Fish protein hydrolysate for muscle development in foals and horses

Abstract

FISH PROTEIN HYDROLYSATE FOR MUSCLE DEVELOPMENT IN FOALS AND HORSES The present invention relates to the use of a protein hydrolysate derived from collagen-rich fish tissues or cartilage to promote muscle development or maintain muscle mass in horses or foals. According to the invention, the hydrolysate has a protein fraction with the following molecular distribution profile: between 15 and 60% peptides with a molecular weight between 1000 and 5000 Da, between 20 and 40% peptides with a molecular weight between 500 and 1000 Da, between 10 and 40% peptides with a molecular weight between 150 and 500 Da, and between 1 and 10% peptides with a molecular weight less than 150 Da. Fig. 1

Description

Title of the invention: PROTEIN HYDROLYSAT FISH FOR MUSCLE DEVELOPMENT IN FOALS AND HORSES technical field

[0001] The present invention is in the field of animal welfare and relates to the use of a protein hydrolysate from protein-rich fish tissues or cartilage, in particular collagen-rich, to promote the growth and development of the foal, maintain or strengthen the muscle mass of horses or foals and improve their physical appearance. PREVIOUS STATE OF THE ART

[0002] After birth, the foal exhibits rapid growth and development. During the first few months, it is fed its mother's milk. Around three to four months of age, during the weaning period, the foals' diet is enriched with solid food. Between one and two years of age, growth slows down. They finally reach their adult size between four and five years of age.

[0003] During this period of growth and development, weight gain must be regular to ensure optimal development, in particular to guarantee optimal Average Daily Gain (ADG). ADG, expressed in kilograms per day, is a growth indicator used in animal husbandry.

[0004] For muscle mass development, nutrition is a key factor. Muscle fibers are composed of protein. A horse therefore needs protein to develop its muscles. This is why it is beneficial to supplement the animal's diet with a protein source, in particular one with an amino acid profile suitable for muscle development.

[0005] In adult horses, maintaining and strengthening muscle mass is also essential. In racehorses in particular, muscles are subjected to significant stress. Regular exercise and an appropriate diet help maintain or increase muscle mass. Description of the invention

[0006] Whether dealing with growing foals or adult horses for sport or leisure, muscle development, strengthening, maintenance, and physical appearance are essential factors. Indeed, the animal's physical condition, including its weight, height, and coat quality, are indicators of its health or selection criteria. In some cases, it may be necessary to stimulate or strengthen muscle mass development and increase average daily gain (ADG). in order to support or promote the growth and development of the foal or to help the adult horse recover after exertion and to increase its sporting abilities such as its running speed or power.

[0007] The aim of the present invention is therefore to offer a natural and effective solution to stimulate or aid the growth and development of the foal, develop, maintain or strengthen the muscle mass of foals or horses, promote or improve their physical appearance, improve the recovery of the horse after exertion and strengthen or maintain its sporting abilities.

[0008] To this end, the invention relates to the use of a protein hydrolysate of fish collagen-rich tissues or cartilage, or a composition comprising theresuch, said hydrolysate having a protein fraction having the following molecular distribution profile: - between 15 and 60% of peptides with a molecular weight between 1000 and 5000 Da, - between 20 and 40% of peptides with a molecular weight between 500 and 1000 Da, - between 10 and 40% of peptides with a molecular weight between 150 and 500 Da, - between 1 and 10% of peptides with a molecular weight less than 150 Da,

[0009] and an amino acid profile in which, as a percentage of the total weight of amino acids: - The glutamic acid content is between 10 and 14% - The valine content is between 1.5 and 3.5% - the isoleucine content is between 1 and 3% - The leucine content is between 2 and 6% - The glycine content is between 9 and 13% - The lysine content is between 0.5 and 3% - the arginine content is between 6 and 10%,

[0010] to promote the growth and development of a foal, to maintain, develop or strengthen the muscle mass of a horse or foal, to improve the physical appearance of a horse or foal.

[0011] In the context of the invention, "growth" means the increase in live weight with age. It is expressed as Average Daily Gain, in kilograms per day.

[0012] The term “development” means the progressive attainment of the adult state resulting from changes in the conformation, chemical composition and functions of the various tissues and body regions.

[0013] The term "horse" means an animal that has reached adulthood and the term "foal" means an animal that is still growing.

[0014] The hydrolysate according to the invention is a mixture of peptides and free amino acids obtained after enzymatic hydrolysis, using a specific enzyme, of raw materials from fish, such as fish skin and cartilage. Since these materials are rich in collagen, the peptides and free amino acids that make up the hydrolysate are primarily derived from collagen.

[0015] The hydrolysate according to the invention is thus particularly effective in stimulating muscle development in growing foals, thereby promoting their growth and development and in strengthening the muscle mass of horses in adulthood.

[0016] It should also be noted that the hydrolysate according to the invention is advantageously rich in growth hormone precursor amino acids such as arginine, valine, glutamic acid, lysine, and long-chain amino acids (BCAAs), namely leucine, isoleucine, and valine. Growth hormone increases muscle volume, increases bone density, and decreases fat synthesis.

[0017] Thus, advantageously, the use according to the invention includes increasing the average daily gain of the foal or horse.

[0018] Advantageously, the use according to the invention includes the improvement of the condition of the hair, in particular the improvement of shine.

[0019] Advantageously, the use according to the invention includes improving the recovery of the horse or foal after exertion and increasing its sporting abilities.

[0020] Advantageously, the hydrolysate also presents:

[0021] - a fat content of less than 0.5% by weight percentage of extract dry,

[0022] - a total nitrogen content (N * 6.25) greater than 90%, as a percentage weight of dry extract,

[0023] - a mineral content of less than 10%, as a percentage by weight of extract dry.

[0024] Preferably, the amino acid profile also presents, as a percentage of the total weight of amino acids:

[0025] - an aspartic acid content of between 7 and 10%

[0026] - a proline content of between 7 and 11%

[0027] - a threonine content of between 2 and 5%

[0028] - a serine content of between 3 and 7%

[0029] - an alanine content of between 8 and 12%

[0030] - a cystine content of between 0.1 and 1%

[0031] - a methionine content of between 1.5 and 3%

[0032] - a tyrosine content of between 0.5 and 2.5%

[0033] - a phenylalanine content of between 1 and 3.5%

[0034] - a histidine content of between 3.5 and 7%

[0035] - a hydroxyproline content of between 4 and 8%

[0036] - a hydroxylysine content is between 0.5 and 2.5%.

[0037] The hydrolysate used in the context of the invention is obtained by enzymatic digestion using a bacterial endopeptidase enzyme, advantageously a bacterial endoprotease marketed under the brand name Corolase 7089 marketed by the company AB enzymes.

[0038] In particular, the hydrolysate can be obtained by a process comprising the steps of:

[0039] - preparation of a protein source from fish tissues and / or cartilage rich in collagen,

[0040] - preparation of an aqueous enzymatic solution comprising between 0.1 and 0.5%, in weight of an endoprotease enzyme,

[0041] - enzymatic hydrolysis of said protein source at a temperature including between 40 and 60°C, for 1 to 5 hours, after the addition of the enzyme solution,

[0042] - stopping enzymatic hydrolysis by heating to a temperature of at least 90°C for 8 to 20 minutes,

[0043] - separation of the protein hydrolysate from the other components,

[0044] - recovery and concentration of protein hydrolysate.

[0045] Advantageously, the ratio, by volume, of protein source / water is less than or equal to 1.

[0046] Preferably, the tissue is skin.

[0047] Preferably, the fish is a Salmonidae, preferably salmon or trout, or a white fish.

[0048] In the context of the invention, the protein hydrolysate may be included in a composition. This composition may be in any form known and suitable for feeding foals or horses, such as a solution, a solid composition such as a tablet, powder or capsule, a granule or a gel. The composition may include any suitable excipient, and any other compound such as vitamins, minerals, active ingredients, preservatives, or colorants.

[0049] Advantageously, the hydrolysate, or the composition containing it, is administered to foals or horses at a rate of 20 to 30 g of hydrolysate per day per 100 kg of body weight, for a hydrolysate having a dry extract content between 20 and 30% by weight. When dealing with a growing foal, administration is preferably carried out between the ages of 1 and 2 years.

[0050] Starting from the necessary daily dosage, a person skilled in the art will be able to formulate a composition adapted to such a dosage.

[0051] The features of the invention mentioned above, as well as others, will become clearer upon reading the following examples and in connection with the accompanying drawing below:

[0052] [Fig-1] is a diagram representing the distribution of peptides by classes of a protein hydrolysate according to the invention, as a function of their molecular weights.

[0053] EXAMPLES

[0054] Example 1: obtaining protein hydrolysates from salmon skin

[0055] Four salmon skin protein hydrolysates were prepared according to the following process: - Mixture of salmon skins with 1 volume of water containing 0.2%, by weight, of Corolase 7089® enzyme - Enzymatic hydrolysis lasting 1 to 5 hours at a temperature of 40 to 60°C, - Inactivation of the enzyme by heating to a temperature of at least 90°C for 8 to 20 minutes, - Sieving through a 300 µm sieve to remove hydrolysis residues, - Centrifugation of the hydrolysis juice for 10 to 15 minutes at 4700 rpm to separate the oil, insolubles, and protein hydrolysate. - Concentration of the hydrolysate.

[0056] Table 1 indicates the characteristics of the hydrolysates.

[0057] [TAB. 1] Parameters Composition dry extract Dry extract (%) 100% Total protein (N*6.25) >90% Fat <0.5% Minerals <10%

[0058] Fig. 1 shows the distribution of molecular weights of the four hydrolysates. Example 2#: Evaluation# on foals

[0059] A hydrolysate according to example 1 was administered to five foals of age between 1 and 2 year olds, at a dose of 25g / day / 100kg of body weight for 50 to 60 days. Average Daily Gain (ADG, in kg / day) and coat appearance were assessed and compared to those of control foals that did not receive the hydrolysate.

[0060] It has been observed that administration of the hydrolysate according to the invention resulted in average daily gains 10% greater than those of the control group. The Body Condition Score (BCS), a reference assessment grid established by the IFCE (French Institute of the Horse and Riding), consists of evaluating the mass of adipose tissue based on visual observations and palpation of anatomical areas. including the ribs, the back of the shoulder, the withers, the upper edge of the neck, the tail attachment and the croup, is improved by 1 to 2 points on average to go from 2 to 3, or even to 4 (out of 5 points in total).

[0061] Furthermore, a much better condition of the hair was noted in the foals that received the hydrolysate, in particular a visibly more pronounced shine. Example 3#: Evaluation# on adult horses

[0062] A hydrolysate according to example 1 was administered to emaciated adult horses at a rate of 25 g / day / 100 kg body weight for 13 days. An average daily gain (ADG, in kg / day) of 1 kg was observed.

Claims

Demands

1. Use of a protein hydrolysate of collagen-rich fish tissue or cartilage, or a composition comprising such hydrolysate, having a protein fraction with the following molecular distribution profile: - between 15 and 60% peptides with a molecular weight between 1000 and 5000 Da, - between 20 and 40% peptides with a molecular weight between 500 and 1000 Da, - between 10 and 40% peptides with a molecular weight between 150 and 500 Da, - between 1 and 10% peptides with a molecular weight less than 150 Da, and an amino acid profile wherein, as a percentage by total weight of amino acids: - the glutamic acid content is between 10 and 14%, - the valine content is between 1.5 and 3.5%, - the isoleucine content is between 1 and 3%, - the leucine content is between 2 and 6%, - the glycine content is between 9 and 13%,- The lysine content is between 0.5 and 3%, - the arginine content is between 6 and 10%, to promote the growth and development of the foal, maintain, develop or strengthen the muscle mass of the horse or foal, improve the physical appearance of the horse or foal.

2. Use according to claim 1, for increasing the average daily gain of the foal or horse.

3. Use according to claim 1, for improving the condition of the hair, in particular for improving shine.

4. Use according to any one of the preceding claims, characterized in that the hydrolysate further has: - a fat content of less than 0.5% by weight percentage of dry extract, - a total nitrogen content (N * 6.25) of more than 90% by weight percentage of dry extract, - a mineral content of less than 10%, as a percentage by weight of dry extract.

5. Use according to any one of the preceding claims, characterized in that the amino acid profile further presents, as a percentage by total weight of amino acids: - an aspartic acid content of between 7 and 10% - a proline content of between 7 and 11% - a threonine content of between 2 and 5% - a serine content of between 3 and 7% - an alanine content of between 8 and 12% - a cystine content of between 0.1 and 1% - a methionine content of between 1.5 and 3% - a tyrosine content of between 0.5 and 2.5% - a phenylalanine content of between 1 and 3.5% - a histidine content of between 3.5 and 7% - a hydroxyproline content of between 4 and 8% - a hydroxylysine content of between 0.5 and 2.5%.

6. Use according to any one of the preceding claims, characterized in that said fabric is skin.

7. Use according to any one of the preceding claims, characterized in that said fish is a Salmonidae, preferably salmon or trout, or a whitefish.

8. Use according to any one of the preceding claims, characterized in that the hydrolysate is obtained by enzymatic digestion using an endopeptidase enzyme of bacterial origin, preferably the enzyme marketed under the brand name Corolase 7089.

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