flow is controlled as follows:

[0090] • Lowering 2010 the first current pressure level 1053 until reaching the first target pressure level 1051: If the first current pressure level 1053 is greater 2012 than the first target pressure level 1051, the first component 1071, 1011 controls the fluid flow such that the fluid, sucked in by the pump 103, flows from the first connection 1081 to the pump 103. As a result, the current pressure level 1053 at the first connection 1081 is lowered until it reaches the first target pressure level 1051.

[0091] • When the first target pressure level 1051 is reached 2011 or undershot: If the first current pressure level 1053 is less than or equal to 2013 the first target pressure level 1051, the first component 1071, 1011 controls the fluid flow in such a way that the fluid flow from the first connection 1081 to the pump 103 is interrupted and, in particular, the pump 103 is connected to the environment 108 instead, so that it sucks in fluid from the environment 108.

[0092] The provision of the second target pressure level 1052 on the second side 1002, in particular at the second connection 1082 of the pressure supply unit 100, is carried out as follows:

[0093] Depending on the second current pressure level 1052, a fluid flow is controlled as follows:

[0094] • Increasing 2020 the second current pressure level 1054 until reaching the second target pressure level 1052: If the second current pressure level 1054 on the second side 1002 is less than 2014 the second target pressure level 1052, the second component 1072, 1012 for fluid flow control controls the fluid flow such that the fluid is directed from the pump 103 to the second connection 1082

[0095] • When the second target pressure level 1052 is reached 2021 or falls below: If the current pressure level 1054 is greater than or equal to 2015 the second target pressure level 1052, the second component 1072, 1012 for fluid flow control controls the fluid flow in such a way that the fluid flow from the pump 103 to the second connection 1082 is interrupted and in particular the pump 103 is connected to the environment instead, so that it passes the fluid on to the environment.

[0096] Optionally, upon reaching 2011, 2021 the target pressure levels, instead of the previously described continuous operation in which the pump 103 draws fluid from the environment 108 on the first side 1001 and releases it to the environment on the second side 1002, a shutdown operation 203 of the pressure supply unit can begin, in which the pump 103 switches off. In particular, monitoring of the current pressure levels by pressure sensor units 1061, 1062 is advantageous here. If at least one of the target pressure levels 1051, 1052 is undershot, the pressure sensor units 1061, 1062 provide a control signal which is suitable for terminating the shutdown operation 203 by switching on the pump 103 or which is suitable for outputting a warning message to a user or to the system connected to the pressure supply unit 100.

[0097] Fig.4 shows a schematic representation of a microfluidic analysis system 300 for carrying out a sample analysis, comprising a test module unit 301 for receiving samples for a molecular diagnostic analysis, wherein the test module unit 301 in this embodiment comprises a test module 3001, comprising

[0098] • a pressure supply unit 100, as shown for example in Fig. 1 or Fig. 2, for providing the first target pressure level 1051 and the second target pressure level 1052,

[0099] • Peripheral components 305, 306, 307, 308 for performing a molecular diagnostic analysis and

[0100] • microfluidic elements 302, 303, 304 for sample transport in the test module unit 3000.

[0101] In this embodiment, the peripheral components include an optical unit

[0102] 305, which is configured, for example, to perform fluorescence measurements on the sample. The optical unit 305 can enable both illumination and optical excitation, as well as detection of an optical signal emanating from the sample. Furthermore, the peripheral components include a power supply unit 306, for example in the form of a power supply unit, which in particular provides alternating voltage, and a temperature control unit 307, which is configured to adjust a temperature in the test module unit, in particular by heating and / or cooling.

[0103] Furthermore, a mechanical unit 308 is also included among the peripheral components, which, among other things, enables the inclusion of a cartridge 309 (e.g., a clamping device for the cartridge 309), whereby the sample is introduced into the cartridge 309. The test module unit 301 is thus configured for sample inclusion. Furthermore, the mechanical unit 308 enables mechanical processing of the sample, such as rotation / centrifugation of the sample.

[0104] The microfluidic analysis system 300 can be operated as follows: The sample, in particular a liquid sample, is processed within the test module 3001 either partially or fully automatically by inserting the cartridge 309 into the microfluidic analysis system 300. The partially or fully automated processing of the sample can be carried out in particular by applying different pressure levels (the first and / or the second target pressure level 1051, 1052) to the test module 3001 via a suitable interface between the pressure supply unit 100 and the test module 3001.

[0105] First, the sample is introduced into the cartridge 309. In particular, the sample is present—at least partially—in liquid form, whereby the sample may in particular comprise a biological or medical substance, such as a body fluid (e.g., blood, saliva, etc.).

[0106] The cartridge 309, containing the sample, is inserted or placed into the test module 3001 and connected to it via the interfaces required for processing the sample in the cartridge 309. The sample can then be processed within the test module 3001. Depending on the selected procedure, the processing can include the following steps: purification, lysis, and thermal cycling of liquids, such as PCR procedures for detecting specific virus strains, etc. After processing, the cartridge 309 can be removed from the test module, and an analysis result of the sample can be output by the microfluidic analysis system, for example, via an optical display, and / or transmitted via a communication interface to another device, such as a printer or a mobile device.

[0107] The pressure supply unit 100 is connected or connectable to the test module unit 301 via the interface 302.

[0108] The microfluidic elements 302, 303, 304 of the test module unit 3000 can be controlled by the first target pressure level 1051 and / or the second target pressure level 1052. In this exemplary embodiment, the microfluidic elements comprise a pneumatic manifold 303 for distributing and controlling valves, and pressure vessels 304 (i.e., pneumatic accumulators 1021, 1022) for maintaining a minimum amount of compressed air as a reservoir, as well as an interface 302 to the outside. Alternatively or additionally, the pressure supply unit 100 can comprise the pneumatic accumulators 1021, 1022.

[0109] Fig. 5 shows a schematic sketch of a microfluidic analysis system 300, which forms a small parallel system 300', wherein the test module unit 301 comprises three test modules 3001, 3002, 3003. The test modules are constructed like the test module in Fig. 4. Each of the test modules 3001, 3002, 3003 is connected or connectable to the pressure supply unit 100 via its pneumatic interface 302. Thus, each of the test modules is supplied with the first target pressure level 1051 and the second target pressure level 1052 in parallel with the other test modules.

[0110] Fig. 6 shows a schematic sketch of a microfluidic analysis system 300, which forms a large parallel system 300', wherein the test module unit 301 comprises nine test modules 3001, 3002, 3003. The nine test modules are divided into three test module units 301, each comprising three test modules 3001, 3002, 3003. The pressure supply unit 100 supplies the three small parallel systems 300', which in this embodiment are constructed analogously to the small parallel system 300' shown in Fig. 5, with the first target pressure level 1051 and the second target pressure level 1052, respectively.

Claims

Claims 1. Pressure supply unit (100) for providing a first target pressure level (1051) and a second target pressure level (1051) for a microfluidic analysis system, wherein the first target pressure level (1051) and the second target pressure level (1052) differ from each other, comprising a pump (103) for sucking in a fluid, a first component (1011, 1071) for fluid flow control, which ■ is arranged on a first side (1001) of the pump (103), and ■ to enable fluid flow • from the first side (1001) of the pressure supply unit (100) to the pump (103), provided that a first current pressure level (1053) on the first side (1001) is greater than the first target pressure level (1051), and • from an environment (108) of the pressure supply unit (100) to the pump (103), provided that the first current pressure level (1053) on the first side (1001) is less than the first target pressure level (1051) or equal to the first target pressure level (1051), and is thus configured to provide the first target pressure level (1051) on the first side (1001) of the pressure supply unit (100), and a second component (1012, 1072) for fluid flow control, which ■ is arranged on a second side (1002) of the pump (103), and ■ to enable fluid flow • from the pump (103) to the second side (1002) of the pressure supply unit (100), provided that a second current pressure level (1054) on the second side (1002) is lower than the second target pressure level (1052), and • from the pump (103) to the environment (108) of the pressure supply unit (100), provided that a second current pressure level (1054) on the second side (1002) is greater than the second target pressure level (1052) or equal to the second target pressure level (1052), and is thus configured to provide the second target pressure level (1052) on the second side (1002) of the pressure supply unit (100). Pressure supply unit (100) according to claim 1, characterized in that the first component (1011, 1071) for fluid flow control is connected to a first storage volume (1021), in particular a first fluid reservoir, and / or the second component (1012, 1072) for fluid flow control is connected to a second storage volume (1022), in particular a second fluid reservoir. Pressure supply unit (100) according to one of the preceding claims, wherein the first target pressure level (1051) and the second target pressure level (1052) have a value of 200 mbarA, 3000 mbarA or a value in the range of 200 mbarA to 3000 mbarA system pressure.Pressure supply unit (100) according to one of the preceding claims, wherein a pressure sensor unit (1061, 1062) for pressure regulation and / or for controlling a safety shutdown is arranged on the first side (1001) and / or the second side (1002). Pressure supply unit (100) according to one of the preceding claims, wherein the first component (1011, 1071) for fluid flow control and the second component (1012) for fluid flow control are dimensioned such that a pressure loss across these components (1011, 1012, 1072) is less than or equal to 5% of the system pressure. Pressure supply unit (100) according to one of the preceding claims, wherein the first component (1011, 1071) and / or the second component (1012, 1072) for fluid flow control comprise a switching valve, in particular a 3 / 2-way valve.

7. Pressure supply unit (100) according to one of the preceding claims, wherein the first component (1011, 1071) and / or the second component (1012, 1072) comprise a check valve (1011, 1012) for controlling the fluid flow.

8. Method (200) for providing a first target pressure level (1051) and a second target pressure level (1052), in particular using a pressure supply unit (100) according to one of the preceding claims, wherein the first target pressure level (1051) and the second target pressure level (1052) differ from one another, comprising the following method steps: Providing (201) the first target pressure level (1051) on the first side (1001) of the pressure supply unit (100) by enabling a fluid flow ■ from the first side (1001) of the pressure supply unit (100) to the pump (103), provided that a first current pressure level (1053) on the first side (1001) is greater than the first target pressure level (1051), and ■ from an environment (108) of the pressure supply unit (100) to the pump (103), provided that the first current pressure level (1053) on the first side (1001) is less than the first target pressure level (1051) or equal to the first target pressure level (1051), Providing (202) the second target pressure level (1052) on the second side (1002) of the pressure supply unit (100) by forwarding the fluid ■ from the pump (103) to the second side (1002) of the pressure supply unit (100), provided that a second current pressure level (1054) on the second side (1002) is less than the second target pressure level (1052), and ■ from the pump (103) to the environment (108) of the pressure supply unit (100), provided that a second current pressure level (1054) on the second side (1002) is greater than the second target pressure level (1052) or equal to the second target pressure level (1052). The method according to claim 8, wherein upon reaching the first target pressure level (1051) on the first side (1001) and reaching the second target pressure level (1052) on the second side (1002), the pump (103) of the pressure supply unit (100) is switched off or the pump (103) is continued to operate, wherein during continued operation, the fluid is sucked in from the environment and passed to the environment by means of the pump (103). A microfluidic analysis system (300) for performing a sample analysis, comprising a test module unit (301) for receiving samples for a molecular diagnostic analysis, wherein the test module unit (301) comprises one test module (3001) or several test modules (3001, 3002, 3003), comprising • a pressure supply unit (100) according to one of the preceding claims for providing the first target pressure level (1051) and the second target pressure level (1052), • Peripheral components (305, 306, 307, 308) for performing a molecular diagnostic analysis and • microfluidic elements (302, 303, 304) for sample transport in the test module unit (301), - wherein the microfluidic elements (302, 303, 304) of the test module unit (301) can be controlled by means of the first target pressure level (1051) and / or the second target pressure level (1052), and - wherein the pressure supply unit (100) is connected or connectable to the test module unit (301) via an interface (302). The microfluidic analysis system (300) according to claim 10, wherein the microfluidic analysis system (300) comprises two or more test module units (301, 300') that can be controlled in parallel by the pressure supply unit (100). A control device configured to execute and / or control the steps of the method (200) according to one of claims 8 or 9 in corresponding units.

13. A computer program configured to execute and / or control the steps of the method (200) according to one of claims 8 or 9.

14. A machine-readable storage medium on which the computer program according to Claim 13 is stored.

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