Liquid detergent composition for textile product for norovirus inactivation

Abstract

To provide a liquid detergent composition for a textile product for norovirus inactivation, which is effective in inactivating norovirus, and a method for inactivating norovirus using the same.SOLUTION: A liquid detergent composition for a textile product for norovirus inactivation contains (A) a surfactant and (B) an acrylic acid-maleic acid copolymer or a salt thereof.SELECTED DRAWING: None

Description

[Technical field]

[0001] The present invention relates to a liquid detergent composition for textile products for inactivating norovirus. [Background technology]

[0002] Norovirus is a non-enveloped (membrane-like structure) RNA virus classified in the Caliciviridae family and Norovirus genus. It is known to have strong resistance to acid (gastric acid) and to be infectious in small amounts (approximately 10 to 100 viruses). At present, there is no vaccine or treatment for norovirus, so it is important to prevent infection by removing or inactivating the virus by cleaning and disinfecting cooking utensils, clothing, hands, etc., which may become contaminated by the virus.

[0003] Norovirus has strong physicochemical resistance, so disinfectants containing ethanol or cationic surfactants, which are effective against many bacteria, may not be sufficiently effective against norovirus when used in a general manner. For this reason, chlorine-based disinfectants (sodium hypochlorite, etc.), iodine agents (povidone-iodine, etc.), aldehyde agents (glutaral, etc.), peracetic acid preparations, etc. are used to inactivate norovirus. However, these general disinfectants are highly irritating to mucous membranes and skin, and their use is limited due to safety concerns. In addition, chlorine-based disinfectants have the effect of bleaching the color and pattern of textiles, which limits their use on textile products such as clothing.

[0004] Acrylic acid-maleic acid copolymer is one of the water-soluble polymers of the acrylic acid series. It has excellent performance in terms of adsorption to inorganic salts by the carboxyl group and dispersion power by ionization, and is therefore used in applications such as detergent builders, sequestering agents, and scale inhibitors (calcium carbonate, phosphates, etc.). On the other hand, Patent Document 1 reports that a disinfectant preparation containing an anionic surfactant and an acidic polymer containing maleic acid as a structural unit has both antiviral and antibacterial activities. However, it has not been known that an acrylic acid-maleic acid copolymer or a combination of said polymer with a surfactant has a norovirus inactivating effect. [Prior art documents] [Patent documents]

[0005] [Patent Document 1] Special Publication No. 2011-511764 Summary of the Invention [Problem to be solved by the invention]

[0006] The present invention relates to providing a liquid detergent composition for textile products for inactivating norovirus, which is effective in inactivating norovirus, and a method for inactivating norovirus using the same. [Means for solving the problem]

[0007] The present inventors have found that a combination of a surfactant and an acrylic acid-maleic acid copolymer exhibits an excellent inactivation effect against human norovirus.

[0008] That is, the present invention relates to the following 1) to 3). 1) A liquid detergent composition for inactivating norovirus for textile products, comprising (A) a surfactant and (B) an acrylic acid-maleic acid copolymer or a salt thereof. 2) A method for cleaning textile products, which comprises contacting the textile product with the above-described liquid detergent composition for inactivating norovirus. 3) A method for inactivating norovirus, which comprises contacting a textile product that is suspected to be contaminated with norovirus with the above-described liquid detergent composition for inactivating norovirus. Effect of the Invention

[0009] According to the present invention, norovirus attached to textile products such as clothing can be inactivated, and the spread of norovirus infection can be prevented or reduced. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0010] The liquid detergent composition for textile products for inactivating norovirus of the present invention contains (A) a surfactant (hereinafter also referred to as component (A)) and (B) an acrylic acid-maleic acid copolymer or a salt thereof (hereinafter also referred to as component (B)).

[0011] The component (A) may be at least one surfactant selected from an anionic surfactant, a cationic surfactant, an amphoteric surfactant, and a nonionic surfactant, and is preferably at least one surfactant selected from an anionic surfactant and a nonionic surfactant.

[0012] The anionic surfactant is preferably a sulfuric acid, sulfonic acid or carboxylic acid surfactant, and examples thereof include alkyl sulfates, alkylbenzene sulfonates, internal olefin sulfonates having from 10 to 18 carbon atoms, polyoxyalkylene alkyl ether sulfates, polyoxyalkylene alkenyl ether sulfates, polyoxyalkylene alkylaryl ether sulfates, fatty acid salts, pyrophosphates, lauryl phosphoric acid, polycarboxylic acid type polymers, polyoxyethylene alkylene alkyl acetates, aromatic sulfonate formalin condensates, polyoxyethylene distyrenated ether sulfates, alkyl diphenyl ether disulfonates, dialkyl sulfosuccinates, and alkyl naphthalene sulfonates. Examples of the salt include alkali metal salts, alkaline earth metal salts, ammonium salts, organic ammonium salts, and basic amino acid salts. The number of carbon atoms in the alkyl portion, etc. (excluding internal olefin sulfonates having 10 to 18 carbon atoms) is preferably 8 or more, more preferably 10 or more, from the viewpoint of surfactant action, and is preferably 24 or less, more preferably 22 or less, and even more preferably 20 or less.

[0013] As the cationic surfactant, quaternary ammonium type surfactants are preferred, and examples thereof include alkylbenzyldimethylammonium salts, dialkyldimethylammonium salts, alkyltrimethylammonium salts, and triethanolamine diester quaternary salts. From the viewpoint of surfactant action, the number of carbon atoms in the alkyl portion is preferably 8 or more, more preferably 10 or more, and is preferably 22 or less, more preferably 20 or less, and further preferably 18 or less.

[0014] As the amphoteric surfactant, betaine type and amine oxide type are preferable, and examples thereof include carbobetaines such as alkylcarbobetaine and alkyl(amidopropyl)carbobetaine, sulfobetaines such as alkylsulfobetaine, alkyl(amidopropyl)sulfobetaine and alkylhydroxysulfobetaine, and alkyldimethylamine oxide. From the viewpoint of surfactant action, the number of carbon atoms in the alkyl portion is preferably 6 or more, more preferably 8 or more, and even more preferably 10 or more, and is preferably 22 or less, more preferably 20 or less, and even more preferably 18 or less.

[0015] Examples of nonionic surfactants include those having a polyoxyethylene chain, such as polyoxyethylene alkyl ethers, polyoxyethylene alkenyl ethers, polyoxyethylene polyoxypropylene glycols, polyoxyethylene polyoxypropylene alkyl ethers, polyoxyethylene polyoxypropylene alkenyl ethers, polyoxyethylene fatty acid esters, polyoxyethylene resin acid esters, polyoxyethylene glycerin fatty acid esters, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene sorbitol fatty acid esters, polyoxyethylene castor oil, polyoxyethylene hydrogenated castor oil, polyoxyethylene alkylamines, and polyoxyethylene alkyl ethers (C12-14 secondary alcohols); polyglycerin fatty acid esters, glycerin fatty acid esters, ethylene glycol fatty acid esters, propylene glycol fatty acid esters, butylene glycol fatty acid esters, sorbitan fatty acid esters, sucrose fatty acid esters, and alkyl glucosides.

[0016] The number of moles of ethylene oxide added in the polyoxyethylene chain is expressed as an average value and is preferably 2 or more, more preferably 3 or more, and even more preferably 4 or more, and is preferably 60 or less, more preferably 50 or less, more preferably 40 or less, even more preferably 30 or less, even more preferably 25 or less, and even more preferably 20 or less. The number of carbon atoms in the fatty acid moiety, alkyl moiety, and alkenyl moiety of the nonionic surfactant is preferably 6 or more, more preferably 8 or more, and even more preferably 10 or more, and is preferably 24 or less, more preferably 22 or less, and even more preferably 20 or less. The term "having a polyoxyethylene chain" refers to a structure in which one mole or more of ethylene oxide is added to the molecule of a nonionic surfactant.

[0017] Component (A) can be a commercially available product. It is also possible to produce the same substance as the commercially available product or a composition containing the same by chemical synthesis based on previously reported methods.

[0018] The content of the component (A) in the norovirus-inactivating liquid detergent composition for textile products of the present invention is preferably 1 to 70 mass %, more preferably 10 to 60 mass %.

[0019] The component (B) is a copolymer containing a structural unit derived from acrylic acid and a structural unit derived from maleic acid. The polymerization mode of the acrylic acid-maleic acid copolymer is not particularly limited and may be any of random copolymerization, block copolymerization, etc., but is preferably random copolymerization. In the acrylic acid-maleic acid copolymer of the present invention, acrylic acid and maleic acid are essential components as monomers. Maleic acid and acrylic acid may be partially or completely neutralized. Maleic acid may be maleic anhydride. In addition, in the present invention, monomer components may contain monomers other than acrylic acid (salt) and maleic acid (salt) within the range that does not impair the effects of the present invention. Such a monomer may be any monomer capable of copolymerizing with acrylic acid (salt) and maleic acid (salt), and examples thereof include unsaturated monocarboxylic acid monomers such as methacrylic acid and crotonic acid; neutralized products obtained by partially or completely neutralizing the above unsaturated monocarboxylic acid monomers with monovalent metals, divalent metals, ammonia, organic amines, etc.; unsaturated dicarboxylic acid monomers such as fumaric acid, itaconic acid, citraconic acid; neutralized products obtained by partially or completely neutralizing the above unsaturated dicarboxylic acid monomers with monovalent metals, divalent metals, ammonia, organic amines, etc.; amide monomers such as (meth)acrylamide and t-butyl(meth)acrylamide; (meth) Hydrophobic monomers such as acrylic acid esters, styrene, 2-methylstyrene, and vinyl acetate; unsaturated sulfonic acid monomers such as vinyl sulfonic acid, allyl sulfonic acid, methallyl sulfonic acid, styrene sulfonic acid, 2-acrylamido-2-methylpropanesulfonic acid, 3-allyloxy-2-hydroxypropanesulfonic acid, sulfoethyl (meth)acrylate, sulfopropyl (meth)acrylate, 2-hydroxysulfopropyl (meth)acrylate, and sulfoethylmaleimide; neutralized products obtained by partially or completely neutralizing the above unsaturated sulfonic acid monomers with monovalent metals, divalent metals, ammonia, organic amines, and the like;3-Methyl-2-buten-1-ol (prenol), 3-methyl-3-buten-1-ol (isoprenol), 2-methyl-3-buten-2-ol (isoprene alcohol), 2-hydroxyethyl (meth)acrylate, polyethylene glycol mono(meth)acrylate, polypropylene glycol mono(meth)acrylate, polyethylene glycol monoisoprenol ether, polypropylene glycol monoisoprenol ether, polyethylene glycol monoallyl ether, polypropylene glycol monoallyl ether, glycerol monoallyl ether Examples of the monomers include, but are not limited to, hydroxyl-containing unsaturated monomers such as acrylate, α-hydroxyacrylic acid, N-methylol (meth)acrylamide, glycerol mono(meth)acrylate, and vinyl alcohol; cationic monomers such as dimethylaminoethyl (meth)acrylate and dimethylaminopropyl (meth)acrylamide; nitrile monomers such as (meth)acrylonitrile; and phosphorus-containing monomers such as (meth)acrylamidomethanephosphonic acid, (meth)acrylamidomethanephosphonic acid methyl ester, and 2-(meth)acrylamido-2-methylpropanephosphonic acid. These monomers may be used alone or in combination of two or more. Examples of the salt of the acrylic acid-maleic acid copolymer include alkali metal salts, alkaline earth metal salts, ammonium salts, alkyl or alkenyl ammonium salts having 1 to 22 carbon atoms, alkyl or alkenyl-substituted pyridinium salts having 1 to 22 carbon atoms, alkanol ammonium salts having 1 to 22 carbon atoms, basic amino acid salts, etc. Alkali metal salts are preferred, and sodium salts are more preferred.

[0020] The weight average molecular weight of the component (B) is preferably 1,000 to 100,000 from the viewpoint of the norovirus inactivation effect. The weight average molecular weight can be measured, for example, by gel permeation chromatography (GPC).

[0021] The molar ratio of the constituent units derived from acrylic acid and the constituent units derived from maleic acid that constitute the component (B) (acrylic acid:maleic acid) is preferably 0.20:0.80 to 0.80:0.20 from the standpoint of norovirus inactivation effect.

[0022] Component (B) can be a commercially available product. It is also possible to produce the same substance as the commercially available product or a composition containing the same by chemical synthesis based on previously reported methods.

[0023] The content of component (B) in the norovirus-inactivating liquid detergent composition for textile products of the present invention is preferably 0.001 to 99 mass %, more preferably 0.1 to 10 mass %.

[0024] The liquid detergent composition for textile products for inactivating norovirus of the present invention can contain, in addition to the above-mentioned components (A) and (B), optional components such as foam boosters, enzymes, pH adjusters, stabilizers, fluorescent dyes, fragrances, colorants, preservatives, solvents, etc., within the range that does not impair the effects of the present invention.

[0025] The liquid detergent composition for textile products for inactivating norovirus of the present invention may contain water. The water may be the remainder excluding the component (A), the component (B) and any optional components. Examples of water include tap water, well water, ion-exchanged water, sterilized water, distilled water, etc.

[0026] The liquid detergent composition for textile products for inactivating norovirus of the present invention can be produced by any suitable method. For example, it can be produced by mixing component (A), component (B), and other components as necessary. The order of mixing the components is not particularly limited and they can be mixed in any order.

[0027] As shown in the Examples described later, the combination of (A) a surfactant and (B) an acrylic acid-maleic acid copolymer or a salt thereof exhibits an excellent virus inactivation effect against human norovirus. Therefore, the combination of (A) the surfactant and (B) the acrylic acid-maleic acid copolymer or a salt thereof can be an active ingredient of a liquid detergent composition for textile products for inactivating norovirus, and can be used to produce the liquid detergent composition for textile products for inactivating norovirus. Furthermore, by bringing the norovirus-inactivating liquid detergent composition for textile products into contact with textile products that are at risk of being contaminated with norovirus, it is possible to inactivate the norovirus adhering to the textile products.

[0028] Noroviruses include HNV (human norovirus), FCV (feline calicivirus), MNV (murine norovirus), and the like. Noroviruses are divided into seven genogroups (GI to GVII) based on the homology of their genomic base sequences, and the HNVs that infect humans are nine GI species (GI.1, GI.2, GI.3, GI.4, GI.5, GI.6, GI.7, GI.8, GI.9), 19 GII species (GII.1, GII.2, GII.3, GII.4, GII.5, GII.6, GII.7, GII.8, GII.9, GII.10, GII.12, GII.13, GII.14, GII.15, GII.16, GII.17, GII.20, GII.21, GII.22), and one GIV species (GIV.1); however, the HNV of the present invention may be of any of these genotypes.

[0029] In the present invention, inactivation of norovirus means the action of reducing or eliminating the activity of norovirus and eliminating its infectivity to host cells. The effect of inactivating human norovirus can be evaluated using a system in which human intestinal organoids are three-dimensionally cultured on an extracellular matrix, and then the intestinal epithelial cells obtained by differentiation induction are infected with HNV (see Examples).

[0030] In the present invention, examples of textile products that may be contaminated by norovirus include fabrics such as woven fabrics, knitted fabrics, and nonwoven fabrics using hydrophobic or hydrophilic fibers, and textile products obtained using these fabrics, such as undershirts, T-shirts, dress shirts, blouses, slacks, hats, handkerchiefs, towels, knitwear, socks, underwear, tights, masks, carpets, area rugs, curtains, and fabric furniture. Examples of hydrophobic fibers include protein-based fibers (milk protein casein fibers, Promix, etc.), polyamide-based fibers (nylon, etc.), polyester-based fibers (polyester, etc.), polyacrylonitrile-based fibers (acrylic, etc.), polyvinyl alcohol-based fibers (vinylon, etc.), polyvinyl chloride-based fibers (polyvinyl chloride, etc.), polyvinylidene chloride-based fibers (vinylidene, etc.), polyolefin-based fibers (polyethylene, polypropylene, etc.), polyurethane-based fibers (polyurethane, etc.), polyvinyl chloride / polyvinyl alcohol copolymer-based fibers (polycral, etc.), and the like. Examples of hydrophilic fibers include seed hair fibers (cotton, cotton, kapok, etc.), bast fibers (hemp, flax, ramie, hemp, jute, etc.), leaf vein fibers (Manila hemp, sisal, etc.), palm fibers, rush, straw, animal hair fibers (wool, mohair, cashmere, camel hair, alpaca, vicuna, angora, etc.), silk fibers (domestic silk, wild silk), feathers, and cellulosic fibers (rayon, polynosic, cupra, acetate, etc.).

[0031] The means for contacting the norovirus inactivating liquid detergent composition for textile products with a textile product is not particularly limited, and may be any of a method in which the textile product is directly contacted with the liquid detergent composition for textile products for norovirus inactivation to clean the textile product, a method in which the textile product is contacted with a cleaning liquid containing the norovirus inactivating liquid detergent composition for textile products to clean the textile product, and the like. The cleaning liquid can be prepared by diluting the norovirus inactivating liquid cleaning composition for textile products with water. For example, it can be prepared by diluting (by volume) the norovirus inactivating liquid cleaning composition for textile products of the present invention with water such as tap water used in ordinary households, preferably 100 to 5000 times, and more preferably 200 to 3000 times. Cleaning with the cleaning liquid can be carried out by contacting the cleaning liquid with the textile product at a temperature (liquid temperature) of preferably 10 to 45°C, and more preferably 20 to 35°C. After washing, the textile is preferably rinsed with water. EXAMPLES

[0032] Test Example <Preparation of test solution> A liquid detergent composition for textile products scheduled for release in Taiwan in September 2025 “Attack One Spoon Antibacterial EX Extremely Effective Anti-Infectious Poison Washing Spirit” 0.4 g of the product and 100 mL of sterilized ultrapure water were placed in a centrifuge tube, and then the tube was inverted to mix completely. The liquid detergent composition for textile products contains a surfactant and an acrylic acid-maleic acid copolymer. The human norovirus (HNV) inactivation effect of the prepared 4 g / L test solution was evaluated at Kitasato University using the method described below. Sterilized ultrapure water was used as a control.

[0033] <Human norovirus inactivation evaluation method> (i) Culture of human intestinal epithelial organoids (hIEOs) hIEOs were embedded in Matrigel (Corning) on ​​a 24-well plate and then cultured three-dimensionally at 37°C with 5% CO2. The medium used was IntestiCult Organoid Growth Medium (Human) (STEMCELL Technologies). The entire medium was replaced (500 μL / 2 days) every 2-3 days, and the cells were subcultured every 5-7 days. After 3D culture, the hIEOs were suspended in TrypLE Express (Thermo Fisher Scientific) together with the Matrigel. The hIEOs were dispersed by enzymatic treatment at 37°C for 10 minutes, and a basal medium containing Advanced DMEM / F-12 (Gibco) supplemented with 100 U / mL Penicillin-Streptomycin (Thermo Fisher Scientific), 10 mM HEPES (Thermo Fisher Scientific), and 1×GlutaMAX (Thermo Fisher Scientific) was added to stop the reaction. The cells were sedimented by centrifugation (3 minutes, 300×g, 4°C), and the above medium was then removed. The cells were suspended in differentiation medium containing basal medium supplemented with 1x B27 supplement (Thermo Fisher Scientific), 10 nM gastrin I (Sigma-Aldrich), 1 mM N-acetylcysteine ​​(Fujifilm Wako), 50 ng / mL mouse EGF recombinant protein (Thermo Fisher Scientific), 100 ng / mL mouse Noggin (Peprotech), 500 μg / mL human R-spondin-1 (R&D Systems), 500 nM A83-01 (Tocris), and 10 μM Y-27632 (Fujifilm Wako). For monolayer culture, a 96-well flat-bottom plate was prepared by coating with cell matrix type IC (Nitta Gelatin). To prepare the plate, cell matrix type IC was diluted 10-fold with 0.1 M acetic acid (Nacalai tesque). After adding 100 μL of Nitta Gelatin per well, the plate was incubated at 37°C for 90 minutes. The solution was then removed and the plate was washed three times with 200 μL of D-PBS(-) (Nacalai tesque). 4 The cells were seeded at a concentration of 100 μL / well. Differentiation was induced for a total of 6 days while changing the entire amount of the differentiation medium every 2 days, to obtain monolayered hIEOs.

[0034] (ii) Preparation of a 10% emulsion of HNV-containing feces A 10% fecal emulsion was prepared from feces of a patient infected with HNV type GII.4. One tablet of cOmplete protease inhibitor cocktail tablets (Sigma-Aldrich, 11697498001), a protease inhibitor, was suspended in 50 mL of D-PBS(-). 1 g of feces was suspended in 10 mL of cOmplete-containing D-PBS(-) and mixed well with a test tube mixer. After standing at 4°C for 20 minutes, the mixture was centrifuged at 2,000×g for 10 minutes at 4°C. The supernatant was collected in a new tube and stored at -80°C until used in an infection experiment.

[0035] (iii) HNV inactivation and infection of differentiated hIEOs A 10% fecal emulsion containing HNV was diluted 10-fold with sterile ultrapure water and filtered using a 1 mL syringe and a Millex HV Filter unit (Millipore, SLHVR04NL). The filtered fecal solution was collected in a PA microcentrifuge tube (Beckman Coulter). The centrifuge tube containing the filtered fecal solution was placed in a fixed-angle rotor TLA-55 (Beckman Coulter) and ultracentrifuged for 1.5 hours at a centrifugal force of 186047 × g (equivalent to 55,000 rpm) at Rmax using an Optima MAX-TL (Beckman Coulter), after which the supernatant was removed. The pellet was suspended in 50 μL of sterile ultrapure water to prepare the virus solution to be used in the test. 0.5 μL (2.8 × 10 7The test solution (49.5 μL) was mixed with 49.5 μL of the test solution in a centrifuge tube and reacted at the specified temperature and time shown in Table 1. Then, 0.95 mL of Fetal Bovine Serum (capricorn scientific) solution was added to the drug-treated fecal solution. The centrifuge tube was set in a fixed-angle rotor TLA-55, and ultracentrifuged for 1.5 hours at Rmax with an Optima MAX-TL at a centrifugal force of 186047 × g (equivalent to 55,000 rpm), and the supernatant was removed. The pellet was suspended in 100 μL of differentiation medium to prepare an infection solution for hIEOs. After removing the existing medium in the well (96-well plate), the infection solution prepared by the above method was applied to the hIEOs after differentiation induction for 6 days. Incubation was performed at 37 ° C for 1 hour. After washing three times with 300 μ L of basic medium, 250 μ L of differentiation medium was added and cultured under conditions of 37 ° C and 5% CO2 for 6 days. After 6 days of culture, 10 μL of the supernatant was collected and centrifuged at 15,000 rpm for 5 minutes to remove cell debris, and then frozen and stored at −80°C until quantification of human norovirus genome RNA.

[0036] (iv) RT-qPCR The human norovirus genome RNA in the collected supernatant was quantified using the Norovirus Detection Kit G1 / G2 (Toyobo, FIK-273) according to the protocol. The lower limit of quantification of human norovirus genome RNA was 50 copies / μL. LightCycler480II (Roche) was used for PCR amplification and data measurement. Human norovirus genome RNA [copies / μL] and the reduction rate of human norovirus genome RNA are shown in Table 1. Since two wells of cells (two technical replicates) were used per test solution in the experiment, the data for human norovirus genome RNA [copies / μL] are shown as arithmetic mean values. The reduction rate of human norovirus genome RNA was calculated using Equation 1. Since the measured values ​​in Example 1 were lower than the lower limit of quantification described above, the reduction rate of human norovirus genome RNA was calculated by applying the lower limit of quantification of 50 copies / μL to Equation 1.

[0037]

number

[0038] [Table 1]

Claims

1. A liquid detergent composition for textile products for inactivating norovirus, comprising (A) a surfactant and (B) an acrylic acid-maleic acid copolymer or a salt thereof.

2. 2. The liquid detergent composition for inactivating norovirus for textile products according to claim 1, wherein the surfactant (A) contains at least one surfactant selected from an anionic surfactant and a nonionic surfactant.

3. The liquid detergent composition for inactivating norovirus for textile products according to claim 1, wherein the norovirus is a human norovirus.

4. A method for cleaning textile products, comprising contacting the textile product with the liquid detergent composition for inactivating norovirus according to any one of claims 1 to 3.

5. A method for inactivating norovirus, comprising contacting a textile product which is suspected to be contaminated with norovirus with the liquid detergent composition for inactivating norovirus for textile products according to any one of claims 1 to 3.

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