Method for determining the concentration of mineral particles in formulations, and kit

Abstract

The invention relates to a method for determining the concentration of mineral particles in a formulation and in particular in a toothpaste.

Description

[0001] Methods for determining the mineral particle content in formulations

[0002] The invention relates to a method for determining the content of mineral particles in a formulation and in particular in a toothpaste.

[0003] Mineral particles are inorganic particles that originate from natural or synthetic sources and can exist in various sizes and shapes.

[0004] Mineral particles are used in numerous products, including cosmetics, pharmaceuticals, food, paints, and varnishes. These particles fulfill various functions, such as contributing to the product's function, color, texture, cleaning, protection, stabilization, and shine. They can also serve as fillers and thickeners. Typically, mineral particles are very small and light gray to white, making them difficult to reliably detect with a light microscope due to the low contrast.

[0005] Titanium dioxide is a commonly used mineral. It is used as a white pigment in numerous products, including sunscreens, toothpastes, and paints. Calcium phosphates are also frequently used, for example, in the form of hydroxyapatite in oral care products for tooth remineralization and caries protection (M. Pawinska, E. Paszynska, H. Limeback, BT Amaechi, H.-O. Fabritius, B. Ganss, K. O'Hagan-Wong, E. Schulze zur Wiesche, F. Meyer, J. Enax, Hydroxyapatite as an active ingredient in oral care: an international symposium report, Bioinspired Biomim. Nanobiomaterials 13 (2024) 1-14).

[0006] Besides selecting a suitable mineral type for the specific application, the content of mineral particles is of particular importance for the function of the respective product type. For example, it is known that UV protection in sunscreens increases with a higher titanium dioxide content. Another example is that a higher concentration of silica abrasives in toothpastes correlates positively with the cleaning performance for plaque removal. It is also known that the higher the concentration of calcium phosphates such as hydroxyapatite in toothpastes, the greater their effectiveness. Various methods can be used to determine the content of mineral particles in formulations. These include atomic absorption spectroscopy (AAS), X-ray fluorescence spectroscopy (XRF), inductively coupled plasma atomic emission spectroscopy (ICP-AES), UV-Vis spectroscopy, and infrared (IR) spectroscopy.

[0007] However, all these methods have the disadvantage of being time-consuming, technically demanding, and expensive, as well as requiring complex sample preparation. Furthermore, sample preparation, measurement execution, and data analysis require in-depth expert knowledge.

[0008] The object of the invention is therefore to create a new method for the simple and rapid determination of the content of mineral particles in different formulations, which avoids the disadvantages of the prior art described above.

[0009] A first aspect of the invention relates to a method for determining the content of mineral particles in a formulation with a viscosity in the range of 1 mPas to 200,000 mPas, wherein the method comprises the steps

[0010] (a) Applying the formulation to a test matrix, preferably a test strip, wherein the surface of the test matrix, preferably of the test strip, on which the formulation is applied has a defined roughness with a mean arithmetic height in the range of 0.1 to 500 pm,

[0011] (b) Determination and evaluation of the mineral content.

[0012] The method according to the invention makes it possible to determine the total mineral content in different formulations. Both the measurement and the evaluation can be carried out quickly and reliably, even by non-experts, including end consumers.

[0013] One area of ​​application is quality control, e.g. to ensure the desired proportion of mineral particles in formulations.

[0014] Another area of ​​application is the determination of the mineral particle content in unknown formulations. In particular, the invention also provides a method which makes it possible to easily and quickly compare the total mineral content of at least two formulations relative to each other by parallel application to at least one test matrix, preferably at least one test strip, or especially by using at least two test strips.

[0015] The method according to the invention is suitable for determining the content of mineral particles in a formulation with a viscosity in the range of 1 mPas to 200,000 mPas.

[0016] In embodiments according to the invention relating to pastes and in particular toothpastes, the viscosity is preferably in the range of 1 - 150,000 mPas, more preferably between 5,000 - 120,000 mPas and particularly preferably in the range of 10,000 - 90,000 mPas (in each case determined 0-4 days after manufacture).

[0017] In embodiments according to the invention relating to solutions and in particular mouthwashes, the viscosity is preferably in the range of 1 - 1,000 mPas, more preferably between 1 - 500 mPas and particularly preferably in the range of 10 - 150 mPas (in each case determined 0-4 days after manufacture).

[0018] All viscosity data are based on a measurement according to DIN 53019-1 :2008-09 with a Haake RheoStressl type rheometer (ThermoFisher Scientific) at 20 °C and a gravity velocity of 10 / s (toothpaste) and 50 / s (mouthwash) in plate-plate geometry.

[0019] The surface of the test matrix, preferably the test strip, onto which the formulation is applied, has a defined roughness with a mean arithmetic height in the range of 0.1 to 500 pm, i.e., it is rough or has a grain structure. Surprisingly, it was found that the method works particularly reliably on inorganic surfaces with a mean arithmetic height of the surface between 0.1 pm and 500 pm.

[0020] The test matrix surface, preferably a test strip surface, can be applied to any substrate material. The substrate material can be rigid or flexible. Preferably, the substrate material is selected from plastics, paper, composite materials made of cellulose, such as vulcanized fiber, and / or linen and woven fabrics.

[0021] An inorganic test matrix surface, particularly a test strip surface, may be preferred. An inorganic test matrix surface, preferably a test strip surface, may be made of any suitable inorganic material and is preferably made of silicon carbide, diamond, zirconium oxide, and / or aluminum oxide. Silicon carbide is particularly preferred.

[0022] The roughness or grain size of the test matrix surface, preferably the test tire surface, can be characterized according to the system known for sandpaper. A grain size between P10 and P10000 according to FEPA (Federation Européenne des Fabricants de Produit de Abrasifs) is preferably used. A surface with a grain size between P40 and P2000 is more preferred. A surface with a grain size between P60 and P400 is particularly preferred.

[0023] In preferred embodiments, sandpaper, preferably test strips, is used as the test matrix. In particularly preferred embodiments, commercially available sandpaper can be used. The sandpaper used is preferably applied to an additional stabilizing layer.

[0024] Sandpaper is defined as a tool for removing material by using abrasive grains on a backing layer. The surface of standard sandpaper consists of a layer of abrasive grains applied to a backing layer. The grains are arranged in a specific size and distribution to achieve a uniform abrasive effect. The backing layer can be made of paper, fabric, or plastic and is usually flexible to conform to the contours of the surface being sanded. The grains can be made of various materials such as silicon carbide, diamond, zirconium oxide, or aluminum oxide, depending on the application and desired abrasive effect. The surface of the sandpaper can have varying degrees of roughness depending on the grain size and type.

[0025] Sandpaper with a grit size between P10 and P10000 according to FEPA can be used. Sandpaper with a grit size between P40 and P2000 is preferred. Sandpaper with a grit size between P60 and P400 is particularly preferred.

[0026] For the implementation of the method according to the invention, the following silicon carbide-based abrasive papers, such as P80 - P3000 (Hawerk), P120 - P5000 (BBbanda), or 120 Cw - 10000 Cw (Yuehao Abrasive Materials Co.), are suitable, for example. Silicon carbide-based abrasive paper with the grit size P80 (Hawerk) is particularly preferred. Alternatively, a printing process can be used to create a defined surface roughness. The printing process can be selected from the following methods: bubble printing, relief printing, gravure printing, screen printing, UV spot coating, thermography, digital 3D printing, frost printing, or flock printing.

[0027] Bubble printing: A method in which a rough structure is created by drying bubbles in ink or another liquid.

[0028] Relief printing: In this method, which also includes woodcuts and linocuts, texture is created by leaving areas unprinted on the printing plate. The raised areas are inked and printed onto the paper, resulting in a textured surface.

[0029] Intaglio printing: In intaglio printing, including etching and aquatint, the ink is deposited in recessed areas of the printing plate. By varying the depth and roughness of these recesses, different textures can be created in the print.

[0030] Screen printing: By adding thickening agents or other additives to the screen printing ink, a three-dimensional texture can be created. This technique is also known as relief screen printing.

[0031] UV spot varnishing: In this method, a UV-reactive varnish is applied to specific areas of the print and then cured under UV light. This creates a raised, glossy surface that produces a tactile texture.

[0032] Thermography: In thermography, a special powder is sprinkled onto the still-wet ink and then heated. The powder melts and expands, creating a raised surface.

[0033] Digital 3D printing: Modern digital printers can apply layers of ink or other materials to create a textured surface.

[0034] Frost printing: A special printing technique used to create a matte, rough, or textured surface reminiscent of frozen glass or ice crystals. Flock printing: In flock printing, short fibers are applied to the print while the ink is still wet. The fibers adhere to the ink, creating a velvety, textured surface.

[0035] The test matrix, and in particular the test strip, is preferably characterized by a mean arithmetic height of the surface, measured by laser scanning microscopy, in the range of 0.1 pm to 500 pm, and more preferably 1.0 pm to 300 pm. More preferably, the mean arithmetic height of the surface of the test matrix, and more preferably of the test strip, is in the range of 5 pm to 250 pm, more preferably 10 pm to 100 pm, and even more preferably in the range of 40 pm to 60 pm. The measurement of the mean arithmetic height of the surface of the test matrix, and more preferably of the test strip, can be performed, for example, with a Keyence VK-X 1100 laser scanning microscope.

[0036] The surface of the test matrix, preferably the test tire, is preferably a color that provides good contrast to the mineral particles to be analyzed, generally light gray to white. Dark and / or muted colors are preferred, and in particular black, blue, green, or red.

[0037] The test matrix according to the invention, preferably a test tire, can have any dimensions suitable for the respective purpose. A typical dimension is, for example, 2 x 10 cm. According to the invention, the test matrix can also be square.

[0038] The application of the formulation to the test matrix, preferably the test strip, according to step (A) is preferably carried out homogeneously.

[0039] The formulation containing the mineral particles can be applied to the test matrix, preferably the test strip, in any suitable manner. Application to the test matrix, preferably the test strip, is preferably carried out using a finger, toothbrush, spatula, putty knife, brush, roller, or sponge. Application with a toothbrush or spatula is particularly preferred.

[0040] After application, volatile components such as water or organic solvents can be removed by drying in a drying step (a'). This drying can preferably be carried out in air at room temperature or, more preferably, by heating, for example with a hairdryer or in a drying oven. According to the invention, several formulations to be analyzed can be applied side by side and thus analyzed simultaneously.

[0041] The evaluation of the test matrix, preferably the test strip, can be done visually or digitally.

[0042] A preferred evaluation method is visual, based on the coverage of the test matrix, preferably the test strip.

[0043] Surprisingly, it was found that the mineral content of the formulation after application to the test strip was less than 1 wt.% without visible coverage of the test strip, in the range of 1 to 5 wt.% with moderate coverage of the test strip, in the range of 5 to 10 wt.% with predominant coverage of the test strip, and greater than 10 wt.% with complete coverage of the test strip.

[0044] The mineral content can be evaluated after application by comparing the gray tone of the applied coating with corresponding reference values ​​or a reference scale. This evaluation can be carried out, for example, using a gray tone scale, e.g., printed on paper such as Sigel Laser paper, LP207, DIN A4, white, printed with a Konica Minolta bizhub C450i printer (Figure 1).

[0045] The mineral content of the formulation to be analyzed can also be determined visually relative to a reference formulation. According to the invention, it is also possible to determine the mineral content of at least two formulations to be analyzed relative to each other, preferably visually.

[0046] In addition to visual evaluation, a digital evaluation of grayscale values ​​can alternatively be performed using photographs. Grayscale values ​​can be determined, for example, with software products such as ImageJ (National Institutes of Health, Bethesda, USA) or Adobe Photoshop® (Adobe Inc.). Reference values ​​from the grayscale scale can be determined and used to compare individual photographs.

[0047] The method according to the invention enables the determination of the mineral particle content in formulations, in particular selected from cosmetic products, pharmaceutical products, foodstuffs, paints and / or varnishes.

[0048] The method according to the invention can be used to determine the content of inorganic substances. For example, the content of calcium phosphates, titanium dioxide, zinc oxide, talc, kaolin, mica, iron oxides, ultramarine, tin oxide, zirconium dioxide, silicates, mixed silicates, layered silicates, carbonates, aluminum oxides, aluminum silicates, barium sulfate, chromium oxide green, cadmium sulfide, cinnabar, manganese violet, chromium oxide brown, bismuth oxide, or cobalt blue in various formulations can be determined.

[0049] According to the method, calcium phosphates from the group consisting of monocalcium phosphate monohydrate (MCPM), monocalcium phosphate anhydrous (MCPA), dicalcium phosphate dihydrate (DCPD, brushite), dicalcium phosphate anhydrous (DCPA, monetite), octacalcium phosphate (OCP), α-tricalcium phosphate (α-TCP), β-tricalcium phosphate (β-TCP), amorphous calcium phosphate (ACP; also as CPP-ACP complex = casein phosphopeptide - amorphous calcium phosphate), amorphous calcium phosphate (ACP), calcium-deficient hydroxyapatite (CDHA), hydroxyapatite (HA or HAP), tetracalcium phosphate (TTCP) and calcium pyrophosphate, preferably from the group consisting of monocalcium phosphate monohydrate (MCPM), monocalcium phosphate anhydrous (MCPA), dicalcium phosphate dihydrate (DCPD, brushite), dicalcium phosphate anhydrous (DCPA, monetite), octacalcium phosphate (OCP), amorphous calcium phosphate (ACP;also known as CPP-ACP complex = casein phosphopeptide - amorphous calcium phosphate), amorphous calcium phosphate (ACP), calcium-deficient hydroxyapatite (CDHA), hydroxyapatite (HA or HAP) and tetracalcium phosphate (TTCP) and any mixtures of these compounds, preferably hydroxyapatite.;

[0050] Preferably, the determination of the mineral particle content in oral care products selected from toothpastes, mouthwashes, oral gels or polishing pastes is used.

[0051] The determination of the total mineral content of toothpastes, and in particular the determination of hydroxyapatite in toothpastes, is preferred. A relative comparison of the hydroxyapatite contents of at least two toothpastes is especially preferred.

[0052] Toothpaste is a special paste or cream used to clean teeth and prevent tooth decay and gum disease.

[0053] An oral gel is a special gel-like substance used to treat gum inflammation, mouth ulcers, or other oral ailments, as well as to prevent tooth decay and other dental diseases. A mouthwash is a liquid used to clean the oral cavity, combat bad breath, and prevent tooth decay and gum disease.

[0054] A polishing paste is a special paste used to smooth and polish teeth and dental prostheses to achieve a smooth and aesthetically pleasing surface.

[0055] Also preferred is the determination of mineral particles in sun protection products selected from sunscreens, sun lotions, sun sprays, sun oils, or lip care products. The determination of titanium dioxide in sunscreens and sun lotions is particularly preferred.

[0056] Sunscreens are special creams that are applied to the skin to protect against sunburn and UV radiation.

[0057] Sunscreen is a liquid sun protection lotion that is applied to the skin to protect against sunburn and UV radiation.

[0058] Sun sprays are spray products that are applied to the skin to protect against sunburn and UV radiation.

[0059] Sun oils are oils that are applied to the skin to protect against sunburn and UV radiation.

[0060] Lip care products are special products that are applied to the lips to protect them from dryness, sunburn and UV radiation.

[0061] The determination of mineral particles in wall paints is also preferred.

[0062] Wall paint is a special type of paint used to coat interior and exterior walls to protect and decorate them.

[0063] A particularly preferred embodiment according to the invention relates to a method for

[0064] Determination of the hydroxyapatite content in a toothpaste, wherein the method comprises the steps (a) applying the toothpaste to a test matrix according to the invention, preferably test strips,

[0065] (a') Drying of the applied toothpaste and

[0066] (b) Determination of the hydroxyapatite content by comparison with reference values, in particular other toothpastes.

[0067] One aspect of the invention relates to a kit comprising a test matrix according to the invention, preferably at least one test strip according to the invention, instructions for carrying out the method according to the invention, and a formulation that can be analyzed according to the method according to the invention. A kit comprising at least one test strip according to the invention, instructions for carrying out the method according to the invention, and a dental care product, in particular toothpaste, is preferred. The kit enables the consumer to independently compare the mineral content, and in particular the hydroxyapatite content, of the toothpaste contained in the kit with other toothpastes.

[0068] Figures:

[0069] Fig. 1: Exemplary grey tone scale printed on paper, here Sigel laser paper,

[0070] LP207, DIN A4, white, printed with a Konica Minolta printer, type bizhub C450L

[0071] Fig. 2: The test strips show different coverages, depending on the toothpaste used.

[0072] Fig. 3: The determined mean grey values ​​of the test strips plotted against the hydroxyapatite content.

[0073] Fig. 4: Division of the grey tone chart into different rating ranges.

[0074] Fig. 5: Top: The test strips according to the invention with inorganic

[0075] The surfaces show a homogeneous coverage of the toothpaste formulations.

[0076] Below: The use of cellulose-containing cardboard as a test substrate leads to inhomogeneous coverage.

[0077] Fig. 6: The test strips show different coverage levels depending on the sunscreen used.

[0078] Fig. 7: The determined mean grey values ​​of the test strips plotted against the titanium dioxide content.

[0079] Examples

[0080] The invention is explained below using examples.

[0081] Example 1: Determination of the mineral content in toothpastes using the inventive device

[0082] Test strips

[0083] The following formulations are used to determine the total mineral content in toothpastes:

[0084] Toothpastes A to F are manufactured with the above-mentioned amounts of hydroxyapatite (HAP).

[0085] 0.3 g of each toothpaste A to F is distributed homogeneously onto the test strips, as shown in Figure 2. The toothpaste is placed on the test strip and spread towards the end of the strip.

[0086] The surface of the test strips consists of silicon carbide with a mean grain size of 201 pm. The coated test strips are completely dried with a hairdryer (AD31, Anruzon) and photographed together. As shown in Figure 2, the test strips exhibit different levels of coating, depending on the hydroxyapatite content. The coating ranges from no coating to complete coating.

[0087] The generated image containing the test strips to be evaluated is converted into an 8-bit grayscale image using image processing software (ImageJ, National Institutes of Health, Bethesda, USA). The software is then used to determine the average gray values ​​within the coverage area of ​​the test strips.

[0088] The mean gray values ​​are plotted against the hydroxyapatite content, as shown in Figure 3.

[0089] To verify the total mineral content of a test toothpaste, toothpastes Z1 to Z3, listed in the table above, are used. These test toothpastes also contain hydrated silica, a common mineral abrasive in toothpastes.

[0090] The test strips with toothpastes Z1 to Z3, containing total minerals of 3% wt., 7% wt., and 25% wt., are prepared and evaluated as described above. The gray values ​​of the test formulations from a five-part determination are listed in the following table:

[0091] The gray values ​​correspond to the reference curve from Fig. 3.

[0092] The example shows how the method according to the invention enables the determination of the total mineral content in toothpastes.

[0093] Example 2: Rapid test for determining the mineral content in toothpastes using a rating system

[0094] To use the method according to the invention as a rapid test, the total mineral content of toothpastes Z1 to Z3 from Example 1 is determined using a rating scale. The test strips containing toothpastes Z1 to Z3 from Example 1 are visually evaluated using a grayscale. The grayscale is printed on white paper (Sigel laser paper, LP207, DIN A4, white) using a printer (Konica Minolta, bizhub C450i).

[0095] The RGB values ​​refer to the numerical representation of the intensities of the primary colors red, green, and blue. The value "0" in Figure 4 represents the RGB value (0, 0, 0) and thus the color black. The value "255" in Figure 4 represents the RGB value (255, 255, 255) and thus the color white, or the base of the grayscale. All intermediate values ​​correspond to an even combination of the primary colors ("100" = (100 / 100 / 100)) and thus a shade of gray.

[0096] For the grey tone scale, according to Figure 4, an evaluation scheme is defined with the RGB ranges 0 to 100 (0 - 1 wt.% total mineral content, barely any coverage), 100 to 150 (1 - 5 wt.%, moderate coverage), 150 to 220 (5 - 10 wt.%, predominant coverage) and 220 to 255 (> 10 wt.%, complete coverage).

[0097] The test strips Z1 to Z3 are visually evaluated in a five-fold determination using the grey scale:

[0098] The example shows that the method according to the invention, in combination with the grey scale mentioned, is suitable for the quick and practical classification of the total mineral content in toothpastes.

[0099] Example 3: Comparison of different test surfaces to determine the

[0100] Total mineral content

[0101] In the following example, the test strips described above are compared with black, cellulose-based cardboard to test the suitability of a different surface.

[0102] The test strips from examples 1 and 2 are replaced with test strips made of black cardboard (2 x 10 cm, black, 300 g / m²). 2 , idee. Creativmarkt GmbH & Co. KG). For this purpose, the cardboard strips are prepared with the corresponding toothpaste formulations analogously to Example 1.

[0103] Surprisingly, when using black cardboard, as shown in Figure 5, an inhomogeneous, non-evaluable coating appears on a cellulose-containing surface.

[0104] The example shows that a homogeneous application is only possible with the characteristic features of the test strips according to the invention.

[0105] Example 4: Determination of the titanium dioxide concentration from sunscreen

[0106] The following formulations are used to determine the titanium dioxide content in sunscreen:

[0107] Sunscreens G to L are manufactured with the above-mentioned amounts of titanium dioxide (TiC>2).

[0108] 0.3 g of each of the sunscreens G to L are distributed homogeneously onto the test strips, as shown in Figure 6. During distribution, the sunscreen is placed on the test strip and spread out towards the end of the strip.

[0109] The surface area of ​​the test strips corresponds to the surface area from Example 1. The covered test strips are dried analogously to Example 1 and photographed together. Figure 6 shows different levels of coverage, depending on the titanium dioxide content. The coverage ranges from no coverage to complete coverage.

[0110] The generated image containing the test strips to be evaluated is converted into an 8-bit grayscale image using ImageJ. The software is then used to determine the mean gray values ​​within the coverage area of ​​the test strips. These mean gray values ​​from five separate measurements are plotted against the titanium dioxide content, as shown in Figure 7.

[0111] To verify the titanium dioxide content of a sunscreen, the sunscreens S1 to S3 listed in the table above are used.

[0112] The test strips containing sunscreens S1 to S3 with total mineral contents of 2% wt., 7% wt., and 15% wt. are applied as described above and evaluated. The gray values ​​from a five-fold determination of the test formulations are listed in the following table:

[0113] The gray values ​​correspond to the reference curve from Fig. 7.

[0114] The example shows how the method according to the invention enables the determination of the titanium dioxide content in sunscreen.

[0115] The invention specifically addresses the following points:

[0116] 1. A method for determining the content of mineral particles in a formulation with a viscosity in the range of 1 mPas to 200,000 mPas, the method comprising the steps

[0117] (a) Applying the formulation to a test matrix, preferably a test strip, wherein the surface of the test strip on which the formulation is applied has a defined roughness with a mean arithmetic height in the range of 0.1 to 500 pm,

[0118] (b) Determination and evaluation of the mineral content.

[0119] 2. Method according to point 1, wherein the surface of the test matrix on which the formulation is formed from inorganic material and / or applied by means of a printing process.

[0120] 3. Method according to any of the preceding points, wherein the test matrix comprises abrasive grains of silicon carbide, diamond, zirconium oxide or aluminium oxide on a support layer.

[0121] 4. Method according to point 2, wherein the printing method is selected from bubble printing, high pressure (relief printing), gravure printing, screen printing, UV spot varnishing, thermography, digital 3D printing, frost printing or flock printing.

[0122] 5. Method according to any of the preceding points, wherein the mean arithmetic height of the surface of the test matrix has a defined roughness with a mean arithmetic height in the range of 1 to 300 pm.

[0123] 6. Method according to one of the preceding points, wherein the test matrix is ​​formed from sandpaper.

[0124] 7. Method according to any of the preceding points, wherein the formulation in step (a) is applied to the test matrix using a finger, toothbrush, spatula, putty knife, brush, roller or sponge.

[0125] 8. A method according to any of the preceding points, wherein a drying step (a') is performed after the formulation has been applied to the test matrix according to step (a). A method according to any of the preceding points, wherein several formulations to be analyzed are applied to the test matrix in parallel. A method according to any of the preceding points, wherein the mineral content of a formulation is determined visually relative to a reference formulation. A method according to any of the preceding points, wherein the mineral content of at least two formulations is determined relative to each other, preferably visually. A method according to any of the preceding points, wherein the mineral content of a formulation after application to the test matrix is ​​less than 1 wt% without visible coverage of the test matrix, 1 to 5 wt% with moderate coverage of the test matrix, and 5 to 10 wt% with predominant coverage of the test matrix.-% and is greater than 10 wt% when the test matrix is ​​completely covered. A method according to any of the preceding points, wherein the evaluation of the mineral particle content after application of the formulation is carried out using a reference scale, preferably by determining the gray values. A method according to any of the preceding points, wherein the content of minerals selected from calcium phosphates, titanium dioxide, zinc oxide, talc, kaolin, mica, iron oxides, ultramarine, tin oxide, zirconium dioxide, silicates, mixed silicates, layered silicates, carbonates, aluminum oxides, aluminum silicates, barium sulfate, chromium oxide green, cadmium sulfide, cinnabar, manganese violet, chromium oxide brown, bismuth oxide and / or cobalt blue is determined. A method according to any of the preceding points, wherein the content of mineral particles in cosmetic products, pharmaceutical products, foodstuffs, paints or varnishes is determined.A method according to any of the preceding points, wherein the mineral particle content in oral care products is determined. A method according to any of the preceding points, wherein the hydroxyapatite content in toothpastes is determined. A method according to any of the preceding points, wherein the mineral particle content in sunscreens is determined. A method according to any of the preceding points, wherein the mineral particle content in wall paints is determined. A kit comprising instructions for carrying out the method according to any of points 1 to 18, a test matrix, preferably at least one test strip, and an oral care product, preferably a toothpaste.

Claims

Claims 1. A method for determining the content of mineral particles in a formulation with a viscosity in the range of 1 mPas to 200,000 mPas, the method comprising the steps (c) Applying the formulation to a test matrix, preferably a test strip, wherein the surface of the test strip on which the formulation is applied has a defined roughness with a mean arithmetic height in the range of 0.1 to 500 pm, (d) Determination and evaluation of the mineral content.

2. Method according to claim 1, wherein the surface of the test matrix on which the formulation is formed from inorganic material and / or applied by means of a printing process.

3. Method according to any of the preceding claims, wherein the test matrix comprises abrasive grains of silicon carbide, diamond, zirconium oxide or aluminium oxide on a support layer.

4. Method according to claim 2, wherein the printing method is selected from bubble printing, high pressure (relief printing), gravure printing, screen printing, UV spot varnishing, thermography, digital 3D printing, frost printing or flock printing.

5. Method according to any of the preceding claims, wherein the mean arithmetic height of the surface of the test matrix has a defined roughness with a mean arithmetic height in the range of 1 to 300 pm.

6. Method according to any of the preceding claims, wherein the formulation is applied to the test matrix in step (a) using a finger, toothbrush, spatula, putty knife, brush, roller or sponge.

7. Method according to one of the preceding claims, wherein after applying the formulation to the test matrix according to step (a) a drying step (a') is carried out.

8. Method according to one of the preceding claims, wherein several formulations to be analyzed are applied to the test matrix in parallel.

9. Method according to any of the preceding claims, wherein the mineral content of at least one formulation is determined visually relative to a reference formulation and / or of at least two formulations relative to each other.

10. Method according to any of the preceding claims, wherein the mineral content of a formulation after application to the test matrix is ​​less than 1 wt.% without visible coverage of the test matrix, is 1 to 5 wt.% with moderate coverage of the test matrix, is 5 to 10 wt.% with predominant coverage of the test matrix, and is greater than 10 wt.% with complete coverage of the test matrix.

11. Method according to one of the preceding claims, wherein the evaluation of the mineral particle content after application of the formulation is carried out using a reference scale, preferably by determining the grey values.

12. Method according to any one of the preceding claims, wherein the content of minerals selected from calcium phosphates, titanium dioxide, zinc oxide, talc, kaolin, mica, iron oxides, ultramarine, tin oxide, zirconium dioxide, silicates, mixed silicates, layered silicates, carbonates, aluminum oxides, aluminum silicates, barium sulfate, chromium oxide green, cadmium sulfide, cinnabar, manganese violet, chromium oxide brown, bismuth oxide and / or cobalt blue is determined.

13. Method according to any of the preceding claims, wherein the content of mineral particles in cosmetic products, pharmaceutical products, foodstuffs, paints or varnishes, preferably oral care products, sun protection products or wall paints, is determined.

14. Method according to one of the preceding claims, wherein the content of hydroxyapatite in toothpastes is determined.

15. Kit comprising instructions for carrying out the method according to any one of claims 1 to 14, a test matrix, preferably at least one test strip, and an oral care product, preferably a toothpaste.

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