Prevention / therapeutic agent of allergic dermatitis

Abstract

To provide a novel prevention / therapeutic agent of allergic dermatitis.SOLUTION: Technical features of the present invention in order to solve the above problem are as follows. 1. inhibitor of itching caused by immediate-type allergy which has lycoperosides and / or tomato seed extract as active ingredients. 2. the inhibitor of itching caused by immediate-type allergy described in 1 above in which the lycoperosides is lycoperoside H. 3. reaction inhibitor for immediate-type allergy which has lycoperoside H and / or tomato seed extract as active ingredients.SELECTED DRAWING: None

Description

[Technical field]

[0001] The present invention relates to a preventive and therapeutic agent for allergic dermatitis. The present invention is widely used in foods, medicines, cosmetics, external skin preparations, etc. [Background technology]

[0002] Tomato (Solanum lycopersicum) is a plant that is eaten daily around the world, and is sold as juice and supplements because it is rich in vitamin C, lycopene, GABA, and other ingredients. The functionality of tomatoes has been reported to include blood pressure lowering and cholesterol lowering effects. In addition, in 2004, esculeosides, which are novel saponins, were identified from tomatoes (Non-Patent Documents 1 and 2), and esculeoside A was found to have the effect of improving hyperlipidemia and arteriosclerosis in mice (Non-Patent Document 3). However, these findings are all from tomato fruits, and there are few reports limited to tomato seeds.

[0003] In addition, as dry skin is a typical symptom of allergic dermatitis, maintaining the moisture content of the skin leads to alleviation of allergic dermatitis symptoms. Several moisturizing factors are active in the skin, representative of which are filaggrin and ceramide. It is known that these are significantly reduced in the skin of people with atopic dermatitis, and it is believed that increasing the filaggrin and ceramide content in the skin can alleviate dry skin, a symptom of allergic dermatitis.

[0004] The biosynthetic pathway of sphingolipids has been well studied. First, in the very first step of sphingolipid synthesis, the non-essential amino acid L-serine and palmitoyl CoA are used as starting materials, and palmitoyl CoA and the non-essential amino acid L-serine undergo condensation polymerization by serine palmitoyltransferase (SPT), and ceramide is synthesized in the spinous layer through four steps. Then, sphingomyelin synthase converts it to sphingomyelin, which accumulates in the granular layer, and then acid sphingomyelinase synthesizes ceramide again in the stratum corneum (pathway on the right in Figure 6).

[0005] In addition, ceramide synthesized in the spinous layer is converted to glucosylceramide by glucosylceramide synthase and accumulates in the granular layer, and then ceramide is synthesized again in the stratum corneum by glucocerebrosidase (left pathway in Figure 6).

[0006] Furthermore, there are 10 types of ceramides (EOS, NS, NP, EOH, AS, AP, AH, NH, EOP), and it is said that a deficiency of ceramides (EOS), (NP), and (AP) in particular has a strong impact on dry skin, sensitive skin, and atopic dermatitis.

[0007] Here, ceramides (EOS, NS, EOH, AS, AP) are produced via the left pathway in Figure 6, which produces ceramide from glucosylceramide. In contrast, the right pathway, which produces ceramide from sphingomyelin, produces only ceramides (NS) and (AS). In particular, ceramide NP, which is involved in alleviating dry skin caused by allergic skin inflammation and the like, is produced only via the left pathway (Patent Document 1).

[0008] [Non-Patent Document 1] Fujiwara S. et al., Tetrahedron, 60, 4915-4920 (2004). [Non-Patent Document 2] Ono M. et al., Chem. Pharm. Bull., 54(2), 237-239 (2006). [Non-Patent Document 3] Nohara T., J. Trad. Med., 27, 217-224 (2010). [Patent Document 1] JP 2017-105741 A DISCLOSURE OF THEINVENTION [Problem to be solved by the invention]

[0009] Under the above circumstances, the present inventors evaluated the effectiveness of tomato seed extract and lycoperosides against allergic dermatitis and found that they have an effect of improving allergic skin. They also found that tomato seed extract and lycoperosides have an effect of promoting filaggrin expression and a moisturizing effect, thus completing the present invention. That is, an object of the present invention is to provide a novel agent for preventing and treating allergic dermatitis. [Means for solving the problem]

[0010] The technical features of the present invention for solving the above problems are as follows. 1. An agent for suppressing itching caused by immediate-type allergies, containing lycoperosides and / or tomato seed extract as active ingredients. 2. The agent for suppressing itching caused by immediate-type allergy according to 1 above, wherein the lycoperoside is lycoperoside H. 3. An immediate-type allergic reaction suppressant containing lycoperoside H and / or tomato seed extract as active ingredients. 4. A moisturizer containing lycoperosides and / or tomato seed extract as active ingredients. 5. A filaggrin gene expression promoter having lycoperosides and / or tomato seed extract as active ingredients. 6. A ceramide synthase-3 expression promoter having lycoperosides and / or tomato seed extract as active ingredients. 7. The agent according to any one of 4. to 6. above, wherein the lycoperosides are at least one of lycoperoside A and lycoperoside H. 8. A GCS gene expression promoter comprising at least one of tomato seed extract, lycoperoside A, and lycoperoside H as an active ingredient. 9. A GBA gene expression promoter comprising at least one of tomato seed extract, lycoperoside A, and lycoperoside H as an active ingredient. 10. An SMS gene expression inhibitor comprising at least one of tomato seed extract and lycoperoside H as an active ingredient. 11. Ceramide NP production promoter containing lycoperoside H as an active ingredient. 12. A preventive and / or therapeutic agent for allergic dermatitis, comprising any one of the agents described in 1. to 11. above as an active ingredient. 13. A preventive and therapeutic agent for allergic dermatitis, the active ingredients of which are lycoperosides and / or tomato seed extract. Effect of the Invention

[0011] According to the present invention, lycoperosides, which are saponins found only in tomato seeds and not in fruit pulp, and tomato seed extracts have the effect of suppressing itching caused by immediate-type allergies, making these ingredients useful as preventive and therapeutic agents for allergic dermatitis. Furthermore, among the above lycoperosides, lycoperoside H is particularly advantageous in suppressing itching caused by immediate-type allergies. Furthermore, lycoperoside H and / or tomato seed extract have the effect of suppressing immediate-type allergies. In addition, lycoperosides and tomato seed extract have moisturizing effects, filaggrin gene expression promoting effects, and ceramide synthase-3 expression promoting effects, and therefore can be used as an agent for preventing and treating allergic dermatitis. Furthermore, lycoperosides and / or tomato seed extract not only suppress immediate-type allergies, but also have an effect of improving dermatitis caused by dry skin, and therefore are useful as an agent for improving dermatitis caused by dry skin. This promotes the production of ceramide in the pathway on the left in Figure 6, thereby promoting the production of ceramide EOS, NP, and AP, especially ceramide NP, which are said to be particularly effective in preventing dry skin, sensitive skin, atopic dermatitis, etc. As described above, in the present invention, lycoperosides and tomato seed extract are useful as agents for preventing and treating allergic dermatitis. [Brief description of the drawings]

[0012] [Figure 1] 1 is a graph showing the effects of a tomato seed extract and lycoperoside H on a mouse pruritus model. [Diagram 2] 1 is a graph showing the effects of a tomato seed extract and lycoperoside H on mouse ear PCA reactions. [Diagram 3] 1 is a graph showing the effects of tomato seed extract and lycoperosides on filaggrin gene expression. [Figure 4] 1 is a graph showing the effects of tomato seed extract and lycoperosides on ceramide synthase-3 (CerS3) gene expression. [Diagram 5] 1 is a graph showing the effects of a tomato seed extract and lycoperosides on TEWL. [Figure 6] 1 shows the effect of tomato seed extract on the stratum corneum ceramide synthesis pathway, with the white arrow indicating the effect of tomato seed extract. [Figure 7]1 is a graph showing the effects of tomato seed extract and lycoperosides on GCS gene expression. [Figure 8] 1 is a graph showing the effects of tomato seed extract and lycoperosides on GBA gene expression. [Figure 9] 1 is a graph showing the effects of tomato seed extract and lycoperosides on SMS gene expression. [Figure 10] 1 is a graph showing the effects of tomato seed extract and lycoperosides on ASM gene expression. [Figure 11] 1 is a graph showing the effects of tomato seed extract and lycoperosides on the amount of ceramide in the stratum corneum. BEST MODE FOR CARRYING OUT THEINVENTION

[0013] The present invention will be described in detail below. The preventive and therapeutic agent for allergic dermatitis of the present invention is characterized in that it contains lycoperosides as an active ingredient. The lycoperosides are not particularly limited, but examples thereof include lycoperoside A and lycoperoside H. These may be used alone or in combination of two or more kinds. Lycoperoside A is a saponin represented by the following chemical formula (1). [ka] Lycoperoside H is a saponin represented by the following chemical formula (2). [ka]

[0014] The method for obtaining lycoperoside A and lycoperoside H is not particularly limited, but a method of extracting and purifying them from plants is particularly preferred. When lycoperoside A and lycoperoside H are extracted from a plant, the raw material is not particularly limited, but it is particularly preferable to use tomato, since it contains lycoperoside A and lycoperoside H at high concentrations. When lycoperoside A and lycoperoside H are obtained from tomatoes, tomato seeds are used as the part of the plant that contains them, because lycoperoside A and lycoperoside H are not contained in the flesh or skin of tomatoes, but are contained only in tomato seeds.

[0015] Furthermore, when solvent extraction is used as the extraction method, examples of the extraction solvent for obtaining the extract include water, lower monohydric alcohols (methyl alcohol, ethyl alcohol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, etc.), liquid polyhydric alcohols (glycerin, propylene glycol, 1,3-butylene glycol, etc.), lower esters (ethyl acetate, etc.), hydrocarbons (benzene, hexane, pentane, etc.), ketones (acetone, methyl ethyl ketone, etc.), ethers (diethyl ether, tetrahydrofuran, dipropyl ether, etc.), acetonitrile, etc., and one or more of these can be used.

[0016] A preferred example of the extraction method is a method in which extraction is performed using aqueous ethanol or aqueous methanol at a concentration of 0 to 100% (v / v) at room temperature or with heating for 1 to 10 hours, followed by filtration.

[0017] As a purification method, the tomato seed extract obtained by the above method can be subjected to sequential partition extraction with ethyl acetate and n-butanol, and then silica gel chromatography, column chromatography, etc. to obtain lycoperoside A and lycoperoside H.

[0018] As a specific method for obtaining lycoperoside A and lycoperoside H from tomato seeds, the method according to the Examples of this specification is preferable.

[0019] The allergic dermatitis preventive and therapeutic agent of the present invention can be used as a material for various food and beverage compositions. Examples of food and beverage include general foods such as confectionery (gum, candy, caramel, chocolate, cookies, snacks, jellies, gummies, tablets, etc.), noodles (buckwheat, udon, ramen, etc.), dairy products (milk, ice cream, yogurt, etc.), seasonings (miso, soy sauce, etc.), soups, beverages (juice, coffee, black tea, green tea, carbonated drinks, sports drinks, etc.), health foods (tablets, capsules, etc.), nutritional supplements (nutritional drinks, etc.), functional foods, and foods for specified health uses. The allergic dermatitis preventive and therapeutic agent of the present invention may be appropriately blended into these foods and beverages.

[0020] These food and beverage compositions can contain various ingredients depending on their type, and examples of food ingredients that can be used include glucose, fructose, sucrose, maltose, sorbitol, stevioside, corn syrup, lactose, citric acid, tartaric acid, malic acid, succinic acid, lactic acid, L-ascorbic acid, dl-α-tocopherol, sodium erythorbate, glycerin, propylene glycol, glycerin fatty acid esters, polyglycerin fatty acid esters, sucrose fatty acid esters, sorbitan fatty acid esters, propylene glycol fatty acid esters, gum arabic, carrageenan, casein, gelatin, pectin, agar, B vitamins, nicotinamide, calcium pantothenate, amino acids, calcium salts, colorants, flavorings, preservatives, and other food ingredients.

[0021] As a specific manufacturing method, the allergic dermatitis preventive and therapeutic agent is spray-dried or freeze-dried together with powdered cellulose, and then made into a powder, granules, tablets, or solution, which can be easily incorporated into food and drink (instant food, etc.). The allergic dermatitis preventive and therapeutic agent can be dissolved in, for example, oils and fats, ethanol, glycerin, or a mixture thereof to make a liquid, which can be added to beverages or solid foods. If necessary, it can also be mixed with a binder such as gum arabic or dextrin to make a powder or granules, which can be added to beverages or solid foods.

[0022] When the preventive and therapeutic agent for allergic dermatitis of the present invention is applied to food and drink, the amount of the active ingredient added is preferably 1 to 20 wt % in total relative to the food and drink, since the main purpose is maintaining health and beauty.

[0023] The allergic dermatitis preventive and therapeutic agent of the present invention may be used as a material for pharmaceutical compositions (including pharmaceuticals and quasi-drugs). The allergic dermatitis preventive and therapeutic agent of the present invention can be appropriately mixed with raw materials for pharmaceutical preparations to produce the agent. Examples of raw materials for preparations that can be mixed with the allergic dermatitis preventive and therapeutic agent of the present invention include excipients (glucose, lactose, sucrose, sodium chloride, starch, calcium carbonate, kaolin, crystalline cellulose, cacao butter, hardened vegetable oil, kaolin, talc, etc.), binders (distilled water, physiological saline, ethanol water, simple syrup, glucose solution, starch solution, gelatin solution, carboxymethylcellulose, potassium phosphate, polyvinylpyrrolidone, etc.), disintegrants (sodium alginate, agar), and the like. , sodium bicarbonate, calcium carbonate, sodium lauryl sulfate, stearate monoglyceride, starch, lactose, powdered arabic, gelatin, ethanol, etc.), disintegration inhibitors (white sugar, stearin, cocoa butter, hydrogenated oils, etc.), absorption enhancers (quaternary ammonium bases, sodium lauryl sulfate, etc.), adsorbents (glycerin, starch, lactose, kaolin, bentonite, silicic acid, etc.), lubricants (purified talc, stearates, polyethylene glycol, etc.).

[0024] The method of administration of the allergic dermatitis preventive and therapeutic agent of the present invention can generally be oral administration in the form of tablets, pills, soft or hard capsules, fine granules, powders, granules, liquids, etc., but may also be parenteral administration. When administered as a parenteral agent, it may be administered in the form of a solution or with the addition of a dispersing agent, suspending agent, stabilizer, etc., by local tissue administration, intradermal, subcutaneous, intramuscular, or intravenous injection, etc. It may also be in the form of a suppository, etc.

[0025] The dosage may vary depending on the administration method, the condition, the age of the patient, etc., but typically, for adults, 0.5 to 5000 mg of the active ingredient can be administered per day, and for children, 0.5 to 3000 mg. The compounding ratio of the allergic dermatitis preventive and therapeutic agent can be appropriately changed depending on the dosage form, but it is usually about 0.3 to 15.0 wt% when administered orally or via mucosal absorption, and about 0.01 to 10 wt% when administered parenterally. Note that the dosage varies depending on various conditions, so in some cases a smaller dosage than the above is sufficient, and in other cases it is necessary to administer more than the range.

[0026] The agent for preventing and treating allergic dermatitis of the present invention can be expected to have an action as an agent for preventing and treating allergic dermatitis even when used as an external agent for skin (including cosmetics, medicines and quasi-drugs). Examples of the forms of external skin preparations that can be blended with the allergic dermatitis preventive and therapeutic agent of the present invention include milk lotions, soaps, facial cleansers, bath additives, creams, milk lotions, skin toners, colognes, shaving creams, shaving lotions, cosmetic oils, suntan and sunscreen lotions, face powders, foundations, perfumes, packs, nail creams, enamel, enamel removers, eyebrow pencils, blushers, eye creams, eye shadows, mascaras, eyeliners, lipsticks, lip balms, shampoos, rinses, hair dyes, dispersions, cleansers, etc. Examples of the forms of medicines or quasi-drugs that can be blended with the allergic dermatitis preventive and therapeutic agent of the present invention include ointments, creams, external liquids, etc.

[0027] In addition to the allergic dermatitis preventive and therapeutic agent of the present invention, the skin topical preparation in the above form may contain ingredients that are incorporated into skin topical preparations such as cosmetics and quasi-drugs, as long as the action of the allergic dermatitis preventive and therapeutic agent is not impaired, such as oils, higher alcohols, fatty acids, ultraviolet absorbers, powders, pigments, surfactants, polyhydric alcohols, sugars, polymers, physiologically active ingredients, solvents, antioxidants, fragrances, preservatives, etc. Examples are listed below, but the present invention is not limited to these examples.

[0028] (1) Example of oil Ester-based oil phase components: glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, butyl stearate, butyl myristate, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, diisopropyl adipate isopropyl, isoarachyl neopentanoate, tri(caprylic / capric)glyceryl, trimethylolpropane tri-2-ethylhexanoate, trimethylolpropane triisostearate, pentaerythritol tetra-2-ethylhexanoate, cetyl caprylate, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, cetyl ricinoleate, isostearyl laurate, isotridecyl myristate, isocetyl myristate, isostearate Tearyl, isocetyl palmitate, isostearyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, cetostearyl 2-ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicaprate, propylene glycol dicaprylate, propylene glycol dicaprylate, dicaprin Neopentyl glycol acid, neopentyl glycol dioctanoate, glyceryl tricaprylate, glyceryl triundecylate, glyceryl triisopalmitate, glyceryl triisostearate, octyldodecyl neopentanoate, isostearyl octanoate, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerin oleate, polyglycerin isostearate, dipropyl carbonate,Dialkyl carbonate (C12-18), triisocetyl citrate, triisoarachyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di-2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate propyl, dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, phytosteryl isostearate, phytosteryl oleate, isocetyl 12-stearoylhydroxystearate, stearyl 12-stearoylhydroxystearate, isostearyl 12-stearoylhydroxystearate, and the like. Hydrocarbon-based oil phase components: squalane, liquid paraffin, α-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybutene, microcrystalline wax, petrolatum, etc. Animal and vegetable oils and their hydrogenated oils, and naturally derived waxes: beef tallow, hydrogenated beef tallow, lard, hydrogenated lard, horse oil, hydrogenated horse oil, mink oil, orange roughy oil, fish oil, hydrogenated fish oil, egg yolk oil, and other animal oils and their hydrogenated oils, avocado oil, almond oil, olive oil, cocoa butter, kiwi seed oil, apricot kernel oil, kukui nut oil, sesame oil, wheat germ oil, rice germ oil, rice bran oil, safflower oil, shea butter, soybean oil, evening primrose oil, perilla oil, tea seed oil, camellia oil, corn oil, rapeseed oil, Examples of such oils include vegetable oils and their hydrogenated oils such as hydrogenated rapeseed oil, palm kernel oil, hydrogenated palm kernel oil, palm oil, hydrogenated palm oil, peanut oil, hydrogenated peanut oil, castor oil, hydrogenated castor oil, sunflower oil, grape seed oil, jojoba oil, hydrogenated jojoba oil, macadamia nut oil, medicamentoil, cottonseed oil, hydrogenated cottonseed oil, coconut oil, and hydrogenated coconut oil; and waxes such as beeswax, high acid value beeswax, lanolin, reduced lanolin, hydrogenated lanolin, liquid lanolin, carnauba wax, and montan wax. Silicone-based oil phase components: dimethylpolysiloxane, methylphenylpolysiloxane, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, methylhydrogenpolysiloxane, polyether-modified organopolysiloxane, dimethylsiloxane-methylcetyloxysiloxane copolymer, dimethylsiloxane-methylstearoxysiloxane copolymer, alkyl-modified organopolysiloxane, terminal-modified organopolysiloxane, amino-modified silicone oil, amino-modified organopolysiloxane, dimethiconol, silicone gel, acrylic silicone, trimethylsiloxysilicate, silicone RTV rubber, etc. Fluorine-based oil phase components: Perfluoropolyether, fluorine-modified organopolysiloxane, fluorinated pitch, fluorocarbon, fluoroalcohol, fluoroalkyl-polyoxyalkylene-co-modified organopolysiloxane, etc.

[0029] (2) Examples of higher alcohols Examples of the alcohol include lauryl alcohol, myristyl alcohol, cetyl alcohol, stearyl alcohol, isostearyl alcohol, oleyl alcohol, behenyl alcohol, 2-ethylhexanol, hexadecyl alcohol, and octyldodecanol.

[0030] (3) Examples of fatty acids Examples of the acid include caprylic acid, capric acid, undecylenic acid, lauric acid, myristic acid, palmitic acid, palmitoleic acid, stearic acid, isostearic acid, oleic acid, linoleic acid, linolenic acid, arachidic acid, arachidonic acid, behenic acid, erucic acid, and 2-ethylhexanoic acid.

[0031] (4) Examples of UV absorbers Para-aminobenzoic acid, amyl para-aminobenzoate, dihydroxypropyl ethyl para-aminobenzoate, glyceryl para-aminobenzoate, ethyl para-aminobenzoate, octyl para-aminobenzoate, octyldimethyl para-aminobenzoate, ethylene glycol salicylate, octyl salicylate, triethanolamine salicylate, phenyl salicylate, butylphenyl salicylate, benzyl salicylate, homomenthyl salicylate, benzyl cinnamate, octyl para-methoxycinnamate, 2-ethylhexyl para-methoxycinnamate, glyceryl di-para-methoxycinnamate mono-2-ethylhexanoate, isopropyl para-methoxycinnamate, diethanolamine salt of para-methoxyhydrocinnamate, mixture of diisopropyl and diisopropyl cinnamate, urocanic acid, ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and its salts, di Hydroxymethoxybenzophenone, dihydroxymethoxybenzophenone sodium disulfonate, dihydroxybenzophenone, dihydroxydimethoxybenzophenone, hydroxyoctoxybenzophenone, tetrahydroxybenzophenone, butylmethoxydibenzoylmethane, 2,4,6-trianilino-p-(carbo-2-ethylhexyl-1-oxy)-1,3,5-triazine, 2-(2-hydroxy-5-methylphenyl)benzotriazole, methyl-O-aminobenzoate, 2-ethylhexyl-2-cyano-3,3-diphenylacrylate, phenylbenzimidazole sulfate, 3-(4-methylbenzylidene)camphor, isopropyldibenzoylmethane, 4-(3,4-dimethoxyphenylmethylene)-2,5-dioxo-1-imidazolidinepropionate 2-ethylhexyl, and the like, as well as polymer derivatives and silane derivatives thereof.

[0032] (5) Examples of powders and pigments Dyes such as Red No. 104, Red No. 201, Yellow No. 4, Blue No. 1, Black No. 401, lake dyes such as Yellow No. 4 AL Lake, Yellow No. 203 BA Lake, nylon powder, silk powder, urethane powder, Teflon (registered trademark) powder, silicone powder, polymethyl methacrylate powder, cellulose powder, starch, silicone elastomer spherical powder, polyethylene powder and other polymers, yellow iron oxide, red iron oxide, black iron oxide, chromium oxide, carbon black, ultramarine, Prussian blue and other colored pigments, zinc oxide, titanium oxide, acid Examples of such pigments include white pigments such as cerium chloride, extender pigments such as talc, mica, sericite, kaolin, and plate-like barium sulfate, pearl pigments such as titanium mica, metal salts such as barium sulfate, calcium carbonate, magnesium carbonate, aluminum silicate, and magnesium silicate, inorganic powders such as silica and alumina, metal soaps such as aluminum stearate, magnesium stearate, zinc palmitate, zinc myristate, magnesium myristate, zinc laurate, and zinc undecylenate, bentonite, smectite, and boron nitride. There are no particular limitations on the shape (spherical, rod-like, needle-like, plate-like, irregular, flaky, spindle-like, etc.) and particle size of these powders. These powders may or may not have been previously surface-treated by a conventionally known surface treatment, such as a fluorine compound treatment, a silicone treatment, a silicone resin treatment, a pendant treatment, a silane coupling agent treatment, a titanium coupling agent treatment, an oil treatment, an N-acylated lysine treatment, a polyacrylic acid treatment, a metal soap treatment, an amino acid treatment, a lecithin treatment, an inorganic compound treatment, a plasma treatment, or a mechanochemical treatment.

[0033] (6) Examples of surfactants Anionic surfactants: fatty acid soaps, α-acylsulfonates, alkylsulfonates, alkylarylsulfonates, alkylnaphthalenesulfonates, alkyl sulfates, POE alkyl ether sulfates, alkylamide sulfates, alkyl phosphates, POE alkyl phosphates, alkylamide phosphates, alkyloylalkyl taurine salts, N-acylamino acid salts, POE alkyl ether carboxylates, alkylsulfosuccinates, sodium alkylsulfoacetates, acylated hydrolyzed collagen peptide salts, perfluoroalkyl phosphate esters, and the like. Cationic surfactants: alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyltrimethylammonium bromide, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, behenyltrimethylammonium bromide, benzalkonium chloride, behenic acid amidopropyldimethylhydroxypropylammonium chloride, stearate diethylaminoethylamide, stearate dimethylaminopropylamide, lanolin derivative quaternary ammonium salts, etc. Amphoteric surfactants: Examples of amphoteric surfactants include carboxybetaine type, amidobetaine type, sulfobetaine type, hydroxysulfobetaine type, amidosulfobetaine type, phosphobetaine type, aminocarboxylate type, imidazoline derivative type, and amidoamine type. Nonionic surfactants: Propylene glycol fatty acid esters, glycerin fatty acid esters, polyglycerin fatty acid esters, sorbitan fatty acid esters, POE sorbitan fatty acid esters, POE sorbit fatty acid esters, POE glycerin fatty acid esters, POE alkyl ethers, POE fatty acid esters, POE hydrogenated castor oil, POE castor oil, POE·POP copolymers, POE·POP alkyl ethers, polyether modified silicone lauric acid alkanolamide, alkylamine oxide, hydrogenated soybean phospholipids, etc. Natural surfactants: Examples include lecithin, saponin, sugar-based surfactants, etc.

[0034] (7) Examples of polyhydric alcohols and sugars Examples of suitable sugars include ethylene glycol, diethylene glycol, polyethylene glycol, propylene glycol, dipropylene glycol, polypropylene glycol, glycerin, diglycerin, polyglycerin, 3-methyl-1,3-butanediol, 1,3-butylene glycol, sorbitol, mannitol, raffinose, erythritol, glucose, sucrose, fructose, xylitol, lactose, maltose, maltitol, trehalose, alkylated trehalose, mixed isomerized sugar, sulfated trehalose, pullulan, etc. Chemically modified versions of these sugars may also be used.

[0035] (8) Examples of polymers Acrylic acid ester / methacrylic acid ester copolymer (Plussize, manufactured by GOO Chemical Co., Ltd.), vinyl acetate / crotonic acid copolymer (Resin 28-1310, manufactured by NSC Co., Ltd.), vinyl acetate / crotonic acid / vinyl neodecanoate copolymer (28-2930, manufactured by NSC Co., Ltd.), methyl vinyl ether maleic acid half ester (Gantrez ES, manufactured by ISP Co., Ltd.), t-butyl acrylate / ethyl acrylate / methacrylic acid copolymer (Rubymer, manufactured by BASF Co., Ltd.), vinyl pyrrolidone / vinyl acetate / vinyl propionate copolymer (Ruviscol VAP, manufactured by BASF Co., Ltd.), vinyl acetate Vinyl acetate / crotonic acid copolymer (Rubyset CA, BASF), vinyl acetate / crotonic acid / vinyl pyrrolidone copolymer (Rubyset CAP, BASF), vinyl pyrrolidone / acrylate copolymer (Rubyflex, BASF), acrylate / acrylamide copolymer (Ultrahold, BASF), vinyl acetate / butyl maleate / isobornyl acrylate copolymer (Advantage, ISP), carboxyvinyl polymer (Carbopol, BFGoodrich), acrylic acid / alkyl methacrylate copolymer (Pemulen, BF Examples of such polymers include anionic polymers such as acetic acid amphoteric product of dialkylaminoethyl methacrylate polymer (Yukaformer, manufactured by Mitsubishi Chemical Corporation) and amphoteric polymers such as octylacrylamide acrylate / hydroxypropyl acrylate / butylaminoethyl methacrylate copolymer (AMPHOMER, manufactured by NSC Corporation); cationic polymers such as quaternary product of vinylpyrrolidone / dimethylaminoethyl methacrylate (GAFQUAT, manufactured by ISP Corporation) and methylvinylimidazolium chloride / vinylpyrrolidone copolymer (Rubycoat, manufactured by BASF Corporation); and nonionic polymers such as polyvinylpyrrolidone (Ruviscol K, manufactured by BASF Corporation), vinylpyrrolidone / vinyl acetate copolymer (Ruviscol VA, manufactured by BASF Corporation), vinylpyrrolidone / dimethylaminoethyl methacrylate copolymer (copolymer 937, manufactured by ISP Corporation), and vinylcaprolactam / vinylpyrrolidone / dimethylaminoethyl methacrylate copolymer (copolymer VC713, manufactured by ISP Corporation).Also suitable for use are naturally occurring polymeric compounds such as cellulose or derivatives thereof, keratin and collagen or derivatives thereof, calcium alginate, pullulan, agar, gelatin, tamarind seed polysaccharides, xanthan gum, carrageenan, high methoxyl pectin, low methoxyl pectin, guar gum, gum arabic, crystalline cellulose, arabinogalactan, karaya gum, tragacanth gum, alginic acid, albumin, casein, curdlan, gellan gum, and dextran.

[0036] (9) Examples of physiologically active ingredients Examples of the physiologically active ingredient include substances that impart some physiological activity to the skin when applied to the skin. For example, they include whitening ingredients, immune activators, anti-aging agents, UV protection agents, slimming agents, tightening agents, antioxidants, hair growth agents, hair restorers, moisturizers, blood circulation promoters, antibacterial agents, bactericides, drying agents, cooling agents, warming agents, vitamins, amino acids, wound healing promoters, irritation relievers, analgesics, cell activators, enzyme ingredients, etc. Examples of suitable ingredients include angelica extract, avocado extract, amacha extract, althea extract, arnica extract, aloe extract, apricot extract, apricot kernel extract, ginkgo extract, fennel extract, turmeric extract, oolong tea extract, eijitsu extract, echinacea leaf extract, scutellaria extract, phellodendron bark extract, coptis japonica extract, barley extract, hypericum extract, white nettle extract, watercress extract, orange extract, Dried seawater, seaweed extract, hydrolyzed elastin, hydrolyzed wheat powder, hydrolyzed silk, chamomile extract, carrot extract, artemisia capillaris extract, licorice extract, kalkade extract, kakyoku extract, cinchona extract, cucumber extract, guanosine, gardenia extract, kumazasa extract, sophora flavescens extract, walnut extract, grapefruit extract, clematis extract, chlorella extract, mulberry extract, gentiana extract, black tea extract, yeast extract, burdock extract, coix seed extract, Fermented rice bran extract, rice germ oil, comfrey extract, collagen, lingonberry extract, Chinese ivy extract, Chinese rhizome extract, umbilical cord extract, salvia extract, soapwort extract, bamboo extract, hawthorn extract, Japanese pepper extract, shiitake mushroom extract, rehmannia extract, lithospermum extract, perilla extract, linden extract, meadowsweet extract, peony extract, calamus root extract, white birch extract, horsetail extract, ivy extract, hawthorn extract, se Elderberry extract, yarrow extract, peppermint extract, sage extract, mallow extract, cnidium extract, swertia britannica extract, soybean extract, tsinga extract, thyme extract, tea extract, clove extract, imperata cylindrica extract, tangerine peel extract, angelica extract, calendula extract, peach kernel extract, spruce extract, houttuynia cordata extract, tomato extract, natto extract, carrot extract, garlic extract, wild rose extract, hibiscus extract,Examples of such extracts include burdock root extract, parsley extract, honey, witch hazel extract, parietaria extract, burdock root extract, bisabolol, loquat extract, coltsfoot extract, butterbur bud extract, poria extract, butcher's broom extract, grape extract, propolis, loofah extract, safflower extract, peppermint extract, linden extract, peony extract, hop extract, pine extract, horse chestnut extract, skunk cabbage extract, soapberry extract, melissa extract, peach extract, cornflower extract, eucalyptus extract, saxifrage extract, coix seed extract, mugwort extract, lavender extract, apple extract, lettuce extract, lemon extract, astragalus extract, rose extract, rosemary extract, Roman chamomile extract, and royal jelly extract. In addition, it contains biopolymers such as deoxyribonucleic acid, mucopolysaccharides, sodium hyaluronate, sodium chondroitin sulfate, collagen, elastin, chitin, chitosan, and hydrolyzed eggshell membrane, moisturizing ingredients such as amino acids, hydrolyzed peptides, sodium lactate, urea, sodium pyrrolidone carboxylate, betaine, whey, and trimethylglycine, oily ingredients such as sphingolipids, ceramides, phytosphingosine, cholesterol, cholesterol derivatives, and phospholipids, ε-aminocaproic acid, glycyrrhizic acid, β-glycyrrhetinic acid, lysozyme chloride, and guaiazure. immune stimulants such as glycerin, hydrocortisone, etc.; vitamins such as vitamin A, vitamin B2, vitamin B6, vitamin C, vitamin D, vitamin E, calcium pantothenate, biotin, nicotinamide, vitamin C ester, etc.; active ingredients such as allantoin, diisopropylamine dichloroacetate, 4-aminomethylcyclohexanecarboxylic acid, etc.; antioxidants such as tocopherol, carotenoids, flavonoids, tannins, lignans, saponins, etc.; cell stimulants such as alpha-hydroxy acids, beta-hydroxy acids, etc.; blood circulation promoters such as gamma-oryzanol, vitamin E derivatives, etc. Wound healing agents such as retinol and retinol derivatives, skin whitening agents such as arbutin, kojic acid, placenta extract, sulfur, ellagic acid, linoleic acid, tranexamic acid, and glutathione, cepharanthine, licorice extract, capsicum tincture, hinokitiol, iodized garlic extract, pyridoxine hydrochloride, DL-α-tocopherol, DL-α-tocopherol acetate, nicotinic acid, nicotinic acid derivatives, calcium pantothenate, D-pantothenyl alcohol, acetyl pantothenyl ethyl ether, biotin, allantoin, isopropyl methylphenol, estradiol, All, ethinyl estradiol, capronium chloride, benzalkonium chloride, diphenhydramine hydrochloride, tacanal, camphor, salicylic acid, vanillylamide nonylate, vanillylamide nonanoate, piroctone olamine, glyceryl pentadecanoate, L-menthol, mononitroguaiacol, resorcinol, gamma-aminobutyric acid, benzethonium chloride, mexiletine hydrochloride, auxin, female hormones, cantharides tincture, cyclosporine, zinc pyrithione, hydrocortisone, minoxidil, polyoxyethylene sorbitan monostearate, peppermint oil,Examples include hair growth agents such as Sasanishiki extract.

[0037] (10) Examples of antioxidants Examples of antioxidants include sodium bisulfite, sodium sulfite, erythorbic acid, sodium erythorbate, dilauryl thiodipropionate, tocopherol, tolylbiguanide, nordihydroguaiaretic acid, parahydroxyanisole, butylhydroxyanisole, dibutylhydroxytoluene, ascorbyl stearate, ascorbyl palmitate, octyl gallate, propyl gallate, carotenoids, flavonoids, tannins, lignans, saponin, and plant extracts such as apple extract and clove extract that have antioxidant effects.

[0038] (11) Examples of solvents Examples of suitable solvents include purified water, ethanol, lower alcohols, ethers, LPG, fluorocarbons, N-methylpyrrolidone, fluoroalcohols, volatile linear silicones, and next-generation fluorocarbons.

[0039] The agent for preventing and treating allergic dermatitis of the present invention can be used as a raw material for food and drink compositions, pharmaceutical compositions, and external skin preparations. The pharmaceutical raw materials that can be blended with these can be the same as those used in the above-mentioned preventive and therapeutic agents for allergic dermatitis, and the manufacturing methods and administration methods can also be the same as those for the preventive and therapeutic agents for allergic dermatitis. EXAMPLES

[0040] The present invention will now be described with reference to examples. Example 1: Preparation of tomato seed extract, and isolation and identification of saponin components from tomato seeds 367.7 g of dried tomato seeds were extracted with methanol (70°C, 2 hours) to obtain a methanol extract. The obtained methanol extract was dispersed in water and sequentially partitioned and extracted with ethyl acetate and n-butanol to obtain 0.63 g of ethyl acetate soluble portion (ethyl acetate fraction), 2.41 g of n-butanol soluble portion (butanol fraction), and 5.05 g of water soluble portion (water fraction). Of these, the butanol fraction was used as the tomato seed extract of this example. The butanol fraction was then subjected to silica gel chromatography (chloroform:methanol = 9:1 → 7:3 → chloroform:methanol:water = 6:4:1 → methanol) to obtain Fr. 1 to 6. Fr. 3 (1.1 g) was subjected to reversed-phase ODS column chromatography (methanol concentration 20% → 50% → 80% → 100%) to obtain Fr.3-1 to Fr.3-4. The saponin compounds lycoperoside A (formula (1) above) (7.0 mg) and H (formula (2) above) (7.5 mg) were isolated from Fr.3-3 (mg) by reversed-phase HPLC (Inertsil ODS-SP, 70% methanol). Each compound was identified by comparing its 1H-NMR (600 MHz) and 13C-NMR (150 MHz) spectra with those in the literature.

[0041] Test Example 1. Effects on mouse pruritus model Tomato seed extract (500 mg / kg) or lycoperoside H (10 or 30 mg / kg) was orally administered to ddY mice, and 1 hour later, 50 μL of a 3% saline solution of compound 48 / 80 was subcutaneously administered to the back of the neck to induce scratching behavior due to itching caused by immediate allergy. After the first scratching behavior was induced, the mice were observed for 30 minutes and the number of scratches was counted. Diphenhydramine hydrochloride (30 mg / kg) was used as a positive control. The results are shown in Figure 1.

[0042] Results and Effects of the Examples in Test Example 1 As a result of the experiment, it was confirmed that the administration of tomato seed extract reduced the number of scratching episodes compared to the control group, as shown in Figure 1. It was also confirmed that the administration of lycoperoside H also reduced the number of scratching episodes in a concentration-dependent manner. This confirmed that lycoperoside H and tomato seed extract are effective as agents for suppressing itching caused by allergies.

[0043] Test Example 2. Evaluation by mouse ear PCA response Anti-DNP-IgE diluted 2000-fold with saline was administered intradermally to both ears of ddY mice to induce sensitization. Two days later, tomato seed extract (500 mg / kg) or lycoperoside H (10 or 30 mg / kg) was administered orally, and two hours later, 0.25 mg of DNP-BSA and 0.5% Evans blue in saline solution (0.25 mL) were administered via the tail vein to induce antigen-antibody reactions. 3 After 0 minutes, the mice were killed by cervical dislocation, both ears were excised, and the dye that had leaked into the ears was extracted and its absorbance at 620 nm was measured. The results are shown in Figure 2.

[0044] Results and Effects of the Examples in Test Example 2 As a result of the experiment, a significant suppression of pigment leakage was confirmed in the tomato seed extract (500 mg / kg) and lycoperoside H (10 mg / kg) administration groups (Figure 2). These results demonstrated that tomato seed extract and lycoperoside H have an inhibitory effect on immediate-type allergic reactions.

[0045] Test Example 3. Moisturizing effect in a 3D human epidermis model Tomato seed extract, lycoperoside H or lycoperoside A was added to the membrane side of human epidermal 3D cells, and RNA was extracted from the tissue collected 48 hours later. The mRNA expression of the filaggrin gene and ceramide synthase-3 (CerS3), a gene involved in the synthesis of ceramide, was analyzed by real-time RT-PCR. The results are shown in Figure 3 (filaggrin gene) and Figure 4 (CerS3). In addition, TEWL was measured over time from 24 to 72 hours of culture. The results are shown in Figure 5. In Figure 5, "TSE" is tomato seed extract, "LycoA" is lycoperoside A, and "LycoH" is lycoperoside H.

[0046] Results and Effects of the Examples in Test Example 3 As a result of the experiment, real-time RT-PCR showed that the expression of filaggrin was significantly increased by the addition of tomato seed extract (10 μg / mL), lycoperoside H (3 and 10 μM), and lycoperoside A (1 μM) (Figure 3). On the other hand, the expression of ceramide synthase-3 (CerS3), an enzyme related to ceramide synthesis, was significantly increased by the addition of tomato seed extract (1 and 3 μg / mL) and lycoperoside H (1, 3, and 10 μM) (Figure 4). Furthermore, the TEWL measurement showed that after 72 hours of culture, the TEWL of the tomato seed extract (10 μg / mL), lycoperoside H (10 μM), and lycoperoside A (10 μM)-added groups was significantly decreased compared to the control group (Figure 5). These results indicate that tomato seed extract and lycoperosides increase the moisture retention of the skin by increasing the expression of skin moisturizing factors.

[0047] Test Example 4. Evaluation of ceramide-related gene expression in a 3D human epidermis model Tomato seed extract, lycoperoside A or lycoperoside H was added to the membrane side of human epidermal 3D cells, and RNA was extracted from the tissue collected 48 hours later, and the mRNA expression of genes involved in ceramide synthesis (CerS, GCS, GBA, SMS, ASM) was analyzed by real-time RT-PCR. In addition, lipids in the stratum corneum separated from the tissue 7 days after culture were extracted, and stratum corneum ceramide was quantified by thin-layer chromatography (HPTLC). The results are shown in Figure 7 (GCS), Figure 8 (GBA), Figure 9 (SMS), Figure 10 (ASM), and Figure 11 (stratum corneum ceramide amount).

[0048] Results and Effects of the Examples in Test Example 4 As a result of the experiment, real-time RT-PCR showed that the gene expression of glucosylceramide synthase (GCS) was significantly increased by the addition of tomato seed extract (3 μg / mL), lycoperoside A (3 μM), and lycoperoside H (3 and 10 μM) (Fig. 7). In addition, the gene expression of β-glucocerebrosidase (GBA) was also significantly increased by the addition of tomato seed extract (3 and 10 μg / mL), lycoperoside A (3 and 10 μM), and lycoperoside H (10 μM) (Fig. 8). On the other hand, the gene expression of tomato seed bolting phingomyelin synthase (SMS) was significantly decreased by the addition of tomato seed extract (10 μg / mL) and lycoperoside H (1, 3, and 10 μM) (Fig. 9). No significant effect was observed for tomato seed extract, lycoperoside A, or lycoperoside H on the gene expression of acid sphingomyelinase (ASM) (Figure 10). This confirmed that they promote ceramide production in the left pathway in Figure 6. In addition, the results of quantification of stratum corneum ceramides by HPTLC confirmed that, among the ceramide species in the stratum corneum, ceramide NP was significantly increased by the addition of lycoperoside H (1 and 10 μM) (Figure 11). Although no significant effects were observed for other ceramide species, they showed a tendency to increase with the addition of tomato seed extract, lycoperoside A, and lycoperoside H.

[0049] Effects of the embodiment It was found that tomato seed extract and lycoperosides have an inhibitory effect on immediate-type allergic reactions and have an effect of relieving dry skin, thus confirming their effectiveness as agents for the prevention and treatment of allergic dermatitis.

[0050] Examples of formulations of the preventive and therapeutic agent for allergic dermatitis (tomato seed extract or lycoperosides) of the present invention are given below, but the present invention is not limited to these examples. Formulation example 1: Chewing gum Sugar 52.0wt% Gum base 20.0 Glucose 10.0 Syrup 16.0 Fragrance 0.5 Cherry Blossom Extract 0.5 Glucosylceramide 0.5 Allergic dermatitis prevention and treatment agent 0.5 100.0wt%

[0051] Mixture Example 2: Gummies Reduced starch syrup 38.0wt% Granulated sugar 20.0 Glucose 20.0 Gelatin 4.7 Wednesday 9.68 Kiwi juice 4.0 Kiwi flavor 0.6 Dye 0.02 Cherry Blossom Extract 1.0 Glucosylceramide 1.0 Allergic dermatitis prevention and treatment 1.0 100.0wt%

[0052] Mixture example 3: Candy Sugar 50.0wt% Syrup 33.0 water 14.2 organic acid 2.0 Fragrance 0.2 Cherry Blossom Extract 0.1 Glucosylceramide 0.1 Allergic dermatitis prevention and treatment agent 0.4 100.0wt%

[0053] Mixture example 4: Yogurt (hard / soft) Milk 41.5wt% Skim milk powder 5.8 Sugar 8.0 Agar 0.15 Gelatin 0.1 Lactic acid bacteria 0.005 Cherry Blossom Extract 0.1 Glucosylceramide 0.1 Allergic dermatitis prevention and treatment agent 0.4 fragrance trace amount water residue 100.0wt%

[0054] Formulation example 5: Soft drink Fructose glucose liquid sugar 30.0wt% Emulsifier 0.5 Strawberry Seed Extract 0.05 Glucosylceramide 0.05 Allergic dermatitis prevention and treatment agent 0.05 Fragrance (appropriate amount) Purified water remainder 100.0wt%

[0055] Formulation example 6: Soft capsule Rice germ oil 86.0wt% Cherry Blossom Extract 0.5 Glucosylceramide 0.5 Emulsifier 12.0 Allergic dermatitis prevention and treatment 1.0 100.0wt%

[0056] Formulation Example 7: Tablets Lactose 53.0wt% Crystalline cellulose 30.0 Starch decomposition product 10.0 Cherry Blossom Extract 0.5 Glucosylceramide 0.5 Glycerin fatty acid ester 5.0 Allergic dermatitis prevention and treatment 1.0 100.0wt%

[0057] Formulation Example 8: Granules for oral administration (pharmaceutical) Allergic dermatitis prevention and treatment agent 1.0wt% Strawberry Seed Extract 0.5 Glucosylceramide 0.5 Lactose 30.0 Cornstarch 60.0 Microcrystalline cellulose 7.0 Polyvinylpyrrolidone 1.0 100.0wt%

[0058] Formulation example 9: Tablet confectionery Sugar 75.4wt% Glucose 19.0 Sucrose fatty acid ester 0.2 Strawberry Seed Extract 0.5 Glucosylceramide 0.5 Allergic dermatitis prevention and treatment agent 0.5 Purified water 3.9 100.0wt%

[0059] Example 10: Cat food Corn 33.0wt% Wheat flour 35.0 Meat meal 15.0 Beef tallow 8.9 Salt 1.0 Bonito extract 4.0 Strawberry Seed Extract 0.5 Glucosylceramide 0.5 Allergic dermatitis prevention and treatment 1.0 Taurine 0.1 Vitamins 0.5 Minerals 0.5 100.0wt%

[0060] Example 11: Dog food Corn 30.0wt% Meat (chicken) 15.0 Defatted soybeans 10.0 Wheat flour 24.0 Bran 5.0 Cherry Blossom Extract 0.5 Glucosylceramide 0.5 Allergic dermatitis prevention and treatment 5.0 Animal fats and oils 8.9 Oligosaccharides 0.1 Vitamin 0.5 Minerals 0.5 100.0wt%

[0061] Formulation example 12: Cosmetic cream Squalane 20.0wt% Beeswax 5.0 Refined Jojoba Oil 5.0 Glycerin 5.0 Glycerin Monostearate 2.0 Polyoxyethylene (20) Sorbitan- Monostearate 2.0 Allergic dermatitis prevention and treatment 2.0 Preservatives (appropriate amount) Fragrance (appropriate amount) Purified water remainder 100.0wt%

[0062] Formulation example 13: Lotion Ethanol 5.0wt% Glycerin 2.0 1,3-Butylene glycol 2.0 Polyethylene oleyl ether 0.5 Sodium citrate 0.1 Citric acid 0.1 Allergic dermatitis prevention and treatment agent 0.1 Purified water remainder 100.0wt%

[0063] Formulation example 14: Body gel Macadamia nut oil 2.0wt% Octyldodecyl myristate 10.0 Methylphenylpolysiloxane 5.0 Behenyl Alcohol 3.0 Stearic Acid 3.0 Batyl alcohol 1.0 Glyceryl Monostearate 1.0 Polyoxyethylene sorbitol tetraoleate 2.0 Hydrogenated soy phospholipids 1.0 Ceramide 0.1 Retinol Palmitate 0.1 Preservatives (appropriate amount) Centella asiatica extract 1.0 Allergic dermatitis prevention and treatment 1.0 1,3-Butylene glycol 5.0 Purified water remainder 100.0wt%

[0064] Formulation example 15: Emulsion Squalane 4.0wt% Vaseline 2.5 Cetanol 2.0 Glycerin 2.0 Lipophilic glyceryl monostearate 1.0 Stearic acid 1.0 L-Arginine 1.0 Allergic dermatitis prevention and treatment agent 0.5 Potassium hydroxide 0.1 fragrance trace amount Purified water remainder 100.0wt%

[0065] Formulation example 16: Bath additive (liquid) Propylene glycol 50.0wt% Ethanol 20.0 Sodium sulfate 5.0 Allergic dermatitis prevention and treatment agent 0.5 Lanolin 0.5 Avocado oil 0.5 Dye 1.5 Fragrance 22.0 100.0wt% [Industrial Applicability]

[0066] As described above, the present invention can provide a novel agent for preventing and treating allergic dermatitis.

Claims

1. A moisturizing agent comprising at least one active ingredient selected from lycoperoside A, lycoperoside H and tomato seed extract.

2. A filaggrin gene expression promoter comprising at least one active ingredient selected from lycoperoside A, lycoperoside H and tomato seed extract.

3. A ceramide synthase-3 gene expression promoter containing tomato seed extract as an active ingredient.

4. A GCS gene expression promoter comprising at least one of tomato seed extract, lycoperoside A, and lycoperoside H as an active ingredient.

5. A GBA gene expression promoter comprising at least one of tomato seed extract, lycoperoside A, and lycoperoside H as an active ingredient.

6. An SMS gene expression inhibitor containing at least one of tomato seed extract and lycoperoside H as an active ingredient.

7. A ceramide NP production promoter whose active ingredient is lycoperoside H.

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