Decontaminant solution and mehtod of using

Abstract

According to various aspects, a biocidal composition includes a mixture of at least two cationic surfactants; a component active against a microbiological organism; a nonionic surfactant; a chelant; a buffer; an antifoamer; and water.

Description

DECONTAMINANT SOLUTION AND MEHTOD OF USINGCLAIM OF PRIORITY AND CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] This application claims priority to U.S. Provisional Patent Application Serial No. 63 / 494,097, filed on April 4, 2023, entitled “DECONTAMINANT SOLUTION AND METHOD OF USING,” which is incorporated by reference herein in its entirety.BACKGROUND

[0002] A variety of disinfectants, including quaternary ammonium disinfectants, are on the market, and can be used for treatment of surfaces to remove or reduce microbials thereon. Many of these come as dilutable disinfectants, or as ready-to-use disinfectants.SUMMARY OF THE DISCLOSURE

[0003] According to various aspects, a biocidal composition includes a mixture of at least two cationic surfactants; a component active against a microbiological organism; a nonionic surfactant; a chelant; a buffer; an antifoamer; and water.DETAILED DESCRIPTION OF THE DISCLOSURE

[0004] Reference will now be made in detail to certain embodiments of the disclosed subject matter. While the disclosed subject matter will be described in conjunction with the enumerated claims, it will be understood that the exemplified subject matter is not intended to limit the claims to the disclosed subject matter.

[0005] Throughout this document, values expressed in a range format should be interpreted in a flexible manner to include not only the numerical values explicitly recited as the limits of the range, but also to include all the individual numerical values or sub-ranges encompassed within that range as if each numerical value and sub-range is explicitly recited. For example, a range of “about 0.1% to about 5%” or “about 0.1% to 5%” should be interpreted to include not just about 0.1% to about 5%, but also the individual values (e.g., 1%, 2%, 3%, and 4%) and the sub-ranges (e.g., 0.1% to 0.5%, 1.1% to 2.2%, 3.3% to 4.4%) within the indicated range. The statement “about X to Y” has the same meaning as “about X to about Y,” unlessindicated otherwise. Likewise, the statement “about X, Y, or about Z” has the same meaning as “about X, about Y, or about Z,” unless indicated otherwise.

[0006] In this document, the terms “a,” “an,” or “the” are used to include one or more than one unless the context clearly dictates otherwise. The term “or” is used to refer to a nonexclusive “or” unless otherwise indicated. The statement “at least one of A and B” or “at least one of A or B” has the same meaning as “A, B, or A and B.” In addition, it is to be understood that the phraseology or terminology employed herein, and not otherwise defined, is for the purpose of description only and not of limitation. Any use of section headings is intended to aid reading of the document and is not to be interpreted as limiting; information that is relevant to a section heading may occur within or outside of that particular section.

[0007] In the methods described herein, the acts can be carried out in any order without departing from the principles of the invention, except when a temporal or operational sequence is explicitly recited. Furthermore, specified acts can be carried out concurrently unless explicit claim language recites that they be carried out separately. For example, a claimed act of doing X and a claimed act of doing Y can be conducted simultaneously within a single operation, and the resulting process will fall within the literal scope of the claimed process.

[0008] The term “about” as used herein can allow for a degree of variability in a value or range, for example, within 10%, within 5%, or within 1% of a stated value or of a stated limit of a range, and includes the exact stated value or range. The term “substantially” as used herein refers to a majority of, or mostly, as in at least about 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, 99%, 99.5%, 99.9%, 99.99%, or at least about 99.999% or more, or 100%. The term “substantially free of’ as used herein can mean having none or having a trivial amount of, such that the amount of material present does not affect the material properties of the composition including the material, such that about 0 wt% to about 5 wt% of the composition is the material, or about 0 wt% to about 1 wt%, or about 5 wt% or less, or less than or equal to about 4.5 wt%, 4, 3.5, 3, 2.5, 2, 1.5, 1, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, 0.1, 0.01, or about 0.001 wt% or less, or about 0 wt%.

[0009] The term “organic group” as used herein refers to any carbon- containing functional group. Examples can include an oxygen-containing group such as an alkoxy group, aryloxy group, aralkyloxy group, oxo(carbonyl) group; a carboxyl group including a carboxylicacid, carboxylate, and a carboxylate ester; a sulfur- containing group such as an alkyl and aryl sulfide group; and other heteroatom- containing groups. Non-limiting examples of organic groups include OR, OOR, 0C(0)N(R)2, CN, CF3, OCF3, R, C(O), methylenedioxy, ethylenedioxy, N(R)2, SR, SOR, SO2R, SO2N(R)2, SO3R, C(O)R, C(O)C(O)R, C(O)CH2C(O)R, C(S)R, C(O)OR, OC(O)R, C(O)N(R)2, OC(O)N(R)2, C(S)N(R)2, (CH2)o-2N(R)C(0)R, (CH2)o-2N(R)N(R)2, N(R)N(R)C(O)R, N(R)N(R)C(O)OR, N(R)N(R)CON(R)2, N(R)SO2R, N(R)SO2N(R)2, N(R)C(O)OR, N(R)C(O)R, N(R)C(S)R, N(R)C(0)N(R)2, N(R)C(S)N(R)2, N(COR)COR, N(OR)R, C(=NH)N(R)2, C(O)N(OR)R, C(=NOR)R, and substituted or unsubstituted (Ci-Cioo)hydrocarbyl, wherein R can be hydrogen (in examples that include other carbon atoms) or a carbon-based moiety, and wherein the carbon-based moiety can be substituted or unsubstituted.

[0010] The term “substituted” as used herein in conjunction with a molecule or an organic group as defined herein refers to the state in which one or more hydrogen atoms contained therein are replaced by one or more non-hydrogen atoms. The term “functional group” or “substituent” as used herein refers to a group that can be or is substituted onto a molecule or onto an organic group. Examples of substituents or functional groups include, but are not limited to, a halogen (e.g., F, Cl, Br, and I); an oxygen atom in groups such as hydroxy groups, alkoxy groups, aryloxy groups, aralkyloxy groups, oxo(carbonyl) groups, carboxyl groups including carboxylic acids, carboxylates, and carboxylate esters; a sulfur atom in groups such as thiol groups, alkyl and aryl sulfide groups, sulfoxide groups, sulfone groups, sulfonyl groups, and sulfonamide groups; a nitrogen atom in groups such as amines, hydroxyamines, nitriles, nitro groups, N-oxides, hydrazides, azides, and enamines; and other heteroatoms in various other groups. Non-limiting examples of substituents that can be bonded to a substituted carbon (or other) atom include F, Cl, Br, I, OR, OC(O)N(R)2, CN, NO, NO2, ONO2, azido, CF3, OCF3, R, O (oxo), S (thiono), C(O), S(O), methylenedioxy, ethylenedioxy, N(R)2, SR, SOR, SO2R, SO2N(R)2, SO3R, C(O)R, C(O)C(O)R, C(O)CH2C(O)R, C(S)R, C(O)OR, OC(O)R, C(O)N(R)2, OC(O)N(R)2, C(S)N(R)2, (CH2)O-2N(R)C(0)R, (CH2)O-2N(R)N(R)2, N(R)N(R)C(O)R, N(R)N(R)C(O)OR, N(R)N(R)CON(R)2, N(R)SO2R, N(R)SO2N(R)2, N(R)C(O)OR, N(R)C(O)R, N(R)C(S)R, N(R)C(O)N(R)2, N(R)C(S)N(R)2, N(COR)COR, N(0R)R, C(=NH)N(R)2, C(O)N(OR)R, and C(=NOR)R, wherein R can be hydrogen or acarbon-based moiety; for example, R can be hydrogen, (Ci-Cioo)hydrocarbyl, alkyl, acyl, cycloalkyl, aryl, aralkyl, heterocyclyl, heteroaryl, or heteroarylalkyl; or wherein two R groups bonded to a nitrogen atom or to adjacent nitrogen atoms can together with the nitrogen atom or atoms form a heterocyclyl.

[0011] The term “alkyl” as used herein refers to straight chain and branched alkyl groups and cycloalkyl groups having from 1 to 40 carbon atoms, 1 to about 20 carbon atoms, 1 to 12 carbons or, in some embodiments, from 1 to 8 carbon atoms. Examples of straight chain alkyl groups include those with from 1 to 8 carbon atoms such as methyl, ethyl, n-propyl, n-butyl, n- pentyl, n-hexyl, n-heptyl, and n-octyl groups. Examples of branched alkyl groups include, but are not limited to, isopropyl, iso-butyl, sec-butyl, t-butyl, neopentyl, isopentyl, and 2,2- dimethylpropyl groups. As used herein, the term “alkyl” encompasses n-alkyl, isoalkyl, and anteisoalkyl groups as well as other branched chain forms of alkyl. Representative substituted alkyl groups can be substituted one or more times with any of the groups listed herein, for example, amino, hydroxy, cyano, carboxy, nitro, thio, alkoxy, and halogen groups.

[0012] The term “alkenyl” as used herein refers to straight and branched chain and cyclic alkyl groups as defined herein, except that at least one double bond exists between two carbon atoms. Thus, alkenyl groups have from 2 to 40 carbon atoms, or 2 to about 20 carbon atoms, or 2 to 12 carbon atoms or, in some embodiments, from 2 to 8 carbon atoms. Examples include, but are not limited to vinyl, -CH=CH(CH3), -CH=C(CH3)2, -C(CH3)=CH2, -C(CH3)=CH(CH3), - C(CH2CH3)=CH2, cyclohexenyl, cyclopentenyl, cyclohexadienyl, butadienyl, pentadienyl, and hexadienyl among others.

[0013] The term “alkynyl” as used herein refers to straight and branched chain alkyl groups, except that at least one triple bond exists between two carbon atoms. Thus, alkynyl groups have from 2 to 40 carbon atoms, 2 to about 20 carbon atoms, or from 2 to 12 carbons or, in some embodiments, from 2 to 8 carbon atoms. Examples include, but are not limited to - C =CH, -C=C(CH3), -C =C(CH2CH3), -CH2C =CH, -CH2C =C(CH3), and -C H2C =C(CH2CH3) among others.

[0014] The term “acyl” as used herein refers to a group containing a carbonyl moiety wherein the group is bonded via the carbonyl carbon atom. The carbonyl carbon atom is bonded to a hydrogen forming a “formyl” group or is bonded to another carbon atom, which can be partof an alkyl, aryl, aralkyl cycloalkyl, cycloalkylalkyl, heterocyclyl, heterocyclylalkyl, heteroaryl, heteroarylalkyl group or the like. An acyl group can include 0 to about 12, 0 to about 20, or 0 to about 40 additional carbon atoms bonded to the carbonyl group. An acyl group can include double or triple bonds within the meaning herein. An acryloyl group is an example of an acyl group. An acyl group can also include heteroatoms within the meaning herein. A nicotinoyl group (pyridy 1-3 -carbonyl) is an example of an acyl group within the meaning herein. Other examples include acetyl, benzoyl, phenylacetyl, pyridylacetyl, cinnamoyl, and acryloyl groups and the like. When the group containing the carbon atom that is bonded to the carbonyl carbon atom contains a halogen, the group is termed a “haloacyl” group. An example is a trifluoroacetyl group.

[0015] The term “cycloalkyl” as used herein refers to cyclic alkyl groups such as, but not limited to, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, and cyclooctyl groups. In some embodiments, the cycloalkyl group can have 3 to about 8-12 ring members, whereas in other embodiments the number of ring carbon atoms range from 3 to 4, 5, 6, or 7. Cycloalkyl groups further include polycyclic cycloalkyl groups such as, but not limited to, norbornyl, adamantyl, bornyl, camphenyl, isocamphenyl, and carenyl groups, and fused rings such as, but not limited to, decalinyl, and the like. Cycloalkyl groups also include rings that are substituted with straight or branched chain alkyl groups as defined herein. Representative substituted cycloalkyl groups can be mono- substituted or substituted more than once, such as, but not limited to, 2,2-, 2,3-, 2,4- 2,5- or 2,6-disubstituted cyclohexyl groups or mono-, di- or trisubstituted norbornyl or cycloheptyl groups, which can be substituted with, for example, amino, hydroxy, cyano, carboxy, nitro, thio, alkoxy, and halogen groups. The term “cycloalkenyl” alone or in combination denotes a cyclic alkenyl group.

[0016] The term “aryl” as used herein refers to cyclic aromatic hydrocarbon groups that do not contain heteroatoms in the ring. Thus aryl groups include, but are not limited to, phenyl, azulenyl, heptalenyl, biphenyl, indacenyl, fluorenyl, phenanthrenyl, triphenylenyl, pyrenyl, naphthacenyl, chrysenyl, biphenylenyl, anthracenyl, and naphthyl groups. In some embodiments, aryl groups contain about 6 to about 14 carbons in the ring portions of the groups. Aryl groups can be unsubstituted or substituted, as defined herein. Representative substituted aryl groups can be mono-substituted or substituted more than once, such as, but not limited to, aphenyl group substituted at any one or more of 2-, 3-, 4-, 5-, or 6-positions of the phenyl ring, or a naphthyl group substituted at any one or more of 2- to 8-positions thereof.

[0017] The term “aralkyl” as used herein refers to alkyl groups as defined herein in which a hydrogen or carbon bond of an alkyl group is replaced with a bond to an aryl group as defined herein. Representative aralkyl groups include benzyl and phenylethyl groups and fused (cycloalkylaryl)alkyl groups such as 4-ethyl-indanyl. Aralkenyl groups are alkenyl groups as defined herein in which a hydrogen or carbon bond of an alkyl group is replaced with a bond to an aryl group as defined herein.

[0018] The term “heterocyclyl” as used herein refers to aromatic and non-aromatic ring compounds containing three or more ring members, of which one or more is a heteroatom such as, but not limited to, N, O, and S.

[0019] The term “alkoxy” as used herein refers to an oxygen atom connected to an alkyl group, including a cycloalkyl group, as are defined herein. Examples of linear alkoxy groups include but are not limited to methoxy, ethoxy, propoxy, butoxy, pentyloxy, hexyloxy, and the like. Examples of branched alkoxy include but are not limited to isopropoxy, sec-butoxy, tertbutoxy, isopentyloxy, isohexyloxy, and the like. Examples of cyclic alkoxy include but are not limited to cyclopropyloxy, cyclobutyloxy, cyclopentyloxy, cyclohexyloxy, and the like. An alkoxy group can include about 1 to about 12, about 1 to about 20, or about 1 to about 40 carbon atoms bonded to the oxygen atom, and can further include double or triple bonds, and can also include heteroatoms. For example, an allyloxy group or a methoxy ethoxy group is also an alkoxy group within the meaning herein, as is a methylenedioxy group in a context where two adjacent atoms of a structure are substituted therewith.

[0020] The term “amine” as used herein refers to primary, secondary, and tertiary amines having, e.g., the formula N(group)s wherein each group can independently be H or non-H, such as alkyl, aryl, and the like. Amines include but are not limited to R-NH2, for example, alkylamines, arylamines, alkylarylamines; R2NH wherein each R is independently selected, such as dialkylamines, diarylamines, aralkylamines, heterocyclylamines and the like; and R3N wherein each R is independently selected, such as trialkylamines, dialkylarylamines, alkyldiarylamines, triarylamines, and the like. The term “amine” also includes ammonium ions as used herein.

[0021] The term “amino group” as used herein refers to a substituent of the form -NH2, - NHR, -NR2, -NR3+, wherein each R is independently selected, and protonated forms of each, except for -NR3+, which cannot be protonated. Accordingly, any compound substituted with an amino group can be viewed as an amine. An “amino group” within the meaning herein can be a primary, secondary, tertiary, or quaternary amino group. An “alkylamino” group includes a monoalkylamino, dialkylamino, and trialkylamino group.

[0022] The terms “halo,” “halogen,” or “halide” group, as used herein, by themselves or as part of another substituent, mean, unless otherwise stated, a fluorine, chlorine, bromine, or iodine atom.

[0023] The term “haloalkyl” group, as used herein, includes mono-halo alkyl groups, poly-halo alkyl groups wherein all halo atoms can be the same or different, and per-halo alkyl groups, wherein all hydrogen atoms are replaced by halogen atoms, such as fluoro. Examples of haloalkyl include trifluoromethyl, 1 , 1 -di chloroethyl, 1 ,2-dichloroethyl, l,3-dibromo-3,3- difluoropropyl, perfluorobutyl, and the like.

[0024] The term “hydrocarbon” or “hydrocarbyl” as used herein refers to a molecule or functional group that includes carbon and hydrogen atoms. The term can also refer to a molecule or functional group that normally includes both carbon and hydrogen atoms but wherein all the hydrogen atoms are substituted with other functional groups. The term “hydrocarbyl” refers to a functional group derived from a straight chain, branched, or cyclic hydrocarbon, and can be alkyl, alkenyl, alkynyl, aryl, cycloalkyl, acyl, or any combination thereof. Hydrocarbyl groups can be shown as (Ca-Cb)hydrocarbyl, wherein a and b are integers and mean having any of a to b number of carbon atoms. For example, (Ci-C4)hydrocarbyl means the hydrocarbyl group can be methyl (Ci), ethyl (C2), propyl (C3), or butyl (C4), and (Co-Cb)hydrocarbyl means in certain embodiments there is no hydrocarbyl group. A hydrocarbylene group is a diradical hydrocarbon, e.g., a hydrocarbon that is bonded at two locations.

[0025] The polymers described herein can terminate in any suitable way. In some embodiments, the polymers can terminate with an end group that is independently chosen from a suitable polymerization initiator, -H, -OH, a substituted or unsubstituted (Ci-C2o)hydrocarbyl (e.g., (Ci-Cio)alkyl or (Ce-C2o)aryl) interrupted with 0, 1, 2, or 3 groups independently selectedfrom -O-, substituted or unsubstituted -NH-, and -S-, a poly(substituted or unsubstituted (Ci- C2o)hydrocarbyloxy), and a poly(substituted or unsubstituted (Ci-C2o)hydrocarbylamino).

[0026] According to various aspects, a biocidal composition can be used against many different microbiological organisms. For example, the biocidal composition can be effective or active against bacteria, fungus, yeast, a virus, or a mixture thereof. As specific examples, the biocidal composition can be effective or active against P. aeruginosa, E. hirae, S. aureus, C. albicans, M. terrae, A. brasiliensis, Adenovirus, modified vaccinia Ankara, or a mixture thereof. With respect to a bacteria or virus, a LogR can be greater than or equal to 4 or greater than or equal to 5.

[0027] The biocidal composition includes a mixture of at least two cationic surfactants; a component active against a microbiological organism; a nonionic surfactant; a chelant; a buffer; an antifoamer; and water.

[0028] The at least two cationic surfactants include benzalkonium chloride and didecyldimethylammonium chloride. Benzalkonium chloride is a type of cationic surfactant. It is an organic salt classified as a quaternary ammonium compound. ADBACs have three main categories of use: as a biocide, a cationic surfactant, and a phase transfer agent. ADBACs are a mixture of alkylbenzyldimethylammonium chlorides, in which the alkyl group has various even-numbered alkyl chain lengths. Didecyldimethyl ammonium chloride (DDAC), is a quaternary ammonium compound often used as an antiseptic or disinfectant. DDAC is a straight chain polymer having a chemical formula of C22H48CIN, a molar mass of 362.08 g / mol, and a density of 0.87 g / cm3at 20 °C. DDAC acts as an antimicrobial agent by disrupting intermolecular interactions and dissociating lipid bilayers. DDAC is a broad spectrum biocidal against bacteria and fungi.

[0029] The benzalkonium chloride can be in a range of from about 12 wt% to about 22 wt% of the dry weight of the biocidal composition, about 15 wt% to about 20 wt%, less than, equal to, or greater than about 12 wt%, 13, 14, 15, 16, 17, 18, 19, 20, 21, or about 22 wt%. DDAC can be present in a range of from about 0.5 wt% to about 7 wt% of the biocidal composition, about 2.5 wt% to about 3.5 wt%, less than, equal to, or greater than about 0.5 wt%, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, or about 7 wt%. In total, the at least two cationic surfactants together range from about 25 wt% to about 60 wt% of the biocidal composition,about 30 wt% to about 45 wt%, less than, equal to, or greater than about 25 wt%, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, and 60 wt%.

[0030] The biocidal composition further includes a non-ionic surfactant comprises. A particular example of a suitable non-ionic surfactant includes an alkyl polyglycoside. Alkyl polyglycosides (APGs) are a class of non-ionic surfactants widely used in a variety of cosmetic, household, and industrial applications. Biodegradable and plant-derived from sugars, these surfactants are usually glucose derivatives, and fatty alcohols. The raw materials are typically starch and fat, and the final products are typically complex mixtures of compounds with different sugars comprising the hydrophilic end and alkyl groups of variable length comprising the hydrophobic end. When derived from glucose, they are known as alkyl polyglucosides.

[0031] The non-ionic surfactant is in a range of from about 4 wt% to about 16 wt% of the biocidal composition, about 5 wt% to about 10 wt%, less than, equal to, or greater than about 4 wt%, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, or about 16 wt%.

[0032] The biocidal composition includes a component active against a microbiological organism. This component is in a range of from about 0.1 wt% to about 0.8 wt% of the biocidal composition, about 0.2 wt% to about 0.4 wt%, less than, equal to, or greater than about 0.1 wt%, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.55, 0.60, 0.65, 0.7, 0.75, or about 0.8 wt%. An example of a broadly applicable component active against a microbiological organism is chlorohexidine digluconate. Chlorohexidine digluconate (CHG) is used in disinfectants (disinfection of the skin and hands), cosmetics (additive to creams, toothpaste, deodorants, and antiperspirants), and pharmaceutical products (preservative in eye drops, active substance in wound dressings and antiseptic mouthwashes). CHG is active against Gram-positive and Gramnegative organisms, facultative anaerobes, aerobes, and yeasts. It is particularly effective or active against Gram-positive bacteria (in concentrations > 1 pg / L). At physiologic pH, chlorhexidine salts dissociate and release the positively charged chlorhexidine cation. The bactericidal effect is a result of the binding of this cationic molecule to negatively charged bacterial cell walls. At low concentrations of chlorhexidine, this results in a bacteriostatic effect; at high concentrations, membrane disruption results in cell death. Unexpectedly, it was foundthat there is a synergistic effect in effectiveness against microorganisms when CHG and benzalkonium chloride are combined (along with the other components described herein).

[0033] The chelant can be present in a range of from about 0.05 wt% to about 1.5 wt% of the biocidal composition, about 0.2 wt% to about 0.7 wt%, less than, equal to, or greater than about 0.05 wt%, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.55, 0.6, 0.65, 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 1, 1.05, 1.1, 1.15, 1.2, 1.25, 1.3, 1.35, 1.4, 1.45, or about 1.5 wt%. Examples of suitable chelants can include tetrasodium glutamate diacetate (GLDA), ethylenediaminetetraacetic acid (EDTA), ethylenediamine-N, N’ -disuccinic acid (EDDS), NT A, EDDS, IDS, methylglycinediacetic acid (MGDA), L-glutamic acid N,N-diacetic acid (GLDA), ethylenediamine-N, N-diglutaric acid (EDDG), ethylenediamine-N, N’ -dimalonic acid (EDDM), 3-hydroxy-2,2-iminodisuccinic acid (HIDS), 2-hydroxy ethyliminodiacetic acid (HEID A), methylglycine N,N-diacetic acid trisodium salt (MGDA), or a mixture thereof.

[0034] The biocidal composition further includes a stabilizer. In some examples, the stabilizer can be l-hydroxyethylidene-l,l,-diphosphonic acid. In further examples, the stabilizer can be a polymeric stabilizer that is selected from a phosphino polycarboxylic acid, or salt thereof. In some examples, the phosphino polycarboxylic acid has formula (I)(i)wherein R2isR3isR4, at each occurrence, is independently hydrogen or Ci-4alkyl; and m and n are each independently an integer, where m+n is an integer from 30 to 60. In some examples, R4is hydrogen. In some examples, the phosphino poly carboxy lie acid has a molecular weight of 3300- 3900 g / mol.

[0035] In some examples, the one or more polymeric stabilizers is selected from a polymer, or salt thereof, with molecular weight of 3000 to 15,000 g / mol, the polymer being derived from a plurality of monomer units of each ofIn some examples, the polymer is derived from a plurality of monomer units of each ofThe polymeric stabilizers can include the specified monomer units.

[0036] In some examples, the one or more polymeric stabilizers is selected from a polymer, or salt thereof, with molecular weight of 3000 to 15,000 g / mol, the polymer being derived from a plurality of monomer units of each ofIn some examples, the polymer is derived from a plurality of monomer units of each ofThe polymeric stabilizers can include the specified monomer units.

[0037] In some examples, the peroxide composition is stabilized with 0.1-1500 ppm of the one or more polymeric stabilizers. The use of the polymeric stabilizer system herein does not preclude or restrict the presence of other known stabilizers for peroxygens. Stabilized peroxyacid compositions of the disclosure may include additional stabilizers or additives, such as a phosphate, a stannate, or a chelant. Compounds such as polycarboxylic acids, including for example dipicolinic acid, ethylenediaminetetraacetic acid or citric acid, soluble salts of phosphates which may take the form of simple monomeric species, or of condensed linear polyphosphates or cyclic metaphosphates. Further known stabilizers which may be included are organophosphonates, which molecules may additionally contain other functional groups such as hydroxy or amino. These are exemplified in compounds such as 1 -hydroxy ethylidene- 1,1- diphosphonic acid and poly(methyleneamino)-phosphonic acids such as aminotri(methylenephosphonic acid), and diethylenetriaminepenta(methylenephosphonic acid).

[0038] Useful stannates include an alkali metal stannate, particularly sodium stannate (Na2(Sn(OH)e). Stannates further include stannic chloride, stannic oxide, stannic bromide, stannic chromate, stannic iodide, stannic sulfide, tin dichloride bis(2,4-pentanedionate), tin phthalocyanine dichloride, tin acetate, tin t-butoxide, di-n-butyl tin(IV) dichloride, tin methacrylate, tin fluoride, tin bromide, stannic phosphide, stannous chloride, stannous fluoride, stannous pyrophosphate, sodium stannate, stannous 2- ethylhexoate, stannous bromide, stannous chromate, stannous fluoride, stannous methanesulfonate, stannous oxalate, stannous oxide, stannous sulfate, stannous sulfide, barium stannate, calcium stannate, copper(II) stannate, leadstannate dihydrate, zinc stannate, sodium stannate, potassium stannate trihydrate, strontium stannate, cobalt(II) stannate dihydrate, sodium trifluorostannate, ammonium hexachlorostannate, and lithium hexafluorostannate.

[0039] Another optional component of the stabilized peroxy acid compositions is the soluble salt of a phosphate. The phosphate salt can take the form of the simple monomeric species, or of the condensed linear polyphosphate, or cyclic polyphosphate(metaphosphate). The monomeric phosphate salts are of the general formula, MnHqPC , (in which q=0, 1, or 2; n=l, 2, or 3; n+q=3). Here M can be one or more monovalent cations selected from the following: Li, Na, K, NH4, NR4 (where R represents an alkyl chain containing 1 to 5 C atoms). The polyphosphates have the general formula, Mn+2PnO3n+i where n=2 to 8, and M can be chosen from Li, Na, K, NH4, NR4 where R represents an alkyl chain containing 1 to 5 C atoms). The cyclic polyphosphates have the general formula MnPnChn where n=3 to 8 and M can be chosen from Li, Na, K, NH4, NR4 where R represents a linear or branched alkyl group containing 1 to 5 C atoms).

[0040] The above may be optionally introduced into the stabilizer system in their acid form. Exemplary phosphates include pyrophosphoric acid and metaphorphoric acid and their salts, e.g., sodium salts

[0041] Any phosphonic acid based chelant can optionally be used, such as amino trimethylene phosphonic acid (ATMP), 2-phosphonobutane-l,2,4-tricarboxylic acid (PBTCA), N-sulfonic amino dimethylene phosphonic acid (SADP), methylamine dimethylene phosphonic acid (MADMP), glycine dimethyl phosphonic acid (GDMP), 2-hydroxyphosphonocarboxylic acid (HPAA), polyhydric alcohol phosphate ester (PAPE), 1 -hydroxy ethylidene- 1,1- diphosphonic acid (HEDP), 1 -aminoethane- 1,1-diphosphonic acid, amino tri(methylenephosphonic acid) (ATMP), ethylene diamine tetra(methylenephosphonic acid), hexamethylene diamine tetra(methylenephosphonic acid), diethylenetriamine penta(methylenephosphonic acid) (DTPMP), diethylenetriamine hexa(methylenephosphonic acid), and 1 -aminoalkane- 1,1-diphosphonic acids such as morpholinomethane diphosphonic acid, N,N-dimethyl aminodimethyl diphosphonic acid, aminomethyl diphosphonic acid, or salts thereof, for example sodium salts.

[0042] Yet further stabilizers to be contemplated are free radical scavengers. Useful radical scavengers include pyridine carboxylic acids, such as 2,6-pyridine dicarboxylic acid and picolinic acid. Scavengers may be selected from amongst phenols, polyols, or thiols. Suitable scavengers are those selected from phenols which satisfy the general formula (II)in which R represents at least one substituent selected from alkyl, ether, hydroxyl, carboxylic acid, and aliphatic carboxylic acid ester groups. Normally R in (II), represents from 1 to 4 substituents. One suitable sub-class of phenolic scavengers polyhydroxybenzoic acid or alkyl ester derivatives thereof, the benzene nucleus optionally being further substituted by one or more alkyl substituents. Normally not more than a single carboxylic acid / ester substituent is present. Included within that sub-class are the dihydroxybenzoic acids, gallic acid, pyrogallic acid and ester derivatives thereof. Other suitable sub-classes comprise polyhydroxy alkylbenzenes or ether derivatives thereof. Representatives of that subgroup include alkylresorcinols and alkylhydroquinones. Further suitable radical scavengers are substituted polyaryl compounds including those where one or more of the aryl rings is substituted with a heteroatom. A suitable example from this group is 8-hydroxyquinoline.

[0043] Also to be contemplated as phosphorus containing salts are organophosphonates which may be introduced as a soluble salt or as the parent acid. Compounds which may be contemplated include, ethylphosphonic acid, propylphosphonic acid, butylphosphonic acid, t- butylphosphonic acid, or phenylphosphonic acid. Additionally the phosphonic acid molecules can contain other functional groups such as hydroxy or amino. These are exemplified in compounds such; as l-hydroxyethylidene-l,l-diphosphonic acid, and poly(methyleneamino) phosphonic acids such as aminotri(methylene phosphonic acid), and diethylenetriaminepenta(methylenephosphonic acid).

[0044] Further additives which may be contemplated are sulphuric acid, or soluble salts thereof. Organosulphonic acids such as decylsulphonic acid, dodecylsulphonic acid, toluenesulphonic acid and methylsulphonic acid, as well as their salts, may also be introduced. Stannate may be employed at a concentration of up to 15% of the total stabilizer composition, or at a concentration of between 8 to 12%

[0045] It is to be understood that the composition of the disclosure can include mixtures of one or more polymeric stabilizers, two or more of at least one protective colloid at least one radical scavenger and at least one phosphonic acid based chelant.

[0046] A particularly suitable stabilizer is a 2,6-diphenyl-4,8diazoadamantan-l-one or derivative thereof. Details of this stabilizer can be found in published PCT patent application no. WO 2008 / 078208, the contents of which are hereby incorporated by reference. The stabilizer can be present in a range of from about 0.005 wt% to about 0.05 wt% of the biocidal composition, about 0.008 wt% to about 0.02 wt%, less than, equal to, or greater than about 0.005 wt%, 0.006, 0.007, 0.008, 0.009, 0.01, 0.02, 0.03, 0.04, or about 0.05 wt%.

[0047] A buffer can be in a range of from about 1 wt% to about 5 wt% of the biocidal composition, about 2 wt% to about 4 wt%, less than, equal to, or greater than about 1 wt%, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, or about 5 wt%. The buffer can include a mixture of Na2HPO4 and KH2PO4. Within the mixture, the Na2HPO4 is in a range of from about 1 wt% to about 3 wt% of the biocidal composition, about 1.5 wt% to about 2.5 wt%, less than, equal to, or greater than about 1 wt%, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9 or about 3 wt%. Within mixture, the KH2PO4 is in a range of from about 0.5 wt% to about 1.5 wt% of the biocidal composition, about 0.7 wt% to about 1.3 wt%, less than, equal to, or greater than about 0.5 wt%, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, or 1.5 wt% of the biocidal composition.

[0048] The biocidal composition includes an antifoamer. An antifoamer is a chemical additive that reduces and hinders the formation of foam in industrial process liquids. The terms antifoam agent and defoamer are often used interchangeably. Strictly speaking, defoamers eliminate existing foam and antifoamers prevent the formation of further foam. Commonly used agents are insoluble oils, polydimethylsiloxanes and other silicones, certain alcohols, stearates and glycols. The additive is used to prevent formation of foam or is added to break a foam already formed. In industrial processes, foams pose serious problems. They cause defects on surface coatings and prevent the efficient filling of containers. A variety of chemical formulae are available to prevent formation of foams. Silicone-based defoamers are polymers with silicon backbones. These might be delivered as an oil or a water-based emulsion. The silicone compound consists of a hydrophobic silica dispersed in a silicone oil. Emulsifiers are added to ensure that the silicone spreads fast and well in the foaming medium. The siliconecompound might also contain silicone glycols and other modified silicone fluids. In specific examples, the antifoamer includes an active silicone composition.

[0049] The enzyme component can include one enzyme or a mixture of enzymes. For example, the enzyme component can include up to two enzymes, up to three enzymes, up to four enzymes, up to five enzymes, and so on. In total, the enzyme component is in a range of from about 1 wt% to about 5 wt% of the biocidal composition, about 1 wt% to about 2.5 wt%, less than, equal to, or greater than about 1 wt%, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, or about 5 wt%. The classification of the enzymes can be any enzyme class or species that is capable of biocidal activity. A particularly suitable class incudes a protease. Further examples inlcude a cellulase or amylase. These enzymes can selectively breakdown polymers (e.g., into subunit monomers).

[0050] As stated above, the biocidal composition can be effective or active against bacteria, fungus, yeast, a virus, or a mixture thereof. As used herein, the term “bacteria” generally refers to gram-positive and gram-negative bacteria. Gram-positive bacteria include, but are not limited to, mycobacteria. Mycobacteria, in turn, include, but are not limited to, M. africanum, M. avium, M. bovis, M. chelonei, M. farcinogenes, M. jlavum, M. fortuitum, M. haemophilum, M. intracellulare, M. kansasii, M. leprae, M. lepraemurium, M. marinum, M. microti, M. parafortuitum, M. paratuberculosis, M. phlei, M. scrofulaceum, M. senegalense, M. simiae, M. smegmatis, M. thermoresistibile, M. tuberculosis, M. ulcerans, and AT. xenopi. Other gram-positive bacteria include, but are not limited to, gram-positive cocci including Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), Group A Streptococci, Group B Streptococci, Group C Streptococci, Group G Streptococci, and vancomycin resistant Enterococci (VRE). Other gram-positive bacteria include Listeria spp.(e.g, Listeria monocytogenes, Listeria innocua), Clostridium spp. (e.g., Clostridium perfringens and Clostridium botulinum), and Bacillus cereus.

[0051] Gram-negative bacteria include, but are not limited to, Pseudomonas aeruginosa, Pseudomonas spp., Serratia marcescens, E. coli, Salmonella spp., Campylobacter jejuni, Shigella, and Vibrio spp. The bacteria can be at least one of Escherichia coli, Listeria innocua, Listeria spp. Salmonella enterica, Salmonella spp., Mycobacterium parafortuitum, Saccharomyces cerevisiae, Pseudomonas aeruginosa, Pseudomonas spp. , Serratia marcescens, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcusepidermitis, methicillin-resistant Staphylococcus epidermidis (MRSE), Propionibacterium acnes, Group A Streptococci, Group B, Streptococci, Group C Streptococci, Group G Streptococci, vancomycin resistant Enterococci (VRE), and Acinetobacter baumannii.

[0052] The biocidal composition described herein can be prepared as a concentrated composition. The concentrated composition can be diluted prior to use. For example, the concentrated composition can still demonstrate biocidal activity when it is present in a diluted formula in a range of from about 0.1 wt% to about 10 wt%, about 0.2 wt% to about 5 wt%, less than, equal to, or greater than about 0.1 wt%, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, or about 10 wt%.

[0053] The biocidal composition described herein can be produced by mixing any of combination of the afore mentioned components together.

[0054] In specific examples, the composition can effectively disinfectant a substrate. In further specific examples, the composition can effectively disinfectant the surface of a substrate. In additional specific examples, the composition can effectively decontaminate a substrate. In further specific examples, the composition can effectively decontaminate the surface of a substrate.

[0055] The composition of the present disclosure can be formulated for application, depending upon the user's preference as well as the ultimate application of the composition. For example, the composition can be formulated for use in a sprayable composition, atomized liquid sprayer, or liquid applicator. Such formulations can include at least one of a spray bottle, motorized sprayer, wipe, cloth, sponge, non-woven fabric, and woven fabric. Such formulations may be particularly suitable for applying the composition to a surface of a hospital, physician's office, medical clinic, medical facility, dental office, dental facility, airport, school, pet store, zoo, children's day care, elderly nursing home, museum, movie theatre, athletic facility, sporting arena, gymnasium, rest room, bathroom, shopping center, amusement park, church, synagogue, mosque, temple, restaurant, food processing facility, food manufacturing facility, pharmaceutical company, hot-tub, sauna, and / or clean room. Such liquid formulations may be particularly suitable for applying the composition to metal, plastic, natural rubber, synthetic rubber, glass, stone, , fiberglass,.

[0056] In specific examples, the composition of the present disclosure can be non-toxic. The term “non-toxic” refers to a substance that has a relatively low degree to which it can damage a living or non-living organism. Toxicity can refer to the effect on a whole organism, such as an animal, bacterium, or plant, as well as the effect on a substructure of the organism, such as a cell (cytotoxicity) or an organ (organotoxicity), such as the liver (hepatotoxicity). A central concept of toxicology is that effects are dose-dependent; even water can lead to water intoxication when taken in large enough doses, whereas for even a very toxic substance such as snake venom there is a dose below which there is no detectable toxic effect. Having the composition be relatively non-toxic will allow a wider range of users be able to safely handle the composition, without serious safety concerns or risks.

[0057] The present disclosure also provides for a kit that includes: (a) an enclosed container that includes a removable closure; (b) the composition of the present disclosure as described herein, which is located inside the enclosed container; and (c) printed indicia located on the enclosed container.

[0058] In specific examples, the enclosed container can be opaque. In additional specific examples, the enclosed container can be manufactured from high density polyethylene (HDPE), thereby providing the requisite opacity.Examples

[0059] Various embodiments of the present disclosure can be better understood by reference to the following Examples which are offered by way of illustration. The present disclosure is not limited to the Examples given herein.Example 1

[0060] Various active components or mixtures of active components were tested against various target microorgamisms for their effectiveness. The active components tested include BAC: Benzalkonium Chloride - CAS#: 63449-41-2, CLX: Chlorhexidine Digluconate - CAS#: 18472-51-0, and APG: Alkyl PolyGlucoside - CAS#: 68515-73-1. As shown in Table 1 the combinaiton of BAC, CLX, and APG yielded the best results, thus demonstrating a synergistic effect of combining BAC and CLX or a combination of BAC, CLX, and APG.Table 1:SUBSTITUTE SHEET (RULE 26)

[0061] The effectiveness of BAC was further tested. As shown in Table 2, The Benzalkonium Chloride alone, as active ingredient, is not able to reach a LogR > 5 when its concentration in the dosed solution is near 800 ppm. Increasing the Benzalkonium Chloride concentration to 1000 ppm improve its performance but is still not able to reach a LogR > 5. This active ingredient started to reach the target LogR when its concentration is around 1600 ppm. Table 2 shows that The Benzalkonium Chloride alone as active ingredient is able to reach a LogR > 5 on P. aeruginosa (EN13727, suspension) when its concentration is around 1600 ppm. This high concentration of a concentration means that it could only be effective as a really concentrated formula or would need to be present at a high dosage.Table 2SUBSTITUTE SHEET (RULE 26)

[0062] The synergistic effect of a mixture of BAC and CLX is shown in Table 3. In Table 3, Four formulations containing Benzalkonium Chloride and Chlorhexidine Digluconate in a ratio BAC:CLX = 46:1 were tested. As demonstrated, the Benzalkonium Chloride alone, as active ingredient, is not able to reach a LogR > 5 when its concentration in the dosed solution is near 800 ppm. If the benzalkonium chloride is added to chlorhexidine digluconate (at a ratio of BAC:CLX = 46:1) the performance, dramatically increase. This effect allows for the decrease of the concentration of active substance up to 400-500 ppm (as sum of Benzalkonium Chloride and Chlorhexidine Digluconate). Benefits associated with this include being able to use minor dosage of the concentrated product.SUBSTITUTE SHEET (RULE 26)Table 3SUBSTITUTE SHEET (RULE 26)

[0063] The terms and expressions that have been employed are used as terms of description and not of limitation, and there is no intention in the use of such terms and expressions of excluding any equivalents of the features shown and described or portions thereof, but it is recognized that various modifications are possible within the scope of the embodiments of the present disclosure. Thus, it should be understood that although the present disclosure has been specifically disclosed by specific embodiments and optional features, modification and variation of the concepts herein disclosed may be resorted to by those of ordinary skill in the art, and that such modifications and variations are considered to be within the scope of embodiments of the present disclosure.Exemplary Embodiments.

[0064] The following exemplary embodiments are provided, the numbering of which is not to be construed as designating levels of importance:

[0065] Aspect 1 provides a biocidal composition comprising: a mixture of at least two cationic surfactants; a component active against a microbiological organism; a nonionic surfactant; a chelant; a buffer; an antifoamer; and water.

[0066] Aspect 2 provides the biocidal composition of Aspect 1 , wherein the at least two cationic surfactants comprises a mixture of benzalkonium chloride and didecyldimethylammonium chloride.

[0067] Aspect 3 provides the biocidal composition of Aspect 2, wherein the benzalkonium chloride is in a range of from about 12 wt% to about 22 wt% of the dry weight of the biocidal composition.SUBSTITUTE SHEET (RULE 26)

[0066] Aspect 2 provides the biocidal composition of Aspect 1, wherein the at least two cationic surfactants comprises a mixture of benzalkonium chloride and didecyldimethylammonium chloride.

[0067] Aspect 3 provides the biocidal composition of Aspect 2, wherein the benzalkonium chloride is in a range of from about 12 wt% to about 22 wt% of the dry weight of the biocidal composition.

[0068] Aspect 4 provides the biocidal composition of any one of Aspects 2 or 3, wherein the benzalkonium chloride is in a range of from about 15 wt% to about 20 wt% of the biocidal composition.

[0069] Aspect 5 provides the biocidal composition of any one of Aspects 2-4, wherein the didecyldimethylammonium chloride is in a range of from about 0.5 wt% to about 7 wt% of the biocidal composition.

[0070] Aspect 6 provides the biocidal composition of any one of Aspects 2-5, wherein the didecyldimethylammonium chloride is in a range of from about 2.5 wt% to about 3.5 wt% of the biocidal composition.

[0071] Aspect 7 provides the biocidal composition of any one of Aspects 1-6, wherein the at least two cationic surfactants together range from about 15 wt% to about 60 wt% of the biocidal composition.

[0072] Aspect 8 provides the biocidal composition of any one of Aspects 1-7, wherein the at least two cationic surfactants together range from about 20 wt% to about 45 wt% of the biocidal composition.

[0073] Aspect 9 provides the biocidal composition of any one of Aspects 1-8, wherein the non-ionic surfactant comprises an alkyl polyglycoside.

[0074] Aspect 10 provides the biocidal composition of any one of Aspects 1 -9, wherein the non-ionic surfactant is in a range of from about 4 wt% to about 16 wt% of the biocidal composition.

[0075] Aspect 11 provides the biocidal composition of any one of Aspects 1-10, wherein the non-ionic surfactant is in a range of from about 5 wt% to about 10 wt% of the biocidal composition.

[0076] Aspect 12 provides the biocidal composition of any one of Aspects 1-11, wherein the component active against a microbiological organism is in a range of from about 0.1 wt% to about 0.8 wt% of the biocidal composition.

[0077] Aspect 13 provides the biocidal composition of any one of Aspects 1-12, wherein the component active against a microbiological organism is in a range of from about 0.2 wt% to about 0.4 wt% of the biocidal composition.

[0078] Aspect 14 provides the biocidal composition of any one of Aspects 1-13, wherein the component active against a microbiological organism comprises chlorohexidine digluconate.

[0079] Aspect 15 provides the biocidal composition of any one of Aspects 1-14, wherein the chelant is in a range of from about 0.05 wt% to about 1.5 wt% of the biocidal composition.

[0080] Aspect 16 provides the biocidal composition of any one of Aspects 1-15, wherein the chelant is in a range of from about 0.2 wt% to about 0.7 wt% of the biocidal composition.

[0081] Aspect 17 provides the biocidal composition of any one of Aspects 1-16, further comprising a stabilizer.

[0082] Aspect 18 provides the biocidal composition of Aspect 17, wherein the stabilizer comprises 2,6-diphenyl-4,8diazoadamantan-l-one or derivative thereof .

[0083] Aspect 19 provides the biocidal composition of any one of Aspects 1-18, wherein the stabilizer is in a range of from about 0.005 wt% to about 0.05 wt% of the biocidal composition.

[0084] Aspect 20 provides the biocidal composition of any one of Aspects 1-19, wherein the stabilizer is in a range of from about 0.008 wt% to about 0.02 wt% of the biocidal composition.

[0085] Aspect 21 provides the biocidal composition of any one of Aspects 1-20, wherein the buffer is in a range of from about 1 wt% to about 5 wt% of the biocidal composition.

[0086] Aspect 22 provides the biocidal composition of any one of Aspects 1-21, wherein the buffer is in a range of from about 2 wt% to about 4 wt% of the biocidal composition.

[0087] Aspect 23 provides the biocidal composition of any one of Aspects 1 -22, wherein the buffer comprises a mixture of Na2HPO4 and KH2PO4.

[0088] Aspect 24 provides the biocidal composition of Aspect 23, wherein the Na2HPO4 is in a range of from about 1 wt% to about 3 wt% of the biocidal composition.

[0089] Aspect 25 provides the biocidal composition of any one of Aspects 23 or 24, wherein the Na2HPO4 is in a range of from about 1.5 wt% to about 2.5 wt% of the biocidal composition.

[0090] Aspect 26 provides the biocidal composition of any one of Aspects 23-25, wherein the KH2PO4 is in a range of from about 0.5 wt% to about 1.5 wt% of the biocidal composition.

[0091] Aspect 27 provides the biocidal composition of any one of Aspects 23-26, wherein the KH2PO4 is in a range of from about 0.7 wt% to about 1.3 wt% of the biocidal composition.

[0092] Aspect 28 provides the biocidal composition of any one of Aspects 1-27, wherein the antifoamer comprises an active silicone composition.

[0093] Aspect 29 provides the biocidal composition of any one of Aspects 1-28, wherein the antifoamer is in a range of from about 0.05 wt% to about 0.30 wt% of the biocidal composition.

[0094] Aspect 30 provides the biocidal composition of any one of Aspects 1-29, wherein the antifoamer is in a range of from about 0.10 wt% to about 0.20 wt% of the biocidal composition.

[0095] Aspect 31 provides the biocidal composition of any one of Aspects 1-30, further comprising an enzymes component.

[0096] Aspect 32 provides the biocidal composition of Aspect 31, wherein one of enzyme of the enzyme component is a protease.

[0097] Aspect 33 provides the biocidal composition of any one of Aspects 31 or 32, wherein the enzyme component is in a range of from about 1 wt% to about 5 wt% of the biocidal composition.

[0098] Aspect 34 provides the biocidal composition of any one of Aspects 31-33, wherein the enzyme component is in a range of from about 1 wt% to about 2.5 wt% of the biocidal composition.

[0099] Aspect 40 provides the biocidal composition of any one of Aspects 1-39, wherein the biocidal composition is a concentrated composition.

[0100] Aspect 41 provides the biocidal composition of Aspect 40, wherein the concentrated composition is present in a diluted formula in a range of from about 0.1 wt% to about 10 wt%.

[0101] Aspect 42 provides the biocidal composition of any one of Aspect 40 or 41 , wherein the concentrated composition is present in a diluted formula in a range of from about 0.2 wt% to about 5 wt%.

[0102] Aspect 43 provides a biocidal composition comprising: a mixture of benzalkonium chloride and di decyldimethylammonium chloride; a component active against a microbiological organism; an alkyl polyglycoside; a chlorohexidine digluconate; a buffer comprising a mixture of Na2HPO4 and KH2PO4; an antifoamer comprising an active silicone composition; a chelant; and water.

[0103] Aspect 44 provides the biocidal composition of Aspect 43, wherein the benzalkonium chloride is in a range of from about 12 wt% to about 22 wt% of the dry weight of the biocidal composition.

[0104] Aspect 45 provides the biocidal composition of any one of Aspects 43 or 44, wherein the benzalkonium chloride is in a range of from about 15 wt% to about 20 wt% of the biocidal composition.

[0105] Aspect 46 provides the biocidal composition of any one of Aspects 43-45, wherein the di decyldimethylammonium chloride is in a range of from about 0.5 wt% to about 7 wt% of the biocidal composition.

[0106] Aspect 47 provides the biocidal composition of any one of Aspects 43-46, wherein the di decyldimethylammonium chloride is in a range of from about 2.5 wt% to about 3.5 wt% of the biocidal composition.

[0107] Aspect 48 provides the biocidal composition of any one of Aspects 43-47, wherein the alkyl polyglycoside is in a range of from about 4 wt% to about 16 wt% of the biocidal composition.

[0108] 49 provides the biocidal composition of any one of Aspects 43-48, wherein the alkyl polyglycoside is in a range of from about 5 wt% to about 10 wt% of the biocidal composition.

[0109] Aspect 50 provides the biocidal composition of any one of Aspects 43-49, wherein the chlorohexidine digluconate is in a range of from about 0.1 wt% to about 0.8 wt% of the biocidal composition.

[0110] Aspect 51 provides the biocidal composition of any one of Aspects 43-50, wherein the chlorohexidine digluconate is in a range of from about 0.2 wt% to about 0.4 wt% of the biocidal composition.

[0111] Aspect 52 provides the biocidal composition of any one of Aspects 43-51, wherein the buffer is in a range of from about 1 wt% to about 5 wt% of the biocidal composition.

[0112] Aspect 53 provides the biocidal composition of any one of Aspects 43-52, wherein the buffer is in a range of from about 1 wt% to about 3 wt% of the biocidal composition.

[0113] Aspect 54 provides the biocidal composition of Aspect 53, wherein the Na2HPO4 is in a range of from about 1 wt% to about 3 wt% of the biocidal composition.

[0114] Aspect 55 provides the biocidal composition of any one of Aspects 53 or 54, wherein the Na2HPO4 is in a range of from about 1.5 wt% to about 2.5 wt% of the biocidal composition.

[0115] Aspect 56 provides the biocidal composition of any one of Aspects 53-55, wherein the KH2PO4 is in a range of from about 0.5 wt% to about 1.5 wt% of the biocidal composition.

[0116] Aspect 57 provides the biocidal composition of any one of Aspects 53-56, wherein the KH2PO4 is in a range of from about 0.7 wt% to about 1.3 wt% of the biocidal composition.

[0117] Aspect 58 provides the biocidal composition of any one of Aspects 43-57, wherein the antifoamer is in a range of from about 0.05 wt% to about 0.30 wt% of the biocidal composition.

[0118] Aspect 59 provides the biocidal composition of any one of Aspects 43-58, wherein the antifoamer is in a range of from about 0.10 wt% to about 0.20 wt% of the biocidal composition.

[0119] Aspect 60 provides the biocidal composition of any one of Aspects 43-59, further comprising an enzyme component.

[0120] Aspect 61 provides the biocidal composition of Aspect 60, wherein the enzyme component is a protease.

[0121] Aspect 62 provides the biocidal composition of any one of Aspects 60 or 61, wherein the enzyme component is in a range of from about 1 wt% to about 5 wt% of the biocidal composition.

[0122] Aspect 63 provides the biocidal composition of any one of Aspects 60-62, wherein the enzyme component is in a range of from about 1 wt% to about 3 wt% of the biocidal composition.

[0123] Aspect 64 provides the biocidal composition of any one of Aspects 43-63, wherein the biocidal composition is effective or active against bacteria, fungus, yeast, a virus, or a mixture thereof.

[0124] Aspect 65 provides the biocidal composition of Aspect 64, wherein the biocidal composition is effective or active against P. aeruginosa, E. hirae, S. aureus, C. albicans, A. brasiliensis, Adenovirus, modified vaccinia Ankara, or a mixture thereof.

[0125] Aspect 66 provides the biocidal composition of any one of Aspects 64 or 65, wherein a LogR of the bacteria, fungus, yeast, a virus, or a mixture thereof is greater than or equal to 3.

[0126] Aspect 67 provides the biocidal composition of any one of Aspects 64-66, wherein a LogR of the bacteria, yeast, or a mixture thereof is greater than or equal to 4.

[0127] Aspect 68 provides the biocidal composition of any one of Aspects 64-67, wherein the biocidal composition is effective or active against bacteria and yeast.

[0128] Aspect 69 provides the biocidal composition of any one of Aspects 43-68, wherein the biocidal composition is a concentrated composition.

[0129] Aspect 70 provides the biocidal composition of Aspect 69, wherein the concentrated composition is present in a diluted formula in a range of from about 1 wt% to about 10 wt%.

[0130] Aspect 71 provides the biocidal composition of any one of Aspect 69 or 70, wherein the concentrated composition is present in a diluted formula in a range of from about 0.2 wt% to about 5 wt%.

[0131] Aspect 72 provides a method of using the biocidal composition of any one of Aspects 1-71, the method comprising applying the biocidal composition to at least a portion of a surface.

[0132] Aspect 73 provides the method of Aspect 72, wherein the surface is a surface of a medical device, an industrial surface, or a residential surface.

[0133] Aspect 74 provides the method of Aspect 73, wherein the medical device is an invasive or non-invasive medical device.

Claims

CLAIMSWhat is claimed is:

1. A biocidal composition comprising: a mixture of at least two cationic surfactants; a component active against a microbiological organism; a nonionic surfactant; a chelant; a buffer; an antifoamer; and water.

2. The biocidal composition of claim 1 , wherein the at least two cationic surfactants comprises a mixture of benzalkonium chloride and didecyldimethylammonium chloride.

3. The biocidal composition of claim 2, wherein the benzalkonium chloride is in a range of from about 12 wt% to about 22 wt% of the dry weight of the biocidal composition.

4. The biocidal composition of any one of claims 2 or 3, wherein the benzalkonium chloride is in a range of from about 15 wt% to about 20 wt% of the biocidal composition.

5. The biocidal composition of any one of claims 2-4, wherein the di decyldimethylammonium chloride is in a range of from about 0.5 wt% to about 7 wt% of the biocidal composition.

6. The biocidal composition of any one of claims 2-5, wherein the di decyldimethylammonium chloride is in a range of from about 2.5 wt% to about 3.5 wt% of the biocidal composition.

7. The biocidal composition of any one of claims 1-6, wherein the at least two cationic surfactants together range from about 15 wt% to about 60 wt% of the biocidal composition.

8. The biocidal composition of any one of claims 1-7, wherein the at least two cationic surfactants together range from about 20 wt% to about 45 wt% of the biocidal composition.

9. The biocidal composition of any one of claims 1-8, wherein the non-ionic surfactant comprises an alkyl polyglycoside.

10. The biocidal composition of any one of claims 1-9, wherein the non-ionic surfactant is in a range of from about 4 wt% to about 16 wt% of the biocidal composition.

11. The biocidal composition of any one of claims 1-10, wherein the non-ionic surfactant is in a range of from about 5 wt% to about 10 wt% of the biocidal composition.

12. The biocidal composition of any one of claims 1-11, wherein the component active against a microbiological organism is in a range of from about 0.1 wt% to about 0.8 wt% of the biocidal composition.

13. The biocidal composition of any one of claims 1-12, wherein the component active against a microbiological organism is in a range of from about 0.2 wt% to about 0.4 wt% of the biocidal composition.

14. The biocidal composition of any one of claims 1-13, wherein the component active against a microbiological organism comprises chlorohexidine digluconate.

15. The biocidal composition of any one of claims 1-14, wherein the chelant is in a range of from about 0.05 wt% to about 1.5 wt% of the biocidal composition.

16. The biocidal composition of any one of claims 1-15, wherein the chelant is in a range of from about 0.2 wt% to about 0.7 wt% of the biocidal composition.

17. The biocidal composition of any one of claims 1-16, further comprising a stabilizer.

18. The biocidal composition of claim 17, wherein the stabilizer comprises 2,6-diphenyl- 4,8diazoadamantan-l-one or derivative thereof.

19. The biocidal composition of any one of claims 1-18, wherein the stabilizer is in a range of from about 0.005 wt% to about 0.05 wt% of the biocidal composition.

20. The biocidal composition of any one of claims 1-19, wherein the stabilizer is in a range of from about 0.008 wt% to about 0.02 wt% of the biocidal composition.

21. The biocidal composition of any one of claims 1-20, wherein the buffer is in a range of from about 1 wt% to about 5 wt% of the biocidal composition.

22. The biocidal composition of any one of claims 1-21, wherein the buffer is in a range of from about 2 wt% to about 4 wt% of the biocidal composition.

23. The biocidal composition of any one of claims 1-22, wherein the buffer comprises a mixture of Na2HPO4 and KH2PO4.

24. The biocidal composition of claim 23, wherein the Na2HPO4 is in a range of from about 1 wt% to about 3 wt% of the biocidal composition.

25. The biocidal composition of any one of claims 23 or 24, wherein the Na2HPO4 is in a range of from about 1.5 wt% to about 2.5 wt% of the biocidal composition.

26. The biocidal composition of any one of claims 23-25, wherein the KH2PO4 is in a range of from about 0.5 wt% to about 1.5 wt% of the biocidal composition.

27. The biocidal composition of any one of claims 23-26, wherein the KH2PO4 is in a range of from about 0.7 wt% to about 1.3 wt% of the biocidal composition.

28. The biocidal composition of any one of claims 1-27, wherein the antifoamer comprises an active silicone composition.

29. The biocidal composition of any one of claims 1-28, wherein the antifoamer is in a range of from about 0.05 wt% to about 0.30 wt% of the biocidal composition.

30. The biocidal composition of any one of claims 1-29, wherein the antifoamer is in a range of from about 0.10 wt% to about 0.20 wt% of the biocidal composition.

31. The biocidal composition of any one of claims 1-30, further comprising an enzymes component.

32. The biocidal composition of claim 31, wherein one of enzyme of the enzyme component is a protease.

33. The biocidal composition of any one of claims 31 or 32, wherein the enzyme component is in a range of from about 1 wt% to about 5 wt% of the biocidal composition.

34. The biocidal composition of any one of claims 31-33, wherein the enzyme component is in a range of from about 1 wt% to about 3 wt% of the biocidal composition.

40. The biocidal composition of any one of claims 1-39, wherein the biocidal composition is a concentrated composition.

41. The biocidal composition of claim 40, wherein the concentrated composition is present in a diluted formula in a range of from about 0.1 wt% to about 10 wt%.

42. The biocidal composition of any one of claim 40 or 41, wherein the concentrated composition is present in a diluted formula in a range of from about 0.2 wt% to about 5 wt%.

43. A biocidal composition comprising: a mixture of benzalkonium chloride and di decyldimethylammonium chloride; a component active against a microbiological organism; an alkyl polyglycoside; a chlorohexidine digluconate; a buffer comprising a mixture of Na2HPO4 and KH2PO4; an antifoamer comprising an active silicone composition; a chelant; and water.

44. The biocidal composition of claim 43, wherein the benzalkonium chloride is in a range of from about 12 wt% to about 22 wt% of the dry weight of the biocidal composition.

45. The biocidal composition of any one of claims 43 or 44, wherein the benzalkonium chloride is in a range of from about 15 wt% to about 20 wt% of the biocidal composition.

46. The biocidal composition of any one of claims 43-45, wherein the di decyldimethylammonium chloride is in a range of from about 0.5 wt% to about 7 wt% of the biocidal composition.

47. The biocidal composition of any one of claims 43-46, wherein the di decyldimethylammonium chloride is in a range of from about 2.5 wt% to about 3.5 wt% of the biocidal composition.

48. The biocidal composition of any one of claims 43-47, wherein the alkyl polyglycoside is in a range of from about 4 wt% to about 16 wt% of the biocidal composition.

49. The biocidal composition of any one of claims 43-48, wherein the alkyl polyglycoside is in a range of from about 5 wt% to about 10 wt% of the biocidal composition.

50. The biocidal composition of any one of claims 43-49, wherein the chlorohexidine digluconate is in a range of from about 1 wt% to about 0.8 wt% of the biocidal composition.

51. The biocidal composition of any one of claims 43-50, wherein the chlorohexidine digluconate is in a range of from about 0.2 wt% to about 0.4 wt% of the biocidal composition.

52. The biocidal composition of any one of claims 43-51, wherein the buffer is in a range of from about 1 wt% to about 5 wt% of the biocidal composition.

53. The biocidal composition of any one of claims 43-52, wherein the buffer is in a range of from about 1 wt% to about 3 wt% of the biocidal composition.

54. The biocidal composition of claim 53, wherein the Na2HPO4 is in a range of from about 1 wt% to about 3 wt% of the biocidal composition.

55. The biocidal composition of any one of claims 53 or 54, wherein the Na2HPO4 is in a range of from about 1.5 wt% to about 2.5 wt% of the biocidal composition.

56. The biocidal composition of any one of claims 53-55, wherein the KH2PO4 is in a range of from about 0.5 wt% to about 1.5 wt% of the biocidal composition.

57. The biocidal composition of any one of claims 53-56, wherein the KH2PO4 is in a range of from about 0.7 wt% to about 1.3 wt% of the biocidal composition.

58. The biocidal composition of any one of claims 43-57, wherein the antifoamer is in a range of from about 0.05 wt% to about 0.30 wt% of the biocidal composition.

59. The biocidal composition of any one of claims 43-58, wherein the antifoamer is in a range of from about 0.10 wt% to about 0.20 wt% of the biocidal composition.

60. The biocidal composition of any one of claims 43-59, further comprising an enzyme component.

61. The biocidal composition of claim 60, wherein the enzyme component is a protease.

62. The biocidal composition of any one of claims 60 or 61, wherein the enzyme component is in a range of from about 1 wt% to about 5 wt% of the biocidal composition.

63. The biocidal composition of any one of claims 60-62, wherein the enzyme component is in a range of from about 1 wt% to about 3 wt% of the biocidal composition.

64. The biocidal composition of any one of claims 43-63, wherein the biocidal composition is effective or active against bacteria, fungus, yeast, a virus, or a mixture thereof.

65. The biocidal composition of claim 64, wherein the biocidal composition is effective or active against P. aeruginosa, E. hirae, S. aureus, C. albicans, A. brasiliensis, Adenovirus, modified vaccinia Ankara, or a mixture thereof.

66. The biocidal composition of any one of claims 64 or 65, wherein a LogR of the bacteria, fungus, yeast, a virus, or a mixture thereof is greater than or equal to 3.

67. The biocidal composition of any one of claims 64-66, wherein a LogR of the bacteria, yeast, or a mixture thereof is greater than or equal to 4.

68. The biocidal composition of any one of claims 64-67, wherein the biocidal composition is effective or active against bacteria and yeast.

69. The biocidal composition of any one of claims 43-68, wherein the biocidal composition is a concentrated composition.

70. The biocidal composition of claim 69, wherein the concentrated composition is present in a diluted formula in a range of from about 1 wt% to about 10 wt%.

71. The biocidal composition of any one of claim 69 or 70, wherein the concentrated composition is present in a diluted formula in a range of from about 2 wt% to about 5 wt%.

72. A method of using the biocidal composition of any one of claims 1-71, the method comprising applying the biocidal composition to at least a portion of a surface.

73. The method of claim 72, wherein the surface is a surface of a medical device, an industrial surface, or a residential surface.

74. The method of claim 73, wherein the medical device is an invasive or non-invasive medical device.24[0068] Aspect 4 provides the biocidal composition of any one of Aspects 2 or 3, wherein the benzalkonium chloride is in a range of from about 15 wt% to about 20 wt% of the biocidal composition.[0069] Aspect 5 provides the biocidal composition of any one of Aspects 2-4, wherein the didecyldimethylammonium chloride is in a range of from about 0.5 wt% to about 7 wt% of the biocidal composition.[0070] Aspect 6 provides the biocidal composition of any one of Aspects 2-5, wherein the didecyldimethylammonium chloride is in a range of from about 2.5 wt% to about 3.5 wt% of the biocidal composition.[0071] Aspect 7 provides the biocidal composition of any one of Aspects 1-6, wherein the at least two cationic surfactants together range from about 15 wt% to about 60 wt% of the biocidal composition.[0072] Aspect 8 provides the biocidal composition of any one of Aspects 1-7, wherein the at least two cationic surfactants together range from about 20 wt% to about 45 wt% of the biocidal composition.[0073] Aspect 9 provides the biocidal composition of any one of Aspects 1-8, wherein the non-ionic surfactant comprises an alkyl polyglycoside.[0074] Aspect 10 provides the biocidal composition of any one of Aspects 1-9, wherein the non-ionic surfactant is in a range of from about 4 wt% to about 16 wt% of the biocidal composition.[0075] Aspect 11 provides the biocidal composition of any one of Aspects 1-10, wherein the non-ionic surfactant is in a range of from about 5 wt% to about 10 wt% of the biocidal composition.[0076] Aspect 12 provides the biocidal composition of any one of Aspects 1-11, wherein the component active against a microbiological organism is in a range of from about 0.1 wt% to about 0.8 wt% of the biocidal composition.[0077] Aspect 13 provides the biocidal composition of any one of Aspects 1-12, wherein the component active against a microbiological organism is in a range of from about 0.2 wt% to about 0.4 wt% of the biocidal composition.SUBSTITUTE SHEET (RULE 26)25[0078] Aspect 14 provides the biocidal composition of any one of Aspects 1-13, wherein the component active against a microbiological organism comprises chlorohexidine digluconate. [0079] Aspect 15 provides the biocidal composition of any one of Aspects 1-14, wherein the chelant is in a range of from about 0.05 wt% to about 1.5 wt% of the biocidal composition.[0080] Aspect 16 provides the biocidal composition of any one of Aspects 1-15, wherein the chelant is in a range of from about 0.2 wt% to about 0.7 wt% of the biocidal composition.[0081] Aspect 17 provides the biocidal composition of any one of Aspects 1-16, further comprising a stabilizer.[0082] Aspect 18 provides the biocidal composition of Aspect 17, wherein the stabilizer comprises 2,6-diphenyl-4,8diazoadamantan-l-one or derivative thereof.[0083] Aspect 19 provides the biocidal composition of any one of Aspects 1-18, wherein the stabilizer is in a range of from about 0.005 wt% to about 0.05 wt% of the biocidal composition.[0084] Aspect 20 provides the biocidal composition of any one of Aspects 1-19, wherein the stabilizer is in a range of from about 0.008 wt% to about 0.02 wt% of the biocidal composition.[0085] Aspect 21 provides the biocidal composition of any one of Aspects 1-20, wherein the buffer is in a range of from about 1 wt% to about 5 wt% of the biocidal composition.[0086] Aspect 22 provides the biocidal composition of any one of Aspects 1-21, wherein the buffer is in a range of from about 2 wt% to about 4 wt% of the biocidal composition.[0087] Aspect 23 provides the biocidal composition of any one of Aspects 1-22, wherein the buffer comprises a mixture of NaiHPC and KH2PO4.[0088] Aspect 24 provides the biocidal composition of Aspect 23, wherein the NaiHPCh is in a range of from about 1 wt% to about 3 wt% of the biocidal composition.[0089] Aspect 25 provides the biocidal composition of any one of Aspects 23 or 24, wherein the NaiHPCh is in a range of from about 1.5 wt% to about 2.5 wt% of the biocidal composition.[0090] Aspect 26 provides the biocidal composition of any one of Aspects 23-25, wherein the KH2PO4 is in a range of from about 0.5 wt% to about 1.5 wt% of the biocidal composition.SUBSTITUTE SHEET (RULE 26)26[0091] Aspect 27 provides the biocidal composition of any one of Aspects 23-26, wherein the KH2PO4 is in a range of from about 0.7 wt% to about 1.3 wt% of the biocidal composition.[0092] Aspect 28 provides the biocidal composition of any one of Aspects 1-27, wherein the antifoamer comprises an active silicone composition.[0093] Aspect 29 provides the biocidal composition of any one of Aspects 1-28, wherein the antifoamer is in a range of from about 0.05 wt% to about 0.30 wt% of the biocidal composition.[0094] Aspect 30 provides the biocidal composition of any one of Aspects 1-29, wherein the antifoamer is in a range of from about 0.10 wt% to about 0.20 wt% of the biocidal composition.[0095] Aspect 31 provides the biocidal composition of any one of Aspects 1-30, further comprising an enzymes component.[0096] Aspect 32 provides the biocidal composition of Aspect 31 , wherein one of enzyme of the enzyme component is a protease.[0097] Aspect 33 provides the biocidal composition of any one of Aspects 31 or 32, wherein the enzyme component is in a range of from about 1 wt% to about 5 wt% of the biocidal composition.[0098] Aspect 34 provides the biocidal composition of any one of Aspects 31-33, wherein the enzyme component is in a range of from about 1 wt% to about 2.5 wt% of the biocidal composition.[0099] Aspect 40 provides the biocidal composition of any one of Aspects 1-39, wherein the biocidal composition is a concentrated composition.[0100] Aspect 41 provides the biocidal composition of Aspect 40, wherein the concentrated composition is present in a diluted formula in a range of from about 0.1 wt% to about 10 wt%.[0101] Aspect 42 provides the biocidal composition of any one of Aspect 40 or 41 , wherein the concentrated composition is present in a diluted formula in a range of from about 0.2 wt% to about 5 wt%.[0102] Aspect 43 provides a biocidal composition comprising:SUBSTITUTE SHEET (RULE 26)27 a mixture of benzalkonium chloride and didecyldimethylammonium chloride; a component active against a microbiological organism; an alkyl polyglycoside; a chlorohexidine digluconate; a buffer comprising a mixture of NaiHPC and KH2PO4; an antifoamer comprising an active silicone composition; a chelant; and water.[0103] Aspect 44 provides the biocidal composition of Aspect 43, wherein the benzalkonium chloride is in a range of from about 12 wt% to about 22 wt% of the dry weight of the biocidal composition.[0104] Aspect 45 provides the biocidal composition of any one of Aspects 43 or 44, wherein the benzalkonium chloride is in a range of from about 15 wt% to about 20 wt% of the biocidal composition.[0105] Aspect 46 provides the biocidal composition of any one of Aspects 43-45, wherein the di decyldimethylammonium chloride is in a range of from about 0.5 wt% to about 7 wt% of the biocidal composition.[0106] Aspect 47 provides the biocidal composition of any one of Aspects 43-46, wherein the di decyldimethylammonium chloride is in a range of from about 2.5 wt% to about 3.5 wt% of the biocidal composition.[0107] Aspect 48 provides the biocidal composition of any one of Aspects 43-47, wherein the alkyl polyglycoside is in a range of from about 4 wt% to about 16 wt% of the biocidal composition.[0108] 49 provides the biocidal composition of any one of Aspects 43-48, wherein the alkyl polyglycoside is in a range of from about 5 wt% to about 10 wt% of the biocidal composition.[0109] Aspect 50 provides the biocidal composition of any one of Aspects 43-49, wherein the chlorohexidine digluconate is in a range of from about 0.1 wt% to about 0.8 wt% of the biocidal composition.SUBSTITUTE SHEET (RULE 26)28[0110] Aspect 51 provides the biocidal composition of any one of Aspects 43-50, wherein the chlorohexidine digluconate is in a range of from about 0.2 wt% to about 0.4 wt% of the biocidal composition.[0111] Aspect 52 provides the biocidal composition of any one of Aspects 43-51, wherein the buffer is in a range of from about 1 wt% to about 5 wt% of the biocidal composition. [0112] Aspect 53 provides the biocidal composition of any one of Aspects 43-52, wherein the buffer is in a range of from about 1 wt% to about 3 wt% of the biocidal composition. [0113] Aspect 54 provides the biocidal composition of Aspect 53, wherein the NaiHPCh is in a range of from about 1 wt% to about 3 wt% of the biocidal composition.[0114] Aspect 55 provides the biocidal composition of any one of Aspects 53 or 54, wherein the NaiHPCh is in a range of from about 1.5 wt% to about 2.5 wt% of the biocidal composition.[0115] Aspect 56 provides the biocidal composition of any one of Aspects 53-55, wherein the KH2PO4 is in a range of from about 0.5 wt% to about 1.5 wt% of the biocidal composition.[0116] Aspect 57 provides the biocidal composition of any one of Aspects 53-56, wherein the KH2PO4 is in a range of from about 0.7 wt% to about 1.3 wt% of the biocidal composition.[0117] Aspect 58 provides the biocidal composition of any one of Aspects 43-57, wherein the antifoamer is in a range of from about 0.05 wt% to about 0.30 wt% of the biocidal composition.[0118] Aspect 59 provides the biocidal composition of any one of Aspects 43-58, wherein the antifoamer is in a range of from about 0.10 wt% to about 0.20 wt% of the biocidal composition.[0119] Aspect 60 provides the biocidal composition of any one of Aspects 43-59, further comprising an enzyme component.[0120] Aspect 61 provides the biocidal composition of Aspect 60, wherein the enzyme component is a protease.SUBSTITUTE SHEET (RULE 26)29[0121] Aspect 62 provides the biocidal composition of any one of Aspects 60 or 61, wherein the enzyme component is in a range of from about 1 wt% to about 5 wt% of the biocidal composition.[0122] Aspect 63 provides the biocidal composition of any one of Aspects 60-62, wherein the enzyme component is in a range of from about 1 wt% to about 3 wt% of the biocidal composition.[0123] Aspect 64 provides the biocidal composition of any one of Aspects 43-63, wherein the biocidal composition is effective or active against bacteria, fungus, yeast, a virus, or a mixture thereof.[0124] Aspect 65 provides the biocidal composition of Aspect 64, wherein the biocidal composition is effective or active against P. aeruginosa, E. hirae, S. aureus, C. albicans, A. brasiliensis, Adenovirus, modified vaccinia Ankara, or a mixture thereof.[0125] Aspect 66 provides the biocidal composition of any one of Aspects 64 or 65, wherein a LogR of the bacteria, fungus, yeast, a virus, or a mixture thereof is greater than or equal to 3.[0126] Aspect 67 provides the biocidal composition of any one of Aspects 64-66, wherein a LogR of the bacteria, yeast, or a mixture thereof is greater than or equal to 4.[0127] Aspect 68 provides the biocidal composition of any one of Aspects 64-67, wherein the biocidal composition is effective or active against bacteria and yeast.[0128] Aspect 69 provides the biocidal composition of any one of Aspects 43-68, wherein the biocidal composition is a concentrated composition.[0129] Aspect 70 provides the biocidal composition of Aspect 69, wherein the concentrated composition is present in a diluted formula in a range of from about 1 wt% to about 10 wt%.[0130] Aspect 71 provides the biocidal composition of any one of Aspect 69 or 70, wherein the concentrated composition is present in a diluted formula in a range of from about 0.2 wt% to about 5 wt%.[0131] Aspect 72 provides a method of using the biocidal composition of any one of Aspects 1-71, the method comprising applying the biocidal composition to at least a portion of a surface.SUBSTITUTE SHEET (RULE 26)30[0132] Aspect 73 provides the method of Aspect 72, wherein the surface is a surface of a medical device, an industrial surface, or a residential surface.[0133] Aspect 74 provides the method of Aspect 73, wherein the medical device is an invasive or non-invasive medical device.SUBSTITUTE SHEET (RULE 26)

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