Laser adapter, multiphoton microscope main unit an optical system

Abstract

The present application provides a laser adapter, a multiphoton microscope main unit and an optical system. The laser adapter comprises a shell, and a beam transformation device and a beam stabilization device, which are arranged in the shell, wherein the shell is provided with a laser input port and a laser output port; the beam transformation device is configured to transform a laser beam that enters the shell; and the beam stabilization device is arranged downstream of the beam transformation device in a laser transmission direction, and is used for adjusting the laser transmission direction, so as to correct the deviation between an actual position and ideal position of the laser beam at the laser output port. By means of the technical solution provided by the present application, when lasers with different parameters are input or a laser deflects during transmission, there is no need to readjust an optical path or replace an optical device on the optical path, and simply by means of the transformation of the input laser and the adjustment of the laser transmission direction by the laser adapter, the laser can be adapted and coupled to an apparatus connected therebehind.

Description

Laser adapters, multiphoton microscope mainframes and optical systems Technical Field

[0001] The present application relates to the field of optical technology, and in particular to a laser adapter, a multi-photon microscope host and an optical system.

[0002] Background of the Invention

[0003] In the laser coupling scheme of the existing technology, an adjustment mechanism with multiple degrees of freedom is usually used. A lens is set on the adjustment mechanism. By adjusting the lens, the spatial laser is coupled into the optical fiber. In order to ensure the coupling efficiency of the laser, it is usually necessary to select the lens based on the laser output spot size, light waist position, etc.

[0004] However, if the laser is replaced or the spot size is changed, the optical path needs to be readjusted or the lens needs to be reselected. The optical path adjustment and lens replacement are complicated and require professional operation.

[0005] In addition, if the environment changes, such as vibration or temperature changes, the output pointing angle of the laser will also change. The typical laser is 25μrad / ℃. If the temperature changes by 10℃, the pointing angle changes by 250μrad. The laser pointing direction changes, and the coupling efficiency of the optical fiber will drop sharply, greatly affecting the performance of the equipment. Moreover, if the light beam is deflected due to human error, the system will also need to be re-debugged.

[0006] Summary of the Invention

[0007] In view of this, the present application provides a laser adapter, a multiphoton microscope host and an optical system to solve the problems existing in the laser coupling scheme in the prior art, such as the complexity of optical path adjustment and lens replacement, and the decrease in laser coupling efficiency caused by the easy change of laser.

[0008] According to one aspect of the present application, a laser adapter is provided, comprising: a housing and a beam conversion device and a beam stabilization device disposed within the housing; the housing having a laser input port and a laser output port; the beam conversion device being configured to convert a laser beam entering the housing; and the beam stabilization device being disposed downstream of the beam conversion device along a laser transmission direction, and configured to adjust the laser transmission direction to correct a deviation between an actual position of the laser beam at the laser output port and an ideal position.

[0009] According to another aspect of the present application, the present application provides a multiphoton microscope host, which is used to solve the problems of multiphoton microscopes in the prior art, such as large space occupation, difficulty in transportation and transfer, and complex installation and maintenance. The multiphoton microscope host provided by the present application is used to connect to a microscope probe, and the multiphoton microscope host includes a mounting body and a wide-field search module, a laser coupling module, a fluorescence collection module and a scanning control module integrated on the mounting body; wherein the wide-field search module is configured to perform wide-field imaging of a living body to search for a target area for installing a microscope probe on the living body; the laser coupling module is configured to receive laser light and adjust the laser light to couple the laser light into a laser transmission fiber, wherein the laser transmission fiber is used to connect the laser coupling module and the microscope probe; the scanning control module is configured to be connected to the microscope probe via a control cable, and is used to control the microscope probe to perform laser scanning to generate a fluorescence signal; the fluorescence collection module is configured to be connected to the microscope probe via a fluorescence collection fiber, and is used to collect the fluorescence signal output by the microscope probe.

[0010] According to another aspect of the present application, an optical imaging system is provided to address issues in conventional optical imaging systems, such as changes in the laser light emitted by the laser, which require readjustment of the optical path and result in poor flexibility. The present application provides an optical system comprising a laser, a transmission fiber, an application device, and the laser adapter described above; wherein the laser is disposed at the laser input port of the laser adapter for emitting laser light toward the laser input port; one end of the transmission fiber is connected to a laser coupler, the laser coupler being connected to the laser output port; and the other end of the transmission fiber is connected to the application device.

[0011] According to another aspect of the present application, an optical system is also provided, which includes: a laser, a laser adapter and a microscope host; wherein the laser is used to emit laser light to the laser adapter; the laser adapter is used to receive the laser light emitted by the laser, adjust and adapt the laser light, and then transmit the adjusted and adapted laser light to the microscope host; the microscope host is configured to transmit the laser light to a microscope probe, and control the microscope probe to perform laser scanning on a living organism to generate a fluorescence signal for imaging.

[0012] In the technical solution provided by the present application, the laser adapter is provided with a beam conversion device and a beam stabilization device. The beam conversion device can convert the input laser beam so that the laser can match the subsequent equipment and achieve the optimal performance of the equipment. The beam stabilization device can adjust the deflection direction of the laser when it detects that the laser beam is offset, ensuring the stability of the laser output and thus ensuring the coupling efficiency of the laser output. Therefore, when using the laser adapter provided by the present application, when a laser with different parameters is input or when deflection occurs during the laser transmission process, there is no need to readjust the optical path or replace the optical devices on the optical path. The laser adapter can transform the input laser and adjust the laser transmission direction to make the laser adaptable and coupled to the subsequent connected equipment.

[0013] The multiphoton microscope mainframe integrates various functional modules into a single structure, significantly reducing space usage and making it suitable for various laboratories. Furthermore, when configured as a complete unit, the draw lines are neat and tidy. Furthermore, due to its small size and portability, the multiphoton microscope mainframe is easy to transport to other locations. The position and orientation of the multiphoton microscope can be quickly adjusted to suit various experimental needs, facilitating its adaptation to a wide range of applications. Furthermore, the multiphoton microscope mainframe facilitates rapid on-site installation and maintenance.

[0014] In the optical system provided herein, the laser light generated by the laser passes through a laser adapter, which can perform transformations such as amplification, reduction, and zooming on the laser beam, converting the various received laser signals into a unified laser signal output, thereby adapting the laser to subsequently connected devices and facilitating optimal system performance. This allows the use of lasers with various parameters, and even if the distance of the laser changes, the laser adapter can still output an adapted laser beam to the microscope host after transforming the received laser light.

[0015] Other features and advantages of the present application will be described in detail in the subsequent detailed description.

[0016] BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The accompanying drawings, which constitute part of this application, are intended to provide a further understanding of this application. The exemplary embodiments and descriptions of this application are intended to explain this application and do not constitute an improper limitation on this application. In the accompanying drawings:

[0018] FIG1 is a schematic diagram of the appearance of a laser adapter according to one embodiment of the present application;

[0019] FIG2 is a schematic structural diagram of a laser adapter with the upper cover removed according to an embodiment of the present application;

[0020] FIG3 is a schematic structural diagram of an optical system according to an embodiment of the present application;

[0021] FIG4 is a schematic diagram of an optical path of an optical system according to an embodiment of the present application;

[0022] FIG5 is a schematic diagram of the appearance structure of a multiphoton microscope host according to one embodiment of the present application;

[0023] FIG6 is a schematic structural diagram of the multiphoton microscope main unit shown in FIG5 with the light shielding door in an open state;

[0024] FIG7 is a schematic structural diagram of a multiphoton microscope host in a disassembled state according to one embodiment of the present application;

[0025] FIG8 is a schematic structural diagram of the multiphoton microscope main unit in a disassembled state as viewed from another angle;

[0026] FIG9 is a schematic diagram of the coordinated installation of a mobile module, a living body installation device, and a field of view search adapter according to one embodiment of the present application;

[0027] FIG10 is a schematic structural diagram of a living body installation device according to one embodiment of the present application;

[0028] FIG11 is a schematic structural diagram of a laser coupling module according to one embodiment of the present application;

[0029] FIG12 is a schematic diagram of the internal structure of the laser coupling module shown in FIG11;

[0030] FIG13 is a schematic diagram showing a structure in which a wide-field search module is installed on a laser coupling module according to an embodiment of the present application;

[0031] FIG14 is a schematic structural diagram of a control box according to one embodiment of the present application;

[0032] FIG15 is a front view of the control box shown in FIG14;

[0033] FIG16 is a schematic structural diagram of a storage device according to an embodiment of the present application;

[0034] FIG17 is a schematic structural diagram of a storage device in a disassembled state according to one embodiment of the present application;

[0035] FIG18 is a schematic structural diagram of an optical system according to one embodiment of the present application;

[0036] FIG19 is a schematic structural diagram of an optical system according to another embodiment of the present application.

[0037] Explanation of reference numerals: 100 - multiphoton microscope main unit; 1 - mounting body; 11 - base; 12 - mounting bracket; 13 - support plate; 14 - handle; 15 - display screen; 2 - light shielding door; 3 - laser coupling module; 31 - input end; 32 - output end; 311 - power detector; 33 - coupler housing; 331 - optical path through hole; 34 - dispersion compensation element; 35 - reflector; 36 - acousto-optic modulator; 361 - driver; 362 - heat sink; 363 - fan; 37 - first deflecting reflector; 38 - second deflecting reflector; 39 - position detector; 4 - field of view search module; 41 - fluorescent light source; 42 - camera; 43 - objective lens; 5 - control box; 51 -First interface; 52 -Second interface; 53 -Fluorescence collection module; 54 -Main control circuit board; 6 -Storage device; 61 -Storage box; 611 -Protrusion; 612 -Through hole; 613 -Annular groove; 614 -Through groove; 62 -Annular indicator light; 63 -Wire retaining reel; 631 -Light-transmitting cover; 632 -Wire retaining ring; 633 -Winding drum; 64 -Wire clamping ring; 643 -Clamping slot; 65 -Probe bracket; 66 -Protective cover; 661 -Annular cover; 662 -Observation window; 67 -Hinge; 68 -Hinge; 7 -Mobility module; 8-In vivo mounting device; 81-Mounting seat; 811-Baffle; 812-Fixed frame; 82-Clamping assembly; 821-First adjusting bolt; 83-Light shield; 84-Rotating component; 85-Moving frame; 86-Second adjusting bolt; 87-Third adjusting bolt; 88-Treadmill; 89-Receiving tray; 80-Probe mounting part; 9-Field search adapter; 91-Probe mounting assembly; 10-Cover; 200-Laser; 300-Laser adapter; 301-Transmission fiber; 400-Microscope probe; 401- Laser transmission optical fiber; 402-fluorescence collection optical fiber; 403-control cable; 3001-housing; 302-first fixed reflector; 303-second fixed reflector; 304-beam conversion gas; 305-first deflection reflector; 306-second deflection reflector; 307-position detector; 308-laser power meter; 309-beam splitter; 3010-switching mechanism; 3011-laser input port; 3012-laser output port; 3013-support leg; 3014-laser coupler; 3016-driving circuit.

[0038] Modes for Carrying Out the Invention

[0039] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of this application. In the absence of conflict, the embodiments in the present application and the features in the embodiments can be combined with each other.

[0040] In the description of this application, it should be understood that the terms "center," "longitudinal," "lateral," "upper," "lower," "left," "right," "vertical," "horizontal," "top," "bottom," "axial," "radial," "circumferential," and the like, indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings and are intended solely to facilitate the description of this application and simplify the description. They do not indicate or imply that the devices or components referred to must have a specific orientation, be constructed, or operate in a specific orientation. Therefore, they should not be construed as limitations on this application. Furthermore, "inside" and "outside" refer to the inside and outside relative to the outline of each component itself.

[0041] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be understood to indicate or imply relative importance or implicitly specify the quantity of the technical features indicated. Thus, features defined as "first" or "second" may explicitly or implicitly include at least one of these features.

[0042] The present application provides a laser adapter, as shown in Figures 1 and 2. The laser adapter 300 includes: a housing 3001 and a beam conversion device 304 and a beam stabilization device disposed in the housing 3001; wherein the housing 3001 has a laser input port 3011 and a laser output port 3012; the beam conversion device 304 is configured to convert the laser beam entering the housing 3001; the beam stabilization device is disposed downstream of the beam conversion device 304 along the laser transmission direction, and is configured to adjust the deflection direction of the laser beam to correct the deviation between the actual position of the laser beam at the laser output port 3012 and the ideal position.

[0043] The laser adapter provided herein can transform the input laser beam through a beam transformation device 304. Beam transformation refers to the process of amplifying, reducing, and zooming the beam through the transformation properties of optical elements, enabling the laser to be matched with subsequent equipment and achieving optimal performance. Furthermore, the laser adapter provided herein can adjust the deflection direction of the laser beam when a deviation in the laser beam is detected, thereby stabilizing the laser output and ensuring the coupling efficiency of the laser output.

[0044] Specifically, when using lasers with different parameters, when the lasers emit lasers with different parameters and enter the laser adapter, the beams can be transformed by the beam conversion device to uniformly output beams with a fixed spot size, so that the lasers output by different lasers can all be adapted to the devices connected later; or, if the distance of the lasers changes, a uniform laser beam can also be output after zooming through the beam conversion device 304.

[0045] When the environment changes (such as changes in temperature, humidity, etc., or vibration occurs), the various devices on the optical path (such as the laser itself, reflectors, spectrometers, etc.) will be vibrated or offset due to the influence of temperature, causing the output direction of the laser to change. The beam stabilization device can adjust the deflection direction of the laser in real time according to the deviation between the actual position of the light beam at the laser output port 3012 and the ideal position, so that the laser output is stable, that is, the laser is controlled to be output within a range with a small deviation from the ideal output position, thereby ensuring the coupling efficiency of the laser output.

[0046] Therefore, by using the laser adapter provided in the present application, when a laser with different parameters is input or a deflection occurs during the laser transmission process, there is no need to readjust the optical path or replace the optical devices on the optical path. The laser adapter can be used to transform the input laser and adjust the laser transmission direction so that the laser can be adapted and coupled to the device connected later.

[0047] In one embodiment, the beam transformation device 304 may be an existing device capable of expanding or reducing the cross section of a laser beam and zooming the laser beam. The specific structure thereof can be implemented by those skilled in the art and will not be described in detail here.

[0048] The beam stabilization device is located downstream of the beam transformation device 304 in the laser transmission direction. In this way, the beam stabilization device can correct the beam deflection caused by the laser beam being magnified, reduced or zoomed by the beam transformation device 304.

[0049] As shown in FIG2 , the beam stabilization device may include a position detector 307, at least one deflecting reflector, and a reflector adjustment mechanism connected to each deflecting reflector. The position detector 307 is positioned near the laser output port 3012 and is used to detect the position information of the laser at the laser output port 3012. Specifically, a beam splitter 309 may be provided along the laser transmission path, and the beam splitter 309 directly or indirectly reflects a portion of the laser light to the position detector 307, thereby enabling the position detector 307 to detect the laser position. In the embodiment shown in FIG17 , the beam splitter 309 reflects a portion of the laser light to the laser power meter 308, which also includes a beam splitter. The laser power meter reflects a portion of the laser light to the position detector 307. The position detector 307 may employ a 4D position detector, which can strictly detect and distinguish between position drift and angle drift of the light beam and accurately detect the real-time position of the light beam.

[0050] The reflector adjustment mechanism is configured to drive the deflection reflector to deflect according to the position information detected by the position detector 307 to adjust the laser transmission direction so that the laser is output stably.

[0051] Specifically, the ideal position of the laser beam at the laser output port 3012 is first determined. This ideal position is the position at which the laser beam can achieve ideal coupling efficiency when it is output and coupled into a device or component (e.g., a transmission optical fiber) connected to the laser output port 3012. When the beam deflects, for example, due to vibration or temperature change causing an optical device to shift, or due to human touch, the position detector 307 detects the position information of the laser beam at the laser output port 3012 in real time and sends it to the control unit. The control unit continuously determines the deviation between the position of the laser beam and the ideal position based on the position information, and controls the reflector adjustment mechanism to adjust the deflection reflector, thereby continuously adjusting the reflection direction of the laser beam, so that the laser beam is stably transmitted within a certain range around the ideal position.

[0052] The laser adapter also includes a control unit that receives position information detected by the position detector 307 and controls the reflector adjustment mechanism based on the position information. In some embodiments, the control unit can be disposed within the housing 3001. Alternatively, the control unit can be disposed separately, i.e., as a separate module disposed outside the housing 3001.

[0053] As shown in FIG2 , a driving circuit 3016 is further provided in the housing 3001 of the laser adapter. The control unit sends a control signal to the driving circuit 3016 , and the driving circuit 3016 controls the operation of the reflector adjustment mechanism.

[0054] In one embodiment, the laser adapter 300 further includes at least one fixed reflector for changing the direction of laser transmission. The fixed reflector is positioned upstream of the beam transforming device 304 along the laser transmission direction. By providing a fixed reflector to change the direction of laser transmission, the optical path can be bent, facilitating the placement of components along the optical path and reducing the overall size of the laser adapter.

[0055] In the embodiment shown in FIG2 , the at least one fixed reflector includes a first fixed reflector 302 and a second fixed reflector 303 , and the at least one deflecting reflector includes a first deflecting reflector 305 and a second deflecting reflector 306 ;

[0056] The laser is reflected by the first fixed reflector 302 to the second fixed reflector 303, and the second fixed reflector 303 reflects the laser to the beam conversion device 304; after the beam conversion device 304 converts the laser, the output laser is reflected by the first deflecting reflector 305 to the second deflecting reflector 306, and the second deflecting reflector 306 is configured to reflect the laser to the laser output port 3012.

[0057] More specifically, the incident angle and the exit angle of the laser at the first fixed reflector 302 and the incident angle and the exit angle of the laser at the second fixed reflector 303 are approximately 45° respectively, and the incident angle and the exit angle of the laser at the first deflecting reflector 305 and the incident angle and the exit angle of the laser at the second deflecting reflector 303 are approximately 45° respectively.

[0058] Referring to Figure 3 , a laser transmission path is shown. Laser light emitted by laser 200 enters through laser input port 3011, is transmitted to first fixed reflector 302, deflected approximately 90 degrees by first fixed reflector 302, and then reflected to second fixed reflector 303. Further deflected approximately 90 degrees by second fixed reflector 303, the laser light is transmitted to beam converter 304, and after beam conversion by beam converter 304, the laser light is transmitted to first deflecting reflector 305, deflected approximately 90 degrees by first deflecting reflector 305, and then reflected to second deflecting reflector 306. The laser light is then reflected by second deflecting reflector 306 to laser output port 3012 and coupled into a component connected to laser output port 3012, such as transmission fiber 301 connected to laser output port 3012 via laser coupler 3014.

[0059] In this embodiment, the optical path is bent by means of a fixed reflector and a deflecting reflector, which can reduce the length of the laser adapter while facilitating the arrangement of various optical components.

[0060] It can be understood that the arrangement of the fixed transmitting mirror and the deflecting reflecting mirror is not limited to the above-mentioned arrangement, and other arrangements are also possible.

[0061] In one embodiment, at least one laser power meter 308 is disposed within the housing 3001 to detect the power of the laser entering the laser adapter. The laser power meter 308 can detect changes in laser power in real time, thereby determining whether there are any problems with laser transmission, particularly problems with the laser input, such as damage to the laser or laser obstruction.

[0062] Optionally, the laser power meter 308 is disposed near the laser output port 3012 and can detect the power of the laser when it is output from the laser adapter.

[0063] Specifically, as shown in FIG3 , a spectroscope 309 is provided on the laser transmission path. The spectroscope 309 can split a portion of the laser beam and reflect it to the laser power meter 308 . The laser power meter 308 can obtain the power of the laser by detecting the split beam.

[0064] Optionally, the at least one laser power meter includes a first laser power meter and a second laser power meter. The first laser power meter can be located near the laser input port 3011, and the second laser power meter can be located near the laser output port 3012. That is, the first laser power meter detects the power of the laser during input, and the second laser power meter detects the power of the laser during output. By detecting changes in laser power during input, it is possible to determine whether there is a problem with the laser input, such as whether the laser is damaged or blocked. The first laser power meter and the second laser power meter respectively detect the power of the laser during input and output, and the changes in laser power during output compared to laser input can be compared, thereby determining laser power loss within the laser adapter.

[0065] In one embodiment, the laser adapter 300 further includes a switch device 3010 disposed at the laser input port 3011 , the switch device 3010 including a switch door for opening and closing the laser input port 3011 and a door driving mechanism for driving the switch door to switch between an open state and a closed state;

[0066] When the switch door is open, the laser can enter the laser adapter 300 for transmission. When the switch door is closed, the laser is blocked from entering the laser adapter.

[0067] The door driving mechanism can be controlled by a control unit. The control unit sends a control signal, and the driving circuit 3016 can control the door driving mechanism to drive the door to open or close.

[0068] In addition, a support leg 3013 can be set under the shell 3001 of the laser adapter 300. The support leg 3013 is set to be height-adjustable. By adjusting the height of the support leg 3013, the laser input port 3011 can be adapted to the height of the laser, so that the laser can accurately emit laser light to the laser input port 3011.

[0069] Among them, the height adjustment of the support leg 3013 can adopt existing common technologies, such as adjusting the height of the support leg 3013 by setting an adjustment bolt, or setting the support leg to include two parts, and adjusting the height by connecting the two parts at different height positions.

[0070] Another embodiment of the present application also provides an optical system. Directly recording neuronal activity in freely moving living animals is one of the most direct and effective methods for studying the relationship between animal behavior and neural function. Multiphoton optical imaging systems, with their excellent optical sectioning capabilities and deep penetration depth, have become the most important and widely used tools for neuronal observation. Among them, multiphoton optical imaging systems can be nonlinear laser scanning microscopes such as two-photon, three-photon, and Raman lasers.

[0071] In traditional multi-photon optical imaging systems, the laser and optical adjustment frame are fixed on an optical platform to adjust the optical path. After the optical path is shaped, it enters the microscope host through a reflector. Because the optical path from the laser to the microscope host is a spatial optical path, the microscope host must also be stably fixed on the optical platform to ensure that the optical path inside the main body will not be deflected by external forces, thereby affecting the performance of the microscope.

[0072] However, since many modules are usually placed around it, such as beam shaping, circuit control modules, various drivers, fluorescence collection modules, wide-field fluorescence modules, laser modules, etc., the equipment is complicated and the wiring is complex. In addition, the modules are easily affected by signal interference and human misoperation, which can easily cause the optical path to deflect.

[0073] Furthermore, the optical path and microscope are fixed, making it impossible to adapt or implement experiments that require the microscope's position and orientation. For example, if the laser optical path and the microscope are not on the same platform or even in the same room, traditional methods are impossible to implement. If the laser is replaced or the distance between the lasers changes, the laser light emitted by the laser will change, requiring readjustment of all optical paths. Some experiments may even be impossible to adapt due to the large differences in laser parameters.

[0074] The present application provides an optical system, as shown in FIG3 , comprising a laser 200, a transmission optical fiber 301, an application device 100, and the laser adapter 300 mentioned in the above-mentioned embodiments. The application device 100 can be a laser-based device such as a two-photon microscope or a microscope host. The laser 200 is configured to emit laser light toward the laser adapter 300. The laser adapter 300 is configured to receive the laser light emitted by the laser 200, adjust and adapt the laser light, and then transmit the adjusted and adapted laser light to the application device 100. The application device 100 is configured to transmit the laser light to a microscope probe and control the microscope probe to perform laser scanning on a living organism to generate a fluorescence signal for imaging.

[0075] In the technical solution provided by this application, the laser light generated by the laser 200 passes through the laser adapter 300. The laser adapter 300 can perform transformations such as amplification, reduction, and zooming on the laser beam, converting the various received laser signals into a unified laser signal output, making the laser compatible with subsequently connected devices and facilitating optimal system performance. This allows the use of lasers with various parameters, and even if the distance between the lasers changes, the laser adapter 300 can still output an adapted laser beam to the application device body 100 after transforming the received laser light.

[0076] As shown in FIG. 4 , the optical system may further include a microscope probe 400 , and the application device body 100 may be a multi-photon microscope host.

[0077] Among them, the laser 200 is arranged at the laser input port 3011 of the laser adapter 300, and is used to emit laser to the laser input port 3011; one end of the transmission optical fiber 301 is connected to the laser coupler 3014, the laser coupler 3014 is connected to the laser output port 3011, and the other end of the transmission optical fiber 301 is connected to the application device body 100.

[0078] The laser is stably coupled to the transmission optical fiber 301 through the laser adapter 300, and then transmitted to the application device body 100 through the transmission optical fiber 301. In addition, the lasers of various parameters emitted by the laser 200 can be adjusted to adapt to the application device body, ensuring that the application device body is not affected by parameter changes of the laser 200 or changes in the distance between the laser 200 and the laser adapter 300. Therefore, there is no need to replace optical devices on the optical path or adjust the entire optical path, which is very convenient to use.

[0079] Specifically, the laser 200 is used to emit laser light to the laser adapter 300; the laser adapter 300 is used to receive the laser light emitted by the laser 200, adjust and adapt the laser light, and then transmit the adjusted and adapted laser light to the application device body 100; the application device body 100 is configured to transmit the laser light to the microscope probe 400, and control the microscope probe 400 to perform laser scanning on the living body to generate a fluorescence signal for imaging.

[0080] In the technical solution provided by this application, the laser light generated by the laser 200 passes through the laser adapter 300. The laser adapter 300 can perform transformations such as amplification, reduction, and zooming on the laser beam, converting the various received laser signals into a unified laser signal output, making the laser compatible with subsequently connected devices and facilitating optimal system performance. This allows the use of lasers with various parameters, and even if the distance between the lasers changes, the laser adapter 300 can still output an adapted laser beam to the application device body 100 after transforming the received laser light.

[0081] The optical system provided herein is a multiphoton imaging system, meaning that the microscope probe 400 can employ nonlinear laser scanning imaging, such as two-photon, three-photon, or Raman. In some embodiments, the microscope probe 400 can specifically include a micro-electromechanical system (MEMS) scanning galvanometer and various lenses.

[0082] In one embodiment, the optical system further includes a transmission fiber 301 connected between the laser adapter 300 and the application device body 100. The laser adapter 300 transmits the adjusted and adapted laser light to the application device body 100 through the transmission fiber 301. One end of the transmission fiber 301 can be connected to a laser coupler, which is then connected to the output end of the laser adapter 300, and the other end can be connected to a collimator, which is then connected to the application device body 100.

[0083] The laser adapter 300 is connected to the application device body 100 by optical fiber, so that the application device body 100 can be moved freely. Therefore, the application device body 100 can be placed in different positions as needed, or even placed across platforms, making it more flexible to use.

[0084] Furthermore, the fiber output can shape the beam, making the light spot output from the laser adapter 300 to the application device 100 more uniform, thereby improving system performance. Furthermore, compared to installing a fixed optical path adjustment device between the laser adapter 300 and the application device 100, the fiber connection approach is less susceptible to interference and misoperation, improving system stability. It also reduces the number of optical path adjustment devices installed between modules, making installation and maintenance easier.

[0085] In one embodiment, the application device body 100 includes a laser coupling module 3 , a laser input end 31 of the laser coupling module 3 is connected to a transmission optical fiber 301 , and a laser output end 32 is connected to a microscope probe 400 via a laser transmission optical fiber 401 .

[0086] The laser coupling module 3 is used to adjust the laser light received from the transmission optical fiber 301 and transmit the adjusted laser light to the microscope probe 400 through the laser transmission optical fiber 401. For example, the laser coupling module 3 can perform dispersion compensation and / or intensity adjustment on the laser light.

[0087] Figure 4 is a schematic diagram of an optical path of an optical system according to an embodiment of the present application. As shown in Figure 4 , the laser adapter 300 includes a laser power meter 308 for detecting laser power, and the laser coupling module 3 includes a power detector 311 for detecting laser power.

[0088] The laser power meter 308 can monitor the laser power entering the laser adapter 300 in real time. Power changes detected by the laser power meter 308 can be used to detect abnormalities in laser transmission, typically to determine whether the laser 200 is damaged or the laser is blocked. Specifically, a first beam splitter 309 can be placed in the laser transmission optical path. A portion of the laser beam is split by the first beam splitter 309 and transmitted to the laser power meter 308, which detects the split beam to obtain the laser power. A power detector 311 can monitor the laser power within the laser coupling module 3 in real time. Similarly, by detecting the split beam of the laser beam, the power detector 311 detects the split beam to obtain the laser power. By comparing the power changes detected by the power detector 311 with those detected by the laser power meter 308, it can be determined whether abnormalities in laser transmission between the laser adapter 300 and the application device 100 are present. Therefore, the provision of the laser power meter 308 and the power detector 311 allows for rapid location of any issues in the laser transmission process.

[0089] Optionally, the laser power meter 308 is close to the laser output end 3012 of the laser adapter 300, and the power detector 311 is close to the laser input end 31 of the laser coupling module 3. If the power change of the power detector 311 is larger than that of the laser power meter 308, it can be determined that there is a problem with the laser power meter 308, thereby quickly locating the problem link and achieving rapid maintenance.

[0090] The following describes the laser transmission path based on Figure 4:

[0091] The laser light emitted by the laser 200 enters from the laser input end 3011, is transmitted to the first fixed reflector 302, is deflected approximately 90° by the first fixed reflector 302, is reflected to the second fixed reflector 303, is deflected approximately 90° by the second fixed reflector 303, is transmitted to the beam conversion device 304, is converted by the beam conversion device 304, is transmitted to the first deflecting reflector 305, is deflected approximately 90 degrees by the first deflecting reflector 305, is reflected to the second deflecting reflector 306, and is then reflected to the laser output end 3012 by the second deflecting reflector 306 and coupled into the transmission optical fiber 301 connected to the laser output end 3012.

[0092] The optical fiber transmitted by the transmission optical fiber 301 enters the laser coupling module 3 from the laser input end 31, is dispersion compensated by the dispersion compensation element 34, and is transmitted to the reflector 35. After being deflected approximately 90 degrees by the reflector 35, it is transmitted to the acousto-optic modulator 36. After the acousto-optic modulator 36 adjusts the laser intensity, it is transmitted to the first deflecting reflector 37. After being deflected approximately 90 degrees by the first deflecting reflector 37, it is reflected to the second deflecting reflector 38, and then reflected by the second deflecting reflector 38 to the laser output end 32 and coupled into the laser transmission optical fiber 401 connected to the laser output end 32.

[0093] The optical system provided in the present application may also include a workbench, which includes a workbench host and a display. The workbench host is connected to the application device body 100. The application device body 100 processes the collected fluorescence signal and transmits it to the workbench host, and the display displays the image. The workbench host also sends control instructions to the application device body 100, and then the various control circuits in the control box 5 control the various components.

[0094] The optical system provided herein may further include a behavioral testing apparatus that provides an activity space for a living organism equipped with a microscope probe 400. For example, a mouse equipped with a microscope probe 400 may be placed in the behavioral testing apparatus and allowed to move freely, and the state of its neurons during the free movement of the mouse may be detected.

[0095] On the other hand, one embodiment of the present application provides an application device. Directly recording neuronal activity in freely moving animals is one of the most direct and effective methods for studying the relationship between animal behavior and neural function. Multiphoton microscopy has become the most important and widely used tool for observing animal neurons using fluorescence imaging. Multiphoton microscopy can be a two-photon, three-photon, or Raman nonlinear laser scanning microscope.

[0096] At present, multi-photon microscope equipment has a complex structure and large size, and has problems such as taking up space, having many connections, being difficult to carry and transfer, and having complicated on-site installation and subsequent maintenance.

[0097] The main body of the application device provided in one embodiment of the present application is a multi-photon microscope host 100, which is used to be connected to a microscope probe, and the microscope probe is used to be detachably installed on a living body to observe the neuronal activity of the living body.

[0098] As shown in Figures 5 to 8, the multi-photon microscope host 100 provided in an embodiment of the present application includes a mounting body 1 and a wide-field search module 4, a laser coupling module 3, a fluorescence collection module and a scanning control module integrated on the mounting body 1; wherein, the wide-field search module 4 is configured to perform wide-field imaging of a living body to search for a target area on the living body for installing a microscope probe; the laser coupling module 3 is configured to receive laser light and adjust the laser light to couple the laser light into a laser transmission optical fiber, wherein the laser transmission optical fiber is used to connect the laser coupling module 3 and the microscope probe; the scanning control module is configured to be connected to the microscope probe through a control cable, and is used to control the microscope probe to perform laser scanning on the living body to generate a fluorescence signal; the fluorescence collection module is configured to be connected to the microscope probe through a fluorescence collection optical fiber, and is used to collect the fluorescence signal output by the microscope probe.

[0099] During use, the multiphoton microscope host 100 is connected to a microscope probe 400. For details, see Figures 18 and 19. The laser coupling module 3 of the multiphoton microscope host 100 is connected to the microscope probe 400 via a laser transmission fiber 401. The scanning control module is connected to the microscope probe 400 via a control cable 403. The fluorescence collection module is connected to the microscope probe 400 via a fluorescence collection fiber 402. The microscope probe 400 is designed to be worn on a living body. When the laser coupling module 3 receives laser light and transmits it to the microscope probe 400 via the laser transmission fiber 401, the scanning control module controls the microscope probe 400 via the control cable 403 to scan the living body with the laser light to generate a fluorescence signal. The fluorescence collection module collects the fluorescence signal output by the microscope probe 400 via the fluorescence collection fiber 402. The output fluorescence signal can be converted into an electrical signal and imaged on a computer, which can then be used to observe the neuronal activity of the living body.

[0100] The microscope probe 400 may include a micro-electro-mechanical system (MEMS) scanning galvanometer and various lenses. Therefore, the scanning control module may include a MEMS control module that controls the micro-electro-mechanical system scanning galvanometer.

[0101] The multiphoton microscope host provided in this application integrates various functional modules into an overall structure, which can greatly reduce the occupied space and is suitable for various laboratories. Moreover, it is set as a complete machine, which can make the lines neat and beautiful. In addition, the multiphoton microscope host is small in size and portable, and is easy to carry and change locations. At the same time, the position and direction of the multiphoton microscope can be quickly adjusted according to some experimental requirements, which is convenient for matching more applications. In addition, the multiphoton microscope host is easy to install and maintain quickly on site. Among them, the multiphoton microscope equipment described in this application can be a two-photon, three-photon or Raman nonlinear laser scanning microscope.

[0102] In one embodiment, the multiphoton microscope host 100 also includes a mobile module 7 arranged on the mounting body 1, the mobile module 7 is used to carry the living body and can drive the living body to move in multiple directions, and the wide-field search module 4 is configured to perform a field of view search on the living body located on the mobile module 7.

[0103] Specifically, the living subject can be directly mounted on the mobile module 7, specifically by providing a clamping or limiting structure on the mobile module 7 to restrain the living subject. Alternatively, the living subject can be first mounted on a living subject mounting device 8, which is then secured to the mobile module 7. The mobile module 7 can then be moved with the living subject mounting device 8 to adjust the position of the living subject, thereby allowing the wide-field search module 4 to image different areas of the living subject to search for target locations of interest. The mobile module 7 can be a multi-axis mobile platform capable of moving in multiple directions, including up, down, left, right, forward, and backward.

[0104] The widefield search module 4 is a device capable of imaging a large field of view of a living organism, employing single-photon fluorescence imaging. The images produced by the widefield search module 4 can be transmitted to a computer for display, or an eyepiece can be provided on the widefield search module 4 for direct observation.

[0105] In the embodiment shown in FIG9 , when the wide-field search module 4 is used to perform wide-field imaging on a living body, the living body is mounted on a living body mounting device 8, which is then fixed to a moving module 7. The living body can be a mouse or other animal.

[0106] Figure 10 provides a kind of living body mounting device 8 suitable for mounting mice (not excluding the use for mounting other suitable animals), which includes: a mounting seat 81, a treadmill 88 and a clamping mechanism, and the treadmill 88 and the clamping mechanism are both mounted on the mounting seat 81. The clamping mechanism may include two relatively arranged clamping assemblies 82, which are used to clamp the two sides of the probe mounting member 80 provided on the mouse (the probe mounting member 80 is usually used to be installed on the head of the mouse, and Figure 10 only shows the probe mounting member 80 without showing the mouse). When the clamping mechanism is configured to clamp and fix the probe mounting member 80, the mouse can run on the treadmill 88. Among them, the mounting seat 81 is provided with baffles 811 located on both sides of the treadmill 88 for confining the mouse on the treadmill 88, and the mounting seat 81 is also provided with a fixing frame 812 for fixing on the mobile module 7, which can be fixed by bolts.

[0107] When the clamping assembly 82 is used to clamp the probe mounting member 80 set on the mouse, the mouse runs on the treadmill 88, which can distract the mouse's attention, reduce the stress response of the awake mouse during the installation process, and facilitate rapid experimentation.

[0108] Optionally, each clamping assembly 82 includes two clamping parts and a first adjusting bolt 821. The first adjusting bolt 82 is configured to rotate so that the two clamping parts can move closer together to clamp the probe mounting member 80, or move away from each other to release the probe mounting member 80. Therefore, when clamping or removing the probe mounting member 80, it is only necessary to rotate the first adjusting bolts 821 of the two clamping assemblies 82, making assembly and disassembly convenient.

[0109] Optionally, the two clamping assemblies 82 are configured to be movable relative to the mounting base 81 along the front-rear direction of the treadmill 88 and to be movable up and down relative to the mounting base 81. In this way, the clamping assemblies 82 can be adjusted to a suitable position to clamp the probe mounting member 80 according to the type or size of the living body.

[0110] Specifically, the living body fixation device 8 also includes a movable frame 85 corresponding to each clamping assembly 82. The movable frame 85 is configured to be movable in the front-to-back direction of the treadmill 88 relative to the mounting base 81. The clamping assembly 82 is arranged on the movable frame 85 so as to be liftable. As shown in Figure 6, the movable frame 85 is provided with a second adjustment bolt 86 for adjusting the elevation of the clamping assembly 82. By rotating the second adjustment bolt 86, the second adjustment bolt 86 can lift the clamping assembly 82. By rotating the second adjustment bolt 86 in the opposite direction, the clamping assembly 82 can be lowered under the action of gravity. The mounting base 81 is provided with a third adjustment bolt 87 for adjusting the front-to-back movement of the movable frame 85. The third adjustment screw 87 is threadedly connected to the movable frame 85. Rotating the third adjustment bolt 87 can cause the movable frame 85 to move back and forth relative to the mounting base 81.

[0111] Optionally, the living subject immobilization device 8 further includes a light shielding mechanism for shielding the eyes of the living subject. This light shielding mechanism includes a light shield 83 and a rotating member 84 that drives the light shield 83 to rotate. When performing wide-field search imaging on a mouse, the rotating member 84 can be operated to cause the light shield 83 to shield the mouse's eyes, protecting them from light damage.

[0112] The living body fixing device 8 may further include a water dispenser (not shown) provided on the mounting seat 81 . The water dispenser is configured to provide drinking water for the mice on the treadmill, which can help distract the mice.

[0113] The living body fixing device 8 may further include a receiving tray 89 disposed below the mounting seat 81 for receiving mouse excrement.

[0114] In one embodiment, as shown in Figures 7 and 9, the multiphoton microscope host also includes a field of view search adapter 9 installed on the mounting body 1; the field of view search adapter 9 includes a probe mounting assembly 91 and a switching mechanism, the probe mounting assembly 91 is used to detachably mount the microscope probe 400, and the switching mechanism is configured to be able to switch the probe mounting assembly 91 to a first position and a second position.

[0115] When the probe mounting assembly 91 is in the first position, the microscope probe 400 mounted on the probe mounting assembly 91 avoids the optical path between the wide-field search module 4 and the living body. When the probe mounting assembly 91 is in the second position, the microscope probe 400 is aligned with the optical path of the wide-field search module 4.

[0116] In which, the switching mechanism can be configured to switch the probe mounting assembly 91 between two positions by manual pushing and pulling. Specifically, a gripping portion (not shown in the figure) that is convenient for hand holding can be provided on the switching mechanism. When the probe mounting assembly 91 is pushed by the gripping portion, the probe mounting assembly 91 can be moved to the first position. When the probe mounting assembly 91 is pulled in the opposite direction, the probe mounting assembly 91 can be moved to the second position.

[0117] Furthermore, an objective lens 43 may be provided on the field search adapter 9. When the field search adapter 9 is mounted on the mounting body 1, the objective lens 43 is aligned with the optical path of the widefield search module 4. Furthermore, when the probe mounting assembly 91 is in the second position, the microscope probe 400 mounted thereon needs to be aligned with the optical path of the widefield search module 4. When using the widefield search module 4 for widefield imaging, a switching mechanism is used to switch the probe mounting assembly 91 to the first position. After the widefield search module 4 locates the target area on the living subject, the probe mounting assembly 91 is switched to the second position. The microscope probe 400 is then removed from the probe mounting assembly 91 and secured to the probe mounting component 80 of the living subject at a position corresponding to the searched target area (this may be secured by gluing).

[0118] After the microscope probe 400 is mounted on the probe mounting member 80 , the living body (e.g., a mouse) can be removed from the living body mounting device 8 , releasing the mouse to allow it to move freely, thereby allowing neurons of the freely moving mouse to be observed through the microscope probe 400 .

[0119] In one embodiment, the multiphoton microscope main unit 100 further includes a light-shielding door 2 mounted on the mounting body 1 and capable of being opened and closed. When the light-shielding door 2 is closed, a confined space is formed between the mounting body 1 and the light-shielding door 2, and the mobile module 7 is positioned within the confined space. The wide-field search module 4 is configured to perform a wide-field search of a living organism on the mobile module 7 within the confined space.

[0120] Setting up the light-shielding door 2 can ensure that the living organism is located in a dark room when the wide-field search module 4 images the living organism, thereby achieving a higher imaging signal-to-noise ratio. In addition, setting up the light-shielding door 2 eliminates the need to build a special light-proof environment (such as setting up a large machine cover or turning off the laboratory lights, etc.).

[0121] Alternatively, as shown in Figures 5 and 6, two light-shielding doors 2 may be provided, in a biparting arrangement, i.e., the two light-shielding doors 2 are rotatably mounted on the mounting body 1, and can be closed when rotated toward each other and opened when rotated away from each other. Figure 5 shows the two light-shielding doors 2 in a closed state, and Figure 6 shows the two light-shielding doors 2 in an open state.

[0122] It is understandable that only one light-shielding door 2 may be provided, and the light-shielding door 2 may be opened and closed by lifting or sliding.

[0123] In one embodiment, the specific arrangement of the modules of the multiphoton microscope main unit 100 can be seen in Figures 7 and 8. The mounting body 1 includes a base 11 and a mounting frame 12 fixed to the base 11. A support plate 13 is provided on the upper portion of the mounting frame 12. The mobile module 7 is movably mounted on the base 1 and is located on one side of the mounting frame 12. A control box 5 located below the support plate 13 is fixed to the other side of the mounting frame 12. The scanning control module and the fluorescence collection module are disposed within the control box 5. The laser coupling module 3 is mounted on the support plate 13, and the wide-field search module 4 is mounted above the mobile module 7. A light-shielding door 2 is mounted on the side of the mounting frame 12 facing the mobile module 7 to form a sealed space on the side of the mounting frame 12 with the mobile module 7. The optical path of the wide-field search module 4 located above can enter the sealed space to enable imaging of the living organism on the mobile module 7.

[0124] Optionally, the wide-field search module 4 is mounted on the laser coupling module 3. The laser coupling module 3 is provided with an optical path hole 331 extending vertically therethrough. The optical path of the wide-field search module 4 is arranged to pass downward through the optical path hole 331 to reach the mobile module 7. This arrangement can make the overall structure more compact and smaller in size.

[0125] Optionally, a handle 14 may be provided on the base 11 , and the handle 14 may facilitate the transportation of the multiphoton microscope host 100 .

[0126] Optionally, a display screen 15 may be provided on the base 11 , and the display screen 15 may display, for example, laser parameters, transmission status, temperature and humidity of the multiphoton microscope, etc., to facilitate understanding of the working status of the device.

[0127] In one embodiment, as shown in Figures 11 and 12, the laser coupling module 3 includes a coupler housing 33, a dispersion compensation element 34, an acousto-optic modulator 36, and a beam stabilization device. The dispersion compensation element 34, the acousto-optic modulator 36, and the beam stabilization device are all disposed within the coupler housing 33 and arranged sequentially along the transmission direction of the laser. The dispersion compensation element 34 is used to compensate for the negative dispersion caused by the laser transmission fiber 401 during the laser transmission process; the acousto-optic modulator 36 is used to adjust the intensity of the laser; and the beam stabilization device is used to adjust the laser transmission direction to correct the deviation between the actual position of the laser beam at the output end 32 of the laser coupling module 3 and the ideal position. The beam stabilization device may specifically include a position.

[0128] The position detector 39 is positioned near the output end 32 of the laser coupling module 3 and is used to detect the position information of the laser at the output end 32. The position detector 39 can be a 4D position detector, which can strictly detect and distinguish between the position drift and angle drift of the light beam and accurately detect the real-time position of the light beam. The reflector adjustment mechanism is configured to drive the deflection reflector to deflect according to the position information detected by the position detector 39 to adjust the laser transmission direction, so that the laser is stably output from the output end to the laser transmission fiber, which is beneficial to improving the coupling efficiency.

[0129] Specifically, the ideal position of the laser beam at the output end 32 is first determined. This ideal position is the position at which the ideal coupling efficiency can be achieved when the laser beam is coupled into the connected laser transmission fiber 401 at the output end 32. When the beam deflects, for example, due to vibration or temperature changes that cause the optical device to shift, or due to human touch, the position detector 39 detects the position information of the laser beam at the output end 32 in real time and transmits it to the control unit. The control unit continuously determines the deviation between the position of the laser beam and the ideal position based on this position information, and controls the reflector adjustment mechanism to adjust the deflection reflector, thereby continuously adjusting the reflection direction of the laser beam, so that the laser beam is stably transmitted to the laser transmission fiber 401 within a certain range around the ideal position. The control unit can be disposed within the laser coupler housing 33 or within the control box 5.

[0130] Optionally, the laser coupling module 3 may further include at least one reflector 35 for changing the direction of laser transmission. By changing the direction of laser transmission, the optical path can be bent, facilitating the arrangement of components on the optical path and reducing the volume of the entire laser coupling module.

[0131] As shown in FIG12 , laser light enters the input port 31 of the laser coupling module 3, undergoes dispersion compensation via the dispersion compensation element 34, and is then transmitted to the reflector 35. After being deflected approximately 90 degrees by the reflector 35, the laser light is transmitted to the acousto-optic modulator 36. The acousto-optic modulator 36 adjusts the laser light intensity and then transmits the laser light to the first deflecting reflector 37. After being deflected approximately 90 degrees by the first deflecting reflector 37, the laser light is reflected to the second deflecting reflector 38. The laser light is then reflected by the second deflecting reflector 38 to the output port 32 and coupled into the laser transmission fiber connected to the output port 32. The first deflected reflected laser light 37 and the second deflecting reflector 38 are each equipped with a corresponding reflector adjustment mechanism. The positions of the first deflected reflected laser light 37 and the second deflecting reflector 38 are adjusted in real time based on the position information detected by the position detector 39, ensuring stable laser light output to the laser transmission fiber 401.

[0132] A power detector 311 may also be provided in the laser coupling module 3 for detecting the laser transmission power.

[0133] In addition, as shown in FIG13 , the laser coupling module further includes a driver 361 for driving the AOM 36 and a cooling mechanism for cooling the driver 361. Both the driver 361 and the cooling mechanism are located on the upper surface of the coupler housing 33. The cooling mechanism may include heat dissipation fins 362 for dissipating heat from the driver 361 and a fan 363 for dissipating heat from the heat dissipation fins 362.

[0134] Because the driver 361 drives a large RF power, placing it inside the laser coupler housing 33 increases the risk of interference from high-power RF signals. Moreover, since the driver 361 generates a large amount of heat, it is easy to cause deformation of the precision optical system plate, increase the temperature in the cavity, and affect the performance of the equipment. Therefore, the driver 361 is set outside the coupler housing 33, and heat dissipation fins 362 and fans 363 are added for heat dissipation.

[0135] Optionally, referring to FIG13 , widefield search module 4 is mounted on the upper surface of coupler housing 33. Laser coupling module 3 is provided with an optical through-hole 331 extending vertically therethrough. The optical path of widefield search module 4 is configured to pass downward through optical through-hole 331. Widefield search module 4 may include a fluorescent light source 41 and a camera 42. The optical path of camera 42 passes downward through optical through-hole 331 to reach the living organism on mobile module 7, achieving wide-field imaging of the living organism.

[0136] The multiphoton microscope host may further include a cover 10 for covering the wide-field search module 4 , the driver 361 and the cooling mechanism. The cover 10 not only plays a protective role but also improves the appearance.

[0137] In one embodiment, as shown in FIG. 14 and FIG. 15 , the multiphoton microscope host further includes a control box 5 mounted on the mounting body 1 , and the fluorescence collection module and the scanning control module are both located in the control box 5 .

[0138] The fluorescence collection module 53 may include a photomultiplier tube (PMT), and the signal collected by the fluorescence collection optical fiber 402 is transmitted to the photomultiplier tube.

[0139] Optionally, the fluorescence collection module 53 includes a spectroscope and at least two spectroscopic collection modules, each of which may include a photomultiplier tube. The fluorescence signal collected by the fluorescence collection optical fiber 402 from the microscope probe 400 is split into at least two fluorescence signals by the spectroscope, and then collected by the at least two spectroscopic collection modules.

[0140] The control box 5 may also be provided with a signal processing module configured to process the signal output by the fluorescence acquisition module 53 and transmit the processed signal to a computer for display. For example, the fluorescence signal collected by the fluorescence acquisition module 53 may be converted into an electrical signal, amplified, and then collected and reassembled using high-speed AD acquisition before being transmitted to a computer for display.

[0141] The fluorescence collection module and the signal processing module are both arranged in the control box 5, which shortens the transmission distance of the signal collected by the fluorescence collection module to the signal processing module, reduces the possibility of interference, and improves the reliability of signal transmission.

[0142] Optionally, the control box 5 is provided with a first interface 51 for connecting the control cable 403 and a second interface 52 for connecting the fluorescence collection optical fiber 402 , and the first interface 51 and the second interface 52 are both located at the upper position on the same side of the control box 5 .

[0143] The laser coupling module 3 is located above the control box 5 , and the output end of the laser coupling module 3 for connecting to the laser transmission optical fiber 401 is located on the same side as the first interface 51 and the second interface 52 of the control box 5 .

[0144] By arranging the laser coupling module 3 above the control box 5, the first interface 51 and the second interface 52 of the control box 5 are located above the box body, and the output end of the laser coupling module 3 is located on the same side as the first interface 51 and the second interface 52 of the control box 5, the laser transmission optical fiber 401, the fluorescence collection optical fiber 402 and the control cable 403 can be close to each other, which is conducive to regular and beautiful wiring, and can also enable the various cables to be gathered into a total cable. For example, after being gathered, they can be wrapped into a total cable by a wire sheath, which is convenient for storage in the storage device 6 (the storage of cables and microscope probes by the storage device will be described in detail below).

[0145] Furthermore, the outlets of the laser transmission fiber 401, fluorescence collection fiber 402, and control cable 403 are positioned above the control box 5, facilitating the adaptation of a wider range of behavioral devices and reducing cable length. For example, when a mouse equipped with a microscope probe 400 is placed in a live behavior chamber and allowed to freely move, the cable arrangement facilitates the downward insertion of the microscope probe 400 into the chamber.

[0146] Furthermore, as shown in Figures 14 and 15, a main control circuit board 54 and a fluorescence collection module 53 are provided in the control box 5. The fluorescence collection module 53 is located above the main control circuit board 54, wherein the main control circuit board 54 includes a scanning control module, and may also include a control driving circuit for controlling the laser coupling module 3 (for example, an acousto-optic modulator and a beam stabilization device that controls the laser coupling module 3), a field of view search module 4, and indicator lights, light sensors, temperature and humidity sensors, etc.

[0147] In one embodiment, the multiphoton microscope further includes a storage device 6 , which is mounted on a side of the control box 5 having the first interface 51 and the second interface 52 . Since the laser transmission fiber 401 , the fluorescence collection fiber 402 , and the control cable 403 are all located on this side, the storage device 6 mounted on this side can conveniently accommodate the microscope probe 400 and the cables connected to the microscope probe 400 , including the laser transmission fiber 401 , the fluorescence collection fiber 402 , and the control cable 403 . For convenient storage, the cables can be bundled, for example, by wrapping them in cable sheathing.

[0148] As shown in Figures 16 and 17, the storage device 6 includes: a storage body, on which a winding drum 633 is provided, and an annular space for accommodating the cable is formed around the winding drum 633; ​​a probe bracket 65, which is fixed on the storage body. After the cable is wound on the winding drum 633, the microscope probe 400 can be installed on the probe bracket 65.

[0149] Figure 16 shows the cable wound around the winding drum 633, with the microscope probe 400 mounted on the probe holder 65. By storing the cable and probe in the storage device 6, they are protected from being easily touched or pressed, which could cause damage. This also prevents the cables from becoming tangled or damaged by random placement, or from being bent and bent. Furthermore, storing the cables and probe in the storage device 6 creates a neater and more aesthetically pleasing appearance, improving visual quality.

[0150] Specifically, the storage body may include: a storage box 61 and a cable retaining drum 63 fixed to the storage box 61, and a probe bracket 65 fixed to the outer side of the cable retaining drum 63 facing away from the storage box 61. The winding drum 633 is provided on one of the storage box 61 and the cable retaining drum 62, and the storage box 61 and the cable retaining drum 63 are configured to stop the cable wound on the winding drum 633 from both ends of the winding drum 633.

[0151] In the embodiment shown in Figure 17, the wire retaining drum 63 includes a winding drum 633 and a wire retaining ring 632 that radially protrudes from the winding drum 633. When the winding drum 633 is fixed on the receiving box 61, an annular space surrounding the winding drum 633 is formed between the wire retaining ring 632 and the receiving box 61. Of course, the winding drum 633 can also be directly formed on the receiving box 61, and the wire retaining drum 63 is fixed to the end of the winding drum 633 that is away from the receiving box 61 and has the wire retaining ring 632 that radially protrudes from the winding drum 633.

[0152] Among them, the probe bracket 65 can be of various structural forms, as long as it can install the microscope probe 400. For example, the probe bracket 65 can be set as a clamping structure that can clamp the probe, or a socket can be set on the probe bracket 65 to insert the microscope probe 400 into the socket.

[0153] Optionally, a plurality of limiting notches are arranged circumferentially on the outer circumference of the wire retaining drum 63 (i.e., on the wire retaining ring 632), and the cable extends from the winding drum 633 to the outside of the wire retaining drum 63 so that when the probe is installed on the probe bracket 65, the cable is limited by one of the limiting notches.

[0154] Optionally, a wire clamping ring 64 may be provided on the outer side of the wire retaining disc 63, facing away from the winding drum 633. The wire clamping ring 64 is provided with a plurality of slots 643 arranged around the probe holder 65. The wire clamping ring 64 may be provided with a plurality of protrusions spaced circumferentially, with slots 643 formed between adjacent protrusions. The cable extends to the outer side of the wire retaining disc 63 so that when the probe is mounted on the probe holder 65, it can be stuck in one of the slots 643. This prevents the cable from loosening from the winding drum 633 and also prevents the probe from being driven off the probe holder due to swinging or loosening of the cable. The wire clamping ring 64 may be made of a flexible material, such as a rubber material. The elasticity of the flexible material allows the cable to be easily inserted into and removed from the slot.

[0155] In one embodiment, a through hole 612 is formed on the side of the storage box 61 for abutting against the control box 5, and the through hole 612 is connected to the annular space for accommodating the cable. One end of the cable with the microscope probe 400 enters the storage box 61 from the through hole 612 and can extend into the annular space and be wound on the winding drum 633.

[0156] Specifically, the storage box 61 is provided with a protrusion 611 surrounding the through hole 612, protruding toward the winding drum 633. An annular groove 613 is formed on the radially outer side of the protrusion 611 away from the through hole 612. The cable retaining drum 63 is provided with a winding drum 633, which is fixed on the protrusion 611. The annular groove 613 forms an annular space for winding the cable between the storage box 61 and the cable retaining drum 63. The protrusion 611 can be provided as an annular structure or an arc-shaped structure with a notch. To install the winding drum 633, an external thread can be provided on the outer surface of the protrusion 611, and an internal thread can be provided on the inner surface of the winding drum 633. The winding drum 633 can be connected to the protrusion 611 of the storage box 61 by a threaded connection.

[0157] The protrusion 611 is provided with a through-groove 614 that radially extends through the protrusion 611. After the end of the cable, which includes the microscope probe 400, enters the storage box 61 through the through-hole 612, it can extend outward from the through-groove 614 into the annular space. The through-groove 614 can be a notch formed in the protrusion 611, as shown in FIG13 , or a through-hole formed in the wall of the protrusion 611. When the winding drum 633 is secured on the protrusion 611, the through-groove 614 is approximately located at one end of the winding drum 633, allowing the cable to extend from the through-groove 614 into the annular space and be wound around the winding drum 633.

[0158] In one embodiment, the storage device further includes a ring indicator light 62, which is mounted on the storage body and is arranged around the center of the winding drum 633. The ring indicator light 62 can be configured to illuminate the interior of the storage device 6, or can be configured to indicate the working status of the multiphoton microscope host. For example, the ring indicator light 62 can be configured to use different colors to indicate whether the device is in a working state, a non-working state, a fault, or an abnormality. For example, when it is detected that the laser enters the laser coupling module 3, or when it is detected that the laser emits laser light, the controller can control the ring indicator light 62 to display green, indicating that the device is in a working state; when it detects a device abnormality, such as abnormal laser power, or other abnormal conditions, the controller can control the ring indicator light 62 to display red for warning; and when the device is in a non-working state, the ring indicator light 62 can display yellow for internal lighting.

[0159] The wire retaining disc 63 includes a light-transmitting cover 631, and the annular indicator light 62 is arranged between the storage box 61 and the wire retaining disc 63 and corresponds to the light-transmitting cover 631. The light of the annular indicator light 62 can be seen through the light-transmitting cover 631. The wire retaining ring 632 of the wire retaining disc 63 is arranged on the outer ring of the light-transmitting cover 631.

[0160] In addition, the storage device 6 may further include a protective cover 66 covering the outside of the storage body. The protective cover 66 is configured to be rotatably mounted on the storage body via a rotating shaft 67 and a hinge 68, and can be rotated between an open state and a closed state. To maintain the protective cover 66 in the closed state, a magnet may be provided between the protective cover 66 and the storage body.

[0161] The protective cover 6 may include an annular cover body 661 and a transparent observation window 62 arranged in the center of the annular cover body 661 to facilitate observation of the internal situation of the storage device and the device status indicated by the annular indicator light 62.

[0162] When in use, the protective cover 66 is rotated to open, the microscope probe 400 is removed from the probe holder 65, and the cable is unwound from the winding drum 633, and the probe can be installed on a living body for use.

[0163] According to another aspect of the present application, an optical system is also provided, as shown in Figures 18 and 19, the multiphoton microscope system includes: a laser 200, a microscope probe 400 and the multiphoton microscope host 100 as described above; the laser 200 is configured to transmit laser light to a laser coupling module 3, the output end of the laser coupling module 3 is connected to the microscope probe 400 through a laser transmission optical fiber 401, the fluorescence collection module is connected to the microscope probe 400 through a fluorescence collection optical fiber 402, and the scanning control module is connected to the microscope probe 400 through a control cable 403.

[0164] In one embodiment, as shown in FIG18 , the optical system further includes a laser adapter 300 . The laser 200 first emits laser light to the laser adapter 300 . The laser light is adjusted and adapted by the laser adapter 300 and then transmitted to the multiphoton microscope host 100 through the transmission optical fiber 301 .

[0165] By placing a laser adapter 300 between the laser 200 and the multiphoton microscope mainframe 100, lasers with different parameters emitted by various lasers 200 can be adjusted by the laser adapter 300 to adapt to the subsequent device. Furthermore, because the laser adapter 300 and the multiphoton microscope mainframe 100 are connected via a transmission optical fiber 301, the multiphoton microscope 100 can be freely moved and can be placed in different locations as needed, even across platforms, making it more flexible.

[0166] The laser adapter 300 may specifically include a housing and a beam conversion device and a beam stabilization device located within the housing. The beam conversion device is configured to convert the laser beam entering the housing. Beam conversion refers to magnifying, reducing, and zooming the beam through the conversion characteristics of optical elements, so that the laser can match the subsequent equipment to achieve optimal performance of the equipment. The beam stabilization device is located downstream of the beam conversion device along the laser transmission direction and is used to adjust the deflection direction of the laser beam to correct the deviation between the actual position of the laser beam at the laser output port and the ideal position. The beam stabilization device is similar to the configuration of the beam conversion device in the laser coupling module 3. The beam stabilization device can adjust the deflection direction of the laser when a deviation of the laser beam is detected, so that the laser output is stable, thereby ensuring the coupling efficiency of the laser output.

[0167] In the embodiment shown in FIG19 , a fixed optical path can be set between the laser 200 and the multiphoton microscope host 100 . However, in this case, there cannot be movement between the laser 200 and the multiphoton microscope host 100 . If the position needs to be changed, the optical path needs to be rebuilt.

[0168] The above description is only a preferred embodiment of the present application and is not intended to limit the present application. Any modifications, equivalent replacements, etc. made within the spirit and principles of the present application shall be included in the scope of protection of the present application.

Claims

1. A laser adapter, comprising: A housing and a beam conversion device and a beam stabilization device arranged in the housing; wherein, The housing has a laser input port and a laser output port; The beam transformation device is configured to transform the laser beam entering the housing; The beam stabilizing device is arranged downstream of the beam transforming device along the laser transmission direction, and is used to adjust the laser transmission direction to correct the deviation between the actual position of the laser beam at the laser output port and the ideal position.

2. The laser adapter according to claim 1, wherein: The beam stabilization device includes: a position detector, at least one deflecting reflector, and a reflector adjustment mechanism connected to each of the deflecting reflector; The position detector is arranged near the laser output port and is used to detect the position information of the laser at the laser output port; The reflector adjustment mechanism is configured to drive the deflection reflector to deflect according to the position information detected by the position detector to adjust the laser transmission direction.

3. The laser adapter according to claim 2, wherein: The laser adapter further includes a control unit, which receives position information detected by the position detector and controls the reflector adjustment mechanism according to the position information.

4. The laser adapter according to claim 2 or 3, wherein: The laser adapter further comprises at least one fixed reflector for changing the transmission direction of the laser light, wherein the fixed reflector is arranged upstream of the beam transforming device along the transmission direction of the laser light.

5. The laser adapter according to claim 4, wherein: At least one of the fixed reflective mirrors includes a first fixed reflective mirror and a second fixed reflective mirror, and at least one of the deflecting reflective mirrors includes a first deflecting reflective mirror and a second deflecting reflective mirror; The laser light is reflected by the first fixed reflector to the second fixed reflector, and the second fixed reflector reflects the laser light to the beam conversion device; The laser light emitted by the beam conversion device is reflected by the first deflecting reflector to the second deflecting reflector, and the second deflecting reflector is configured to reflect the laser light to the laser output port.

6. The laser adapter according to any one of claims 1 to 5, wherein: At least one laser power meter is provided in the housing for detecting the power of the laser entering the laser adapter.

7. The laser adapter according to claim 6, wherein: The at least one laser power meter includes a first laser power meter and a second laser power meter. The first laser power meter is arranged near the laser input port, and the second laser power meter is arranged near the laser output port.

8. The laser adapter according to any one of claims 1 to 7, wherein: The laser adapter further comprises a switch device provided at the laser input port, the switch device comprising a switch door for opening and closing the laser input port and a door driving mechanism for driving the switch door to switch between an open state and a closed state; When the switch door is open, the laser can enter the laser adapter for transmission; when the switch door is closed, the laser is blocked from entering the laser adapter.

9. The laser adapter according to any one of claims 1 to 8, wherein: Support legs are provided below the shell, and the support legs are arranged to be height-adjustable.

10. An optical system comprising a laser, an application device body, and a laser adapter according to any one of claims 1 to 9; wherein, The laser is used to emit laser light to the laser adapter; The laser adapter is used to receive the laser light emitted by the laser device, adjust and adapt the laser light, and then transmit the adjusted and adapted laser light to the application device body; The application device body is configured to transmit laser light to a microscope probe and control the microscope probe to Laser scanning is performed on the living body to generate fluorescent signals for imaging.

11. The optical system according to claim 10, wherein: The optical system further includes a transmission optical fiber connected between the laser adapter and the application device body, and the laser adapter transmits the adjusted and adapted laser light to the application device body through the transmission optical fiber.

12. The optical system according to claim 11, wherein the application device body comprises a laser coupling module, wherein a laser input end of the laser coupling module is connected to the transmission optical fiber, and a laser output end of the laser coupling module is connected to the microscope probe via a laser transmission optical fiber; The laser coupling module is used to adjust and process the laser light received from the transmission optical fiber and then transmit the adjusted laser light to the microscope probe through the laser transmission optical fiber.

13. The optical system according to claim 12, wherein the laser coupling module comprises a power detector for detecting laser power.

14. The optical system according to claim 13, wherein: The laser power meter is close to the laser output end of the laser adapter, and the power detector is close to the laser input end of the laser coupling module.

15. The optical system according to any one of claims 10 to 14, wherein: The application device body includes a multiphoton microscope host, wherein: The multiphoton microscope host is used to connect with the microscope probe, including a mounting body and a wide-field search module, a laser coupling module, a fluorescence collection module and a scanning control module integrated on the mounting body; wherein, The wide-field search module is configured to perform wide-field imaging of a living body, so as to search for a target area for installing a microscope probe on the living body; The laser coupling module is configured to receive laser light and adjust the laser light so as to couple the laser light into a laser transmission optical fiber, wherein the laser transmission optical fiber is used to connect the laser coupling module and the microscope probe; The scanning control module is configured to be connected to the microscope probe via a control cable, and is used to control the microscope probe to perform laser scanning to generate a fluorescence signal; The fluorescence collection module is configured to be connected to the microscope probe via a fluorescence collection optical fiber, and is used to collect the fluorescence signal output by the microscope probe.

16. The optical system according to claim 15, wherein: The multiphoton microscope further includes a field search adapter mounted on the mounting body; The field search adapter includes a probe mounting assembly and a switching mechanism, wherein the probe mounting assembly is used to detachably mount the microscope probe, and the switching mechanism is configured to switch the probe mounting assembly to a first position and a second position; When the probe mounting assembly is in the first position, the microscope probe mounted on the probe mounting assembly avoids the optical path between the wide-field search module and the living body; when the probe mounting assembly is in the second position, the microscope probe is aligned with the optical path of the wide-field search module.

17. The optical system according to claim 15 or 16, wherein: The multiphoton microscope host also includes a moving module arranged on the mounting body, the moving module is used to carry the living body and can drive the living body to move in multiple directions, and the wide-field search module is configured to perform a field of view search for the living body located on the moving module.

18. The optical system according to claim 17, wherein: The multiphoton microscope host further includes a light shielding door installed on the installation body and capable of being opened and closed; When the light-shielding door is in a closed state, a closed space is formed between the installation body and the light-shielding door, and the mobile module is located in the closed space; The wide-field search module is configured to perform a wide-field search on a living body on the mobile module within a confined space.

19. The optical system according to claim 17 or 18, wherein: The installation body includes a base and a mounting frame fixed on the base, and a support plate is provided on the upper portion of the mounting frame; wherein, The mobile module is movably mounted on the base and is located on one side of the mounting frame. A control box is installed below the support plate on the other side, and the scanning control module and the fluorescence collection module are arranged in the control box; The laser coupling module is installed above the supporting plate, and the wide-field search module is installed above the moving module.

20. The optical system according to claim 18 or 19, wherein: The wide-field search module is mounted on the laser coupling module. The laser coupling module is provided with an optical path through-hole running vertically. The optical path of the wide-field search module is configured to pass downward through the optical path through-hole to reach the moving module.

21. The optical system according to any one of claims 15 to 20, wherein: The laser coupling module includes a coupler housing, a dispersion compensation element, an acousto-optic modulator, and a beam stabilization device, wherein the dispersion compensation element, the acousto-optic modulator, and the beam stabilization device are all arranged in the coupler housing and arranged in sequence along the transmission direction of the laser; wherein, The dispersion compensation element is used to compensate for the negative dispersion caused by the transmission optical fiber during the transmission of the laser; The acousto-optic modulator is used to adjust the intensity of the laser; The beam stabilization device is used to adjust the laser transmission direction to correct the deviation between the actual position of the laser beam at the laser output end of the laser coupling module and the ideal position.

22. The optical system according to claim 21, wherein The laser coupling module further includes a driver for driving the acousto-optic modulator and a cooling mechanism for cooling the driver. Both the driver and the cooling mechanism are located on the upper surface of the coupler housing.

23. The optical system according to claim 22, wherein: The wide-field search module is mounted on the upper surface of the coupler housing, the laser coupling module is provided with an optical path through-hole running vertically, and the optical path of the wide-field search module is arranged to pass downward through the optical path through-hole; The multiphoton microscope host further includes a cover for covering the wide-field search module, the driver, and the cooling mechanism.

24. The optical system according to any one of claims 15 to 23, wherein: The multiphoton microscope host further includes a control box mounted on the mounting body, and the fluorescence collection module and the scanning control module are both located in the control box.

25. The optical system of claim 24, wherein: The control box is provided with a first interface for connecting the fluorescence collection optical fiber and a second interface for connecting the control cable, and the first interface and the second interface are both located at an upper position on the same side of the control box; The laser coupling module is located above the control box, and an output end of the laser coupling module for connecting to the laser transmission optical fiber is located on the same side as the first interface and the second interface of the control box.

26. The optical system according to claim 25, wherein the first interface and the second interface are provided on one side; The storage device is used to store the microscope probe and the cables connected to the microscope probe, including the laser transmission optical fiber, the fluorescence collection optical fiber and the control cable.

27. The optical system according to any one of claims 10 to 26, wherein: Also included is a workbench, which includes a workbench host and a display; The workbench host is connected to the microscope host, the microscope host processes the collected fluorescence signal and transmits it to the workbench host, and the display shows the image; The workbench host also sends control instructions to the microscope host.

28. A multiphoton microscope host, used to connect to a microscope probe, the multiphoton microscope host comprising a mounting body and a wide-field search module, a laser coupling module, a fluorescence collection module, and a scanning control module integrated on the mounting body; wherein: The wide-field search module is configured to perform wide-field imaging of a living body, so as to search for a target area for installing a microscope probe on the living body; The laser coupling module is configured to receive laser light and adjust the laser light so as to couple the laser light into a laser transmission optical fiber, wherein the laser transmission optical fiber is used to connect the laser coupling module and the microscope probe; The scanning control module is configured to be connected to the microscope probe via a control cable, and is used to control the microscope probe to perform laser scanning to generate a fluorescence signal; The fluorescence collection module is configured to be connected to the microscope probe via a fluorescence collection optical fiber, and is used to collect the fluorescence signal output by the microscope probe.

29. The multiphoton microscope host according to claim 28, wherein: The multiphoton microscope further includes a field search adapter mounted on the mounting body; The field search adapter includes a probe mounting assembly and a switching mechanism, wherein the probe mounting assembly is used to detachably mount the microscope probe, and the switching mechanism is configured to switch the probe mounting assembly to a first position and a second position; When the probe mounting assembly is in the first position, the microscope probe mounted on the probe mounting assembly avoids the optical path between the wide-field search module and the living body; when the probe mounting assembly is in the second position, the microscope probe is aligned with the optical path of the wide-field search module.

30. The multiphoton microscope host according to claim 28 or 29, wherein: The multiphoton microscope host also includes a moving module arranged on the mounting body, the moving module is used to carry the living body and can drive the living body to move in multiple directions, and the wide-field search module is configured to perform a field of view search for the living body located on the moving module.

31. The multiphoton microscope host according to claim 30, wherein: The multiphoton microscope host further includes a light shielding door installed on the installation body and capable of being opened and closed; When the light-shielding door is in a closed state, a closed space is formed between the installation body and the light-shielding door, and the mobile module is located in the closed space; The wide-field search module is configured to perform a wide-field search on a living body on the mobile module within a confined space.

32. The multiphoton microscope host according to claim 30 or 31, wherein: The installation body includes a base and a mounting frame fixed on the base, and a support plate is provided on the upper portion of the mounting frame; wherein, The mobile module is movably mounted on the base and is located on one side of the mounting frame. A control box located below the support plate is mounted on the other side of the mounting frame. The scanning control module and the fluorescence collection module are arranged in the control box. The laser coupling module is installed above the supporting plate, and the wide-field search module is installed above the moving module.

33. The multiphoton microscope host according to claim 31 or 32, wherein: The wide-field search module is mounted on the laser coupling module. The laser coupling module is provided with an optical path through-hole running vertically. The optical path of the wide-field search module is configured to pass downward through the optical path through-hole to reach the moving module.

34. The multiphoton microscope mainframe according to any one of claims 28 to 33, wherein: The laser coupling module includes a coupler housing, a dispersion compensation element, an acousto-optic modulator, and a beam stabilization device, wherein the dispersion compensation element, the acousto-optic modulator, and the beam stabilization device are all arranged in the coupler housing and arranged in sequence along the transmission direction of the laser; wherein, The dispersion compensation element is used to compensate for the negative dispersion caused by the transmission optical fiber during the transmission of the laser; The acousto-optic modulator is used to adjust the intensity of the laser; The beam stabilization device is used to adjust the laser transmission direction to correct the deviation between the actual position of the laser beam at the laser output end of the laser coupling module and the ideal position.

35. The multiphoton microscope host according to claim 34, wherein: The laser coupling module further includes a driver for driving the acousto-optic modulator and a cooling mechanism for cooling the driver. Both the driver and the cooling mechanism are located on the upper surface of the coupler housing.

36. The multiphoton microscope mainframe according to claim 35, wherein: The wide-field search module is mounted on the upper surface of the coupler housing, the laser coupling module is provided with an optical path through-hole running vertically, and the optical path of the wide-field search module is arranged to pass downward through the optical path through-hole; The multiphoton microscope host further includes a cover for covering the wide-field search module, the driver, and the cooling mechanism.

37. The multiphoton microscope mainframe according to any one of claims 28 to 36, wherein: The multiphoton microscope host further includes a control box mounted on the mounting body, and the fluorescence collection module and the scanning control module are both located in the control box.

38. The multiphoton microscope host according to claim 37, wherein: The control box is provided with a first interface for connecting the fluorescence collection optical fiber and a second interface for connecting the control cable, and the first interface and the second interface are both located at an upper position on the same side of the control box; The laser coupling module is located above the control box, and an output end of the laser coupling module for connecting to the laser transmission optical fiber is located on the same side as the first interface and the second interface of the control box.

39. The multiphoton microscope mainframe according to claim 38, wherein: The multiphoton microscope further includes a storage device, which is installed on a side of the control box having the first interface and the second interface; The storage device is used to store the microscope probe and the cables connected to the microscope probe, including the laser transmission optical fiber, the fluorescence collection optical fiber and the control cable.

40. A multiphoton microscope system, comprising: A laser, a microscope probe and a multiphoton microscope host according to any one of claims 28 to 39; The laser is configured to transmit laser light to the laser coupling module, the output end of the laser coupling module is connected to the microscope probe via a laser transmission optical fiber, the fluorescence collection module is connected to the microscope probe via a fluorescence collection optical fiber, and the scanning control module is connected to the microscope probe via a control cable.

41. An optical imaging system comprising: Laser, laser adapter and microscope host; among which, The laser is used to emit laser light to the laser adapter; The laser adapter is used to receive the laser light emitted by the laser device, adjust and adapt the laser light, and then transmit the adjusted and adapted laser light to the microscope host; The microscope host is configured to transmit laser light to a microscope probe, and control the microscope probe to perform laser scanning on a living body to generate a fluorescent signal for imaging.