Method for analyzing substances such as nucleic acid drugs

By preparing a mobile phase with a basic ion pair reagent in a non-aqueous solvent and using inert gas bubbling, the method maintains high sensitivity in liquid chromatography-mass spectrometry for analyzing ionic substances like nucleic acid drugs, addressing the issue of mobile phase deterioration.

JP2025010360AActive Publication Date: 2025-01-20LSI MEDIENCE CORPORATION
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Patent Information

Application Number
JP2024192263
Authority / Receiving Office
JP · JP
Patent Type
Applications
Current Assignee / Owner
Priority Date
2020-07-17
Filing Date
2024-10-31
Publication Date
2025-01-20
Estimated Expiration
2041-02-24

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Abstract

To provide means that enables continuous analysis of ionic substances, such as nucleic acid drugs, to be conducted over a long period of time while maintaining high sensitivity in liquid chromatography-mass spectrometry.SOLUTION: An analysis method includes the step for subjecting a sample containing an ionic analyte to liquid chromatography using a mobile phase containing a basic ion-pair reagent and further subjecting the analyte to mass spectrometry. A first mobile phase containing a basic ion-pair reagent in a nonaqueous solvent and a second mobile phase of an aqueous solvent containing no basic ion-pair reagent are prepared in separate containers, and are mixed for use when subjected to the liquid chromatography.SELECTED DRAWING: None
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Citation Information

Patent Citations

  • Methods for analyzing oligonucleotides

    JP2012500394A