Joint function improvement agent

Abstract

To provide a joint function improving composition capable of protecting cartilage and improving joint pain.SOLUTION: The joint function improving composition comprises, as active ingredients, specific ingredients from glucosamine and at least one plant material selected from barley, peucedanum japonicum thunb, kudzu, rice, maqui berry and ginger.SELECTED DRAWING: Figure 1

Description

[Technical field]

[0001] The present invention relates to a composition capable of improving joint function, and more particularly to a composition containing glucosamine and other specific ingredients as active ingredients. [Background technology]

[0002] As the population ages, the number of people suffering from joint pain such as knee and elbow pain is increasing, and medicines and the like that can relieve such joint pain are desired. For example, amino sugars such as glucosamine have attracted attention as materials that are effective in improving arthritis, which is one of the causes of joint pain (see Patent Document 1). [Prior art documents] [Patent documents]

[0003] [Patent Document 1] JP 2003-325136 A Summary of the Invention [Problem to be solved by the invention]

[0004] An object of the present invention is to provide a composition capable of protecting cartilage and improving joint function. [Means for solving the problem]

[0005] The present inventors have conducted extensive research and studies on cartilage present in joints in order to improve joint function, and have found that a high cartilage protection effect can be obtained by combining glucosamine with other specific components, which has led to the completion of the present invention. That is, they have found that cartilage can be protected more effectively by combining glucosamine with other specific components that have little or no cartilage protection ability.

[0006] That is, the present invention relates to a composition comprising, as active ingredients, glucosamine and at least one component selected from the group consisting of the following (a) to (f): (a) at least one plant material selected from barley, chou-meisou, kudzu, rice, maqui berry, and ginger; (b) at least one functional additive selected from aspartame, sucrose fatty acid esters, and calcium stearate; (c) at least one amino acid selected from alanine, asparagine, glutamic acid, histidine, isoleucine, leucine, phenylalanine, proline, serine, tyrosine, valine, and S-adenosylmethionine; (d) at least one polyphenol selected from gallic acid and chlorogenic acid; (e) at least one vitamin or mineral selected from vitamin A, vitamin B1, vitamin B2, vitamin B12, vitamin C, vitamin D3, sodium pantothenate, niacin, biotin, vitamin K, β-carotene, calcium, and selenium; (f) at least one cartilage component selected from proteoglycan and type II collagen;

[0007] The composition of the present invention can be used as a joint function improving agent having a cartilage protecting effect.

[0008] The composition of the present invention may be obtained by adding an active ingredient consisting of glucosamine and at least one component selected from the group consisting of (a) to (f).

[0009] The composition of the present invention is preferably for oral administration, and is preferably in the form of a tablet, capsule, powder, granule, or liquid.

[0010] The present invention also relates to a method for improving joint function (excluding medical procedures) which comprises ingesting the composition of the present invention. Effect of the Invention

[0011] The composition of the present invention can protect cartilage and improve joint function. [Brief description of the drawings]

[0012] [Figure 1] FIG. 2 shows the expression level of MMP3 when the composition of the present invention (glucosamine + plant material) was applied to chondrocytes (NHAc-kn). The upper left figure shows the result when barley was used as the plant material, the upper right figure shows the result when chou-meisou grass was used as the plant material, the lower left figure shows the result when kudzu was used as the plant material, and the lower right figure shows the result when rice (rice bran) was used as the plant material. [Diagram 2] FIG. 1 shows the expression level of MMP3 when the composition of the present invention (glucosamine + plant material) was applied to chondrocytes (NHAc-kn). The upper left figure shows the result when maqui berry was used as the plant material, the upper right figure shows the result when red ginger (Zingiber officinale var. Rubra) was used as the plant material, and the lower left figure shows the result when ginger (Zingiber officinale) was used as the plant material. [Diagram 3] FIG. 1 shows the expression level of MMP3 when the composition of the present invention (glucosamine + functional additive) was applied to chondrocytes (NHAc-kn). The upper left figure shows the result when aspartame was used as the functional additive, the upper right figure shows the result when sucrose fatty acid ester was used as the functional additive, and the lower left figure shows the result when calcium stearate was used as the functional additive. [Figure 4] FIG. 1 shows the expression level of MMP3 when the composition of the present invention (glucosamine + amino acids) was applied to chondrocytes (NHAc-kn). The upper left diagram shows the result when alanine was used as the amino acid, the upper right diagram shows the result when asparagine was used as the amino acid, the lower left diagram shows the result when glutamic acid was used as the amino acid, and the lower right diagram shows the result when histidine was used as the amino acid. [Diagram 5]FIG. 1 shows the expression level of MMP3 when the composition of the present invention (glucosamine + amino acids) was applied to chondrocytes (NHAc-kn). The upper left diagram shows the result when isoleucine was used as the amino acid, the upper right diagram shows the result when leucine was used as the amino acid, the lower left diagram shows the result when phenylalanine was used as the amino acid, and the lower right diagram shows the result when proline was used as the amino acid. [Figure 6] FIG. 1 shows the expression level of MMP3 when the composition of the present invention (glucosamine + amino acids) was applied to chondrocytes (NHAc-kn). The upper left diagram shows the result when serine was used as the amino acid, the upper right diagram shows the result when tyrosine was used as the amino acid, the lower left diagram shows the result when valine was used as the amino acid, and the lower right diagram shows the result when S-adenosylmethionine (SAMe) was used as the amino acid. [Figure 7] FIG. 1 shows the expression level of MMP3 when the composition of the present invention (glucosamine + polyphenol) was applied to chondrocytes (NHAc-kn). The left figure shows the result when gallic acid was used as the polyphenol, and the right figure shows the result when chlorogenic acid was used as the polyphenol. [Figure 8] FIG. 1 shows the expression level of MMP3 when the composition of the present invention (glucosamine + vitamins and minerals) was applied to chondrocytes (NHAc-kn). The upper left figure shows the result when vitamin A was used as the vitamin, the upper right figure shows the result when vitamin B1 was used as the vitamin, the lower left figure shows the result when vitamin B2 was used as the vitamin, and the lower right figure shows the result when vitamin B12 was used as the vitamin. [Figure 9] FIG. 1 shows the expression level of MMP3 when the composition of the present invention (glucosamine + vitamins and minerals) was applied to chondrocytes (NHAc-kn). The upper left figure shows the result when vitamin C was used as the vitamin, the upper right figure shows the result when vitamin D3 was used as the vitamin, the lower left figure shows the result when sodium pantothenate was used as the vitamin, and the lower right figure shows the result when niacin was used as the vitamin. [Figure 10]This figure shows the expression level of MMP3 when the composition of the present invention (glucosamine + vitamins and minerals) was applied to chondrocytes (NHAc-kn). The upper left figure shows the result when biotin was used as the vitamin, the upper right figure shows the result when vitamin K was used as the vitamin, and the lower left figure shows the result when β-carotene was used as the vitamin. [Figure 11] This figure shows the expression level of MMP3 when the composition of the present invention (glucosamine + vitamins and minerals) was applied to chondrocytes (NHAc-kn). The upper left figure shows the result when fossil coral Ca was used as the mineral, the upper right figure shows the result when calcined shell Ca was used as the mineral, the lower left figure shows the result when uncalcined shell Ca was used as the mineral, and the lower right figure shows the result when milk Ca was used as the mineral. [Figure 12] FIG. 1 shows the expression level of MMP3 when the composition of the present invention (glucosamine + vitamins and minerals) was applied to chondrocytes (NHAc-kn), and shows the case where selenium was used as the mineral. [Figure 13] FIG. 1 shows the expression level of MMP3 when the composition of the present invention (glucosamine + cartilage component) was applied to chondrocytes (NHAc-kn). The left figure shows the case where proteoglycan was used as the cartilage component, and the right figure shows the case where type II collagen was used as the cartilage component. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0013] The composition of the present invention is characterized by containing, as active ingredients, glucosamine and at least one component selected from the group consisting of the following (a) to (f) (hereinafter sometimes referred to as other component). The following components (a) to (f) used together with glucosamine may be used alone (for example, component (a)), or in combination of two or more. When two or more other components are used in combination, it is preferable to combine other components that have a high synergistic effect with glucosamine.

[0014] The composition of the present invention (composition for improving joint function) can protect cartilage by suppressing destruction of cartilage cells, and can more effectively improve (including prevent) joint pain, allowing the joint function to be fully exerted, and therefore can be used as an agent for improving joint function. Furthermore, the composition for improving joint function of the present invention can protect cartilage throughout the body and improve joint pain throughout the body, but in particular, it can protect cartilage of the lower back, hip joint, and knee joint, and improve pain in these areas, thereby improving joint function.

[0015] [Glucosamine] The glucosamine of the present invention may be glucosamine, its salts, or N-acetylglucosamine. Examples of the salts include hydrochloride, sulfate, and phosphate. Such glucosamine may be derived from a living organism or may be chemically synthesized.

[0016] [Other ingredients] (a) Plant material In the joint function improving composition of the present invention, it is preferable to use at least one plant material selected from barley, chou-mei-sou, kudzu, rice, maqui berry, and ginger as an active ingredient together with glucosamine.

[0017] These plant materials may be any part of the plant, such as leaves, stems, roots, flowers, fruits, trunks, branches, etc., and in addition to the plant materials themselves (including dried products), processed plant materials such as crushed products, juice, extracts, etc. can be used. Examples of crushed products include powders and granules. The juices and extracts may be in liquid form, but can also be used in the form of a paste or dry powder. Extracts can be obtained by extraction using a suitable solvent, and examples of the solvent that can be used include water (hot water), ethanol, and aqueous ethanol. Commercially available plant materials can be used for these plant materials.

[0018] As barley, two-rowed barley, six-rowed barley, etc. can be used. As the plant material of the present invention, stems and leaves are preferred, and young leaves are particularly preferred.

[0019] Chou-mei-sou is a perennial plant also called Peony root, belonging to the Apiaceae family and the genus Peony. The parts of the plant used as the plant material of the present invention are preferably the leaves and roots.

[0020] Kudzu is a creeping perennial plant belonging to the genus Pueraria in the family Fabaceae. The parts of the plant used as the plant material of the present invention are preferably flowers and roots, and more preferably flowers. Kudzu flowers may be collected at any stage from buds to fully opened flowers, or may be mixed and used. The type of kudzu is not particularly limited, but examples include Pueraria thomsonii, Pueraria lobata, Pueraria thunbergiana, etc.

[0021] Rice is a plant of the genus Oryza in the family Poaceae. The parts of the plant used as the plant material of the present invention are preferably the outer husk or embryo of brown rice, and more specifically, it is preferable to use rice bran, which is obtained as a by-product when brown rice is polished. There are no particular limitations on the type of rice, and examples include japonica and indica.

[0022] Maqui berry is a plant of the Portunaceae family native to Patagonia, Chile, and its scientific name is Aristotellia chilensis. The part of the plant used as the plant material of the present invention is preferably the fruit.

[0023] Ginger is a plant of the genus Zingiber in the family Zingiberaceae, and examples thereof include Sanshu ginger and yellow ginger, which have the scientific name Zingiber officinale. Red ginger (local name Jahe merah), which is cultivated in Indonesia and India and has the scientific name Zingiber officinale var. Rubra, can also be preferably used. The part of the plant used as the plant material of the present invention is preferably the rhizome.

[0024] (b) Functional additives In the composition for improving joint function of the present invention, it is preferable to use at least one functional additive selected from aspartame, sucrose fatty acid ester, and calcium stearate as an active ingredient together with glucosamine. Aspartame can be used as a sweetener, and sucrose fatty acid ester and calcium stearate can be used as a lubricant.

[0025] (c) Amino acid In the composition for improving joint function of the present invention, it is preferable to use at least one amino acid (including a salt) selected from alanine, asparagine, glutamic acid, histidine, isoleucine, leucine, phenylalanine, proline, serine, tyrosine, valine, and S-adenosylmethionine as an active ingredient together with glucosamine. Examples of the salt include sodium salt and hydrochloride. (d) Polyphenols In the joint function improving composition of the present invention, it is preferable to use at least one polyphenol selected from gallic acid and chlorogenic acid as an active ingredient together with glucosamine.

[0026] (e) Vitamins and minerals In the composition for improving joint function of the present invention, it is preferable to use, together with glucosamine, at least one vitamin / mineral selected from vitamin A, vitamin B1, vitamin B2, vitamin B12, vitamin C, vitamin D3, sodium pantothenate, niacin, biotin, vitamin K, β-carotene, calcium, and selenium as an active ingredient. Note that calcium and selenium as minerals of the present invention include the form of compounds containing these metals.

[0027] (f) Cartilage component In the joint function improving composition of the present invention, it is preferable to use at least one cartilage component selected from proteoglycan and type II collagen as an active ingredient together with glucosamine.

[0028] Proteoglycan is a complex polysaccharide in which glycosaminoglycans such as chondroitin sulfate and dermatan sulfate are covalently bonded to a protein core, and is abundant in animal tissue, especially cartilage tissue. It is also known that proteoglycan exists in vivo in a structure in which a core protein is further bonded to hyaluronic acid, and its molecular weight is as large as tens of thousands to tens of millions. The proteoglycan used in the present invention can specifically be an animal-derived proteoglycan, and can be a commercially available product.

[0029] Type II collagen is a collagen that is found in large amounts in cartilage. For example, collagen extracted from cartilage of chicken, pig, etc. can be used, and commercially available products can be used.

[0030] The composition for improving joint function of the present invention can be used as a joint function improving agent having a cartilage protecting effect, and such a joint function improving agent is not particularly limited as long as it contains glucosamine and other specified ingredients and can be distinguished from other products as a product in terms of its use in improving joint function. For example, the scope of the present invention includes products that display the function of improving joint function on the main body, packaging, instructions, or advertising materials of the product according to the present invention. Examples of so-called health foods include pharmaceuticals (including quasi-drugs), cosmetics, and functional foods such as specified health foods, nutritional functional foods, and functional foods whose efficacy has been approved by a specified organization. Examples of so-called health foods include those that display the following: "for knee and lower back problems," "for healthy walking," "for light movement," "regulate joint function," "smooth joint movement," "improve joint function," "relieve joint movement problems," "help joint movement," "support joint movement," etc. The joint function improving composition of the present invention can be taken by anyone who is concerned about the above-mentioned effects, regardless of gender or age. However, in order to enjoy the effects of the joint function improving composition of the present invention more effectively, it is preferable for healthy people, excluding those with illnesses, to take it, and it is particularly preferable for elderly people who suffer from the above-mentioned problems to take it.

[0031] The composition for improving joint function of the present invention can be used for oral administration or external administration. There is no particular limitation on the external preparation as long as it is applied to the skin, scalp, etc., and examples of the form include external preparations for the skin such as ointments, creams, gels, lotions, emulsions, packs, and poultices, and injections.

[0032] In addition, when the joint function improving composition of the present invention is used as an oral preparation, its form may be, for example, a tablet, capsule, powder, granule, liquid, granule, bar, plate, block, solid, round, paste, cream, caplet, gel, chewable, stick, etc. Among these, the tablet, capsule, powder, granule, and liquid forms are particularly preferred. Specifically, examples include supplements, container-packed beverages filled in PET bottles, cans, bottles, etc., and instant powdered beverages and instant granular beverages that are dissolved in water (hot water), milk, fruit juice, green juice, etc. and drunk. These are preferred in that they are easy to drink during meals and can increase palatability.

[0033] The content of glucosamine and other ingredients (active ingredients) in the joint function improving composition of the present invention may be appropriately determined within the range in which the desired effect is exhibited.

[0034] In general, when the joint function improving composition of the present invention is a pharmaceutical or supplement (tablet, capsule), the active ingredient is preferably contained in an amount of 0.01 to 100 mass% of the total, calculated as the dry mass, more preferably 0.1 to 85 mass%, and even more preferably 0.5 to 70 mass%.

[0035] When the joint function improving composition of the present invention is a packaged beverage (liquid), the active ingredient is preferably contained in an amount of 0.01 to 15 mass% of the total, calculated as the dry mass, more preferably 0.03 to 12.5 mass%, and even more preferably 0.05 to 10 mass%.

[0036] Furthermore, when the joint function improving composition of the present invention is an instant powdered beverage (powder) or an instant granular beverage (granules), the active ingredient is preferably contained in an amount of 0.1 to 80 mass%, more preferably 0.5 to 70 mass%, and even more preferably 1 to 60 mass%, of the total mass calculated on a dry mass basis.

[0037] In order to more effectively exert the effects of the present invention, the active ingredient is preferably contained in an amount of 80% by mass or more of the entire composition for improving joint function of the present invention (excluding water) in terms of dry mass, more preferably 90% by mass or more, even more preferably 95% by mass or more, and particularly preferably 100% by mass. Furthermore, when the composition for improving joint function of the present invention contains any of the components (a) to (f), it is preferable that the component contained therein is composed only of the active ingredient of the present invention. That is, when the composition for improving joint function of the present invention contains, for example, component (a) (plant material), it is preferable that the composition does not contain any plant material other than barley, chou-mei-sou, kudzu, rice, maqui berry, and ginger.

[0038] The intake amount of the joint function improving composition of the present invention is not particularly limited, but from the viewpoint of more significantly exerting the effect of the present invention, it is preferable to take the amount of the active ingredient per day to be 500 mg / day or more, more preferably to take 800 mg / day or more, and even more preferably to take 1000 mg / day or more. The upper limit is not particularly limited, but is, for example, 5 g / day, preferably 4 g / day. In addition, the daily intake amount of glucosamine hydrochloride is preferably 500 to 4000 mg / day, more preferably 1000 to 3000 mg / day, more preferably 1500 to 2500 mg / day, and particularly preferably 1850 to 2250 mg / day.

[0039] The joint function improving composition of the present invention can be stored as a daily dose in one container or divided into, for example, 2 to 3 multiple containers so that the daily intake amount is the above-mentioned amount.

[0040] The blending mass ratio of glucosamine to other ingredients, calculated on a dry mass basis, is preferably in the range of 0.5:1 to 70:1, more preferably in the range of 0.75:1 to 60:1, even more preferably in the range of 1:1 to 60:1, and particularly preferably in the range of 1:1 to 50:1. By having the blending ratio of glucosamine to other ingredients in the above range, the effects of the present invention can be more effectively exhibited.

[0041] The composition for improving joint function of the present invention can be produced by a known method by adding ingredients other than the active ingredient that are acceptable as an oral or external agent, if necessary.

[0042] In addition, examples of the joint function improving composition as an oral preparation of the present invention include a joint function improving composition containing an active ingredient, as well as a joint function improving composition (joint function improving food) obtained by adding an active ingredient to a food, such as a food in which the content of the active ingredient of the present invention is increased compared to normal foods (including natural foods), or a food in which the active ingredient of the present invention is added to a food that does not normally contain the active ingredient of the present invention. The active ingredient may be added separately or simultaneously, or may be added together with other ingredients other than the active ingredient.

[0043] Examples of the joint function improving food of the present invention include beverages such as carbonated drinks, nutritional drinks, fruit drinks, lactic acid drinks, smoothies, and green juice; cold desserts such as ice cream, ice sorbet, and shaved ice; noodles such as soba, udon, harusame, Chinese noodles, and instant noodles; sweets such as candy, candy, gum, chocolate, candy tablets, snacks, biscuits, jelly, jam, cream, baked goods, and bread; processed seafood and livestock foods such as kamaboko, ham, and sausage; and processed milk and fermented milk. Examples of such products include dairy products such as yogurt; oils and fats such as salad oil, tempura oil, margarine, mayonnaise, shortening, whipped cream, dressing, and processed foods thereof; seasonings such as sauces and soy sauce; and retort pouch foods such as curry, stew, oyakodon, porridge, rice porridge, Chinese rice bowl, katsudon, tempura bowl, beef bowl, hayashi rice, omelet rice, oden, mapo dolphin, gyoza, shumai, hamburger steak, meatballs, various sauces, and various soups.

[0044] The method for improving joint function of the present invention is characterized by ingesting the joint function improving composition of the present invention described above, but does not include medical procedures. The joint function improving composition of the present invention is preferably ingested by healthy people excluding sick people, and is particularly preferably ingested by elderly people who suffer from the above-mentioned problems. An example of the method of the present invention is a method of obtaining a joint function improving effect by providing the joint function improving food of the present invention in a restaurant or other eating and drinking establishment. EXAMPLES

[0045] The present invention will now be described with reference to examples. [Example 1] (1) Chondrocytes (NHAc-kn) at 5 × 10 4 The cells were then prepared in 10% FBS-DMEM at a concentration of 1 × 10 cells / mL, and 200 μL of the mixture was seeded onto a 96-well plate. 4 The number of cells was adjusted to be 100. (2) After pre-incubation for 1-2 days, samples were added as follows. The concentration of each sample was adjusted to 4 times the desired final concentration.

[0046] For water-insoluble samples (other components), they were dissolved in DMSO and then adjusted to a 4x concentration sample in 1% DMSO-10% FBS-DMEM. Ca samples other than calcium chloride were dissolved in 1N HCl and then adjusted to a 4x concentration sample in 1 / 50N HCl-10% FBS-DMEM. IL1b solution was prepared at a ratio of 2μL of 10μg / mL IL1b per 15mL of 10% FBS-DMEM. Commercially available glucosamine hydrochloride was used as glucosamine.

[0047] [Table 1]

[0048] [Table 2]

[0049] [Table 3]

[0050] (3) Approximately 18 hours after the addition of the sample, the cells were harvested using CellAmp Direct RNA Prep Kit for RT-PCR (Takara). Then, the cells were subjected to One Step SYBR PrimeScript PCR. TM The expression level of MMP3 was measured using RT-PCR Kit II. RPLP0 was also measured as an internal standard. A high level of MMP-3 expression indicates that destruction of articular chondrocytes is progressing, whereas a low level of MMP-3 expression indicates that destruction of articular chondrocytes is inhibited and that articular chondrocytes are protected.

[0051] The results are shown in Tables 4 to 10 and FIGS.

[0052] From the left, each graph shows the results for "control," "IL1b added," "addition of glucosamine alone (against addition of IL1b)," "addition of other components alone (against addition of IL1b)," and "addition of glucosamine + other components (against addition of IL1b)." The numbers in the explanation below the graphs indicate the sample concentrations; for example, "G_100" in the graph at the top left of Figure 1 indicates 100 μg / mL of glucosamine. The concentration units of added components are μg / mL in all graphs.

[0053] [Table 4]

[0054] For barley, dried and ground powder of young leaves was used. For Chomeisou, finely ground fresh leaves were used. For kudzu, a hot water extract of the flowers (dried powder) was used. A commercially available rice bran was used. For maqui berry, a commercially available dried powder of the fruit was used. For red ginger (Zingiber officinale var. Rubra), a hot water extract (dried powder) of the rhizome was used. For ginger (Zingiber officinale), a hot water extract (dried powder) of the rhizome was used. For lemon verbena, dried and crushed powder of the leaves was used.

[0055] [Table 5]

[0056] [Table 6]

[0057] [Table 7]

[0058] [Table 8]

[0059] [Table 9]

[0060] [Table 10]

[0061] As shown in Tables 4 to 10 and Figures 1 to 13, the expression level of MMP-3 was synergistically reduced by combining glucosamine with the specific other components of the present invention. Therefore, the joint function improving composition of the present invention can suppress inflammation and protect articular chondrocytes (suppress destruction of articular chondrocytes), thereby more effectively improving joint pain.

[0062] [Example 2] (Production of tablets) Tablets (6 tablets, 3000 mg each) were prepared from the following ingredients:

[0063] Glucosamine hydrochloride 2000mg Proteoglycan 5mg Fossil Coral Ca 150mg Calcium stearate 50mg β-carotene 10mg Vitamin B1 5mg Vitamin B2 5mg Vitamin B12 5mg Vitamin D3 2.5 mg Long life grass end 300mg Proline 30mg Crystalline cellulose 50mg Sucrose fatty acid ester 30mg maltose remainder

[0064] Six tablets are to be taken two or three times a day, either by chewing or by taking without chewing with water.

[0065] [Example 3] (Production of capsules) The following mixture was encapsulated into soft capsules to produce 6 capsules.

[0066] Glucosamine hydrochloride 1500mg Proteoglycan 5mg Rice bran extract 20mg Maqui berry powder 100mg Calcined shell calcium 120mg Vitamin E 30mg Niacin 5mg Chlorogenic acid 10mg Proline 20mg Selenium 5mg Sodium pantothenate 10mg maltose remainder

[0067] Take 6 tablets 2 to 3 times a day with water.

[0068] [Example 4] (Production of granules) The following ingredients were mixed and prepared into granules (3000 mg) in a conventional manner.

[0069] Glucosamine hydrochloride 1800mg Proteoglycan 5mg Uncalcined shell calcium 200mg Sucrose fatty acid ester 50mg Selenium 5mg Vitamin E 50mg Barley grass powder 800mg Niacin 10mg Biotin 10mg Proline 5mg Starch Remaining

[0070] [Example 5] (Production of instant powder) The following ingredients were mixed together and prepared into an instant powder (6 g) in a conventional manner.

[0071] Glucosamine hydrochloride 2500mg Proteoglycan 5mg Uncalcined shell calcium 200mg Barley grass powder 1000mg Long life grass end 500mg Ginger powder 250mg Selenium 5mg Vitamin E 300mg Vitamin K 200mg Niacin 50mg Biotin 50mg Proline 30mg Valine 30mg Leucine 20mg Isoleucine 20mg Aspartame 300mg Oligosaccharides 500mg Dextrin Remainder

[0072] [Example 6] (Production of liquid formulation) A solution (50 mL) consisting of the following components was prepared.

[0073] Glucosamine hydrochloride 2000mg Type II collagen 25mg Milk Ca 200mg Aspartame 800mg Red ginger extract 300mg Ginger extract 200mg Kudzu flower extract 150mg Proline 100mg Asparagine 50mg Valine 25mg Leucine 20mg Isoleucine 20mg Gallic acid 10mg Vitamin C 5mg Fragrance (appropriate amount) water remainder [Industrial Applicability]

[0074] The composition of the present invention has a high effect of improving joint function and can be used as an oral agent or an external agent, and therefore the present invention is highly industrially useful.

Claims

1. An oral composition for improving joint function, comprising glucosamine as an active ingredient for improving joint function, and further comprising at least one amino acid selected from serine, histidine and glutamic acid.

2. An oral composition comprising glucosamine and serine.

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