Container for sample and sample inspection method using said container

Abstract

The present invention relates to a container for samples. To improve optical or spectroscopic examination of a sample contained in the container, a container is proposed which comprises a base perpendicular to the container axis, a first wall adjacent to the base, and a second wall adjacent to the first wall, the second wall being inclined at an angle of 20° or more with respect to the container axis.

Description

[Technical field]

[0001] The present disclosure relates to a container for a sample, particularly a biological sample. Further, the present disclosure relates to a method of testing a sample and the use of the container to contain the sample during testing.

[0002] To examine a tissue sample, for example, tissue is removed from the body of a living organism (e.g., a human or an animal), the tissue is processed and then optically examined. The processing includes dehydration and clearing of the tissue, where the alcohol often used for dehydrating the tissue can be removed during the clearing process. The processed sample is cut into slices a few microns thick and then placed or mounted on a slide. This sample can also be stained. The slide with the sample is placed in a container and covered with a liquid. The sample can be glued directly to the bottom of the container. The container can be covered with (thin) glass. The sectioned and stained tissue sample can then be optically examined by microscopy, for example with transmitted light or fluorescence. The examination is performed outside the living organism. However, this examination does not provide any information about the three-dimensional position of the slices of the sample. That is, the three-dimensional position of the cells and tissue components within the tissue cannot be determined.

[0003] When a liquid is placed in a container, an interaction occurs between the liquid and the surface of the container wall. Depending on the material of the liquid and the container wall, a curvature of the liquid surface (concave or convex) is formed at the interface between the liquid and the container wall. The curved surface of the liquid, also called a concave meniscus, acts as a lens that can interfere with or distort the examination of a specimen inside the container covered by the liquid.

[0004] Accordingly, the present invention aims to provide a container that improves optical or spectroscopic inspection of a sample contained therein, particularly when the sample is covered with liquid and when the radiation source and radiation receiver are not in line with the sample.

[0005] It is another object of the present invention to provide a container that allows multiple sides of a specimen within the container to be inspected, thereby enabling three-dimensional inspection to be performed.

[0006] Yet another object is to provide a container that can be manufactured cost effectively.

[0007] This object is achieved by the subject matter and methods of the independent claims. Advantageous developments are set forth in the dependent claims.

[0008] The sample container includes a bottom, a first wall, and a second wall. The container axis is oriented or disposed perpendicular to the bottom. The first wall is adjacent to the bottom. The second wall is adjacent to the first wall. The second wall is inclined at an angle of 20° or more with respect to the container axis. That is, the container has a flat bottom surface, and the first wall surrounding the bottom surface and projecting upward is adjacent to the bottom surface. Thus, the first wall forms a first receiving space together with the bottom surface. The first wall is adjacent to a second wall having a different inclination than the first wall. Thus, the second wall surrounds a second receiving space above the first receiving space.

[0009] The sample container may include a bottom, a first wall, and a second wall. The container axis is oriented or disposed perpendicular to the bottom. The first wall is adjacent to the bottom and the second wall is adjacent to the first wall. The cross-sectional area of ​​the container at half the height of the first wall may be smaller (by 5% or more, or by 10% or more) than the cross-sectional area of ​​the container at half the height of the second wall. The half height may be half the total height of each wall in the container axis direction.

[0010] That is, the container has a flat bottom surface, and a first wall portion that surrounds the bottom surface and protrudes upward is adjacent to the bottom surface. As a result, the first wall portion forms a first receiving space together with the bottom surface. A second wall portion that has a different slope from the first wall portion is adjacent to the first wall portion. As a result, the second wall portion surrounds a second receiving space above the first receiving space.

[0011] The configuration of the second wall allows liquid to be introduced into the container such that any tilt in the liquid level that would interfere with optical or spectroscopic examination is outside the observation area. As described above, the sample may be attached to a slide and the slide may be placed in the container.

[0012] In a plane parallel to the bottom or perpendicular to the container axis and bounded by the first wall (at least on one side), the liquid level is substantially (±5°) flat, substantially (±5°) not curved, substantially (±5°) parallel to the bottom or substantially (±5°) perpendicular to the container axis. This allows the sample to be placed on the entire bottom without interfering with the inspection. The sample is thus placed in the first receiving space and surrounded by liquid. The liquid is filled up to the second receiving space. Thus, a meniscus can only be formed in the edge region of the second receiving space, which does not have a destructive effect when the sample is irradiated with radiation for optical inspection.

[0013] The sample may be a biological sample, such as a tissue sample. The sample may be a mixture of substances, such as a homogeneous or heterogeneous mixture of substances. The sample may be a pure substance.

[0014] The bottom is 200mm 2 In particular, the bottom may have an area of ​​400 mm 2 or more than 500mm 2 The area of ​​the bottom part may be 5000 mm 2 Less than 4000mm, preferably 4000mm 2 Less than or equal to 3000 mm, more preferably 2 Less than or equal to 2500 mm, preferably less than 2500 mm 2 The area of ​​the base is particularly preferably 200 mm 2 ~5000mm 2 It is.

[0015] At least the surface of the bottom facing the interior of the container may be configured to be flat over its entire extent.

[0016] The first wall may be integrally formed with the base.

[0017] An angle may be formed between the first wall and the container axis. The first wall may be inclined at this angle with respect to the container axis. The angle between the container axis and the first wall may be 0.5° or more. The angle between the container axis and the first wall is preferably 1.0° or more or 1.5° or more. The angle between the container axis and the first wall may be 50° or less, preferably 40° or less, more preferably 30° or less, more preferably 25° or less, more preferably 10° or less, more preferably 5° or less. In particular, the angle between the container axis and the first wall is 0.5° to 50°, preferably 0.5° to 30°, more preferably 0.5° to 10°. The angle between the container axis and the first wall is particularly preferably 1.0° to 3.0° or 1.5° to 2.5°.

[0018] There may be no angle formed between the first wall and the container axis. The first wall may be parallel to the container axis. The angle between the first wall and the bottom may be substantially 90°.

[0019] The first wall may have a height (in the container axial direction) of 5 mm or more, preferably 7 mm or more, more preferably 10 mm or more, more preferably 15 mm or more, more preferably 24 mm or more. The height of the first wall may be 100 mm or less, preferably 80 mm or less, more preferably 60 mm or less, more preferably 50 mm or less, more preferably 30 mm or less, more preferably 25 mm or 15 mm or less. The height of the first wall may be 5 mm to 100 mm, preferably 7 mm to 60 mm.

[0020] The first wall may have a width (perpendicular to the container axial direction) of 5 mm or more, more preferably 10 mm or more, preferably 20 mm or more, more preferably 30 mm or more, more preferably 35 mm or 40 mm or more. The width of the first wall may be 150 mm or less, preferably 130 mm or less, more preferably 110 mm or less, more preferably 90 mm or less, more preferably 70 mm or less, more preferably 50 mm or less. The width of the first wall may be 5 mm to 150 mm, preferably 10 mm to 100 mm, more preferably 30 mm to 60 mm. This width may be the maximum width of the first wall.

[0021] The width of the first wall may be at least 5mm greater than its height.

[0022] The first wall may be configured to be planar.

[0023] The second wall may be integrally formed with the first wall.

[0024] An angle is formed between the second wall and the container axis. The second wall is inclined at this angle to the container axis. The angle may be 30° or more, preferably 40° or more, more preferably 50° or more, more preferably 55° or more, more preferably 60° or more. The angle between the second wall and the container axis may be 90° or less. The angle between the second wall and the container axis is preferably 80° or less, more preferably 75° or less. The angle between the second wall and the container axis may be 20° to 90°, preferably 30° to 80°, more preferably 40° to 70°.

[0025] The second wall may have a height (in the container axial direction) of 0.5 mm or more, preferably 1 mm or more, more preferably 1.5 mm or more. The height of the second wall may be 30 mm or less, preferably 20 mm or less, more preferably 15 mm or less, more preferably 10 mm or less, more preferably 5 mm or less. The height of the second wall may be 0.5 mm to 30 mm, preferably 1 mm to 20 mm.

[0026] The second wall may have a width (perpendicular to the container axial direction) of 5 mm or more, more preferably 10 mm or more, preferably 20 mm or more, more preferably 30 mm or more, more preferably 40 mm or 50 mm or more. The width of the second wall may be 150 mm or less, preferably 130 mm or less, more preferably 110 mm or less, more preferably 90 mm or less, more preferably 70 mm or less, more preferably 60 mm or less. The width of the second wall may be 5 mm to 150 mm, preferably 10 mm to 100 mm, more preferably 40 mm to 70 mm. This width may be the maximum width of the first wall.

[0027] The third wall may be adjacent to the second wall. The third wall may be integrally formed with the second wall, such that the third wall surrounds a third receiving space disposed above the second receiving space.

[0028] An angle may be formed between the third wall and the container axis. The third wall may be inclined at this angle with respect to the container axis. The angle may be 1° or more, preferably 2° or more, more preferably 3° or more, more preferably 4° or more, more preferably 5° or more. The angle between the third wall and the container axis may be 50° or less. The angle between the third wall and the container axis is preferably 40° or less, more preferably 30° or less, more preferably 20° or less, more preferably 10° or less, more preferably 8° or less. The angle between the third wall and the container axis may be 1° to 50°, preferably 2° to 30°, more preferably 3° to 10°.

[0029] The third wall may have a height (in the container axial direction) of 1 mm or more, preferably 5 mm or more, more preferably 8 mm or more. The height of the third wall may be 100 mm or less, preferably 50 mm or less, more preferably 30 mm or less, more preferably 20 mm or less, more preferably 15 mm or less. The height of the third wall may be 1 mm to 100 mm, preferably 1 mm to 50 mm, more preferably 5 mm to 20 mm.

[0030] The third wall may have a width (perpendicular to the container axial direction) of 5 mm or more, more preferably 10 mm or more, more preferably 20 mm or more, more preferably 30 mm or more, more preferably 40 mm or 50 mm or more. The width of the third wall may be 150 mm or less, preferably 130 mm or less, more preferably 110 mm or less, more preferably 90 mm or less, more preferably 80 mm or less, more preferably 70 mm or less. The width of the third wall may be 5 mm to 150 mm, preferably 10 mm to 100 mm, more preferably 40 mm to 70 mm. This width may be the maximum width of the first wall.

[0031] The container may have an opening on the side opposite the bottom. The opening may be defined by one or more third walls. The opening may have a larger area than the bottom. The larger area of ​​the container opening (relative to the bottom) allows imaging at the bottom of the container to make the best possible use of the numerical aperture of the objective lens above the sample in the container. This container configuration reduces the obstruction of the path of the light emitted by the sample. If necessary, the lens may be lowered into the container, in particular into the third receiving space formed by the third wall.

[0032] The (angle or inclination of) the wall of the wall or walls can be selected in order to utilise the numerical aperture of the objective, in particular to utilise it as optimally as possible.

[0033] The edge of the container opening may have a constant height (in the container axial direction). The plane defined by the container opening may be parallel to the base.

[0034] The second wall may be adjacent to the first wall in the vessel axial direction. The third wall may be adjacent to the second wall in the vessel axial direction.

[0035] The first wall may have a different inclination relative to the container axis than the second wall.The second wall may have a different inclination relative to the container axis than the third wall.

[0036] The first wall, the second wall, and / or the third wall may be a wall of a first wall surface of the container. The first wall surface may be composed of or include the first wall, the second wall, and the third wall.

[0037] The container, particularly the base, may have a polygonal basic shape having a first wall, a second wall, a third wall and a fourth wall.

[0038] The second wall surface may include or consist of a first wall portion, a second wall portion, and a third wall portion. The first wall portion, the second wall portion, and the third wall portion of the second wall surface may be equal to the first wall portion, the second wall portion, and the third wall portion of the first wall surface, respectively.

[0039] The third wall surface may include or consist of a first wall portion, a second wall portion, and a third wall portion. The first wall portion, the second wall portion, and the third wall portion of the third wall surface may be equal to the first wall portion, the second wall portion, and the third wall portion of the first wall surface, respectively.

[0040] The fourth wall surface may include or consist of a first wall portion, a second wall portion, and a third wall portion. The first wall portion, the second wall portion, and the third wall portion of the fourth wall surface may be equal to the first wall portion, the second wall portion, and the third wall portion of the first wall surface, respectively.

[0041] The polygonal base shape may be a rectangular base shape, in particular a square base shape. The polygonal base shape is preferably a rectangle rather than a square. Similarly, the polygonal base shape may be an n-sided shape (n being an integer greater than 4).

[0042] The basic shape of the container, in particular the bottom, may correspond to an ellipse, in particular a circle, with a chord, the chord corresponding to the first edge of the wall, in which case the point of incidence of the light lies in the area of ​​the chord, i.e. in the planar area.

[0043] Similarly, the first wall portion, the second wall portion, and the third wall portion of the third wall surface may have a different inclination (relative to the container axis) than the first wall portion, the second wall portion, and the third wall portion of the first wall surface, respectively.

[0044] The first wall portion of the third wall surface may have a greater inclination relative to the container axis than the first wall portion of the first wall surface. The first wall portion of the third wall surface may be inclined relative to the container axis at an angle that is preferably 2° or more, or 5° or more, greater than the first wall portion of the first wall surface.

[0045] The first wall portion of the third wall surface may be inclined at an angle of no more than 30°, preferably no more than 20°, more preferably no more than 15° greater relative to the container axis than the first wall portion of the first wall surface.

[0046] The second wall portion of the third wall surface may have a smaller inclination relative to the container axis than the second wall portion of the first wall surface. The second wall portion of the third wall surface may have a smaller inclination relative to the container axis than the second wall portion of the first wall surface, preferably by 2° or more or 5° or more.

[0047] The second wall portion of the third wall may be inclined at an angle of no more than 30°, preferably no more than 20°, more preferably no more than 15° less relative to the container axis than the second wall portion of the first wall.

[0048] The first wall portion, the second wall portion, and the third wall portion of the second wall surface may have a different inclination (relative to the container axis) than the first wall portion, the second wall portion, and the third wall portion of the first wall surface, respectively.

[0049] The first wall portion of the second wall surface may have a greater inclination relative to the container axis than the first wall portion of the first wall surface, the inclination of the first wall portion of the second wall surface relative to the container axis being preferably at least 1° or at least 2° greater than the inclination of the first wall portion of the first wall surface relative to the container axis.

[0050] The first wall portion of the second wall surface may be inclined at an angle of no more than 20°, preferably no more than 10°, more preferably no more than 5° greater relative to the container axis than the first wall portion of the first wall surface.

[0051] Similarly, the second wall portion of the second wall surface may have a smaller inclination relative to the container axis than the second wall portion of the first wall surface, preferably by more than 1° or more than 2° less than the inclination of the second wall portion of the first wall surface relative to the container axis.

[0052] The second wall portion of the second wall surface may be inclined at an angle of no more than 20°, preferably no more than 10°, more preferably no more than 5° less relative to the container axis than the first wall portion of the first wall surface.

[0053] The first wall portion, the second wall portion, and the third wall portion of the fourth wall surface may have a different inclination (relative to the container axis) than the first wall portion, the second wall portion, and the third wall portion of the first wall surface, respectively.

[0054] The first wall portion of the fourth wall surface may have a greater inclination relative to the container axis than the first wall portion of the first wall surface, the inclination of the first wall portion of the fourth wall surface relative to the container axis being preferably at least 1° or at least 2° greater than the inclination of the first wall portion of the first wall surface relative to the container axis.

[0055] The first wall portion of the fourth wall surface may be inclined at an angle of no more than 20°, preferably no more than 10°, more preferably no more than 5° greater relative to the container axis than the first wall portion of the first wall surface.

[0056] The second wall portion of the fourth wall surface may have a smaller inclination relative to the container axis than the second wall portion of the first wall surface, preferably at least 1° or at least 2° smaller than the inclination of the second wall portion of the first wall surface relative to the container axis.

[0057] The second wall portion of the fourth wall surface may be inclined at an angle of no more than 20°, preferably no more than 10°, more preferably no more than 5° less relative to the container axis than the first wall portion of the first wall surface.

[0058] The sample container may include a bottom and a first wall. The container axis is oriented or disposed perpendicular to the bottom. The first wall is adjacent to the bottom. The first wall is inclined at an angle of 1.9°±0.7° or n multiples thereof, where n is an integer greater than 1, with respect to the container axis.

[0059] An inclination of the vessel axis relative to the first wall of 1.9°±0.7° or a multiple n thereof (n being an integer greater than 1) may be applied to all vessels described herein.

[0060] A slope of 1.9°±0.7° for the first wall defines a range of 1.2° to 2.6°. A multiple of n (n>1 is an integer) corresponds to a value within this range multiplied by n. For example, 1.5° is in the range of 1.9°±0.7°, and if n is 3, a slope of 4.5° is given. Alternatively, 2.2° is in the range of 1.9°±0.7°, and if n is 3, a slope of 6.6° is given.

[0061] The first wall is preferably inclined at an angle of 1.9°±0.5° or a multiple of n thereof, where n is an integer greater than 1, relative to the container axis. More preferably, the first wall is inclined at an angle of 1.9°±0.3° or a multiple of n thereof, where n is an integer greater than 1, relative to the container axis. More preferably, the first wall is inclined at an angle of 1.9°±0.15° or a multiple of n thereof, where n is an integer greater than 1. The integer value of n may be 10 or less, preferably 7 or less, more preferably 5 or less, more preferably 3 or less.

[0062] The container may be an injection molded container.The container may be manufactured by injection molding.

[0063] The entire container may be integrally molded.

[0064] Preferably, the material of the container is transparent. The container may be completely transparent. All walls of the container may be transparent. At least one wall of the container may be transparent, preferably at least two walls of the container are transparent. At least a first wall of the container may be transparent. In particular, the first wall of at least the first wall is transparent. Alternatively or additionally, the first wall of the second wall may be transparent. Alternatively or additionally, the first wall of the third wall may be transparent. Alternatively or additionally, the first wall of the fourth wall may be transparent. The container may be transparent at least where light is irradiated.

[0065] The container may comprise or consist of a thermoplastic resin. The container may comprise or consist of one or more materials. The first wall of the first wall may comprise or consist of a different material than the second wall and / or the third wall of the first wall. Alternatively or additionally, the first wall of the second wall may comprise or consist of a different material than the second wall and / or the third wall of the second wall. Alternatively or additionally, the first wall of the third wall may comprise or consist of a different material than the second wall and / or the third wall of the third wall. Alternatively or additionally, the first wall of the fourth wall may comprise or consist of a different material than the second wall and / or the third wall of the fourth wall.

[0066] The container may comprise one or more pigments. In particular, the container comprises a pigment suitable for laser marking. A pigment suitable for laser marking is capable of changing colour when irradiated with a laser.

[0067] The container may be constructed of a material suitable for manufacture by injection molding.

[0068] The opening of the container may be defined by one or more third walls.

[0069] The wall thickness of the container may be 0.5 mm to 5.0 mm, preferably 1.0 mm to 4.0 mm, and more preferably 1.0 mm to 2.0 mm.

[0070] The height of the container may be less than 150 mm, preferably less than 120 mm, more preferably less than 100 mm, more preferably less than 80 mm, more preferably less than 50 mm, more preferably less than 30 mm, more preferably less than 25 mm, more preferably less than 15 mm. In particular, the height of the container is 5 mm or more, or 10 mm or more.

[0071] The container may hold a volume of less than 100 ml, preferably less than 75 ml, more preferably less than 50 ml, even more preferably less than 30 ml. Preferably, the container holds a volume of 5 ml or more, especially 10 ml or more. In particular, the container may hold a volume of 5 ml to 50 ml.

[0072] The third wall surface may be opposite to the first wall surface, and the fourth wall surface may be opposite to the second wall surface.

[0073] The container may comprise or consist of a (thermoplastic) polymer. The polymer may be a copolymer, in particular a block copolymer.

[0074] The polymer may comprise units resulting from (complete) hydrogenation of polystyrene units. The polymer may comprise polycyclohexylethene units.

[0075] The polymer may comprise units resulting from (complete) hydrogenation of polybutadiene units. The polymer may comprise ethylene-1-butene copolymer units.

[0076] This polymer can be obtained or prepared by (complete) hydrogenation of a styrene-butadiene copolymer.

[0077] The polymer may be a block copolymer, especially a cyclic block copolymer.

[0078] The polymer is particularly transparent in the wavelength range of 380 nm to 780 nm.

[0079] The container may be partially made of a particular material, and preferably the entire container is made of that material.

[0080] The material is 1.00g / cm 3 Less than 0.98 g / cm 3 Less than or equal to 0.96 g / cm 3 Below 0.92 g / cm, particularly preferably 3 ~0.96g / cm 3 The density can be measured according to ASTM D792.

[0081] The material may have a water absorption of less than 5%, preferably less than 2.0%, more preferably less than 0.5%. Water absorption may be measured according to ASTM D570.

[0082] The material is 1.0 cm 3 / 10 minutes or more, preferably 7.0 cm 3 / 10 minutes or more, preferably 100.0 cm 3 / 10 minutes or more, preferably 2.0 cm 3 / 10 minutes~250.0cm 3 In particular, the melt flow rate of the material may be 5.0 cm / 10 min. 3 / 10 minutes~20.0cm 3 The melt flow rate can be measured at a temperature of 260° C. with a weight of 2.6 kg according to ASTM D1238.

[0083] The material may have a transmittance of 50% or more, preferably 70% or more, more preferably 85% or more, more preferably 90% or more. The 3 mm transmittance may be measured according to ASTM D1003.

[0084] The material may have a transmittance at wavelengths of 488 nm and 638 nm of 50% or more, preferably 60% or more, more preferably 70% or more, and more preferably 80% to 85%.

[0085] The material may have a haze of less than 5.0%, preferably less than 3.0%, more preferably less than 1.5%, more preferably less than 1.0%. Haze at 3 mm may be measured according to ASTM D1003.

[0086] The material may have a refractive index less than 5.0, preferably less than 3.0, more preferably less than 2.5, more preferably less than 2.0, more preferably less than 1.6, more preferably between 1.46 and 1.56.

[0087] The material may have a Vicat softening point of 70° C. or higher, preferably 85° C. or higher, more preferably 100° C. or higher, more preferably 90° C. to 130° C. In particular, the Vicat softening point is 118° C. to 128° C. The Vicat softening point may be measured according to ASTM D1525 at 1 kg, 50° C. / hour.

[0088] The material may have a heat deflection temperature of 50° C. or more, preferably 60° C. or more, more preferably 75° C. or more, more preferably between 60° C. and 115° C. In particular, the heat deflection temperature is between 97° C. and 108° C. The heat deflection temperature may be measured according to ASTM D648 at 0.455 MPa and 2° C. / min.

[0089] The material may have a flexural strength of 30 MPa or more, preferably 40 MPa or more, more preferably 50 MPa or more, more preferably 40 MPa to 80 MPa. In particular, the flexural strength is 65 MPa to 75 MPa. The flexural strength can be measured according to ASTM D790.

[0090] The material may have a flexural modulus of 600 MPa or more, preferably 1000 MPa or more, more preferably 1400 MPa or more, more preferably 1000 MPa to 3000 MPa. In particular, the flexural modulus is 2200 MPa to 2600 MPa. The flexural modulus can be measured according to ASTM D790.

[0091] The material may have a tensile strength (YP or BP) of 15 MPa or more, preferably 25 MPa or more, more preferably 30 MPa or more, more preferably 20 MPa to 50 MPa. In particular, the tensile strength (YP or BP) is 35 MPa to 48 MPa. The tensile strength (YP or BP) can be measured according to ASTM D638.

[0092] The material may have a strain of 5% or more, preferably 8% or more, more preferably 10% or more, more preferably 8% to 40%. In particular, the material has a strain of 20% to 29%. The strain may be measured according to ASTM D638.

[0093] The material may have an Izod impact strength of 1 kgcm / cm or more, preferably 1.5 kgcm / cm or more, more preferably 2.0 kgcm / cm or more, more preferably 1.5 kgcm / cm to 5.0 kgcm / cm. In particular, the Izod impact strength is 2.8 kgcm / cm to 4.4 kgcm / cm. The Izod impact strength can be measured according to ASTM D256.

[0094] The material may be chemically resistant to methyl salicylate (2-hydroxybenzoic acid methyl ester), dibenzyl ether (1,1'-[oxybis(methylene)]bisbenzene), a mixture of 1 part benzyl alcohol (phenylmethanol) and 2 parts benzyl benzoate (benzoic acid phenylmethyl ester), a mixture of 4 parts benzyl benzoate (phenoxybenzene, 1,1'-oxybisbenzene) and 1 part diphenyl ether, ethyl cinnamate (ethyl 3-phenylprop-2-enoate), and / or 2,2'-thiodiethanol (thiodiglycol, bis(2-hydroxyethyl)sulfide). Chemical resistance may be determined by immersing a test specimen for testing the tensile strength of the material or of a granule of the material (e.g., according to ASTM D638) in a liquid (solvent) at room temperature (23°C) for 2 days (48 hours or 7 days) (covered by the liquid). After two days, the weight loss is measured, and if the weight loss is less than 5.0%, preferably less than 1.0%, the material is chemically resistant to the liquid. Also, after two days, the breaking elongation is measured (e.g., according to ASTM D638), and if the breaking elongation is reduced by less than 50%, preferably less than 10%, compared to a test piece that is not immersed in the liquid, the material is chemically resistant to the liquid.

[0095] The material may have an autofluorescence of 50% or less, preferably 40% or less, more preferably 30% or less, more preferably 25% or less, relative to the autofluorescence of polystyrene. The autofluorescence of the material and polystyrene may be measured at an excitation wavelength of 350 nm (sample thickness 1 mm), and the ratio of the measured autofluorescence values ​​may be calculated (autofluorescent material / autofluorescent polystyrene). The autofluorescence may be considered in the wavelength range of 400 nm to 460 nm. In particular, the autofluorescence at a wavelength of 420 nm may be considered, in which case the autofluorescence of the material relative to the autofluorescence of polystyrene may be 50% or less, preferably 40% or less, more preferably 30% or less, more preferably 20% or less, more preferably 15% or less.

[0096] The material does not have to exhibit any (measurable) autofluorescence when excited in the wavelength range of 400 nm to 900 nm.

[0097] Each of the containers disclosed herein can be used in a method for testing a sample. The sample may be a biological sample. The sample is placed in the container. The sample is irradiated and light emitted from the sample is recorded.

[0098] The sample may be irradiated with a radiation source, in particular a laser.

[0099] The emission of the sample may be fluorescence.

[0100] The sample may be illuminated through a first wall of the first wall of the container.

[0101] The emission of the sample can be recorded substantially perpendicular (±10°) to the direction of illumination.

[0102] The sample's emitted light can be collected through an objective lens.

[0103] Each of the containers disclosed herein can be accommodated for containing a sample during testing of the sample.

[0104] The examination may be microscopy, more preferably light sheet microscopy, even more preferably light sheet fluorescence microscopy.

[0105] Exemplary embodiments of the disclosure are illustrated in the drawings and are described in more detail below. [Brief description of the drawings]

[0106] [Figure 1] FIG. 1 is an isometric view of a container 100. [Diagram 2] FIG. 1 is a view of the container 100 from below. [Diagram 3] 3 shows cross section AA of the container 100 shown in FIG. [Figure 4] 3 shows a cross section BB of the container 100 shown in FIG. [Diagram 5] FIG. 2 is an isometric view of a container 200. [Figure 6]FIG. 2 is a view of the container 200 from below. [Figure 7] 7 shows a cross section CC of the container 200 shown in FIG. [Figure 8] 7 shows a cross section DD of the container 200 shown in FIG. [Figure 9] A system 400 for measuring a sample is shown.

[0107] Figures 1 and 2 show a vessel 100. In Figure 1, the vessel 100 is shown in an isometric view, and in Figure 2, the vessel 100 is shown in a view from below. In Figure 2, two cross sections AA and BB are outlined. Cross sections AA and BB are shown in Figures 3 and 4.

[0108] The container 100 includes a bottom 110 and a first wall 101. The container 100 may include a second wall 102, a third wall 103, and a fourth wall 104.

[0109] In general, the container 100 has a polygonal basic shape. In particular, the bottom 110 of the container 100 is polygonal. The polygonal basic shape or polygonal shape is in particular rectangular.

[0110] The first wall surface 101 and the third wall surface 103 may have a width larger than the second wall surface 102 and the fourth wall surface 104. The width of the first wall surface 101 and the third wall surface 103 is preferably 1.5 times or more larger than the width of the second wall surface 102 and the fourth wall surface 104.

[0111] At cross section AA in FIG. 3, the first wall 101, the base 110 and the third wall 103 are shown in cross section.

[0112] The inner surface of bottom 110 (facing the interior of the container) may be flat. Bottom 110 may be provided with an injection dome 118 such that the presence of injection dome 118 results in the inner surface of bottom 110 being flat. Injection dome 118 may be provided during injection molding of container 100.

[0113] The bottom 110, for example an inner surface of the bottom 110, may define a container axis 105. The container axis may extend perpendicular to the bottom 110. The bottom 110 may define a plane perpendicular to the container axis 105.

[0114] The first wall surface 101 may include a first wall portion 121 , a second wall portion 131 , and a third wall portion 141 .

[0115] The first wall 121 may be (directly) adjacent to the bottom 110, in particular in the direction of the container axis 105. The second wall 131 may be (directly) adjacent to the first wall, in particular in the direction of the container axis 105. The third wall 141 may be (directly) adjacent to the second wall 131, in particular in the direction of the container axis 105.

[0116] An angle α121 may be formed between the container axis 105 and the first wall 121. The first wall 121 may be inclined at this angle α121 relative to the container axis 105. The first wall 121 is preferably inclined outwardly (away from the interior of the container 100) relative to the container axis 105.

[0117] The angle α121 may be 1.9°±0.7° or n times thereof (n is an integer greater than 1). Preferably, the angle α121 is 1.9°±0.5° or n times thereof (n is an integer greater than 1), more preferably 1.9°±0.3° or n times thereof (n is an integer greater than 1), and more preferably 1.90°±0.15° or n times thereof (n is an integer greater than 1).

[0118] An angle α131 may be formed between the container axis 105 and the second wall 131. The second wall 131 may be inclined (outwardly) at the angle α131 relative to the container axis 105.

[0119] An angle α141 may be formed between the container axis 105 and the third wall 141. The third wall 141 may be inclined (outwardly) at the angle α141 relative to the container axis 105.

[0120] The first wall 121 , the second wall 131 and the third wall 141 may each be inclined (outwardly) with respect to the container axis 105 .

[0121] The cross section of the container 100 at the bottom 110 (cross section parallel to the bottom 110) may be smaller than the cross section of the container 100 at the first wall 121 (cross section at the height of the central part of the first wall 121 in the direction of the container axis 105). The cross section of the container 100 at the first wall 121 (cross section at the height of the central part of the first wall 121 in the direction of the container axis 105) may be smaller than the cross section of the container 100 at the second wall 131 (cross section at the height of the central part of the second wall 131 in the direction of the container axis 105). The cross section of the container 100 at the second wall 131 (cross section at the height of the central part of the second wall 131 in the direction of the container axis 105) may be smaller than the cross section of the container 100 at the third wall 141 (cross section at the height of the central part of the third wall 141 in the direction of the container axis 105).

[0122] The cross section of the container 100 may increase (continuously, preferably with a non-constant gradient) in the direction of the container axis 105. The design of the container 100 allows for less liquid (clarifying agent) to be introduced for sample testing, thus reducing the amount of liquid or clarifying agent.

[0123] A user can grip the container 100 at the top region (the third wall(s)). This can reduce or avoid fingerprints or other surface contamination on the surface of the first wall(s). The first wall(s) can be transilluminated by radiation, as described with reference to FIG. 9.

[0124] Similarly, a label may be provided in the upper region (one or more third walls) of the container 100. The label may include a data matrix code and / or a serial number. The label may include information about the container 100 and / or about the sample contained or contained therein and / or about the liquid that is or has been introduced into the container 100.

[0125] Between the first wall 121 and the second wall 131, there may be a kink in the inclination of the walls 121, 131 relative to the container axis 105. The kink may result from an abrupt or discontinuous change in the inclination relative to the container axis 105. Also, between the second wall 131 and the third wall 141, there may be a kink in the inclination of the walls 131, 141 relative to the container axis.

[0126] The kink between the first wall 121 and the second wall 131 and / or the kink between the second wall 131 and the third wall 141 may serve as a marker of the intended filling level or amount of, for example, a clarifying agent in the container 100.

[0127] The angle α121 between the container axis 105 and the first wall 121 may be smaller than the angle α131 between the container axis 105 and the second wall 131. The angle α131 between the container axis 105 and the second wall 131 may be larger than the angle α141 between the container axis 105 and the third wall 141. The angle α121 between the container axis 105 and the first wall 121 may be smaller than the angle α141 between the container axis 105 and the third wall 141.

[0128] The third wall 103 may include a first wall portion 123 , a second wall portion 133 , and a fourth wall portion 143 .

[0129] The first wall portion 123 of the third wall surface 103 may be (directly) adjacent to the bottom portion 110. The second wall portion 133 of the third wall surface 103 may be (directly) adjacent to the first wall portion 133 of the third wall surface 103. The third wall portion 143 of the third wall surface 103 may be (directly) adjacent to the second wall portion 133 of the third wall surface 103.

[0130] An angle α123 may be formed between the container axis 105 and the first wall portion 123 of the third wall surface 103. The first wall portion 123 may be inclined at this angle α123 relative to the container axis 105. The first wall portion 123 of the third wall surface 103 is preferably inclined outwardly (away from the interior of the container 100) relative to the container axis 105.

[0131] An angle α133 may be formed between the container axis 105 and the second wall portion 133 of the third wall surface 103. The second wall portion 133 of the third wall surface 103 may be inclined (outwardly) at the angle α133 relative to the container axis 105.

[0132] An angle α143 may be formed between the container axis 105 and the third wall portion 143. The third wall portion 143 of the third wall surface 103 may be inclined (outwardly) at the angle α143 relative to the container axis 105.

[0133] The first wall portion 123 of the third wall surface 103, the second wall portion 131 of the third wall surface 103, and the third wall portion 141 of the third wall surface 103 may each be inclined (outwardly) with respect to the container axis 105.

[0134] Between the first wall portion 123 of the third wall surface 103 and the second wall portion 133 of the third wall surface 103, there may be a kink in the inclination of the walls 123, 133 with respect to the container axis 105. Also, between the second wall portion 133 of the third wall surface 103 and the third wall portion 143 of the third wall surface 103, there may be a kink in the inclination of the walls 133, 143 with respect to the container axis 105.

[0135] The angle α123 between the container axis 105 and the first wall 123 of the third wall 103 may be smaller than the angle α133 between the container axis 105 and the second wall 133 of the third wall 103. The angle α133 between the container axis 105 and the second wall 133 of the third wall 103 may be larger than the angle α143 between the container axis 105 and the third wall 143 of the third wall 103. The angle α123 between the container axis 105 and the first wall 123 may be larger than the angle α143 between the container axis 105 and the third wall 143 of the third wall 103.

[0136] The first wall portion 121 of the first wall surface 101 may face the first wall portion 123 of the third wall surface 103. The first wall portion 121 of the first wall surface 101 may have substantially the same height (±10%) as the first wall portion 123 of the third wall surface 103.

[0137] The second wall portion 131 of the first wall surface 101 may face the second wall portion 133 of the third wall surface 103. The second wall portion 131 of the first wall surface 101 may have substantially the same height as the second wall portion 133 of the third wall surface 103 (±10%).

[0138] The third wall portion 141 of the first wall surface 101 may face the third wall portion 143 of the third wall surface 103. The third wall portion 141 of the first wall surface 101 may have substantially the same height as the third wall portion 143 of the third wall surface 103 (±10%).

[0139] The angle α121 between the container axis 105 and the first wall portion 121 of the first wall surface 103 may be smaller than the angle α123 between the container axis 105 and the first wall portion 123 of the third wall surface 103.

[0140] The angle α131 between the container axis 105 and the second wall portion 131 of the first wall 101 may be greater than the angle α123 between the container axis 105 and the second wall portion 133 of the third wall 103 .

[0141] Figure 4 shows cross section BB shown in Figure 2. The bottom 110, the second wall 102 and the fourth wall 104 are cross-sectioned.

[0142] The second wall 102 may include a first wall portion 122 , a second wall portion 132 , and a third wall portion 142 .

[0143] The first wall 122 of the second wall 102 may be (directly) adjacent to the bottom 110, in particular in the direction of the container axis 105. The second wall 132 of the second wall 102 may be (directly) adjacent to the first wall 122 of the second wall 102, in particular in the direction of the container axis 105. The third wall 142 of the second wall 102 may be (directly) adjacent to the second wall 132 of the second wall 102, in particular in the direction of the container axis 105.

[0144] An angle α122 may be formed between the container axis 105 and the first wall portion 122 of the second wall 102. The first wall portion 122 of the second wall 102 may be inclined at the angle α122 relative to the container axis 105. The first wall portion 122 of the second wall 102 is preferably inclined outwardly (away from the interior of the container 100) relative to the container axis 105.

[0145] An angle α132 may be formed between the container axis 105 and the second wall portion 132 of the second wall surface 102. The second wall portion 132 of the second wall surface 102 may be inclined (outwardly) at the angle α132 relative to the container axis 105.

[0146] An angle α142 may be formed between the container axis 105 and the third wall portion 142 of the second wall surface 102. The third wall portion 142 of the second wall surface 102 may be inclined (outwardly) at the angle α142 relative to the container axis 105.

[0147] The first wall portion 122 of the second wall surface 102, the second wall portion 132 of the second wall surface 102, and the third wall portion 142 of the second wall surface 102 may each be inclined (outwardly) with respect to the container axis 105.

[0148] Between the first wall portion 122 of the second wall surface 102 and the second wall portion 132 of the second wall surface 102, there may be a kink in the inclination of the walls 122, 132 with respect to the container axis 105. Also, between the second wall portion 132 of the second wall surface 102 and the third wall portion 142 of the second wall surface 102, there may be a kink in the inclination of the walls 132, 142 with respect to the container axis 105.

[0149] The angle α122 between the container axis 105 and the first wall 122 of the second wall 102 may be smaller than the angle α132 between the container axis 105 and the second wall 132 of the second wall 102. The angle α132 between the container axis 105 and the second wall 132 of the second wall 102 may be larger than the angle α142 between the container axis 105 and the third wall 142 of the second wall 102. The angle α122 between the container axis 105 and the first wall 122 of the second wall 102 may be smaller than the angle α142 between the container axis 105 and the third wall 142 of the second wall 102.

[0150] The fourth wall 104 may include a first wall portion 124 , a second wall portion 134 , and a third wall portion 144 .

[0151] The first wall 124 of the fourth wall 104 may be (directly) adjacent to the bottom 110, in particular in the direction of the container axis 105. The second wall 134 of the fourth wall 104 may be (directly) adjacent to the first wall 124 of the fourth wall 104, in particular in the direction of the container axis 105. The third wall 144 of the fourth wall 104 may be (directly) adjacent to the second wall 134 of the fourth wall 104, in particular in the direction of the container axis 105.

[0152] An angle α 124 may be formed between the container axis 105 and the first wall portion 124 of the fourth wall 104. The first wall portion 124 of the fourth wall 104 may be inclined at the angle α 124 relative to the container axis 105. The first wall portion 124 of the fourth wall 104 is preferably inclined outwardly (away from the interior of the container 100) relative to the container axis 105.

[0153] An angle α134 may be formed between the container axis 105 and the second wall portion 134 of the fourth wall surface 104. The second wall portion 134 of the fourth wall surface 104 may be inclined (outwardly) at the angle α134 relative to the container axis 105.

[0154] An angle α144 may be formed between the container axis 105 and the third wall portion 144 of the fourth wall surface 104. The third wall portion 144 of the fourth wall surface 104 may be inclined (outwardly) at the angle α144 relative to the container axis 105.

[0155] The first wall portion 124 of the fourth wall surface 104, the second wall portion 134 of the fourth wall surface 104, and the third wall portion 144 of the fourth wall surface 104 may each be inclined (outwardly) with respect to the container axis 105.

[0156] Between the first wall portion 124 of the fourth wall surface 104 and the second wall portion 134 of the fourth wall surface 104, there may be a kink in the inclination of the walls 124, 134 with respect to the container axis 105. Also, between the second wall portion 134 of the fourth wall surface 104 and the third wall portion 144 of the fourth wall surface 104, there may be a kink in the inclination of the walls 134, 144 with respect to the container axis 105.

[0157] The angle α124 between the container axis 105 and the first wall 124 of the fourth wall 104 may be smaller than the angle α134 between the container axis 105 and the second wall 134 of the fourth wall 104. The angle α134 between the container axis 105 and the second wall 134 of the fourth wall 104 may be larger than the angle α144 between the container axis 105 and the third wall 144 of the fourth wall 104. The angle α124 between the container axis 105 and the first wall 124 of the fourth wall 104 may be smaller than the angle α144 between the container axis 105 and the third wall 144 of the fourth wall 104.

[0158] The angle α122 between the container axis 105 and the first wall 122 of the second wall 102 may be substantially (±2% or ±1%) as high as the angle α124 between the container axis 105 and the first wall 124 of the fourth wall 104. The angle α132 between the container axis 105 and the second wall 132 of the second wall 102 may be substantially (±2% or ±1%) as high as the angle α134 between the container axis 105 and the second wall 134 of the fourth wall 104. The angle α142 between the container axis 105 and the third wall 142 of the second wall 102 may be substantially (±2% or ±1%) as high as the angle α144 between the container axis 105 and the third wall 144 of the fourth wall 104.

[0159] The angle α122 between the container axis 105 and the first wall portion 122 of the second wall 102 may be greater than the angle α121 between the container axis 105 and the first wall portion 121 of the first wall 101 .

[0160] The angle α 124 between the container axis 105 and the first wall portion 124 of the fourth wall 104 may be greater than the angle α 121 between the container axis 105 and the first wall portion 121 of the first wall 101 .

[0161] The angle α132 between the container axis 105 and the second wall portion 132 of the second wall surface 102 may be smaller than the angle α131 between the container axis 105 and the second wall portion 131 of the first wall surface 101 .

[0162] The angle α 134 between the container axis 105 and the second wall portion 134 of the fourth wall 104 may be smaller than the angle α 131 between the container axis 105 and the second wall portion 131 of the first wall 101 .

[0163] In Figures 5 and 6, a vessel 200 is shown. In Figure 5, the vessel 200 is shown in an isometric view, and in Figure 6, the vessel 200 is shown in a bottom view. In Figure 6, cross sections CC and DD are shown which are shown in Figures 7 and 8.

[0164] The container 200 may include a bottom 210 , a first wall 201 , a second wall 202 , a third wall 203 , and a fourth wall 204 .

[0165] The container 200 generally has a polygonal basic shape, and preferably the bottom 210 of the container 200 has a polygonal shape. The polygonal basic shape or polygonal shape may be rectangular.

[0166] The container 200, and in particular the bottom 210 of the container 200, may include markings 211, 212, 213, 214. Each of the marks 211, 212, 213, 214 may be assigned to one of the walls 201, 202, 203, 204. The marks 211, 212, 213, 214 may be used to identify the walls 201, 202, 203, 204. For example, the marks 211, 212, 213, 214 may be consecutive numbers, e.g. 1, 2, 3, 4, or the marks 211, 212, 213, 214 may be consecutive letters, e.g. A, B, C, D.

[0167] FIG. 7 shows a cross section CC where the bottom 210, the first wall 201 and the third wall 203 are cross-sectioned.

[0168] The inner surface of bottom 210 (facing the interior of container 200) may be flat. Bottom 210 may be provided with an injection dome 218 such that the presence of injection dome 218 results in the inner surface of bottom 210 being flat. Injection dome 218 may be provided during injection molding of container 100.

[0169] The bottom 210, for example an inner surface of the bottom 210, may define a container axis 205. The container axis 205 may extend perpendicular to the bottom 210. The bottom 210 may define a plane perpendicular to the container axis 205.

[0170] The first wall surface 201 may include a first wall portion 221 , a second wall portion 231 , and a third wall portion 241 .

[0171] The first wall 221 may be (directly) adjacent to the bottom 210, in particular in the direction of the container axis 205. The second wall 231 may be (directly) adjacent to the first wall 221, in particular in the direction of the container axis 205. The third wall 241 may be (directly) adjacent to the second wall 231, in particular in the direction of the container axis 205.

[0172] An angle α221 may be formed between the container axis 205 and the first wall 221. The first wall 221 may be inclined at this angle α221 relative to the container axis 205. The first wall 221 is preferably inclined outwardly (away from the interior of the container 200) relative to the container axis 205.

[0173] An angle α231 may be formed between the container axis 205 and the second wall 231. The second wall 231 may be inclined (outwardly) at the angle α231 relative to the container axis 205.

[0174] An angle α241 may be formed between the container axis 205 and the third wall 241. The third wall 241 may be inclined (outwardly) at the angle α241 relative to the container axis 205.

[0175] The first wall portion 221, the second wall portion 231, and the third wall portion 241 may each be inclined (outwardly) with respect to the container axis 205.

[0176] The cross section of the container 200 at the bottom 210 (cross section parallel to the bottom 210) may be smaller than the cross section of the container 200 at the first wall 221 (cross section at the height of the central part of the first wall 221 in the direction of the container axis 205). The cross section of the container 200 at the first wall 221 (cross section at the height of the central part of the first wall 221 in the direction of the container axis 205) may be smaller than the cross section of the container 200 at the second wall 231 (cross section at the height of the central part of the second wall 231 in the direction of the container axis 205). The cross section of the container 200 at the second wall 231 (cross section at the height of the central part of the second wall 231 in the direction of the container axis 205) may be smaller than the cross section of the container 200 at the third wall 241 (cross section at the height of the central part of the third wall 241 in the direction of the container axis 205).

[0177] Between the first wall 221 and the second wall 231, there may be a kink in the inclination of the walls 221, 231 relative to the container axis 205. The kink may result from an abrupt or discontinuous change in the inclination relative to the container axis 205. Between the second wall 231 and the third wall 241, there may be a kink in the inclination of the walls 231, 241 relative to the container axis 205.

[0178] The angle α221 between the container axis 205 and the first wall 221 may be smaller than the angle α231 between the container axis 205 and the second wall 231. The angle α231 between the container axis 205 and the second wall 231 may be larger than the angle α241 between the container axis 205 and the third wall 241. The angle α221 between the container axis 205 and the first wall 221 may be smaller than the angle α241 between the container axis 205 and the third wall 241.

[0179] The third wall 203 may include a first wall portion 223 , a second wall portion 233 , and a fourth wall portion 243 .

[0180] The first wall portion 223 of the third wall surface 203 may be (directly) adjacent to the bottom portion 210. The second wall portion 233 of the third wall surface 203 may be (directly) adjacent to the first wall portion 233 of the third wall surface 203. The third wall portion 243 of the third wall surface 203 may be (directly) adjacent to the second wall portion 233 of the third wall surface 203.

[0181] The first wall portion 221 of the first wall surface 201 faces the first wall portion 223 of the third wall surface 203. The first wall portion 221 of the first wall surface 201 may have substantially the same height (±10%) as the first wall portion 223 of the third wall surface 203.

[0182] The second wall portion 231 of the first wall surface 201 may face the second wall portion 233 of the third wall surface 203. The second wall portion 231 of the first wall surface 201 may have substantially the same height as the second wall portion 233 of the third wall surface 203 (±10%).

[0183] The third wall portion 241 of the first wall surface 201 may face the third wall portion 243 of the third wall surface 203. The third wall portion 241 of the first wall surface 201 may have substantially the same height as the third wall portion 243 of the third wall surface 203 (±10%).

[0184] The slope of the first wall portion 223 of the third wall surface 203 may correspond to or be equal to the slope of the first wall portion 221 of the first wall surface 201. The slope of the second wall portion 233 of the third wall surface 203 may correspond to or be equal to the slope of the second wall portion 231 of the first wall surface 201. The slope of the third wall portion 243 of the third wall surface 203 may correspond to or be equal to the slope of the third wall portion 241 of the first wall surface 201.

[0185] Generally, the third wall 203 corresponds to the first wall 201 such that the third wall 203 mirrors the first wall 201 with respect to the container axis 205. Generally, the container axis 205 is formed in the center of the base 210.

[0186] FIG. 8 shows cross section DD shown in FIG. 6, with the bottom 210, the second wall 202, and the fourth wall 204 cross-sectioned.

[0187] The second wall 202 may include a first wall portion 222 , a second wall portion 232 , and a third wall portion 242 .

[0188] The first wall portion 222 of the second wall surface 202 may have characteristics of the first wall portion 221 of the first wall surface 201. The second wall portion 232 of the second wall surface 202 may have characteristics of the second wall portion 231 of the first wall surface 201. The third wall portion 242 of the second wall surface 202 may have characteristics of the third wall portion 241 of the first wall surface 201.

[0189] The fourth wall 204 may include a first wall portion 224 , a second wall portion 234 , and a third wall portion 244 .

[0190] The first wall portion 224 of the fourth wall surface 204 may have characteristics of the first wall portion 221 of the first wall surface 201. The second wall portion 234 of the fourth wall surface 204 may have characteristics of the second wall portion 231 of the first wall surface 201. The third wall portion 244 of the fourth wall surface 204 may have characteristics of the third wall portion 241 of the first wall surface 201.

[0191] In general, the first wall 201 of the container 200 may have the characteristics of the first wall 101 of the container 100. The second wall 202 of the container 200 may have the characteristics of the second wall 102 of the container 100. The third wall 203 of the container 200 may have the characteristics of the third wall 103 of the container 100. The fourth wall 204 of the container 200 may have the characteristics of the fourth wall 104 of the container 100. Similarly, the walls 101, 102, 103, 104 of the container 100 may have the characteristics of the walls 201, 202, 203, 204 of the container 200, respectively.

[0192] Generally, a larger angle or tilt than another angle or tilt is at least 1°, 2°, 3°, or 5° larger. Generally, a smaller angle or tilt than another angle or tilt is at least 1°, 2°, 3°, or 5° smaller.

[0193] Figure 9 shows a system 400 for measuring a sample 320. The sample 320 can be placed in a container 100. Although the container 100 is shown in Figure 9 as an example, the container 200 can also be used.

[0194] The sample 320 may have a height (in the vessel axial direction) of 2 mm or more, preferably 5 mm or more, more preferably 10 mm or more, more preferably 20 mm or more.

[0195] A liquid 330 can be introduced into the container 100. The liquid 330 can completely cover the sample 320. The surface of the liquid 330 can be curved at the contact with the wall of the container 100. At the contact, a meniscus 331, 332 is formed. The liquid 330 can be introduced into the container 100 such that the surface of the liquid 330 parallel to the cross section of the first wall or walls of the container 100 is substantially (±5°) planar. The cross section of the first wall or walls of the container can be projected onto the surface of the liquid 330. The area of ​​the surface of the liquid 330 can therefore be considered to have the same dimensions as the cross section of the first wall.

[0196] The system may include a radiation source 300. The radiation source 300 may be a laser. The radiation source 300 may irradiate the sample 320. The sample 320 is preferably irradiated by the radiation source 300 through a first wall of the container 100. Luminescence in the sample 320 may be excited by the irradiation. The sample 320 may be irradiated through one of the walls of the container, or through at least two of the walls, or through all of the walls. Different walls may be irradiated sequentially. One or more walls may be irradiated simultaneously.

[0197] The system 400 may include a radiation receiver 350. The radiation receiver 350 may be a camera. The radiation receiver 350 may record the emission of the sample 320. The radiation source 300, the sample 320 and the radiation receiver 350 are preferably not in a (straight) line. The radiation receiver 350 may be arranged such that the emission of the sample 320 can be recorded or is recorded substantially perpendicular (±10°) to the direction of irradiation of the sample 320 by the radiation source 300. The radiation receiver 350 may be arranged such that the emission of the sample 320 can be received or is received substantially perpendicular (±10°) to the bottom of the container 100.

[0198] An objective lens may be disposed between the sample 320 and the radiation receiver 350 .

[0199] The objective lens may be at least partially immersed in the liquid 330 during irradiation or measurement of the sample 320. Alternatively, the lens may be positioned above the liquid surface during irradiation of the sample 320.

[0200] The opening of the container 100 may be covered during irradiation of the sample 320, for example by one or more glass plates.

[0201] For example, the radiation source 300 can irradiate the sample 320 through the first wall of the container 100. Because the first wall has a slope of 1.9°±0.7° or n times thereof, and because of the refractive index of the interfaces on the path of the radiation from the radiation source 300 to the sample 320, the radiation from the radiation source 330 can be offset at an angle (integer) such that the radiation is incident on the sample 320 parallel to the bottom, and parallel to the liquid surface if the sample 320 is covered with liquid. At the same time, the container can be manufactured cheaply using an injection molding process (draft angle).

[0202] The first wall portion of the third wall of the container 100 is inclined relative to the first wall portion of the wall where irradiation is performed, thereby making it possible to avoid diffuse reflection. In particular, in the case of a small sample that is completely penetrated by the irradiation of the radiation source 330, if the first wall portion of the third wall is not inclined relative to the first wall portion of the wall where irradiation is performed, the radiation may be reflected in the direction of the sample 330.

[0203] The rectangular (non-square) basic shape of the container 100 or the bottom 110 of the container 100 may correspond to the optimal thin portion (Rayleigh length) of the light sheet (radiation source 300) along the irradiation direction and the irradiation field of the objective lens in the direction perpendicular to the irradiation direction.

[0204] Numbered exemplary embodiments are disclosed below. 1. A container (100, 200) for a sample (320), comprising a bottom (110, 210) perpendicular to a container axis (105, 205), a first wall (121, 221) adjacent to the bottom (110, 210), and a second wall (131, 231) adjacent to the first wall (110, 210), wherein the second wall (131, 231) is inclined at an angle of 20° or more relative to the container axis (105, 205).

[0205] 2. A container (100, 200) according to embodiment 1, wherein the first wall portion (121, 221) is inclined at an angle of 0.5° or more, preferably 1.0° or more, particularly preferably 1.5° or more, relative to the container axis (105, 205), and / or the first wall portion (121, 221) is inclined at an angle of 25° or less, preferably 10° or less, particularly preferably 5° or less, relative to the container axis (105, 205).

[0206] 3. A container (100, 200) according to embodiment 1 or 2, wherein the container (100, 200) includes a third wall portion (141, 241) adjacent to the second wall portion (131, 231), and the third wall portion (141, 241) is inclined at an angle of 2° or more relative to the container axis (105, 205).

[0207] 4. A container (100, 200) according to any of the embodiments 1 to 3, wherein the container (100, 200) has a polygonal, in particular rectangular or quadrangular, basic shape and has a first wall (101, 201), a second wall (102, 202), a third wall (103, 203), and a fourth wall (104, 204).

[0208] 5. A container (100) according to example 4, wherein the first wall surface (101) includes first, second and third wall portions (121, 131, 141) and the third wall surface (103) includes first, second and third wall portions (123, 133, 143), and the first wall portion (123) of the third wall surface (103) has a greater inclination with respect to the container axis (105) than the first wall portion (121) of the first wall surface (101), in particular, the first wall portion (123) of the third wall surface (103) has a greater inclination with respect to the container axis (105) than the first wall portion (121) of the first wall surface (101) by at least 2°, preferably by at least 5°.

[0209] 6. A container (100) according to embodiment 4 or 5, wherein the first wall surface (101) comprises a first, second and third wall portion (121, 131, 141) and the third wall surface (103) comprises a first, second and third wall portion (123, 133, 143), and the second wall portion (133) of the third wall surface (103) has a smaller inclination relative to the container axis (105) than the second wall portion (131) of the first wall surface (101), in particular, the second wall portion (133) of the third wall surface (103) has a smaller inclination relative to the container axis (105) than the second wall portion (131) of the first wall surface (101) by at least 2°, preferably by at least 5°.

[0210] 7. The first wall (101) comprises first, second and third wall portions (121, 131, 141), the second and / or fourth wall (102, 104) comprise first, second and third wall portions (122, 124, 132, 134, 142, 144), respectively, and the first wall portions (122, 124) of the second wall (102) and / or the fourth wall (104) are aligned with the container axis (105). The container (100) according to any of Examples 4 to 6, wherein the first wall portion (121) of the first wall surface (101) has a greater inclination with respect to the container axis (105), and in particular the first wall portions (122, 124) of the second wall surface (102) and / or the fourth wall surface (104) have an inclination that is greater than or equal to 1°, preferably greater than or equal to 2°, with respect to the container axis (105).

[0211] 8. The first wall (101) comprises first, second and third wall portions (121, 131, 141), the second and / or fourth wall (102, 104) comprise first, second and third wall portions (122, 124, 132, 134, 142, 144), respectively, and the second wall portions (132, 134) of the second wall (102) and / or the fourth wall (104) are aligned with the container axis (105). The container (100) according to any of Examples 4 to 7, wherein the second wall portion (132, 134) of the second wall surface (102) and / or the fourth wall surface (104) has a smaller inclination relative to the container axis (105) than the second wall portion (131) of the first wall surface (101), and in particular the second wall portions (132, 134) of the second wall surface (102) and / or the fourth wall surface (104) have an inclination that is smaller by at least 1°, preferably by at least 2°, than the second wall portion (131) of the first wall surface (101).

[0212] 9. The container (200) according to embodiment 4, wherein each of the first, second, third and fourth walls (201, 202, 203, 204) comprises a first wall portion (221) and / or a second wall portion (231) and / or a third wall portion (241).

[0213] 10. A container (100, 200) for a sample (320), comprising a bottom (110, 210) perpendicular to a container axis (105, 205) and a first wall (121, 221) adjacent to the bottom (110, 210), wherein the first wall (121, 221) is inclined at an angle of 1.9°±0.7° or n multiples thereof, where n is an integer greater than 1, relative to the container axis (105, 205).

[0214] 11. The container (100, 200) according to embodiment 10, wherein the first wall (121, 221) is inclined at an angle of 1.9°±0.5°, in particular 1.9°±0.3° or a multiple n thereof (n being an integer greater than 1) relative to the container axis (105, 205).

[0215] 12. The container (100, 200) according to any of the embodiments 1 to 11, wherein the container (100, 200) is an injection molded container (100, 200) and / or the container (100, 200) comprises or consists of a thermoplastic resin and / or the container (100, 200) comprises or consists of a block copolymer, in particular comprises or consists of a cyclic block copolymer.

[0216] 13. At least a portion of the container (100, 200) has a density measured according to ASTM D792 of 1.00 g / cm 3and / or a water absorption measured in accordance with ASTM D570 of less than 5%, preferably less than 2.0%, more preferably less than 0.5%, and / or a melt flow rate measured in accordance with ASTM D1238 at 2.6 kg and 260°C of 1.0 cm / 10 min or more, preferably 7.0 cm / 10 min or more, more preferably 100. and / or a transmittance at 3 mm measured according to ASTM D1003 of 50% or more, preferably 70% or more, more preferably 85% or more, more preferably 90% or more; a haze at 3 mm measured according to ASTM D1003 of less than 5.0%, preferably less than 3.0%, more preferably less than 1.5%, more preferably less than 1.0%; and / or a reflectance of less than 5.0, preferably and / or a Vicat softening point measured at 1 kg and 50°C / h according to ASTM D1525 of 70°C or more, preferably 85°C or more, more preferably 100°C or more, more preferably 90°C to 130°C, and / or a heat deflection temperature measured at 0.455 MPa and 2°C / min according to ASTM D648 of 50°C or more, preferably 60°C or more, more preferably 75°C or more, more preferably 60°C to 115°C, and / or a flexural strength measured according to ASTM D790 of 30 MPa or more, preferably 40 MPa or more, more preferably 50 MPa or more, more preferably 40 MPa to 80 MPa, and / or a flexural modulus measured according to ASTM D790 of 600 MPa or more, preferably 1000 MPa or more, more preferably 1400 MPa or more, more preferably 1000 MPa to 3000 MPa, and / or a tensile strength, YP or BP measured according to ASTM D638.and / or a strain measured according to ASTM D638 of 5% or more, preferably 8% or more, more preferably 10% or more, more preferably 8% to 40%; and / or an Izod impact strength measured according to ASTM D256 of 1 kgcm / cm or more, preferably 1.5 kgcm / cm or more, more preferably 2.0 kgcm / cm or more, more preferably 1.5 kgcm / cm to 5.0 kgcm / cm; and / or methyl salicylate. A container (100, 200) according to any one of Examples 1 to 12, made of a material having one or more of the following characteristics: chemical resistance to dibenzyl ether, a mixture of 1 part benzyl alcohol and 2 parts benzyl benzoate, a mixture of 4 parts benzyl benzoate and 1 part diphenyl ether, ethyl cinnamate, and / or 2,2'-thiodiethanol, and / or an autofluorescence of 400 nm to 460 nm with an excitation light of 350 nm and a sample thickness of 1 mm is 50% or less, preferably 40% or less, more preferably 30% or less, and more preferably 25% or less, relative to the autofluorescence of polystyrene.

[0217] 14. A method for testing a sample (320), in particular a biological sample, comprising the steps of placing the sample (320) in a container (100, 200) according to any of the embodiments 1 to 13, irradiating the sample (320) and recording the luminescence of the sample.

[0218] 15. Use of a container (100, 200) according to any of Examples 1 to 13 for containing a sample (320) during examination of the sample (320), preferably during microscopy, more preferably during light sheet microscopy, more preferably during light sheet fluorescence microscopy.

Claims

1. A container (100, 200) for a sample (320), a bottom (110, 210) perpendicular to the vessel axis (105, 205); a first wall (121, 221) adjacent to said bottom (110, 210), said first wall (121, 221) forming an angle between said first wall (121, 221) and said container axis (105, 205) of between 0.5° and 50°; a second wall (131, 231) adjacent to the first wall (110, 210), the second wall (131, 231) being inclined at an angle to the vessel axis (105, 205), the angle between the second wall (131, 231) and the vessel axis (105, 205) being between 30° and 80°; a third wall (141, 241) adjacent to the second wall (131, 231), the third wall (141, 241) being inclined at an angle to the container axis (105, 205), the angle between the third wall (141, 241) and the container axis (105, 205) being between 2° and 30°; Including, The container (100, 200) has a polygonal basic shape.

2. A container (100, 200) as described in claim 1, wherein the angle between the first wall portion (121, 221) and the container axis (105, 205) is 0.5° to 30°, preferably 0.5° to 10°.

3. The container (100, 200) according to claim 1 or 2, wherein the container (100, 200) has a first wall (101, 201), a second wall (102, 202), a third wall (103, 203) and a fourth wall (104, 204).

4. The first wall surface (101) includes the first, second and third wall portions (121, 131, 141), The third wall surface (103) includes the first, second and third wall portions (123, 133, 143), The container (100) of claim 3, wherein the first wall portion (123) of the third wall surface (103) has a greater inclination with respect to the container axis (105) than the first wall portion (121) of the first wall surface (101), in particular, the first wall portion (123) of the third wall surface (103) has a greater inclination with respect to the container axis (105) than the first wall portion (121) of the first wall surface (101) by at least 2°, preferably by at least 5°.

5. The first wall surface (101) includes the first, second and third wall portions (121, 131, 141), The third wall surface (103) includes the first, second and third wall portions (123, 133, 143), 5. The container (100) according to claim 3 or 4, wherein the second wall portion (133) of the third wall surface (103) has a smaller inclination with respect to the container axis (105) than the second wall portion (131) of the first wall surface (101), in particular, the second wall portion (133) of the third wall surface (103) has a smaller inclination with respect to the container axis (105) than the second wall portion (131) of the first wall surface (101) by at least 2°, preferably by at least 5°.

6. The first wall surface (101) includes the first, second and third wall portions (121, 131, 141), the second and / or fourth wall surfaces (102, 104) respectively include the first, second and third wall portions (122, 124, 132, 134, 142, 144); The container (100) according to any one of claims 3 to 5, wherein the first wall portion (122, 124) of the second wall surface (102) and / or the fourth wall surface (104) has a greater inclination with respect to the container axis (105) than the first wall portion (121) of the first wall surface (101), in particular the first wall portion (122, 124) of the second wall surface (102) and / or the fourth wall surface (104) has a greater inclination with respect to the container axis (105) than the first wall portion (121) of the first wall surface (101) by at least 1°, preferably by at least 2°.

7. The first wall surface (101) includes the first, second and third wall portions (121, 131, 141), the second and / or fourth wall surfaces (102, 104) respectively include the first, second and third wall portions (122, 124, 132, 134, 142, 144); The container (100) according to any one of claims 3 to 6, wherein the second wall portion (132, 134) of the second wall surface (102) and / or the fourth wall surface (104) has a smaller inclination with respect to the container axis (105) than the second wall portion (131) of the first wall surface (101), in particular the second wall portion (132, 134) of the second wall surface (102) and / or the fourth wall surface (104) has a smaller inclination with respect to the container axis (105) than the second wall portion (131) of the first wall surface (101) by at least 1°, preferably by at least 2°.

8. The container (200) of claim 3, wherein each of the first, second, third and fourth walls (201, 202, 203, 204) comprises the first wall portion (221) and / or the second wall portion (231) and / or the third wall portion (241).

9. A container (100, 200) for a sample (320), a bottom (110, 210) perpendicular to the vessel axis (105, 205); a first wall portion (121, 221) adjacent to the bottom portion (110, 210); the first wall portion (121, 221) is inclined at an angle of 1.9°±0.3° or n multiples thereof (n being an integer greater than 1) with respect to the container axis (105, 205); The container has a polygonal basic shape (100, 200).

10. said container (100, 200) is an injection molded container (100, 200) and / or said container (100, 200) comprises or consists of a thermoplastic resin and / or The container (100, 200) according to any one of claims 1 to 9, comprising or consisting of a block copolymer, in particular comprising or consisting of a cyclic block copolymer.

11. At least a portion of the container (100, 200) The density, measured according to ASTM D792, is 1.00 g / cm ３ or less, preferably 0.98 g / cm ３ or less, more preferably 0.96 g / cm ３ Particularly preferably 0.92 g / cm ３ ~0.96g / cm ３ and / or a water absorption measured according to ASTM D570 of less than 5%, preferably less than 2.0%, more preferably less than 0.5%; and / or The melt flow rate measured at 260°C under 2.6 kg in accordance with ASTM D1238 is 1.0 cm ３ / 10 minutes or more, preferably 7.0 cm ３ / 10 minutes or more, more preferably 100.0 cm ３ / 10 minutes or more, more preferably 2.0 cm ３ / 10 minutes to 250.0cm ３ / 10 minutes, and / or The transmittance at 3 mm measured according to ASTM D1003 is 50% or more, preferably 70% or more, more preferably 85% or more, more preferably 90% or more; a haze at 3 mm measured according to ASTM D1003 of less than 5.0%, preferably less than 3.0%, more preferably less than 1.5%, more preferably less than 1.0%, and / or a reflectance of less than 5.0, preferably less than 3.0, more preferably less than 2.5; and / or a Vicat softening point, measured according to ASTM D1525 at 1 kg and 50° C. / h, of 70° C. or more, preferably 85° C. or more, more preferably 100° C. or more, more preferably 90° C. to 130° C.; and / or a heat deflection temperature measured according to ASTM D648 at 0.455 MPa and 2°C / min of 50°C or more, preferably 60°C or more, more preferably 75°C or more, more preferably from 60°C to 115°C; and / or A flexural strength measured according to ASTM D790 of 30 MPa or more, preferably 40 MPa or more, more preferably 50 MPa or more, more preferably 40 MPa to 80 MPa; and / or A flexural modulus measured according to ASTM D790 of 600 MPa or more, preferably 1000 MPa or more, more preferably 1400 MPa or more, more preferably 1000 MPa to 3000 MPa; and / or Tensile strength, Y.P. or B.P., measured according to ASTM D638, is 15 MPa or more, preferably 25 MPa or more, more preferably 30 MPa or more, more preferably 20 MPa to 50 MPa; and / or a distortion measured according to ASTM D638 of 5% or more, preferably 8% or more, more preferably 10% or more, more preferably 8% to 40%, and / or and / or an Izod impact strength measured according to ASTM D256 of 1 kgcm / cm or more, preferably 1.5 kgcm / cm or more, more preferably 2.0 kgcm / cm or more, more preferably 1.5 kgcm / cm to 5.0 kgcm / cm; and / or Chemical resistance to methyl salicylate, dibenzyl ether, a mixture of 1 part benzyl alcohol and 2 parts benzyl benzoate, a mixture of 4 parts benzyl benzoate and 1 part diphenyl ether, ethyl cinnamate, and / or 2,2'-thiodiethanol, and / or The autofluorescence of 400 nm to 460 nm with a sample thickness of 1 mm and excitation light of 350 nm is 50% or less, preferably 40% or less, more preferably 30% or less, more preferably 25% or less, relative to the autofluorescence of polystyrene. The container (100, 200) according to any one of claims 1 to 10, which is made of a material having one or more of the following characteristics:

12. A method for testing a sample (320), in particular a biological sample, comprising: - introducing a sample (320) into a container (100, 200) according to any one of claims 1 to 11; irradiating the sample (320); and recording the luminescence of the sample, A method, wherein the emission of the sample (320) is recorded substantially perpendicular to the direction of illumination.

13. Use of a container (100, 200) according to any one of claims 1 to 11 for containing a sample (320) during light sheet microscopy, preferably light sheet fluorescence microscopy, of said sample (320).

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