Carbohydrate combustion promoter

Abstract

To provide a carbohydrate combustion promoter that exhibits superior carbohydrate combustion promoting effects.SOLUTION: A carbohydrate combustion promoter comprises oil and fat with diacylglycerol comprising at least 20 mass% eicosapentaenoic acid in its constituent fatty acids.SELECTED DRAWING: None

Description

[Technical field]

[0001] The present invention relates to a carbohydrate burning accelerator. [Background technology]

[0002] In recent years, "carbohydrate-restricted diets" that reduce carbohydrate intake have been attracting attention, and in order to prevent metabolic syndrome including obesity, the burning of not only lipids but also carbohydrates in the body is an important factor. Exercise is an effective way to promote the burning of carbohydrates. Exercise increases the metabolism of the whole body and increases energy consumption. However, exercise is a heavy burden and not everyone can easily do it. Therefore, there is a demand for carbohydrate burning promoters that can be used easily and daily and have few side effects.

[0003] Eicosapentaenoic acid (C20:5, EPA) is a linear highly unsaturated fatty acid with 20 carbon atoms and five double bonds, and has been reported to have physiological effects such as anti-inflammatory effects and platelet aggregation inhibitory effects. It has also been reported that oral intake of ethyl esters of EPA and docosahexaenoic acid (C22:6, DHA) promotes lipid utilization (Non-Patent Document 1). On the other hand, it has been reported that their effect on carbohydrate utilization is conversely suppressed (Non-Patent Document 1). Furthermore, it has been reported that oral ingestion of EPA esters and DHA esters between meals suppresses the rise in blood glucose level after sugar loading (Patent Document 1), but the effect is limited to after meals.

[0004] On the other hand, it has been reported that fats and oils containing 5% by weight or more of diglycerides and / or monoglycerides whose constituent acyl groups have an ω3 unsaturated acyl group content of 15% by weight or more have the effect of lowering fasting blood glucose levels and blood insulin concentrations (Patent Documents 2 and 3). However, in the above-mentioned Patent Documents 2 and 3, the ω3 unsaturated acyl group is mainly a docosahexaenoyl group or an α-linoleyl group. There have been no reports so far on the effect of diacylglycerol-containing fats and oils containing a high concentration of eicosapentaenoic acid on carbohydrate burning. [Prior art documents] [Patent documents]

[0005] [Patent Document 1] International Publication No. 2012 / 063820 [Patent Document 2] JP 2001-247457 A [Patent Document 3] JP 2001-247473 A [Non-patent literature]

[0006] [Non-Patent Document 1] Charlotte J Green et al., Hepatic de novo lipogenesis is suppressed and fat oxidation is increased by omega-3 fatty acids at the expense of glucose metabolism, BMJ Open Diabetes Res Care., 2020, Vol. 8, No. 1, e000871 Summary of the Invention [Problem to be solved by the invention]

[0007] The present invention relates to providing a carbohydrate combustion promoter that exhibits an excellent carbohydrate combustion promoting effect. [Means for solving the problem]

[0008] In view of the above problems, the inventors conducted extensive research and found that carbohydrate burning was significantly increased by continuously feeding mice fats and oils containing diacylglycerol with a high concentration of eicosapentaenoic acid.

[0009] That is, the present invention provides a carbohydrate burning accelerator comprising, as an active ingredient, an oil or fat containing diacylglycerol in which 20% by mass or more of the constituent fatty acids are eicosapentaenoic acid. Effect of the Invention

[0010] The carbohydrate burning promoter of the present invention constantly exerts an excellent carbohydrate burning promoting effect, and is therefore useful for preventing, improving or treating obesity, diabetes and metabolic syndrome. [Brief description of the drawings]

[0011] [Figure 1] This shows the amount of carbohydrates burned during the light period. [Diagram 2] This shows the amount of carbohydrates burned during the dark period. [Diagram 3] This shows the amount of carbohydrates burned overall (light and dark periods). DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0012] In the present invention, the term "oil and fat" refers to an ester compound of a fatty acid and glycerin, and includes at least one of monoacylglycerol, diacylglycerol, and triacylglycerol. There is no particular limitation on the type of oil and fat, and any type that can be used as an edible oil and fat may be used.

[0013] The fats and oils used in the present invention are fats and oils containing diacylglycerols in which 20% by mass or more of the constituent fatty acids are eicosapentaenoic acid. Hereinafter, in this specification, "fat and oils containing diacylglycerols in which 20% by mass or more of the constituent fatty acids are eicosapentaenoic acid" may be simply referred to as "fat and oils of the present invention."

[0014] In the present invention, the content of eicosapentaenoic acid in the fatty acid constituting diacylglycerol is 20% by mass or more. The content of eicosapentaenoic acid in the fatty acid constituting diacylglycerol is 20% by mass or more, preferably 25% by mass or more, more preferably 27% by mass or more, and even more preferably 30% by mass or more, from the viewpoint of promoting carbohydrate combustion, and is preferably 99% by mass or less, more preferably 98% by mass or less, and even more preferably 95% by mass or less, from the viewpoint of oxidation stability. The content of eicosapentaenoic acid in the fatty acid constituting diacylglycerol is preferably 20% by mass or more and 99% by mass or less, more preferably 25% by mass or more and 98% by mass or less, more preferably 27% by mass or more and 98% by mass or less, and even more preferably 30% by mass or more and 95% by mass or less. In this specification, the amount of fatty acid is calculated as the amount of free fatty acid.

[0015] The constituent fatty acid other than eicosapentaenoic acid constituting the diacylglycerol is not particularly limited, and may be either a saturated fatty acid or an unsaturated fatty acid. From the viewpoints of flavor and industrial productivity of the oil and fat, the content of unsaturated fatty acids in the fatty acids constituting the diacylglycerol is preferably 60 to 100% by mass, more preferably 70 to 99.8% by mass, and even more preferably 80 to 99.5% by mass. From the viewpoint of physiological effects, the number of carbon atoms of the unsaturated fatty acids is preferably 14 to 24, and more preferably 16 to 22.

[0016] In particular, the content of linoleic acid (C18:2) in the fatty acids constituting diacylglycerol is, from the viewpoint of industrial productivity of fats and oils, preferably 0.4 mass% or more, more preferably 0.5 mass% or more, even more preferably 1 mass% or more, and even more preferably 1.2 mass% or more, and is preferably 30 mass% or less, more preferably 20 mass% or less, and even more preferably 10 mass% or less.

[0017] Furthermore, the content of oleic acid (C18:1) in the fatty acids constituting the diacylglycerol is, from the viewpoint of industrial productivity of the oil or fat, preferably 1 mass% or more, more preferably 5 mass% or more, even more preferably 7 mass% or more, and is preferably 30 mass% or less, more preferably 25 mass% or less, even more preferably 20 mass% or less.

[0018] From the viewpoint of industrial productivity of fats and oils, the content of docosahexaenoic acid (C22:6) in the fatty acids constituting the diacylglycerol is preferably 0.3% by mass or more, more preferably 3% by mass or more, even more preferably 4% by mass or more, even more preferably 5% by mass or more, and is preferably 30% by mass or less, more preferably 27% by mass or less, even more preferably 25% by mass or less, even more preferably 20% by mass or less. The content of docosahexaenoic acid in the fatty acids constituting the diacylglycerol is preferably 30% by mass or less, more preferably 0.3 to 30% by mass, even more preferably 3 to 27% by mass, even more preferably 4 to 25% by mass, even more preferably 5 to 20% by mass.

[0019] The total content of saturated fatty acids in the fatty acids constituting the diacylglycerol is preferably 20% by mass or less, more preferably 15% by mass or less, even more preferably 13% by mass or less, and preferably 0.5% by mass or more, from the viewpoints of appearance, physiological effects, and industrial productivity of the oil and fat. The total content of saturated fatty acids in the fatty acids constituting the diacylglycerol is preferably 0 to 20% by mass, more preferably 0.5 to 15% by mass, and even more preferably 0.5 to 13% by mass. In this specification, the saturated fatty acid preferably has 14-24 carbon atoms, and more preferably 14-22 carbon atoms.

[0020] The content of diacylglycerol in the fat of the present invention is not particularly limited, but from the viewpoint of effectively expressing the effect and from the viewpoint of physiological effect, it is preferably 40% by mass or more, more preferably 50% by mass or more, even more preferably 60% by mass or more, even more preferably 65% ​​by mass or more, and from the viewpoint of industrial productivity, it is preferably 99.5% by mass or less, more preferably 98% by mass or less, even more preferably 95% by mass or less, even more preferably 90% by mass or less. The content of diacylglycerol in the fat of the present invention is preferably 40% by mass or more, more preferably 40 to 99.5% by mass, even more preferably 50 to 98% by mass, even more preferably 60 to 95% by mass, even more preferably 65 to 90% by mass.

[0021] The fat or oil of the present invention may contain triacylglycerol, and the content thereof is, from the viewpoint of industrial productivity of the fat or oil, preferably 1% by mass or more, more preferably 2% by mass or more, even more preferably 5% by mass or more, even more preferably 10% by mass or more, and is preferably 85% by mass or less, more preferably 80% by mass or less, even more preferably 75% by mass or less, even more preferably 50% by mass or less, even more preferably 40% by mass or less.

[0022] The content of monoacylglycerol in the fat or oil of the present invention is preferably 5% by mass or less, more preferably 3% by mass or less, even more preferably 2% by mass or less, even more preferably 1.5% by mass or less, and preferably more than 0% by mass, from the viewpoints of flavor, industrial productivity of the fat or oil, and oxidation stability. The content of monoacylglycerol in the fat or oil may be 0% by mass.

[0023] The fats and oils of the present invention may contain free fatty acids or salts thereof as impurities. The content of free fatty acids or salts thereof in the fats and oils is preferably 3% by mass or less, more preferably 2% by mass or less, even more preferably 1% by mass or less, and also preferably more than 0% by mass, from the viewpoints of flavor and oxidation stability. The content of free fatty acids or salts thereof in the fats and oils may be 0% by mass.

[0024] In the present invention, the fatty acids constituting the oil or fat may be the same as or different from the fatty acids constituting the diacylglycerol described above, but from the viewpoint of effectively exerting the effect, it is preferable that they are the same. The content of eicosapentaenoic acid in the fatty acids constituting the fats and oils is preferably 20 to 99 mass %, more preferably 20 to 98 mass %, even more preferably 25 to 97 mass %, and still more preferably 30 to 95 mass %. The content of unsaturated fatty acids in the fatty acids constituting the fats and oils is preferably 60 to 100% by mass, more preferably 70 to 99.8% by mass, and even more preferably 80 to 99.5% by mass. The content of docosahexaenoic acid in the fatty acids constituting the fats and oils is preferably 30% by mass or less, more preferably 0.3 to 30% by mass, even more preferably 3 to 27% by mass, even more preferably 4 to 25% by mass, and even more preferably 5 to 20% by mass.

[0025] The total content of saturated fatty acids in the fatty acids constituting the fat or oil is preferably 0 to 20 mass %, more preferably 0.5 to 17 mass %, and even more preferably 0.5 to 15 mass %.

[0026] The fat or oil of the present invention can be obtained by an esterification reaction between fatty acids derived from fat or oil and glycerin, or an ester exchange reaction (glycerolysis) between fat or oil and glycerin. The esterification reaction and the glycerolysis reaction are roughly classified into a chemical method using a chemical catalyst such as an alkali metal or its alloy, an oxide or hydroxide of an alkali metal or an alkaline earth metal, or an alkoxide of an alkali metal or an alkaline earth metal, and an enzymatic method using an enzyme such as lipase. Among these, from the viewpoint of controlling the fatty acid composition, an esterification reaction between fractionated fatty acids derived from fats and oils, which will be described later, and glycerin is preferred.

[0027] In the present invention, the fats and oils (edible fats and oils) may be either vegetable fats and oils or animal fats and oils. For example, vegetable fats and oils such as soybean oil, rapeseed oil, safflower oil, rice oil, corn oil, sunflower oil, cottonseed oil, olive oil, sesame oil, peanut oil, pearl barley oil, wheat germ oil, perilla oil, linseed oil, perilla oil, chia seed oil, sacha inchi oil, walnut oil, kiwi seed oil, salvia seed oil, grape seed oil, macadamia nut oil, hazelnut oil, pumpkin seed oil, camellia oil, tea seed oil, borage oil, palm oil, palm olein, palm stearin, coconut oil, palm kernel oil, cacao butter, monkey fat, shea butter, algae oil, etc.; animal fats and oils such as fish oil, seal oil, whale oil, lard, beef tallow, butter fat, etc.; or transesterified oils, hydrogenated oils, fractionated oils, etc. thereof. These may be used alone or in combination of two or more.

[0028] Fatty acids derived from fats and oils can be obtained by hydrolyzing fats and oils. Methods for hydrolyzing fats and oils include high-temperature, high-pressure hydrolysis and enzymatic hydrolysis. The high-temperature, high-pressure hydrolysis method is a method in which water is added to fats and oils and reacted under high temperature and high pressure conditions to obtain fatty acids and glycerin. The enzymatic hydrolysis method is a method in which water is added to fats and oils and reacted under low temperature conditions using an oil hydrolase as a catalyst to obtain fatty acids and glycerin. The hydrolysis reaction can be carried out according to a conventional method.

[0029] After the hydrolysis of the oil or fat, it is preferable to fractionate the hydrolysis reaction product to remove solids. Fractionation methods include solvent fractionation, natural fractionation (dry fractionation), and wetting agent fractionation. The precipitated solid may be removed by means of static separation, filtration, centrifugation, or a method in which the fatty acid is mixed with an aqueous solution of a wetting agent and then separated.

[0030] The esterification reaction between fatty acids derived from fats and oils and glycerin is preferably carried out under mild conditions by an enzymatic method, which is excellent in terms of flavor and the like. The amount of the enzyme used can be appropriately determined taking into consideration the activity of the enzyme. When an immobilized enzyme is used, the amount is preferably 1 to 30% by mass, more preferably 2 to 20% by mass, based on the total mass of the esterification reaction raw materials, from the viewpoint of improving the reaction rate. The reaction temperature of the esterification reaction is preferably 0 to 100° C., more preferably 20 to 80° C., and even more preferably 30 to 60° C., from the viewpoint of increasing the reaction rate and suppressing the deactivation of the enzyme. Moreover, the reaction time is preferably within 15 hours, more preferably 1 to 12 hours, and even more preferably 2 to 10 hours, from the viewpoint of industrial productivity of fats and oils. The fatty acid and glycerin can be brought into contact with each other by immersion, stirring, or passing the mixture through a column packed with immobilized lipase using a pump or the like.

[0031] The transesterification reaction (glycerolysis) between fats and oils and glycerin is preferably carried out by a chemical method from the viewpoint of reactivity. The amount of the catalyst used is preferably 0.001 to 3 mass %, more preferably 0.005 to 2 mass %, and even more preferably 0.01 to 1 mass %, based on the mass of the reaction raw materials, from the viewpoint of reactivity.

[0032] From the viewpoint of reactivity and suppressing the production of by-products, the reaction temperature is preferably 120 to 200° C., more preferably 150 to 180° C. From the viewpoint of reactivity, the reaction time is preferably within 6 hours, more preferably within 4 hours. In the present invention, the pressure in the reaction system during the ester exchange reaction is not particularly limited, and the reaction can be carried out under normal pressure or reduced pressure. In the case of normal pressure, it is preferable to carry out the reaction under a nitrogen gas flow from the viewpoint of reactivity.

[0033] After the esterification reaction or transesterification reaction (glycerolysis), a refining step that is usually used for fats and oils may be carried out, such as distillation, acid treatment, water washing, decolorization, and deodorization.

[0034] As shown in the examples below, when the fats and oils of the present invention were orally administered to obese / type 2 diabetes model mice (db / db mice), a significant increase in carbohydrate burning was observed in both the light and dark periods and overall (light and dark periods). That is, the fats and oils of the present invention have a carbohydrate burning promotion effect, and this effect is constant. Here, constant carbohydrate burning promotion means that the effect of carbohydrate burning promotion continues for a longer period, unlike temporary carbohydrate burning promotion that disappears within a few hours at most after a meal. If carbohydrate burning is promoted by the fats and oils of the present invention, energy consumption will increase, and it will be possible to prevent, improve, or treat the onset or progression of diseases or symptoms such as obesity, metabolic syndrome (visceral fat syndrome), and type 2 diabetes. Therefore, the fat or oil of the present invention can be a carbohydrate burning accelerator, can be used for the acceleration of carbohydrate burning, and can be used to produce a carbohydrate burning accelerator.

[0035] In the present invention, "promotion of carbohydrate burning" is a concept including increasing the degree of carbohydrate burning compared to when the oil or fat of the present invention is not administered or ingested. "Carbohydrate burning" refers to metabolizing carbohydrates ingested from food in each organ and using them as an energy source, and the amount of carbohydrate burning can be calculated, for example, by measuring breath using the formula shown in the Examples below. Note that carbohydrates are synonymous with carbohydrates and are classified into monosaccharides, disaccharides, oligosaccharides, and polysaccharides. "Use" may be for humans or non-human animals, and may be therapeutic or non-therapeutic. "Non-therapeutic" is a concept that does not include medical procedures, i.e., methods of surgery, therapy, or diagnosis of humans, and more specifically, does not include methods of surgery, therapy, or diagnosis performed by a physician, or a medical professional, or a person under the direction of a physician, on humans. "Prophylaxis" refers to preventing or delaying the onset of a disease or condition in an individual, or reducing an individual's risk of developing a disease or condition. "Amelioration" refers to improving a disease, symptom or condition, preventing or slowing the worsening of a disease, symptom or condition, or reversing, preventing or slowing the progression of a disease or symptom. Furthermore, "treatment" includes not only complete cure of a disease but also amelioration of symptoms.

[0036] The carbohydrate combustion promoter of the present invention can itself be a medicine, quasi-drug, cosmetic, food or feed for promoting carbohydrate combustion, or can be a material or preparation used by being incorporated into such a medicine, quasi-drug, cosmetic, food or feed.

[0037] The pharmaceutical (including quasi-drugs, the same applies below) contains the fat or oil of the present invention as an active ingredient for promoting carbohydrate burning. Furthermore, the pharmaceutical may contain a pharma- ceutically acceptable carrier, or other active ingredients, pharmacological ingredients, etc., as necessary, so long as the function of the active ingredient is not lost. The pharmaceutical agent may be administered in any dosage form. Examples of dosage forms include oral solid preparations such as tablets (including chewable tablets, etc.), capsules, granules, powders, and lozenges, oral liquid preparations such as oral liquids and syrups, and parenteral preparations such as injections, suppositories, inhalants, transdermal agents, and external preparations. The preferred dosage form is oral administration. The size of the dosage form can be adjusted as desired depending on the purpose of use. Preparations of such various dosage forms can be prepared according to standard methods by appropriately combining, as necessary, pharma- ceutically acceptable carriers such as excipients, binders, bulking agents, disintegrants, surfactants, lubricants, dispersants, buffers, osmotic pressure regulators, pH adjusters, emulsifiers, preservatives, stabilizers, thickeners, flow improvers, flavoring agents, foaming agents, fragrances, coating agents, diluents, and other medicinal ingredients.

[0038] The cosmetic contains the oil of the present invention as an active ingredient for promoting carbohydrate burning, and may further contain a carrier acceptable for cosmetics, or other active ingredients, medicinal ingredients, cosmetic ingredients, etc., as necessary, so long as the function of the active ingredient is not lost. Preferred examples of cosmetics containing the oil or fat of the present invention include face and body cosmetics (for example, lotions, gels, creams, packs, etc.), make-up cosmetics, face or body cleansers, and the like. Each of the preparations of such cosmetics can be manufactured according to a conventional method. Examples of carriers acceptable for cosmetics include various oils, surfactants, gelling agents, buffers, preservatives, antioxidants, solvents, dispersants, chelating agents, thickeners, UV absorbers, emulsion stabilizers, pH adjusters, colorants, and fragrances. Other active ingredients, medicinal ingredients, and cosmetic ingredients include, for example, plant extracts, bactericides, moisturizers, anti-inflammatory agents, antibacterial agents, keratolytic agents, cooling agents, antiseborrheic agents, cleansing agents, and makeup ingredients.

[0039] The food contains the oil or fat of the present invention as an active ingredient for promoting carbohydrate burning. Foods include foods that claim to promote carbohydrate burning and have been approved or notified to be labeled as such, as necessary (foods for specified health uses, foods with functional claims, foods for special dietary uses, etc.). Examples of such claims include "promotes carbohydrate burning," "increases the ability to metabolize carbohydrates," and "makes carbohydrates easier to consume." Foods that have been approved or notified to be labeled as such can be distinguished from general foods. The food may be in the form of a solid, semi-solid, or liquid (e.g., beverage). Examples include various food compositions (breads, cakes, noodles, confectioneries, frozen foods, ice creams, candies, toppings, soups, dairy products, shakes, beverages, seasonings, etc.), and nutritional supplement compositions in the same form as the oral preparations described above (solid preparations such as granules, powders, tablets, capsules, microcapsules, and lozenges). Foods of various forms can be prepared according to standard methods by appropriately combining the oil or fat of the present invention with any food material, or other active ingredients, or additives acceptable for foods (e.g., solvents, softeners, oils, emulsifiers, preservatives, acidulants, sweeteners, bittering agents, pH adjusters, stabilizers, colorants, UV absorbers, antioxidants, moisturizers, thickeners, adhesives, dispersants, flow improvers, humectants, aromatics, seasonings, flavor adjusters), etc.

[0040] The feed contains the oil or fat of the present invention as an active ingredient for promoting carbohydrate burning. The feed is preferably in the form of pellets, flakes, mash or liquid, and examples thereof include livestock feed for cows, pigs, chickens, sheep, horses, etc., small animal feed for rabbits, rats, mice, etc., and pet food for dogs, cats, small birds, etc. Feed can be prepared according to standard methods by appropriately combining the oil or fat of the present invention with other feed ingredients such as meat, protein, grains, brans, lees, sugars, vegetables, vitamins, minerals, gelling agents, shape-retaining agents, pH adjusters, seasonings, preservatives, nutritional supplements, etc.

[0041] The content of the oil or fat of the present invention in the formulation cannot be generally determined because it varies depending on the form of the formulation, but for example, it is preferably 0.5 mass% or more and preferably 99.8 mass% or less based on the total amount of the formulation.

[0042] The amount of the fat or oil of the present invention administered or used may be an amount that can achieve the effects of the present invention. The amount of the fat or oil administered or used may vary depending on the species, weight, sex, age, condition, or other factors of the subject, but in the case of oral administration (ingestion), the amount of the fat or oil of the present invention administered once per adult (60 kg) is, for example, 0.1 to 15 g. The carbohydrate burning promoter of the present invention may be administered or used according to any dosage regimen, and may be administered or used repeatedly or continuously, once or multiple times a day, for one day or more, preferably for seven days or more, more preferably for 14 days or more, and even more preferably for 28 days or more.

[0043] The carbohydrate burning accelerator of the present invention is preferably administered or used in the form of an oil or fat composition. When the carbohydrate combustion promoter is in the form of an oil composition, the content of the oil of the present invention in the oil composition is, from the viewpoint of usability, preferably 90% by mass or more, more preferably 95% by mass or more, and preferably 100% by mass or less, more preferably 99.9% by mass or less.

[0044] The oil and fat composition preferably contains an antioxidant from the viewpoints of flavor, oxidation stability, coloring inhibition, etc. The content of the antioxidant in the oil and fat composition is preferably 0.005 to 1.0 mass%, more preferably 0.04 to 0.75 mass%, and even more preferably 0.08 to 0.5 mass%, from the viewpoints of flavor, oxidation stability, coloring inhibition, etc. The antioxidant is not particularly limited, but is preferably at least one selected from natural antioxidants, lecithin, tocopherol, ascorbyl palmitate, ascorbyl stearate, dibutylhydroxytoluene (BHT), butylhydroxyanisole (BHA), and the like.

[0045] The subject to which the carbohydrate burning promoter of the present invention is administered or used is not particularly limited as long as it is a human or a non-human animal that requires or desires it. Preferred examples of subjects include mongoloids, etc. Non-human animals include non-human mammals such as apes and other primates. EXAMPLES

[0046] Preparation of test oils Oil and fat 1: 328 parts by mass of fatty acid obtained by enzymatic hydrolysis of DD Oil Type 2 (manufactured by Nippon Suisan Co., Ltd.) was mixed with 50 parts by mass of glycerin, and esterification reaction was carried out using 1,3-position selective lipase (manufactured by Novozymes Co., Ltd.) immobilized on ion exchange resin as a catalyst, and the immobilized enzyme was filtered off. Then, molecular distillation, acid treatment and water washing were carried out to obtain a treated oil. The treated oil was deodorized to obtain oil and fat 1. Oil and fat 2: 80 parts by mass of algae oil (manufactured by DSM) and 25 parts by mass of glycerin were mixed and subjected to a glycerolysis reaction using calcium hydroxide as a catalyst. Then, molecular distillation, acid treatment and water washing were performed to obtain a treated oil. The treated oil was deodorized to obtain oil and fat 2. Table 1 shows the glyceride compositions of oils and fats 1 and 2, the major fatty acid compositions in the oils and fats, and the major fatty acid compositions in diacylglycerol.

[0047] [Table 1]

[0048] The analytical methods for glyceride composition and fatty acid composition are as follows. (i) Glyceride composition of fats and oils The fats and oils were dissolved to 1 mg / ml using a chloroform / methanol (1 / 9) mixed solvent, diluted 10-fold with methanol, and then analyzed by high performance liquid chromatography (HPLC) together with a standard sample for calibration. <Standard sample> (1) For diacylglycerol: triolein / diolein / monoolein = 5 / 80 / 15 (by weight) (2) For triacylglycerol: triolein / diolein / monoolein = 90 / 5 / 5 (by weight) <HPLC analysis conditions> (Conditions) Apparatus: UHPLC (manufactured by Thermo Fisher Scientific) Column: L-column C8 (2.1×35 mm×5 μm) (manufactured by the National Institute of Technology and Evaluation) Column temperature: 40°C Sample injection volume: 10 μL Flow rate: 0.5 mL / min Mobile phase: (A) water / acetonitrile = 9 / 1 (B) isopropanol

[0049] [Table 2]

[0050] Detector: CAD (charged aerosol detector)

[0051] (ii) Fatty acid composition of fats and oils Fatty acid methyl esters were prepared according to the "Method for the Preparation of Fatty Acid Methyl Esters (2.4.1.-1996)" in the "Standard Analytical Test Methods for Oils and Fats" edited by the Japanese Oil Chemists' Society, and the obtained fat and oil samples were measured in accordance with American Oil Chemists. Society Official Method Ce 1f-96 (GLC method). <GLC analysis conditions> Apparatus: Agilent 7890B (Agilent Technologies) Column: CP-SIL88 50m x 0.25mm x 0.2μm (Agilent J&W) Carrier gas: 1.0mL He / min Injector: Split (1:50), T=300℃ Detector: FID, T=300℃ Oven temperature: 150℃ 5min hold → 1℃ / min temperature rise → 160℃ 5min hold → 2℃ / min temperature rise → 200℃ 10min hold → 10℃ / min temperature rise → 220℃ 5min hold

[0052] Test method overview Six-week-old obese / type 2 diabetes model mice (db / db mice, male) were acclimatized to the environment by pre-breeding for one week, and then divided into three groups of five mice each with the same body weight: high-fat / high-sucrose diet group (group 1), high-EPA-containing DAG group (group 2), and high-DHA-containing DAG group (group 3), and were bred on each of the diets listed in Table 3. The breeding environment was room temperature 23±2°C, humidity 55±10%, and lighting from 7:00 a.m. to 7:00 p.m.

[0053] [Table 3]

[0054] After four weeks of feeding, the mice were placed in the chamber of an exhaled gas analyzer (Arco 2000 system, Arco Systems) and allowed to acclimate for 24 hours. After that, the amount of carbohydrate burned was measured by measuring the exhaled breath for another 24 hours. The carbohydrate burning rate was calculated with reference to the Jequier formula (described in Parenter Enteral Nutr, Vol. 11, pp. 86-89, 1987). That is, the oxygen consumption (VO2) and carbon dioxide output (VCO2) measured using an exhaled gas analyzer were applied to the following formula.

[0055] Carbohydrate burning rate (mg / min) =1.689×VO2(mL / min)-1.689×VCO2(mL / min)-0.324×P(mg / min) VO2: Oxygen consumption rate VCO2: Carbon dioxide emission rate P: Protein burning rate (In this test, the protein burning rate was calculated assuming 12.5% ​​of the energy expenditure rate (EE). EE = 3.9 x VO2 (mL / min) + 1.1 x VCO2 (mL / min).)

[0056] Test results The results of the measurements are shown in Figures 1 to 3. Figure 1 shows the amount of carbohydrate burned per body weight during the light period (rest period), Figure 2 shows the dark period (active period), and Figure 3 shows the total amount (light and dark periods, 24 hours). Compared to the high-fat, high-sucrose diet group (Group 1), the high-DHA-containing DAG group (Group 3) did not show an increase in the amount of carbohydrate burned during the light and dark periods or overall, but the high-EPA-containing DAG group (Group 2) showed a significant increase in carbohydrate burned during the light and dark periods and overall. Significant differences were tested using an unpaired t-test (*P<0.05), and the analysis results were expressed as mean ± standard error.

Claims

1. A carbohydrate combustion promoter comprising, as an active ingredient, an oil and fat containing diacylglycerol in which 20% by mass or more of the constituent fatty acids is eicosapentaenoic acid.

2. The carbohydrate combustion promoter according to Claim 1, wherein the content of diacylglycerol in the oil and fat is 40% by mass or more.

3. The carbohydrate combustion promoter according to Claim 1, wherein the total content of saturated fatty acids in the fatty acids constituting diacylglycerol is 20% by mass or less.

4. The carbohydrate combustion promoter according to Claim 1, wherein the content of docosahexaenoic acid in the fatty acids constituting diacylglycerol is 30% by mass or less.

5. The carbohydrate combustion enhancer according to any one of Claims 1 to 4, which is used as a pharmaceutical or a food.

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