Methods and systems for sequence deconvolution

Abstract

In a first aspect, the disclosure provide methods of identifying polynucleotide molecules distributed in a plurality of partitions, the method including providing a plurality of sequencing read data; providing a plurality of reference sequences; comparing each of the plurality of sequencing read data to each of the plurality of reference sequences using an algorithm; generating, based on the comparison of each of the plurality of sequencing read data to each of the plurality of reference sequences, a score for each of the sequencing read data; and assigning to each of the plurality of sequencing read data, based on the score for each of the sequencing read data, a polynucleotide molecule, thereby identifying each polynucleotide molecule in each of the plurality of partitions.

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