Composition comprising pomegranate skin and pomegranate seed oil

Abstract

Composition comprising a mixture of pomegranate peel and pomegranate seed oil The present invention relates to a composition comprising the peel of the fruit of Punica granatum, in particulate form or in extract form, and the seed oil of the fruit of Punica granatum in a mixture, in particular for its cosmetic, therapeutic and / or nutraceutical applications. Figure for abstract: [Fig. 1]

Description

Title of the invention: Composition comprising pomegranate skin and pomegranate seed oil

[0001] The present invention relates to a composition comprising the skin of the fruit of Punica granatum, in particulate form or in extract form, and the seed oil of the fruit of Punica granatum, as a mixture, in particular for its cosmetic, therapeutic and / or nutraceutical applications.

[0002] Punica granatum is a shrub of the Lythraceae family, with deciduous leaves, which can reach up to 8 meters in height.

[0003] The fruit of Punica granatum, more commonly known as pomegranate, is a well-known fruit and has been used for centuries in traditional medicine, particularly in the Middle East and North Africa regions.

[0004] Over the last decade, evidence of the beneficial effects of pomegranate consumption on health has increased, mainly due to the increase in the number of studies on the effects of pomegranate juice on humans. Effects such as cardioprotective, neuroprotective, anticancer, antioxidant, antimicrobial and prebiotic activities have been attributed to pomegranate and its consumption. However, with regard to human health, the EFSA commission rejected all health claims related to pomegranate consumption in 2010 and 2015, particularly due to the high variability in the conclusions of the studies.

[0005] The benefits of pomegranate are mainly attributed to its high polyphenol content.

[0006] Pomegranate peel contains a large number of molecules including saponins, steroids, alkaloids, tannins, carbohydrates, flavonoids, flavonols, anthraquinones, proanthocyanidins, glycosides, reducing sugars, phenols, etc. The most abundant compounds are punicalagin and ellagic acid derivatives which are polyphenols.

[0007] The juice has a composition similar to that of the skin, but the contents of these different compounds are lower.

[0008] The seed oil contains several saturated and polyunsaturated fatty acids such as punicic acid (omega-5), linoleic acid, α-oleostearic acid, and α-talpic acid. The most abundant fatty acid is punicic acid, a long-chain omega-5 polyunsaturated fatty acid that is a positional and geometric isomer of α-linolenic acid.

[0009] The qualitative and quantitative composition of bioactives in the pomegranate fruit can vary depending on the harvesting areas, the level of maturation, the parts of the fruit (juice, skin or seeds) and the transformation process.

[0010] Application WO2006022502 discloses the use of ellagitannins for the treatment of autoimmune diseases. This application does not disclose the use of a composition comprising the skin of the fruit of Punica granatum, in particulate form or in extract form, and the seed oil of the fruit of Punica granatum as a mixture.

[0011] In addition, ellagitannins are purified and isolated from pomegranate pericarp by a process comprising numerous treatment steps with products potentially toxic to the patient being treated and / or to the environment, in particular acetone or methanol.

[0012] Application WO2005097106A1 discloses a composition comprising punic-calagin and ellagic acid extracted from pomegranate fruit skin, in particular for pharmaceutical, nutraceutical and cosmetic applications.

[0013] This application does not disclose the use of a composition comprising Punica granatum fruit skin, in particulate form or in extract form, and Punica granatum fruit seed oil as a mixture.

[0014] In addition, the punicalagin and ellagic acid of the composition according to application WO2005097106A1 are purified by a complex process on an adsorbent column.

[0015] Application WO2011038116 discloses a pharmaceutical composition comprising polyphenols obtained from pomegranate for the treatment of bone disorders. This application does not disclose the use of a composition comprising the skin of the fruit of Punica granatum, in particulate form or in extract form, and the seed oil of the fruit of Punica granatum as a mixture.

[0016] The polyphenols according to application WO2011038116 are in particular extracted from pomegranate fruit skin having undergone enzymatic treatment and purification steps on a resin column.

[0017] Particularly surprisingly, the applicant has developed a composition whose manufacturing process and finished product are safe for the patient and for the environment.

[0018] The composition developed by the applicant comprises a combination of two active ingredients derived from pomegranate having synergistic activity for the treatment of inflammatory disorders at the level of various systems, and / or osteoarticular, in particular osteoarthritis.

[0019] It is more particularly recommended for applications in the treatment of bone, cartilage, cardiovascular and / or cognitive disorders.

[0020] It is finally particularly suitable for administration to humans and animals, in particular to dogs, cats and / or horses.

[0021] The subject of the present invention is a composition comprising the skin of the fruit of Punica granatum, in particulate or extract form, and Punica granatum fruit seed oil in a mixture.

[0022] For the purposes of the present invention, the term “fruit of Punica granatum” means the fruit commonly called pomegranate.

[0023] In one embodiment, the Punica granatum is of the variety Wonderful, Molar, Bigfull, NFG-102, Emek, Shani and / or Acco.

[0024] For the purposes of the present invention, the term “skin of the fruit of Punica granatum” means the layers surrounding the arils.

[0025] In one embodiment, the skin of the Punica granatum fruit is in particulate form.

[0026] In one embodiment, the skin of the Punica granatum fruit is in particulate form with particles ranging in size from 50 pm to 1 mm.

[0027] In one embodiment, the skin of the Punica granatum fruit is in particulate form with particles ranging in size from 80 pm to 850 pm.

[0028] In one embodiment, the skin of the Punica granatum fruit is in particulate form with particles ranging in size from 100 pm to 600 pm.

[0029] In one embodiment, the skin of the Punica granatum fruit is in particulate form with particles ranging in size from 300 pm to 500 pm.

[0030] In one embodiment, the skin of the fruit of Punica granatum in particulate form is a ground skin of the fruit of Punica granatum.

[0031] In one embodiment, the skin of the Punica granatum fruit is in extract form.

[0032] In one embodiment, the Punica granatum fruit peel in extract form is an aqueous extract of Punica granatum fruit peel.

[0033] In one embodiment, the Punica granatum fruit skin extract is an aqueous extract of crushed Punica granatum fruit skin.

[0034] In one embodiment, the skin of the fruit of Punica granatum is freeze-dried.

[0035] In one embodiment, the skin of the fruit of Punica granatum in particulate form or in extract form is freeze-dried.

[0036] Freeze-drying the skin of Punica granatum fruit has the advantage of increasing the polyphenol content compared to conventional drying by eliminating at least some of the bound water. In addition, during conventional drying at least some of the polyphenols are degraded.

[0037] In one embodiment, the Punica granatum fruit skin is a ground freeze-dried Punica granatum fruit skin.

[0038] In one embodiment, the skin of the Punica granatum fruit has not undergone an extraction step on adsorbent resin.

[0039] In one embodiment, the skin of the fruit of Punica granatum in particulate form or in extract form is characterized in that it comprises at least one polyphenol.

[0040] In one embodiment, the skin of the fruit of Punica granatum in particulate form or in extract form is characterized in that it comprises at least one polyphenol chosen from the group comprising punicalagin and / or ellagic acid.

[0041] In one embodiment, the skin of the fruit of Punica granatum in particulate form or in extract form is characterized in that its mass content of polyphenols is within a range from 1% to 60% (m / m) relative to the total mass of the skin of the fruit of Punica granatum in particulate form or in extract form.

[0042] In one embodiment, the skin of the fruit of Punica granatum in particulate form or in extract form is characterized in that its mass content of polyphenols is within a range from 3% to 50% (m / m) relative to the total mass of the skin of the fruit of Punica granatum in particulate form or in extract form.

[0043] In one embodiment, the skin of the fruit of Punica granatum in particulate form or in extract form is characterized in that its mass content of polyphenols is within a range of 5% to 40% (m / m) relative to the total mass of the skin of the fruit of Punica granatum in particulate form or in extract form.

[0044] In one embodiment, the skin of the fruit of Punica granatum in particulate form or in extract form is characterized in that its mass content of polyphenols is within a range of 20% to 35% (m / m) relative to the total mass of the skin of the fruit of Punica granatum in particulate form or in extract form.

[0045] In one embodiment, the skin of the fruit of Punica granatum in particulate form or in extract form is characterized in that its mass content of punicalagin is within a range of 1% to 50% (m / m) relative to the total mass of the skin of the fruit of Punica granatum in particulate form or in extract form.

[0046] In one embodiment, the skin of the fruit of Punica granatum in particulate form or in extract form is characterized in that its mass content of punicalagin is in a range from 5% to 40% (m / m) relative to the total mass of the skin of the fruit of Punica granatum in particulate form or in extract form.

[0047] In one embodiment, the skin of the fruit of Punica granatum in particulate form or in extract form is characterized in that its mass content of punic-calagin is within a range of 15% to 45% (m / m) relative to the total mass of the skin of the fruit of Punica granatum in particulate form or in extract form.

[0048] In one embodiment, the pomegranate fruit skin in particulate form or in extract form is characterized in that its mass content of punicalagin is in the range of 20% to 30% (m / m) relative to the total mass of the pomegranate fruit skin in particulate form or in extract form.

[0049] In one embodiment, the pomegranate fruit skin in particulate form or in extract form is characterized in that its mass content of punicalagin is in the range of 0.5% to 20% (m / m) relative to the total mass of the pomegranate fruit skin in particulate form or in extract form.

[0050] In one embodiment, the skin of the fruit of Punica granatum in particulate form or in extract form is characterized in that its mass content of punic-calagin is within a range of 1% to 15% (m / m) relative to the total mass of the skin of the fruit of Punica granatum in particulate form or in extract form.

[0051] In one embodiment, the skin of the fruit of Punica granatum in particulate form or in extract form is characterized in that its mass content of punic-calagin is within a range of 3% to 10% (m / m) relative to the total mass of the skin of the fruit of Punica granatum in particulate form or in extract form.

[0052] In one embodiment, the skin of the fruit of Punica granatum in particulate form or in extract form is characterized in that its mass content of punic-calagin is within a range of 4% to 5% (m / m) relative to the total mass of the skin of the fruit of Punica granatum in particulate form or in extract form.

[0053] For the purposes of the present invention, the term “seed of the fruit of Punica granatum” means the arils of the fruit of Punica granatum freed from the flesh and juice.

[0054] In one embodiment, the seed oil of the fruit of Punica granatum is obtained by cold pressing.

[0055] In one embodiment, the seed oil of the fruit of Punica granatum is characterized in that it comprises fatty acids.

[0056] In one embodiment, the seed oil of the fruit of Punica granatum is characterized in that it comprises fatty acids selected from the group comprising punicic acid.

[0057] In one embodiment, the seed oil of the fruit of Punica granatum is characterized in that its mass content of punicic acid is comprised in a range from 40% to 95% (m / m) relative to the total mass of fatty acids of the seed oil of the fruit of Punica granatum.

[0058] In one embodiment, the seed oil of the fruit of Punica granatum is characterized in that its mass content of punicic acid is comprised in a range from 50% to 90% (m / m) relative to the total mass of fatty acids of the seed oil of the fruit of Punica granatum.

[0059] In one embodiment, the seed oil of the fruit of Punica granatum is characterized in that its mass content of punicic acid is comprised in a range of from 60% to 85% (m / m) relative to the total mass of fatty acids of the seed oil of the fruit of Punica granatum.

[0060] In one embodiment, the seed oil of the fruit of Punica granatum is characterized in that its mass content of punicic acid is comprised in a range of from 70% to 80% (m / m) relative to the total mass of fatty acids of the seed oil of the fruit of Punica granatum.

[0061] In one embodiment, the seed oil of the fruit of Punica granatum is characterized in that its mass content of punicic acid is comprised in a range from 40% to 95% (m / m) relative to the mass of the seed oil of the fruit of Punica granatum.

[0062] In one embodiment, the seed oil of the fruit of Punica granatum is characterized in that its mass content of punicic acid is comprised in a range of from 50% to 90% (m / m) relative to the mass of the seed oil of the fruit of Punica granatum.

[0063] In one embodiment, the seed oil of the fruit of Punica granatum is characterized in that its mass content of punicic acid is comprised in a range of from 60% to 85% (m / m) relative to the mass of the fruit of Punica granatum.

[0064] In one embodiment, the seed oil of the fruit of Punica granatum is characterized in that its mass content of punicic acid is comprised in a range of from 60% to 75% (m / m) relative to the mass of the seed oil of the fruit of Punica granatum.

[0065] According to the present invention, the skin of the fruit of Punica granatum, in particulate form or in extract form, and the seed oil of the fruit of Punica granatum are in a mixture. Hereinafter, the mixture of skin of the fruit of Punica granatum will be referred to as, in particulate form or in extract form, and seed oil of the fruit of Punica granatum alternatively under the name “the mixture”.

[0066] The mixture therefore consists of the skin of the fruit of Punica granatum, in particulate form or in extract form, as previously defined, and the seed oil of the fruit of Punica granatum as previously defined.

[0067] In one embodiment the seed oil of the fruit of Punica granatum is adsorbed onto a neutral silica-based substance such as mesoporous silica, to obtain an oil in powder form.

[0068] In one embodiment, the mixture is in liquid, solid or semi-solid form.

[0069] In one embodiment, the mixture in liquid form is a dispersion, an emulsion and / or a suspension.

[0070] In one embodiment, the mixture in liquid form is an emulsion.

[0071] In one embodiment, the mixture in liquid form is a water emulsion. in oil or an oil-in-water emulsion.

[0072] In one embodiment, the mixture in solid form is a powder.

[0073] In one embodiment, the mixture in solid form is a skin powder of Punica granatum fruit coated with dried Punica granatum seed oil.

[0074] In one embodiment, the mixture in solid form is a powder of Punica granatum fruit skin impregnated with Punica granatum seed oil.

[0075] In one embodiment, the mixture in semi-solid form is a suspension and / or a dispersion.

[0076] In one embodiment, the mixture is characterized in that its mass content of polyphenols is within a range from 2% to 50% (m / m) relative to the total mass of the mixture.

[0077] In one embodiment, the mixture is characterized in that its mass content of polyphenols is within a range from 10% to 40% (m / m) relative to the total mass of the mixture.

[0078] In one embodiment, the mixture is characterized in that its mass content of polyphenols is within a range from 20% to 35% (m / m) relative to the total mass of the mixture.

[0079] In one embodiment, the mixture is characterized in that its mass content of punicalagin is within a range from 1% to 70% (m / m) relative to the total mass of the mixture.

[0080] In one embodiment, the mixture is characterized in that its mass content of punicalagin is within a range from 5% to 50% (m / m) relative to the total mass of the mixture.

[0081] In one embodiment, the mixture is characterized in that its mass content in punicalagin is included in a range from 15% to 40% (m / m) relative to the total mass of the mixture.

[0082] In one embodiment, the mixture is characterized in that its mass content of punicalagin is within a range of 20% to 35% (m / m) relative to the total mass of the mixture.

[0083] In one embodiment, the mixture is characterized in that its mass content of punicalagin is within a range of 25% to 30% (m / m) relative to the total mass of the mixture.

[0084] In one embodiment, the mixture is characterized in that its mass content of ellagic acid is within a range from 0.5% to 20% (m / m) relative to the total mass of the mixture.

[0085] In one embodiment, the mixture is characterized in that its mass content of ellagic acid is within a range from 1% to 15% (m / m) relative to the total mass of the mixture.

[0086] In one embodiment, the mixture is characterized in that its mass content of ellagic acid is within a range of 3% to 10% (m / m) relative to the total mass of the mixture.

[0087] In one embodiment, the mixture is characterized in that its mass content of ellagic acid is within a range of 4% to 5% (m / m) relative to the total mass of the mixture.

[0088] In one embodiment, the mixture is characterized in that its mass content of punicic acid is within a range from 40% to 95% (m / m) relative to the total mass of fatty acids in the mixture.

[0089] In one embodiment, the mixture is characterized in that its mass content of punicic acid is within a range from 50% to 90% (m / m) relative to the total mass of fatty acids in the mixture.

[0090] In one embodiment, the mixture is characterized in that its mass content of punicic acid is within a range of 60% to 85% (m / m) relative to the total mass of fatty acids in the mixture.

[0091] In one embodiment, the mixture is characterized in that its mass content of punicic acid is within a range of 70% to 80% (m / m) relative to the total mass of fatty acids in the mixture.

[0092] In one embodiment, the mixture is characterized in that the ratio between the mass content of skin of the fruit of Punica granatum, in particulate form or in extract form, and the mass content of seed oil of the fruit of Punica granatum is within a range from 0.001 / 1 to 1000 / 1.

[0093] In one embodiment, the mixture is characterized in that the ratio between the mass content of skin of the fruit of Punica granatum, in particulate form or in extract form, and the mass content of seed oil from the fruit of Punica granatum is in the range of 0.01 / 1 to 100 / 1.

[0094] In one embodiment, the mixture is characterized in that the ratio between the mass content of skin of the fruit of Punica granatum, in particulate form or in extract form, and the mass content of seed oil of the fruit of Punica granatum is within a range from 1 / 1 to 50 / 1.

[0095] In one embodiment, the mixture is characterized in that the ratio between the mass content of skin of the fruit of Punica granatum, in particulate form or in extract form, and the mass content of seed oil of the fruit of Punica granatum is within a range from 5 / 1 to 20 / 1.

[0096] In one embodiment, the mixture is characterized in that the ratio between the mass content of skin of the fruit of Punica granatum, in particulate form or in extract form, and the mass content of seed oil of the fruit of Punica granatum is within a range from 7 / 1 to 15 / 1.

[0097] In one embodiment, the mixture is characterized in that the ratio between the mass content of skin of the fruit of Punica granatum, in particulate form or in extract form, and the mass content of seed oil of the fruit of Punica granatum is within a range from 9 / 1 to 12 / 1.

[0098] In one embodiment, the composition according to the present invention comprises a ratio between the mass content of skin of the fruit of Punica granatum, in particulate form or in extract form, and the mass content of seed oil of the fruit of Punica granatum is comprised in a range from 1 / 1 to 1 / 50.

[0099] In one embodiment, the mixture is characterized in that the ratio between the mass content of skin of the fruit of Punica granatum, in particulate form or in extract form, and the mass content of seed oil of the fruit of Punica granatum is within a range from 1 / 5 to 1 / 20.

[0100] In one embodiment, the mixture is characterized in that the ratio between the mass content of skin of the fruit of Punica granatum, in particulate form or in extract form, and the mass content of seed oil of the fruit of Punica granatum is within a range from 1 / 7 to 1 / 15.

[0101] In one embodiment, the mixture comprises from 0.01 mg to 1 g of Punica granatum fruit skin, in particulate form or in extract form.

[0102] In one embodiment, the mixture comprises from 0.1 mg to 100 mg of Punica granatum fruit skin, in particulate form or in extract form.

[0103] In one embodiment, the mixture comprises from 0.5 mg to 50 mg of Punica granatum fruit skin, in particulate form or in extract form.

[0104] In one embodiment, the mixture comprises from 1 mg to 30 mg of Punica granatum fruit skin, in particulate form or in extract form.

[0105] In one embodiment, the mixture comprises from 0.01 ml to 1000 ml of aqueous extract of crushed Punica granatum fruit skin.

[0106] In one embodiment, the mixture comprises from 0.1 ml to 100 ml of aqueous extract of crushed Punica granatum fruit skin.

[0107] In one embodiment, the mixture comprises from 1 ml to 50 ml of aqueous extract of crushed Punica granatum fruit skin.

[0108] In one embodiment, the mixture comprises from 0.001 mg to 1 g of Punica granatum fruit seed oil.

[0109] In one embodiment, the mixture comprises from 0.005 mg to 10 mg of Punica granatum fruit seed oil.

[0110] In one embodiment, the mixture comprises from 0.01 mg to 2 mg of Punica granatum fruit seed oil.

[0111] In one embodiment, the mixture comprises from 0.001 μl to 1 ml of Punica granatum fruit seed oil.

[0112] In one embodiment, the mixture comprises from 0.1 μl to 500 μl of Punica granatum fruit seed oil.

[0113] In one embodiment, the mixture comprises from 10 μl to 400 μl of Punica granatum fruit seed oil.

[0114] In one embodiment, the mixture comprises from 50 μl to 200 μl of Punica granatum fruit seed oil.

[0115] In one embodiment, the composition according to the present invention is a cosmetic, nutraceutical and / or pharmaceutical composition and / or a food ingredient and / or a food supplement.

[0116] In one embodiment, the composition according to the present invention is a cosmetic composition.

[0117] In one embodiment, the composition according to the present invention is a nutraceutical composition.

[0118] In one embodiment, the composition according to the present invention is a pharmaceutical composition.

[0119] In one embodiment, the composition according to the present invention is a food ingredient and / or a food supplement.

[0120] In one embodiment, the composition according to the present invention is a cosmetic, nutraceutical and / or pharmaceutical active ingredient.

[0121] In one embodiment, the composition according to the present invention is a cosmetic active ingredient.

[0122] In one embodiment, the composition according to the present invention is a nutraceutical active ingredient.

[0123] In one embodiment, the composition according to the present invention is an active pharmaceutical.

[0124] In one embodiment, the composition is in liquid, solid or semi-solid form.

[0125] In one embodiment, the composition in liquid form is a concentrate, a dispersion, drops, an emulsion and / or a suspension.

[0126] In one embodiment, the composition in liquid form is an emulsion.

[0127] In one embodiment, the composition in liquid form is a water-based emulsion. in oil or an oil-in-water emulsion.

[0128] In one embodiment, the solid form composition is a block, a cachet, a capsule, a film, granules, a chewing gum, an implant, an impregnated material, an insert, a lozenge, a lyophilizate, a patch, pellets, a pessary, pills, a plaster, a pouch, a powder, a stick, a suppository, a tablet and / or a powder of Punica granatum fruit skin coated with dried Punica granatum seed oil.

[0129] In one embodiment, the composition in solid form is a powder of Punica granatum fruit skin coated with dry Punica granatum seed oil.

[0130] In one embodiment, the composition in semi-solid form is a cream, a foam, a gel, an ointment, a paste and / or a poultice.

[0131] In one embodiment, the composition in semi-solid form is poured into a liquid specialty delivery device comprising a soft gelatin reservoir and a cap hermetically closing the reservoir, further comprising a tab disposed on the opposite side of the cap relative to the reservoir, the tab being filled with soft gelatin as described in application WO2021156557 (Al).

[0132] In one embodiment, the composition according to the present invention is characterized in that its mass content of mixture is within a range from 1% to 100% (m / m) relative to the total mass of the composition.

[0133] In one embodiment, the composition according to the present invention is characterized in that its mass content of mixture is within a range from 5% to 80% (m / m) relative to the total mass of the composition.

[0134] In one embodiment, the composition according to the present invention is characterized in that its mass content of mixture is within a range from 10% to 70% (m / m) relative to the total mass of the composition.

[0135] In one embodiment, the composition according to the present invention consists of the mixture of skin of the fruit of Punica granatum, in particulate form or in extract form, and seed oil of the fruit of Punica granatum relative to the total mass of the composition.

[0136] In one embodiment, the composition according to the present invention is characterized in that its mass content of polyphenol is within a range ranging from 1% to 60% (m / m) relative to the total mass of the composition according to the present invention.

[0137] In one embodiment, the composition according to the present invention is characterized in that its mass content of polyphenol is within a range from 2% to 50% (m / m) relative to the total mass of the composition according to the present invention.

[0138] In one embodiment, the composition according to the present invention is characterized in that its mass content of polyphenol is within a range from 10% to 40% (m / m) relative to the total mass of the composition according to the present invention.

[0139] In one embodiment, the composition according to the present invention is characterized in that its mass content of polyphenol is within a range from 20% to 35% (m / m) relative to the total mass of the composition according to the present invention.

[0140] In one embodiment, the composition according to the present invention is characterized in that its mass content of punicalagin is within a range from 1% to 70% (m / m) relative to the total mass of the composition according to the present invention.

[0141] In one embodiment, the composition according to the present invention is characterized in that its mass content of punicalagin is within a range from 5% to 50% (m / m) relative to the total mass of the composition according to the present invention.

[0142] In one embodiment, the composition according to the present invention is characterized in that its mass content of punicalagin is within a range from 15% to 40% (m / m) relative to the total mass of the composition according to the present invention.

[0143] In one embodiment, the composition according to the present invention is characterized in that its mass content of punicalagin is within a range from 20% to 35% (m / m) relative to the total mass of the composition according to the present invention.

[0144] In one embodiment, the composition according to the present invention is characterized in that its mass content of punicalagin is within a range from 25% to 30% (m / m) relative to the total mass of the composition according to the present invention.

[0145] In one embodiment, the composition according to the present invention is characterized in that its mass content of ellagic acid is within a range from 0.5% to 20% (m / m) relative to the total mass of the composition according to the present invention.

[0146] In one embodiment, the composition according to the present invention is characterized in that its mass content of ellagic acid is within a range from 1% to 15% (m / m) relative to the total mass of the composition according to the present invention.

[0147] In one embodiment, the composition according to the present invention is characterized in that its mass content of ellagic acid is within a range from 3% to 10% (m / m) relative to the total mass of the composition according to the present invention.

[0148] In one embodiment, the composition according to the present invention is characterized in that its mass content of ellagic acid is within a range from 4% to 5% (m / m) relative to the total mass of the composition according to the present invention.

[0149] In one embodiment, the composition according to the present invention is characterized in that its mass content of punicic acid is comprised in a range from 40% to 95% (m / m) relative to the total mass of fatty acids of the composition according to the present invention.

[0150] In one embodiment, the composition according to the present invention is characterized in that its mass content of punicic acid is within a range from 50% to 90% (m / m) relative to the total mass of fatty acids of the composition according to the present invention.

[0151] In one embodiment, the composition according to the present invention is characterized in that its mass content of punicic acid is within a range from 60% to 85% (m / m) relative to the total mass of fatty acids of the composition according to the present invention.

[0152] In one embodiment, the composition according to the present invention is characterized in that its mass content of punicic acid is in the range from 70% to 80% (m / m) relative to the total mass of the fatty acids of the composition according to the present invention.

[0153] In one embodiment, the composition according to the present invention does not comprise pomegranate juice.

[0154] In one embodiment, the composition according to the present invention does not comprise pomegranate fruit flesh.

[0155] In one embodiment, the composition according to the present invention does not comprise pomegranate juice and / or does not comprise pomegranate fruit flesh.

[0156] In one embodiment, the composition according to the present invention does not comprise pomegranate juice nor pomegranate fruit flesh.

[0157] In one embodiment, the composition according to the present invention does not comprise no urolithin or isourolithin, in particular no urolithin A, urolithin B, urolithin C, urolithin D, urolithin E, urolithin M3, urolithin M4, urolithin M5, urolithin M6, urolithin M7 and isourolithin A and / or isourolithin B.

[0158] In one embodiment, the composition according to the present invention does not comprise Punica granatum juice and / or Punica granatum fruit flesh, urolithin or isourolithin, in particular no urolithin A, urolithin B, urolithin C, urolithin D, urolithin E, urolithin M3, urolithin M4, urolithin M5, urolithin M6, urolithin M7 and isourolithin A and / or isourolithin B.

[0159] In one embodiment, the composition according to the invention is characterized in that the ratio between the mass content of skin of the fruit of Punica granatum, in particulate form or in extract form, and the mass content of seed oil of the fruit of Punica granatum is within a range from 0.001 / 1 to 1000 / 1.

[0160] In one embodiment, the composition according to the invention is characterized in that the ratio between the mass content of skin of the fruit of Punica granatum, in particulate form or in extract form, and the mass content of seed oil of the fruit of Punica granatum is within a range from 0.01 / 1 to 100 / 1.

[0161] In one embodiment, the composition according to the invention is characterized in that the ratio between the mass content of skin of the fruit of Punica granatum, in particulate form or in extract form, and the mass content of seed oil of the fruit of Punica granatum is within a range from 1 / 1 to 50 / 1.

[0162] In one embodiment, the composition according to the invention is characterized in that the ratio between the mass content of skin of the fruit of Punica granatum, in particulate form or in extract form, and the mass content of seed oil of the fruit of Punica granatum is within a range from 5 / 1 to 20 / 1.

[0163] In one embodiment, the composition according to the invention is characterized in that the ratio between the mass content of skin of the fruit of Punica granatum, in particulate form or in extract form, and the mass content of seed oil of the fruit of Punica granatum is within a range from 7 / 1 to 15 / 1.

[0164] In one embodiment, the composition according to the invention is characterized in that the ratio between the mass content of skin of the fruit of Punica granatum, in particulate form or in extract form, and the mass content of seed oil of the fruit of Punica granatum is within a range from 9 / 1 to 12 / 1.

[0165] In one embodiment, the composition according to the present invention comprises a ratio between the mass content of skin of the fruit of Punica granatum, in particulate form or in extract form, and the mass content of seed oil of the fruit of Punica granatum is comprised in a range from 1 / 1 to 1 / 50.

[0166] In one embodiment, the composition according to the invention is characterized in that that the ratio between the mass content of skin of the fruit of Punica granatum, in particulate form or in extract form, and the mass content of seed oil of the fruit of Punica granatum is within a range from 1 / 5 to 1 / 20.

[0167] In one embodiment, the composition according to the invention is characterized in that the ratio between the mass content of skin of the fruit of Punica granatum, in particulate form or in extract form, and the mass content of seed oil of the fruit of Punica granatum is within a range from 1 / 7 to 1 / 15.

[0168] In one embodiment, the composition according to the invention comprises from 0.01 mg to 1 g of skin of the fruit of Punica granatum, in particulate form or in extract form.

[0169] In one embodiment, the composition according to the invention comprises from 0.1 mg to 100 mg of skin of the fruit of Punica granatum, in particulate form or in extract form.

[0170] In one embodiment, the composition according to the invention comprises from 0.5 mg to 50 mg of skin of the fruit of Punica granatum, in particulate form or in extract form.

[0171] In one embodiment, the composition according to the invention comprises from 1 mg to 30 mg of skin of the fruit of Punica granatum, in particulate form or in extract form.

[0172] In one embodiment, the composition according to the invention comprises from 0.001 mg to 1 g of seed oil from the fruit of Punica granatum.

[0173] In one embodiment, the composition according to the invention comprises from 0.005 mg to 10 mg of seed oil from the fruit of Punica granatum.

[0174] In one embodiment, the composition according to the invention comprises from 0.01 mg to 2 mg of seed oil from the fruit of Punica granatum.

[0175] In one embodiment, the composition according to the present invention further comprises at least one pharmaceutically and / or cosmetically acceptable excipient.

[0176] In one embodiment, the composition according to the present invention further comprises at least one pharmaceutically and / or cosmetically acceptable excipient chosen from the list presented in Regulation (EU) No. 1129 / 2011 of the European Commission of November 11, 2011.

[0177] In one embodiment, the composition according to the present invention further comprises at least one pharmaceutically and / or cosmetically acceptable excipient chosen from the list presented in Directive 2002 / 46 / EC of the European Parliament and of the Council of June 10, 2002.

[0178] In one embodiment, the composition according to the present invention further comprises at least one pharmaceutically and / or cosmetically acceptable excipient chosen from the group comprising: surfactants, polymers, copolymers, water-soluble polymers, humectants, fatty / oily components, hardened oils, synthetic triglycerides and diglycerides, waxes, hydrocarbons, higher fatty acids, higher alcohols, esters, essential oils, silicone oils, inorganic salts, inorganic acids, colorants, binders, disintegrants, diluents, flavoring agents, fillers, lubricants, wetting agents, suspending agents, emulsifying agents, antimicrobial and / or antioxidant preservatives, sweeteners, prebiotics, probiotics, pomegranate seed oil cake, chelating and / or complexing agents, glazing agents, emollients, glidants, plasticizers, aqueous solvents, veterinary palatants and / or buffer salts.

[0179] In one embodiment, the composition according to the present invention further comprises at least one chelating and / or complexing agent.

[0180] In one embodiment, the composition according to the present invention further comprises at least one chelating and / or complexing agent chosen from the group comprising cyclodextrin, citric acid and / or malic acid.

[0181] In one embodiment, the composition according to the present invention further comprises at least one emollient.

[0182] In one embodiment, the composition according to the present invention further comprises at least one emollient chosen from the group comprising vegetable oils, mineral oils, xylitol and / or propylene glycol.

[0183] In one embodiment, the composition according to the present invention further comprises at least one slip agent.

[0184] In one embodiment, the composition according to the present invention further comprises at least one slip agent chosen from the group comprising colloidal silica.

[0185] In one embodiment, the composition according to the present invention further comprises at least one coating agent.

[0186] In one embodiment, the composition according to the present invention further comprises at least one coating agent chosen from the group comprising cellulose derivatives, polyvinyls, starch.

[0187] In one embodiment, the composition according to the present invention further comprises at least one plasticizer.

[0188] In one embodiment, the composition according to the present invention further comprises at least one plasticizer chosen from the group comprising acetyltributyl citrate, glycerin, mannitol and / or sorbitol.

[0189] In one embodiment, the composition according to the present invention further comprises at least one surfactant.

[0190] For the purposes of the present invention, the term “surfactant” means a compound which modifies the surface tension between two surfaces, phases. Generally speaking, these compounds are amphiphilic molecules and allow two immiscible phases of a composition to be solubilized.

[0191] In one embodiment, the surfactant is selected from the group comprising anionic surfactants, cationic surfactants, nonionic surfactants, and / or silicone-based surfactants and / or fluorine-based surfactants.

[0192] In one embodiment, the anionic surfactants are selected from the group consisting of fatty acid soaps, sulfonate anionic surfactants, sulfate anionic surfactants, phosphate anionic surfactants, acylmethyltaurine salts, monoalkyl phosphates, acylglutamates, and / or isethionic acid ester salts.

[0193] In one embodiment, the cationic surfactants are selected from the group comprising cationic surfactants of amine salt type and / or cationic surfactants of quaternary ammonium type (tetraalkylammonium type and pyridinium type).

[0194] In one embodiment, the non-ionic surfactants are selected from the group comprising glycerin fatty acid esters, propylene glycol fatty acid esters, sorbitan fatty acid esters, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene tetraoleic acid sorbitol, polyoxyethylene alkyl ethers, polyoxyethylene polyoxypropylene glycols, polyoxyethylene polyoxypropylene alkyl ethers, polyethylene glycol fatty acid esters, polyoxyethylenated castor oils, hydrogenated polyoxyethylenated castor oils, and / or polyglycerol fatty acid esters.

[0195] In one embodiment, the amphoteric surfactants are selected from the group comprising imidazoline-type surfactants, betaine-type surfactants and / or amino acid-type surfactants.

[0196] In one embodiment, the composition according to the present invention further comprises at least one water-soluble polymer.

[0197] In one embodiment, the water-soluble polymer is selected from the group consisting of sodium alginate, propylene glycol esters of alginic acid, gum arabic, xanthan gum, pectin, tragacanth, sodium carboxymethylcellulose, methylcellulose, carboxyvinyl polymers, polyethylene glycol, polyvinyl alcohol, polyvinylpyrrolidone, cationized cellulose, cationized dextran, cationized dextrin, chitosan, cationized vinylpyrrolidone polymers, N,N-dimethyl-3,5-methylenepiperidinium chloride polymers, milk protein, soy protein, gelatin, egg protein, sodium casein and / or whey protein.

[0198] In one embodiment, the composition according to the present invention comprises in besides at least one humectant.

[0199] In one embodiment, the humectant is selected from the group consisting of polyols, amino acids, mucopolysaccharides, proteins, biological extracts, fermentation metabolites, polysaccharides, plant extracts, phospholipids and / or ceramides.

[0200] In one embodiment, the composition according to the present invention further comprises at least one emollient.

[0201] In one embodiment, the emollient is selected from the group consisting of fats and oils, including soybean oil, bran oil, jojoba oil, avocado oil, almond oil, cocoa oil, olive oil, sesame oil, Persian oil, castor oil and / or palm oil, corn oil, mineral oils, mink oil, beef tallow and / or lard.

[0202] In one embodiment, the composition according to the present invention further comprises at least one synthetic triglyceride and / or diglyceride.

[0203] In one embodiment, the synthetic triglycerides and diglycerides are selected from the group comprising myristic acid glyceride and / or 2-ethylhexanoic acid glyceride.

[0204] In one embodiment, the composition according to the present invention further comprises at least one wax.

[0205] In one embodiment, the wax is selected from the group comprising camauba wax, spermaceti, beeswax and / or lanolin.

[0206] In one embodiment, the composition according to the present invention further comprises at least one hydrocarbon.

[0207] In one embodiment, the hydrocarbon is selected from the group comprising paraffin, petrolatum, microcrystalline wax, ceresin, squalane and / or pristane.

[0208] In one embodiment, the composition according to the present invention further comprises at least one higher fatty acid.

[0209] In one embodiment, the higher fatty acid is selected from the group consisting of lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, linoleic acid and / or isostearic acid.

[0210] In one embodiment, the higher alcohols are selected from the group consisting of lauryl alcohol, cetyl alcohol, stearyl alcohol, oleyl alcohol, cholesterol and / or 2-hexyldecanol.

[0211] In one embodiment, the composition according to the present invention further comprises at least one ester.

[0212] In one embodiment, the ester is selected from the group consisting of cetyl octanoate, myristyl lactate, cetyl lactate, isopropyl myristate, myristyl myristate, isopropyl palmitate, isopropyl adipate, stearate butyl, decyl oleate and / or cholesterol isostearate.

[0213] In one embodiment, the composition according to the present invention further comprises at least one essential oil.

[0214] In one embodiment, the essential oil is selected from the group consisting of mentha oil, jasmine oil, camphor oil, Japanese cypress oil, bitter orange oil, ryu oil, turpentine oil, cinnamon oil, bergamot oil, mandarin oil, calamus oil, pine oil, lavender oil, bay oil, clove oil, hiba oil, rose oil, eucalyptus oil, lemon oil, peppermint oil, thyme oil, rose oil, sage oil, menthol, cineole, eugenol, citral, citronellal, borneol, linalool, geraniol, camphor, thymol, spilanthol, pinene, limonene and / or terpenoid compounds.

[0215] In one embodiment, the composition according to the present invention further comprises at least one inorganic salt.

[0216] In one embodiment, the inorganic salts are selected from the group consisting of sodium carbonate, sodium hydrogen carbonate, sodium sesquicarbonate, borax, sodium sulfate, sodium sulfide, sodium nitrate, sodium thiosulfate, sodium polyphosphate, sodium phosphate, potassium chloride, potassium sulfide, calcium oxide, magnesium oxide, calcium carbonate and / or magnesium carbonate.

[0217] In one embodiment, the composition according to the present invention further comprises at least one inorganic acid.

[0218] In one embodiment, the inorganic acids are selected from the group comprising boric acid, metasilicic acid and / or silicic anhydride.

[0219] In one embodiment, the colorants are selected from the group consisting of yellow No. 4, blue No. 1, yellow No. 202, chlorophyll, riboflavin, safflower, crocin and / or anthraquinone.

[0220] In one embodiment, the composition according to the present invention further comprises at least one polymer.

[0221] In one embodiment, the polymers are selected from the group consisting of acrylic resins, styrene resins, epoxy resins, nylon, polyethylene, polypropylene, polyvinyl chloride, polyethylene terephthalate resins and polytetrafluoroethane, and / or copolymers of these polymers.

[0222] In one embodiment, the composition according to the present invention further comprises at least one binder.

[0223] In one embodiment, the binders are selected from the group consisting of crystalline cellulose, cellulose derivatives, gum arabic, corn starch, pregelatinized starch, polyvinylpyrrolidone, hydroxypropylmethylcellulose or gelatins.

[0224] In one embodiment, the composition according to the present invention further comprises at least one disintegrant.

[0225] In one embodiment, the disintegrants are selected from the group consisting of corn starch, potato starch, sodium carboxymethylcellulose, alginic acid, L-HPC and / or sodium starch glycolate.

[0226] In one embodiment, the composition according to the present invention further comprises at least one filler.

[0227] In one embodiment, the fillers are selected from the group comprising carbohydrates, in particular lactose, microcrystalline cellulose and / or calcium hydrogen phosphate.

[0228] In one embodiment, the composition according to the present invention further comprises at least one lubricant.

[0229] In one embodiment, the lubricants are selected from the group comprising magnesium stearate, talc and / or silica.

[0230] In one embodiment, the composition according to the present invention further comprises at least one lubricant.

[0231] In one embodiment, the wetting agents are selected from the group comprising sodium lauryl sulfate.

[0232] In one embodiment, the composition according to the present invention further comprises at least one suspending agent.

[0233] In one embodiment, the suspending agents are selected from the group consisting of sorbitol syrup, cellulose derivatives, animal and / or vegetable gelatin, agar, guar gum, gum arabic, xanthan gum, pectin, carregeenan, mannitol syrup and / or hydrogenated edible fats.

[0234] In one embodiment, the composition according to the present invention further comprises at least one emulsifying agent.

[0235] In one embodiment, the emulsifying agents are selected from the group comprising lecithin and / or acacia.

[0236] In one embodiment, the composition according to the present invention further comprises at least one preservative agent.

[0237] In one embodiment, the preservatives are selected from the group comprising methyl or propyl p-hydroxybenzoates, ascorbic acid, citric acid, malic acid, vitamin E and / or sorbic acid.

[0238] In one embodiment, the composition according to the present invention further comprises at least one veterinary appetizer.

[0239] In one embodiment, the composition according to the present invention further comprises at least one veterinary appetizer chosen from the group comprising yeasts, pork liver and / or poultry liver.

[0240] In one embodiment, the composition according to the present invention further comprises at least one veterinary appetizer chosen from the group comprising yeasts, in particular in the form of flakes.

[0241] In one embodiment, the composition according to the present invention further comprises at least one pharmaceutical active ingredient, especially a veterinary one.

[0242] In one embodiment, the composition according to the present invention further comprises at least one pharmaceutical active ingredient for the treatment and / or prevention of joint, bone, cartilage, inflammatory, cardiovascular and / or cognitive disorders.

[0243] In one embodiment, the composition according to the present invention further comprises pomegranate seed oil cakes, vitamin A, D and / or E and / or yeast extracts.

[0244] In one embodiment, the composition according to the present invention further comprises at least one pharmaceutical active ingredient for the treatment and / or prevention of atopic dermatitis, osteoarthritis, rheumatoid polyarthritis and / or osteoporosis and / or for maintaining and / or improving bone remodeling.

[0245] In one embodiment, the composition according to the present invention further comprises at least one cosmetic active ingredient.

[0246] The invention also relates to the cosmetic use of the composition according to the present invention for maintaining and / or improving the shine, hydration and / or resistance of the integuments.

[0247] In one embodiment, the appendages are chosen from the group comprising hairs and / or hair.

[0248] In one embodiment, the appendages are chosen from the group comprising hairs.

[0249] The invention also relates to the composition according to the present invention for its therapeutic and / or nutraceutical use.

[0250] For the purposes of the present invention, the term "nutraceutical" means the use of a food or a food supplement to prevent and / or treat human and / or animal diseases, in particular to restore, correct and / or modify their physiological functions by exerting a pharmacological, immunological or metabolic action.

[0251] An embodiment also relates to the composition according to the present invention for its therapeutic and / or nutraceutical use according to the present invention in which the composition is administered to the mammal.

[0252] An embodiment also relates to the composition according to the present invention for its therapeutic and / or nutraceutical use according to the present invention in which the composition is administered to humans.

[0253] An embodiment also relates to the composition according to the present invention for its therapeutic and / or nutraceutical use according to the present invention in which the composition is administered to animals.

[0254] An embodiment also relates to the composition according to the present invention for its therapeutic and / or nutraceutical use according to the present invention in which the composition is administered to the animal chosen from the group comprising the dog, the cat and / or the horse.

[0255] An embodiment also relates to the composition according to the present invention for its therapeutic and / or nutraceutical use according to the present invention in which the composition is administered to dogs.

[0256] An embodiment also relates to the composition according to the present invention for its therapeutic and / or nutraceutical use according to the present invention in which the composition is administered to cats.

[0257] An embodiment also relates to the composition according to the present invention for its therapeutic and / or nutraceutical use according to the present invention in which the composition is administered to the horse.

[0258] An embodiment also relates to the composition according to the present invention for its therapeutic and / or nutraceutical use according to the present invention for the treatment and / or prevention of joint, bone, cartilage, inflammatory, cardiovascular and / or cognitive disorders.

[0259] One embodiment also relates to the composition according to the present invention for its therapeutic and / or nutraceutical use according to the present invention for the treatment and / or prevention of atopic dermatitis, osteoarthritis, rheumatoid polyarthritis and / or osteoporosis and / or for maintaining and / or improving bone remodeling.

[0260] One embodiment also relates to the composition according to the present invention for its therapeutic and / or nutraceutical use according to the present invention in which it is administered orally, parenterally, topically, rectally, auricularly, nasally and / or ophthalmically.

[0261] One embodiment also relates to the composition according to the present invention for its therapeutic and / or nutraceutical use according to the present invention in which it is administered orally.

[0262] An embodiment also relates to the composition according to the present invention for its therapeutic and / or nutraceutical use according to the present invention in which the composition is administered in an amount allowing the administration of a dose of skin of the fruit of Punica granatum per unit of body weight, in particulate form or in extract form, included in a range ranging from 50 pg / kg to 100 mg / kg.

[0263] An embodiment also relates to the composition according to the present invention for its therapeutic and / or nutraceutical use according to the present invention in which the composition is administered in an amount allowing the administration of a dose of skin of the fruit of Punica granatum per unit of body weight, in particulate form or in extract form, comprised in a range from 100 pg / kg to 10 mg / kg.

[0264] An embodiment also relates to the composition according to the present invention for its therapeutic and / or nutraceutical use according to the present invention in which the composition is administered in an amount allowing the administration of a dose of skin of the fruit of Punica granatum per unit of body weight, in particulate form or in extract form, comprised in a range from 500 pg / kg to 1 mg / kg.

[0265] An embodiment also relates to the composition according to the present invention for its therapeutic and / or nutraceutical use according to the present invention in which the composition is administered in an amount allowing the administration of a dose of skin of the fruit of Punica granatum per unit of body weight, in particulate form or in extract form, comprised in a range from 800 pg / kg to 900 pg / kg.

[0266] An embodiment also relates to the composition according to the present invention for its therapeutic and / or nutraceutical use according to the present invention in which the composition is administered in an amount allowing the administration of a dose of skin of the fruit of Punica granatum per unit of body weight, in particulate form or in extract form, of 863 pg / kg.

[0267] An embodiment also relates to the composition according to the present invention for its therapeutic and / or nutraceutical use according to the present invention in which the composition is administered in an amount allowing the administration of a dose of seed oil of the fruit of Punica granatum per unit of body weight comprised in a range from 1 pg / kg to 1 mg / kg.

[0268] An embodiment also relates to the composition according to the present invention for its therapeutic and / or nutraceutical use according to the present invention in which the composition is administered in an amount allowing the administration of a dose of seed oil of the fruit of Punica granatum comprised in a range from 10 pg / kg to 500 pg / kg.

[0269] An embodiment also relates to the composition according to the present invention for its therapeutic and / or nutraceutical use according to the present invention in which the composition is administered in an amount allowing the administration of a dose of seed oil of the fruit of Punica granatum per unit of body weight within a range of 50 pg / kg to 100 pg / kg.

[0270] An embodiment also relates to the composition according to the present invention for its therapeutic and / or nutraceutical use according to the present invention in which the composition is administered in an amount allowing the administration of a dose of seed oil of the fruit of Punica granatum per unit of body weight of 61 pg / kg.

[0271] The present invention also relates to an administration device comprising the composition according to the present invention.

[0272] In one embodiment, the delivery device is a veterinary delivery device.

[0273] In one embodiment, the delivery device is a metering pump, a pipette, a syringe, a spray and / or a patch.

[0274] The present invention also relates to a kit comprising on the one hand an extract of the skin of the fruit of Punica granatum, in particulate form or in extract form, as defined above and on the other hand seed oil of the fruit of Punica granatum as defined above.

[0275] [Fig.l] is a photograph of the results of the BSA denaturation tests as presented in Example 3, each tube corresponding respectively from left to right to Sol. 1, Sol. 2, Sol. 3, Sol. 4.

[0276] [Fig.2] is the graphical representation of the results of the absorbance measurements measured at 660 nm of the BSA denaturation tests as presented in example 3, with on the ordinate the absorbance value measured at 660 nm and on the abscissa, respectively from left to right, Sol. 1, Sol. 2, Sol. 3, Sol. 4.

[0277] In the examples, all percentages are given by volume unless otherwise indicated, temperature is expressed in degrees Celsius unless otherwise indicated, and pressure is atmospheric pressure unless otherwise indicated.

[0278] EXAMPLES

[0279] Example 1: Process for preparing crushed skin from the fruit of Punica granatum.

[0280] Example 1.1: Process for preparing ground freeze-dried Punica granatum fruit skin.

[0281] The fruits of Punica granatum (Emec, Shani and Acco varieties, origin: France) are disinfected by immersion in acetic acid. They are then peeled manually so as to retain only the skin.

[0282] A quantity of 600 grams of skins obtained is transferred into a freeze-dryer (MARTIN CHRIST - Alpha 1-2 Plus), where a freezing step followed by a freeze-drying step are carried out according to the following methods:

[0283] The first freezing step is carried out at a temperature of -20°C;

[0284] A primary freeze-drying step is carried out by applying a vacuum at approximately 60 micro-bars for 48 hours.

[0285] The freeze-dried fruit skins are ground using a grinder (MILIAN, Pul-verisette 11) for 2 to 10 min at a speed of 2000 to 3000 rpm.

[0286] Freeze-dried and crushed fruit skin is obtained.

[0287] A portion of the freeze-dried and crushed fruit skins is stored.

[0288] The total polyphenol content is evaluated by UV-VIS assay and according to the method described in the article Georgé S, et al. J Agric Food Chem. 2005 Mar 9;53(5): 1370-3.

[0289] The content of punicalagin and ellagic acid is evaluated by HPLC according to the method described in the article Calm-Sânchez, A., et al. Food Bioprocess Technol 6, 1644-1654 (2013) for ellagic acid and punicalagin

[0290] The results are presented in Table 1. [Tables 1] Active substance Mass percentage (m / m) per 100 grams of freeze-dried and ground fruit peel is obtained according to example 1 Ellagic acid 4.544 Punicalagin 28.320 Total polyphenols 33.23

[0291] Example 1.2: Process for preparing the aqueous extract of crushed skin of the fruit of Punica granatum.

[0292] A quantity of 5 g of freeze-dried skin ground material as obtained in Example 1.1 is introduced into an Erlenmeyer flask. Purified hot water is poured onto the powder to reach a final volume of 200 ml, and the suspension obtained is mixed using a spatula about ten times.

[0293] The mixture obtained is left to cool until it reaches room temperature, then the Erlenmeyer flask is closed and left to stand for 12 hours at a temperature of 4°C.

[0294] The supernatant is filtered using a syringe filter and the aqueous extract of freeze-dried and crushed fruit peel is stored in a sealed glass bottle at 4°C.

[0295] Example 2: Process for preparing the composition according to the present invention.

[0296] Example 2.1: Process for preparing the composition according to the present invention comprising a mixture in the form of an oil-in-water emulsion.

[0297] Punica granatum fruit seed oil (Origin: France) in the form of oil obtained by separating the seeds from the surrounding arils, drying and cold pressing the seeds, is purchased commercially (Pomegranate seed oil - L'Atelier d'Hippolyte). Punica granatum fruit seed oil has a punicic acid content of approximately 70 g per 100 g of Punica fruit seed oil granatum.

[0298] A quantity of 200 ql of Punica granatum fruit seed oil is mixed in a high-shear mixer with 2ml of aqueous extract of Punica granatum fruit peel as obtained according to Example 1.2. A 2% quantity of surfactant is added.

[0299] A composition according to the invention comprising a mixture in the form of an oil-in-water emulsion is obtained.

[0300] Example 2.2: Process for preparing the composition according to the present invention comprising a mixture in the form of powder of Punica granatum fruit skin impregnated with Punica granatum seed oil.

[0301] A quantity of 100 ql of Punica granatum fruit seed oil (Pomegranate seed oil - L'Atelier d'Hippolyte) is mixed under continuous stirring with 1000 mg of ground freeze-dried Punica granatum fruit skin as obtained according to example 1.1.

[0302] A composition according to the invention comprising a mixture in the form of an oil adsorbed on an impregnated skin powder is obtained.

[0303] Example 2.3. Process for preparing the composition according to the present invention comprising a mixture in powder form.

[0304] A quantity of 100 ql of Punica granatum fruit seed oil (Pomegranate seed oil - L'Atelier d'Hippolyte) is mixed under continuous stirring with 1 g of ground freeze-dried Punica granatum fruit skin as obtained according to example 1.1. and 500 mg of SYLOID® (GRACE).

[0305] A composition according to the invention comprising a mixture in the form of an impregnated powder is obtained.

[0306] Example 2.4. Process for preparing the composition according to the present invention in semi-solid form.

[0307] An aqueous phase is prepared by mixing 0.05 g of cyclodextrin, 0.15 g of sorbitol syrup and water QSP 100 mL.

[0308] An oily phase is prepared in parallel by mixing 10 mg of the powder mixture as presented in Example 2.2 in MCT oil q.s. 100 mL.

[0309] The oily phase is gradually added to the aqueous phase over about 30 minutes and then mixed in a high-shear mixer.

[0310] A water-in-oil emulsion composition according to the invention is obtained.

[0311] The obtained composition is poured into a liquid specialty delivery device comprising a soft gelatin capsule reservoir and a cap that hermetically closes the reservoir, further comprising a tab disposed on the side opposite the cap relative to the reservoir, the tab being filled with soft gelatin as described in WO2021156557 (Al) application.

[0312] Example 3: Demonstration of the synergistic effect of the combination according to the invention.

[0313] The stabilization tests of bovine serum albumin (BSA, Cari Roth) to evaluate the anti-inflammatory and anti-arthritic potential of new drug candidates or natural extracts are conventionally used in the field.

[0314] Denatured proteins induce the synthesis of autoantigens and the resulting immune response. In addition, albumin is a transport protein that naturally circulates in the blood and has the ability to bind a wide variety of molecules, both water-soluble and lipid-soluble, which allows testing a broad spectrum of compounds.

[0315] In the present test, DMSO is used as a denaturing agent.

[0316] The experimental conditions of the present test requiring the use of solution, and not particles, the aqueous extract of crushed skin of the fruit of Punica granatum prepared according to the protocol described in example 1.2 is used. The interpretation of the results of the present example can be transposed to the skin of the fruit of Punica granatum in particulate form.

[0317] A first pre-incubation step at 37°C for at least 15 minutes of 10 ml of isotonic saline solution of BSA 5% (v / v) is carried out.

[0318] Four incubation solutions are prepared in DMSO and the indicated volumes are added to the 10 ml prepared above:

[0319] Sol. 1 (control): 100pL of pure DMSO.

[0320] Sol. 2: 1 00qL of solution comprising 20% ​​(v / v) of skin of the fruit of Punica granatum prepared according to the protocol described in example 1.2 for a final volume of solution 2 and DMSO QSP.

[0321] Sol. 3: 100qL of solution comprising 2% (v / v) of seed oil from the fruit of Punica granatum as described in example 2 for a final volume of solution 3 and DMSO QSP.

[0322] Sol. 4: 100qL of solution of the compound comprising 20% ​​(v / v) of Punica granatum fruit skin extract prepared according to the protocol described in Example 1.2, 2% (v / v) of Punica granatum fruit seed oil as described in Example 2, and DMSO QSP.

[0323] Sol. 4 therefore represents the condition corresponding to the composition according to the present invention.

[0324] The difference in DMSO concentration between condition 1 and conditions 2, 3 and 4 is negligible for the interpretation of the results.

[0325] The solutions are incubated at 37°C for 1 hour and vortexed every 10 minutes. The solutions are then set aside for 5 days.

[0326] Test tubes are prepared with 1960 μL of isotonic saline BSA solution so as to obtain a final BSA concentration of 0.1% (v / v), 750 qL of DMSO and 40 qL of one of the incubation solutions. The tubes thus prepared are immersed in a water bath at 70±2°C for 5 min, then left to cool at room temperature for 30 min.

[0327] Spectrophotometric analysis:

[0328] The image presented in [Fig. 1] [Fig. 1] shows the difference observed visually after 5 minutes of exposure of the sample to 70±2°C between the control (only BSA, tube on the left of the image) and the tested samples (BSA + active compound).

[0329] The absorbance is measured using a spectrophotometer (HeXios spectro-photometer) at 660nm. The absorbance results then obtained are shown in [Fig.2][Fig.2].

[0330] Treatment of BSA with Sol. 2 (Punica granatum fruit skin) reduces protein denaturation by 15% compared to the control.

[0331] Treatment of BSA with Sol.3 (Punica granatum fruit seed oil) reduced protein denaturation by 10% compared to the control.

[0332] Quite surprisingly, treatment of BSA with Sol. 4 (a mixture of skin and seed oil from the fruit of Punica granatum) leads to a reduction in protein denaturation of 32% compared to the control, a reduction greater than the sum of the reductions of the two compounds taken individually.

[0333] Thus, this study demonstrates the synergistic effect of the mixture of skin and seed oil of Punica granatum fruit in protecting the BSA protein against denaturation.

[0334] These results therefore demonstrate a synergistic effect of the mixture of Punica granatum fruit skin and Punica granatum fruit seed oil for the treatment of joint, bone, cartilage, inflammatory, cardiovascular and / or cognitive disorders.

Claims

Claims

1. A composition comprising pomegranate fruit skin, in particulate form or in extract form, and pomegranate fruit seed oil in a mixture.

2. The composition according to claim 1, wherein the pomegranate fruit skin in particulate form is a pomegranate fruit skin powder.

3. The composition according to any one of claims 1 or 2, wherein the pomegranate fruit skin in extract form is an aqueous extract of pomegranate fruit skin.

4. The composition according to any one of the preceding claims, wherein the pomegranate fruit skin is lyophilized.

5. The composition according to any one of the preceding claims, wherein the pomegranate fruit seed oil is obtained by cold pressing.

6. A composition according to any one of the preceding claims, wherein the mixture is characterized in that the ratio between the mass content of pomegranate fruit peel, in particulate form or in extract form, and the mass content of pomegranate fruit seed oil is in the range from 0.001 / 1 to 1000 / 1.

7. A composition according to any one of the preceding claims, wherein the mixture is characterized in that the ratio between the mass content of pomegranate fruit peel, in particulate form or in extract form, and the mass content of pomegranate fruit seed oil is in the range from 5 / 1 to 20 / 1.

8. Composition according to any one of the preceding claims, wherein the composition is a cosmetic, nutraceutical and / or pharmaceutical composition and / or a food ingredient and / or a food supplement and / or cosmetic, nutraceutical and / or pharmaceutical active ingredient.

9. Composition according to any one of the preceding claims, wherein the composition according to the present invention further comprises at least one pharmaceutically and / or cosmetically acceptable excipient.

10. Composition according to any one of claims 1 to 9 for its therapeutic and / or nutraceutical use.

11. Composition for use according to claim 10, for the treatment and / or prevention of joint, bone, cartilage, inflammatory, cardiovascular and / or cognitive disorders.

12. Composition for use according to any one of claims 10 or 11, for the treatment and / or prevention of atopic dermatitis, osteoarthritis, rheumatoid arthritis and / or osteoporosis and / or for maintaining and / or improving bone remodeling.

13. A delivery device comprising the composition of any one of claims 1 to 9.

14. The delivery device of claim 1 to 9, wherein the delivery device is a veterinary delivery device.

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