Sperm cell isolation device and method for selecting high-quality sperm cells

FR3136479B1Active Publication Date: 2025-08-29BÉEZ BIOTECH
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Patent Information

Application Number
FR2022005675
Authority / Receiving Office
FR · FR
Patent Type
Patents
Current Assignee / Owner
Filing Date
2022-06-13
Publication Date
2025-08-29
Estimated Expiration
2042-06-13

AI Technical Summary

Technical Problem

Current sperm selection methods, such as those outlined by the World Health Organization (WHO) and density gradient centrifugation, are inadequate for selecting high-quality sperm cells due to DNA fragmentation and suboptimal consideration of physiological conditions, leading to low success rates in artificial reproductive technologies (ART) like ICSI, IUI, and IVF.

Method used

A microfluidic device that mimics the natural selection process by combining rheotaxis and thermotaxis mechanisms, utilizing a hydrostatic pressure difference and temperature gradient to isolate high-quality sperm cells, enhancing sperm cell migration and selection efficiency.

Benefits of technology

The device achieves a high-yield collection of sperm cells with 100% motility and DNA integrity, surpassing the capabilities of existing methods by improving sperm cell recovery and throughput.

✦ Generated by Eureka AI based on patent content.

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Abstract

The present invention relates to a sperm cell selection device having a support plate comprising an inlet for receiving a sperm cell sample, an outlet for collecting at least a portion of the sperm cells comprised in said sample, and a microfluidic system between said inlet and outlet, wherein the microfluidic system comprises a cuvette arranged to receive a medium and having an inlet area and an outlet area respectively proximate the inlet and the outlet, and wherein the inlet and the outlet are arranged to provide a hydrostatic pressure difference between said inlet area and said outlet area when a medium is added to the cuvette via the outlet,so as to cause a flow of said medium from the outlet area toward the inlet area to initiate sperm cell migration of the sperm cells of the sperm cell sample from the inlet area toward the outlet area, and wherein the inlet area and the outlet area are in fluid communication with each other via a main channel arranged in the cuvette, the channel having a reduced width area arranged between the inlet area and the outlet area so as to form a rheotaxis zone, whereby said sperm cell sample undergoes selection by passing through said rheotaxis zone during said sperm cell migration thereby enabling a quality sperm cell sample to be collected from the outlet area at the outlet. Figure for abstract: Fig. 1,
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