Extraction system for a functional peptide extract and product containing the latter

Abstract

Extraction System for a Functional Peptide Extract and Product Containing Thereto. The present invention relates to an extraction system for a functional peptide extract, said extraction system comprising a raw material reservoir (110), a solvent reservoir (120), a mixing reservoir (130), a high-temperature, high-pressure extraction device (140), and a filtration device (150), for extracting a functional peptide extract from mollusc shells and disposing of said peptide extract in a container body as a product containing a peptide extract for use by a user. Figure to be published with the abstract: Figure 1

Description

Title of the invention: Extraction system for a functional peptide extract and product containing the same Field of the invention

[0001] The present invention relates to an extraction system, in particular an extraction system for obtaining a functional peptide extract from mollusc shells and a product containing this extract. Background of the invention

[0002] Oysters are known as the "milk of the ocean." Taiwan has been farming oysters for over a century, with an annual yield of over 30,000 tons and a production value of 3.2 billion New Taiwan dollars. It is a very important contributor to Taiwan's fishing industry, both in quantity and value. However, oyster shells have long been treated as waste that is difficult to handle. Most oyster shells are discarded arbitrarily, which not only takes up space but also attracts mosquitoes and causes odor due to sun exposure, leading to environmental pollution to a certain extent.

[0003] Some industries have researched the recycling and reuse of oyster shells, such as the use of oyster shells in soil conditioners or poultry feed; however, the added value is not high. As such, inventing a system that effectively utilizes mollusk shells such as oyster shells for economic benefits and environmental protection is an important issue. Summary of the invention

[0004] To solve the above-mentioned problem, the purpose of the present invention is to provide an extraction system for obtaining, from mollusk shells, a functional peptide extract of commercial value, and to provide a product containing this extract. This functional peptide extract has the effects of whitening, repairing UV damage and anti-aging.

[0005] To achieve the above object, there is provided, according to the present invention, an extraction system for a functional peptide extract, comprising: a raw material tank for storing a mollusk shell powder; a solvent tank for storing a solvent; a mixing tank connected to both the raw material tank and the solvent tank, the mollusk shell powder and the solvent flowing into the mixing tank and being mixed therein to form a mixture; a high-temperature and high-pressure extraction device; pressure connected to the mixing tank, the mixture flowing into the high temperature and high pressure extraction device and being extracted therein under high temperature and high pressure conditions to obtain a crude extract; and a filtration device connected to the high temperature and high pressure extraction device for filtering the crude extract to isolate a peptide extract.

[0006] In a particular embodiment, the mollusk shell is obtained from mollusks having a layer of nacre, including oysters, clams, baby abalones, scallops, abalones, green mussels, golden clams or venus clams.

[0007] In a particular embodiment, the shell powder has a particle size between 20 qm and 100 qm.

[0008] In a particular embodiment, the solvent comprises water, the shell powder and water having a mixing ratio of 1:0.8.

[0009] In a particular embodiment, the high temperature and high pressure condition is a temperature between 110°C and 400°C, a pressure between 101325 Pa and 253312.5 Pa (1 and 2.5 atm), and a heating time between 2 and 4 hours.

[0010] In a particular embodiment, the high temperature condition is a temperature between 130°C and 300°C.

[0011] In a particular embodiment, the system further comprises a grinding device connected to the raw material tank for reducing the mollusk shells to powder and transporting them into the raw material tank.

[0012] In a particular embodiment, the system further comprises a cleaning device connected to the grinding device for cleaning the mollusk shells and transporting them into the grinding device.

[0013] In a particular embodiment, the peptide extract has at least one of the effects of decreasing melanin production, decreasing the effects of tyrosinase, repairing skin cells damaged due to UVA, promoting the production of type I procollagen, and decreasing breakdown by the enzyme MMP-1.

[0014] According to another aspect of the present invention, there is provided a product containing a functional peptide extract, comprising: a container body having a container chamber and an opening on top thereof; a peptide extract disposed inside the container chamber, the peptide extract being obtained from the aforementioned extraction system; and a lid for covering the opening of the container body.

[0015] In conclusion, the present invention will be explained in more detail by specific embodiments indicated below. The embodiments are merely listed as examples, and do not limit the scope of protection of the present invention. A person skilled in the art can readily understand that the non-key factors can be modified or adjusted to achieve equivalent effects. Brief description of the drawings

[0016] [Fig.l] is a block diagram of an extraction system for a functional peptide extract according to one embodiment of the present invention.

[0017] [Fig.2] is a functional diagram of an extraction system for an extract functional peptide according to another embodiment of the present invention.

[0018] [Fig.3] is a functional diagram of an extraction system for an extract functional peptide according to yet another embodiment of the present invention.

[0019] [Fig.4] is an isometric diagram of a product containing a peptide extract functional of the present invention. Detailed description of the embodiments

[0020] The implementation of the present invention is explained below according to Figures 1 to 4. The description is an example of the embodiments of the present invention, and is not intended to limit the implementation of the present invention. Furthermore, the same or similar reference numerals in the figures and embodiments are parts or elements that are the same, equivalent, or have the same function. The figures are all simplified and are not drawn to scale in all embodiments.

[0021] Referring to [Fig.l], a block diagram of an extraction system for a functional peptide extract according to one embodiment of the present invention is shown.The extraction system for a functional peptide extract 100 of the present invention comprises: a raw material tank 110 for storing a mollusk shell powder; a solvent tank 120 for storing a solvent; a mixing tank 130 connected to both the raw material tank 110 and the solvent tank 120, the shell powder and the solvent flowing into the mixing tank 130 and being mixed therein to form a mixture; a high-temperature and high-pressure extraction device 140 connected to the mixing tank 130, the mixture flowing into the high-temperature and high-pressure extraction device 140 and being extracted under high-temperature and high-pressure conditions to obtain a crude extract; and a filtration device 150 connected to the high-temperature and high-pressure extraction equipment 140 for filtering the crude extract to isolate a peptide extract.The peptide extract can be incorporated into creams, ointments, cosmetics or skin care products.

[0022] The isolated peptide extract of the present invention has the following effects:

[0023] (1) Whitening:

[0024] (A) Decrease in melanin production: Using melanoma cells of B16F10 mice as an experimental model, melanin production was decreased by 36% when a peptide extract of concentration 4mg / mL was added, which was close to a negative control group consisting of adding 10 μg / mL of Arbutin; melanin production was decreased by 49% when a peptide extract of concentration 8mg / mL was added. The experimental results are shown in Table 1 below: Group Positive control group Negative control group Experimental group Added peptide extract (mg / mL) 2 4 8 16 Cell activity (%) 100+26 84+22 86+28 83+19 60+8 9.0+10 Melanin (%) 100+3 62+0.8 80+4.0 65+0.8 51+2.6 60+6.5

[0025] Table 1: Effects on B16F10 cell activity and melanin production after stimulation with 10 nM a-MSH, of different concentrations of peptide extract (cellular activity and melanin production are indicated in (%), i.e. the percentage of the experimental group compared to the positive control group) (n=3, mean ± standard deviation).

[0026] (B) Reduction of the effects of tyrosinase: the peptide extract has no effect of tyrosinase decrease at a solids concentration of 0.2 ~ 0.8 mg / mL, and begins to have a tyrosinase decrease effect at around a solids concentration of 1.6 mg / mL, with the effect becoming more significant as the concentration increases. At a peptide extract solids concentration of 9.0 mg / mL (100% stock solution), the tyrosinase decrease rate reaches 55.77%.

[0027] (2) Has a restorative effect on epidermis-forming cells after an ex UVA position: Using HaCaT (human keratin-forming cell line) as an experimental model to evaluate the protective effect of HaCaT cells under UVA. The experimental results show that when the peptide extract is not added, the cellular activity of HaCaT cells after UVA exposure for 1 hour (exposure energy 20 J) is reduced by 18.6% compared with the group not exposed to UVA; when 2 mg / mL of peptide extract is added to the cell culture medium, the HaCaT cells are protected such that the cellular activity is reduced by 10.1%; when 4 mg / mL of peptide extract is added to the cell culture medium, the HaCaT cells are protected such that that cellular activity is reduced by only 7.1%, which means a protective effect of 45.7% and 62.8% respectively.

[0028] (3) Anti-aging:

[0029] (A) Promoting effect of type I procollagen production: the addition of a Peptide extract at a concentration of 2 mg / mL ~ 8 mg / mL (w / v) has a promoting effect on the formation of type I procollagen of Hs68 fibroblast. When the concentration of the added peptide extract is 8 mg / mL (w / v), there is a 56.8% increase (compared to the control group) in the formation rate of type I procollagen. The experimental results are shown in Table 2 below: Control group Experimental group Added peptide extract (mg / mL) 2 4 8 Increase in type I procollagen formation rate (%) 0.0 5.9 16.7 56.8

[0030] Table 2: Effects on the increase in the formation rate (%) of type I procollagen in Hs68 cells of different concentrations of peptide extract (compared to the control group).

[0031] (B) Decrease in decomposition by the enzyme MMP-1: photoaging of The effect of UV light on the skin is mainly due to UV light inducing active oxygen, causing an increase in the formation of, for example, Matrix Metalloproteinase-1 (MMP-1, a collagenase), which degrades collagen and elastin in the extracellular matrix, causing wrinkles or even sagging of the skin. The experimental results on the peptide extract of the present invention show that the addition of the peptide extract with a concentration above 2 mg / mL provides a decreasing effect on the MMP-1 performance of human fibroblast Hs68. When the concentration of the added peptide extract is 4.0 mg / mL (w / v), the decrease rate of MMP-1 performance is about 50% (compared with the control group). The experimental results are shown in Table 3 below: Control group Experimental group Added peptide extract (mg / mL) 2 4 8 16 Rate of decrease (%) 0.0 20.4 50.9 78.2 97.8

[0032] Table 3: Effects on the rate of decrease (%) of MMP-1 performance in Hs68 cells of different concentrations of peptide extract (compared with the control group)

[0033] In addition, the peptide extract also passed the following tests:

[0034] (1) Heavy metal test: Test carried out on the peptide extract for arsenic, lead, cadmium, mercury, etc. The results show that arsenic, lead, cadmium and mercury are not found;

[0035] (2) Total bacterial count test: Test carried out on the peptide extract using a differential medium. The results show that bacteria that should not be detected under legislation and regulations, such as Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Candida albicans, are not found;

[0036] (3) In vitro skin irritation test: Carrying out a skin irritation test on the peptide extract according to the guidelines of the OECD TG 439 skin irritation test. The result is non-irritant (NI);

[0037] (4) In vitro eye irritation test: Carrying out an eye irritation test on the peptide extract according to the guidelines of the OECD TG 492 eye irritation test. The result is non-irritant (NI);

[0038] (5) Skin sensitization test: Carrying out a skin sensitization test on the peptide extract according to the guidelines of the OECD TG 442C skin sensitization test. The result is non-sensitizing.

[0039] Therefore, the peptide extract of the present invention is safe and compliant with legislation, and has whitening, repairing and anti-aging effects.

[0040] In one embodiment, the mollusk shell is derived from mollusks having a nacre layer, including oysters, clams, baby abalone, scallops, abalone, green mussels, golden clams, or venus clams.

[0041] In one embodiment, the solvent in the solvent reservoir 120 comprises water. When mixing the mollusk shell powder and water, the volume of the mollusk shell powder is greater than that of the water, preferably with a mixing ratio of 1:0.8.

[0042] In one embodiment, the high temperature and high pressure treatment condition is a temperature between 110°C and 400°C, a pressure between 101325 Pa and 253312.5 Pa (1 and 2.5 atm), a heating time between 2 and 4 hours. Preferably, the high temperature treatment condition is a temperature between 130°C and 300°C.

[0043] Referring to [Fig.2], a functional diagram of an extraction system for a A functional peptide extract according to another embodiment of the present invention is shown. In this embodiment, the extraction system for a functional peptide extract 100' further comprises a grinding device 160 connected to the raw material tank 110, for powdering the mollusk shells and grinding the shell powder to have a particle size between 20 μm and 100 μm, and transporting the shell powder into the raw material tank 110 for subsequent mixing, extraction and filtration processes.

[0044] Referring to [Fig. 3], a block diagram of an extraction system for a functional peptide extract according to yet another embodiment of the present invention is shown. In this embodiment, the extraction system for obtaining a functional peptide extract 100" further comprises a cleaning device 170 connected to the grinding device 160, for cleaning the mollusk shells and transporting the cleaned shells into the grinding device 160 for subsequent grinding, mixing, extraction and filtration processes.

[0045] Furthermore, referring to [Fig. 4], an isometric diagram of a container for a functional peptide extract of the present invention is shown. According to the present invention, there is also provided a functional peptide extract container 200, comprising: a container body 210 having a container chamber 211, and having an opening 212 on the top; a peptide extract 220 disposed in the container chamber 211, the peptide extract 220 being extracted from the extraction system described above; and a lid 230 removably covering the opening 212 of the container body 210.

[0046] The peptide extract may be mixed with an edible substance to form a health food substance, or may be mixed with an emulsion to form a skin care product substance. The edible substance includes dairy products, mushroom extracts, alginates, antler glue, animal glue, collagen, pectin, or pure water. The emulsion includes stearic acid, propylene glycol, sorbitol, lanolin, beeswax, tocopherol, petrolatum, a surfactant, or water.

[0047] The present invention therefore provides an extraction system for a functional peptide extract and a product containing this extract. The system makes it possible to extract a functional peptide extract from mollusk shells, the peptide extract having whitening, UV repairing and anti-aging effects, which effectively transforms the waste into a functional substance with high economic value.

[0048] In conclusion, the embodiments described above are merely preferred possible embodiments, and do not limit the scope of protection of the present invention. Any equivalent structural modification of the principles which are the basis of the present description and drawings should be considered to be covered by the scope of the present invention. Description of reference figures

[0049] 100, 100', 100" Extraction system for a functional peptide extract

[0050] 110 Raw material tank

[0051] 120 Solvent tank

[0052] 130 Mixing tank

[0053] 140 High temperature and high pressure extraction device

[0054] 150 Filtration device

[0055] 160 Grinding device

[0056] 170 Cleaning device

[0057] 200 Product containing a functional peptide extract

[0058] 210 Container body

[0059] 211 Container chamber

[0060] 212 Opening

[0061] 220 Peptide extract

[0062] 230 Cover

Claims

Claims

1. An extraction system for obtaining a functional peptide extract, characterized in that it comprises: a raw material tank (110) for storing a mollusk shell powder; a solvent tank (120) for storing a solvent; a mixing tank (130) connected to both the raw material tank (110) and the solvent tank (120), the mollusk shell powder and the solvent flowing into the mixing tank (130) and being mixed therein to form a mixture; a high-temperature and high-pressure extraction device (140) connected to the mixing tank (130), the mixture flowing into the high-temperature and high-pressure extraction device (140) and being extracted therein under high-temperature and high-pressure conditions to obtain a crude extract;and a filtration device (150) connected to the high temperature and high pressure extraction device (140) for filtering the crude extract to isolate the peptide extract.;

2. An extraction system for obtaining a functional peptide extract, according to claim 1, characterized in that the mollusc shell comes from molluscs having a layer of nacre, including oysters, clams, baby abalones, scallops, abalones, green mussels, golden clams or venus clams.

3. Extraction system for obtaining a functional peptide extract, according to claim 1, characterized in that the shell powder has a particle size between 20 qm and 100 qm.

4. Extraction system for obtaining a functional peptide extract, according to claim 1, characterized in that the solvent comprises water, the shell powder and the water having a mixing ratio of 1:0.8 V / V.

5. Extraction system for obtaining a functional peptide extract, according to claim 1, characterized in that the high temperature and high pressure condition is a temperature between 110°C and 400°C, a pressure between 101325 Pa and 253312.5 Pa (1 and 2.5 atm), and a heating time between 2 and 4 hours.

6. Extraction system for obtaining a peptide extract functional, according to claim 5, characterized in that the high temperature condition is a temperature between 130°C and 300°C.

7. Extraction system for obtaining a functional peptide extract, according to claim 1, characterized in that it further comprises a grinding device (160) connected to the raw material reservoir (110) for reducing the mollusc shells to powder and transporting them into the raw material reservoir (110).

8. Extraction system for obtaining a functional peptide extract, according to claim 7, characterized in that it further comprises a cleaning device (170) connected to the grinding device (160) for cleaning the mollusc shells and transporting them into the grinding device (160).

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