Generic and sterile sensor system on a mobile mast

Abstract

The invention relates to an analysis device (1) for at least one parameter of a chemical, biochemical, or biological reaction (2) in a reaction chamber (3), the analysis device (1) comprising: an arm (4) intended to be fixed by means of a sliding joint (41) against a face (300) of a wall (30) of the reaction chamber (3) by means of a fixed end (40), the arm (4) having one degree of freedom in translation relative to the wall (30); a sensor (5) configured to measure at least one parameter, the sensor (5) being fixed to a free end (42) of the arm (4); a motorization device (7) configured to induce an axial displacement of the arm (4) according to the degree of freedom; a hermetic isolation device (8) of the arm (4) with respect to the reaction (2), the hermetic isolation device (8) connecting the arm (4) to the face (300) of the wall (30). Figure for the abridged version: Fig. 1

Description

Title of the invention: Generic and sterile sensor system on a mobile mast

[0001] The invention relates to the fields of pharmaceutical, cosmetic, biomass production or agri-food industries.

[0002] In particular, the invention relates to a device for analyzing and monitoring at least one parameter of a chemical, biochemical or biological reaction in a reaction chamber.

[0003] The multiplication or culture of microorganisms is widely used in the pharmaceutical, cosmetics and food industries for the production of biomass, for the production of a metabolite or the bioconversion of a molecule of interest, but also in winemaking and brewing.

[0004] This multiplication is advantageously carried out in a bioreactor which allows control of culture conditions, and more particularly of reaction parameters such as temperature, pH, oxygen level or biomass growth rate.

[0005] A bioreactor, as is known, comprises a reaction chamber in which a stirrer and / or an aerator are generally arranged to homogenize and oxygenate the reaction medium, respectively. The bioreactor further comprises a thermal jacket that allows for the imposition of thermalization on the reaction medium. Such a bioreactor is also equipped with sensors, for example, temperature, pH, and dissolved oxygen sensors, to monitor the state of the culture and, if necessary, adjust the culture conditions within the reaction chamber, preferably in real time.

[0006] However, these sensors are generally mounted in a fixed manner on rods immersed in the reaction medium.

[0007] However, this sensor mounting has many disadvantages.

[0008] Indeed, the fixed sensors only probe the reaction medium locally, that is, in the vicinity of the sensors themselves, and do not detect any potential inhomogeneity in the reaction medium occurring at a distance from the sensors. The data collected by these sensors therefore do not allow for optimal adjustment of the reaction parameters.

[0009] Therefore, in order to better account for the inhomogeneity of the reaction medium, it was considered to use a "movable sensor". Such a sensor can, in particular, be housed in a capsule immersed in the reaction medium. The capsule is then subjected to the currents imposed by the stirrer and is thus exposed to different areas of the medium. reactive.

[0010] However, these capsules have the disadvantage, since their movement in the bioreactor is free, of regularly coming into contact with the walls of the bioreactor or the blades of the agitator, and consequently of being damaged.

[0011] Moreover, the path of the capsules in the reaction medium remains random, and does not allow to cover the entire extent of the bioreactor.

[0012] Thus, as disclosed in document EP 3 967 743 A1, it was envisaged to control the movement of the movable sensor by means of a mast fixed against two opposite walls of the reaction chamber. The analysis kit described in document EP 3 967 743 A1 therefore proposes a hollow mast fixed against two opposite walls of the reaction chamber, against which a movable sensor is attached via a magnetic link. The sensor is then free to move in translation along a transverse axis closed by the mast.

[0013] However, such an installation presents new drawbacks. Indeed, there are two main types of bioreactors: stainless steel or glass bioreactors and single-use flexible bioreactors (SUBs). The main difference is that stainless steel or glass bioreactors can be used many times and therefore require complex cleaning (acid / base) to eliminate all organisms before the next production run, whereas single-use flexible bioreactors are disposable after a single use and have the advantage of guaranteeing sterility without cleaning. Single-use flexible bioreactors currently represent approximately 80% of production for the pharmaceutical industry. These disposable bioreactors have a less rigid supporting structure compared to stainless steel or glass bioreactors.

[0014] Consequently, disposable bioreactors are generally unsuitable for receiving such a device, as disclosed in document EP 3 967 743 AL. More specifically, the walls of the reaction vessels of single-use flexible bioreactors are not designed for the double attachment of the mast and therefore require modification to incorporate the mast. It is thus complex to adapt such an analytical device for single-use flexible bioreactors by adding a mounting at the top and a mounting at the bottom of the reaction vessel.

[0015] Furthermore, such a device presents sterility problems when the system is inserted into the bioreactor.

[0016] The invention aims to overcome all or part of the problems mentioned above by proposing a generic and sterile sensor system integrated on a mobile mast for measuring physiological or physical quantities over the entire height of a bioreactor, the mast being easily integrated into any reaction vessel. The analysis device according to the invention also has the advantage of allowing the sensor and the mobile mast to be introduced in a hermetically sealed manner and without impacting the reaction medium.

[0017] To this end, the invention relates to a device for analyzing at least one parameter of a chemical, biochemical, or biological reaction in a reaction chamber, the analytical device comprising: - an arm intended to be fixed by means of a sliding connection against a face of a wall of the reaction chamber by means of a fixed end, the arm having a degree of freedom in translation relative to the wall of the reaction chamber; - a sensor configured to measure at least one parameter, the sensor being fixed to a free end of the arm; - a motorization device configured to induce an axial displacement of the arm according to the degree of freedom; - a hermetic isolation device for the arm from the chemical, biochemical or biological reaction, the hermetic isolation device connecting the arm to the face of the wall of the reaction chamber.

[0018] According to one aspect of the invention, the hermetic insulation device comprises a material among silicone, a fluorinated elastomer, polyetheretherketone, ethylene-propylene-diene monomer, polytetrafluoroethylene.

[0019] According to one aspect of the invention, the airtight insulation device includes a bellows.

[0020] According to one aspect of the invention, the wall comprises an inner face facing the reaction, the hermetic insulation device being arranged so as to connect the arm to the inner face of the wall.

[0021] According to one aspect of the invention, the wall comprises an external face facing an external environment to the reaction, the hermetic insulation device being arranged so as to connect the arm to the external face of the wall.

[0022] According to one aspect of the invention, the analysis device includes a means for controlling the arm, connected to the motorization device and arranged to control the movement of the arm according to the degree of freedom.

[0023] According to one aspect of the invention, the analysis device includes a means for transmitting data that can be measured by the sensor.

[0024] According to one aspect of the invention, the analysis device includes a device for recording the data measured by the sensor.

[0025] According to one aspect of the invention, the sensor is a sensor capable of measuring a data point among pH, dissolved oxygen, carbon dioxide, electrical conductivity, redox potential or temperature.

[0026] According to one aspect of the invention, the arm is a telescopic arm.

[0027] According to one aspect of the invention, the hermetic insulation device comprises a seal positioned between the bellows and the face of the wall of the reaction chamber, the seal comprising a first sealing surface in contact with the face of the wall of the reaction chamber and a second sealing surface in contact with the bellows.

[0028] The invention also relates to a reaction chamber for a chemical or biochemical or biological reaction comprising the device for analyzing at least one parameter of the chemical or biochemical or biological reaction in the reaction chamber.

[0029] The invention will be better understood and other advantages will become apparent upon reading the detailed description of an embodiment given by way of example, a description illustrated by the accompanying drawing in which:

[0030] [Fig-1] [Fig.1] represents a schematic view of an analysis and monitoring device according to the present invention;

[0031] [Fig.2] [Fig.2] represents a schematic view of the analysis and monitoring device in a preferred architecture;

[0032] [Fig.3] [Fig.3] represents a schematic view of a variant of the device analysis and monitoring of the [Fig.2];

[0033] [Fig.4] [Fig.4] represents a schematic view of a reaction chamber comprising two analysis devices according to the invention;

[0034] [Fig.5A] [Fig.5A] represents a schematic view of the analysis and monitoring device according to a first configuration mode in which a hermetic insulation device is extended;

[0035] [Fig.5B] [Fig.5B] represents a schematic view of the analysis and monitoring device of [Fig.5A] in which the hermetic insulation device is compressed;

[0036] [Fig.6A] [Fig.6A] represents a schematic view of the analysis and monitoring device according to a second configuration mode in which the hermetic isolation device is extended;

[0037] [Fig.6B] [Fig.6B] represents a schematic view of the analysis and monitoring device of [Fig.6A] in which the hermetic insulation device is compressed;

[0038] [Fig.7] [Fig.7] represents a detailed view of the analysis and monitoring device figures 6A and 6B;

[0039] [Fig. 8] [Fig. 8] shows a detailed view of the attachment of the device arm analysis and monitoring according to the invention.

[0040] For the sake of clarity, the same elements will bear the same references in the different figures.

[0041] Fig. 1 represents a schematic view of an analysis and monitoring device 1 of at least one parameter of a chemical or biochemical or biological reaction 2 in a reaction chamber 3.

[0042] The analysis device 1 comprises an arm 4 intended to be fixed by means of a sliding joint 41 against a face 300 of a wall 30 of the reaction chamber 3 by means of a fixed end 40. The arm 4 has one degree of freedom in translation relative to the wall 30 of the reaction chamber 3. In other words, the arm 4 is configured to extend axially relative to the wall 30 and locally relative to the face 300 along a first axis AL. According to a preferred aspect of the invention, the arm 4 translates substantially perpendicularly relative to the face 300 of the wall 30.

[0043] Alternatively, the arm 4 is able to translate along an axis secant with respect to the wall 300. This axis of translation can be arbitrary as long as the arm does not come into contact in an undesired manner with a component such as the agitator of the bioreactor.

[0044] The arm 4 is therefore in sliding connection with the face 300 of the wall 30 of the reaction chamber 3 so as to be able to be guided along the first axis Al in translation.

[0045] The analysis device 1 also includes a sensor 5 configured to measure at least one parameter of the reaction 2, the sensor 5 being fixed to a free end 42 of the arm 4 in contact with the reaction 2. This free end 42 is a moving end due to the translational mobility of the arm 4. The free end 42 is, therefore, substantially opposite the face 300 of the wall 30. In addition, the sensor 5 is thus intended to collect data from a reaction medium in which the sensor 5 is likely to be immersed.

[0046] The analysis device 1 may also include several sensors 5 fixed to the free end 42 of the arm 4 so as to measure several parameters of the reaction 2.

[0047] In particular, the data collected by the sensor(s) 5 may relate to the experimental conditions imposed on the reaction medium. These conditions may be characteristic of acidity, agitation, and temperature. The data collected may also relate to the progress of reaction 2 or to the phenomena occurring in the reaction medium.

[0048] By way of example, the sensor(s) 5 may include at least one of the following elements: a pH measurement sensor, a temperature measurement sensor, a sensor for measuring the quantity of dissolved oxygen, a sensor for measuring the quantity of carbon dioxide or dissolved oxygen, a sensor capable of measuring electrical conductivity or redox potential, i.e. the oxidation-reduction reaction occurring within reaction 2. The invention is not limited to these sensors alone, and a person skilled in the art, depending on the reaction parameters to be monitored, may implement any other sensor.

[0049] The analysis device 1 also includes a data transmission means 6 for data that can be measured by the sensor 5. The data transmission means 6 thus makes it possible to transmit all the data collected by the sensor 5 to a platform enabling real-time analysis of the evolution of the reaction medium.

[0050] Furthermore, the analysis device 1 includes a drive device 7 configured to induce the axial displacement of the arm 4 according to its degree of freedom. In other words, the drive device 7 makes it possible to induce the translation of the arm 4, and therefore of the sensor 5, along the first axis Al within the reaction chamber 3. In addition, the drive device 7 may, according to a preferred configuration, include a guide channel 70 for the arm 4, through which the arm 4 passes during its translations along the first axis Al, thus channeling the movement of the arm 4.

[0051] Alternatively, the arm 4 can also be guided during its translations by means of a double gimbal or a flexible silent-block type connection in order to avoid over-constraining the translational movement. This assembly makes it possible to maintain good alignment of the arm 4, to resist the radial load in the connection between the arm 4 and the motorization device 7 due to the pressure of the reaction 2 on the arm 4, and to guarantee a seal between the inside of the hermetic insulation device 8 and the reaction 2 in order to prevent any contamination.

[0052] By way of indicative example, the motorization device 7 may be a ball screw motor, pantograph or linear actuator.

[0053] Alternatively, any motorized device enabling the induction of a translational movement may be considered.

[0054] Furthermore, the analysis device 1 includes a hermetic isolation device 8 for the arm 4 with respect to the chemical, biochemical, or biological reaction 2. The hermetic isolation device 8 is arranged to connect the arm 4 to the face 300 of the wall 30 of the reaction chamber 3. The hermetic isolation device 8 thus makes it possible to isolate the arm 4 from the reaction 2 within the reaction chamber 3. Consequently, the sterility of the reaction 2 is guaranteed even when the arm 4 is moving within the reaction chamber 3.

[0055] According to the invention, the hermetic isolation device 8 is arranged to prevent the introduction of any foreign body into reaction 2 through an opening 400 formed at the fixed end 40 during the translation of the arm 4. Indeed, when the arm 4 is translated towards a face opposite to the face 300 of the wall 30, thus representing an insertion movement of the arm 4 and the sensor 5 into the reaction chamber 3, it is possible that a foreign body could become attached to the arm 4 and pass through the wall 30 of the reaction chamber 3 at the opening 400, come into contact with the reaction medium, and contaminate the ongoing reaction 2. Therefore, the hermetic isolation device 8 ensures a sealing zone between the arm 4 and reaction 2 in any translation situation along the first axis AL

[0056] According to a preferred configuration, the hermetic insulation device 8 comprises a first sealed end 80 connected to the free end 42 and a second watertight end 82 connected to face 300 of wall 30 around fixed end 40 for example.

[0057] According to the invention, the hermetic insulation device 8 comprises a material among silicone, a fluorinated elastomer, polyetheretherketone (PEEK), ethylene-propylene-diene monomer (EPDM), polytetrafluoroethylene (PTFE) or any other biocompatible material with the reaction 2 so as not to impact the reaction medium.

[0058] According to a preferred configuration, the hermetic insulation device 8 comprises a bellows 84, as shown in [Fig. 2]. The bellows 84 has the advantage of being adaptable to each position of the arm 4. Indeed, a bellows 84 has an elastic and extensible structure in the form of a bulge 840, allowing it to be stretched by extending each bulge 840 and compressed by compressing each bulge 840. The use of a bellows 84 adapted to the movement of the arm 4 thus has the advantage of not impacting the reaction 2 during the movements of the arm 4 and the sensor 5, for example, by generating unwanted local disturbances and current movements in the reaction 2. Furthermore, such a type of bellows 84 has the advantage of being resistant to the numerous translations of the arm and therefore to the tensile and compressive stresses to which it is subjected.

[0059] By way of indicative example, the bellows 84 can be of cylindrical, rectangular or conical cross-section.

[0060] Alternatively, the hermetic insulation device 8 includes an elastic cylindrical pocket capable of withstanding the tensile and compressive stress exerted by the translation of the arm 4.

[0061] Advantageously, the analysis device 1 may also include a control means 9 for the arm 4 arranged to control the movement of the arm 4 according to its degree of freedom. More specifically, the control means 9 is connected to the drive device 7 such that the drive device 7 induces a translation of the arm 4 and the sensor 5 along the first axis A1 in response to a command from the control means 9. The control means 9 thus provides control of the arm 4. By way of example, the typical linear translation speed of the arm 4 is on the order of 0.01 m / s to 1.5 m / s and the spatial resolution is on the order of 0.1 mm to 1 cm depending on the type of motor or linear actuator used as the drive device 7.

[0062] The control means 9 can then allow the successive translation(s) of the arm 4 in the reaction chamber 3 to be configured in a predefined manner or be controlled in real time by a user.

[0063] The analysis device 1 may also include a recording device 10 for the data measured by the sensor 5. The recording device 10 then communicates with the sensor 5 via the data transmission means 6. In addition, the data transmission means 6 can be a wireless transmission means 60 of type wifi, ZigBee or Bluetooth for example.

[0064] Alternatively, as shown in [Fig.3], the data transmission means 6 can be a wired transmission means 62 linking the sensor 5 to the recording device 10 of type RS432, RS485, or RJ45 for example.

[0065] The analysis device 1 may also include a power supply 11 configured to power the control means 9 and the motorization device 7.

[0066] Advantageously, the hermetic isolation device 8 may also include a seal 85 positioned between the bellows 84 or the cylindrical pouch and the face 300 of the wall 30 of the reaction vessel 3. Indeed, the seal 85 comprises a first sealing surface 850 in contact with the face 300 of the wall 30 of the reaction vessel 3 and a second sealing surface 852 in contact with the bellows 84 or with the cylindrical pouch. The seal 85 thus provides additional insulation at the opening 400.

[0067] Alternatively, the seal 85 can be a scraper seal comprising a flexible inner lip bearing against the arm 4 and an outer lip fixed to the wall 30 of the reaction vessel 3. More specifically, the inner lip is pressed against the arm 4 so as to ensure a seal from the scraper seal during the sliding of the arm 4. In addition, the scraper seal can also comprise several overlapping inner lips pressed against the arm 4 to improve the sealing of the scraper seal.

[0068] It can also be envisaged, as represented in [Fig.4], to use several analysis devices 1. Indeed, the use of an arm 4 capable of translating along the first axis Al, limits the data acquisition of the sensor 5 to a height range, in the illustrated case, without being able to observe the reaction medium along a second axis A2 at least secant and preferably perpendicular to the first axis Al along a plane formed by the first axis Al and the second axis A2.

[0069] The analysis device 1 may then include: - a second arm 4' intended to be fixed to a second face 300' of the wall 30 of the reaction vessel 3 by means of a fixed end 40'. The second arm 4' also has a degree of freedom in translation relative to the wall 30 of the reaction vessel 3. Therefore, the second arm 4' translates along the second axis A2. - a second 5' sensor configured to measure at least one parameter, fixed to a free end 42' of the second arm 4', - a second means of transmitting data 6' that can be measured by the sensor 5' of the second arm 4', - a second 7' motorization device configured to induce a de- axial placement of the second arm 4' according to the degree of freedom of the second 4' swashplate and therefore according to the second axis A2, - and a second hermetic isolation device 8' of the second arm 4' with respect to the chemical or biochemical or biological reaction 2, configured to connect the second arm 4' to the second face 300' of the wall 30 of the reaction chamber 3.

[0070] The first face 300 and the second face 300' are two different faces of the wall 30 of the reaction chamber 3 and, preferably, are two faces extending perpendicularly or at least secantly.

[0071] Alternatively, the second axis A2 can also be secant to the first axis Al in the plane formed by the first axis Al and by the second axis A2.

[0072] In order not to limit the movements of the arm 4 and the second arm 4', it is desirable that the arm 4 and the second arm 4' be distant from each other along an axis perpendicular to the first axis Al and the second axis A2.

[0073] Alternatively, it may be envisaged to use more than two arms having a translational degree of freedom in the analysis device 1.

[0074] In the following description, only one arm 4 is mentioned. However, it may be envisaged to also use the second arm 4' or even more than two arms.

[0075] The arm 4 can advantageously be a telescopic arm. A telescopic arm has the advantage of being compact.

[0076] According to a first configuration mode shown in [Fig. 5A] and 5B, the hermetic isolation device 8 can be included inside the reaction chamber 3. Therefore, the hermetic isolation device 8, according to this first configuration, is arranged so as to connect the arm 4 to an inner face 301 of the wall 30 of the reaction chamber 3, the inner face 301 being opposite the reaction 2. And, similarly, the seal 85 is arranged between the bellows 84 or the cylindrical pocket and the inner face 301 of the wall 30.

[0077] More specifically, in [Fig.5A], the hermetic isolation device 8 is extendable along a stroke C in the reaction chamber 3.

[0078] Furthermore, when the arm 4 translates along the first axis Al so as to compress the airtight insulation device 8, as shown in [Fig. 5B]. However, this translation is limited up to a high position Hl, a position to which the bellows 84 or the elastic pocket cannot be compressed further.

[0079] Therefore, there is a dead zone Ml which represents an area for which the sensor 5 cannot detect information and observe the parameter(s) to be studied.

[0080] Thus, a second configuration of the analysis device 1 can be envisaged, as represented in [Fig.6A] and in [Fig.6B].

[0081] In this configuration, the hermetic insulation device 8 is arranged at the outside of the reaction chamber 3. More specifically, the hermetic isolation device 8 is arranged so as to connect the arm 4 to an external face 302 of the wall 30 of the reaction chamber 3, the external face 302 being in view of an environment external to the reaction 2 and opposite to the internal face 301. And, similarly, the seal 85 is arranged between the bellows 84 or the cylindrical pocket and the external face 302 of the wall 30.

[0082] This second configuration, with the hermetic isolation device 8 located outside the reaction chamber 3, has the advantage of increasing the stroke C of the arm 4 and thus allowing the sensor 5 to observe the parameter(s) over a greater height range, in the illustrated example.

[0083] In order to allow the bellows 84 or the elastic pouch to maintain a natural shape during arm movements 4, the bellows 84 or the elastic pouch can be coupled to a vent 86 allowing any overpressure to be evacuated inside the hermetic isolation device 8. The vent 86 can also be associated with a 0.2 pm filter to allow atmospheric pressure without allowing any contaminant to enter the hermetic isolation device 8.

[0084] Furthermore, the analysis device 1 according to the invention has the advantage of requiring only a single mounting port, namely the opening 400 on the face 300 of the wall 30, for the insertion of the arm 4 and the sensor 5, and of being suitable for mounting against the standard Clamp DN50 or DN70 mounting port of the reaction chambers 3 currently available on the market. The standard reaction chambers 3 therefore do not need to be adapted to the analysis device 1.

[0085] Figure 7 shows a detailed view of the analysis and monitoring device 1 for at least one parameter of the chemical, biochemical, or biological reaction 2 in the reaction chamber 3, according to the configuration shown in Figure 6A and Figure 6B. As stated previously, the arm 4 has one degree of freedom in translation relative to the wall 30 of the reaction chamber 3, such that the arm 4 translates substantially perpendicularly to the face 300 of the wall 30. This translation can, for example, be achieved by means of a translation guide 45 connecting the drive device 7 and the arm 4. Preferably, the translation guide 45 is a rail connected to the arm at a mounting 450. The mounting 450 is then movable on the rail, namely the translation guide 45, and the arm 4 is fixed to it by means of a fixed connection, for example.

[0086] Alternatively, as shown in [Fig.8], the fixing 450 may include a double cardan joint 452 thus ensuring good alignment of the arm 4 and preventing any overhang.

[0087] Furthermore, the sensor 5 can also be attached to the free end 42 of the arm 4 by means of a flexible and waterproof connector such as, for example, a PG 13.5 type connector.

Claims

Demands

1. Analytical device (1) for measuring at least one parameter of a chemical, biochemical, or biological reaction (2) in a reaction chamber (3), the analytical device (1) comprising: - an arm (4) for being fixed by means of a sliding joint (41) against a face (300) of a wall (30) of the reaction chamber (3) by means of a fixed end (40), the arm (4) having one degree of freedom in translation relative to the wall (30) of the reaction chamber (3); - a sensor (5) configured to measure at least one parameter, the sensor (5) being fixed to a free end (42) of the arm (4); - a drive device (7) configured to induce an axial displacement of the arm (4) according to the degree of freedom;- a hermetic isolation device (8) of the arm (4) with respect to the chemical, biochemical or biological reaction (2), the hermetic isolation device (8) connecting the arm (4) to the face (300) of the wall (30) of the reaction chamber (3).;

2. Analytical device (1) according to claim 1, wherein the hermetic isolation device (8) comprises a material among silicone, a fluorinated elastomer, polyetheretherketone (PEEK), ethylene-propylene-diene monomer (EPDM), polytetrafluoroethylene (PTFE).

3. Analytical device (1) according to claim 1 or 2, wherein the hermetic isolation device (8) includes a bellows (84).

4. Analytical device (1) according to any one of claims 1 to 3, wherein the wall (30) comprises an inner face (301) facing the reaction (2), the hermetic isolation device (8) being arranged so as to connect the arm (4) to the inner face (301) of the wall (30).

5. Analytical device (1) according to any one of claims 1 to 3, wherein the wall (30) comprises an external face (302) facing an external medium to the reaction (2), the hermetic isolation device (8) being arranged so as to connect the arm (4) to the external face (302) of the wall (30).

6. Analytical device (1) according to any one of claims 1 to 5, including a control means (9) for the arm (4), connected to the motorization device (7) and arranged to control the movement of the arm (4) according to the degree of freedom.

7. Analysis device (1) according to any one of the preceding claims, comprising a means for transmitting data (6) capable of being measured by the sensor (5).

8. Analysis device (1) according to any one of the preceding claims, comprising a device for recording (10) the data measured by the sensor (5).

9. Analytical device (1) according to any one of the preceding claims, wherein the sensor (5) is a sensor capable of measuring a data point among pH, dissolved oxygen (O2), carbon dioxide (CO2), electrical conductivity, redox potential or temperature.

10. Analytical device (1) according to any one of the preceding claims, wherein the arm (4) is a telescopic arm.

11. Analytical device (1) according to claim 3, wherein the hermetic isolation device (8) comprises a seal (85) positioned between the bellows (84) and the face (300) of the wall (30) of the reaction chamber (3), the seal (85) comprising a first sealing surface (850) in contact with the face (300) of the wall (30) of the reaction chamber (3) and a second sealing surface (852) in contact with the bellows (84).

12. Reaction chamber (3) for a chemical or biochemical or biological reaction comprising the analysis device (1) for at least one parameter of the chemical or biochemical or biological reaction (2) in the reaction chamber (3) according to any one of the preceding claims.