Skin beautification method, multiple component skin permeation method, and multiple component skin permeation system

Abstract

To achieve a sufficient rejuvenation effect to enhance the user's level of satisfaction.SOLUTION: Provided is a skin beautification method (excluding medical treatment) including: a step for applying a first composition containing one or more substances selected from a sirtuin gene activator, a growth factor agent, a proteoglycan, and a cannabinoid; and a step for applying a second composition containing one or more substances, other than the first composition, selected from the sirtuin gene activator, the growth factor agent, the proteoglycan, and the cannabinoid.SELECTED DRAWING: Figure 3

Description

[Technical field]

[0001] The present invention relates to a skin cosmetic method, a multiple component skin permeation method, and a multiple component skin permeation system for performing skin cosmetic treatment. [Background technology]

[0002] A skin beauty composition containing an acetic acid extract of onion bulbs and baking soda has been proposed as a skin beauty composition that improves wrinkles and has an excellent feel when used, and a skin beauty method (excluding medical procedures) of applying the composition has been proposed (see Patent Document 1). In this skin beauty method, the onion bulb acetic acid extract alone is effective in improving wrinkles, moisturizing, and beautifying the skin (improving skin texture), but it has been found that by adding baking soda, it is possible to achieve even better skin beauty effects (especially improving wrinkles) in a shorter period of time. In the beauty industry, there is a demand for further effects, such as a youthful appearance effect, i.e., a lifting effect that improves facial sagging and wrinkles, in addition to wrinkle improvement, moisturizing effect, and skin beautifying effect. However, the above-mentioned cosmetic methods do not provide a lifting effect. [Prior art documents] [Patent documents]

[0003] [Patent Document 1] Patent No. 6973740 Summary of the Invention [Problem to be solved by the invention]

[0004] In view of the above-mentioned problems, an object of the present invention is to provide a sufficient effect of making a person look younger and to improve the satisfaction of the user. [Means for solving the problem]

[0005] This invention is characterized as a skin beauty method (excluding medical procedures) comprising the steps of applying a first composition containing one or more selected from a sirtuin gene activator, a growth factor agent, a proteoglycan, and a cannabinoid, and applying a second composition containing one or more agents other than the first composition selected from the sirtuin gene activator, the growth factor agent, the proteoglycan, and the cannabinoid. Effect of the Invention

[0006] According to the present invention, it is possible to provide a skin beauty method, a multiple component skin permeation method, and a multiple component skin permeation system that can provide a sufficient youthful appearance effect. [Brief description of the drawings]

[0007] [Figure 1] System diagram of a multi-component skin penetration system. [Diagram 2] FIG. 1 is a block diagram showing the configuration of a multiple component skin penetration system. [Diagram 3] 1 is a flow chart illustrating the operation of the multiple component skin penetration system. [Figure 4] An illustration of the before and after effects of the multi-ingredient skin penetration system. [Diagram 5] FIG. 1 is a diagram showing the composition of a kit. [Figure 6] An explanatory diagram of before and after using the composition kit. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0008] In this invention, a first composition containing a first active ingredient and a second composition containing a second active ingredient are applied to the skin separately or in combination. The first active ingredient and the second active ingredient are a growth factor agent such as a human stem cell culture agent, and the other is a sirtuin gene activator such as NMN. As a method of application, the first penetrating agent consisting of the first active ingredient and the second penetrating agent consisting of the second active ingredient can be adjusted with an adjuster such as purified water, or together, to form a spray liquid (application liquid), which can then be applied. As a method of application, application can be performed using a penetrating device that promotes penetration into the skin, application with fingers, or application with a pack. The penetration device may be any suitable device, such as a spray device that sprays, an iontophoresis device that passes a weak negative current toward the skin to allow negatively ionized beauty ingredients to penetrate the skin, an electroporation device that applies an electric pulse to the skin to temporarily open minute holes in the cell membrane and allow the beauty ingredients to penetrate to the dermis, a roller-type penetration promoter with a plurality of minute needles provided on the circumferential surface of a cylindrical roller, etc. The skin referred to here refers to all the skin of the body, such as the skin of the face, neck, décolleté, limbs, chest, abdomen, back, pubic area, and head. Hereinafter, an embodiment of the present invention will be described with reference to the drawings.

[0009] FIG. 1 is a diagram showing the system configuration of a multiple component skin penetration system 1. As shown in FIG. The multiple component skin penetration system 1 is comprised of a first penetrating agent 2, a second penetrating agent 3, a conditioning agent 4, and a spray device 5.

[0010] The first penetrating agent 2 is an agent containing a first active ingredient to be penetrated into the skin, and is composed of a liquid or powder. The second penetrating agent 3 is an agent containing a second active ingredient to be penetrated into the skin, and is composed of a liquid or powder.

[0011] In this embodiment, one of the first penetrating agent 2 (first active ingredient) and the second penetrating agent 3 (second active ingredient) is a sirtuin gene activator such as NMN (NMN agent), and the other is a growth factor agent such as a human stem cell culture agent.

[0012] The sirtuin gene activator can be an NAD+ precursor, an NAD+ analogue, NAD (NAD+), niacinamide or a combination thereof, and can be in the form of a powder or liquid. The NAD+ precursor can be nicotinamide mononucleotide (hereinafter referred to as NMN). The NAD+ analogue can be deazaflavin or 5-deazaflavin. The 5-deazaflavin can be 5-deazaflavin (TND1128) or 5-deazaflavin (F420) or both. The sirtuin gene activator can be in the form of a powder or liquid, and in the case of a powder, it can be powdered by microgranulation or freeze-drying.

[0013] NMN is a powder or liquid form of nicotinamide mononucleotide (NMN) that is found in breast milk, edamame, broccoli, avocado, tomato, and other organisms. When NMN is in liquid form, it is extracted from the organisms listed above. When it is in powder form, it is formed by granulation, such as microgranulation.

[0014] The growth factor agent may be a growth factor, an exosome, a stem cell culture supernatant agent, or a combination thereof, and may be in the form of a powder or liquid. The growth factor may be EGF (epidermal growth factor), FGF (fibroblast growth factor), PDGF (platelet-derived growth factor), IGF-1 (insulin-like growth factor), HGF (hepatocyte growth factor), KGF (epidermal growth factor), TGF-β (transforming growth factor), or a combination thereof. The FGF may be any one of the FGF family, and may be any one or more of FGF1 to FGF23, preferably any one or more of FGF1 to FGF14, more preferably any one or more of FGF1 to FGF10, and even more preferably FGF1 or FGF2. The growth factor agent may be in the form of a liquid or powder. In the case of a powder, the liquid growth factor agent may be freeze-dried by a freeze-drying method and powdered to obtain the desired FGF.

[0015] The growth factor agent is derived from human stem cell culture supernatant, which can be obtained by culturing human stem cells of an appropriate origin, such as umbilical cord origin, umbilical cord blood origin, fat origin, or dental pulp origin, and can be in the form of a liquid or powder.

[0016] In addition, in the culture of human stem cells, it is preferable to subculture the cells for a certain number of generations, and preferably for up to 5 generations. It is also preferable to subculture the cells while the growth curve during culture is linear. This can prevent contamination with senescent cells and prevent contamination with growth factors from senescent cells.

[0017] The human stem cell culture agent is a supernatant obtained when stem cells collected from a human are cultured in a culture medium, or a powder obtained by freeze-drying the supernatant using a freeze-drying method, and can be used as a human stem cell culture supernatant agent. The human stem cell culture supernatant agent can be a human umbilical cord stem cell culture supernatant obtained from a human umbilical cord, a human umbilical cord blood stem cell culture supernatant obtained from a human umbilical cord blood, a human adipose stem cell culture supernatant obtained from a human fat, a human bone marrow stem cell culture supernatant obtained from a human bone marrow fluid, a supernatant obtained by culturing other human cells, or a supernatant powder obtained by powdering these supernatants using a freeze-drying method.

[0018] The first penetrating agent 2 and the second penetrating agent 3 are preferably each 100% sirtuin gene activator or 100% growth factor agent, but may each contain other components. Note that all % notations in this specification are expressed in mass percent.

[0019] The volume of the sirtuin gene activator may be 10 ml or more, preferably 50 ml or more, more preferably 100 ml or more, even more preferably 150 ml or more, and even more preferably 200 ml or more. The volume of the sirtuin gene activator may be 1000 ml or less, preferably 800 ml or less, more preferably 600 ml or less, and even more preferably 500 ml or less.

[0020] The amount of growth factor agent may be 0.1 mg or more, preferably 0.5 mg or more, and more preferably 1 mg or more, and may be 20 mg or less, preferably 15 mg or less, and more preferably 10 mg or less.

[0021] The adjusting agent 4 can be purified water or physiological saline. The purified water can be ordinary purified water or sterilized purified water obtained by sterilizing purified water. The physiological saline can be any appropriate one, such as injectable saline obtained by sterilizing purified water through filtration or distillation and removing pyrogens.

[0022] The amount of the regulator 4 may be 0.5 ml or more, preferably 1 ml or more, and more preferably 2 ml or more. The amount of the regulator 4 is preferably 20 ml or less, more preferably 15 ml or less, even more preferably 10 ml or less, and suitably 5 ml or less.

[0023] The ratio of the growth factor agent, the sirtuin gene activator, and the regulator 4 can be within the range of each of the amounts described above. Therefore, if the product is applied mainly to the face or scalp, the above amounts are sufficient, but if the product is applied to the whole body, the amounts can be increased within the above ranges.

[0024] The first penetrant 2 is adjusted with the adjuster 4 and used as the first spray liquid (first application liquid). Similarly, the second penetrant 3 is adjusted with the adjuster 4 and used as the second spray liquid (second application liquid). The first penetrant 2 and the second penetrant 3 can also be mixed and adjusted with the adjuster 4 to be used as a single integrated spray liquid (integrated application liquid).

[0025] The spray liquid (first spray liquid, second spray liquid, or integrated spray liquid) prepared in this manner can have a viscosity of 200 mPa·s or less, preferably 100 mPa·s or less, more preferably 50 mPa·s or less, more preferably 10 mPa·s or less, even more preferably 5 Pa·s or less, more preferably 3 mPa·s or less, even more preferably 2 mPa·s or less, and preferably 1 mPa·s or less. This makes it possible to prevent clogging inside the spray nozzle 12a of the spray device 5 described below when spraying.

[0026] The spray device 5 is a device that sprays pressurized liquid onto a target (human skin in this embodiment) through a nozzle, and will be described in detail below.

[0027] The spray device 5 has a main body 30 for delivering air, a conduit 20 having one end connected to the main body 30, and a handpiece 10 connected to the other end of the conduit 20 and operated by the practitioner.

[0028] The handpiece 10 is a longitudinal unit that can be held by the practitioner, and is provided with a spray cover 11 at the tip and the other end of the conduit 20 connected to the rear end. A liquid input section 15 is provided on the tip side of the upper surface of the handpiece 10 from the center. The liquid input section 15 is provided with a liquid input hole 15b on the cover of the upper surface, and a liquid storage section 15a is provided inside the handpiece 10. The liquid storage section 15a is a sterile room, and the liquid to be sprayed is input into the liquid storage section 15a from the liquid input hole 15b using a syringe or the like. A setting section 19 is provided on the rear end side of the upper surface of the handpiece 10 from the center. The setting section 19 has a display section 19a (see FIG. 2) that displays the current spray setting, a switching button 19b (see FIG. 2) that switches the spray setting of the spray liquid, an operation button 19c (see FIG. 2) that switches between starting and stopping the spray of the spray liquid, and a control section 19d that executes various controls. A dial-type spray amount adjustment unit 18 that adjusts the amount of spray liquid per spray is provided on the right side of handpiece 10 (front of Figure 1), and a manual spray button 16 that is pressed down during manual spraying to set the spray amount is provided on the left side of handpiece 10 (rear of Figure 1).

[0029] The conduit 20 has a pipe 20a (see FIG. 2) for supplying gas (air) to the handpiece 10 and an electric wire 20b (see FIG. 2) for supplying power to the handpiece 10 inside. The conduit 20 can be bent freely, so that the practitioner can freely move the handpiece 10 for treatment while leaving the main body 30 in place.

[0030] The main body 30 has an operation start button 30a on the front for switching between starting and stopping operation, and a power cord (not shown) on the back for connecting to a household power source, and one end of the conduit 20 is connected to the side.

[0031] FIG. 2 is a block diagram showing the configuration of the multiple component skin penetration system 1. Inside the handpiece 10, a spray tip 12 is provided on the tip side, and a transparent, approximately cylindrical spray cover 11 is attached to the outer periphery of this spray tip 12. This spray cover 11 is cylindrical, with the tip cut perpendicular to the axis of the cylinder, and two gas release spaces 11a are provided at the tip, one on the left and one on the right, facing each other. That is, the tip is mostly a circular ring-shaped tip on a plane, and at least a part of it is a recess that becomes the gas release space 11a. In the illustrated example, this gas release space 11a is formed by a recessed cutout groove that is partially switched at the end that is the open part of the spray cover 11.

[0032] A spray nozzle 12a for spraying the liquid to be sprayed is provided at the center of the spray tip 12, which is the tip of the handpiece 10, and a gas discharge section 14 is provided at the back of the spray nozzle 12a. A spray gas chamber 14b is provided inside the gas discharge section 14, and this spray gas chamber 14b is connected to the spray nozzle 12a. The air port 14a at the tip of the spray gas chamber 14b and the spray nozzle 12a are provided opposite each other. A spray liquid chamber 13 is provided on the outer periphery between the air port 14a and the spray nozzle 12a, and this spray liquid chamber 13 is connected to the spray nozzle 12a. The spray liquid chamber 13 is a sterile room and is connected to a liquid storage section 15a in which the liquid to be sprayed is stored. In addition, the discharge speed of the liquid to be sprayed and the gas discharged from the gas discharge section 14 through the spray nozzle 12a can be 100 km / h or more, preferably 500 km / h or more, and preferably 1,000 km / h or more. In addition, the upper limit of this discharge speed can be 5,000 km / h or less, preferably 3,000 km / h or less, and preferably 2,000 km / h or less. By discharging the compressed gas at high speed in a short period of time in this way, the liquid to be sprayed can be nano-sized and discharged. By discharging (applying) the liquid to be sprayed in this nano-sized form, the liquid to be sprayed can be penetrated deeper and more into the skin.

[0033] The outer periphery and the spray tip side of the spray tip 12 having the spray nozzle 12a are covered by the spray cover 11. Therefore, the spray liquid and gas sprayed from the spray nozzle 12a are confined within the spray cover 11 and are sprayed (ejected) vigorously onto the skin to be sprayed, and excess gas (including the spray liquid) is discharged to the outside through the gas release space 11a.

[0034] A solenoid 17 is provided in the handpiece 10 at the rear of the gas discharge unit 14. This solenoid 17 is provided midway through a pipe 20a that supplies gas to the spray gas chamber 14b of the gas discharge unit 14, and switches between passing and blocking the gas through the pipe 20a. This solenoid 17 and the control unit 19d that controls it function as a spray control unit.

[0035] In addition, inside the handpiece 10, a control unit 19d (see FIG. 1) is provided. In response to the operation of the switching button 19b, the control unit 19d switches the spray setting of the spray liquid to one of the following: manual spraying, which executes spraying once when the manual spray button 16 is pressed; first intermittent spraying, which performs instantaneous short-time spraying (e.g., 0.1 seconds, etc.) continuously at a first time interval (e.g., 1.5 seconds); second intermittent spraying, which performs short-time spraying (e.g., 0.1 seconds, etc.) continuously at a second time interval (e.g., 1.0 seconds interval) shorter than the first intermittent spray; and third intermittent spraying, which performs short-time spraying (e.g., 0.1 seconds, etc.) continuously at a third time interval (e.g., 0.5 seconds interval) even shorter than the second intermittent spray. The instantaneous short-time spray can be spraying for 1 second or less, preferably spraying for 0.5 seconds or less, more preferably spraying for 0.3 seconds or less, even more preferably spraying for 0.2 seconds or less, and preferably spraying for 0.1 seconds or less. The lower limit of the instantaneous short-term spray can be 0.001 seconds or more, preferably 0.01 seconds or more, and more preferably 0.05 seconds or more.

[0036] In addition, when the manual spray button 16 is pressed when the spray setting is manual spray (or when the time comes to spray with the first intermittent spray, second intermittent spray, or third intermittent spray), the control unit 19d executes spray control by turning on the solenoid 17 for a momentary predetermined time (for example, 0.1 seconds) during which spraying is performed, opening the pipe 20a to allow gas to pass, and then turning off the solenoid 17 to close the pipe 20a to prevent gas from passing. By controlling spraying in this way, the moment the solenoid 17 is turned off, negative pressure is generated in the spray liquid chamber 13, and the liquid to be sprayed in the liquid storage unit 15a is supplied to the spray liquid chamber 13, and the liquid to be sprayed together with the gas is sprayed from the spray nozzle 12a due to the instantaneous supply of gas when the solenoid 17 is next turned on.

[0037] The control unit 19d also executes an operation of displaying the current spray setting, and an error code, etc., in the event of an error, on the display unit 19a. Moreover, the control unit 19d executes these operations after the operation button 19c is turned on, and stops each operation when the operation button 19c is turned off. When the solenoid 17 is in the OFF state, the gas supplied from the pipe 20a flows into an exhaust path not shown.

[0038] The manual spray button 16, the solenoid 17, the display unit 19a, the switching button 19b, the operation button 19c, and the control unit 19d are supplied with power from the main body 30 via an electric wire 20b.

[0039] A spray amount adjustment unit 18 is provided downstream of the solenoid 17 in the handpiece 10. This spray amount adjustment unit 18 acts on the pipe 20a to adjust the amount of gas supplied when the solenoid 17 is turned on. This makes it possible to adjust the amount of gas and liquid to be sprayed that is ejected in one spray. The spray amount adjustment unit 18 may be configured to adjust the amount of spray by changing the opening size of the spray nozzle 12a. In this case, the spray range and spray intensity can also be adjusted.

[0040] The main body 30 is provided with a control unit 31, a power supply unit 32, and a gas supply unit 33. The control unit 31 controls the supply of gas by the gas supply unit 33. Specifically, when the operation start button 30a (see FIG. 1) is pressed to turn the power ON, the gas supply unit 33 is driven to start the supply of gas and starts the supply of power from the electric wire 20b to the unit inside the handpiece 10, and when the operation start button 30a (see FIG. 1) is pressed again to turn the power OFF, the supply of gas and power is stopped.

[0041] A power supply unit 32 supplies power to each unit. While the power is ON, the gas supply unit 33 drives an internal pump to continue to send gas through the pipe 20a to the nebulizing gas chamber 14b of the handpiece 10. In this embodiment, ambient air is sent as the gas, but it is possible to supply an appropriate gas such as oxygen, hydrogen, or an appropriate mixed gas by attaching a gas cylinder (not shown).

[0042] FIG. 3 is a flow chart illustrating the operation of the multiple component skin penetration system 1. When the operation start button 30a (see FIG. 1) is pressed to turn on the power, the control unit 19d receives the first liquid to be sprayed from the liquid inlet hole 15b (step S1). The received first liquid to be sprayed is supplied from the liquid storage unit 15a to the liquid chamber 13.

[0043] The control unit 19d accepts switching of the spray setting by the switching button 19b (step S2).

[0044] When the operating button 19c is pressed, the control unit 19d performs a first spray liquid spraying step of performing spraying according to the spray setting (step S3). That is, if the spray setting is manual spraying, spraying is performed at the timing when the manual spray button 16 is pressed, if the spray setting is the first intermittent spray, intermittent spraying at the first time interval is automatically and continuously performed, if the spray setting is the second intermittent spray, intermittent spraying at the second time interval is automatically and continuously performed, and if the spray setting is the third intermittent spray, intermittent spraying at the third time interval is automatically and continuously performed.

[0045] When the spray setting is changed (step S4: YES), the control unit 19d accepts the change in the spray setting (step S2), and executes spraying with the changed spray setting (step S3).

[0046] When spraying this spray liquid (first spray liquid, second spray liquid, etc.), the tip of spray cover 11 is brought into contact with the skin to perform spraying. When the circular ring-shaped tip of spray cover 11 is brought into full contact with the skin to perform spraying, excess gas is released from gas release space 11a at the tip together with the spray liquid. For this reason, most of the fine mist of spray liquid penetrates into the skin due to the force of release, and excess gas is released from gas release space 11a to prevent the skin from being pressed too hard by the gas pressure.

[0047] In other words, if the gas release space 11a does not exist and the entire circumference of the tip of the spray cover 11 is in contact with the skin when spraying, the released gas and spray liquid will press against the skin, and the amount of gas in the spray cover 11 will cause the skin to remain depressed. This will cause extra pressure on the skin, which may actually slow down the blood flow and hinder the penetration of the spray liquid. In contrast, by releasing the extra gas from the gas release space 11a, the skin will not be continuously pressured, and the skin will return to its original normal state immediately after the gas and spray liquid are sprayed onto the skin. Therefore, the permeated spray liquid can dissolve into the blood flow and lymph flow without stress, and a light and moderate stimulation is given to the skin, promoting the blood flow and lymph flow, allowing the permeated spray liquid to penetrate deeper.

[0048] If the process is not terminated even after the spraying of the first spray liquid is completed (step S5: NO), a second spray liquid spraying process is performed to receive the second spray liquid (step S6). The received second spray liquid is supplied from the liquid storage section 15a to the spray liquid chamber 13. After this, the process from step S2 onwards is repeated, and the second spray liquid is sprayed.

[0049] The above configuration and operation allow the active ingredient to be sprayed in a fine mist. Since only a small amount of spray liquid is used in one spray (instantaneous spray for 0.1 seconds or the like), even valuable and expensive active ingredients can be sprayed in small amounts many times, so that the active ingredient can be sprayed on the entire face by spraying one part at a time in sequence, and it is also possible to spray the neck and décolleté. The active ingredient can also be sprayed on the head, and the entire head can be sprayed, or multiple sprays can be applied to parts of the head where intensive penetration is desired.

[0050] When a growth factor agent such as human stem cell culture fluid is used as the penetrating agent (first penetrating agent or second penetrating agent), spraying it onto the skin of the face, neck, décolleté, etc. can increase firmness, provide a lifting effect, open the eyes, reduce wrinkles, slim the contours, make the skin look fresh and rejuvenated, etc. In particular, when a human umbilical cord stem cell culture supernatant or a growth factor agent derived from a human umbilical cord stem cell culture supernatant is used, these effects are more pronounced.

[0051] In addition, when human stem cell culture fluid is sprayed on a wounded or burned area of ​​the skin, the wound or burn will heal faster than usual, and will leave a clean wound without any scars after healing. In particular, when human umbilical cord stem cell culture supernatant or a growth factor agent derived from human umbilical cord stem cell culture supernatant is used, these effects are more pronounced.

[0052] Furthermore, when a growth factor agent such as human stem cell culture fluid is used as the penetrating agent (first penetrating agent or second penetrating agent), spraying it onto the head can produce hair growth effects and a youthful appearance, such as hair growth, hair volume increase, hair line advancement, hair firmness, hair strength, thickening, and gray hair turning back to black. In particular, when a human umbilical cord stem cell culture supernatant or a growth factor agent derived from a human umbilical cord stem cell culture supernatant is used, these effects are more pronounced.

[0053] Furthermore, when a sirtuin gene activator such as NMN is used as the penetrating agent (first penetrating agent or second penetrating agent), spraying it on the skin of the face, neck, décolleté, etc. can increase firmness, provide a lifting effect, open the eyes, reduce wrinkles, slim the contours, and make the skin look fresh and rejuvenated.

[0054] In addition, when a sirtuin gene activator such as NMN is sprayed on an injured or burned area of ​​the skin, the wound or burn will heal faster than usual and will leave a clean wound with no scars even after healing.

[0055] In addition, when a sirtuin gene activator such as NMN is used as the penetrating agent (first penetrating agent or second penetrating agent), spraying it on the head can have the following effects: hair growth, increased hair volume, advancement of the hairline, increased hair firmness, stronger hair strength, thicker hair, and turning gray hair back to black.

[0056] Furthermore, when a growth factor agent such as human stem cell culture medium is used as one of the first and second penetrating agents, and a sirtuin gene activator such as NMN is used as the other, the above-mentioned effects can be obtained more significantly than in the case of using either agent alone, and even greater effects can be obtained by combining the two rather than using twice the amount of one agent during the treatment.

[0057] In particular, when the first spray solution using the first penetrating agent and the second spray solution using the second penetrating agent are sprayed separately and in sequence, the active ingredients can be permeated into the skin one by one, and each is permeated in turn by the bloodstream and lymphatic flow, resulting in a synergistic effect. In particular, when this treatment is performed on the face, it can produce a remarkable effect in changing the contours and size of the eyes, and some people may even suspect that they have had cosmetic surgery.

[0058] In addition, since the first spray liquid and the second spray liquid are sprayed by the spray device 5, a fixed amount of spray can be sprayed with a constant intensity each time. Therefore, the same effect can be obtained regardless of the skill of the practitioner, as long as the practitioner sprays the same area in the same order. EXAMPLES

[0059] An embodiment of the multiple component skin penetration method using the multiple component skin penetration system 1 described above will be described below. The first penetrating agent 2, the second penetrating agent 3, and the adjusting agent 4 used in this example are as follows.

[0060] First penetrating agent 2: NMN <Details> β-Nicotinamide Mononucleotide - Grain size: 16 mesh or less ·Bulk specific gravity: 0.6g / ml or more ·Purity 99% or more (composition ratio: 100%) Processing: Micro granulation processing ·Shape: white crystalline powder ·Amount: 500mg Amount used in this example: 1 / 3 (=166.67 mg)

[0061] Second permeation agent 3: Human umbilical cord stem cell culture supernatant <Details> Human mesenchymal stem cells derived from umbilical cord matrix (hMSC-UC) · Proliferative cell culture Passage 2 (third culture) ·Storage method: Cryopreservation ·Amount: 1ml Amount of data in this example: All

[0062] Adjustment agent 4: Purified water - 400,000 times purer than tap water ·Amount: 400ml Amount used in this example: (1) 3 ml is used to mix with 166.67 g of NMN powder as the first penetrant 2. After mixing, about 3.17 ml of the second spray liquid is left. (2) 2 ml is used to mix with 1 ml of human umbilical cord stem cell culture supernatant as the second penetrating agent 3. After mixing, 3 ml of the first spray liquid is added.

[0063] 0. Preparation First, the subjects' skin was pre-cleansed and then massaged to drain lymph nodes on the neck, décolleté, and face as a preliminary step.

[0064] 1. Spraying the first spray liquid (NMN) The first liquid for spraying was sprayed onto the décolleté, neck, and face using the spray device 5. Spraying was performed on each area in turn with the tip of the spray cover 11 in contact with the skin. Spraying was performed approximately 1,000 times in total.

[0065] 2. Spraying of the second liquid for spraying (human umbilical cord stem cell culture supernatant) The second liquid for spraying was sprayed onto the décolleté, neck, and face using the spray device 5. Spraying was performed on each area in turn with the tip of the spray cover 11 in contact with the skin. Spraying was performed approximately 1,000 times in total.

[0066] The above treatment was performed on two patients, and the following results were obtained:

[0067] [Table 1]

[0068] In particular, because the change in the contour is easy to see, an example of the change in subject 1 will be described in Figure 4. Figure 4(A) is a photograph at the stage where the above "0. Preparation" was completed, that is, at the stage where the lymphatic drainage massage was finished. Figure 4(B) is a photograph of the condition after spraying NMN and then spraying human umbilical cord stem cell culture supernatant. FIG. 4(C) is a photograph showing the facial contour of FIG. 4(A) before spraying superimposed on the facial contour of FIG. 4(B) after spraying. As shown in this figure, from the state after lymphatic drainage (Before) to the state after (After), it is clear that significant effects can be obtained by simply performing this treatment once, in which NMN and human stem cell culture medium are applied in sequence.

[0069] Furthermore, the subject was told by a friend the next day that she must have gone for surgery, and a person she met a week after the procedure also said she must have gone for surgery. In other words, this procedure, which involves applying NMN and human stem cell culture fluid in sequence, once can produce significant results, and these results are sustained. EXAMPLES

[0070] In Example 2, only the spraying order was changed from Example 1. That is, the first penetrating agent 2 and the second penetrating agent 3 were exchanged as follows. (1) 2 ml is used to mix with 1 ml of human umbilical cord stem cell culture supernatant as the first penetrating agent 2. After mixing, 3 ml of the first spray liquid is used. (2) 3 ml is used to mix with 166.67 g of NMN powder as the second penetrant 3. After mixing, about 3.17 ml of the second spray liquid is left. Other than this, the procedure was the same as in Example 1, with massage, spraying of the first spray liquid, and spraying of the second spray liquid being carried out in that order. The above treatment was performed on two patients, and the following results were obtained:

[0071] [Table 2]

[0072] In addition, because subject 3 had atopic dermatitis, the first spray liquid (human umbilical cord stem cell culture supernatant) and the second spray liquid (NMN) were sprayed on the atopic areas as well. As a result, the symptoms of the atopic dermatitis improved, and the skin in the atopic areas became more moisturized, firmer, and shinier. EXAMPLES

[0073] Next, the same formulation and treatment as in Example 1 were used to spray onto the scalp, which is a part of the skin, and the hair growth and regrowth effect was confirmed. At this time, the back of the neck and scalp were massaged as a preparatory massage. The other spray liquids and treatment order were the same as in Example 1. Each treatment was counted as one session, and the effects were measured after a total of four treatments, with intervals ranging from one week to one month between each treatment. After performing the treatment on two people, both patients saw clear results in hair growth (scalp skin beauty effects). EXAMPLES

[0074] Next, the same formulation and treatment as in Example 2 were used to spray the scalp, and the hair growth and regrowth effect was confirmed. At this time, the back of the neck and scalp were massaged in advance as a preparatory massage. The other spray liquids and treatment order were the same as in Example 2. Each treatment was counted as one session, and the effects were measured after a total of four treatments, with intervals ranging from one week to one month between each treatment. After performing the treatment on two people, both patients saw clear results in hair growth (scalp skin beauty effects).

[0075] <Examples 5 to 10> In addition, the treatment was administered in various forms and the effects were measured. The contents of the pre-massage, the spraying of the first penetrating agent 2, and the spraying of the second penetrating agent 3 were the same as those in the above-mentioned Examples 3 and 4. In addition, an Example was added in which holes were made in the surface of the skin with a roller (see the penetration promoter 105 described later) before spraying with the spray device 5, and an Example was added in which a shampoo containing proteoglycan was used as a regular shampoo in addition to the treatment. In each Example, a series of treatments was counted as one session, and a total of four treatments were performed, with intervals between each session ranging from one week to one month, and measurements were taken. As a result of the implementation, the results shown in Table 3 below were obtained.

[0076] [Table 3] In all cases, hair growth and thickening effects were confirmed. In particular, Examples 5 to 8, in which both human umbilical cord stem cell culture supernatant and NMN were sprayed in sequence, were highly effective, and among these, Examples 7 and 8, in which a roller was further added, were extremely effective. Also, in Examples 9 and 10, in which a proteoglycan-containing shampoo was used as a regular shampoo, human umbilical cord stem cell culture supernatant was sprayed as the first penetrating agent, and no second penetrating agent was sprayed, an increase in vellus hair was observed.

[0077] The present invention is not limited to the above-described embodiment, but may be embodied in various other forms.

[0078] For example, before spraying the first liquid to be sprayed, holes may be made on the surface of the skin with a roller (see penetration promoter 105 described later) having a plurality of fine needles of a predetermined length arranged on the outer periphery of a cylinder, and then the first liquid to be sprayed and the second liquid to be sprayed may be sprayed in succession. In this case, the penetration of the liquid to be sprayed into the skin can be improved, and a better effect can be obtained. In this case, it is preferable that the needles are made of titanium. The predetermined length can be 0.1 mm to 1 mm, preferably 0.1 to 0.5 mm, and is preferably 0.25 mm.

[0079] In addition, by performing a lymphatic massage as a preliminary step, blood flow and lymph flow can be improved, resulting in better results (massage effect + spray effect), but spraying of the first spray liquid and the second spray liquid may be performed without such preliminary step. Even in this case, a clear change can be seen before and after spraying.

[0080] In addition, the first liquid to be sprayed is sprayed 1,000 times, and the second liquid to be sprayed is sprayed 1,000 times, but this is not limited to the above, and each can be sprayed 500 times, 300 times, or conversely increased to 1,500 times, or other appropriate number of times. The number of sprays depends on the area of ​​the skin to be sprayed, but can be 10 times or more for one type of spray liquid, preferably 50 times or more, more preferably 100 times or more, even more preferably 300 times or more, and preferably 500 times or more. In addition, the upper limit of the number of sprays can be 3,000 times or less for one type of spray liquid, preferably 2,000 times or less, more preferably 1,500 times or less, and preferably 1,000 times or less.

[0081] The area covered by the inside of the tip of the circular spray cover 11 can be applied to 40% or more of the skin area to be treated, preferably 50% or more, more preferably 60% or more, even more preferably 70% or more, more preferably 80% or more, and preferably 90% or more. Although spraying the same position once is sufficient, more active ingredients may be permeated by spraying two or more times. In particular, it is preferable to spray two or more times partially on areas where intensive care is desired, such as fine lines around the eyes, nasolabial folds, and wrinkles on the forehead. This increases the permeation of the active ingredients in the areas where intensive care is desired, and more favorable effects can be obtained in the areas where intensive care is desired.

[0082] In addition, for the spray liquid using the first penetrant 2 and the second penetrant 3, either of the following methods can be used: the first penetrant 2 and the second penetrant 3 are mixed with the adjusting agent 4 and then poured into the liquid storage section 15a to pour all of the components in at the same time; or the first penetrant 2 and the adjusting agent 4 are mixed and poured into the liquid storage section 15a as the first spray liquid and sprayed, and then within a predetermined time, the second penetrant 3 and the adjusting agent 4 are mixed and poured into the liquid storage section 15a as the second spray liquid.

[0083] In the sequential addition of each component, the predetermined time from the completion of spraying of the first spray liquid to the start of spraying of the second spray liquid can be within one week, preferably within 48 hours, preferably within 24 hours, more preferably within 12 hours, even more preferably within 6 hours, even more preferably within 3 hours, even more preferably within 1 hour, even more preferably within 30 minutes, and preferably within 10 minutes. By adding the components sequentially in this manner, the components of the first penetrant 2 are sufficiently permeated, and the components of the second penetrant 3 are also sufficiently permeated, and the effects of both components can be obtained simultaneously, thereby exerting a synergistic effect. In particular, by shortening the predetermined time from the spraying of the first spray liquid (first penetrant 2 + regulator 4) to the spraying of the second spray liquid (second penetrant 3 + regulator 4), the synergistic effect of both components can be enhanced. In addition, by adding them sequentially, each component can be thoroughly permeated rather than being mixed and added at the same time.

[0084] When all ingredients are added at the same time, the overall spray time can be shortened compared to sequential addition of each ingredient. For example, when ingredients are added sequentially, it is necessary to spray the first spray liquid 1,000 times on each part of the face (and the neck and décolleté may be added), and then spray the second spray liquid again 1,000 times on each part of the face (and the neck and décolleté may be added), which takes the time to spray a total of 2,000 times, but when all ingredients are added at the same time, it is possible to spray each part of the face (and the neck and décolleté may be added) only 1,000 times in total, which shortens the time to half.

[0085] In the case of simultaneous injection of all the components, a third penetrant (a third active ingredient) may be mixed in addition to the first penetrant 2 and the second penetrant 3, and then all the components may be injected simultaneously and sprayed. The third penetrant may be any suitable substance, for example, a cannabinoid (third active ingredient) such as CBD (cannabidiol), CBN (cannabinol) or CBG (cannabigenol), and in the case of this cannabinoid, it is preferable that THC (tetrahydrocannabinol) has been removed.

[0086] The third penetrating agent can also be a proteoglycan. In this case, by applying it to the scalp in particular, it can improve the effects on hair (hair growth effect, hair volume increase effect, hair line advancement effect, hair firmness increase effect, hair strength increase effect, hair thickening effect, gray hair turning back to black hair, etc.).

[0087] In the case of sequential addition of components, after spraying the first spray liquid (first penetrant 2 + regulator 4) and the second spray liquid (second penetrant 3 + regulator 4), the third spray liquid (third penetrant + regulator 4) can be sprayed within a predetermined time. Furthermore, it is also possible to add the third spray liquid (fourth penetrant + regulator 4) by spraying it within a predetermined time.

[0088] In addition, the first active ingredient is NMN (sirtuin gene activator), and the second active ingredient is human stem cell culture fluid (growth factor agent), which are applied (sprayed) in that order, but the order may be reversed, with the first active ingredient being human stem cell culture fluid (growth factor agent), and the second active ingredient being NMN (sirtuin gene activator), which are applied (sprayed) in that order. In this case, too, each active ingredient can be penetrated sequentially to exert their respective synergistic effects.

[0089] In addition, one of the first and second active ingredients may be a human stem cell culture medium, and the other may be a proteoglycan, and applied (sprayed) in that order. In this case, too, the effects of each ingredient can be fully exerted to obtain a synergistic effect. In this case, applying the ingredient to the scalp can provide a high effect in promoting hair growth and hair care.

[0090] In addition, the method of application is preferably spraying as described above, but is not limited to this, and other methods such as applying to the skin by hand, soaking in a pack or the like and placing it on the skin, etc. can also be used. In this case, the penetration of the active ingredient can be increased by making multiple small holes in the skin using a fine needle. In this case, a certain degree of effect can also be obtained, and by applying the active ingredients in order, the synergistic effect of each active ingredient can be exerted.

[0091] This first embodiment can be provided as a composition kit (beauty kit, skin external application kit). This composition kit can be a set of the first penetrating agent 2 and the second penetrating agent 3, a set of the first penetrating agent 2, the second penetrating agent 3, and the regulator 4, a set of the first penetrating agent and the regulator 4, a set of the second penetrating agent and the regulator 4, or a set of the first penetrating agent 2, the second penetrating agent 3, the regulator 4, and a spray device 5 (penetration enhancer). In this case, the user can purchase these, adjust the first penetrating agent 2 with the regulator 4 and apply it to the skin with the spray device 5, and then adjust the second penetrating agent 3 with the regulator 4 and apply it to the skin with the spray device 5.

[0092] Similarly, the above-mentioned Examples 4 to 10 can be provided as a composition kit for hair growth and hair restoration, that is, a hair growth and hair restoration kit. This hair growth and hair restoration kit can be a set of the first penetrant 2 and the second penetrant 3, a set of the first penetrant 2, the second penetrant 3, and the regulator 4, a set of the first penetrant and the regulator 4, a set of the second penetrant and the regulator 4, or a set of the first penetrant 2, the second penetrant 3, the regulator 4, and a spray device 5 (penetration promoter). In this case, the user can purchase these, adjust the first penetrant 2 with the regulator 4 and apply it to the skin with the spray device 5, and then adjust the second penetrant 3 with the regulator 4 and apply it to the skin with the spray device 5.

[0093] FIG. 5 is a diagram showing another example of a composition kit. The composition kit 101 includes a first skin topical product 120 containing a first penetrating agent 102 in a first container 125, a second skin topical product 130 containing a second penetrating agent 103 in a second container 135, a liquid storage item 140 containing a liquid 104 serving as an adjusting agent in a liquid storage container 145, and a penetration promoter 105 that promotes the penetration of the beauty ingredient into the skin.

[0094] The first penetrating agent 102 may be the same as the first penetrating agent 2 described above or may contain other components, and the second penetrating agent 103 may be the same as the second penetrating agent 3 described above or may contain other components. The other components to be contained may be appropriate components such as niacinamide, collagen, hyaluronic acid, elastin, retinol (vitamin A), vitamin C, ceramide, amino acids, neil-one, fullerene, honey extract, or a combination of these.

[0095] Niacinamide is also called nicotinamide, D-melano, or wrinkle niacin, and is a type of vitamin B. Niacinamide can be contained at 0.01% or more, preferably at 0.05% or more, more preferably at 0.1% or more, more preferably at 0.5% or more, and even more preferably at 1% or more. Niacinamide can be 70% or less, preferably at 50% or less, more preferably at 30% or less, more preferably at 20% or less, even more preferably at 10% or less, more preferably at 5% or less, and is preferably at 3.5% or less.

[0096] The first penetrant 102 and the second penetrant 103 are preferably in the form of powder that dissolves in liquid such as water, purified water, or saline solution, etc. This allows the first penetrant 102 and the second penetrant 103 to be stored in a stable state and can be used as a liquid when in use.

[0097] The first skin topical product 120 and the second skin topical product 130 are preferably prepared by sterilizing the first container 125 and the second container 135, storing the powdered first penetrating agent 102 and the powdered second penetrating agent 103 in a clean room, and then closing and sealing the lids 123 and 133, respectively. This makes it possible to provide the first skin topical product 120 and the second skin topical product 130 without containing a preservative component.

[0098] The penetration enhancer 105 is a needle roller type beauty tool in which a cylindrical roller 152 is rotatably supported on one end of a rod-shaped handle 153 serving as a handle, and a large number of needles 151 protrude from the surface of the roller 152. More than half of the base side of the needles 151 is embedded in the roller 152, and the tip protrudes from the surface of the roller 152. The lower limit of the protruding length of the needles 151 can be 0.1 mm or more, preferably 0.2 mm or more, and the upper limit can be 1.0 mm or less, preferably 0.7 mm or less, more preferably 0.5 mm or less, even more preferably 0.4 mm or less, and more preferably 0.3 mm or less. In the case of home use, it is preferable to set the length to 0.25 mm within this range. The material of the needles 151 can be a metal material, and is preferably titanium. By making the needles 151 titanium, antibacterial properties can be enhanced. In addition, the needles 151 are arranged at equal intervals on the circumferential outer periphery of the roller 152. The number of needles 151 may be 200 or more at the lower limit, preferably 300 or more, and more preferably 400 or more. The number of needles 151 may be 1,000 or less at the upper limit, preferably 900 or less, and more preferably 800 or less. In this embodiment, 60 needles 151 are arranged in a circle at equal intervals in one row, and 540 needles are arranged in six parallel rows at equal intervals. In this embodiment, the penetration enhancer 105 is a needle roller type beauty tool, but is not limited to this, and the above-mentioned spray device 5 (see FIG. 1) may be used as the penetration enhancer 105.

[0099] The first container 125 and the second container 135 are each composed of a main body 124, 134 made of a glass bottle and a lid 123, 133 that seals the upper opening of the main body 124, 134, and the first penetrant 102 or the second penetrant 103 is contained in the internal space of the main body 124, 134, respectively.

[0100] The size of the main body 124, 134 is a capacity sufficient for dissolving the first penetrant 102 or the second penetrant 103, which is a powder, with the liquid 104. The lower limit of this capacity can be 1 ml or more, preferably 2 ml or more, more preferably 3 ml or more, and more preferably 4 ml or more. The upper limit of this capacity is preferably 20 ml or less, more preferably 15 ml or less, even more preferably 10 ml or less, and more preferably 7 ml or less. In this embodiment, the capacity is 4 ml, and 3 ml of the liquid 104 is poured in to dissolve the first penetrant 102 or the second penetrant 103.

[0101] Next, Examples 11 to 18 in which the composition kit 101 was used by users at home will be described with reference to Table 4. In Examples 11 to 18, the first penetrating agent 102 and the second penetrating agent 103 were each dissolved in 3 ml of purified water (liquid 104) before use.

[0102] In addition, in Examples 11 to 14, a needle roller type beauty tool was used as the penetration enhancer 105, and the first penetrating agent and the second penetrating agent were applied after holes were made in the facial skin with the needle roller type beauty tool. This series of steps was repeated five times, and the effect was measured, with the intervals between each of the five measurements ranging from one week to one month.

[0103] In addition, in Examples 15 to 18, a spray device 5 was used as the permeation enhancer 105, and after the first penetrant was applied (sprayed) by this spray device 5, the second penetrant was applied (sprayed) by the spray device 5. In Examples 15 to 18, the effects of one implementation were measured.

[0104] In addition, since Examples 11 to 18 are for home use, no prior massage was performed. In addition, the first and second penetrating agents in Examples 11 to 18 are respectively used by dissolving 1 mg of human umbilical cord stem cell culture supernatant (freeze-dried powder) in 3 ml of purified water (liquid 104), by dissolving 150 mg of NMN (powder) in 3 ml of purified water (liquid 104), or by dissolving 1 mg of proteoglycan (freeze-dried powder) in 3 ml of purified water (liquid 104).

[0105] [Table 4]

[0106] As shown in Table 4, the effect was observed in both cases. 6 is an explanatory diagram illustrating before and after photographs of Example 16. In this example, a 76-year-old woman was used as the subject.

[0107] Fig. 6(A) is a photograph of the state before starting, Fig. 6(B) is a photograph of the state after NMN (powder) dissolved in purified water was sprayed with spray device 5, and Fig. 6(C) is a photograph of the state after human umbilical cord stem cell culture supernatant agent (freeze-dried powder) dissolved in purified water was sprayed with spray device 5. In this example, spraying of NMN (powder) was carried out over about 15 minutes, and after an interval of about 10 minutes for photography and preparation for the next step, spraying of human umbilical cord stem cell culture supernatant agent (freeze-dried powder) was carried out over about 15 minutes, for a total time of about 40 minutes from start to finish.

[0108] The lifting effect, eye opening, wrinkle improvement, cheek position, skin firmness, skin luster, and whitening all have remarkable effects, and FIG. 6 shows a comparison of the skin from above the mouth to the nose, which is the most obvious result. It can be clearly seen that wrinkles are fewer and shallower from before the treatment in FIG. 6(A) to after NMN in FIG. 6(B). In addition, compared to FIG. 6(B) showing the improved state after spraying NMN (powder) with the spray device 5, FIG. 6(C) showing the state after spraying human umbilical cord stem cell culture supernatant (freeze-dried powder) with the spray device 5 shows further improvement. In this way, by applying the first penetrating agent 102 and the second penetrating agent 103 in sequence, two-stage improvement is obtained, resulting in a highly youthful appearance effect.

[0109] Next, Examples 19 to 57 will be described with reference to Tables 5 and 6. These Examples were carried out using various combinations of sirtuin gene activators and growth factor agents to measure the effects.

[0110] [Table 5]

[0111] [Table 6]

[0112] The sirtuin gene activators used are one or more of NAD+, NMN, 5-deazaflavin, and niacinamide, all of which are used in powder form dissolved in purified water as a solvent.

[0113] The growth factors used are one or more of EGE, EGF, PDGF, exosomes, and human stem cell culture supernatant, all of which are in powder form and dissolved in purified water as a solvent. FGF1 is used as the FGF. The human stem cell culture supernatant is human umbilical cord stem cell culture supernatant (freeze-dried powder) derived from the umbilical cord. In addition, the growth factors EGE, EGF, PDGF, exosomes, and human stem cell culture supernatant are all in powder form produced by freeze-drying and dissolved in purified water as a solvent.

[0114] In Tables 5 and 6, the numerical values ​​in each example indicate the volume, and the symbol "-" indicates that the component was not blended. In more detail, the numbers for growth factors (0.2, 0.5, 1, 2, 5) all indicate the volume in ml. The numbers for sirtuin gene activators (50, 100, 150, 200, 250, 300, 500) all indicate the volume in mg. The numbers (2, 3) for the solvent (purified water) indicate the volume in ml.

[0115] In Examples 19 to 57, no prior massage was performed, and the first penetrant was dissolved in a solvent and sprayed onto the entire facial skin and the skin under the chin using the spray device 5 also used in Example 1. After an interval of about 3 minutes, the second penetrant was dissolved in a solvent and sprayed onto the entire facial skin and the skin under the chin using the spray device 5.

[0116] As described in the effects of each example, improvements were observed in the lifting effect, eye opening, wrinkle reduction, cheek position, skin firmness, skin luster, and skin whitening effects. The example using NAD+ as a sirtuin gene activator showed the highest effect.

[0117] In addition, in Examples 49 to 52, in which a growth factor agent was not used, but NAD+ was used as a sirtuin gene activator, dissolved in purified water, and sprayed onto the skin with the spray device 5, the effect was lower than that of the examples in which a growth factor agent was used, but a certain degree of effect was obtained. In addition, in Examples 49 to 52, NAD+ and a growth factor agent may be mixed and dissolved in purified water and sprayed with the spray device 5. In this case, the growth factor agent may be a growth factor, an exosome, a stem cell culture supernatant agent, or a combination of these, and it is preferable to use exosomes. Even in this case, although the effect is lower than when the growth factor agent and the sirtuin gene activator are applied separately and sequentially, a certain degree of effect can be obtained. Specifically, when 1 mg of exosomes and 200 mg of NAD+ were mixed and dissolved in 3 ml of purified water and sprayed with the spray device 5, significant improvements were observed in the opening of the eyes, wrinkle improvement, skin firmness, skin luster, and whitening, and improvements were observed in the lift-up effect and the position of the cheeks. [Industrial Applicability]

[0118] The present invention can be used in industries such as beauty salons and hair growth salons. [Explanation of symbols]

[0119] 1. Multiple-ingredient skin penetration system 12a…Spray nozzle 15a...Liquid storage section 17...Solenoid 19d…Control section 33...Gas supply section

Claims

1. Applying a first composition containing one or more selected from a sirtuin gene activator, a growth factor agent, a proteoglycan, and a cannabinoid; and applying a second composition containing one or more of the sirtuin gene activator, the growth factor agent, the proteoglycan, and the cannabinoid other than the first composition. Skin beauty treatments (excluding medical procedures).

2. The sirtuin gene activator is an NAD+ precursor, an NAD+ analog, NAD+, niacinamide, or a combination thereof. The skin cosmetic method according to claim 1 (excluding medical procedures).

3. The growth factor agent is a growth factor, an exosome, a stem cell culture supernatant agent, or a combination thereof. The skin beauty method according to claim 2 (excluding medical procedures).

4. The step of applying the composition to the skin is carried out using a penetration device that promotes penetration of the composition into the skin. The skin cosmetic method according to claim 3 (excluding medical procedures).

5. The sirtuin gene activator is applied using a penetration device that promotes penetration into the skin. Skin beauty treatments (excluding medical procedures).

6. Applying a first composition containing one or more selected from a sirtuin gene activator, a growth factor agent, a proteoglycan, and a cannabinoid; and applying a second composition containing one or more of the sirtuin gene activator, the growth factor agent, the proteoglycan, and the cannabinoid other than the first composition. Hair growth and regrowth methods.

7. A first skin care product in which a first composition containing one or more selected from a sirtuin gene activator, a growth factor agent, a proteoglycan, and a cannabinoid is contained in a first container; and a second skin care product in which a second composition containing one or more of the sirtuin gene activator, the growth factor, the proteoglycan, and the cannabinoid other than the first composition is contained in a second container. Composition kit.

8. The first container does not contain the other active ingredient contained in the second composition, The second container does not contain one of the active ingredients contained in the first composition. The composition kit according to claim 7.

9. the first composition is in powder form; The second composition is also in powder form; A liquid container that contains a liquid that dissolves the first composition and the second composition in a third container. The composition kit according to claim 8.

10. A liquid storage section that stores a liquid to be sprayed containing an active ingredient; a spray nozzle for spraying the spray liquid contained in the liquid container; A gas supply unit that supplies a gas; a spray control unit that instantly supplies gas supplied from the gas supply unit to the spray nozzle to spray the spray liquid together with the gas from the spray nozzle, A first penetrant containing a first active ingredient for preparing the liquid for spraying and a second penetrant containing a second active ingredient different from the first active ingredient for preparing the liquid for spraying are adjusted with an adjuster, respectively or together, to form a liquid for spraying. Multiple ingredient skin penetration method.

11. A liquid storage section that stores a liquid to be sprayed containing an active ingredient; a spray nozzle for spraying the spray liquid contained in the liquid container; A gas supply unit that supplies a gas; a spray control unit that instantaneously supplies the gas supplied from the gas supply unit to the spray nozzle to spray the spray liquid from the spray nozzle together with the gas, A first penetrant containing a first active ingredient to prepare the spray liquid; A second penetrant containing a second active ingredient different from the first active ingredient for forming the spray liquid; and an adjuster for adjusting the first penetrant and / or the second penetrant. Multi-ingredient skin penetration system.

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