Apoptosis detection

EP4747629A2Pending Publication Date: 2026-05-27LOUGHBOROUGH UNIV +1
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Patent Information

Authority / Receiving Office
EP · EP
Patent Type
Applications
Current Assignee / Owner
LOUGHBOROUGH UNIV
Filing Date
2024-07-19
Publication Date
2026-05-27

AI Technical Summary

Technical Problem

Current methods for detecting apoptotic cells, such as using Annexin V, require waiting several hours for phosphatidylserine to be exposed on the cell surface, making it difficult to detect apoptosis at earlier stages.

Method used

The method involves determining the level of extracellular and/or extravesicular surface display of specific glycans, including terminal sialic acid, galactose, mannose, N-acetylgalactosamine, fucose, and N-acetylglucosamine residues, using lectins like MAL-II, SNA, GNA, STL, AAL, UEA-I, SJA, PNA, and NPL, to detect apoptotic cells and/or vesicles.

Benefits of technology

This approach allows for the early detection of apoptotic cells, as soon as about 1 hour after apoptosis initiation, without the need for cell lysis or permeabilization, providing a more timely and efficient method compared to existing techniques.

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Abstract

The present invention provides a method of detecting the presence or absence of apoptotic cells and / or vesicles thereof in a sample, the method comprising: providing a sample; determining a level of extracellular and / or extravesicular surface display of a glycan in the sample; comparing the level of display of the glycan in the sample with a reference value; and detecting the presence of apoptotic cells and / or vesicles thereof, or detecting the absence of apoptotic cells and / or vesicles in the sample when based on the comparison.
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