Soy allergy antigen

Abstract

To provide a novel soy allergy antigen, a method and a kit for diagnosing allergy to soybeans, a pharmaceutical composition containing the antigen, soybeans or processed soybean products containing no such antigen, and a tester for determining the presence / absence of the soy antigen in an object.SOLUTION: A diagnostic kit of the present invention includes at least one of proteins specified as (1) through (8), or at least one of proteins specified as (1α) through (55α).SELECTED DRAWING: None

Description

[Technical field]

[0001] The present invention relates to a novel antigen for soybean allergy. The present invention also relates to a diagnostic kit, diagnostic composition, and diagnostic method for soybean allergy. The present invention also relates to a pharmaceutical composition containing the antigen, and soybeans or soybean processed products in which the antigen has been removed or reduced. The present invention further relates to a tester for determining the presence or absence of a soybean antigen in a subject. [Background technology]

[0002] In the serum and tissues of allergic patients, IgE antibodies specific to specific antigens are produced, and the interaction of these IgE antibodies with specific antigens results in physiological consequences that induce allergic reactions.

[0003] In conventional allergy test drugs, antigen reagents are often prepared by simply grinding food or ingredients that are allergen candidates (Patent Document 1). Thus, conventional antigen reagents do not necessarily contain a specific antigen protein (allergen component) that causes an allergic reaction alone, but contain multiple protein components. Therefore, in conventional antigen reagents, the content of each allergen component is different. For this reason, a positive reaction in an allergy test could be detected only when a protein with a content exceeding a threshold value that can determine a positive reaction in binding with IgE antibodies is an allergen component among the many proteins contained in the conventional antigen reagent. Conversely, for patients whose IgE antibodies bind to allergen components that are contained in small amounts in allergens such as foods, a positive reaction cannot be determined even with conventional allergy test drugs, and the diagnostic efficiency was not sufficiently high.

[0004] In addition, the symptoms and severity of allergic reactions do not necessarily correlate with the amount of allergen components contained in the food or ingredient that is an allergen. Even if a patient's IgE antibodies react to trace amounts of allergen components contained in the food or ingredient that is an allergen, these may cause allergic symptoms or contribute to the severity of the allergic reaction.

[0005] Attempts are being made to improve diagnostic efficiency by examining IgE antibodies against each component that constitutes the crude antigen, thereby distinguishing between sensitization that is directly related to diagnosis and sensitization based on cross-reactivity with panallergens, etc. Regarding soybean allergens, eight are currently registered with the World Health Organization / International Union of Immunology (WHO / IUIS).

[0006] However, in order to increase the reliability of allergy testing, it is necessary to comprehensively identify allergen components in foods and ingredients that are allergen candidates, but the patient detection rate by measuring the above allergen components is still insufficient. Identifying new soy allergens is extremely important not only to increase the accuracy of diagnostic drugs, but also as a target for hypoallergenic foods and therapeutic drugs.

[0007] On the other hand, regarding the separation and purification of proteins, various methods have been considered for separating and purifying proteins and nucleic acids from cell extracts, etc. Dialysis and centrifugation using salt concentration are examples of such methods.

[0008] In addition, many purification methods that utilize the electric charge of protein or nucleic acid residues or the difference in molecular weight are being investigated. Examples of purification methods that utilize electric charge include column chromatography using ion exchange resins and isoelectric focusing. Examples of purification methods that utilize molecular weight differences include centrifugation, column chromatography using molecular weight sieves, and SDS-PAGE (dodecane-sulfuric acid-polyacrylamide gel electrophoresis). An example of such a method is sodium silane sulfate-polyacrylamide gel electrophoresis.

[0009] In recent years, two-dimensional electrophoresis, in which isoelectric focusing is performed in the first dimension and SDS-PAGE is performed in the second dimension, has been used as a method for separating and purifying various proteins from a small amount of sample. The applicants have previously developed two-dimensional electrophoresis methods with high resolution (Patent Documents 2 to 5). [Prior art documents] [Patent documents]

[0010] [Patent Document 1] Patent Publication No. 2002-286716 [Patent Document 2] Patent Publication No. 2011-33544 [Patent Document 3] Patent Publication No. 2011-33546 [Patent Document 4] Patent Publication No. 2011-33547 [Patent Document 5] Patent Publication No. 2011-33548 Summary of the Invention [Problem to be solved by the invention]

[0011] The present invention provides a novel antigen for soybean allergy. The present invention also provides a method and a kit for diagnosing soybean allergy. The present invention also provides a pharmaceutical composition containing the antigen, and soybeans or soybean processed products in which the antigen has been removed or reduced. The present invention further provides a tester for determining the presence or absence of a soybean antigen in a subject. [Means for solving the problem]

[0012] In order to solve the above problems, the present inventors have conducted extensive research into identifying the antigen responsible for soybean allergy. As a result, they have succeeded in identifying a novel antigen to which IgE antibodies in the serum of patients with soybean allergy specifically bind. Based on this finding, the present invention has been completed.

[0013] That is, in one aspect, the present invention may be as follows.

[0014] [1] A diagnostic kit for soybean allergy, comprising at least one of the following proteins (1) to (8): (1) (1A) Bowman-Birk type proteinase inhibitor D-II or a mutant thereof, comprising the following (1A-a) to (1A-b): Any of the proteins in (1A-e): (1A-a) SEQ ID NO: 2 in which one or several amino acids are deleted, substituted, inserted or added a protein comprising the amino acid sequence; (1A-b) an amino acid sequence having an identity of 70% or more with the amino acid sequence shown in SEQ ID NO: 2 Contains protein; (1A-c) One or several nucleotides are deleted, substituted, inserted or added in SEQ ID NO:1 a protein comprising an amino acid sequence encoded by the determined nucleotide sequence; (1A-d) A nucleic acid sequence having an identity of 70% or more with the nucleic acid sequence shown in SEQ ID NO: 1. a protein containing the amino acid sequence to be encoded; or (1A-e) A nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 1 and a strand a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under homogenous conditions; or (1B) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 17 to 20; (2)(2A) Ribulose bisphosphate carboxylase large chain bisphosphate carboxylase large chain) or a variant thereof, Any of the proteins in (2A-e): (2A-a) SEQ ID NO: 4 in which one or several amino acids are deleted, substituted, inserted or added a protein comprising the amino acid sequence; (2A-b) an amino acid sequence having an identity of 70% or more with the amino acid sequence shown in SEQ ID NO: 4 Contains protein; (2A-c) One or several nucleotides are deleted, substituted, inserted or added in SEQ ID NO:3 a protein comprising an amino acid sequence encoded by the determined nucleotide sequence; (2A-d) A nucleic acid sequence having an identity of 70% or more with the nucleic acid sequence shown in SEQ ID NO: 3 is coded. a protein containing the amino acid sequence to be encoded; or (2A-e) A nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 3 and a strand a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under homogenous conditions; or (2B) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 21 to 28; (3) (3A) Gamma-glutamyl hydrolase or a mutant thereof, which is any one of the following proteins (3A-a) to (3A-e): (3A-a) SEQ ID NO: 6 in which one or several amino acids are deleted, substituted, inserted or added a protein comprising the amino acid sequence; (3A-b) An amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO:6 Contains protein; (3A-c) One or several nucleotides are deleted, substituted, inserted or added in SEQ ID NO:5 a protein comprising an amino acid sequence encoded by the determined nucleotide sequence; (3A-d) A nucleic acid sequence having an identity of 70% or more with the nucleic acid sequence shown in SEQ ID NO:5. a protein containing the amino acid sequence to be encoded; or (3A-e) A nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO:5 and a strand a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under homogenous conditions; or (3B) a protein comprising the amino acid sequence represented by SEQ ID NO: 29 or 30; (4) (4A) Guanine nucleotide-binding protein subunit beta-like protein or a mutant thereof, which is any one of the following proteins (4A-a) to (4A-e): (4A-a) SEQ ID NO: 8 in which one or several amino acids are deleted, substituted, inserted or added a protein comprising the amino acid sequence; (4A-b) An amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO: 8 Contains protein; (4A-c) One or several nucleotides are deleted, substituted, inserted or added in SEQ ID NO: 7 a protein comprising an amino acid sequence encoded by the determined nucleotide sequence; (4A-d) A nucleic acid sequence having an identity of 70% or more with the nucleic acid sequence shown in SEQ ID NO: 7 is coded. a protein containing the amino acid sequence to be encoded; or (4A-e) A nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 7 and a strand a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under homogenous conditions; or (4B) a protein comprising the amino acid sequence represented by SEQ ID NO: 31 or 32; (5) (5A) Protein SLE3 or a mutant thereof, which is any one of the following proteins (5A-a) to (5A-e): (5A-a) SEQ ID NO: 10 in which one or several amino acids are deleted, substituted, inserted or added a protein comprising the selected amino acid sequence; (5A-b) An amino acid sequence having 70% or more identity to the amino acid sequence shown in SEQ ID NO: 10 Proteins including; (5A-c) One or several nucleotides are deleted, substituted, inserted or added in SEQ ID NO:9 a protein comprising an amino acid sequence encoded by the determined nucleotide sequence; (5A-d) A nucleic acid sequence having an identity of 70% or more with the nucleic acid sequence shown in SEQ ID NO: 9 is coded. a protein containing the amino acid sequence to be encoded; or (5A-e) A nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 9 and a strand a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under homogenous conditions; or (5B) a protein comprising the amino acid sequence represented by SEQ ID NO: 33 or 34; (6) (6A) Seed biotin-containing protein SBP65 or a mutant thereof, which is any one of the following proteins (6A-a) to (6A-e): (6A-a) SEQ ID NO: 12 in which one or several amino acids are deleted, substituted, inserted or added a protein comprising the selected amino acid sequence; (6A-b) An amino acid sequence having 70% or more identity to the amino acid sequence shown in SEQ ID NO: 12 Proteins including; (6A-c) SEQ ID NO: 11 in which one or several nucleotides are deleted, substituted, inserted or added a protein comprising an amino acid sequence encoded by the added base sequence; (6A-d) A clone having a base sequence having 70% or more identity with the base sequence shown in SEQ ID NO: 11. a protein containing the amino acid sequence to be encoded; or (6A-e) A nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 11 and a sequence a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions; or (6B) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 35 to 53; (7)(7A) Ferritin-2 or a variant thereof, comprising the following (7A- Any of the proteins a) to (7A-e): (7A-a) SEQ ID NO: 14 in which one or several amino acids are deleted, substituted, inserted or added a protein comprising the selected amino acid sequence; (7A-b) An amino acid sequence having 70% or more identity to the amino acid sequence shown in SEQ ID NO: 14 Proteins including; (7A-c) SEQ ID NO: 13 in which one or several nucleotides are deleted, substituted, inserted or added a protein comprising an amino acid sequence encoded by the added base sequence; (7A-d) A clone having a base sequence having 70% or more identity with the base sequence shown in SEQ ID NO: 13. a protein containing the amino acid sequence to be encoded; or (7A-e) A nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 13. a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions; or (7B) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 54 to 61. (8) (8A) Cell division cycle protein 48 homolog or a mutant thereof, which is any one of the following proteins (8A-a) to (8A-e): (8A-a) SEQ ID NO: 16 in which one or several amino acids are deleted, substituted, inserted or added a protein comprising the selected amino acid sequence; (8A-b) An amino acid sequence having 70% or more identity to the amino acid sequence shown in SEQ ID NO: 16 Proteins including; (8A-c) SEQ ID NO: 15 in which one or several nucleotides are deleted, substituted, inserted or added a protein comprising an amino acid sequence encoded by the added base sequence; (8A-d) A clone having a base sequence having 70% or more identity with the base sequence shown in SEQ ID NO: 15. a protein containing the amino acid sequence to be encoded; or (8A-e) A nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 15. a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions; or (8B) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 62 to 68; The diagnostic kit as described above, comprising as an antigen.

[0015] [2] A composition for diagnosing soybean allergy, comprising at least one of the proteins identified as (1) to (8) in [1] above as an antigen.

[0016] [3] A method for providing an indicator for diagnosing soy allergy in a subject, comprising the steps of: (i) contacting a sample obtained from a subject with an antigen, where the sample is a solution containing Ig antibodies; (ii) detecting binding of an IgE antibody to the antigen in a sample obtained from the subject; (iii) detection of binding between the subject's IgE antibodies and the antigen provides an indication that the subject is allergic to soy; wherein the antigen is at least one of the proteins identified as (1) to (8) in [1] above.

[0017] [4] A pharmaceutical composition comprising at least one of the proteins identified as (1) to (8) in [1] above.

[0018] [5] The pharmaceutical composition according to [4] above for treating soybean allergy.

[0019] [6] Soybeans or soybean processed products characterized in that an antigen has been removed or reduced, said antigen being at least one of the proteins identified as (1) to (8) in [1] above.

[0020] [7] A tester for determining the presence or absence of a soybean antigen in a subject, comprising an antibody that binds to at least one of the proteins identified as (1) to (8) in [1] above.

[0021] [8] An antigen derived from soybean, which is at least one of the proteins specified as (1) to (8) in [1] above and which causes allergy to soybean.

[0022] [9] A diagnostic kit for soybean allergy, comprising at least one of the following proteins (1α) to (55α): (1α)(1αA1) endoplasmin homolog isoform X2 or a mutant thereof, which is any one of the following proteins (1αA1-a) to (1αA1-e): (1αA1-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 70; (1αA1-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO: 70; (1αA1-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 69 have been deleted, substituted, inserted or added; (1αA1-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 69; or (1αA1-e) Amino acid encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO:69 Proteins containing sequences; (1αB1) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 71 to 87; (1αA2) endoplasmin homolog isoform X2 or a mutant thereof, which is any one of the following proteins (1αA2-a) to (1αA2-e): (1αA2-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 89; (1αA2-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO: 89; (1αA2-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 88 have been deleted, substituted, inserted or added; (1αA2-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 88; or (1αA2-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 88; or (1αB2) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 90 to 104; (2α)(2αA1) heat shock cognate protein 80 or a mutant thereof, Any of the proteins (2αA1-a) to (2αA1-e): (2αA1-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 106; (2αA1-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO: 106; (2αA1-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 105 have been deleted, substituted, inserted or added; (2αA1-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 105; or (2αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 105; (2αB1) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 107 to 120; (2αA2) heat shock protein 90-1 or a mutant thereof, Any of the proteins a) to (2αA2-e): (2αA2-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 122; (2αA2-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO: 122; (2αA2-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 121 have been deleted, substituted, inserted or added; (2αA2-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 121; or (2αA2-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 121; or (2αB2) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 123 to 137; (2αA3) hypothetical protein GLYMA_02G302500 or a variant thereof, which is any one of the following proteins (2αA3-a) to (2αA3-e): (2αA3-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 139; (2αA3-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO: 139; (2αA3-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 138 have been deleted, substituted, inserted or added; (2αA3-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 138; or (2αA3-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 138; or (2αB3) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 140 to 153; (4α)(4αA)embryo-specific urease isoform X1 or a mutant thereof, Any of the proteins (4αA-a) to (4αA-e): (4αA-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 155; (4αA-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 155; (4αA-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 154 have been deleted, substituted, inserted or added; (4αA-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 154; or (4αA-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 154; or (4αB) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 156 and 157; (5α)(5αA)alpha-1,4 glucan phosphorylase L isozyme, chloroplastic / amyloplastic or a mutant thereof, which is any one of the following proteins (5αA-a) to (5αA-e): (5αA-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 159; (5αA-b) a protein comprising an amino acid sequence having 70% or more identity to the amino acid sequence set forth in SEQ ID NO: 159; (5αA-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 158 have been deleted, substituted, inserted or added; (5αA-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 158; or (5αA-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 158; or (5αB) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 160 and 161; (6α)(6αA1)aconitate hydratase 1 or a mutant thereof, Any of proteins 1-a) to (6αA1-e): (6αA1-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 163; (6αA1-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO: 163; (6αA1-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 162 have been deleted, substituted, inserted or added; (6αA1-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 162; or (6αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 162; (6αB1) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 164 to 167; or (6αA2)aconitate hydratase 1 or a mutant thereof, comprising the following (6αA2-a): ~(6αA2-e) any protein: (6αA2-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 169; (6αA2-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO: 169; (6αA2-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 168 have been deleted, substituted, inserted or added; (6αA2-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 168; or (6αA2-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 168; or (6αB2) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 170 to 172; (7α)(7αA)methionine synthase or a mutant thereof, ) to (7αA-e): (7αA-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 174; (7αA-b) a protein comprising an amino acid sequence having 70% or more identity to the amino acid sequence set forth in SEQ ID NO: 174; (7αA-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 173 have been deleted, substituted, inserted or added; (7αA-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 173; or (7αA-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 173; or (7αB) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 175 to 187; (8α)(8αA) transketolase, chloroplastic or a mutant thereof, which is any one of the following proteins (8αA-a) to (8αA-e): (8αA-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 189; (8αA-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 189; (8αA-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 188 have been deleted, substituted, inserted or added; (8αA-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 188; or (8αA-e) A base sequence complementary to the base sequence shown in SEQ ID NO: 188 a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with the nucleic acid; or (8αB) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 190 to 199; (9α)(9αA)lysine-tRNA ligase, cytoplasmic-like isoform X2 or its mutants Any of the following proteins (9αA-a) to (9αA-e): (9αA-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 201; (9αA-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO: 201; (9αA-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 200 have been deleted, substituted, inserted or added; (9αA-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 200; or (9αA-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 200; or (9αB) a protein comprising the amino acid sequence set forth in SEQ ID NO: 202; (10α)(10αA1) chaperonin CPN60-2, mitochondrial or a mutant thereof , any of the following proteins (10αA1-a) to (10αA1-e): (10αA1-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 204; (10αA1-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO: 204; (10αA1-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 203 have been deleted, substituted, inserted or added; (10αA1-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 203; or (10αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 203; (10αB1) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 205 to 218; (10αA2) chaperonin CPN60-2, mitochondrial-like isoform X1 or its mutants Any of the following proteins (10αA2-a) to (10αA2-e): (10αA2-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 220; (10αA2-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO: 220; (10αA2-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 219 have been deleted, substituted, inserted or added; (10αA2-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 219; or (10αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 219; or (10αB2) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 221 to 231; (11α)(11αA) polyadenylate-binding protein 8 isoform X3 or a mutant thereof, which is any one of the following proteins (11αA-a) to (11αA-e): (11αA-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 233; (11αA-b) a protein comprising an amino acid sequence having 70% or more identity to the amino acid sequence set forth in SEQ ID NO: 233; (11αA-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 232 have been deleted, substituted, inserted or added; (11αA-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 232; or (11αA-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 232; or (11αB) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 234 to 237; (12α)(12αA)pyrophosphate-fructose 6-phosphate 1-phosphotransferase subunit alpha-like or a mutant thereof, which is any one of the following proteins (12αA-a) to (12αA-e): (12αA-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 239; (12αA-b) a protein comprising an amino acid sequence having 70% or more identity to the amino acid sequence set forth in SEQ ID NO: 239; (12αA-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 238 have been deleted, substituted, inserted or added; (12αA-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 238; or (12αA-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 238; or (12αB) a protein comprising the amino acid sequence set forth in SEQ ID NO: 240; A (13α)(13αA1) protein disulfide isomerase-like protein precursor or a mutant thereof, comprising any one of the following proteins (13αA1-a) to (13αA1-e): (13αA1-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 242; (13αA1-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO: 242; (13αA1-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 241 have been deleted, substituted, inserted or added; (13αA1-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 241; or (13αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 241; (13αB1) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 243 to 252; (13αA2) protein disulfide isomerase or a mutant thereof, Any of the proteins αA2-a) to (13αA2-e): (13αA2-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 254; (13αA2-b) a protein comprising an amino acid sequence having 70% or more identity to the amino acid sequence set forth in SEQ ID NO: 254; (13αA2-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 253 have been deleted, substituted, inserted or added; (13αA2-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 253; or (13αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 253; or (13αB2) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 255 to 278; (14α)(14αA1)V-type proton ATPase subunit B 2 or a mutant thereof, which is any one of the following proteins (14αA1-a) to (14αA1-e): (14αA1-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 280; (14αA1-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 280; (14αA1-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 279 have been deleted, substituted, inserted or added; (14αA1-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 279; or (14αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 279; (14αB1) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 281 to 284; (14αA2) V-type proton ATPase subunit B 2 or a mutant thereof, which is any one of the following proteins (14αA2-a) to (14αA2-e): (14αA2-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 286; (14αA2-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 286; (14αA2-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 285 have been deleted, substituted, inserted or added; (14αA2-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 285; or (14αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 285; or (14αB2) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 287 to 289; (15,16α)(15,16αA1)UTP-glucose-1-phosphate uridylyltransferase or a variant thereof, which is any one of the following proteins (15,16αA1-a) to (15,16αA1-e): (15,16αA1-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 291; (15,16αA1-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 291; (15,16αA1-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 290 have been deleted, substituted, inserted or added; (15,16αA1-d) 70% or more identity with the base sequence shown in SEQ ID NO: 290 a protein comprising an amino acid sequence encoded by the nucleotide sequence of (15,16αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 290; (15,16αB1) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 292 to 304; (15,16αA2) hypothetical protein GLYMA_02G241100 or a variant thereof, which is any one of the following proteins (15,16αA2-a) to (15,16αA2-e): (15,16αA2-a) A protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 306; (15,16αA2-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 306; (15,16αA2-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 305 have been deleted, substituted, inserted or added; (15,16αA2-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 305; or (15,16αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 305; or (15,16αB2) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 307 to 318; (17α)(17αA1) guanosine nucleotide diphosphate dissociation inhibitor 2 or a mutant thereof, which is any one of the following (17αA1-a) to (17αA1-e): Protein: (17αA1-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 320; (17αA1-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 320; (17αA1-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 319 have been deleted, substituted, inserted or added; (17αA1-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 319; or (17αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 319; (17αB1) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 321 to 325; (17αA2)rab GDP dissociation inhibitor alpha-like or a variant thereof, Any of the following proteins (17αA2-a) to (17αA2-e): (17αA2-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 327; (17αA2-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 327; (17αA2-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 326 have been deleted, substituted, inserted or added; (17αA2-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 326; or (17αA2-e) A sequence complementary to the base sequence shown in SEQ ID NO: 326 a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid comprising (17αB2) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 328 to 332; (18α)(18αA)hypothetical protein GLYMA_10G028300 or a variant thereof, which is any one of the following proteins (18αA-a) to (18αA-e): (18αA-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 334; (18αA-b) a protein comprising an amino acid sequence having 70% or more identity to the amino acid sequence set forth in SEQ ID NO: 334; (18αA-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 333 have been deleted, substituted, inserted or added; (18αA-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 333; or (18αA-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 333; or (18αB) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 335 to 339; (19α)(19αA) sucrose binding protein homolog S-64 or a variant thereof, which is any one of the following proteins (19αA-a) to (19αA-e): (19αA-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 341; (19αA-b) a protein comprising an amino acid sequence having 70% or more identity to the amino acid sequence set forth in SEQ ID NO: 341; (19αA-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 340 have been deleted, substituted, inserted or added; (19αA-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 340; or (19αA-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 340; or (19αB) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 342 to 346; (25α)(25αA1) succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial or a mutant thereof, which is any one of the following (25αA1-a) to (25αA1-e): One of the proteins: (25αA1-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 348; (25αA1-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 348; (25αA1-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 347 have been deleted, substituted, inserted or added; (25αA1-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 347; or (25αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 347; (25αB1) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 349 to 356; (25αA2)succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial or is a mutant thereof, which is any one of the following proteins (25αA2-a) to (25αA2-e): (25αA2-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 358; (25αA2-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 358; (25αA2-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 357 have been deleted, substituted, inserted or added; (25αA2-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 357; or (25αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 357; or (25αB2) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 359 to 365; (26α)(26αA1)phosphoglycerate kinase, cytosolic or a mutant thereof, which is any one of the following proteins (26αA1-a) to (26αA1-e): (26αA1-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 367; (26αA1-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 367; (26αA1-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 366 have been deleted, substituted, inserted or added; (26αA1-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 366; or (26αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 366; (26αB1) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 368 to 382; (26αA2) phosphoglycerate kinase, cytosolic or a mutant thereof, which is any one of the following proteins (26αA2-a) to (26αA2-e): (26αA2-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 384; (26αA2-b) a protein comprising an amino acid sequence having 70% or more identity to the amino acid sequence set forth in SEQ ID NO: 384; (26αA2-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 383 have been deleted, substituted, inserted or added; (26αA2-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 383; or (26αA2-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 383; (26αB2) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 385 to 392; (26αA3) phosphoglycerate kinase, cytosolic or a mutant thereof, which is any one of the following proteins (26αA3-a) to (26αA3-e): (26αA3-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 394; (26αA3-b) a protein comprising an amino acid sequence having 70% or more identity to the amino acid sequence set forth in SEQ ID NO: 394; (26αA3-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 393 have been deleted, substituted, inserted or added; (26αA3-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 393; or (26αA3-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 393; or (26αB3) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 395 to 409; (27α)(27αA1) alcohol dehydrogenase 1 or a mutant thereof, Any of the proteins 27αA1-a) to (27αA1-e): (27αA1-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 411; (27αA1-b) a protein comprising an amino acid sequence having 70% or more identity to the amino acid sequence set forth in SEQ ID NO: 411; (27αA1-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 410 have been deleted, substituted, inserted or added; (27αA1-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 410; or (27αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 410; (27αB1) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 412 to 416; (27αA2) alcohol dehydrogenase 1 or a mutant thereof, Any of the proteins 2-a) to (27αA2-e): (27αA2-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 418; (27αA2-b) a protein comprising an amino acid sequence having 70% or more identity to the amino acid sequence set forth in SEQ ID NO: 418; (27αA2-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 417 have been deleted, substituted, inserted or added; (27αA2-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 417; or (27αA2-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 417; (27αB2) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 419 and 420; (27αA3) alcohol dehydrogenase 1-like or a variant thereof, which is any one of the following proteins (27αA3-a) to (27αA3-e): (27αA3-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 422; (27αA3-b) a protein comprising an amino acid sequence having 70% or more identity to the amino acid sequence set forth in SEQ ID NO: 422; (27αA3-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 421 have been deleted, substituted, inserted or added; (27αA3-d) A base sequence having 70% or more identity to the base sequence shown in SEQ ID NO: 421 a protein comprising the amino acid sequence encoded by the sequence; or (27αA3-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 421; or (27αB3) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 423 to 427; (28α)(28αA)35 kDa seed maturation protein isoform X1 or its mutants Any of the following proteins (28αA-a) to (28αA-e): (28αA-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 429; (28αA-b) a protein comprising an amino acid sequence having 70% or more identity to the amino acid sequence set forth in SEQ ID NO: 429; (28αA-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 428 have been deleted, substituted, inserted or added; (28αA-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 428; or (28αA-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 428; or (28αB) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 430 to 433; (29-33α)(29-33αA1) glyceraldehyde-3-phosphate dehydrogenase isoform X1 or a mutant thereof, Any of the proteins in 1-e): (29-33αA1-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 435; (29-33αA1-b) a protein comprising an amino acid sequence having 70% or more identity to the amino acid sequence set forth in SEQ ID NO: 435; (29-33αA1-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 434 have been deleted, substituted, inserted or added; (29-33αA1-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 434; or (29-33αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 434; (29-33αB1) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 436 to 439; (29-33αA2)glyceraldehyde-3-phosphate dehydrogenase, cytosolic or its A mutant of any of the following proteins (29-33αA2-a) to (29-33αA2-e): (29-33αA2-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 441; (29-33αA2-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 441; (29-33αA2-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 440 have been deleted, substituted, inserted or added; (29-33αA2-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 440; or (29-33αA2-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 440; (29-33αB2) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 442 to 445; (29-33αA3) uncharacterized protein LOC100782924 or a mutant thereof, which is any one of the following proteins (29-33αA3-a) to (29-33αA3-e): (29-33αA3-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 447; (29-33αA3-b) a protein comprising an amino acid sequence having 70% or more identity to the amino acid sequence set forth in SEQ ID NO: 447; (29-33αA3-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 446 have been deleted, substituted, inserted or added; (29-33αA3-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 446; or (29-33αA3-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 446; or (29-33αB3) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 448 to 451; (34α)(34αA1) A bifunctional UDP-glucose 4-epimerase and UDP-xylose 4-epimerase 1-like or a variant thereof, which is any one of the following proteins (34αA1-a) to (34αA1-e): (34αA1-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 453; (34αA1-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 453; (34αA1-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 452 have been deleted, substituted, inserted or added; (34αA1-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 452; or (34αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 452; (34αB1) a protein comprising the amino acid sequence set forth in SEQ ID NO: 454; (34αA2) uncharacterized protein LOC100803483 or a mutant thereof, which is any one of the following proteins (34αA2-a) to (34αA2-e): (34αA2-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 456; (34αA2-b) a protein comprising an amino acid sequence having 70% or more identity to the amino acid sequence set forth in SEQ ID NO: 456; (34αA2-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 455 have been deleted, substituted, inserted or added; (34αA2-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 455; or (34αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 455; or (34αB2) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 457 and 458; (35α)(35αA1) elongation factor 1-alpha or a variant thereof, comprising: Any of the proteins (35αA1-a) to (35αA1-e): (35αA1-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 460; (35αA1-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 460; (35αA1-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 459 have been deleted, substituted, inserted or added; (35αA1-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 459; or (35αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 459; (35αB1) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 461 to 466; (35αA2) elongation factor 1-alpha or a variant thereof, Any of the proteins A2-a) to (35αA2-e): (35αA2-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 468; (35αA2-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 468; (35αA2-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 467 have been deleted, substituted, inserted or added; (35αA2-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 467; or (35αA2-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 467; (35αB2) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 469 to 471; (35αA3) elongation factor-1A isoform X1 or a mutant thereof, Any of the proteins 5αA3-a) to (35αA3-e): (35αA3-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 473; (35αA3-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 473; (35αA3-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 472 have been deleted, substituted, inserted or added; (35αA3-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 472; or (35αA3-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 472; or (35αB3) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 474 to 477; (36,37α)(36,37αA1) seed maturation protein PM34 or a mutant thereof, which is any one of the following proteins (36,37αA1-a) to (36,37αA1-e): (36,37αA1-a) A protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 479; (36,37αA1-b) a protein comprising an amino acid sequence having 70% or more identity to the amino acid sequence set forth in SEQ ID NO: 479; (36,37αA1-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 478 have been deleted, substituted, inserted or added; (36,37αA1-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 478; or (36,37αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 478; (36,37αB1) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 480 to 483; (36,37αA2) uncharacterized protein LOC100809384 or a mutant thereof, which is any one of the following proteins (36,37αA2-a) to (36,37αA2-e): (36,37αA2-a) A protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 485; (36,37αA2-b) a protein comprising an amino acid sequence having 70% or more identity to the amino acid sequence set forth in SEQ ID NO: 485; (36,37αA2-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 484 have been deleted, substituted, inserted or added; (36,37αA2-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 484; or (36,37αA2-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 484; (36,37αB2) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 486 and 487; (36,37αA3) hypothetical protein GLYMA_10G155300 or a variant thereof, which is any one of the following proteins (36,37αA3-a) to (36,37αA3-e): (36,37αA3-a) A protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 489; (36,37αA3-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 489; (36,37αA3-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 488 have been deleted, substituted, inserted or added; (36,37αA3-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 488; or (36,37αA3-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 488; or (36,37αB3) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 490 to 493; (38α)(38αA)seed biotin-containing protein SBP65-like isoform X1 or a mutant thereof, which is any one of the following proteins (38αA-a) to (38αA-e): (38αA-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 495; (38αA-b) a protein comprising an amino acid sequence having 70% or more identity to the amino acid sequence set forth in SEQ ID NO: 495; (38αA-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 494 have been deleted, substituted, inserted or added; (38αA-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 494; or (38αA-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 494; or (38αB) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 496 to 513; (39α)(39αA)ATP synthase subunit O, mitochondrial-like or a mutant thereof, which is any one of the following proteins (39αA-a) to (39αA-e): (39αA-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 515; (39αA-b) a protein comprising an amino acid sequence having 70% or more identity to the amino acid sequence set forth in SEQ ID NO: 515; (39αA-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 514 have been deleted, substituted, inserted or added; (39αA-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 514; or (39αA-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 514; or (39αB) a protein comprising the amino acid sequence set forth in SEQ ID NO:516; (40,56α)(40,56αA)P24 oleosin isoform A or a mutant thereof, Any of the following proteins (40,56αA-a) to (40,56αA-e): (40,56αA-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO:518; (40,56αA-b) a protein containing an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 518; (40,56αA-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO:517 have been deleted, substituted, inserted or added; (40,56αA-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 517; or (40,56αA-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 517; or (40,56αB) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 519 and 520; (41α)(41αA)uncharacterized protein At3g49720-like isoform X2 or its A mutant of any of the following proteins (41αA-a) to (41αA-e): (41αA-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 522; (41αA-b) a protein comprising an amino acid sequence having 70% or more identity to the amino acid sequence set forth in SEQ ID NO: 522; (41αA-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 521 have been deleted, substituted, inserted or added; (41αA-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 521; or (41αA-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 521; or (41αB) a protein comprising the amino acid sequence set forth in SEQ ID NO: 523; (42,43α)(42,43αA1)superoxide dismutase [Mn], mitochondrial or a mutant thereof, which is any one of the following proteins (42,43αA1-a) to (42,43αA1-e): (42,43αA1-a) A protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO:525; (42,43αA1-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO:525; (42,43αA1-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO:524 have been deleted, substituted, inserted or added; (42,43αA1-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 524; or (42,43αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO:524; (42,43αB1) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 526 to 531; (42,43αA2) hypothetical protein GLYMA_04G221300 or a variant thereof, which is any one of the following proteins (42,43αA2-a) to (42,43αA2-e): (42,43αA2-a) A protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO:533; (42,43αA2-b) a protein comprising an amino acid sequence having 70% or more identity to the amino acid sequence set forth in SEQ ID NO:533; (42,43αA2-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO:532 have been deleted, substituted, inserted or added; (42,43αA2-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 532; or (42,43αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 532; or (42, 43αB2) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 534 to 539; (45α)(45αA1)uncharacterized protein LOC100499771 or a mutant thereof, which is any one of the following proteins (45αA1-a) to (45αA1-e): (45αA1-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 541; (45αA1-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 541; (45αA1-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 540 have been deleted, substituted, inserted or added; (45αA1-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 540; or (45αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 540; (45αB1) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 542 to 545; (45αA2) hypothetical protein GLYMA_09G192800 or a variant thereof, which is any one of the following proteins (45αA2-a) to (45αA2-e): (45αA2-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 547; (45αA2-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 547; (45αA2-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 546 have been deleted, substituted, inserted or added; (45αA2-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 546; or (45αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 546; or (45αB2) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 548 to 551; (46α)(46αA) seed maturation protein PM22 or a mutant thereof, which is any one of the following proteins (46αA-a) to (46αA-e): (46αA-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 553; (46αA-b) a protein comprising an amino acid sequence having 70% or more identity to the amino acid sequence set forth in SEQ ID NO: 553; (46αA-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 552 have been deleted, substituted, inserted or added; (46αA-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 552; or (46αA-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 552; or (46αB) a protein comprising the amino acid sequence set forth in SEQ ID NO: 554; (47α)(47αA1)eukaryotic translation initiation factor 5A-2 or its variants A variant of any of the following proteins (47αA1-a) to (47αA1-e): (47αA1-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 556; (47αA1-b) a protein comprising an amino acid sequence having 70% or more identity to the amino acid sequence set forth in SEQ ID NO: 556; (47αA1-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 555 have been deleted, substituted, inserted or added; (47αA1-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 555; or (47αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 555; (47αB1) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 557 and 558; (47αA2) uncharacterized protein LOC100305510 or a mutant thereof, which is any one of the following proteins (47αA2-a) to (47αA2-e): (47αA2-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 560; (47αA2-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 560; (47αA2-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 559 have been deleted, substituted, inserted or added; (47αA2-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 559; or (47αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 559; or (47αB2) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 561 to 563; (48α)(48αA)uncharacterized protein LOC100306570 or a mutant thereof, which is any one of the following proteins (48αA-a) to (48αA-e): (48αA-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 565; (48αA-b) a protein comprising an amino acid sequence having 70% or more identity to the amino acid sequence set forth in SEQ ID NO: 565; (48αA-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 564 have been deleted, substituted, inserted or added; (48αA-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 564; or (48αA-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 564; or (48αB) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 566 to 570; (50α)(50αA)uncharacterized protein LOC100813859 or a variant thereof, which is any one of the following proteins (50αA-a) to (50αA-e): (50αA-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 572; (50αA-b) a protein containing an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 572; (50αA-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 571 have been deleted, substituted, inserted or added; (50αA-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 571; or (50αA-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 571; or (50αB) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 573 to 576; (51α)(51αA)14-3-3-like protein or a mutant thereof, Any of the proteins Aa) to (51αA-e): (51αA-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 578; (51αA-b) an amino acid sequence having 70% or more identity with the amino acid sequence shown in SEQ ID NO: 578 A protein containing an amino acid sequence; (51αA-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 577 have been deleted, substituted, inserted or added; (51αA-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 577; or (51αA-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 577; or (51αB) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 579 to 582; (52α)(52αA)probable fructokinase-4 or a variant thereof, Any of the proteins 2αA-a) to (52αA-e): (52αA-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 584; (52αA-b) a protein comprising an amino acid sequence having 70% or more identity to the amino acid sequence set forth in SEQ ID NO: 584; (52αA-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 583 have been deleted, substituted, inserted or added; (52αA-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 583; or (52αA-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 583; or (52αB) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 585 to 588; (53α)(53αA1)triosephosphate isomerase isoform X1 or a mutant thereof, which is any one of the following proteins (53αA1-a) to (53αA1-e): (53αA1-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 590; (53αA1-b) a protein comprising an amino acid sequence having 70% or more identity to the amino acid sequence set forth in SEQ ID NO: 590; (53αA1-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 589 have been deleted, substituted, inserted or added; (53αA1-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 589; or (53αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 589; (53αB1) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 591 to 597; (53αA2) triosephosphate isomerase, cytosolic or a mutant thereof, which is any one of the following proteins (53αA2-a) to (53αA2-e): (53αA2-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 599; (53αA2-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 599; (53αA2-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 598 have been deleted, substituted, inserted or added; (53αA2-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 598; or (53αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 598; or (53αB2) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 600 to 604; (54α)(54αA1)superoxide dismutase [Fe], chloroplastic isoform X1 or The mutant is any one of the following proteins (54αA1-a) to (54αA1-e): (54αA1-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 606; (54αA1-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 606; (53αA1-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 605 have been deleted, substituted, inserted or added; (54αA1-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 605; or (54αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 605; (54αB1) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 607 to 611; (54αA2) iron-superoxide dismutase or a mutant thereof, Any of the proteins 2-a) to (54αA2-e): (54αA2-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 613; (54αA2-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 613; (54αA2-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 612 have been deleted, substituted, inserted or added; (54αA2-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 612; or (54αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 612; or (54αB2) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 614 to 616; or (55α)(55αA1) 51 kDa seed maturation protein precursor or a mutant thereof, which is any one of the following proteins (55αA1-a) to (55αA1-e): (55αA1-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 618; (55αA1-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 618; (55αA1-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 617 have been deleted, substituted, inserted or added; (55αA1-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 617; or (55αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 617; (55αB1) A small number of amino acid sequences selected from the group consisting of SEQ ID NOs: 619 to 635 Proteins containing at least one; (55αA2)Lea protein precursor or a mutant thereof, Any of the proteins a) to (55αA2-e): (55αA2-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 637; (55αA2-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 637; (55αA2-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 636 have been deleted, substituted, inserted or added; (55αA2-d) a protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more to the nucleotide sequence shown in SEQ ID NO: 636; or (55αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 636; or (55αB2) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 638 to 649; The diagnostic kit as described above, comprising as an antigen.

[0023]

[10] A composition for diagnosing soybean allergy, comprising at least one of the proteins identified as (1α) to (55α) in [9] above as an antigen.

[0024]

[11] A method for providing an indicator for diagnosing soy allergy in a subject, comprising the steps of: (i) contacting a sample obtained from a subject with an antigen, where the sample is a solution containing Ig antibodies; (ii) detecting binding of an IgE antibody to the antigen in a sample obtained from the subject; (iii) detection of binding between the subject's IgE antibodies and the antigen provides an indication that the subject is allergic to soy; wherein the antigen is at least one of the proteins identified as (1α) to (55α) in [9] above.

[0025]

[12] A pharmaceutical composition comprising at least one of the proteins identified as (1α) to (55α) in [9] above.

[13] The pharmaceutical composition according to

[12] above for treating soybean allergy.

[0026]

[14] Soybeans or soybean processed products characterized in that an antigen has been removed or reduced, said antigen being at least one of the proteins identified as (1α) to (55α) in [9] above.

[0027]

[15] A tester for determining the presence or absence of a soybean antigen in a subject, characterized in that it contains an antibody that binds to at least one of the proteins identified as (1α) to (55α) in [9] above.

[0028]

[16] An antigen derived from soybean, which is at least one of the proteins specified as (1α) to (55α) in [9] above, and which causes allergy to soybean. Effect of the Invention

[0029] According to the present invention, a novel antigen for soybean allergy can be provided. Since a novel antigen (allergen component) that causes soybean allergy has been identified in soybeans, it is possible to provide a highly sensitive diagnostic method and diagnostic kit for soybean allergy, a pharmaceutical composition containing the antigen, and soybeans or soybean processed products in which the antigen has been removed or reduced. [Brief description of the drawings]

[0030] [Figure 1] Figure 1 is a photograph of a gel showing the migration pattern of proteins contained in soybeans by two-dimensional electrophoresis. The bands on the left side of the photograph are molecular weight marker bands. [Diagram 2] FIG. 2 is a photograph of an immunoblot using serum from a healthy subject against the two-dimensional electrophoresis pattern of proteins contained in soybean. [Figure 3A] 3A is a photograph of an immunoblot using the serum of soybean-allergic patient 1 against the two-dimensional electrophoresis pattern of proteins contained in soybean. The arrow indicates the spot where the serum of the soybean-allergic patient reacted specifically. [Figure 3B] 3B is a photograph of an immunoblot using the serum of soybean-allergic patient 2 against the two-dimensional electrophoresis pattern of proteins contained in soybean. The arrow indicates the spot where the serum of the soybean-allergic patient reacted specifically. [Figure 3C] 3C is a photograph of an immunoblot using the serum of soybean-allergic patient 3 against the two-dimensional electrophoresis pattern of proteins contained in soybean. The arrow indicates the spot where the serum of the soybean-allergic patient reacted specifically. [Figure 3D]3D is a photograph of an immunoblot using the serum of soybean-allergic patient 4 against the two-dimensional electrophoresis pattern of proteins contained in soybean. The arrow indicates the spot where the serum of the soybean-allergic patient reacted specifically. [Figure 4] FIG. 4 is a photograph showing the two-dimensional electrophoresis pattern of proteins contained in soybean, and an immunoblot using serum from a healthy subject. [Figure 5A] Figure 5A is a photograph of an immunoblot using the serum of soybean allergy patient 1α against the two-dimensional electrophoresis pattern of proteins contained in soybean. The spots shown with white lines or surrounded by squares indicate spots to which the serum of the soybean allergy patient reacted specifically. The number (n) indicates the number of each spot, and each corresponds to the spot specified as "nα" in this specification. [Figure 5B] Figure 5B is a photograph of an immunoblot using the serum of soybean allergy patient 2α against the two-dimensional electrophoresis pattern of proteins contained in soybean. The spots shown with white lines or surrounded by squares indicate spots to which the serum of the soybean allergy patient reacted specifically. The number (n) indicates the number of each spot, and each corresponds to the spot specified as "nα" in this specification. [Figure 5C] Figure 5C is a photograph of an immunoblot using the serum of soybean allergy patient 3α against the two-dimensional electrophoresis pattern of proteins contained in soybeans. The spots shown with white lines or surrounded by squares indicate spots to which the serum of the soybean allergy patient reacted specifically. The number (n) indicates the number of each spot, and each corresponds to the spot specified as "nα" in this specification. [Figure 5D] Figure 5D is a photograph of an immunoblot using the serum of soybean allergy patient 4α against the two-dimensional electrophoresis pattern of proteins contained in soybeans. The spots indicated by white lines indicate spots to which the serum of the soybean allergy patient reacted specifically. The numbers (n) indicate the number of each spot, and each corresponds to the spot specified as "nα" in this specification. [Figure 5E]Figure 5E is a photograph of an immunoblot using serum from a soybean allergy patient 5α against a two-dimensional electrophoresis pattern of proteins contained in soybeans. The spots indicated by white lines indicate spots to which the serum from the soybean allergy patient reacted specifically. The numbers (n) indicate the number of each spot, and each corresponds to the spot identified as "nα" in this specification. [Figure 5F] Figure 5F is a photograph of an immunoblot using serum from soybean allergy patient 6α against a two-dimensional electrophoresis pattern of proteins contained in soybeans. The spots shown with white lines or surrounded by squares indicate spots to which the serum from the soybean allergy patient reacted specifically. The number (n) indicates the number of each spot, and each corresponds to the spot specified as "nα" in this specification. [Figure 5G] Figure 5G is a photograph of an immunoblot using serum from soybean allergy patient 7α against a two-dimensional electrophoresis pattern of proteins contained in soybeans. The spots surrounded by squares indicate spots to which the serum from the soybean allergy patient reacted specifically. The numbers (n) indicate the numbers of each spot, and each corresponds to the spot identified as "nα" in this specification. [Figure 5H] Figure 5H is a photograph of an immunoblot using serum from soybean allergy patient 8α against a two-dimensional electrophoresis pattern of proteins contained in soybean. The spots shown with white lines or surrounded by squares indicate spots to which the serum from the soybean allergy patient reacted specifically. The number (n) indicates the number of each spot, and each corresponds to the spot specified as "nα" in this specification. [Figure 5I] Figure 5I is a photograph of an immunoblot using serum from soybean allergy patient 9α against a two-dimensional electrophoresis pattern of proteins contained in soybean. The spots shown with white lines or surrounded by squares indicate spots to which the serum from the soybean allergy patient reacted specifically. The number (n) indicates the number of each spot, and each corresponds to the spot specified as "nα" in this specification. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0031] The present invention will be described in detail below, but the present invention is not limited thereto.

[0032] Unless otherwise defined herein, scientific and technical terms used in connection with the present invention shall have the meanings that are commonly understood by those of ordinary skill in the art.

[0033] When an amino acid sequence is shown in this specification, it is written in the single-letter code for amino acids well known to those skilled in the art, with the left end being the amino terminus and the right end being the carboxy terminus.

[0034] In this specification, allergy refers to a state in which an organism exhibits an adverse hypersensitive reaction when an antigen that has been sensitized to the organism is re-introduced to the organism. In many food allergies, IgE antibodies specific to the antigen are produced in the blood and tissues. The IgE antibodies bind to mast cells or basophils. When an antigen specific to the IgE antibodies re-enters the body of an allergic disease patient, the antigen combines with the IgE antibodies bound to mast cells or basophils, and the IgE antibodies cross-link on the cell surface, resulting in the physiological effects of IgE antibody-antigen interaction. These physiological effects include the release of histamine, serotonin, heparin, eosinophil chemotactic factor, various leukotrienes, etc. These released substances induce allergic reactions caused by the combination of IgE antibodies and specific antigens. Allergic reactions caused by specific antigens appear through the above-mentioned pathway.

[0035] In this specification, soybean allergy refers to a state in which an allergic reaction occurs due to an antigen such as a protein contained in soybeans. An allergy to soybeans can occur when coming into contact with an antigen contained in soybeans or when the antigen is ingested. In general, an allergic reaction that occurs when food is ingested is specifically called a food allergy. The allergy to soybeans may be a food allergy.

[0036] An antigen, as used herein, is a substance that induces an allergic reaction and is also called an allergen component. Antigens are preferably proteins.

[0037] In the present specification, a protein is a molecule having a structure in which natural amino acids are linked by peptide bonds. The number of amino acids contained in a protein is not particularly limited, but a protein in which about 2 to 50 amino acids are linked by peptide bonds may be called a peptide. In addition, when an amino acid may have optical isomers, the L-isomer is indicated unless otherwise specified. The notation method of the amino acid sequence of a protein or peptide used in the present specification is based on standard usage and the notation method commonly used in the art, and is represented by one-letter notation of amino acids, with the left direction being the amino terminal direction and the right direction being the carboxy terminal direction.

[0038] Identification of antigens The antigens that cause soybean allergies were identified using the above method for proteins contained in soybeans. Specifically, soybean proteins were subjected to two-dimensional electrophoresis under the following conditions.

[0039] The first dimension electrophoresis was performed using a gel for isoelectric focusing, with a gel length in the range of 5-10 cm, a gel pH range of 3-10, and a gel pH gradient in the direction of migration, where the total length of the gel is 1, the gel length up to pH 5 is a, the gel length at pH 5-7 is b, and the gel length at pH 7 or higher is c, where a is in the range of 0.15-0.3, b is in the range of 0.4-0.7, and c is in the range of 0.15-0.3. Specifically, isoelectric focusing was performed using IPG gel Immobiline Drystrip (pH 3-10NL) manufactured by GE Healthcare Biosciences, Inc. (hereinafter abbreviated as GE). The electrophoresis equipment used was IPGphor manufactured by GE. The upper limit of the current value of the electrophoresis device was set to 75 μA per gel, and the voltage program was as follows: (1) a constant voltage step was performed at 300 V constant voltage up to 750 Vhr (the current change width during the 30 minutes of electrophoresis before the end of this step was 5 μA), (2) the voltage was gradually increased to 1000 V over 300 Vhr, (3) the voltage was further gradually increased to 5000 V over 4500 Vhr, and (4) the first dimension isoelectric focusing was then performed at a constant voltage of 5000 V until the total Vhr reached 12000.

[0040] For the second-dimensional electrophoresis, polyacrylamide gel was used, in which the gel concentration at the base end of the electrophoresis direction was set to 3-6% and the gel concentration at the tip end of the electrophoresis direction was set higher than the gel concentration at the base end of the electrophoresis direction. Specifically, SDS-PAGE was performed using NuPAGE 4-12% Bris-Tris Gels IPG well mini 1mm manufactured by Life Technologies. The electrophoresis equipment was a Life Technologies product. The electrophoresis buffer used was 50 mM MOPS, 50 mM Tris base, 0.1% (w / v) SDS, and 1 mM EDTA were used, and electrophoresis was carried out at a constant voltage of 200 V for approximately 45 minutes.

[0041] As a result, when soybean proteins were subjected to two-dimensional electrophoresis under the above conditions, the following spots in the gel were found to specifically bind to IgE antibodies from patients with soybean allergies. Spot 1: Molecular weight 1-30 kDa, pI 3.0-7.0 Spot 2: Molecular weight 30-75 kDa, pI 4.0-8.5 Spot 3: Molecular weight 20-50 kDa, pI 7.0-12.0 Spot 4: Molecular weight 20-50 kDa, pI 6.0-10.0 Spot 5: Molecular weight 2-30 kDa, pI 4.0-9.0 Spot 6: Molecular weight 50-100 kDa, pI 5.0-10.0 Spot 7: Molecular weight 5-37 kDa, pI 3.0-7.0 Spot 8: Molecular weight 75-200 kDa, pI 4.0-6.0 Spot 1α: molecular weight 70-120 kDa, pI 2.5-6.5 Spot 2α: molecular weight 50-120 kDa, pI 2.5-6.5 Spot 4α: molecular weight 70-120 kDa, pI 3.5-7.5 Spot 5α: molecular weight 70-170 kDa, pI 3.5-7.5 Spot 6α: molecular weight 70-120 kDa, pI 3.5-7.5 Spot 7α: molecular weight 50-120 kDa, pI 3.5-7.5 Spot 8α: molecular weight 50-120 kDa, pI 3.5-8.5 Spot 9α: molecular weight 50-120 kDa, pI 3.5-8.5 Spot 10α: molecular weight 30-90 kDa, pI 3.5-7.5 Spot 11α: molecular weight 30-90 kDa, pI 3.5-8.5 Spot 12α: molecular weight 30-90 kDa, pI 3.5-8.5 Spot 13α: molecular weight 30-90 kDa, pI 3.5-7.5 Spot 14α: molecular weight 30-90 kDa, pI 2.5-6.5 Spots 15α and 16α: molecular weight 20-90 kDa, pI 3.5-7.5 Spot 17α: molecular weight 20-90 kDa, pI 3.5-7.5 Spot 18α: molecular weight 30-90 kDa, pI 3.5-8.5 Spot 19α: molecular weight 30-90 kDa, pI 3.5-8.5 Spot 25α: molecular weight 20-90 kDa, pI 3.5-7.5 Spot 26α: molecular weight 20-90 kDa, pI 3.5-7.5 Spot 27α: molecular weight 20-90 kDa, pI 3.5-7.5 Spot 28α: molecular weight 10-70 kDa, pI 3.5-7.5 Spots 29α, 30α, 31α, 32α, 33α: molecular weight 10-70 kDa, pI 3.5-8.5 Spot 34α: molecular weight 10-70 kDa, pI 5.5-10.5 Spot 35α: molecular weight 20-90 kDa, pI 5.5-10.5 Spots 36α and 37α: molecular weight 10-70 kDa, pI 3.5-8.5 Spot 38α: molecular weight 70-170 kDa, pI 5.5-10.5 Spot 39α: molecular weight 5-60 kDa, pI 5.5-10.5 Spots 40α, 56α: molecular weight 5-60 kDa, pI 5.5-10.5 Spot 41α: molecular weight 5-60 kDa, pI 5.5-10.5 Spots 42α and 43α: molecular weight 5-60 kDa, pI 5.5-10.5 Spot 45α: molecular weight 2-50 kDa, pI 3.5-7.5 Spot 46α: molecular weight 2-50 kDa, pI 3.5-7.5 Spot 47α: molecular weight 5-50 kDa, pI 3.5-7.5 Spot 48α: molecular weight 2-50 kDa, pI 3.5-8.5 Spot 50α: molecular weight 2-50 kDa, pI 2.5-6.5 Spot 51α: molecular weight 5-60 kDa, pI 2.5-6.5 Spot 52α: molecular weight 10-70 kDa, pI 3.5-7.5 Spot 53α: molecular weight 5-60 kDa, pI 3.5-8.5 Spot 54α: molecular weight 5-60 kDa, pI 3.5-8.5 Spot 55α: molecular weight 30-90 kDa, pI 5.5-10.5 antigen (1) Spot 1 antigen As a result of sequence identification by mass spectrometry for spot 1, the following amino acid sequence was detected. DQSSSYDDDEYSK (SEQ ID NO: 17) KSDQSSSYDDDEYSK (SEQ ID NO: 18) SDQSSSYDDDEYSK (SEQ ID NO: 19) SYDDDEYSK (SEQ ID NO: 20) In addition, for spot 1, the mass data obtained from the mass spectrometer was analyzed by comparing it with the protein data from Uniprot, and it was identified as Bowman-Birk type proteinase inhibitor D-II (amino acid sequence: SEQ ID NO: 2, base sequence encoding it: SEQ ID NO: 1).

[0042] Therefore, in the present application, the antigens of spot 1 are the following (1A-a) to (1A-e) and (1B) or (1C). (1A-a) SEQ ID NO: 2 in which one or several amino acids are deleted, substituted, inserted or added A protein containing an amino acid sequence. (1A-b) A sequence having an identity of 70% or more, preferably 75% or more, with the amino acid sequence shown in SEQ ID NO: 2. % or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more of the amino acid sequence. (1A-c) One or several nucleotides are deleted, substituted, inserted or added in SEQ ID NO:1 A protein comprising an amino acid sequence encoded by a base sequence. (1A-d) A sequence having an identity of 70% or more, preferably 75% or more, to the base sequence shown in SEQ ID NO: 1. or more, a protein comprising an amino acid sequence encoded by 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more of a base sequence. (1A-e) A nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 1 and a strand A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under homogenous conditions. (1B) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 17 to 20, preferably a protein comprising at least two, three, or all of the amino acid sequences. More preferably, a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 17 to 19, preferably a protein comprising at least two or all of the amino acid sequences. Here, the amino acid sequence shown in any of SEQ ID NOs: 17 to 20 may have one or several amino acids deleted, substituted, inserted, or added.

[0043] The above proteins (1A-a) to (1A-e) and (1B) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, etc.

[0044] The above proteins (1A-a) to (1A-e) and (1B) may be proteins that appear as spots having a molecular weight of 1 kDa to 30 kDa, preferably a molecular weight of 2 kDa to 25 kDa, more preferably a molecular weight of about 5 to 20 kDa, and an isoelectric point of 3.0 to 7.0, preferably 4.0 to 6.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the above section "Identification of antigen."

[0045] Preferably, the protein that is the antigen of spot 1 has activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 2 (Bowman-Birk type proteinase inhibitor D-II), or causes allergy to soybean.

[0046] (2) Spot 2 antigen As a result of sequence identification by mass spectrometry for spot 2, the following amino acid sequence was detected. ALRALRLEDLRIPTAYIK (SEQ ID NO:21) ALRLSGGDHVHAGTVVGK (SEQ ID NO: 22) LEGEREITLGFVDLLRDDFVEK (SEQ ID NO:23) LRLEDLRIPTAYIK (SEQ ID NO: 24) LTYYTPDYETK (SEQ ID NO: 25) TDGLTSLDRYK (SEQ ID NO: 26) TFQGPPHGIQVERD (SEQ ID NO: 27) TFQGPPHGIQVERDK (SEQ ID NO: 28) In addition, the mass data obtained from the mass spectrometer for spot 2 was analyzed by collating it with the protein data from Uniprot, and it was found to be ribulose bisphosphate carboxylase large chain (amino acid sequence: The sequence of the gene encoding this variant was identified as SEQ ID NO: 4 and the base sequence encoding it: SEQ ID NO: 3).

[0047] Therefore, in the present application, the antigen of spot 2 may be any of the following (2A-a) to (2A-e) and (2B). (2A-a) SEQ ID NO: 4 in which one or several amino acids are deleted, substituted, inserted or added A protein containing an amino acid sequence. (2A-b) A sequence having an identity of 70% or more, preferably 75% or more, to the amino acid sequence shown in SEQ ID NO: 4. % or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more of the amino acid sequence. (2A-c) One or several nucleotides are deleted, substituted, inserted or added in SEQ ID NO:3 A protein comprising an amino acid sequence encoded by a base sequence. (2A-d) A sequence having an identity of 70% or more, preferably 75% or more, to the base sequence shown in SEQ ID NO: 3. or more, a protein comprising an amino acid sequence encoded by 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more of a base sequence. (2A-e) A nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 3 and a strand A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under homogenous conditions. (2B) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 21 to 28, preferably at least 2, 3, 4, 5, 6, 7, or all of the amino acid sequences, wherein one or several amino acids may be deleted, substituted, inserted, or added in the amino acid sequence represented by any of SEQ ID NOs: 21 to 28.

[0048] The above proteins (2A-a) to (2A-e) and (2B) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, or the like.

[0049] The above proteins (2A-a) to (2A-e) and (2B) may be proteins that appear as spots having a molecular weight of about 30 kDa to 75 kDa, preferably a molecular weight of about 37 kDa to 70 kDa, more preferably a molecular weight of about 40 to 60 kDa, and an isoelectric point of about 4.0 to 8.5, preferably 5.5 to 7.5, in a gel when two-dimensional electrophoresis is performed under the conditions described in the above section "Identification of antigen."

[0050] Preferably, the protein that is the antigen of spot 2 has an activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 4 (ribulose bisphosphate carboxylase large chain). or cause an allergy to soy.

[0051] (3) Spot 3 antigen As a result of sequence identification by mass spectrometry for spot 3, the following amino acid sequence was detected. APHTEDAIRVTQSTANFFISEARK (SEQ ID NO:29) FVESGGARVIPLIYNESPENLNK (SEQ ID NO: 30) In addition, when the mass data obtained from the mass spectrometer for spot 3 was analyzed by comparing it with the protein data from Uniprot, it was identified as gamma-glutamyl hydrolase (amino acid sequence: SEQ ID NO: 6, base sequence encoding it: SEQ ID NO: 5).

[0052] Therefore, in the present application, the antigen of spot 3 may be any of the following (3A-a) to (3A-e) and (3B). (3A-a) SEQ ID NO: 6 in which one or several amino acids are deleted, substituted, inserted or added A protein containing an amino acid sequence. (3A-b) A sequence having an identity of 70% or more, preferably 75% or more, with the amino acid sequence shown in SEQ ID NO:6. % or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more of the amino acid sequence. (3A-c) One or several nucleotides are deleted, substituted, inserted or added in SEQ ID NO:5 A protein comprising an amino acid sequence encoded by the base sequence, or a glycosylated form thereof. (3A-d) A sequence having an identity of 70% or more, preferably 75% or more, to the base sequence shown in SEQ ID NO:5. or more, a protein comprising an amino acid sequence encoded by 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more of a base sequence. (3A-e) A nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO:5 and a strand A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under homogenous conditions. (3B) A protein comprising the amino acid sequence shown in SEQ ID NO: 29 or 30, preferably a protein comprising the amino acid sequence shown in SEQ ID NO: 29 and 30. In this regard, the amino acid sequence shown in SEQ ID NO: 29 and / or 30 may have one or several amino acids deleted, substituted, inserted or added.

[0053] The above proteins (3A-a) to (3A-e) and (3B) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, or the like.

[0054] The above proteins (3A-a) to (3A-e) and (3B) may be proteins that appear as spots having a molecular weight of about 20 kDa to 50 kDa, preferably a molecular weight of about 25 kDa to 40 kDa, more preferably a molecular weight of about 25 to 37 kDa, and an isoelectric point of about 7.0 to 12.0, preferably 8.0 to 10.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the above section "Identification of antigen."

[0055] Preferably, the protein that is the antigen of spot 3 has activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 6 (Gamma-glutamyl hydrolase), or causes allergy to soybean.

[0056] (4) Spot 4 antigen Spot 4 was subjected to sequence identification by mass spectrometry, and the following amino acid sequence was detected. IWDLESK (SEQ ID NO:31) SIVEDLK (SEQ ID NO:32) In addition, when the mass data obtained from the mass spectrometer for spot 4 was analyzed by comparing it with the protein data from Uniprot, it was identified as Guanine nucleotide-binding protein subunit beta-like protein (amino acid sequence: SEQ ID NO: 8, base sequence encoding it: SEQ ID NO: 7).

[0057] Therefore, in the present application, the antigen of spot 4 may be any of the following (4A-a) to (4A-e) and (4B). (4A-a) SEQ ID NO: 8 in which one or several amino acids are deleted, substituted, inserted or added A protein containing an amino acid sequence. (4A-b) A sequence having an identity of 70% or more, preferably 75% or more, to the amino acid sequence shown in SEQ ID NO: 8. % or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more of the amino acid sequence. (4A-c) One or several nucleotides are deleted, substituted, inserted or added in SEQ ID NO: 7 A protein comprising an amino acid sequence encoded by a base sequence. (4A-d) A sequence having an identity of 70% or more, preferably 75% or more, to the base sequence shown in SEQ ID NO: 7. or more, a protein comprising an amino acid sequence encoded by 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more of a base sequence. (4A-e) A nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 7 and a strand A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under homogenous conditions. (4B) A protein comprising the amino acid sequence shown in SEQ ID NO: 31 or 32, preferably a protein comprising the amino acid sequence shown in SEQ ID NO: 31 and 32. In this regard, the amino acid sequence shown in SEQ ID NO: 31 and / or 32 may have one or several amino acids deleted, substituted, inserted or added.

[0058] The above proteins (4A-a) to (4A-e) and (4B) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, or the like.

[0059] The above proteins (4A-a) to (4A-e) and (4B) may be proteins that appear as spots having a molecular weight of about 20 kDa to 50 kDa, preferably a molecular weight of about 25 kDa to 40 kDa, more preferably a molecular weight of about 25 to 37 kDa, and an isoelectric point of about 6.0 to 10.0, preferably 7.0 to 9.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the above section "Identification of antigen."

[0060] Preferably, the protein that is the antigen of spot 4 has activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 8 (Guanine nucleotide-binding protein subunit beta-like protein), or causes allergy to soybean.

[0061] (5) Spot 5 antigen Spot 5 was subjected to sequence identification by mass spectrometry, and the following amino acid sequence was detected. QELDERARQGETVVPGGTGGK (SEQ ID NO: 33) SGEERAQEEAIDIDESK (SEQ ID NO: 34) In addition, when the mass data obtained from the mass spectrometer for spot 5 was analyzed by comparing it with the protein data from Uniprot, it was identified as Protein SLE3 (amino acid sequence: SEQ ID NO: 10, the base sequence encoding it: SEQ ID NO: 9).

[0062] Therefore, in the present application, the antigen of spot 5 may be any of the following (5A-a) to (5A-e) and (5B). (5A-a) SEQ ID NO: 10 in which one or several amino acids are deleted, substituted, inserted or added A protein comprising the selected amino acid sequence. (5A-b) A sequence having an identity of 70% or more, preferably 70%, to the amino acid sequence shown in SEQ ID NO: 10. A protein that contains 5% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more of the amino acid sequence. (5A-c) One or several nucleotides are deleted, substituted, inserted or added in SEQ ID NO:9 A protein comprising an amino acid sequence encoded by a base sequence. (5A-d) A sequence having an identity of 70% or more, preferably 75% or more, to the base sequence shown in SEQ ID NO: 9. or more, a protein comprising an amino acid sequence encoded by 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more of a base sequence. (5A-e) A nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 9 and a strand A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under homogenous conditions. (5B) A protein comprising the amino acid sequence shown in SEQ ID NO: 33 or 34, preferably a protein comprising the amino acid sequence shown in SEQ ID NO: 33 and 34. In this regard, the amino acid sequence shown in SEQ ID NO: 33 and / or 34 may have one or several amino acids deleted, substituted, inserted or added.

[0063] The above proteins (5A-a) to (5A-e) and (5B) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, etc.

[0064] The above proteins (5A-a) to (5A-e) and (5B) may be proteins that appear as spots having a molecular weight of 2 kDa to 30 kDa, preferably a molecular weight of 3 kDa to 25 kDa, more preferably a molecular weight of about 5 to 20 kDa, and an isoelectric point of 4.0 to 9.0, preferably 5.0 to 7.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the above section "Identification of antigen."

[0065] Preferably, the protein that is the antigen of spot 5 has activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 10 (Protein SLE3), or causes allergy to soybean.

[0066] (6) Spot 6 antigen Spot 6 was subjected to sequence identification by mass spectrometry, and the following amino acid sequence was detected. AAAHVVEGAAGYAGHK (SEQ ID NO: 35) AKDYTLQAAEK (SEQ ID NO: 36) ARETHELGAHFESLADK (SEQ ID NO: 37) ATAVGWAAAHFSAEK (SEQ ID NO:38) DTPQGSIEALQAGERVK (SEQ ID NO: 39) DYTLQAAEK (SEQ ID NO: 40) EEAQRELEAK (SEQ ID NO:41) EGTGSIVFTAIGETVSSAGEK (SEQ ID NO: 42) ERGRESGGQVVAEK (SEQ ID NO: 43) GLAASAGETAK (SEQ ID NO: 44) GQQGYAVTK (SEQ ID NO:45) KPSQPQEAEERPSEGIGETVRQYAQK (SEQ ID NO: 46) PAEIVQER (SEQ ID NO: 47) PQGSIEALQAGERVK (SEQ ID NO:48) QAASETLNTTTQTAQEK (SEQ ID NO:49) SAGGTTASYVGEK (SEQ ID NO:50) SLTSIGEK (SEQ ID NO:51) TPQGSIEALQAGERVK (SEQ ID NO:52) VTDHAAANVVGNK (SEQ ID NO:53) In addition, when the mass data obtained from the mass spectrometer for spot 6 was analyzed by comparing it with the protein data from Uniprot, it was identified as seed biotin-containing protein SBP65 (amino acid sequence: SEQ ID NO: 12, base sequence encoding it: SEQ ID NO: 11).

[0067] Therefore, in the present application, the antigen of spot 6 may be any of the following (6A-a) to (6A-e) and (6B). (6A-a) SEQ ID NO: 12 in which one or several amino acids are deleted, substituted, inserted or added A protein comprising the selected amino acid sequence. (6A-b) A sequence having an identity of 70% or more, preferably 70%, to the amino acid sequence shown in SEQ ID NO: 12. A protein that contains 5% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more of the amino acid sequence. (6A-c) SEQ ID NO: 11 in which one or several nucleotides are deleted, substituted, inserted or added A protein comprising an amino acid sequence encoded by the added base sequence. (6A-d) 70% or more, preferably 75% identity with the base sequence shown in SEQ ID NO: 11 A protein comprising an amino acid sequence encoded by 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more of a base sequence. (6A-e) A nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 11 and a sequence A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions. (6B) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 35 to 53, preferably at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, or all of the amino acid sequences. More preferably, a protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 35 to 39, 41 to 44, 46, 48 to 50, 52, and 53, preferably at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or all of the amino acid sequences. Here, one or several amino acids may be deleted, substituted, inserted, or added in the amino acid sequence represented by any of SEQ ID NOs: 35 to 53.

[0068] The above proteins (6A-a) to (6A-e) and (6B) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, etc.

[0069] The above proteins (6A-a) to (6A-e) and (6B) may be proteins that appear as spots having a molecular weight of about 50 kDa to 100 kDa, preferably a molecular weight of about 55 kDa to 90 kDa, more preferably a molecular weight of about 60 to 75 kDa, and an isoelectric point of about 5.0 to 10.0, preferably 6.0 to 8.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the above section "Identification of antigen."

[0070] Preferably, the protein that is the antigen of spot 6 has activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 12 (Seed biotin-containing protein SBP65), or causes allergy to soybean.

[0071] (7) Spot 7 antigen Spot 7 was subjected to sequence identification by mass spectrometry, and the following amino acid sequence was detected. AASNAPAPLAGVIFEPFQELKK (SEQ ID NO:54) ASNAPAPLAGVIFEPFQELKK (SEQ ID NO:55) FFKESSEEEREHAEQLIK (SEQ ID NO:56) GHGVWHFDQK (SEQ ID NO:57) IAEYVAQLRLVGK (SEQ ID NO:58) KIAEYVAQLRLVGK (SEQ ID NO:59) LLHDEDHV (SEQ ID NO: 60) SNAPAPLAGVIFEPFQELKK (SEQ ID NO:61) In addition, the mass data obtained from the mass spectrometer for spot 7 was analyzed by collating it with the protein data from Uniprot, and it was found to be ferritin-2 (amino acid The sequence was identified as SEQ ID NO:14, and the base sequence encoding it: SEQ ID NO:13).

[0072] Therefore, in the present application, the antigen of spot 7 may be any of the following (7A-a) to (7A-e) and (7B). (7A-a) SEQ ID NO: 14 in which one or several amino acids are deleted, substituted, inserted or added A protein comprising the selected amino acid sequence. (7A-b) A sequence having an identity of 70% or more, preferably 70%, to the amino acid sequence shown in SEQ ID NO: 14. A protein that contains 5% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more of the amino acid sequence. (7A-c) SEQ ID NO: 13 in which one or several nucleotides are deleted, substituted, inserted or added A protein comprising an amino acid sequence encoded by the added base sequence. (7A-d) 70% or more, preferably 75% identity to the base sequence shown in SEQ ID NO: 13 A protein comprising an amino acid sequence encoded by 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more of a base sequence. (7A-e) A nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 13. A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions. (7B) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 54 to 61, preferably a protein comprising at least 2, 3, 4, 5, 6, 7, or all of the amino acid sequences. More preferably, a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 54 to 59 and 61, preferably a protein comprising at least 2, 3, 4, 5, 6, or all of the amino acid sequences. Here, the amino acid sequence shown in any of SEQ ID NOs: 54 to 61 may have one or several amino acids deleted, substituted, inserted, or added.

[0073] The above proteins (7A-a) to (7A-e) and (7B) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, etc.

[0074] The above proteins (7A-a) to (7A-e) and (7B) may be proteins that appear as spots having a molecular weight of about 5 kDa to 37 kDa, preferably a molecular weight of about 10 kDa to 30 kDa, more preferably a molecular weight of about 15 to 25 kDa, and an isoelectric point of 3.0 to 7.0, preferably 4.0 to 6.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the above section "Identification of antigen."

[0075] Preferably, the protein that is the antigen of spot 7 has activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 14 (Ferritin-2), or causes allergy to soybean.

[0076] (8) Spot 8 antigen Spot 8 was subjected to sequence identification by mass spectrometry, and the following amino acid sequence was detected. AGESESNLRK (SEQ ID NO:62) GESEANVREIFDK (SEQ ID NO:63) GILLYGPPGSGK (SEQ ID NO:64) LAGESESNLRK (SEQ ID NO:65) LSDDVDLERIAK (SEQ ID NO:66) RELQETVQYPVEHPEK (SEQ ID NO:67) TVFIIGATNRPDIID (SEQ ID NO: 68) In addition, when the mass data obtained from the mass spectrometer for spot 8 was analyzed by comparing it with the protein data from Uniprot, it was identified as cell division cycle protein 48 homolog (amino acid sequence: SEQ ID NO: 16, base sequence encoding it: SEQ ID NO: 15).

[0077] Therefore, in the present application, the antigen of spot 8 may be any of the following (8A-a) to (8A-e) and (8B). (8A-a) SEQ ID NO: 16 in which one or several amino acids are deleted, substituted, inserted or added A protein comprising the selected amino acid sequence. (8A-b) A sequence having an identity of 70% or more, preferably 70%, to the amino acid sequence shown in SEQ ID NO: 16. A protein that contains 5% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more of the amino acid sequence. (8A-c) SEQ ID NO: 15 in which one or several nucleotides are deleted, substituted, inserted or added A protein comprising an amino acid sequence encoded by the added base sequence. (8A-d) 70% or more, preferably 75% identity to the base sequence shown in SEQ ID NO: 15 A protein comprising an amino acid sequence encoded by 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more of a base sequence. (8A-e) A nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 15. A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions. (8B) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 62 to 68, preferably at least 2, 3, 4, 5, 6, or all of the amino acid sequences, wherein the amino acid sequence represented by any of SEQ ID NOs: 62 to 68 may have one or several amino acids deleted, substituted, inserted, or added.

[0078] The above proteins (8A-a) to (8A-e) and (8B) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, etc.

[0079] The above proteins (8A-a) to (8A-e) and (8B) may be proteins that appear as spots having a molecular weight of about 75 kDa to 200 kDa, preferably a molecular weight of about 100 kDa to 170 kDa, more preferably a molecular weight of about 100 to 150 kDa, and an isoelectric point of 3.0 to 7.0, preferably 4.0 to 6.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the above section "Identification of antigen."

[0080] Preferably, the protein that is the antigen of spot 8 has activity equivalent to the protein having the amino acid sequence of SEQ ID NO: 16 (Cell division cycle protein 48 homolog), or causes allergy to soybean.

[0081] (1α) Spot 1α antigen As a result of sequence identification by mass spectrometry for spot 1α, the following amino acid sequence was detected. APQDLYESYYNANK (SEQ ID NO:71) AVLFVPPK (SEQ ID NO:72) DFASRIYDSVK (SEQ ID NO:73) DIFLRELISNASDALDK (SEQ ID NO:74) DIFYITGTSK (SEQ ID NO:75) DNVDDVK (SEQ ID NO: 76) EDSKPETDAVNDDNDVK (SEQ ID NO: 77) EEAGEYLEESK (SEQ ID NO:78) EQLENSPFLERLK (SEQ ID NO:79) EVLGEGDNTK (SEQ ID NO: 80) EVTEEEYTK (SEQ ID NO:81) FYHSLAK (SEQ ID NO:82) IGAVPHGLSTDSDVVK (SEQ ID NO: 83) LGIIEDATNRNRLAK (SEQ ID NO: 84) NPEDEGVK (SEQ ID NO: 85) SGTSAFVEK (SEQ ID NO: 86) TSLDISPEATVEEEDDTEVEAESDAK (SEQ ID NO: 87) APQDLYESYYNANK (SEQ ID NO: 90) AVLFVPPK (SEQ ID NO: 91) DFASRIYDSVK (SEQ ID NO: 92) DIFLRELISNASDALDK (SEQ ID NO:93) DIFYITGTSK (SEQ ID NO:94) DNVDDVK (SEQ ID NO: 95) DVLGEGDNTK (SEQ ID NO: 96) EEAGEYLQESK (SEQ ID NO: 97) EQLENSPFLERLK (SEQ ID NO:98) EVTEEEYTK (SEQ ID NO: 99) FYHSLAK (SEQ ID NO: 100) IGAVPHGLSTDSDVVK (SEQ ID NO: 101) LGIIEDATNRNRLAK (SEQ ID NO: 102) NPEDEGVK (SEQ ID NO: 103) SGTSAFVEK (SEQ ID NO: 104) In addition, when the mass data obtained from a mass spectrometer for spot 1α was analyzed by comparing it with NCBI protein data, endoplasmin homolog isoform X2 (amino acid sequence: SEQ ID NO: 70, nucleotide sequence encoding same: SEQ ID NO: 69) was identified as a protein containing any of SEQ ID NOs: 71 to 87, and endoplasmin homolog isoform X2 (amino acid sequence: SEQ ID NO: 89, nucleotide sequence encoding same: SEQ ID NO: 88) was identified as a protein containing any of SEQ ID NOs: 90 to 104.

[0082] Therefore, in the present application, the antigen of spot 1α may be any of the proteins selected from the group consisting of (1αA1-a) to (1αA1-e), (1αB1), (1αA2-a) to (1αA2-e), and (1αB2). (1αA1-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 70. (1αA1-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 70. (1αA1-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO:69 have been deleted, substituted, inserted or added. (1αA1-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 69. (1αA1-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 69. (1αB1) At least one amino acid sequence selected from the group consisting of SEQ ID NOs: 71 to 87 A protein comprising one of the amino acid sequences, preferably a protein comprising at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, or all of the amino acid sequences, wherein the amino acid sequence shown in any of SEQ ID NOs: 71 to 87 may have one or several amino acids deleted, substituted, inserted, or added. (1αA2-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO:89. (1αA2-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 89. (1αA2-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 88 have been deleted, substituted, inserted or added. (1αA2-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 88. (1αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 88. (1αB2) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 90 to 104, preferably at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or all of the amino acid sequences, wherein one or several amino acids may be deleted, substituted, inserted, or added in the amino acid sequence represented by any of SEQ ID NOs: 90 to 104.

[0083] Proteins selected from the group consisting of (1αA1-a) to (1αA1-e), (1αB1), (1αA2-a) to (1αA2-e), and (1αB2) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, etc.

[0084] The protein selected from the group consisting of (1αA1-a) to (1αA1-e), (1αB1), (1αA2-a) to (1αA2-e), and (1αB2) may be a protein that appears as a spot with a molecular weight of about 70 to 120 kDa, preferably a molecular weight of about 80 to 110 kDa, and an isoelectric point of 2.5 to 6.5, preferably 3.0 to 6.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above.

[0085] Preferably, the protein that is an antigen of spot 1α has activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 70 or 89 (endoplasmin homolog isoform X2), or causes allergy to soybean.

[0086] (2α) Spot 2α antigen As a result of sequence identification by mass spectrometry for spot 2α, the following amino acid sequence was detected. ADLVNNLGTIARSGTK (SEQ ID NO: 107) AVENSPFLEK (SEQ ID NO: 108) EDQLEYLEERRLK (SEQ ID NO: 109) EEYAAFYK (SEQ ID NO: 110) EGQNDIYYITGESK (SEQ ID NO: 111) EIFLRELISNASDALDK (SEQ ID NO: 112) FYEAFSK (sequence number 113) HFSVEGQLEFK (SEQ ID NO: 114) IRFESLTDK (SEQ ID NO: 115) LAELLRYHSTK (SEQ ID NO: 116) LDESEDEK (sequence number 117) LGIHEDSQNK (SEQ ID NO: 118) RAPFDLFDTRK (SEQ ID NO: 119) VEDVDEDK (SEQ ID NO: 120) ADLVNNLGTIARSGTK (SEQ ID NO: 123) AVENSPFLEK (SEQ ID NO: 124) EDQLEYLEERRLK (SEQ ID NO: 125) EEYSAFYK (SEQ ID NO: 126) EGQSDIYYITGESK (SEQ ID NO: 127) EIFLRELISNASDALDK (SEQ ID NO: 128) FYEAFSK (sequence number 129) HFSVEGQLEFK (SEQ ID NO: 130) IAELLRYHSTK (SEQ ID NO: 131) IRFESLTDK (SEQ ID NO: 132) LDESEDEK (SEQ ID NO: 133) LGIHEDSQNK (SEQ ID NO: 134) RAPFDLFDTRK (SEQ ID NO: 135) SGDELTSLK (SEQ ID NO: 136) VEDVDEEK (SEQ ID NO: 137) ADLVNNLGTIARSGTK (SEQ ID NO: 140) AVENSPFLEK (SEQ ID NO: 141) EDQLEYLEERRLK (SEQ ID NO: 142) EEYAAFYK (SEQ ID NO: 143) EGQNDIYYITGESK (SEQ ID NO: 144) EIFLRELISNASDALDK (SEQ ID NO: 145) FYEAFSK (sequence number 146) HFSVEGQLEFK (SEQ ID NO: 147) IRFESLTDK (SEQ ID NO: 148) LAELLRYHSTK (SEQ ID NO: 149) LDESEDEK (sequence number 150) LGIHEDSQNK (SEQ ID NO: 151) RAPFDLFDTK (SEQ ID NO: 152) VEDVDEDK (SEQ ID NO: 153) In addition, the mass data obtained from the mass spectrometer for Spot 2α was analyzed by collating it with the protein data of NCBI, and the proteins containing any of SEQ ID NOs: 107 to 120 were identified as heat shock cognate protein 80 (amino acid sequence: SEQ ID NO: 106, base sequence encoding same: SEQ ID NO: 105), As a protein containing any one of SEQ ID NOs: 123 to 137, heat shock protein 90-1 (amino acid sequence: SEQ ID NO: 122, As a protein comprising any one of the base sequences represented by SEQ ID NO:121 and SEQ ID NOs:140 to 153, hypothetical protein GLYMA_02G302500 was identified.

[0087] Therefore, in the present application, the antigen of spot 2α may be any of the proteins selected from the group consisting of (2αA1-a) to (2αA1-e), (2αB1), (2αA2-a) to (2αA2-e), (2αB2), (2αA3-a) to (2αA3-e), and (2αB3). (2αA1-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 106. (2αA1-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 106. (2αA1-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 105 have been deleted, substituted, inserted or added. (2αA1-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 105. (2αA1-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 105. (2αB1) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 107 to 120, preferably at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, or all of said amino acid sequences, wherein one or several amino acids may be deleted, substituted, inserted, or added in any of the amino acid sequences shown in SEQ ID NOs: 107 to 120. (2αA2-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 122. (2αA2-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 122. (2αA2-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 121 have been deleted, substituted, inserted or added. (2αA2-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 121. (2αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO:121. (2αB2) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 123 to 137, preferably at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or all of the amino acid sequences, wherein one or several amino acids may be deleted, substituted, inserted, or added in the amino acid sequence represented by any of SEQ ID NOs: 123 to 137. (2αA3-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 139. (2αA3-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 139. (2αA3-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 138 have been deleted, substituted, inserted or added. (2αA3-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 138. (2αA3-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 138. (2αB3) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 140 to 153, preferably a protein comprising at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, or all of the amino acid sequences. In the amino acid sequence shown in any of SEQ ID NOs: 140 to 153, one or several amino acids may be deleted, substituted, inserted or added.

[0088] The proteins selected from the group consisting of (2αA1-a) to (2αA1-e), (2αB1), (2αA2-a) to (2αA2-e), (2αB2), (2αA3-a) to (2αA3-e), and (2αB3) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, etc.

[0089] The protein selected from the group consisting of (2αA1-a) to (2αA1-e), (2αB1), (2αA2-a) to (2αA2-e), (2αB2), (2αA3-a) to (2αA3-e), and (2αB3) may be a protein that appears as a spot with a molecular weight of 50 to 120 kDa, preferably a molecular weight of about 60 to 110 kDa, and an isoelectric point of 2.5 to 6.5, preferably 3.0 to 6.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the above section ``Identification of antigen.''

[0090] Preferably, the protein that is the antigen of spot 2α is a protein having the amino acid sequence of SEQ ID NO: 106 (heat shock cognate protein 80), a protein having the amino acid sequence of SEQ ID NO: 122, Protein having the sequence (heat shock protein 90-1) or amino acid sequence of SEQ ID NO: 139 It has activity equivalent to that of the protein (hypothetical protein GLYMA_02G302500), or causes allergy to soybeans.

[0091] (4α) Spot 4α antigen As a result of sequence identification by mass spectrometry for spot 4α, the following amino acid sequence was detected. AGGALSIAFVSK (SEQ ID NO: 156) DGLIVSIGK (SEQ ID NO: 157) In addition, the mass data obtained from the mass spectrometer for spot 4α was analyzed by collating it with the protein data of NCBI, and it was identified as embryo-specific urease isoform X1 (amino acid sequence: SEQ ID NO: 155, the base sequence encoding it) as a protein containing either SEQ ID NO: 156 or 157. :SEQ ID NO: 154), was identified.

[0092] Therefore, in the present application, the antigen of spot 4α may be any protein selected from the group consisting of (4αA-a) to (4αA-e) and (4αB) below. (4αA-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 155. (4αA-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 155. (4αA-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 154 have been deleted, substituted, inserted or added. (4αA-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 154. (4αA-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 154. (4αB) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 156 and 157, preferably a protein comprising both of said amino acid sequences. In the amino acid sequence shown in either SEQ ID NO: 156 or 157, one or several amino acids may be deleted, substituted, inserted or added.

[0093] Proteins selected from the group consisting of (4αA-a) to (4αA-e) and (4αB) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, etc.

[0094] The protein selected from the group consisting of (4αA-a) to (4αA-e) and (4αB) above may be a protein that appears as a spot with a molecular weight of about 70 to 120 kDa, preferably a molecular weight of about 80 to 110 kDa, and an isoelectric point of 3.5 to 7.5, preferably 4.0 to 7.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above.

[0095] Preferably, the protein that is an antigen of spot 4α has an activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 155 (embryo-specific urease isoform X1), or causes allergies to soybeans.

[0096] (5α) Spot 5α antigen As a result of sequence identification by mass spectrometry for spot 5α, the following amino acid sequence was detected. AFATYVQAK (SEQ ID NO: 160) QQEATTSLSSFTPDASSIASSIK (sequence number 161) In addition, when the mass data obtained from a mass spectrometer for spot 5α was analyzed by comparing it with NCBI protein data, a protein containing either sequence number 160 or 161 was identified as alpha-1,4 glucan phosphorylase L isozyme, chloroplastic / amyloplastic (amino acid sequence: sequence number 159, base sequence encoding it: sequence number 158).

[0097] Therefore, in the present application, the antigen of spot 5α may be any protein selected from the group consisting of (5αA-a) to (5αA-e) and (5αB) below. (5αA-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 159. (5αA-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 159. (5αA-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides in SEQ ID NO: 158 have been deleted, substituted, inserted or added. (5αA-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 158. (5αA-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 158. (5αB) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NO: 160 and 161, preferably comprising both of said amino acid sequences, wherein the amino acid sequence represented by either SEQ ID NO: 160 or 161 may have one or several amino acids deleted, substituted, inserted or added.

[0098] A group selected from the group consisting of (5αA-a) to (5αA-e) and (5αB). Proteins also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, or the like.

[0099] The protein selected from the group consisting of (5αA-a) to (5αA-e) and (5αB) above may be a protein that appears as a spot with a molecular weight of about 70 to 170 kDa, preferably a molecular weight of about 80 to 160 kDa, and an isoelectric point of 3.5 to 7.5, preferably 4.0 to 7.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above.

[0100] Preferably, the protein that is an antigen of spot 5α has activity equivalent to the protein having the amino acid sequence of SEQ ID NO: 159 (alpha-1,4 glucan phosphorylase L isozyme, chloroplastic / amyloplastic) or causes allergy to soybean.

[0101] (6α) Spot 6α antigen As a result of sequence identification by mass spectrometry for spot 6α, the following amino acid sequence was detected. FLNGEVGPK (SEQ ID NO: 164) LSVFDAAEK (SEQ ID NO: 165) LSVFDVAEK (SEQ ID NO: 166) TSLAPGSGVVTK (SEQ ID NO: 167) FLNGEVGPK (SEQ ID NO: 170) LSVFDVAEK (SEQ ID NO: 171) TSLAPGSGVVTK (SEQ ID NO: 172) In addition, when the mass data obtained from the mass spectrometer for spot 6α was analyzed by collating it with the protein data of NCBI, it was identified as a protein containing any one of SEQ ID NOs: 164 to 167, which was aconitate hydratase 1 (amino acid sequence: Aconitate hydratase 1 (amino acid sequence: SEQ ID NO: 169, base sequence encoding same: SEQ ID NO: 168) was identified as a protein containing any one of SEQ ID NO: 163, base sequence encoding same: SEQ ID NO: 162, and SEQ ID NO: 170 to 172.

[0102] Therefore, in the present application, the antigen of spot 6α may be any of the proteins selected from the group consisting of (6αA1-a) to (6αA1-e), (6αB1), (6αA2-a) to (6αA2-e), and (6αB2). (6αA1-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 163. (6αA1-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 163. (6αA1-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 162 have been deleted, substituted, inserted or added. (6αA1-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 162. (6αA1-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 162. (6αB1) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 164 to 167, preferably at least two, three, or all of the amino acid sequences. In this case, the amino acid sequence shown in any of SEQ ID NOs: 164 to 167 has one or several amino acids deleted, substituted, inserted, or added. This is also fine. (6αA2-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 169. (6αA2-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 169. (6αA2-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 168 have been deleted, substituted, inserted or added. (6αA2-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 168. (6αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 168. (6αB2) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 170 to 172, preferably at least two or all of the amino acid sequences, wherein the amino acid sequence shown in any of SEQ ID NOs: 170 to 172 may have one or several amino acids deleted, substituted, inserted or added.

[0103] The proteins selected from the group consisting of (6αA1-a) to (6αA1-e), (6αB1), (6αA2-a) to (6αA2-e), and (6αB2) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, etc.

[0104] The protein selected from the group consisting of (6αA1-a) to (6αA1-e), (6αB1), (6αA2-a) to (6αA2-e), and (6αB2) may be a protein that appears as a spot with a molecular weight of about 70 to 120 kDa, preferably a molecular weight of about 80 to 110 kDa, and an isoelectric point of 3.5 to 7.5, preferably 4.0 to 7.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above.

[0105] Preferably, the protein that is the antigen of spot 6α has an activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 163 or 169 (aconitate hydratase 1); causes allergies to soybeans.

[0106] (7α) Spot 7α antigen As a result of sequence identification by mass spectrometry for spot 7α, the following amino acid sequence was detected. AGITVIQIDEAALREGLPLRK (SEQ ID NO: 175) ALGVDTVPVLVGPVTYLLLSKPAK (SEQ ID NO: 176) DEAFFSGNAAALASRK (SEQ ID NO: 177) DEVEDLEK (SEQ ID NO: 178) ISEEEYVK (SEQ ID NO: 179) IVEVNALAK (SEQ ID NO: 180) LIRNELAK (SEQ ID NO: 181) LLSVFREGVK (SEQ ID NO: 182) LVVSTSSSLLHTAVDLVNETK (SEQ ID NO: 183) SFSLLSLLPK (SEQ ID NO: 184) SSPRVTNEAVQK (SEQ ID NO: 185) TLTSLNGVTAYGFDLVRGTNTLDLIK (SEQ ID NO: 186) YGAGIGPGVYDIHSPRIPPTEEIADRINK (SEQ ID NO: 187) In addition, for spot 7α, the mass data obtained from the mass spectrometer was analyzed by collating it with the protein data of NCBI, and it was found to be a protein containing any one of SEQ ID NOs: 175 to 187, which is methionine synthase (amino acid sequence: SEQ ID NO: 174, and the base sequence encoding it: SEQ ID NO: 173), was identified.

[0107] Therefore, in the present application, the antigen of spot 7α may be any protein selected from the group consisting of (7αA-a) to (7αA-e) and (7αB) below. (7αA-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 174. (7αA-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 174. (7αA-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 173 have been deleted, substituted, inserted or added. (7αA-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 173. (7αA-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 173. (7αB) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 175 to 187, preferably at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, or all of the amino acid sequences, wherein one or several amino acids may be deleted, substituted, inserted, or added in the amino acid sequence shown in any of SEQ ID NOs: 175 to 187.

[0108] Proteins selected from the group consisting of (7αA-a) to (7αA-e) and (7αB) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, etc.

[0109] The protein selected from the group consisting of (7αA-a) to (7αA-e) and (7αB) above may be a protein that appears as a spot with a molecular weight of about 50 to 120 kDa, preferably a molecular weight of about 60 to 110 kDa, and an isoelectric point of 3.5 to 7.5, preferably 4.0 to 7.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above.

[0110] Preferably, the protein that is an antigen of spot 7α has an activity equivalent to that of a protein having the amino acid sequence of SEQ ID NO: 174 (methionine synthase), or has an activity against soybean. It can cause allergies.

[0111] (8α) Spot 8α antigen As a result of sequence identification by mass spectrometry for spot 8α, the following amino acid sequence was detected. AIGIDRFGASAPAGRIYK (SEQ ID NO: 190) ALPTYTPESPADATRNLSQTNLNALAK (SEQ ID NO: 191) ANSYSVHGSALGAK ​​(sequence number 192) ATADAALVEK (SEQ ID NO: 193) DKPTLIK (SEQ ID NO: 194) GGYTISDNSTGNKPDVILIGTGSELEIAAK (SEQ ID NO: 195) LPQLPGTSIEGVEK (SEQ ID NO: 196) NGNNGYDDIRAAIK (SEQ ID NO: 197) SVNTIRFLAIDAVEK (SEQ ID NO: 198) VTTTIGYGSPNK (SEQ ID NO: 199) In addition, when the mass data obtained from a mass spectrometer for spot 8α was analyzed by comparing it with NCBI protein data, transketolase, chloroplastic (amino acid sequence: SEQ ID NO: 189, base sequence encoding it: SEQ ID NO: 188) was identified as a protein containing any of SEQ ID NOs: 190 to 199.

[0112] Therefore, in the present application, the antigen of spot 8α may be any protein selected from the group consisting of (8αA-a) to (8αA-e) and (8αB) below. (8αA-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 189. (8αA-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 189. (8αA-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 188 have been deleted, substituted, inserted or added. (8αA-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 188. (8αA-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 188. (8αB) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 190 to 199, preferably at least 2, 3, 4, 5, 6, 7, 8, 9, or all of the amino acid sequences, wherein one or several amino acids may be deleted, substituted, inserted, or added in the amino acid sequence represented by any of SEQ ID NOs: 190 to 199.

[0113] Proteins selected from the group consisting of (8αA-a) to (8αA-e) and (8αB) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, etc.

[0114] The protein selected from the group consisting of (8αA-a) to (8αA-e) and (8αB) above may be a protein that appears as a spot with a molecular weight of about 50 to 120 kDa, preferably a molecular weight of about 60 to 110 kDa, and an isoelectric point of 3.5 to 8.5, preferably 4.0 to 8.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above.

[0115] Preferably, the protein that is an antigen of spot 8α has activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 189 (transketolase, chloroplastic), or causes allergy to soybean.

[0116] (9α) Spot 9α antigen As a result of sequence identification by mass spectrometry for spot 9α, the following amino acid sequence was detected. DLSSEEANQYLK (sequence number 202) In addition, the mass data obtained from the mass spectrometer for spot 9α was uploaded to the NCBI database. Analysis of the protein data revealed that the protein containing SEQ ID NO:202 was lysine-tRNA ligase, cytoplasmic-like isoform X2 (amino acid sequence: SEQ ID NO:201, coding for it). The base sequence corresponding to this gene (SEQ ID NO: 200) was identified.

[0117] Therefore, in the present application, the antigen of spot 9α may be any protein selected from the group consisting of (9αA-a) to (9αA-e) and (9αB) below. (9αA-a) A protein having an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 201. (9αA-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 201. (9αA-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides in SEQ ID NO: 200 have been deleted, substituted, inserted or added. (9αA-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 200. (9αA-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 200. (9αB) A protein comprising the amino acid sequence shown in SEQ ID NO: 202. In this case, the amino acid sequence shown in SEQ ID NO: 202 may have one or several amino acids deleted, substituted, inserted or added.

[0118] Proteins selected from the group consisting of (9αA-a) to (9αA-e) and (9αB) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, etc.

[0119] The protein selected from the group consisting of (9αA-a) to (9αA-e) and (9αB) above may be a protein that appears as a spot with a molecular weight of about 50 to 120 kDa, preferably a molecular weight of about 60 to 110 kDa, and an isoelectric point of 3.5 to 8.5, preferably 4.0 to 8.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above.

[0120] Preferably, the protein that is the antigen of spot 9α has an activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 201 (lysine-tRNA ligase, cytoplasmic-like isoform X2). It may have adverse effects on the body or may cause allergies to soybeans.

[0121] (10α) Spot 10α antigen As a result of sequence identification by mass spectrometry for spot 10α, the following amino acid sequence was detected. AAVEEGIVPGGGVALLYASSELDK (SEQ ID NO: 205) DRVTDALNATK (SEQ ID NO: 206) EGVITISDGK (SEQ ID NO: 207) GRNVVIEQSFGAPK (SEQ ID NO: 208) GVEELADAVK (sequence number 209) IGGASEAEVGEK (SEQ ID NO: 210) IGVQIIQNALK (SEQ ID NO: 211) ISSINAIVK (SEQ ID NO: 212) LLEQNDPDLGYDAAK (SEQ ID NO: 213) LQTANFDQK (SEQ ID NO: 214) LSGGVAVLK (SEQ ID NO: 215) NIGASLVK (sequence number 216) TGIIDPLK (SEQ ID NO: 217) TPVHTIASNAGVEGAVVVGK (SEQ ID NO: 218) AGIIDPLK (sequence number 221) DRVTDALNATK (SEQ ID NO: 222) EGVITISDGK (SEQ ID NO: 223) GRNVVIEQSFGAPK (SEQ ID NO: 224) GVEELADAVK (SEQ ID NO: 225) IGGASEAEVGEK (SEQ ID NO: 226) IGVQIIQNALK (SEQ ID NO: 227) ISSINAIVK (SEQ ID NO: 228) LLEQENHDLGYDAAK (SEQ ID NO: 229) LQTANFDQK (SEQ ID NO: 230) LSGGVAVLK (SEQ ID NO: 231) In addition, for spot 10α, the mass data obtained from the mass spectrometer was analyzed by collating it with the protein data of NCBI, and it was found that chaperonin CPN60-2, mitochondrial (amino acid sequence: SEQ ID NO: 204, base sequence encoding same: SEQ ID NO: 203) is a protein containing any of SEQ ID NOs: 205 to 218, and chaperonin CPN60-2, mitochondrial is a protein containing any of SEQ ID NOs: 221 to 231. Chaperonin CPN60-2, mitochondrial-like isoform X1 (amino acid sequence: SEQ ID NO: 220, base sequence encoding it: SEQ ID NO: No. 219) was identified.

[0122] Therefore, in the present application, the antigen of spot 10α may be any of the proteins selected from the group consisting of (10αA1-a) to (10αA1-e), (10αB1), (10αA2-a) to (10αA2-e), and (10αB2). (10αA1-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 204. (10αA1-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 204. (10αA1-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 203 have been deleted, substituted, inserted or added. (10αA1-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 203. (10αA1-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 203. (10αB1) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 205 to 218, preferably at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, or all of said amino acid sequences, wherein one or several amino acids may be deleted, substituted, inserted, or added in any of the amino acid sequences shown in SEQ ID NOs: 205 to 218. (10αA2-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 220. (10αA2-b) has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, or 98% or more with the amino acid sequence shown in SEQ ID NO: 220. A protein that contains more than 8% and more than 99% of the amino acid sequence. (10αA2-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 219 have been deleted, substituted, inserted or added. (10αA2-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 219. (10αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 219. (10αB2) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 221 to 231, preferably at least 2, 3, 4, 5, 6, 7, 8, 9, 10, or all of the amino acid sequences, wherein one or several amino acids may be deleted, substituted, inserted, or added in the amino acid sequence represented by any of SEQ ID NOs: 221 to 231.

[0123] The proteins selected from the group consisting of (10αA1-a) to (10αA1-e), (10αB1), (10αA2-a) to (10αA2-e), and (10αB2) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, etc.

[0124] The protein selected from the group consisting of (10αA1-a) to (10αA1-e), (10αB1), (10αA2-a) to (10αA2-e), and (10αB2) may be a protein that appears as a spot with a molecular weight of about 30 to 90 kDa, preferably a molecular weight of about 40 to 80 kDa, and an isoelectric point of 3.5 to 7.5, preferably 4.0 to 7.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the above section ``Identification of antigen.''

[0125] Preferably, the protein that is an antigen of spot 10α has activity equivalent to that of a protein having the amino acid sequence of SEQ ID NO: 204 (haperonin CPN60-2, mitochondrial) or a protein having the amino acid sequence of SEQ ID NO: 220 (chaperonin CPN60-2, mitochondrial-like isoform X1), or causes allergy to soybean.

[0126] (11α) Spot 11α antigen As a result of sequence identification by mass spectrometry for spot 11α, the following amino acid sequence was detected. AIDVLNFTPLNGK (SEQ ID NO: 234) FNNVFVK (SEQ ID NO: 235) GFGFVNFANVDDAAK (sequence number 236) SGAANVFIK (sequence number 237) In addition, when mass data obtained from a mass spectrometer for spot 11α was analyzed by comparing it with NCBI protein data, polyadenylate-binding protein 8 isoform X3 (amino acid sequence: SEQ ID NO: 233, base sequence encoding same: SEQ ID NO: 232) was identified as a protein containing any of SEQ ID NOs: 234 to 237.

[0127] Therefore, in the present application, the antigen of spot 11α may be any protein selected from the group consisting of the following (11αA-a) to (11αA-e) and (11αB). (11αA-a) A protein comprising an amino acid sequence in which one or more amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 233. (11αA-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 233. (11αA-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides in SEQ ID NO: 232 have been deleted, substituted, inserted or added. (11αA-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 232. (11αA-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 232. (11αB) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 234 to 237, preferably at least two, three, or all of the amino acid sequences, wherein the amino acid sequence shown in any of SEQ ID NOs: 234 to 237 may have one or several amino acids deleted, substituted, inserted, or added.

[0128] Proteins selected from the group consisting of (11αA-a) to (11αA-e) and (11αB) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, etc.

[0129] The protein selected from the group consisting of (11αA-a) to (11αA-e) and (11αB) above may be a protein that appears as a spot with a molecular weight of 30 to 90 kDa, preferably a molecular weight of about 40 to 80 kDa, and an isoelectric point of 3.5 to 8.5, preferably 4.0 to 8.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above.

[0130] Preferably, the protein that is an antigen of spot 11α has activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 233 (polyadenylate-binding protein 8 isoform X3), or causes allergy to soybean.

[0131] (12α) Spot 12α antigen As a result of sequence identification by mass spectrometry for spot 12α, the following amino acid sequence was detected. VVGVPVTLNGDLK (SEQ ID NO: 240) In addition, the mass data obtained from the mass spectrometer for spot 12α was analyzed by collating it with the protein data of NCBI, and the protein containing SEQ ID NO: 240 was identified as pyrophosphate-fructose 6-phosphate 1-phosphorotransferase subunit alpha-like (amino acid sequence: SEQ ID NO: 239, base sequence encoding it: SEQ ID NO: 240). Column number 238), was identified.

[0132] Therefore, in the present application, the antigen of spot 12α may be any protein selected from the group consisting of (12αA-a) to (12αA-e) and (12αB) below. (12αA-a) A protein comprising an amino acid sequence in which one or more amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 239. (12αA-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 239. (12αA-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides in SEQ ID NO: 238 have been deleted, substituted, inserted or added. (12αA-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 238. (12αA-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 238. (12αB) A protein comprising the amino acid sequence shown in SEQ ID NO: 240. In this case, the amino acid sequence shown in SEQ ID NO: 240 may have one or several amino acids deleted, substituted, inserted or added.

[0133] Proteins selected from the group consisting of (12αA-a) to (12αA-e) and (12αB) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, etc.

[0134] The protein selected from the group consisting of (12αA-a) to (12αA-e) and (12αB) may be a protein that appears as a spot with a molecular weight of 30 to 90 kDa, preferably a molecular weight of around 40 to 80 kDa, and an isoelectric point of 3.5 to 8.5, preferably 4.0 to 8.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above.

[0135] Preferably, the protein that is the antigen of spot 12α has an activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 239 (pyrophosphate-fructose 6-phosphate 1-phosphotransferase subunit alpha-like), or is a cause of allergy to soybeans. do.

[0136] (13α) Spot 13α antigen As a result of sequence identification by mass spectrometry for spot 13α, the following amino acid sequence was detected. AASILSSHDPPVVLAK (SEQ ID NO: 243) DGNVAPFVK (SEQ ID NO: 244) DLASQYDVRGYPTIK (sequence number 245) EDIIEFIEK (SEQ ID NO: 246) EEQVPLIIIQHNDGK (SEQ ID NO: 247) EFVLTLDHSNFHDTVSK (SEQ ID NO: 248) FFKPNLEADHIPTWLK (SEQ ID NO: 249) FFNSPNAK (SEQ ID NO: 250) FSGEEFDNFSALAEK (SEQ ID NO: 251) FVEESSTPVVTVFNNDPSNHPFVAK (SEQ ID NO: 252) AASILSSHDPPIVLAK (SEQ ID NO: 255) DGHVAPFVK (SEQ ID NO: 256) DLASQYDVK (sequence number 257) EDIIEFIEK (SEQ ID NO: 258) EEQVPLIIIQHNDGK (SEQ ID NO: 259) EFVLTLDHSNFHDTVSK (SEQ ID NO: 260) FFKPNLEADHIPTWLK (SEQ ID NO: 261) FFNSPNAK (SEQ ID NO: 262) FSGEEFDNFSALAEK (SEQ ID NO: 263) FVEESSTPVVTVFNNEPSNHPFVVK (SEQ ID NO: 264) GFPTINILRNGGK (SEQ ID NO: 265) GPREADGIVDYLK (SEQ ID NO: 266) LAPEYEK (SEQ ID NO: 267) LDATANDIPSETFDVQGYPTVYFRSASGK (SEQ ID NO: 268) LRSDYDFGHTLNAK (SEQ ID NO: 269) LSQYDGGRTK (SEQ ID NO: 270) QLAPILDEVAISYQNEADVVIAK (SEQ ID NO: 271) QQGVSFLVGDVESSQGAFQYFGLK (SEQ ID NO: 272) QSGPASTEIK (SEQ ID NO: 273) SADEATAFIGENK (SEQ ID NO: 274) SEPIPETNDEPVK (SEQ ID NO: 275) VAIVGVFPK (SEQ ID NO: 276) VVVGASLEDIVFK (sequence number 277) YREAAEQYK (SEQ ID NO: 278) In addition, for spot 13α, the mass data obtained from the mass spectrometer was analyzed by collating it with the protein data of NCBI, and the proteins containing any of SEQ ID NOs: 243 to 252 were identified as protein disulfide isomerase-like protein precursor (amino acid sequence: SEQ ID NO: 242, base sequence encoding same: SEQ ID NO: 241), and the proteins containing any of SEQ ID NOs: 255 to 278 were identified as protein disulfide isomerase (amino acid sequence: SEQ ID NO: 254, base sequence encoding same: SEQ ID NO: 255). 3) was identified.

[0137] Therefore, in the present application, the antigen of spot 13α may be any protein selected from the group consisting of (13αA1-a) to (13αA1-e), (13αB1), (13αA2-a) to (13αA2-e), and (13αB2). (13αA1-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 242. (13αA1-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 242. (13αA1-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 241 have been deleted, substituted, inserted or added. (13αA1-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 241. (13αA1-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 241. (13αB1) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 243 to 252, preferably at least 2, 3, 4, 5, 6, 7, 8, 9, or all of the amino acid sequences, wherein one or several amino acids may be deleted, substituted, inserted, or added in the amino acid sequence represented by any of SEQ ID NOs: 243 to 252. (13αA2-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 254. (13αA2-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 254. (13αA2-c) One or several nucleotides are deleted, substituted or inserted in SEQ ID NO: 253 A protein comprising an amino acid sequence encoded by an inserted or added base sequence. (13αA2-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 253. (13αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 253. (13αB2) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 255 to 278, preferably at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, or all of the amino acid sequences. Here, the amino acid sequence shown in any of SEQ ID NOs: 255 to 278 may have one or several amino acids deleted, substituted, inserted, or added.

[0138] The proteins selected from the group consisting of (13αA1-a) to (13αA1-e), (13αB1), (13αA2-a) to (13αA2-e), and (13αB2) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, etc.

[0139] The protein selected from the group consisting of (13αA1-a) to (13αA1-e), (13αB1), (13αA2-a) to (13αA2-e), and (13αB2) may be a protein that appears as a spot with a molecular weight of about 30 to 90 kDa, preferably a molecular weight of about 40 to 80 kDa, and an isoelectric point of 3.5 to 7.5, preferably 4.0 to 7.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above.

[0140] Preferably, the protein that is the antigen of spot 13α is a protein having the amino acid sequence of SEQ ID NO: 242 (protein disulfide isomerase-like protein precursor) or a protein having the amino acid sequence of SEQ ID NO: 254 (protein disulfide isomerase). It has activity equivalent to that of soybeans or causes allergies to soybeans.

[0141] (14α) Spot 14α antigen As a result of sequence identification by mass spectrometry for spot 14α, the following amino acid sequence was detected. DHSDVSNQLYANYAIGK (SEQ ID NO: 281) FQEIVNIRLGDGTTRRGQVLEVDGEK (SEQ ID NO: 282) FTTVQFTGEVLK (sequence number 283) TLDQFYSRDASN (SEQ ID NO: 284) FQEIVNIRLGDGTTRRGQVLEVDGEK (SEQ ID NO: 287) FTTVQFTGEVLK (sequence number 288) TLDQFYSRDAGN (SEQ ID NO: 289) In addition, when the mass data obtained from a mass spectrometer for spot 14α was analyzed by comparing it with NCBI protein data, V-type proton ATPase subunit B 2 (amino acid sequence: SEQ ID NO: 280, nucleotide sequence encoding same: SEQ ID NO: 279) was identified as a protein containing any of SEQ ID NOs: 281 to 284, and V-type proton ATPase subunit B2 (amino acid sequence: SEQ ID NO: 286, nucleotide sequence encoding same: SEQ ID NO: 285) was identified as a protein containing any of SEQ ID NOs: 287 to 289.

[0142] Therefore, in the present application, the antigens of spot 14α are as follows: (14αA1-a) to (14 The protein may be any one of proteins selected from the group consisting of (14αA1-e), (14αB1), (14αA2-a) to (14αA2-e), and (14αB2). (14αA1-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 280. (14αA1-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 280. (14αA1-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 279 have been deleted, substituted, inserted or added. (14αA1-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 279. (14αA1-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 279. (14αB1) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 281 to 284, preferably at least two, three, or all of the amino acid sequences, wherein the amino acid sequence shown in any of SEQ ID NOs: 281 to 284 may have one or several amino acids deleted, substituted, inserted, or added. (14αA2-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 286. (14αA2-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 286. (14αA2-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 285 have been deleted, substituted, inserted or added. (14αA2-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 285. (14αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 285. (14αB2) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 287 to 289, preferably at least two or all of the amino acid sequences, wherein the amino acid sequence shown in any of SEQ ID NOs: 287 to 289 may have one or several amino acids deleted, substituted, inserted or added.

[0143] The proteins selected from the group consisting of (14αA1-a) to (14αA1-e), (14αB1), (14αA2-a) to (14αA2-e), and (14αB2) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, etc.

[0144] The protein selected from the group consisting of (14αA1-a) to (14αA1-e), (14αB1), (14αA2-a) to (14αA2-e), and (14αB2) may be a protein that appears as a spot with a molecular weight of about 30 to 90 kDa, preferably a molecular weight of about 40 to 80 kDa, and an isoelectric point of 2.5 to 6.5, preferably 3.0 to 6.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the above section ``Identification of antigen.''

[0145] Preferably, the protein that is an antigen of spot 14α has activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 280 or 286 (V-type proton ATPase subunit B 2), or causes allergy to soybean.

[0146] (15α, 16α) Spot 15α, 16α antigens As a result of sequence identification by mass spectrometry for spots 15α and 16α, the following amino acid sequences were detected. AIGINVPRSRFLPVK (SEQ ID NO: 292) ARENPENPSIELGPEFK (SEQ ID NO: 293) ATSDLLLVQSDLYTLEDGFVIRNK (SEQ ID NO: 294) EINGPEDL (SEQ ID NO: 295) EYVFVANSDNLGAIVDLK (SEQ ID NO: 296) ILNHLIQNK (SEQ ID NO: 297) IQTPTDEVVVPYDTLAPTPEGSSEVK (SEQ ID NO: 298) LDALLSQGK (sequence number 299) RLVEADALK (SEQ ID NO: 300) SAVAGLNEISENEK (SEQ ID NO: 301) SIPSIVELDSLK (SEQ ID NO: 302) VLQLETAAGAAIRFFDK (SEQ ID NO: 303) VSNFLGRFK (SEQ ID NO: 304) AIGINVPRSRFLPVK (SEQ ID NO: 307) ATSDLLLVQSDLYTLEDGFVIRNK (sequence number 308) EYVFVANSDNLGAIVDLK (SEQ ID NO: 309) ILNHLIQNK (SEQ ID NO: 310) IQTPTDEVVVPYDTLAPTPDGSSDVK (SEQ ID NO: 311) LDALLSQGK (SEQ ID NO: 312) LEVPDGAVISDK (SEQ ID NO: 313) RLVEADALK (SEQ ID NO: 314) SAVAGLNEISESEK (SEQ ID NO: 315) SIPSIVELDSLK (SEQ ID NO: 316) VLQLETAAGAAIRFFDK (SEQ ID NO: 317) VSNFLGRFK (SEQ ID NO: 318) In addition, for spots 15α and 16α, the mass data obtained from a mass spectrometer was analyzed by collating it with the protein data of NCBI, and it was found that UTP-glucose-1-phosphate uridylyltransferase (amino acid sequence: SEQ ID NO: 2) was a protein containing any one of SEQ ID NOs: 292 to 304. 91, a base sequence encoding it: SEQ ID NO: 290), and a protein comprising any one of SEQ ID NOs: 307 to 318, hypothetical protein GLYMA_02G241100 (amino acid sequence: SEQ ID NO: 306, a base sequence encoding it: SEQ ID NO: 307 to 318) Array: SEQ ID NO: 305) was identified.

[0147] Therefore, in the present application, the antigens of spots 15α and 16α may be any of the proteins selected from the group consisting of (15,16αA1-a) to (15,16αA1-e), (15,16αB1), (15,16αA2-a) to (15,16αA2-e), and (15,16αB2). (15,16αA1-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 291. (15,16αA1-b) The amino acid sequence shown in SEQ ID NO: 291 has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, or 97% or more. A protein that contains more than 98% or more than 99% of the amino acid sequence. (15,16αA1-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 290 have been deleted, substituted, inserted or added. (15,16αA1-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 290. (15,16αA1-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO:290. (15,16αB1) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 292 to 304, preferably at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, or all of the amino acid sequences, wherein one or several amino acids may be deleted, substituted, inserted, or added in the amino acid sequence shown in any of SEQ ID NOs: 292 to 304. (15,16αA2-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 306. (15,16αA2-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 306. (15.16αA2-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 305 have been deleted, substituted, inserted or added. (15,16αA2-d) A protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the nucleotide sequence shown in SEQ ID NO: 305. (15,16αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 305. (15,16αB2) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 307 to 318, preferably a protein comprising at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, or all of said amino acid sequences, wherein one or several amino acids may be deleted, substituted, inserted, or added in any of the amino acid sequences shown in SEQ ID NOs: 307 to 318.

[0148] The proteins selected from the group consisting of (15,16αA1-a) to (15,16αA1-e), (15,16αB1), (15,16αA2-a) to (15,16αA2-e), and (15,16αB2) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, etc.

[0149] The protein selected from the group consisting of (15,16αA1-a) to (15,16αA1-e), (15,16αB1), (15,16αA2-a) to (15,16αA2-e), and (15,16αB2) may be a protein that appears as a spot with a molecular weight of about 20 to 90 kDa, preferably a molecular weight of about 30 to 80 kDa, and an isoelectric point of 3.5 to 7.5, preferably 4.0 to 7.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the above section ``Identification of antigen.''

[0150] Preferably, the protein that is the antigen of spots 15α and 16α is a protein having the amino acid sequence of SEQ ID NO: 291 (UTP-glucose-1-phosphate uridylyltransferase) or or has activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 306 (hypothetical protein GLYMA_02G241100), or causes allergy to soybeans.

[0151] (17α) Spot 17α antigen As a result of sequence identification by mass spectrometry for spot 17α, the following amino acid sequence was detected. AVDGSFVFNK (SEQ ID NO: 321) FFIYVQNYNESDPK (sequence number 322) VEFDEEGK (SEQ ID NO: 323) VIDLSVDLSAASAAEE (SEQ ID NO: 324) VVGVTSEGETAK (SEQ ID NO: 325) AVDGSYVFNK (sequence number 328) FFIYVQNYNESDPK (sequence number 329) VEFDEEGK (SEQ ID NO: 330) VIDLSVDLSAASAAEE (SEQ ID NO: 331) VVGVTSEGETAK (SEQ ID NO: 332) In addition, the mass data obtained from the mass spectrometer for spot 17α was analyzed by collating it with the protein data of NCBI. As a result, the protein containing any one of SEQ ID NOs: 321 to 325 was identified as guanosine nucleotide diphosphate dissociation inhibitor 2 (amino acid sequence: As a protein containing any one of SEQ ID NO: 320, the base sequence encoding it: SEQ ID NO: 319, and SEQ ID NO: 328 to 332, rab GDP dissociation inhibitor alpha-like (amino acid sequence: SEQ ID NO: No. 327, and the base sequence encoding it: SEQ ID NO: 326) was identified.

[0152] Therefore, in the present application, the antigen of spot 17α may be any of the proteins selected from the group consisting of (17αA1-a) to (17αA1-e), (17αB1), (17αA2-a) to (17αA2-e), and (17αB2). (17αA1-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 320. (17αA1-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 320. (17αA1-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 319 have been deleted, substituted, inserted or added. (17αA1-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 319. (17αA1-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 319. (17αB1) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 321 to 325, preferably at least two, three, four, or all of the amino acid sequences, wherein the amino acid sequence shown in any of SEQ ID NOs: 321 to 325 may have one or several amino acids deleted, substituted, inserted, or added. (17αA2-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 327. (17αA2-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 327. (17αA2-c) One or several nucleotides are deleted, substituted or inserted in SEQ ID NO: 326 A protein comprising an amino acid sequence encoded by an inserted or added base sequence. (17αA2-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 326. (17αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 326. (17αB2) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 328 to 332, preferably at least two, three, four, or all of the amino acid sequences, wherein the amino acid sequence shown in any of SEQ ID NOs: 328 to 332 may have one or several amino acids deleted, substituted, inserted, or added.

[0153] The proteins selected from the group consisting of (17αA1-a) to (17αA1-e), (17αB1), (17αA2-a) to (17αA2-e), and (17αB2) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, etc.

[0154] The protein selected from the group consisting of (17αA1-a) to (17αA1-e), (17αB1), (17αA2-a) to (17αA2-e), and (17αB2) may be a protein that appears as a spot with a molecular weight of 20 to 90 kDa, preferably a molecular weight of about 30 to 80 kDa, and an isoelectric point of 3.5 to 7.5, preferably 4.0 to 7.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above.

[0155] Preferably, the protein that is the antigen of spot 17α is a protein having the amino acid sequence of SEQ ID NO: 320 (guanosine nucleotide diphosphate dissociation inhibitor 2 ) or a protein having the amino acid sequence of SEQ ID NO: 327 (rab GDP dissociation inhibitor alpha-like), or a protein that is a cause of soy allergy. become.

[0156] (18α) Spot 18α antigen As a result of sequence identification by mass spectrometry for spot 18α, the following amino acid sequence was detected. DFETRVETEGGRIRVLK (SEQ ID NO: 335) DNIVSSLDNVAK (SEQ ID NO: 336) GRAVLGLVSESETEK (SEQ ID NO: 337) LFDQQNEGSIFAISREQVRALAPTK (SEQ ID NO: 338) RPTISNGYGRLTEVGPDDDEK (SEQ ID NO: 339) In addition, for spot 18α, the mass data obtained from a mass spectrometer was analyzed by collating it with the protein data of NCBI, and it was found to be a protein containing any one of SEQ ID NOs: 335 to 339, namely hypothetical protein GLYMA_10G028300 (amino acid sequence: SEQ ID NO: 334, base sequence encoding it: SEQ ID NO: 335). No. 333), was identified.

[0157] Therefore, in the present application, the antigen of spot 18α may be any of the proteins selected from the group consisting of (18αA-a) to (18αA-e) and (18αB) below. (18αA-a) A protein comprising an amino acid sequence in which one or more amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 334. (18αA-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 334. (18αA-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides in SEQ ID NO: 333 have been deleted, substituted, inserted or added. (18αA-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 333. (18αA-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 333. (18αB) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 335 to 339, preferably at least two, three, four, or all of the amino acid sequences, wherein the amino acid sequence shown in any of SEQ ID NOs: 335 to 339 may have one or several amino acids deleted, substituted, inserted, or added.

[0158] Proteins selected from the group consisting of (18αA-a) to (18αA-e) and (18αB) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, etc.

[0159] The protein selected from the group consisting of (18αA-a) to (18αA-e) and (18αB) may be a protein that appears as a spot with a molecular weight of 30 to 90 kDa, preferably a molecular weight of around 40 to 80 kDa, and an isoelectric point of 3.5 to 8.5, preferably 4.0 to 8.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above.

[0160] Preferably, the protein that is an antigen of spot 18α has activity equivalent to the protein having the amino acid sequence of SEQ ID NO: 334 (hypothetical protein GLYMA_10G028300), or causes allergy to soybean.

[0161] (19α) Spot 19α antigen As a result of sequence identification by mass spectrometry for spot 19α, the following amino acid sequence was detected. AQFNIFSK (sequence number 342) DFSTRVETEGGSIRVLK (SEQ ID NO: 343) DNIVSSLDNVAK (SEQ ID NO: 344) GRAVLGLVRESETEK (SEQ ID NO: 345) RPTFSNGYGRLTEVGPDDEK (SEQ ID NO: 346) In addition, when the mass data obtained from a mass spectrometer for spot 19α was analyzed by comparing it with NCBI protein data, sucrose binding protein homolog S-64 (amino acid sequence: SEQ ID NO: 341, base sequence encoding it: SEQ ID NO: 340) was identified as a protein containing any of SEQ ID NOs: 342 to 346.

[0162] Therefore, in the present application, the antigen of spot 19α may be any protein selected from the group consisting of (19αA-a) to (19αA-e) and (19αB) below. (19αA-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 341. (19αA-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 341. (19αA-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides in SEQ ID NO: 340 have been deleted, substituted, inserted or added. (19αA-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 340. (19αA-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 340. (19αB) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 342 to 346, preferably at least two, three, four, or all of the amino acid sequences, wherein the amino acid sequence shown in any of SEQ ID NOs: 342 to 346 may have one or several amino acids deleted, substituted, inserted, or added.

[0163] Proteins selected from the group consisting of (19αA-a) to (19αA-e) and (19αB) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, etc.

[0164] The protein selected from the group consisting of (19αA-a) to (19αA-e) and (19αB) may be a protein that appears as a spot with a molecular weight of 30 to 90 kDa, preferably a molecular weight of around 40 to 80 kDa, and an isoelectric point of 3.5 to 8.5, preferably 4.0 to 8.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above.

[0165] Preferably, the protein that is an antigen of spot 19α has activity equivalent to the protein having the amino acid sequence of SEQ ID NO: 341 (sucrose binding protein homolog S-64) or causes allergy to soybean.

[0166] (25α) Spot 25α antigen As a result of sequence identification by mass spectrometry for spot 25α, the following amino acid sequence was detected. DLFPNENELVVK (SEQ ID NO: 349) EIFSLRDTTQEDPREVTAAK (SEQ ID NO: 350) LHGGTPANFLDVGGNASENQVVEAFK (SEQ ID NO: 351) LNFDDNAAYRQK (SEQ ID NO: 352) SQILAGGRGLGTFK (SEQ ID NO: 353) TDQVEDIAGK (SEQ ID NO: 354) VPVVVRLEGTNVDQGK (SEQ ID NO: 355) VVDGLALK (SEQ ID NO: 356) DLFPNENELVVK (SEQ ID NO: 359) EIFALRDTTQEDPREVTAAK (SEQ ID NO: 360) LHGGTPANFLDVGGNASEGQVVEAFK (SEQ ID NO: 361) LNFDDNAAYRQK (SEQ ID NO: 362) SQILAGGRGLGTFK (SEQ ID NO: 363) TDQVEDIAGK (SEQ ID NO: 364) VVDGLALK (SEQ ID NO: 365) In addition, the mass data obtained from the mass spectrometer for spot 25α was uploaded to the NCBI database. Analysis of the protein data revealed that the protein containing any of SEQ ID NOs: 349 to 356 was succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial (amino acid sequence: SEQ ID NO: 348, base sequence encoding same: SEQ ID NO: 347). As a protein containing any one of SEQ ID NOs: 359 to 365, succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial (amino acid sequence: SEQ ID NO: 358, base sequence encoding same: SEQ ID NO: 357) was identified.

[0167] Therefore, in the present application, the antigen of spot 25α may be any of the proteins selected from the group consisting of (25αA1-a) to (25αA1-e), (25αB1), (25αA2-a) to (25αA2-e), and (25αB2). (25αA1-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 348. (25αA1-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 348. (25αA1-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 347 have been deleted, substituted, inserted or added. (25αA1-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 347. (25αA1-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 347. (25αB1) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 349 to 356, preferably at least 2, 3, 4, 5, 6, 7, or all of the amino acid sequences, wherein one or several amino acids may be deleted, substituted, inserted, or added in the amino acid sequence represented by any of SEQ ID NOs: 349 to 356. (25αA2-a) A protein comprising an amino acid sequence in which one or more amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 358. (25αA2-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 358. (25αA2-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 357 have been deleted, substituted, inserted or added. (25αA2-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 357. (25αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 357. (25αB2) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 359 to 365, preferably at least 2, 3, 4, 5, 6, or all of the amino acid sequences, wherein the amino acid sequence shown in any of SEQ ID NOs: 359 to 365 may have one or several amino acids deleted, substituted, inserted, or added.

[0168] The protein selected from the group consisting of (25αA1-a) to (25αA1-e), (25αB1), (25αA2-a) to (25αA2-e), and (25αB2) Also included are proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, and the like.

[0169] The protein selected from the group consisting of (25αA1-a) to (25αA1-e), (25αB1), (25αA2-a) to (25αA2-e), and (25αB2) may be a protein that appears as a spot with a molecular weight of about 20 to 90 kDa, preferably a molecular weight of about 30 to 80 kDa, and an isoelectric point of 3.5 to 7.5, preferably 4.0 to 7.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the above section ``Identification of antigen.''

[0170] Preferably, the protein that is an antigen of spot 25α has an activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 348 or 358 (succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial), or is a cause of allergy to soybeans. .

[0171] (26α) Spot 26α antigen As a result of sequence identification by mass spectrometry for spot 26α, the following amino acid sequence was detected. AQGYSVGSSLVEEDK (sequence number 368) ELDYLVGAVSNPK (SEQ ID NO: 369) FAADANSK (SEQ ID NO: 370) FATGTEAIAK (SEQ ID NO: 371) GVSLLLPTDVVIADK (SEQ ID NO: 372) GVTTIIGGGDSVAAVEK (SEQ ID NO: 373) IGVIESLLEK (SEQ ID NO: 374) LASLADLYVNDAFGTAHRAHASTEGVAK (SEQ ID NO: 375) LSLATTLLEK (sequence number 376) LVTQLPEGGVLLLENVRFYK (SEQ ID NO: 377) NDPEFAK (SEQ ID NO: 378) QLPGVLALDDA (SEQ ID NO: 379) RPFAAIVGGSK (SEQ ID NO:380) VILSSHLGRPK (SEQ ID NO: 381) YLTGYGAK (SEQ ID NO: 382) ELDYLVGAVSNPK (SEQ ID NO: 385) ELPGVLALDEAVPVAV (SEQ ID NO: 386) FAVGTEAIAK (SEQ ID NO: 387) GVSLLLPSDVVIADK (SEQ ID NO: 388) GVTTIIGGGDSVAAVEK (SEQ ID NO: 389) IGVIESLLEK (SEQ ID NO: 390) RPFAAIVGGSK (SEQ ID NO:391) VILSSHLGRPK (SEQ ID NO: 392) AQGYSVGSSLVEEDK (sequence number 395) ELDYLVGAVSNPK (SEQ ID NO: 396) FAADANSK (SEQ ID NO: 397) FATGTEAIAK (SEQ ID NO: 398) GVSLLLPTDVVIADK (SEQ ID NO: 399) GVTTIIGGGDSVAAVEK (SEQ ID NO: 400) IGVIESLLEK (sequence number 401) LASLADLYVNDAFGTAHRAHASTEGVAK (SEQ ID NO: 402) LSLATTLLEK (sequence number 403) LVAQLPEGGVLLLENVRFYK (SEQ ID NO: 404) NDPEFAK (SEQ ID NO: 405) QLPGVLALDDA (SEQ ID NO: 406) RPFAAIVGGSK (SEQ ID NO: 407) TFGEALDK (sequence number 408) VILSSHLGRPK (sequence number 409) In addition, for spot 26α, mass data obtained from a mass spectrometer was analyzed by collating it with NCBI protein data, and phosphoglycerate kinase, cytosolic (amino acid sequence: SEQ ID NO: 367, base sequence encoding same: SEQ ID NO: 366) was identified as a protein containing any of SEQ ID NOs: 368 to 382, ​​phosphoglycerate kinase, cytosolic (amino acid sequence: SEQ ID NO: 384, base sequence encoding same: SEQ ID NO: 383) was identified as a protein containing any of SEQ ID NOs: 385 to 392, and phosphoglycerate kinase, cytosolic (amino acid sequence: SEQ ID NO: 394, base sequence encoding same: SEQ ID NO: 393) was identified as a protein containing any of SEQ ID NOs: 395 to 409.

[0172] Therefore, in the present application, the antigen of spot 26α may be any of the proteins selected from the group consisting of (26αA1-a) to (26αA1-e), (26αB1), (26αA2-a) to (26αA2-e), (26αB2), (26αA3-a) to (26αA3-e), and (26αB3). (26αA1-a) A protein comprising an amino acid sequence in which one or more amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 367. (26αA1-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 367. (26αA1-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 366 have been deleted, substituted, inserted or added. (26αA1-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 366. (26αA1-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 366. (26αB1) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 368 to 382, ​​preferably at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or all of said amino acid sequences, wherein one or several amino acids may be deleted, substituted, inserted, or added in any of the amino acid sequences shown in SEQ ID NOs: 368 to 382. (26αA2-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 384. (26αA2-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 384. (26αA2-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides in SEQ ID NO: 383 have been deleted, substituted, inserted or added. (26αA2-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 383. (26αA2-e) Amino acid encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 383 A protein containing the sequence. (26αB2) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 385 to 392, preferably at least 2, 3, 4, 5, 6, 7, or all of the amino acid sequences, wherein one or several amino acids may be deleted, substituted, inserted, or added in the amino acid sequence represented by any of SEQ ID NOs: 385 to 392. (26αA3-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 394. (26αA3-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 394. (26αA3-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 393 have been deleted, substituted, inserted or added. (26αA3-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 393. (26αA3-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 393. (26αB3) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 395 to 409, preferably at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or all of the amino acid sequences, wherein one or several amino acids may be deleted, substituted, inserted, or added in the amino acid sequence shown in any of SEQ ID NOs: 395 to 409.

[0173] The proteins selected from the group consisting of (26αA1-a) to (26αA1-e), (26αB1), (26αA2-a) to (26αA2-e), (26αB2), (26αA3-a) to (26αA3-e), and (26αB3) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, etc.

[0174] The protein selected from the group consisting of (26αA1-a) to (26αA1-e), (26αB1), (26αA2-a) to (26αA2-e), (26αB2), (26αA3-a) to (26αA3-e), and (26αB3) may be a protein that appears as a spot with a molecular weight of 20 to 90 kDa, preferably a molecular weight of about 30 to 80 kDa, and an isoelectric point of 3.5 to 7.5, preferably 4.0 to 7.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above.

[0175] Preferably, the protein that is an antigen of spot 25α has activity equivalent to the protein having the amino acid sequence of SEQ ID NO: 367, 384 or 394 (phosphoglycerate kinase, cytosolic), or causes allergy to soybean.

[0176] (27α) Spot 27α antigen As a result of sequence identification by mass spectrometry for spot 27α, the following amino acid sequence was detected. FGVNEFVNPK (SEQ ID NO: 412) FITHTVPFSEINK (SEQ ID NO: 413) GTFYGNYKPRTDLPSVVEK (SEQ ID NO: 414) THPVNFLNERTLK (SEQ ID NO: 415) VNPAAPLDK (SEQ ID NO: 416) GTFYGHYRPRTDIPGVVEK (SEQ ID NO: 419) INPNAPLDK (SEQ ID NO: 420) FGVNEFVNPK (SEQ ID NO: 423) FITHTVPFSEINK (SEQ ID NO: 424) GTFYGNYKPRTDLPSVVEK (SEQ ID NO: 425) INPAAPLDK (SEQ ID NO: 426) TAPINFLNERTLK (SEQ ID NO: 427) In addition, the mass data obtained from the mass spectrometer for spot 27α was analyzed by collating it with the protein data of NCBI, and it was found that alcohol dehydrogenase 1 (amino acid sequence 412 to 416) was a protein containing any one of SEQ ID NOs: 412 to 416. Alcohol dehydrogenase 1 was identified as a protein containing any of SEQ ID NOs: 411 (sequence: SEQ ID NO: 411, and the base sequence encoding it: SEQ ID NO: 410), SEQ ID NOs: 419 and 420 (amino acid sequence: SEQ ID NO: 418, and the base sequence encoding it: SEQ ID NO: 417), and alcohol dehydrogenase 1-like was identified as a protein containing any of SEQ ID NOs: 423 to 427 (amino acid sequence: SEQ ID NO: 422, and the base sequence encoding it: SEQ ID NO: 421).

[0177] Therefore, in the present application, the antigen of spot 27α may be any of the proteins selected from the group consisting of (27αA1-a) to (27αA1-e), (27αB1), (27αA2-a) to (27αA2-e), (27αB2), (27αA3-a) to (27αA3-e), and (27αB3). (27αA1-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 411. (27αA1-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 411. (27αA1-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 410 have been deleted, substituted, inserted or added. (27αA1-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 410. (27αA1-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 410. (27αB1) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 412 to 416, preferably at least two, three, four, or all of the amino acid sequences, wherein the amino acid sequence shown in any of SEQ ID NOs: 412 to 416 may have one or several amino acids deleted, substituted, inserted, or added. (27αA2-a) A protein comprising an amino acid sequence in which one or more amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 418. (27αA2-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 418. (27αA2-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides in SEQ ID NO: 417 have been deleted, substituted, inserted or added. (27αA2-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 417. (27αA2-e) A base sequence complementary to the base sequence shown in SEQ ID NO: 417 A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with the nucleic acid. (27αB2) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 419 and 420, preferably comprising both of said amino acid sequences. In this regard, the amino acid sequence shown in either SEQ ID NO: 419 or 420 may have one or several amino acids deleted, substituted, inserted or added. (27αA3-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 422. (27αA3-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 422. (27αA3-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 421 have been deleted, substituted, inserted or added. (27αA3-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 421. (27αA3-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 421. (27αB3) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 423 to 427, preferably at least two, three, four, or all of the amino acid sequences, wherein the amino acid sequence shown in any of SEQ ID NOs: 423 to 427 may have one or several amino acids deleted, substituted, inserted, or added.

[0178] The proteins selected from the group consisting of (27αA1-a) to (27αA1-e), (27αB1), (27αA2-a) to (27αA2-e), (27αB2), (27αA3-a) to (27αA3-e), and (27αB3) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, etc.

[0179] The protein selected from the group consisting of (27αA1-a) to (27αA1-e), (27αB1), (27αA2-a) to (27αA2-e), (27αB2), (27αA3-a) to (27αA3-e), and (27αB3) may be a protein that appears as a spot with a molecular weight of 20 to 90 kDa, preferably a molecular weight of about 30 to 80 kDa, and an isoelectric point of 3.5 to 7.5, preferably 4.0 to 7.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the above section ``Identification of antigen.''

[0180] Preferably, the protein that is the antigen of spot 27α is a protein having the amino acid sequence of SEQ ID NO: 411 or 418 (alcohol dehydrogenase 1) or SEQ ID NO: 42 It has activity equivalent to that of a protein having the amino acid sequence of 2 (alcohol dehydrogenase 1-like), or causes allergies to soybeans.

[0181] (28α) Spot 28α antigen As a result of sequence identification by mass spectrometry for spot 28α, the following amino acid sequence was detected. AGEYTDYAAQK (SEQ ID NO: 430) DIGDINRTNERDEGYDLHDEANFK (SEQ ID NO: 431) DLEDANRLRDNTFNTWK (SEQ ID NO: 432) EAVVGKPHHEEVYAK (SEQ ID NO: 433) In addition, for spot 28α, the mass data obtained from a mass spectrometer was analyzed by collating it with the protein data of NCBI, and it was found that the protein containing any of SEQ ID NOs: 430 to 433 was 35 kDa seed maturation protein isoform X1 (amino acid sequence: SEQ ID NO: 429, The base sequence corresponding to this gene (SEQ ID NO: 428) was identified.

[0182] Therefore, in the present application, the antigen of spot 28α may be any protein selected from the group consisting of (28αA-a) to (28αA-e) and (28αB) below. (28αA-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 429. (28αA-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 429. (28αA-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides in SEQ ID NO: 428 have been deleted, substituted, inserted or added. (28αA-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 428. (28αA-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 428. (28αB) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 430 to 433, preferably at least two, three, or all of the amino acid sequences, wherein the amino acid sequence shown in any of SEQ ID NOs: 430 to 433 may have one or several amino acids deleted, substituted, inserted, or added.

[0183] Proteins selected from the group consisting of (28αA-a) to (28αA-e) and (28αB) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, etc.

[0184] The protein selected from the group consisting of (28αA-a) to (28αA-e) and (28αB) may be a protein that appears as a spot with a molecular weight of 10 to 70 kDa, preferably a molecular weight of around 20 to 60 kDa, and an isoelectric point of 3.5 to 7.5, preferably 4.0 to 7.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above.

[0185] Preferably, the protein that is an antigen of spot 28α has an activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 422 (35 kDa seed maturation protein isoform X1). It may cause allergies to soy.

[0186] (29α, 30α, 31α, 32α, 33α) Spot 29α-33α antigens As a result of sequence identification by mass spectrometry for spots 29α to 33α, the following amino acid sequences were detected. EASYDEIK (sequence number 436) GILGYTEDDVVSTDFIGDSRSSIFDAK (SEQ ID NO: 437) VIISAPSK (SEQ ID NO: 438) VLPALNGK (SEQ ID NO: 439) EASYDEIK (SEQ ID NO: 442) GILGYTEDDVVSTDFVGDSRSSIFDAK (SEQ ID NO: 443) VIISAPSK (SEQ ID NO: 444) VLPALNGK (SEQ ID NO: 445) AGISLNDNFVK (SEQ ID NO: 448) GILGYTEDDVVSTDFVGDNRSSIFDAK (SEQ ID NO: 449) VLPALNGK (SEQ ID NO: 450) VVISAPSK (SEQ ID NO: 451) In addition, for spots 27α to 33α, the mass data obtained from a mass spectrometer was analyzed by collating it with the protein data of NCBI, and it was found that glyceraldehyde-3-phosphate dehydrogenase isoform X1 (amino acid sequence) was identified as a protein containing any one of SEQ ID NOs: 436 to 439. As a protein containing any one of SEQ ID NOs: 442 to 445, glyceraldehyde-3-phosphate dehydrogenase, cytosolic (glyceraldehyde-3-phosphate dehydrogenase, cytosolic ) (amino acid sequence: SEQ ID NO: 441, base sequence encoding it: SEQ ID NO: 440), and uncharacterized protein LOC100782924 (amino acid sequence: SEQ ID NO: 447, base sequence encoding it: SEQ ID NO: 446) were identified as a protein containing any one of SEQ ID NOs: 448 to 451.

[0187] Therefore, in the present application, the antigens of spots 29α to 33α may be any of the proteins selected from the group consisting of (29-33αA1-a) to (29-33αA1-e), (29-33αB1), (29-33αA2-a) to (29-33αA2-e), (29-33αB2), (29-33αA3-a) to (29-33αA3-e), and (29-33αB3). (29-33αA1-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 435. (29-33αA1-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 435. (29-33αA1-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 434 have been deleted, substituted, inserted or added. (29-33αA1-d) A protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the nucleotide sequence shown in SEQ ID NO: 434. (29-33αA1-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 434. (29-33αB1) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 436 to 439, preferably at least two, three, or all of the amino acid sequences, wherein the amino acid sequence shown in any of SEQ ID NOs: 436 to 439 may have one or several amino acids deleted, substituted, inserted, or added. (29-33αA2-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 441. (29-33αA2-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 441. (29-33αA2-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 440 have been deleted, substituted, inserted or added. (29-33αA2-d) 70% or more identity with the base sequence shown in SEQ ID NO: 440, preferably A protein comprising an amino acid sequence encoded by a nucleotide sequence of preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more. (29-33αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO:440. (29-33αB2) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 442 to 445, preferably at least two, three, or all of the amino acid sequences, wherein the amino acid sequence shown in any of SEQ ID NOs: 442 to 445 may have one or several amino acids deleted, substituted, inserted, or added. (29-33αA3-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 447. (29-33αA3-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 447. (29-33αA3-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 446 have been deleted, substituted, inserted or added. (29-33αA3-d) A protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the nucleotide sequence shown in SEQ ID NO: 446. (29-33αA3-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 446. (29-33αB3) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 448 to 451, preferably at least two, three, or all of the amino acid sequences, wherein the amino acid sequence shown in any of SEQ ID NOs: 448 to 451 may have one or several amino acids deleted, substituted, inserted, or added.

[0188] Proteins selected from the group consisting of (29-33αA1-a) to (29-33αA1-e), (29-33αB1), (29-33αA2-a) to (29-33αA2-e), (29-33αB2), (29-33αA3-a) to (29-33αA3-e), and (29-33αB3) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, etc.

[0189] The protein selected from the group consisting of (29-33αA1-a) to (29-33αA1-e), (29-33αB1), (29-33αA2-a) to (29-33αA2-e), (29-33αB2), (29-33αA3-a) to (29-33αA3-e), and (29-33αB3) may be a protein that appears as a spot with a molecular weight of 10 to 70 kDa, preferably a molecular weight of about 20 to 60 kDa, and an isoelectric point of 3.5 to 8.5, preferably 4.0 to 8.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the above section ``Identification of antigen.''

[0190] Preferably, the protein that is an antigen of spots 29α to 33α has activity equivalent to that of a protein having the amino acid sequence of SEQ ID NO: 435 (glyceraldehyde-3-phosphate dehydrogenase isoform X1), a protein having the amino acid sequence of SEQ ID NO: 441 (glyceraldehyde-3-phosphate dehydrogenase, cytosolic), or a protein having the amino acid sequence of SEQ ID NO: 447 (uncharacterized protein LOC100782924), or causes allergy to soybean.

[0191] (34α) Spot 34α antigen As a result of sequence identification by mass spectrometry for spot 34α, the following amino acid sequence was detected. LFLEEIARDIQK (sequence number 454) LFLEEIARDIQK (sequence number 457) TTFDAVIHFAGLK (SEQ ID NO: 458) In addition, for spot 34α, mass data obtained from a mass spectrometer was analyzed by collating it with NCBI protein data, and bifunctional UDP-glucose 4-epimerase and UDP-xylose 4-epimerase 1-like (amino acid sequence: SEQ ID NO: 453, base sequence encoding it: SEQ ID NO: 452) was identified as a protein containing SEQ ID NO: 454, and uncharacterized protein LOC100803483 (amino acid sequence: SEQ ID NO: 456, base sequence encoding it: SEQ ID NO: 455) was identified as a protein containing either SEQ ID NO: 457 or 458.

[0192] Therefore, in the present application, the antigen of spot 34α may be any of the proteins selected from the group consisting of (34αA1-a) to (34αA1-e), (34αB1), (34αA2-a) to (34αA2-e), and (34αB2). (34αA1-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 453. (34αA1-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 453. (34αA1-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 452 have been deleted, substituted, inserted or added. (34αA1-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 452. (34αA1-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 452. (34αB1) A protein comprising the amino acid sequence represented by SEQ ID NO: 454. In this case, the amino acid sequence represented by SEQ ID NO: 464 may have one or several amino acids deleted, substituted, inserted or added. (34αA2-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 456. (34αA2-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 456. (34αA2-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 455 have been deleted, substituted, inserted or added. (34αA2-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 455. (34αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 455. (34αB2) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 457 and 458, preferably a protein comprising both of said amino acid sequences. Here, the amino acid sequence shown in either SEQ ID NO: 457 or 458 may have one or several amino acids deleted, substituted, inserted or added.

[0193] The proteins selected from the group consisting of (34αA1-a) to (34αA1-e), (34αB1), (34αA2-a) to (34αA2-e), and (34αB2) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, etc.

[0194] The protein selected from the group consisting of (34αA1-a) to (34αA1-e), (34αB1), (34αA2-a) to (34αA2-e), and (34αB2) may be a protein that appears as a spot with a molecular weight of 10 to 70 kDa, preferably a molecular weight of about 20 to 60 kDa, and an isoelectric point of 5.5 to 10.5, preferably 6.0 to 10.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the above section ``Identification of antigen.''

[0195] Preferably, the protein that is an antigen of spot 34α has activity equivalent to that of a protein having the amino acid sequence of SEQ ID NO: 453 (bifunctional UDP-glucose 4-epimerase and UDP-xylose 4-epimerase 1-like) or a protein having the amino acid sequence of SEQ ID NO: 456 (uncharacterized protein LOC100803483), or causes allergy to soybean.

[0196] (35α) Spot 35α antigen As a result of sequence identification by mass spectrometry for spot 35α, the following amino acid sequence was detected. ARYDEIVK (SEQ ID NO: 461) DGQTREHALLAFTLGVK (SEQ ID NO: 462) EVSSYLK (SEQ ID NO: 463) FSEILTK (sequence number 464) STTTGHLIYK (sequence number 465) THINIVVIGHVDSGK (SEQ ID NO: 466) DGQTREHALLAFTLGVK (sequence number 469) EVSSYLK (SEQ ID NO: 470) STTTGHLIYK (SEQ ID NO: 471) ARYDEIVK (SEQ ID NO: 474) DGQTREHALLSFTLGVK (sequence number 475) EVSSYLK (SEQ ID NO: 476) STTTGHLIYK (sequence number 477) In addition, the mass data obtained from the mass spectrometer for spot 35α was analyzed by collating it with the protein data of NCBI, and it was found to be a protein containing any one of SEQ ID NOs: 461 to 466, which is elongation factor 1-alpha (elongation factor 1-alpha ) (amino acid sequence: SEQ ID NO: 460, base sequence encoding same: SEQ ID NO: 459), elongation factor 1-alpha (amino acid sequence: SEQ ID NO: 468, base sequence encoding same: SEQ ID NO: 467) as a protein containing any one of SEQ ID NOs: 469 to 471, elongation factor-1A isoform X1 (amino acid sequence: SEQ ID NO: 474 to 477) as a protein containing any one of SEQ ID NOs: 474 to 477 The sequence encoding it (SEQ ID NO: 472) was identified.

[0197] Therefore, in the present application, the antigen of spot 35α is selected from the group consisting of (35αA1-a) to (35αA1-e), (35αB1), (35αA2-a) to (35αA2-e), (35αB2), (35αA3-a) to (35αA3-e), and (35αB3). It may be any protein of choice. (35αA1-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 460. (35αA1-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 460. (35αA1-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides in SEQ ID NO: 459 have been deleted, substituted, inserted or added. (35αA1-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 459. (35αA1-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 459. (35αB1) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 461 to 466, preferably at least two, three, four, five, or all of the amino acid sequences, wherein the amino acid sequence shown in any of SEQ ID NOs: 461 to 466 may have one or several amino acids deleted, substituted, inserted, or added. (35αA2-a) A protein comprising an amino acid sequence in which one or more amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 468. (35αA2-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 468. (35αA2-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 467 have been deleted, substituted, inserted or added. (35αA2-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 467. (35αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 467. (35αB2) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 469 to 471, preferably at least two or all of the amino acid sequences, wherein the amino acid sequence shown in any of SEQ ID NOs: 469 to 471 may have one or several amino acids deleted, substituted, inserted or added. (35αA3-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 473. (35αA3-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 473. (35αA3-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 472 have been deleted, substituted, inserted or added. (35αA3-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 472. (35αA3-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 472. (35αB3) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 474 to 477, preferably at least two, three, or all of the amino acid sequences, wherein the amino acid sequence shown in any of SEQ ID NOs: 474 to 477 may have one or several amino acids deleted, substituted, inserted, or added.

[0198] The proteins selected from the group consisting of (35αA1-a) to (35αA1-e), (35αB1), (35αA2-a) to (35αA2-e), (35αB2), (35αA3-a) to (35αA3-e), and (35αB3) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, etc.

[0199] The protein selected from the group consisting of (35αA1-a) to (35αA1-e), (35αB1), (35αA2-a) to (35αA2-e), (35αB2), (35αA3-a) to (35αA3-e), and (35αB3) may be a protein that appears as a spot with a molecular weight of 20 to 90 kDa, preferably a molecular weight of about 30 to 80 kDa, and an isoelectric point of 5.5 to 10.5, preferably 6.0 to 10.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the above section ``Identification of antigen.''

[0200] Preferably, the protein that is the antigen of spot 35α is a protein having the amino acid sequence of SEQ ID NO: 460 or 468 (elongation factor 1-alpha) or SEQ ID NO: 4 Equivalent to a protein with a sequence of 73 amino acids (elongation factor-1A isoform X1) It has activity or causes allergy to soy.

[0201] (36α, 37α) Spot 36α, 37α antigen spot 36α、37α As a result of sequence identification by mass spectrometry, the following amino acid sequence was detected. EGSSIINTTSVNAYK (sequence number 480) FPPQQQQTQPGK (SEQ ID NO: 481) GAIVAYTRGLALQLVSK (SEQ ID NO: 482) LLDYTSTK (SEQ ID NO: 483) EGATVAFTYVK (sequence number 486) QVIDLVVK (sequence number 487) EGSSIINTTSVNAYK (sequence number 490) FPPQQQQTQPGK (SEQ ID NO: 491) GAIVAYTRGLALQLVSK (SEQ ID NO: 492) LLDYTSTK (SEQ ID NO: 493) In addition, for spots 36α and 37α, the mass data obtained from the mass spectrometer was analyzed by collating it with the protein data of NCBI, and the following proteins were identified: seed maturation protein PM34 (amino acid sequence: SEQ ID NO: 479, base sequence encoding same: SEQ ID NO: 478) as a protein containing any of SEQ ID NOs: 480 to 483; uncharacterized protein LOC100809384 (amino acid sequence: SEQ ID NO: 485, base sequence encoding same: SEQ ID NO: 484) as a protein containing any of SEQ ID NOs: 486 and 487; hypothetical protein GLYMA_10G155300 as a protein containing any of SEQ ID NOs: 449 to 493; (hypothetical protein GLYMA_10G155300) (amino acid sequence: SEQ ID NO: 489, base sequence encoding same: SEQ ID NO: 488) was identified.

[0202] Therefore, in the present application, the antigens of spots 36α and 37α are referred to as (36,37αA1-a) to (36,37αA1-e), (36,37αB1), and (36,37αA2-a) as follows: It may be any of the proteins selected from the group consisting of (36,37αA2-e), (36,37αB2), (36,37αA3-a) to (36,37αA3-e), and (36,37αB3). (36,37αA1-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 479. (36,37αA1-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 479. (36,37αA1-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 478 have been deleted, substituted, inserted or added. (36,37αA1-d) A protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the nucleotide sequence shown in SEQ ID NO: 478. (36,37αA1-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 478. (36,37αB1) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 480 to 483, preferably at least two, three, or all of the amino acid sequences, wherein the amino acid sequence shown in any of SEQ ID NOs: 480 to 483 may have one or several amino acids deleted, substituted, inserted, or added. (36,37αA2-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 485. (36,37αA2-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 485. (36,37αA2-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 484 have been deleted, substituted, inserted or added. (36,37αA2-d) A protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the nucleotide sequence shown in SEQ ID NO: 484. (36,37αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 484. (36,37αB2) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 486 and 487, preferably comprising both of said amino acid sequences. In this regard, the amino acid sequence shown in either SEQ ID NO: 486 or 487 may have one or several amino acids deleted, substituted, inserted or added. (36,37αA3-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 489. (36,37αA3-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 489. (36,37αA3-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 488 have been deleted, substituted, inserted or added. (36,37αA3-d) A protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 488. (36,37αA3-e) An amino acid encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 488. A protein that contains an amino acid sequence. (36,37αB3) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 490 to 493, preferably a protein comprising at least two, three, or all of said amino acid sequences, wherein the amino acid sequence shown in any of SEQ ID NOs: 490 to 493 may have one or several amino acids deleted, substituted, inserted, or added.

[0203] The proteins selected from the group consisting of the above (36,37αA1-a) to (36,37αA1-e), (36,37αB1), (36,37αA2-a) to (36,37αA2-e), (36,37αB2), (36,37αA3-a) to (36,37αA3-e), and (36,37αB3) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, etc.

[0204] The protein selected from the group consisting of the above (36,37αA1-a) to (36,37αA1-e), (36,37αB1), (36,37αA2-a) to (36,37αA2-e), (36,37αB2), (36,37αA3-a) to (36,37αA3-e), and (36,37αB3) may be a protein that appears as a spot with a molecular weight of 10 to 70 kDa, preferably a molecular weight of about 20 to 60 kDa, and an isoelectric point of 3.5 to 8.5, preferably 4.0 to 8.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the above section "Identification of antigen."

[0205] Preferably, the protein that is an antigen of spots 36α and 37α has activity equivalent to that of a protein having the amino acid sequence of SEQ ID NO: 479 (seed maturation protein PM34), a protein having the amino acid sequence of SEQ ID NO: 485 (uncharacterized protein LOC100809384) or a protein having the amino acid sequence of SEQ ID NO: 489 (hypothetical protein GLYMA_10G155300), or causes allergy to soybeans.

[0206] (38α) Spot 38α antigen As a result of sequence identification by mass spectrometry for spot 38α, the following amino acid sequence was detected. AAGSTTAQYVGEK (sequence number 496) ALATGWSAAHYSTEITVEGTK (SEQ ID NO: 497) AQVAGSTTVQYAGEK (sequence number 498) DTIGRVDNITTPLAK (SEQ ID NO: 499) DTIGSVDHITTPLAEK (SEQ ID NO: 500) ERELASDRARSGNIVK (SEQ ID NO:501) ERYERANQATSEAAK (SEQ ID NO:502) GGGEEQGRRREEIGEENK (SEQ ID NO: 503) GLAAAAAGENAK (SEQ ID NO:504) GRVVIESEK (sequence number 505) GVAEYAGQK (SEQ ID NO:506) LGSAAHTLVK (SEQ ID NO:507) QSSLEEIFK (SEQ ID NO:508) QSSLEEISK (sequence number 509) REQKPLGSIIGESFQGVQEK (SEQ ID NO:510) TGNETQDIGQK (sequence number 511) VIVAGEGEK (SEQ ID NO:512) VQQHQGEGGGGVLNAIGETIAEIAETTK (SEQ ID NO: 513) In addition, the mass data obtained from the mass spectrometer for spot 38α was uploaded to the NCBI database. Analysis of the protein data revealed that the protein contained any one of SEQ ID NOs: 496 to 513, and thus identified it as seed biotin-containing protein SBP65-like isoform X1 (amino acid sequence: SEQ ID NO: 495, base sequence encoding same: SEQ ID NO: 494).

[0207] Therefore, in the present application, the antigen of spot 38α may be any protein selected from the group consisting of (38αA-a) to (38αA-e) and (38αB) below. (38αA-a) A protein comprising an amino acid sequence in which one or more amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 495. (38αA-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 495. (38αA-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides in SEQ ID NO: 494 have been deleted, substituted, inserted or added. (38αA-d) A protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 494. (38αA-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 494. (38αB) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 496 to 513, preferably at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, or all of the amino acid sequences, wherein one or several amino acids may be deleted, substituted, inserted, or added in the amino acid sequence shown in any of SEQ ID NOs: 496 to 513.

[0208] Proteins selected from the group consisting of (38αA-a) to (38αA-e) and (38αB) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, etc.

[0209] The protein selected from the group consisting of (38αA-a) to (38αA-e) and (38αB) may be a protein that appears as a spot with a molecular weight of 70 to 170 kDa, preferably a molecular weight of about 80 to 160 kDa, and an isoelectric point of 5.5 to 10.5, preferably 6.0 to 10.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above.

[0210] Preferably, the protein that is an antigen of spot 38α has activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 495 (seed biotin-containing protein SBP65-like isoform X1) or causes allergy to soybean.

[0211] (39α) Spot 39α antigen As a result of sequence identification by mass spectrometry for spot 39α, the following amino acid sequence was detected. ETLQEIIGSGAK (SEQ ID NO:516) In addition, for spot 39α, the mass data obtained from the mass spectrometer was analyzed by collating it with the protein data of NCBI, and it was found that the protein containing SEQ ID NO: 516 is ATP synthase subunit O, mitochondrial-like (amino acid sequence: SEQ ID NO: 515, the base sequence encoding it: SEQ ID NO: 516). No. 514) was identified.

[0212] Therefore, in the present application, the antigen of spot 39α may be any protein selected from the group consisting of (39αA-a) to (39αA-e) and (39αB) below. (39αA-a) A protein comprising an amino acid sequence in which one or more amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 515. (39αA-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 515. (39αA-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides in SEQ ID NO: 514 have been deleted, substituted, inserted or added. (39αA-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 514. (39αA-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 514. (39αB) A protein comprising the amino acid sequence shown in SEQ ID NO: 516. In this case, the amino acid sequence shown in SEQ ID NO: 516 may have one or several amino acids deleted, substituted, inserted or added.

[0213] Proteins selected from the group consisting of (39αA-a) to (39αA-e) and (39αB) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, etc.

[0214] The protein selected from the group consisting of (39αA-a) to (39αA-e) and (39αB) may be a protein that appears as a spot with a molecular weight of 5 to 60 kDa, preferably a molecular weight of about 10 to 50 kDa, and an isoelectric point of 5.5 to 10.5, preferably 6.0 to 10.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the above section "Identification of antigen."

[0215] Preferably, the protein that is an antigen of spot 39α has activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 515 (ATP synthase subunit O, mitochondrial-like), or causes allergy to soybean.

[0216] (40α, 56α) Spot 40α, 56α antigens As a result of sequence identification by mass spectrometry for spots 40α and 56α, the following amino acid sequences were detected. EVGQDIQSK (SEQ ID NO:519) RTTVTATTATA (SEQ ID NO:520) In addition, for spots 40α and 56α, the mass data obtained from a mass spectrometer was analyzed by collating it with the protein data of NCBI, and it was identified as P24 oleosin isoform A (P24 oleosin isoform A) as a protein containing either SEQ ID NO: 519 or 520. ) (amino acid sequence: SEQ ID NO:518, base sequence encoding same: SEQ ID NO:517) was identified.

[0217] Therefore, in the present application, the antigens of spots 40α and 56α may be any of the proteins selected from the group consisting of (40,56αA-a) to (40,56αA-e) and (40,56αB) below. (40,56αA-a) A protein comprising an amino acid sequence in which one or more amino acids have been deleted, substituted, inserted or added in SEQ ID NO:518. (40,56αA-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 518. (40,56αA-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides in SEQ ID NO:517 have been deleted, substituted, inserted or added. (40,56αA-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 517. (40,56αA-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO:517. (40,56αB) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 519 and 520, preferably comprising both of said amino acid sequences. In this regard, the amino acid sequence shown in either SEQ ID NO: 519 or 520 may have one or several amino acids deleted, substituted, inserted or added.

[0218] Proteins selected from the group consisting of (40,56αA-a) to (40,56αA-e) and (40,56αB) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, etc.

[0219] The protein selected from the group consisting of (40,56αA-a) to (40,56αA-e) and (40,56αB) may be a protein that appears as a spot with a molecular weight of 5 to 60 kDa, preferably a molecular weight of about 10 to 50 kDa, and an isoelectric point of 5.5 to 10.5, preferably 6.0 to 10.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the above section "Identification of antigen."

[0220] Preferably, the protein that is an antigen of spot 40α or 56α has an activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 518 (P24 oleosin isoform A), or , which can cause allergies to soybeans.

[0221] (41α) Spot 41α antigen As a result of sequence identification by mass spectrometry for spot 41α, the following amino acid sequence was detected. NAYGGSMK (SEQ ID NO:523) In addition, when mass data obtained from a mass spectrometer for spot 41α was analyzed by comparing it with NCBI protein data, the protein containing sequence number 523 was identified as uncharacterized protein At3g49720-like isoform X2 (amino acid sequence: sequence number 522, base sequence encoding same: sequence number 521).

[0222] Therefore, in the present application, the antigen of spot 41α may be any protein selected from the group consisting of (41αA-a) to (41αA-e) and (41αB) below. (41αA-a) A protein comprising an amino acid sequence in which one or more amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 522. (41αA-b) The amino acid sequence shown in SEQ ID NO: 522 has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, or 98% or more. % or more, or 99% or more, of the amino acid sequence. (41αA-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 521 have been deleted, substituted, inserted or added. (41αA-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 521. (41αA-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO:521. (41αB) A protein comprising the amino acid sequence shown in SEQ ID NO: 523. In this case, the amino acid sequence shown in SEQ ID NO: 523 may have one or several amino acids deleted, substituted, inserted or added.

[0223] Proteins selected from the group consisting of (41αA-a) to (41αA-e) and (41αB) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, etc.

[0224] The protein selected from the group consisting of (41αA-a) to (41αA-e) and (41αB) above may be a protein that appears as a spot with a molecular weight of 5 to 60 kDa, preferably a molecular weight of about 10 to 50 kDa, and an isoelectric point of 5.5 to 10.5, preferably 6.0 to 10.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the above section "Identification of antigen."

[0225] Preferably, the protein that is an antigen of spot 41α has activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 515 (ATP synthase subunit O, mitochondrial-like), or causes allergy to soybean.

[0226] (42α, 43α) Spot 42α, 43α antigens As a result of sequence identification by mass spectrometry for spots 42α and 43α, the following amino acid sequences were detected. ALEQLQDAIAK (SEQ ID NO:526) HHQTYITNYNK (SEQ ID NO:527) NLAPVREGGGEPPK (SEQ ID NO:528) NVRPDYLK (SEQ ID NO:529) RLVVETTANQDPLVTK (SEQ ID NO:530) YASEVYEK (SEQ ID NO:531) ALEQLQDAVAK (SEQ ID NO:534) HHQTYITNFNK (SEQ ID NO:535) NLAPVREGGGEPPK (SEQ ID NO:536) NVRPDYLK (SEQ ID NO:537) RLVVETTANQDPLVTK (SEQ ID NO:538) YASEVYEK (SEQ ID NO:539) In addition, for spots 42α and 43α, the mass data obtained from a mass spectrometer was analyzed by collating it with the protein data of NCBI, and it was found that superoxide dismutase [Mn], mitochondrial (amino acid sequence: SEQ ID NO: 525, base sequence encoding same: SEQ ID NO: 524) was identified as a protein containing any of SEQ ID NOs: 526 to 531, and hypothetical protein GLYMA_04G221300 (amino acid sequence: SEQ ID NO: 533, base sequence encoding same: SEQ ID NO: 534 to 539) was identified as a protein containing any of SEQ ID NOs: 534 to 539. 532) was identified.

[0227] Therefore, in the present application, the antigens of spots 42α and 43α may be any of the proteins selected from the group consisting of (42,43αA1-a) to (42,43αA1-e), (42,43αB1), (42,43αA2-a) to (42,43αA2-e), and (42,43αB2). (42,43αA1-a) A protein comprising an amino acid sequence in which one or more amino acids have been deleted, substituted, inserted or added in SEQ ID NO:525. (42,43αA1-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 525. (42,43αA1-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO:524 have been deleted, substituted, inserted or added. (42,43αA1-d) A protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the nucleotide sequence shown in SEQ ID NO: 524. (42,43αA1-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 524. (42, 43αB1) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 526 to 531, preferably at least two, three, four, five, or all of the amino acid sequences, wherein the amino acid sequence shown in any of SEQ ID NOs: 526 to 531 may have one or several amino acids deleted, substituted, inserted, or added. (42,43αA2-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO:533. (42,43αA2-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 533. (42,43αA2-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO:532 have been deleted, substituted, inserted or added. (42,43αA2-d) A protein comprising an amino acid sequence encoded by a nucleotide sequence having an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the nucleotide sequence shown in SEQ ID NO: 532. (42,43αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 532. (42, 43αB2) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 534 to 539, preferably at least 2, 3, 4, 5, or all of the amino acid sequences, wherein the amino acid sequence shown in any of SEQ ID NOs: 534 to 539 may have one or several amino acids deleted, substituted, inserted, or added.

[0228] The proteins selected from the group consisting of (42,43αA1-a) to (42,43αA1-e), (42,43αB1), (42,43αA2-a) to (42,43αA2-e), and (42,43αB2) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, or the like.

[0229] From the above (42,43αA1-a) to (42,43αA1-e), (42,43αB1), (42,43αA2-a) to (42,43αA2-e), and (42,43αB2), The protein selected from the group consisting of may be a protein that appears as a spot having a molecular weight of about 5 to 60 kDa, preferably a molecular weight of about 10 to 50 kDa, and an isoelectric point of 5.5 to 10.5, preferably 6.0 to 10.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the above section "Identification of antigen."

[0230] Preferably, the protein that is an antigen of spots 42α and 43α has activity equivalent to that of a protein having the amino acid sequence of SEQ ID NO: 525 (superoxide dismutase [Mn], mitochondrial) or a protein having the amino acid sequence of SEQ ID NO: 533 (hypothetical protein GLYMA_04G221300), or causes allergy to soybean.

[0231] (45α) spot 45α Antigens As a result of sequence identification by mass spectrometry for spot 45α, the following amino acid sequence was detected. HVPGFIERAEELK (SEQ ID NO:542) RFALLVEDLK (SEQ ID NO:543) VANVESGGEFTISSAEEIIK (SEQ ID NO:544) YTNALGLELDLTDK (SEQ ID NO:545) HVPGFIERAEELK (SEQ ID NO:548) RFALLVEDLK (SEQ ID NO:549) VANVESGGEFTISSAEEIIK (SEQ ID NO: 550) YTNALGLELDLTDK (SEQ ID NO:551) In addition, for spot 45α, the mass data obtained from the mass spectrometer was analyzed by collating it with the protein data of NCBI, and it was found that the protein containing any of SEQ ID NOs: 542 to 545 is uncharacterized protein LOC100499771 (amino acid sequence: SEQ ID NO: 541, base sequence encoding the same: SEQ ID NO: 540), and the protein containing any of SEQ ID NOs: 548 to 551 is hypothetical protein GLYMA_09G192800 (amino acid sequence: SEQ ID NO: 548 to 551). The sequence: SEQ ID NO: 547, and the base sequence encoding it: SEQ ID NO: 546) were identified.

[0232] Therefore, in the present application, the antigen of spot 45α may be any of the proteins selected from the group consisting of (45αA1-a) to (45αA1-e), (45αB1), (45αA2-a) to (45αA2-e), and (45αB2). (45αA1-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 541. (45αA1-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 541. (45αA1-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides in SEQ ID NO: 540 have been deleted, substituted, inserted or added. (45αA1-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 540. (45αA1-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO:540. (45αB1) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 542 to 545, preferably at least two, three, or all of the amino acid sequences, wherein the amino acid sequence shown in any of SEQ ID NOs: 542 to 545 may have one or several amino acids deleted, substituted, inserted, or added. (45αA2-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO:547. (45αA2-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 547. (45αA2-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides in SEQ ID NO: 546 have been deleted, substituted, inserted or added. (45αA2-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 546. (45αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO:546. (45αB2) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 548 to 551, preferably at least two, three, or all of the amino acid sequences, wherein the amino acid sequence shown in any of SEQ ID NOs: 548 to 551 may have one or several amino acids deleted, substituted, inserted, or added.

[0233] The proteins selected from the group consisting of (45αA1-a) to (45αA1-e), (45αB1), (45αA2-a) to (45αA2-e), and (45αB2) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, etc.

[0234] The protein selected from the group consisting of (45αA1-a) to (45αA1-e), (45αB1), (45αA2-a) to (45αA2-e), and (45αB2) may be a protein that appears as a spot with a molecular weight of 2 to 50 kDa, preferably a molecular weight of about 5 to 40 kDa, and an isoelectric point of 3.5 to 7.5, preferably 4.0 to 7.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the above section ``Identification of antigen.''

[0235] Preferably, the protein that is an antigen of spot 45α has activity equivalent to that of a protein having the amino acid sequence of SEQ ID NO: 541 (uncharacterized protein LOC100499771) or a protein having the amino acid sequence of SEQ ID NO: 547 (hypothetical protein GLYMA_09G192800), or causes allergy to soybean.

[0236] (46α) Spot 46α antigen As a result of sequence identification by mass spectrometry for spot 46α, the following amino acid sequence was detected. DNVEYLAK (SEQ ID NO:554) In addition, when the mass data obtained from a mass spectrometer for spot 46α was analyzed by comparing it with NCBI protein data, seed maturation protein PM22 (amino acid sequence: sequence number 553, base sequence encoding it: sequence number 552) was identified as a protein containing sequence number 554.

[0237] Therefore, in the present application, the antigen of spot 46α may be any of the proteins selected from the group consisting of (46αA-a) to (46αA-e) and (46αB) below. (46αA-a) A protein comprising an amino acid sequence in which one or more amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 553. (46αA-b) 70% or more identity to the amino acid sequence shown in SEQ ID NO: 553, preferably A protein that contains 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more of the amino acid sequence. (46αA-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides in SEQ ID NO: 552 have been deleted, substituted, inserted or added. (46αA-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 552. (46αA-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 552. (46αB) A protein comprising the amino acid sequence shown in SEQ ID NO: 554. In this case, the amino acid sequence shown in SEQ ID NO: 554 may have one or several amino acids deleted, substituted, inserted or added.

[0238] Proteins selected from the group consisting of (46αA-a) to (46αA-e) and (46αB) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, etc.

[0239] The protein selected from the group consisting of (46αA-a) to (46αA-e) and (46αB) above may be a protein that appears as a spot with a molecular weight of 2 to 50 kDa, preferably a molecular weight of about 5 to 40 kDa, and an isoelectric point of 3.5 to 7.5, preferably 4.0 to 7.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the "Identification of Antigen" section above.

[0240] Preferably, the protein that is an antigen of spot 46α has activity equivalent to that of the protein having the amino acid sequence of SEQ ID NO: 553 (seed maturation protein PM22), or causes allergy to soybean.

[0241] (47α) Spot 47α antigen As a result of sequence identification by mass spectrometry for spot 47α, the following amino acid sequence was detected. LPTDESLLTQIK (SEQ ID NO:557) NGYIVIK (SEQ ID NO:558) NGYIVIK (SEQ ID NO:561) TYPQQAGTIRK (SEQ ID NO:562) VVEVSTSK (SEQ ID NO:563) In addition, the mass data obtained from the mass spectrometer for spot 47α was analyzed by collating it with the protein data of NCBI, and it was found to be eukaryotic translation initiation factor 5A-2 (amino acid sequence: SEQ ID NO: 5) as a protein containing either SEQ ID NO: 557 or 558. Uncharacterized protein LOC100305510 (amino acid sequence: SEQ ID NO: 560, base sequence encoding it: SEQ ID NO: 559) was identified as a protein containing any of SEQ ID NOs: 561 to 563.

[0242] Therefore, in the present application, the antigen of spot 47α may be any of the proteins selected from the group consisting of (47αA1-a) to (47αA1-e), (47αB1), (47αA2-a) to (47αA2-e), and (47αB2). (47αA1-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 556. (47αA1-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 556. (47αA1-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides in SEQ ID NO: 555 have been deleted, substituted, inserted or added. (47αA1-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 555. (47αA1-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO:555. (47αB1) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 557 and 558, preferably comprising both of said amino acid sequences. In this regard, the amino acid sequence shown in either SEQ ID NO: 557 or 558 may have one or several amino acids deleted, substituted, inserted or added. (47αA2-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO:560. (47αA2-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 560. (47αA2-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides in SEQ ID NO: 559 have been deleted, substituted, inserted or added. (47αA2-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 559. (47αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO:559. (47αB2) A protein comprising at least one of the amino acid sequences selected from the group consisting of SEQ ID NOs: 561 to 563, preferably at least two or all of the amino acid sequences, wherein the amino acid sequence shown in any of SEQ ID NOs: 561 to 563 may have one or several amino acids deleted, substituted, inserted or added.

[0243] The proteins selected from the group consisting of (47αA1-a) to (47αA1-e), (47αB1), (47αA2-a) to (47αA2-e), and (47αB2) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, etc.

[0244] The protein selected from the group consisting of (47αA1-a) to (47αA1-e), (47αB1), (47αA2-a) to (47αA2-e), and (47αB2) may be a protein that appears as a spot with a molecular weight of 2 to 50 kDa, preferably a molecular weight of about 5 to 40 kDa, and an isoelectric point of 3.5 to 7.5, preferably 4.0 to 7.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the above section ``Identification of antigen.''

[0245] Preferably, the protein that is the antigen of spot 47α is a protein having the amino acid sequence of SEQ ID NO: 556 (eukaryotic translation initiation factor 5A-2) or a sequence thereof. It has activity equivalent to that of the protein having the amino acid sequence of sequence number 560 (uncharacterized protein LOC100305510), or causes allergy to soybeans.

[0246] (48α) Spot 48α antigen As a result of sequence identification by mass spectrometry for spot 48α, the following amino acid sequence was detected. AEFPIFDK (sequence number 566) DARGNNVNLADYK (SEQ ID NO:567) DGNVVDRYAPTTSPLSIEK (SEQ ID NO: 568) GGLFGDSIK (SEQ ID NO:569) VDVNGDNAAPLYK (SEQ ID NO:570) In addition, when the mass data obtained from a mass spectrometer for spot 48α was analyzed by comparing it with NCBI protein data, uncharacterized protein LOC100306570 (amino acid sequence: sequence number 565, base sequence encoding it: sequence number 564) was identified as a protein containing any of sequence numbers 566 to 570.

[0247] Therefore, in the present application, the antigen of spot 48α may be any of the proteins selected from the group consisting of (48αA-a) to (48αA-e) and (48αB) below. (48αA-a) A protein comprising an amino acid sequence in which one or more amino acids have been deleted, substituted, inserted or added in SEQ ID NO: 565. (48αA-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 565. (48αA-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides in SEQ ID NO: 564 have been deleted, substituted, inserted or added. (48αA-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown in SEQ ID NO: 564. (48αA-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 564. (48αB) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 566 to 570, preferably at least two, three, four, or all of said amino acid sequences, wherein one or several amino acids may be deleted, substituted, inserted, or added in the amino acid sequence shown in any of SEQ ID NOs: 566 to 570.

[0248] Proteins selected from the group consisting of (48αA-a) to (48αA-e) and (48αB) also include proteins whose amino acid residues have been modified by phosphorylation, glycosylation, aminoacylation, ring-opening, deamination, etc.

[0249] The protein selected from the group consisting of (48αA-a) to (48αA-e) and (48αB) may be a protein that appears as a spot with a molecular weight of 2 to 50 kDa, preferably a molecular weight of about 5 to 40 kDa, and an isoelectric point of 3.5 to 8.5, preferably 4.0 to 8.0, in a gel when two-dimensional electrophoresis is performed under the conditions described in the above section "Identification of antigen."

[0250] Preferably, the protein that is an antigen of spot 48α has activity equivalent to the protein having the amino acid sequence of SEQ ID NO: 565 (uncharacterized protein LOC100306570) or causes allergy to soybean.

[0251] (50α) Spot 50α antigen As a result of sequence identification by mass spectrometry for spot 50α, the following amino acid sequence was detected. AADYLHNYQGDNTTAPSQPAESK (SEQ ID NO:573) LVAEAAQSALK (SEQ ID NO:574) QGIGQYVDK (sequence number 575) VADAAGDLLDAAGK (SEQ ID NO:576) In addition, when the mass data obtained from a mass spectrometer for spot 50α was analyzed by comparing it with NCBI protein data, uncharacterized protein LOC100813859 (amino acid sequence: sequence number 572, base sequence encoding it: sequence number 571) was identified as a protein containing any of sequence numbers 573 to 576.

[0252] Therefore, in the present application, the antigen of spot 50α may be any protein selected from the group consisting of (50αA-a) to (50αA-e) and (50αB) below. (50αA-a) A protein comprising an amino acid sequence in which one or several amino acids have been deleted, substituted, inserted or added in SEQ ID NO:572. (50αA-b) A protein having an amino acid sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the amino acid sequence shown in SEQ ID NO: 572. (50αA-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or more nucleotides in SEQ ID NO: 571 have been deleted, substituted, inserted or added. (50αA-d) A protein comprising an amino acid sequence encoded by a base sequence that has an identity of 70% or more, preferably 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 97% or more, 98% or more, or 99% or more to the base sequence shown ...

Claims

1. A diagnostic kit for soybean allergy, comprising at least one of the following proteins (1) to (8): (1) (1A) Bowman-Birk type proteinase inhibitor D-II or a mutant thereof, comprising the following (1A-a) to (1A-b): Any of the proteins of (1A-e): (1A-a) One or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 2 a protein comprising the selected amino acid sequence; (1A-b) An amino acid sequence having an identity of 70% or more with the amino acid sequence shown in SEQ ID NO:2 A protein comprising: (1A-c) SEQ ID NO: 1 in which one or several nucleotides are deleted, substituted, inserted or added a protein comprising an amino acid sequence encoded by the added base sequence; (1A-d) A nucleic acid sequence having an identity of 70% or more with the nucleic acid sequence shown in SEQ ID NO:

1. a protein comprising the amino acid sequence to be encoded; or (1A-e) A nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 1 and a sequence a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions; or (1B) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 17 to 20; (2) (2A) Ribulose bisphosphate carboxylase large chain bisphosphate carboxylase large chain) or a variant thereof, which is any one of the following proteins (2A-a) to (2A-e): (2A-a) One or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 4 a protein comprising the selected amino acid sequence; (2A-b) An amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO: 4 A protein comprising: (2A-c) SEQ ID NO: 3 in which one or several nucleotides are deleted, substituted, inserted or added a protein comprising an amino acid sequence encoded by the added base sequence; (2A-d) A nucleic acid sequence having an identity of 70% or more with the nucleic acid sequence shown in SEQ ID NO:

3. a protein comprising the amino acid sequence to be encoded; or (2A-e) A nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 3 and a sequence a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions; or (2B) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 21 to 28; (3) (3A) Gamma-glutamyl hydrolase or a mutant thereof, which is any one of the following proteins (3A-a) to (3A-e): (3A-a) SEQ ID NO: 6 in which one or several amino acids are deleted, substituted, inserted or added a protein comprising the selected amino acid sequence; (3A-b) An amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO:6 A protein comprising: (3A-c) SEQ ID NO: 5 in which one or several nucleotides are deleted, substituted, inserted or added a protein comprising an amino acid sequence encoded by the added base sequence; (3A-d) A clone having a base sequence having an identity of 70% or more with the base sequence shown in SEQ ID NO:

5. a protein comprising the amino acid sequence to be encoded; or (3A-e) A nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO:

5. a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions; or (3B) a protein comprising the amino acid sequence represented by SEQ ID NO: 29 or 30; (4) (4A) Guanine nucleotide-binding protein subunit beta-like protein or a mutant thereof, which is any one of the following proteins (4A-a) to (4A-e): (4A-a) One or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 8 a protein comprising the selected amino acid sequence; (4A-b) An amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO:8 A protein comprising: (4A-c) SEQ ID NO: 7 in which one or several nucleotides are deleted, substituted, inserted or added a protein comprising an amino acid sequence encoded by the added base sequence; (4A-d) A clone having a base sequence having 70% or more identity with the base sequence shown in SEQ ID NO:

7. a protein comprising the amino acid sequence to be encoded; or (4A-e) A nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 7 and a sequence a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions; or (4B) a protein comprising the amino acid sequence represented by SEQ ID NO: 31 or 32; (5) (5A) Protein SLE3 or a mutant thereof, which is any one of the following proteins (5A-a) to (5A-e): (5A-a) One or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 10 a protein comprising the determined amino acid sequence; (5A-b) An amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO: 10 Proteins containing strings; (5A-c) SEQ ID NO: 9 in which one or several nucleotides are deleted, substituted, inserted or added a protein comprising an amino acid sequence encoded by the added base sequence; (5A-d) A clone having a base sequence having an identity of 70% or more with the base sequence shown in SEQ ID NO:

9. a protein comprising the amino acid sequence to be encoded; or (5A-e) A nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 9 and a sequence a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions; or (5B) a protein comprising the amino acid sequence represented by SEQ ID NO: 33 or 34; (6) (6A) Seed biotin-containing protein SBP65 or a mutant thereof, which is any one of the following proteins (6A-a) to (6A-e): (6A-a) One or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 12 a protein comprising the determined amino acid sequence; (6A-b) An amino acid sequence having an identity of 70% or more with the amino acid sequence shown in SEQ ID NO: 12 Proteins containing strings; (6A-c) SEQ ID NO: 11 in which one or several nucleotides are deleted, substituted, inserted or A protein comprising an amino acid sequence encoded by the added base sequence; (6A-d) A base sequence having an identity of 70% or more with the base sequence shown in SEQ ID NO: 11 A protein comprising the encoded amino acid sequence; or (6A-e) A nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO:

11. a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions; or (6B) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 35 to 53; (7) (7A) Ferritin-2 or a mutant thereof, comprising the following (7A- Any of the proteins a) to (7A-e): (7A-a) One or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 14 a protein comprising the determined amino acid sequence; (7A-b) An amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO: 14 Proteins containing strings; (7A-c) SEQ ID NO: 13 in which one or several nucleotides are deleted, substituted, inserted or A protein comprising an amino acid sequence encoded by the added base sequence; (7A-d) a base sequence having an identity of 70% or more with the base sequence shown in SEQ ID NO: 13 A protein comprising the encoded amino acid sequence; or (7A-e) A nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO:

13. a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions; or (7B) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 54 to 61. (8) (8A) Cell division cycle protein 48 homolog or a mutant thereof, which is any one of the following proteins (8A-a) to (8A-e): (8A-a) One or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 16 a protein comprising the determined amino acid sequence; (8A-b) An amino acid sequence having an identity of 70% or more with the amino acid sequence shown in SEQ ID NO: 16 Proteins containing strings; (8A-c) SEQ ID NO: 15 in which one or several nucleotides are deleted, substituted, inserted or A protein comprising an amino acid sequence encoded by the added base sequence; (8A-d) A base sequence having an identity of 70% or more with the base sequence shown in SEQ ID NO: 15 A protein comprising the encoded amino acid sequence; or (8A-e) A nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO:

15. a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions; or (8B) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 62 to 68; The diagnostic kit as described above, comprising as an antigen.

2. A diagnostic kit for soybean allergy, comprising at least one of the following proteins (1α) to (55α): (1α)(1αA1) endoplasmin homolog isoform X2 or a mutant thereof, which is any one of the following proteins (1αA1-a) to (1αA1-e): (1αA1-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 70; (1αA1-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO: 70; (1αA1-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO:69 have been deleted, substituted, inserted or added; (1αA1-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 69; or (1αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO:69; (1αB1) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 71 to 87; (1αA2) endoplasmin homolog isoform X2 or a mutant thereof, which is any one of the following proteins (1αA2-a) to (1αA2-e): (1αA2-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO:89; (1αA2-b) an amino acid sequence having 70% or more identity with the amino acid sequence shown in SEQ ID NO:89 a protein comprising a nucleotide sequence; (1αA2-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 88 have been deleted, substituted, inserted or added; (1αA2-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 88; or (1αA2-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 88; or (1αB2) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 90 to 104; (2α) (2αA1) heat shock cognate protein 80 or a mutant thereof, comprising: Any of proteins (2αA1-a) to (2αA1-e): (2αA1-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 106; (2αA1-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO: 106; (2αA1-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 105 have been deleted, substituted, inserted or added; (2αA1-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 105; or (2αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 105; (2αB1) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 107 to 120; (2αA2) heat shock protein 90-1 or a mutant thereof, comprising the following (2αA2- Any of proteins a) to (2αA2-e): (2αA2-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 122; (2αA2-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO: 122; (2αA2-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 121 have been deleted, substituted, inserted or added; (2αA2-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 121; or (2αA2-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 121; or (2αB2) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 123 to 137; (2αA3) hypothetical protein GLYMA_02G302500 or a mutant thereof, which is any one of the following proteins (2αA3-a) to (2αA3-e): (2αA3-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 139; (2αA3-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO: 139; (2αA3-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 138 have been deleted, substituted, inserted or added; (2αA3-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 138; or (2αA3-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 138; or (2αB3) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 140 to 153; (4α) (4αA) embryo-specific urease isoform X1 or a mutant thereof, Any of proteins (4αA-a) to (4αA-e) of: (4αA-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 155; (4αA-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO: 155; (4αA-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 154 have been deleted, substituted, inserted or added; (4αA-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 154; or (4αA-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 154; or (4αB) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 156 and 157; (5α) (5αA) alpha-1,4 glucan phosphorylase L isozyme, chloroplastic / amyloplastic or a mutant thereof, which is any one of the following proteins (5αA-a) to (5αA-e): (5αA-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 159; (5αA-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO: 159; (5αA-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 158 have been deleted, substituted, inserted or added; (5αA-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 158; or (5αA-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 158; or (5αB) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 160 and 161; (6α)(6αA1) aconitate hydratase 1 or a mutant thereof, Any of proteins 1-a) to (6αA1-e): (6αA1-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 163; (6αA1-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO: 163; (6αA1-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 162 have been deleted, substituted, inserted or added; (6αA1-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 162; or (6αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence represented by SEQ ID NO: 162; (6αB1) At least one amino acid sequence selected from the group consisting of SEQ ID NOs: 164 to 167 A protein that contains one or more of the following: (6αA2) aconitate hydratase 1 or a mutant thereof, comprising the following (6αA2-a): Any of the proteins from (6αA2-e) to (6αA2-e): (6αA2-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 169; (6αA2-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO: 169; (6αA2-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 168 have been deleted, substituted, inserted or added; (6αA2-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 168; or (6αA2-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 168; or (6αB2) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 170 to 172; (7α)(7αA) methionine synthase or a mutant thereof, ) to (7αA-e): (7αA-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 174; (7αA-b) a protein comprising an amino acid sequence having 70% or more identity to the amino acid sequence shown in SEQ ID NO: 174; (7αA-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 173 have been deleted, substituted, inserted or added; (7αA-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 173; or (7αA-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 173; or (7αB) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 175 to 187; (8α)(8αA) transketolase, chloroplastic or a mutant thereof, which is any one of the following proteins (8αA-a) to (8αA-e): (8αA-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 189; (8αA-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO: 189; (8αA-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 188 have been deleted, substituted, inserted or added; (8αA-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 188; or (8αA-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 188; or (8αB) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 190 to 199; (9α)(9αA)lysine-tRNA ligase, cytoplasmic-like isoform X2 or its mutant Any of the following proteins (9αA-a) to (9αA-e): (9αA-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 201; (9αA-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO: 201; (9αA-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 200 have been deleted, substituted, inserted or added; (9αA-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 200; or (9αA-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 200; or (9αB) a protein comprising the amino acid sequence represented by SEQ ID NO: 202; (10α) (10αA1) chaperonin CPN60-2, mitochondrial or a mutant thereof , any one of the following proteins (10αA1-a) to (10αA1-e): (10αA1-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 204; (10αA1-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO: 204; (10αA1-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 203 have been deleted, substituted, inserted or added; (10αA1-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 203; or (10αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence represented by SEQ ID NO: 203; (10αB1) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 205 to 218; (10αA2) chaperonin CPN60-2, mitochondrial-like isoform X1 or its mutant Any of the following proteins (10αA2-a) to (10αA2-e): (10αA2-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 220; (10αA2-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO: 220; (10αA2-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 219 have been deleted, substituted, inserted or added; (10αA2-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 219; or (10αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 219; or (10αB2) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 221 to 231; (11α) (11αA) polyadenylate-binding protein 8 isoform X3 or a mutant thereof, which is any one of the following proteins (11αA-a) to (11αA-e): (11αA-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 233; (11αA-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO: 233; (11αA-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 232 have been deleted, substituted, inserted or added; (11αA-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 232; or (11αA-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 232; or (11αB) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 234 to 237; (12α) (12αA) pyrophosphate-fructose 6-phosphate 1-phosphotransferase subunit alpha-like or a mutant thereof, which is any one of the following proteins (12αA-a) to (12αA-e): (12αA-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 239; (12αA-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 239; (12αA-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 238 have been deleted, substituted, inserted or added; (12αA-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 238; or (12αA-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 238; or (12αB) a protein comprising the amino acid sequence set forth in SEQ ID NO: 240; A (13α) (13αA1) protein disulfide isomerase-like protein precursor or a mutant thereof, comprising any one of the following proteins (13αA1-a) to (13αA1-e): (13αA1-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 242; (13αA1-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO: 242; (13αA1-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 241 have been deleted, substituted, inserted or added; (13αA1-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 241; or (13αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence represented by SEQ ID NO: 241; (13αB1) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 243 to 252; (13αA2) protein disulfide-isomerase or a mutant thereof, Any of the proteins αA2-a) to (13αA2-e): (13αA2-a) A protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 254; (13αA2-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 254; (13αA2-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 253 have been deleted, substituted, inserted or added; (13αA2-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 253; or (13αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 253; or (13αB2) At least one amino acid sequence selected from the group consisting of SEQ ID NOs: 255 to 278 Proteins containing at least one (14α) (14αA1) V-type proton ATPase subunit B 2 or a mutant thereof, which is any one of the following proteins (14αA1-a) to (14αA1-e): (14αA1-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 280; (14αA1-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence represented by SEQ ID NO: 280; (14αA1-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 279 have been deleted, substituted, inserted or added; (14αA1-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 279; or (14αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 279; (14αB1) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 281 to 284; (14αA2) V-type proton ATPase subunit B 2 or a mutant thereof, which is any one of the following proteins (14αA2-a) to (14αA2-e): (14αA2-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 286; (14αA2-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO: 286; (14αA2-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 285 have been deleted, substituted, inserted or added; (14αA2-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 285; or (14αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 285; or (14αB2) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 287 to 289; (15,16α) (15,16αA1)UTP-glucose-1-phosphate uridylyltransferase or a mutant thereof, which is any one of the following proteins (15,16αA1-a) to (15,16αA1-e): (15,16αA1-a) A protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 291; (15,16αA1-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO: 291; (15,16αA1-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 290 have been deleted, substituted, inserted or added; (15,16αA1-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 290; or (15,16αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence represented by SEQ ID NO: 290; (15, 16αB1) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 292 to 304; (15,16αA2) hypothetical protein GLYMA_02G241100 or a mutant thereof, which is any one of the following proteins (15,16αA2-a) to (15,16αA2-e): ： (15,16αA2-a) A protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 306; (15,16αA2-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO: 306; (15,16αA2-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 305 have been deleted, substituted, inserted or added; (15,16αA2-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 305; or (15,16αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 305; or (15, 16αB2) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 307 to 318; (17α)(17αA1) guanosine nucleotide diphosphate dissociation inhibitor 2 or a mutant thereof, which is any one of the following (17αA1-a) to (17αA1-e): Protein: (17αA1-a) A protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 320; (17αA1-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO: 320; (17αA1-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 319 have been deleted, substituted, inserted or added; (17αA1-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 319; or (17αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 319; (17αB1) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 321 to 325; (17αA2)rab GDP dissociation inhibitor alpha-like or a variant thereof, Any of the following proteins (17αA2-a) to (17αA2-e): (17αA2-a) A protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 327; (17αA2-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO: 327; (17αA2-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 326 have been deleted, substituted, inserted or added; (17αA2-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 326; or (17αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a base sequence complementary to the base sequence shown in SEQ ID NO: 326; or (17αB2) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 328 to 332; (18α) (18αA) hypothetical protein GLYMA_10G028300 or a variant thereof, which is any one of the following proteins (18αA-a) to (18αA-e): (18αA-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 334; (18αA-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 334; (18αA-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 333 have been deleted, substituted, inserted or added; (18αA-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 333; or (18αA-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 333; or (18αB) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 335 to 339; (19α) (19αA) sucrose binding protein homolog S-64 or a mutant thereof, which is any one of the following proteins (19αA-a) to (19αA-e): (19αA-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 341; (19αA-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 341; (19αA-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 340 have been deleted, substituted, inserted or added; (19αA-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 340; or (19αA-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 340; or (19αB) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 342 to 346; (25α) (25αA1) succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial or a mutant thereof, which is any one of the following (25αA1-a) to (25αA1-e): One of the proteins: (25αA1-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 348; (25αA1-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 348; (25αA1-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 347 have been deleted, substituted, inserted or added; (25αA1-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 347; or (25αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence represented by SEQ ID NO: 347; (25αB1) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 349 to 356; (25αA2) succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial or is a mutant thereof, and is any one of the following proteins (25αA2-a) to (25αA2-e): (25αA2-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 358; (25αA2-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 358; (25αA2-c) One or several nucleotides are deleted or substituted in SEQ ID NO: 357 a protein comprising an amino acid sequence encoded by the inserted or added nucleotide sequence; (25αA2-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 357; or (25αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 357; or (25αB2) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 359 to 365; (26α) (26αA1) phosphoglycerate kinase, cytosolic or a mutant thereof, which is any one of the following proteins (26αA1-a) to (26αA1-e): (26αA1-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 367; (26αA1-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO: 367; (26αA1-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 366 have been deleted, substituted, inserted or added; (26αA1-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 366; or (26αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence represented by SEQ ID NO: 366; (26αB1) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 368 to 382; (26αA2) phosphoglycerate kinase, cytosolic or a mutant thereof, which is any one of the following proteins (26αA2-a) to (26αA2-e): (26αA2-a) A protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 384; (26αA2-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 384; (26αA2-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 383 have been deleted, substituted, inserted or added; (26αA2-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 383; or (26αA2-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence represented by SEQ ID NO: 383; (26αB2) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 385 to 392; (26αA3) phosphoglycerate kinase, cytosolic or a mutant thereof, which is any one of the following proteins (26αA3-a) to (26αA3-e): (26αA3-a) A protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 394; (26αA3-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 394; (26αA3-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 393 have been deleted, substituted, inserted or added; (26αA3-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 393; or (26αA3-e) An amino acid encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO:

393. a protein comprising the amino acid sequence; or (26αB3) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 395 to 409; (27α)(27αA1) alcohol dehydrogenase 1 or a variant thereof, Any of proteins 27αA1-a) to (27αA1-e): (27αA1-a) A protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 411; (27αA1-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO: 411; (27αA1-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 410 have been deleted, substituted, inserted or added; (27αA1-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 410; or (27αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 410; (27αB1) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 412 to 416; (27αA2) alcohol dehydrogenase 1 or a variant thereof, Any of proteins 2-a) to (27αA2-e): (27αA2-a) A protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 418; (27αA2-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 418; (27αA2-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 417 have been deleted, substituted, inserted or added; (27αA2-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 417; or (27αA2-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 417; (27αB2) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 419 and 420; (27αA3) alcohol dehydrogenase 1-like or a mutant thereof, which is any one of the following proteins (27αA3-a) to (27αA3-e): (27αA3-a) A protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 422; (27αA3-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 422; (27αA3-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 421 have been deleted, substituted, inserted or added; (27αA3-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 421; or (27αA3-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a base sequence complementary to the base sequence shown in SEQ ID NO: 421; or (27αB3) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 423 to 427; (28α) (28αA) 35 kDa seed maturation protein isoform X1 or its mutant Any of the following proteins (28αA-a) to (28αA-e): (28αA-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 429; (28αA-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 429; (28αA-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 428 have been deleted, substituted, inserted or added; (28αA-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 428; or (28αA-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 428; or (28αB) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 430 to 433; (29-33α) (29-33αA1) glyceraldehyde-3-phosphate dehydrogenase isoform X1 or a mutant thereof, Any of the proteins of 1-e): (29-33αA1-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 435; (29-33αA1-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 435; (29-33αA1-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 434 have been deleted, substituted, inserted or added; (29-33αA1-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 434; or (29-33αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence represented by SEQ ID NO: 434; (29-33αB1) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 436 to 439; (29-33αA2) glyceraldehyde-3-phosphate dehydrogenase, cytosolic or its A mutant of any of the following proteins (29-33αA2-a) to (29-33αA2-e): (29-33αA2-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 441; (29-33αA2-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO: 441; (29-33αA2-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 440 have been deleted, substituted, inserted or added; (29-33αA2-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 440; or (29-33αA2-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence represented by SEQ ID NO: 440; (29-33αB2) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 442 to 445; (29-33αA3) uncharacterized protein LOC100782924 or a mutant thereof, which is any of the following proteins (29-33αA3-a) to (29-33αA3-e): (29-33αA3-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 447; (29-33αA3-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 447; (29-33αA3-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 446 have been deleted, substituted, inserted or added; (29-33αA3-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 446; or (29-33αA3-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 446; or (29-33αB3) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 448 to 451; (34α) (34αA1) A bifunctional UDP-glucose 4-epimerase and UDP-xylose 4-epimerase 1-like or a variant thereof, which is any one of the following proteins (34αA1-a) to (34αA1-e): (34αA1-a) A protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 453; (34αA1-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 453; (34αA1-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 452 have been deleted, substituted, inserted or added; (34αA1-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 452; or (34αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence represented by SEQ ID NO: 452; (34αB1) a protein comprising the amino acid sequence represented by SEQ ID NO: 454; (34αA2) uncharacterized protein LOC100803483 or a mutant thereof, which is any of the following proteins (34αA2-a) to (34αA2-e): (34αA2-a) A protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 456; (34αA2-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 456; (34αA2-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 455 have been deleted, substituted, inserted or added; (34αA2-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 455; or (34αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 455; or (34αB2) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 457 and 458; (35α) (35αA1) elongation factor 1-alpha or a variant thereof, comprising: Any of proteins (35αA1-a) to (35αA1-e): (35αA1-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 460; (35αA1-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 460; (35αA1-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 459 have been deleted, substituted, inserted or added; (35αA1-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 459; or (35αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence represented by SEQ ID NO: 459; (35αB1) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 461 to 466; (35αA2) elongation factor 1-alpha or a variant thereof, Any of proteins A2-a) to (35αA2-e): (35αA2-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 468; (35αA2-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 468; (35αA2-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 467 have been deleted, substituted, inserted or added; (35αA2-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 467; or (35αA2-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 467; (35αB2) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 469 to 471; (35αA3) elongation factor-1A isoform X1 or a mutant thereof, comprising the following (3 Any of the proteins 5αA3-a) to (35αA3-e): (35αA3-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 473; (35αA3-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 473; (35αA3-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 472 have been deleted, substituted, inserted or added; (35αA3-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 472; or (35αA3-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 472; or (35αB3) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 474 to 477; (36,37α) (36,37αA1) seed maturation protein PM34 or a mutant thereof, which is any one of the following proteins (36,37αA1-a) to (36,37αA1-e): (36,37αA1-a) A protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 479; (36,37αA1-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 479; (36,37αA1-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 478 have been deleted, substituted, inserted or added; (36,37αA1-d) 70% or more identity to the base sequence shown in SEQ ID NO: 478 A protein comprising an amino acid sequence encoded by the nucleotide sequence of (36,37αA1-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 478; (36, 37αB1) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 480 to 483; (36,37αA2) uncharacterized protein LOC100809384 or a mutant thereof, which is any one of the following proteins (36,37αA2-a) to (36,37αA2-e): (36,37αA2-a) A protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 485; (36,37αA2-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 485; (36,37αA2-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 484 have been deleted, substituted, inserted or added; (36,37αA2-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 484; or (36,37αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 484; (36,37αB2) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 486 and 487; (36,37αA3) hypothetical protein GLYMA_10G155300 or a variant thereof, which is any one of the following proteins (36,37αA3-a) to (36,37αA3-e): (36,37αA3-a) A protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 489; (36,37αA3-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 489; (36,37αA3-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 488 have been deleted, substituted, inserted or added; (36,37αA3-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 488; or (36,37αA3-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 488; or (36, 37αB3) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 490 to 493; (38α) (38αA) seed biotin-containing protein SBP65-like isoform X1 or a mutant thereof, which is any one of the following proteins (38αA-a) to (38αA-e): (38αA-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 495; (38αA-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 495; (38αA-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 494 have been deleted, substituted, inserted or added; (38αA-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 494; or (38αA-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 494; or (38αB) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 496 to 513; (39α) (39αA) ATP synthase subunit O, mitochondrial-like or a mutant thereof, which is any one of the following proteins (39αA-a) to (39αA-e): (39αA-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO:515; (39αA-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO:515; (39αA-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO:514 have been deleted, substituted, inserted or added; (39αA-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 514; or (39αA-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 514; or (39αB) a protein comprising the amino acid sequence represented by SEQ ID NO:516; (40,56α)(40,56αA)P24 oleosin isoform A or a mutant thereof, Any of the following proteins (40,56αA-a) to (40,56αA-e): (40,56αA-a) A protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO:518; (40,56αA-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO:518; (40,56αA-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO:517 have been deleted, substituted, inserted or added; (40,56αA-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO:517; or (40,56αA-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO:517; or (40,56αB) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 519 and 520; (41α) (41αA) uncharacterized protein At3g49720-like isoform X2 or its A mutant of any of the following proteins (41αA-a) to (41αA-e): (41αA-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO:522; (41αA-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO:522; (41αA-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 521 have been deleted, substituted, inserted or added; (41αA-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 521; or (41αA-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 521; or (41αB) a protein comprising the amino acid sequence represented by SEQ ID NO:523; (42,43α) (42,43αA1) superoxide dismutase [Mn], mitochondrial or a mutant thereof, which is any one of the following (42,43αA1-a) to (42,43αA1-e): Any protein: (42,43αA1-a) A protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO:525; (42,43αA1-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO:525; (42,43αA1-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO:524 have been deleted, substituted, inserted or added; (42,43αA1-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 524; or (42,43αA1-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence represented by SEQ ID NO:524; (42, 43αB1) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 526 to 531; (42,43αA2) hypothetical protein GLYMA_04G221300 or a variant thereof, which is any one of the following proteins (42,43αA2-a) to (42,43αA2-e): (42,43αA2-a) A protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO:533; (42,43αA2-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO:533; (42,43αA2-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO:532 have been deleted, substituted, inserted or added; (42,43αA2-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 532; or (42,43αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO:532; or (42, 43αB2) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 534 to 539; (45α) (45αA1) uncharacterized protein LOC100499771 or a mutant thereof, which is any one of the following proteins (45αA1-a) to (45αA1-e): (45αA1-a) A protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO:541; (45αA1-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence shown in SEQ ID NO:541; (45αA1-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 540 have been deleted, substituted, inserted or added; (45αA1-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 540; or (45αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence represented by SEQ ID NO:540; (45αB1) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 542 to 545; (45αA2) hypothetical protein GLYMA_09G192800 or a variant thereof, which is any one of the following proteins (45αA2-a) to (45αA2-e): (45αA2-a) One or several amino acids are deleted, substituted or inserted in SEQ ID NO: 547 a protein comprising the inserted or added amino acid sequence; (45αA2-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO:547; (45αA2-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 546 have been deleted, substituted, inserted or added; (45αA2-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 546; or (45αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 546; or (45αB2) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 548 to 551; (46α) (46αA) seed maturation protein PM22 or a mutant thereof, which is any one of the following proteins (46αA-a) to (46αA-e): (46αA-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 553; (46αA-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO:553; (46αA-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 552 are deleted, substituted, inserted or added; (46αA-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 552; or (46αA-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 552; or (46αB) a protein comprising the amino acid sequence represented by SEQ ID NO:554; (47α) (47αA1) eukaryotic translation initiation factor 5A-2 or its variants A variant of any of the following proteins (47αA1-a) to (47αA1-e): (47αA1-a) A protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 556; (47αA1-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO:556; (47αA1-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 555 have been deleted, substituted, inserted or added; (47αA1-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 555; or (47αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence represented by SEQ ID NO:555; (47αB1) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 557 and 558; (47αA2) uncharacterized protein LOC100305510 or a mutant thereof, which is any of the following proteins (47αA2-a) to (47αA2-e): (47αA2-a) A protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO:560; (47αA2-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO:560; (47αA2-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 559 have been deleted, substituted, inserted or added; (47αA2-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 559; or (47αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 559; or (47αB2) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 561 to 563; (48α) (48αA) uncharacterized protein LOC100306570 or a mutant thereof, which is any one of the following proteins (48αA-a) to (48αA-e): (48αA-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 565; (48αA-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO:565; (48αA-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 564 have been deleted, substituted, inserted or added; (48αA-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 564; or (48αA-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 564; or (48αB) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 566 to 570; (50α) (50αA) uncharacterized protein LOC100813859 or a mutant thereof, which is any one of the following proteins (50αA-a) to (50αA-e): (50αA-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO:572; (50αA-b) a protein comprising an amino acid sequence having 70% or more identity to the amino acid sequence set forth in SEQ ID NO:572; (50αA-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO:571 have been deleted, substituted, inserted or added; (50αA-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 571; or (50αA-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 571; or (50αB) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 573 to 576; (51α)(51αA)14-3-3-like protein or a mutant thereof, Any of proteins A-a) to (51αA-e): (51αA-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO:578; (51αA-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO:578; (51αA-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 577 have been deleted, substituted, inserted or added; (51αA-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 577; or (51αA-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 577; or (51αB) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 579 to 582; (52α) (52αA) probable fructokinase-4 or a variant thereof, Any of proteins 2αA-a) to (52αA-e): (52αA-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 584; (52αA-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO:584; (52αA-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 583 have been deleted, substituted, inserted or added; (52αA-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 583; or (52αA-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 583; or (52αB) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 585 to 588; (53α)(53αA1) triosephosphate isomerase isoform X1 or a mutant thereof, which is any one of the following proteins (53αA1-a) to (53αA1-e): (53αA1-a) A protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO:590; (53αA1-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO:590; (53αA1-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO:589 have been deleted, substituted, inserted or added; (53αA1-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 589; or (53αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO:589; (53αB1) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 591 to 597; (53αA2) triosephosphate isomerase, cytosolic or a mutant thereof, which is any one of the following proteins (53αA2-a) to (53αA2-e): (53αA2-a) A protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO:599; (53αA2-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO:599; (53αA2-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO:598 have been deleted, substituted, inserted or added; (53αA2-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 598; or (53αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 598; or (53αB2) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 600 to 604; (54α) (54αA1) superoxide dismutase [Fe], chloroplastic isoform X1 or The mutant is any one of the following proteins (54αA1-a) to (54αA1-e): (54αA1-a) A protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 606; (54αA1-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 606; (53αA1-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 605 have been deleted, substituted, inserted or added; (54αA1-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 605; or (54αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence represented by SEQ ID NO: 605; (54αB1) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 607 to 611; (54αA2) iron-superoxide dismutase or a mutant thereof, Any of proteins 2-a) to (54αA2-e): (54αA2-a) A protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 613; (54αA2-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 613; (54αA2-c) A protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO:612 have been deleted, substituted, inserted or added; (54αA2-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 612; or (54αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid consisting of a base sequence complementary to the base sequence shown in SEQ ID NO: 612; or (54αB2) A protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 614 to 616; or (55α) (55αA1) 51 kDa seed maturation protein precursor or a mutant thereof, comprising any one of the following proteins (55αA1-a) to (55αA1-e): (55αA1-a) a protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 618; (55αA1-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 618; (55αA1-c) a protein comprising an amino acid sequence encoded by a base sequence in which one or several nucleotides in SEQ ID NO: 617 have been deleted, substituted, inserted or added; (55αA1-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 617; or (55αA1-e) a protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 617; (55αB1) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 619 to 635; (55αA2) Lea protein precursor or a mutant thereof, comprising the following (55αA2- Any of proteins a) to (55αA2-e): (55αA2-a) A protein comprising an amino acid sequence in which one or several amino acids are deleted, substituted, inserted or added in SEQ ID NO: 637; (55αA2-b) a protein comprising an amino acid sequence having an identity of 70% or more to the amino acid sequence set forth in SEQ ID NO: 637; (55αA2-c) One or several nucleotides are deleted or substituted in SEQ ID NO: 636 a protein comprising an amino acid sequence encoded by the inserted or added nucleotide sequence; (55αA2-d) a protein comprising an amino acid sequence encoded by a base sequence having an identity of 70% or more to the base sequence shown in SEQ ID NO: 636; or (55αA2-e) A protein comprising an amino acid sequence encoded by a nucleic acid that hybridizes under stringent conditions with a nucleic acid having a base sequence complementary to the base sequence shown in SEQ ID NO: 636; or (55αB2) a protein comprising at least one amino acid sequence selected from the group consisting of SEQ ID NOs: 638 to 649; The diagnostic kit as described above, comprising as an antigen.

3. A diagnostic composition for soybean allergy, comprising at least one of the proteins identified as (1) to (8) in claim 1, or at least one of the proteins identified as (1α) to (55α) in claim 2 as an antigen.

4. 1. A method for providing an indicator for diagnosing soy allergy in a subject, comprising the steps of: (i) contacting a sample obtained from a subject with an antigen, wherein the sample is a solution containing Ig antibodies; (ii) detecting binding of an IgE antibody to the antigen in a sample obtained from a subject; (iii) detecting binding between the subject's IgE antibodies and the antigen provides an indication that the subject is allergic to soy; wherein the antigen is at least one of the proteins specified in claim 1 as (1) to (8), or at least one of the proteins specified in claim 2 as (1α) to (55α).

5. A pharmaceutical composition comprising at least one of the proteins identified as (1) to (8) in claim 1, or at least one of the proteins identified as (1α) to (55α) in claim 2.

6. The pharmaceutical composition according to claim 5 for treating soybean allergy.

7. A soybean or soybean processed product characterized in that an antigen has been removed or reduced, the antigen being at least one of the proteins specified as (1) to (8) in claim 1, or at least one of the proteins specified as (1α) to (55α) in claim 2.

8. A tester for determining the presence or absence of a soybean antigen in a subject, comprising an antibody that binds to at least one of the proteins identified as (1) to (8) in claim 1, or at least one of the proteins identified as (1α) to (55α) in claim 2.

9. An antigen derived from soybean, which is at least one of the proteins specified as (1) to (8) in claim 1 or at least one of the proteins specified as (1α) to (55α) in claim 2, and which causes allergy to soybean.

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