Composition

Abstract

To provide a composition which has adiposity inhibiting effect and myoblast activating effect on adipocytes and is excellent in effect of adiposity inhibition, muscle enhancement, and the like.SOLUTION: The composition contains a digestible oligosaccharide, and at least one selected from plant products, lactobacilli and polysaccharides.SELECTED DRAWING: None

Description

[Technical field]

[0001] The present invention relates to a composition containing digestible oligosaccharides and at least one member selected from a processed plant product, a lactic acid bacterium, and a polysaccharide. [Background technology]

[0002] Conventionally, compositions containing processed plant products or polysaccharides have been known as health foods and the like (Patent Document 1). [Prior art documents] [Patent documents]

[0003] [Patent Document 1] JP 2003-267880 A Summary of the Invention [Problem to be solved by the invention]

[0004] With regard to compositions used in health foods and the like, consumer needs have become more diverse in recent years, and the demand for further improved effects, particularly in terms of health and beauty, is becoming stronger. However, conventional compositions have not been able to fully meet this demand. [Means for solving the problem]

[0005] Therefore, the present inventors have intensively studied a composition containing at least one selected from a processed plant product, lactic acid bacteria, and polysaccharides, which can further enhance the effects from the viewpoints of health and beauty. As a result, they have surprisingly found that a combination of digestible oligosaccharides with at least one selected from a processed plant product, lactic acid bacteria, and polysaccharides suppresses fat accumulation in adipocytes and activates myoblasts, and is useful for dieting and preventing or reducing locomotive syndrome, and have thus completed the present invention.

[0006] The present invention is based on the above findings, and provides a composition containing digestible oligosaccharides and at least one member selected from a processed plant product, a lactic acid bacterium, and a polysaccharide. Effect of the Invention

[0007] According to the present invention, a composition having excellent effects of inhibiting fat accumulation in adipocytes and activating myoblasts is provided. Also, according to the present invention, a diet composition and / or a composition for preventing locomotive syndrome can be provided, which can inhibit the accumulation of body fat such as subcutaneous fat and visceral fat by inhibiting fat accumulation in adipocytes and activating myoblasts, maintain or improve energy metabolic activity such as lipid decomposition and burning, reduce or inhibit weight gain, slim down the appearance, inhibit rebound, build muscle or prevent muscle loss, and improve the efficiency of recovery from muscle damage. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0008] The composition of the present invention will be described below based on its preferred embodiment. Hereinafter, the composition of the present invention applies to any of "oral composition", "oral agent", "edible composition", "edible agent", and "food and drink" that can be safely ingested, and also applies to any of "diet composition", "muscle building composition", "muscle reduction prevention composition", "composition for preventing and / or improving locomo syndrome", "diet agent", "muscle building agent", "muscle reduction prevention agent", "agent for preventing and / or improving locomo syndrome", "diet food", "muscle building food", "muscle reduction prevention food", and "food for preventing and / or improving locomo syndrome" that can safely obtain the effects of the present invention.

[0009] Digestive oligosaccharides The digestive oligosaccharide in the present invention is an oligosaccharide of three or more sugars that is decomposed by human digestive enzymes and absorbed in the stomach or small intestine to become energy. The number of constituent sugars of the digestive oligosaccharide that can be used in the present invention is preferably three to ten saccharides, more preferably three to eight saccharides, even more preferably three to six saccharides, and particularly preferably three to four saccharides from the viewpoint of effectiveness. Examples of digestive oligosaccharides include maltooligosaccharide, isomaltooligosaccharide, soybean oligosaccharide, etc., and maltooligosaccharide is particularly preferred from the viewpoint of availability, enhancing the effect of suppressing fat accumulation in adipocytes and activating myoblasts, and good handling during production. Specific examples of maltooligosaccharides include maltotriose, maltotetraose, maltopentaose, maltohexaose, etc., and maltotriose is particularly preferred from the viewpoint of enhancing the effect of the present invention.

[0010] The content of digestive oligosaccharides in the solid content of the composition of the present invention is preferably 0.0001% by mass or more, more preferably 0.001% by mass or more, even more preferably 0.01% by mass or more, and particularly preferably 0.05% by mass or more in terms of effect, in terms of further enhancing the effect of inhibiting fat accumulation in adipocytes and the activation of myoblasts. The composition of the present invention may be composed of only digestive oligosaccharides, processed plant products, lactic acid bacteria, and polysaccharides, but from the viewpoint of enabling high functionality and multi-functionality by combination with other components, the upper limit of the content of digestive oligosaccharides in the composition is preferably 30% by mass or less, more preferably 20% by mass or less, and even more preferably 10% by mass or less.

[0011] ·Plant processed products The processed plant product in the present invention is obtained by subjecting an orally ingestible plant body to one or more of the following processes: drying, crushing, extraction, filtration, squeezing, slurrying, fermentation, heating, etc. Examples of parts of the plant body include leaves, roots, rhizomes, flowers, stems, fruits, seeds, etc., and may include one or more parts.

[0012] When a processed plant product is obtained from a plant body, the plant body is preferably one that has been harvested or processed immediately after harvesting. If time is required before processing, the plant body is preferably stored by a storage method commonly used by those skilled in the art, such as low-temperature storage, in order to prevent deterioration of the plant body.

[0013] Specific examples of plant processed products include, but are not limited to, dried powder obtained by drying and pulverizing a plant body (hereinafter also referred to as "dried pulverized powder"); shredded plant body and its dried product; squeezed juice of a plant body and its dried powder; and extract of a plant body and its dried powder. In the present invention, it is preferable that the final product is in the form of a powder from the viewpoint of ease of processing, storage, transportation, etc. and versatility of use. In this specification, the term "powder" generally includes any of dried pulverized powder, dried powder of shredded product, dried powder of squeezed juice, dried powder of extract, and dried powder of fermented product. Powder naturally includes granular and granular forms.

[0014] A conventionally known method can be used to dry and pulverize the plant body into powder. As such a method, a method in which a drying treatment and a pulverization treatment are combined can be used for the plant body. Either the drying treatment or the pulverization treatment may be performed first, but it is preferable to perform the drying treatment first. The drying and pulverization treatment may further include one or more treatments selected from blanching treatment, cooling treatment, sterilization treatment, etc., as necessary. The pulverization treatment may be performed two or more times, and it is preferable to perform a coarse pulverization treatment followed by a fine pulverization treatment for more fine pulverization.

[0015] The drying treatment is not particularly limited, but is preferably a treatment for drying barley stems and leaves so that the moisture content is 10% by mass or less, particularly 5% by mass or less. This drying treatment can be carried out by any method known to those skilled in the art, such as hot air drying, high-pressure steam drying, electromagnetic wave drying, freeze drying, etc.

[0016] The crushing treatment is not particularly limited, and examples thereof include a treatment in which the plant body is crushed by any method commonly used by those skilled in the art using crushing equipment or tools such as a crusher, a mill, a blender, or a stone mill. The crushed plant body is sieved as necessary, and it is preferable to use, for example, a sieve that passes through a 30 to 250 mesh as the plant body powder. By making the particle size smaller than 250 mesh, the plant body powder becomes easier to handle during further processing, and by making the particle size larger than 30 mesh, it becomes easier to uniformly mix the plant body powder with other materials.

[0017] Specific examples of the drying and pulverization method include a method in which the plant body is cut, dried to a water content of 10% by mass or less, and then pulverized. Other examples include a method in which the plant body is cut, rolled, dried, and pulverized; a method in which the plant body is dried, coarsely pulverized, heated at 110° C. or higher, and then finely pulverized.

[0018] The method for shredding the plant body is not particularly limited, and any method that is commonly used by those skilled in the art when shredding a plant body, such as slicing, crushing, or chopping, may be used. As an example of shredding, the plant body may be made into a slurry. The slurry is made by putting the plant body into a mixer, juicer, blender, mass colloider, or the like to make it into a mushy porridge-like substance (a suspension of liquid and solid). When shredding the seeds is heated, the liquid may be boiled down with water, and the coarse solid content may be removed by sieving, filtration, or the like, and the liquid may be used.

[0019] The method for squeezing the plant body is not particularly limited, and examples thereof include a method of squeezing the plant body or a shredded product thereof, and a method of centrifuging or filtering the shredded product of the plant body. Specific examples of the squeezing method include a method in which the juice is squeezed by a mechanical crushing means such as a mixer or a juicer, and if necessary, a crude solid content is removed by means of sieving, filtration, or the like to obtain a squeezed juice. The squeezed juice may be concentrated or dried and powdered as necessary. When powdering, an excipient may be used as necessary.

[0020] The method for obtaining an extract of a plant body is not particularly limited, but examples thereof include a method in which an extraction solvent commonly used by those skilled in the art, such as ethanol, water, methanol, ethyl acetate, or acetone, is added to the plant body or its finely ground or dried product, and the mixture is stirred and / or heated as necessary to extract the plant body. One or more types of extraction solvents can be appropriately selected and used as necessary. When heating the mixture, the temperature is not particularly limited as long as it is between room temperature and the boiling point of the solvent. It is preferable to use an extract obtained by removing the crude solid content by means of subsequent sieving, filtration, or the like. For example, the filtrate (extract) obtained by adding water to finely ground soybeans (grinding product), boiling the mixture, and then filtering the mixture is known as soy milk. The extract may be concentrated as necessary, or may be dried and powdered. When powdering the mixture, an excipient may be used as necessary.

[0021] When the processed plant product is in a powder form, the moisture content is preferably 20% by mass or less, particularly 10% by mass or less, from the viewpoints of stability and prevention of quality deterioration, etc. For example, a moisture content of 1% by mass or more is preferred from the viewpoint of ease of production of the powdered processed plant product.

[0022] When the plant processed product is in a powder form, it is preferable that the powder passes through any one of 30 to 250 mesh sieves in terms of ease of mixing with other ingredients, ease of oral administration, etc. From the same viewpoint, it is more preferable that 90% by mass or more of the powdered plant processed product passes through a 200 mesh sieve.

[0023] The processed plant product of the present invention may be a commercially available product, for example, the one described in the Examples below.

[0024] The plant body in the processed plant product is preferably a processed product of green leaves. In the present invention, green leaves are leaves and / or stems of green plants that can be orally ingested and contain chlorophyll. The green leaves that can be used in the present invention include, for example, green leaves of Gramineae plants, Asteraceae plants, Umbelliferae plants, Mulberry plants, Houttuynia plants, Labiatae plants, Liliaceae plants, Tilia plants, Convolvulaceae plants, Theaceae plants, and the like, which are preferred because they have high fat accumulation inhibitory activity in adipocytes and myoblast activation activity, and because plant-derived nutritional components can be ingested. Examples of Gramineae plants include wheat, durum wheat, rye, triticale, barley, oats, pigeon barley, corn, rice, barnyard millet, foxtail millet, and kumazasa. Examples of Asteraceae plants include mugwort. Examples of Umbelliferae plants include angelica tree, parsley, celery, and chou-mei-sou (also called Peony Flower). Examples of Mulberry plants include mulberry. Examples of Houttuynia plants include Houttuynia cordata. Examples of Lamiaceae plants include perilla. Examples of Brassicaceae plants include komatsuna, kale, cabbage, and broccoli. Examples of Liliaceae plants include asparagus. Examples of Tiliaceae plants include mulukhiyah. Examples of Convolvulaceae plants include sweet potato. Examples of Theaceae plants include tea. Among these, barley, kale, chou-mei-sou, kumazasa, tea, angelica tree, mulberry, sweet potato, mugwort, and cabbage are particularly preferred in terms of effectiveness.

[0025] When the green leaves used in the present invention are barley leaves, it is preferable to use young barley leaves. Young barley leaves are leaves and / or stems harvested before maturity, i.e., from the start of tillering to the start of heading. Also, if the green leaves are from sweet potatoes, the leaves and / or leaves that are exposed above ground during the cultivation of sweet potatoes are It is preferable to use leaves including stems, and young sweet potato leaves are particularly preferable.

[0026] When the plant body is green leaves, the green leaves are preferably in the form of dried and pulverized powder. As described above, the dried and pulverized powder is a dry powder obtained by drying and pulverizing a plant body that has not been subjected to juice squeezing or extraction processing.

[0027] The plant processed product in the present invention includes fermented products of plants because of their high fat accumulation inhibitory activity in adipocytes and myoblast activation activity. In addition to the various plants listed as examples of processed green leaves above, other vegetables, mushrooms, grains, seeds, beans, fruits, seaweed, wild grasses, etc. can be used as plant fermented products. Specific examples of other vegetables, mushrooms, grains, seeds, beans, fruits, seaweed, and wild grasses include burdock, carrots, lotus root, cabbage, pumpkin, radish, tomato, bell pepper, shiitake mushroom, barley, millet, brown rice, pearl barley, corn, sesame, soybeans, kidney beans, pineapple, banana, apple, lemon, melon, prune, kiwi, kelp, wakame, gynostemma, horsetail, muira puama, and cat's claw, and these can be used alone or in combination of two or more. In the case where the processed plant product is a fermented product, the plant body is preferably a fermented product of 5 or more kinds, more preferably 10 or more kinds, more preferably 20 or more kinds, and particularly preferably 40 or more kinds of plant bodies selected from vegetables such as cabbage, mushrooms, grains, seeds, beans, fruits, seaweed, and wild plants. As the fermented plant product, for example, commercially available fermented products called "fermented plant extract" or "fermented wild plant extract" may be used.

[0028] When the processed plant product is a fermented product, the fermented product may be in any of the following forms: liquid, slurry, paste, and powder. Fermented products of plants include those obtained by fermenting the plant or its crushed product, juice, extract, or shredded product. When the product after fermentation is in the form of a liquid, slurry, or paste, it can be dried by any method known to those skilled in the art, such as hot air drying, high-pressure steam drying, electromagnetic wave drying, and freeze-drying. In this case, an excipient may be added, and the product may be dried and then crushed to obtain a dry powder.

[0029] Fermentation of a plant can be achieved, for example, by adding lactic acid bacteria, yeast, koji mold, natto bacteria, acetic acid bacteria, or the like to the plant or its pulverized product, shredded product, juice, or extract. These may be used in combination of one or more kinds, and when two or more kinds are used, they may be added simultaneously or different kinds may be added stepwise. It is preferable that the bacteria used for fermentation is at least one kind selected from lactic acid bacteria, yeast, and acetic acid bacteria, from the viewpoint of being able to ingest useful components such as physiologically active substances produced by these species.

[0030] The lactic acid bacteria used for fermentation refer to bacteria that produce lactic acid through metabolism, and specific examples thereof include the bacteria described as lactic acid bacteria that can be used in the present invention. Examples of yeasts used for fermentation include sake yeast, wine yeast, brewer's yeast, baker's yeast, etc. For example, yeasts belonging to the genus Saccharomyces, Schizosaccharomyces, etc. are used, and preferably Saccharomyces cerevisiae, Saccharomyces pastorianus, Schizosaccharomyces pombe, etc. In particular, Saccharomyces cerevisiae and its isolated strains are preferred in terms of producing useful substances such as amino acids and vitamins. Examples of koji molds used for fermentation include black koji mold, white koji mold, yellow koji mold, red koji mold, etc., and commercially available products can be suitably used. Specifically, examples include microorganisms belonging to the genus Aspergillus, such as Aspergillus awamori (black koji mold), Aspergillus saitoi (black koji mold), Aspergillus nakazawai (black koji mold), Aspergillus usamii (black koji mold), Aspergillus luchensis (black koji mold), Aspergillus niger (black koji mold), Aspergillus kawachii (white koji mold), and Aspergillus oryzae (yellow koji mold). Examples of acetic acid bacteria include microorganisms belonging to the genus Acetobacter, such as Acetobacter acetii, Acetobacter pasteurianus, and Acetobacter hansenii. Examples of natto bacteria include bacteria of the genus Bacillus, such as Bacillus natto, Bacillus subtilis, and Bacillus circulans.

[0031] ·Lactic acid bacteria The lactic acid bacteria of the present invention refer to bacteria that produce lactic acid by metabolism. The lactic acid bacteria that can be used in the present invention may be live or dead bacteria, and in the case of dead bacteria, the bacteria may be crushed. Lactic acid bacteria powder can be obtained, for example, by removing unnecessary parts such as a medium from a cultured lactic acid bacteria or a lactic acid bacteria culture used for fermentation, and then powdering the lactic acid bacteria cells by a known method. The type of lactic acid bacteria is not particularly limited as long as it produces lactic acid as a metabolic product, and includes those that have been orally ingested by animals such as humans, for example, those of the genus Bifidobacterium, Lactobacillus, Enterococcus, Leuconostoc, Pediococcus, Staphylococcus, Tetragenococcus, and Bacillus. Bifidobacterium genus includes Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium infantis, Bifidobacterium lactis, Bifidobacterium longum, Bifidobacterium adolescentis, and Bifidobacterium mongoliense. Lactobacillus genus includes Lactobacillus brevis, Lactobacillus gasseri, Lactobacillus acidophilus, Lactobacillus buchneri, Lactobacillus bulgaricus, Lactobacillus delburvecki, Lactobacillus casei, Lactobacillus crispatus, Lactobacillus curvatus, Lactobacillus halivaticus, Lactobacillus Examples include Lactobacillus pentosus, Lactobacillus plantarum, Lactobacillus paracasei, Lactobacillus rhamnosus, Lactobacillus salivarius, Lactobacillus sporogenes, Lactobacillus sakei, Lactobacillus fructivorans, Lactobacillus hilgardii, Lactobacillus reuteri, and Lactobacillus fermentum. Enterococcus includes Enterococcus faecalis (sometimes called Streptococcus faecalis), Enterococcus faesium (sometimes called Streptococcus faesium), Streptococcus thermophilus, and Lactococcus lactis (sometimes called Streptococcus lactis). Examples of the Leuconostoc genus include Leuconostoc mesenteroides and Leuconostoc oenos. The genus Pediococcus includes Pediococcus acidilactici and Pediococcus pentosaceus. The genus Staphylococcus includes Staphylococcus carnosus and Staphylococcus xylosus. The genus Tetragenococcus includes Tetragenococcus halophilus. Examples of the genus Bacillus include Bacillus coagulans and Bacillus mesentericus. Among these, Bacillus coagulans, Enterococcus faecalis, and Bifidobacterium bifidum are preferred because they have a high effect of inhibiting fat accumulation in adipocytes and a high effect of activating myoblasts. These may be used alone or in combination of two or more.

[0032] ·Polysaccharide The polysaccharides of the present invention are those that are not digested or are extremely difficult to digest (resistant to digestion) by human digestive enzymes. Examples of polysaccharides that can be used in the present invention include raffinose, fructooligosaccharides, galactooligosaccharides, soybean oligosaccharides, xylooligosaccharides, polydextrose, resistant dextrin, psyllium, pectin, arabinoglucan, xylan, galactomannan, glucomannan, galactan, tamarind gum, xanthan gum, etc., and polydextrose, resistant dextrin, tamarind gum, xanthan gum, etc. are preferred. Examples of water-soluble dietary fibers include psyllium, pectin, arabinoglucan, xylan, galactomannan, glucomannan, galactan, tamarind gum, and xanthan gum. Examples of water-soluble dietary fibers include polydextrose, resistant dextrin, tamarind gum, and xanthan gum. More preferred are polydextrose, resistant dextrin, xanthan gum, and tamarind gum. In particular, xanthan gum and tamarind gum are preferred because of their high effect of inhibiting fat accumulation in adipocytes and high effect of activating myoblasts.

[0033] The contents of the processed plant products, lactic acid bacteria and polysaccharides in the present invention are not particularly limited, but from the viewpoint of further enhancing the effect of inhibiting fat accumulation in adipocytes and the effect of activating myoblasts, the total content of the processed plant products, lactic acid bacteria and polysaccharides in the solid content of the composition of the present invention is preferably 10% by mass or more, more preferably 20% by mass or more, even more preferably 30% by mass or more, and particularly preferably 40% by mass or more in terms of effect, in terms of dry mass. The composition of the present invention may be composed only of digestible oligosaccharides, processed plant products, lactic acid bacteria and polysaccharides, but from the viewpoint of enabling high functionality and multi-functionality by combination with other components, the upper limit of the content of the processed plant products, lactic acid bacteria and polysaccharides in the composition is preferably 90% by mass or less, more preferably 80% by mass or less, and even more preferably 70% by mass or less. The total amount of plant processed products, lactic acid bacteria and polysaccharides in the composition referred to here is the amount of each of the plant processed products, lactic acid bacteria or polysaccharides when only one type of plant processed product, lactic acid bacteria or polysaccharide is contained, and is the total amount when two or more types of these are contained.

[0034] In terms of inhibiting lipid accumulation in adipocytes and activating myoblasts, the mass ratio of digestive oligosaccharides to the total amount of plant processed products, lactic acid bacteria and polysaccharides per 1 part by mass of digestive oligosaccharides of the composition of the present invention is preferably at least 0.01 part by mass, more preferably from 0.1 to 10,000 parts by mass, even more preferably from 1 to 1,000 parts by mass, and particularly preferably from 5 to 600 parts by mass in terms of effectiveness.

[0035] In particular, from the standpoint of further enhancing the fat accumulation prevention effect and myoblast activation activity, the mass ratio of digestible oligosaccharides to processed green leaf products is preferably 1 part by mass or more of each processed product by dry mass to 1 part by mass of digestible oligosaccharides, more preferably 25 parts by mass or more and 10,000 parts by mass or less, even more preferably 50 parts by mass or more and 1,000 parts by mass or less, and particularly preferably 100 parts by mass or more and 500 parts by mass or less from the standpoint of effectiveness. For the same reasons, the mass ratio of digestible oligosaccharides to plant fermentation product is preferably 0.01 mass parts or more of plant fermentation product per 1 mass part of digestible oligosaccharides, more preferably 0.01 mass parts or more and 100 mass parts or less, even more preferably 0.1 mass parts or more and 50 mass parts or less, and particularly preferably 1 mass part or more and 10 mass parts or less from the standpoint of effectiveness. From the same viewpoint, the mass ratio of digestive oligosaccharides to lactic acid bacteria is preferably 0.0001 parts by mass or more and 100 parts by mass or less of lactic acid bacteria per 1 part by mass of digestive oligosaccharides, more preferably 0.001 parts by mass or more and 50 parts by mass or less, and particularly preferably 0.01 parts by mass or more and 20 parts by mass or less from the viewpoint of effectiveness. From the same viewpoint, the mass ratio of digestible oligosaccharides to polysaccharides is preferably 0.1 mass parts or more of polysaccharides on a dry mass basis to 1 mass part of digestible oligosaccharides, more preferably 1 part or more and 5,000 parts or less of polysaccharides, even more preferably 10 parts or more and 1,000 parts or less of polysaccharides, and particularly preferably 20 parts or more and 500 parts or less of polysaccharides on a dry mass basis from the viewpoint of effectiveness.

[0036] The composition of the present invention may contain other components in addition to digestible oligosaccharides, processed plant products, lactic acid bacteria, and polysaccharides. Examples of the other components include vitamins, proteins, minerals, dairy products, citric acid, acidulants, colorants, glossing agents, as well as manufacturing agents such as talc and calcium stearate. Other components include various excipients, binders, lubricants, stabilizers, diluents, bulking agents, emulsifiers, colorants, flavorings, food additives, and seasonings. The content of the other components can be appropriately selected depending on the form of the composition. In this embodiment, the components other than digestible oligosaccharides, processed plant products, lactic acid bacteria, and polysaccharides contained in the composition are preferably 50% by mass or less, more preferably 30% by mass or less, and particularly preferably 10% by mass or less in terms of effect, based on the solid content.

[0037] The composition of the present invention may be in any form, such as solid, semi-solid, or fluid. For example, the solid form may be powder, fine grain, granule, tablet, capsule, rod, plate, block, solid, round, candy, gummy, wafer, biscuit, cookie, cake, chewable, etc. The semi-solid form may be paste, jelly, etc. The fluid form may be syrup, liquid, jelly, etc.

[0038] In addition, the composition of the present invention is preferably in the form of powder, fine grains or granules, and mixed with water to be taken orally, since it prevents spoilage and is suitable for long-term storage.In addition, when the composition of the present invention is in the form of a solid, as described above, it can be mixed with water to make a liquid, and the liquid can be taken orally by drinking, but it can also be taken orally as a solid, depending on the preferences of the person who takes it.In addition, it can be added to not only water, but also milk, soy milk, fruit juice drinks, whey drinks, soft drinks, yogurt, pancake mix, etc. for use.It can also be used as a supplement, health food, nutritional functional food, functional food, specified health food, and medicine.

[0039] The composition of the present invention is an oral composition, and when the composition contains a processed green leaf product as a processed plant product, it is preferable to use the composition of the present invention as an edible composition for green juice. The edible composition for green juice is a beverage containing a processed green leaf product. The edible composition for green juice includes the beverage and a solid that is dispersed or dissolved in a liquid to obtain the beverage. The edible composition for green juice includes smoothies, jellies, etc. in addition to commonly known green juice products.

[0040] The form of the composition of the present invention is not limited to the beverages given as examples of edible compositions for green juice and the solid form that is dispersed or dissolved in a liquid to obtain this beverage, and any form can be adopted.

[0041] As described in the examples below, the green leaf powder of the present invention contains digestible oligosaccharides and at least one selected from processed plant products, lactic acid bacteria, and polysaccharides, and thus has an excellent effect of inhibiting fat accumulation in adipocytes and / or activating myoblasts. Specifically, by ingesting a composition containing digestible oligosaccharides and at least one selected from processed plant products, lactic acid bacteria, and polysaccharides, it is possible to prevent lipid accumulation in adipocytes and activate myoblasts in skeletal muscles, etc. Therefore, by ingesting the composition of the present invention, it is possible to prevent body fat accumulation, reduce body fat, maintain or promote energy metabolism in muscles, promote muscle building, prevent muscle attenuation or reduction, use in dieting, and prevent or improve locomotive syndrome, etc. That is, the composition of the present invention can be excellent for preventing body fat accumulation, reducing body fat, activating myoblasts, promoting muscle cell proliferation, building muscle, preventing muscle loss, preventing muscle attenuation, promoting metabolism, preventing obesity, preventing obesity, eliminating obesity, reducing or suppressing weight gain, slimming one's appearance, preventing rebound, dieting, and preventing and / or improving locomotive syndrome, etc. Here, building muscle means increasing muscle mass, and preventing muscle loss means preventing muscle mass loss. EXAMPLES

[0042] The present invention will be described in more detail below by showing examples, but the scope of the present invention is not limited to these examples.

[0043] [Examples 1 to 20, Comparative Examples 1 to 24] The following test samples were used as described in Tables 1 and 2. Maltotriose: A commercially available powdered product was used. Trehalose: A commercially available powdered product was used. Young barley leaves: Dried and ground powder of young barley leaves manufactured by Toyo Shinyaku Co., Ltd. was used. The dried and ground powder had 90% or more by mass passing through a 200 mesh and a moisture content of 1% to 7% by mass. Kale: Dried and ground kale leaves manufactured by Toyo Shinyaku Co., Ltd. were used. More than 90% by mass of the dried and ground powder passed through a 200 mesh sieve, and the moisture content was between 1% and 7% by mass. · Chomeisou: Dried and crushed powder of Chomeisou leaves manufactured by Toyo Shinyaku Co., Ltd. The dried and crushed powder had 90% or more by mass passing through a 200 mesh and a moisture content of 1% to 7% by mass. · Kumazasa: Commercially available dried and crushed Kumazasa leaves were used. More than 90% of the dried and crushed powder passed through a 200 mesh sieve and had a moisture content of 1% to 7% by mass. Matcha: Dried and ground powder of tencha produced by Toyo Shinyaku Co., Ltd. More than 90% of the dried and ground powder passed through a 200 mesh screen and had a moisture content of 1% to 7% by mass. · Angelica keiskei: Commercially available dried and crushed powder of Angelica keiskei leaves was used. More than 90% of the dried and crushed powder passed through a 200 mesh sieve and had a moisture content of 1% to 7% by mass. Mulberry: Dried and crushed powder of mulberry leaves manufactured by Toyo Shinyaku Co., Ltd. The dried and crushed powder had 90% or more by mass passing through a 200 mesh and a moisture content of 1% to 7% by mass. · Young sweet potato leaves: We used dried and crushed powder of sweet potato leaves manufactured by Toyo Shinyaku Co., Ltd. Of the dried and crushed powder, 90% by mass or more passed through a 200 mesh and the moisture content was 1% by mass or more and 7% by mass or less. Mugwort: Dried and crushed powder of mugwort leaves manufactured by Toyo Shinyaku Co., Ltd. was used. More than 90% of the dried and crushed powder passed through a 200 mesh sieve, and the moisture content was between 1% and 7% by mass. Spore-forming lactic acid bacteria: A commercially available dried powder of spores of the scientific name Bacillus coagulans was used. Lactic acid bacteria (killed): Commercially available dried powder of killed bacteria of the scientific name Enterococcus faecalis was used. Lactic acid bacteria (mixture): A mixture of commercially available dry powders of live bacteria with the scientific names Enterococcus faecium and Bifidobacterium bifidum was used. Polydextrose: Commercially available polydextrose (powder) with a dietary fiber content of 90% or more by mass was used. - Resistant dextrin: Commercially available resistant dextrin (powder) with a dietary fiber content of 85-95% by mass was used. -Xanthan gum: Commercially available xanthan gum (powder) derived from Xanthomonas Campestris was used. Tamarind gum: Commercially available tamarind gum (dried powder) derived from tamarind seeds was used. Fermented cabbage extract: Toyo Shinyaku Co., Ltd.'s product name "Fermented cabbage extract" (dried and crushed powder made by fermenting raw cabbage with lactic acid bacteria and then drying and crushing it) was used. -Multi-plant fermented extract: A commercially available multi-plant fermented extract was used (raw materials including more than 80 types of vegetables, fruits, grains and wild plants such as burdock, carrots, lotus root, cabbage, pumpkin, radish, tomato, bell pepper, shiitake mushroom, barley, millet, brown rice, pearl barley, corn, sesame, soybeans, kidney beans, pineapple, banana, apple, lemon, melon, prune, kiwi, kelp, wakame, gynostemma, horsetail, muira puama and cat's claw are fermented with lactic acid bacteria, yeast and acetic bacteria, and the fermented material is then dried and turned into a powder).

[0044] The above test samples were subjected to a fat accumulation test according to the following procedures (a) to (L). [Fat accumulation test] (a) 37℃, 5% CO by volume 2 In the incubator, 75 cm 2 Mouse fibroblast cells 3T3-L1 were cultured in a flask in DMEM medium containing 10% (v / v) FBS. (b) Fibroblast 3TL-L1 cells were suspended by trypsinization and placed at 75 cm 2From the flask, transfer 2x10 cells to each well of a 96-well plate. 4 The cells were seeded at a density of 100 cells / well and incubated at 37°C, 5% CO 2 The cells were pre-cultured in a DMEM medium containing 10% (v / v) FBS in an incubator for 2 days. The medium was then replaced with a differentiation-inducing medium containing the test sample (control was differentiation-inducing medium only), and the cells were cultured for 3 days to induce differentiation. As the differentiation-inducing medium, a DMEM medium containing 10% (v / v) FBS containing 0.5 mM isobutylmethylxanthine, 0.5 μM dexamethasone, and 10 μg / mL insulin was used. The test samples were prepared in the differentiation-inducing medium so that the total amount of the test samples was a predetermined concentration (300 μg / mL concentration). Tables 1 and 2 show the mass ratio of each test sample to the total amount of the test samples. (c) The medium was replaced from the differentiation induction medium to a differentiation maintenance medium containing the test sample (control was differentiation maintenance medium only), and cultured for 4 days. As the differentiation maintenance medium, a 10% (v / v) FBS-containing DMEM medium containing 10 μg / mL insulin was used. The test samples were prepared in the differentiation maintenance medium so that the total amount was a specified concentration (300 μg / mL concentration). Tables 1 and 2 show the mass percentage of each test sample in the total amount of the test sample. (d) After culturing in (c), the culture medium supernatant was removed from the adipocytes. Then, 10% (v / v) formalin-containing PBS was added to the cells in an amount equal to that of the culture medium, and the cells were left to stand at room temperature for 10 minutes in the dark. The formalin solution was then removed from the cells, and the cells were washed once with PBS. (e) 10% (v / v) formalin-containing PBS was added to the cells at 100 μL / well, and the cells were then left to stand at room temperature for 10 minutes in the dark to fix the cells. (f) The formalin solution was removed, and the sections were washed twice with PBS. (g) 50 μL / well of 60% by volume isopropanol (staining solution) containing 3 mg / mL oil red was added to the adipocytes and blank wells, and the cells were stained by leaving the cells at room temperature for 30 minutes in the dark. The staining solution was then removed, and 150 μL / well of 60% by volume isopropanol aqueous solution was added to wash the cells twice. (h) 100% by volume isopropanol was added to the cells and blank wells at 100 μL / well, and the cells were shaken for about 10 minutes to extract the staining solution. The absorbance of the isopropanol solution from which the staining solution had been extracted was measured at 520 nm and 650 nm. (k) After completely removing the isopropanol solution by air drying, TM The protein amount of each cell was calculated using a BCA Protein Assay kit (Thermo Fisher Scientific). (L) The amount of fat accumulation per protein was calculated using the formula below, and the relative values ​​to the control were calculated and the results are shown in Tables 1 and 2. A smaller relative value indicates that fat accumulation in adipocytes is more suppressed. Relative fat accumulation (%) = [[(Abs520 sample - Abs520 blank) - (Abs650 sample - Abs650 blank)] / (Protein sample)] / [(Abs520 control - Abs520 blank) - (Abs650 control - Abs650 blank)] / (Protein control)]] x 100 (%) Abs520 sample, Abs650 sample: absorbance at 520 nm and 650 nm for each example or comparative example Abs520 control, Abs650 control: Absorbance of control at 520nm and 650nm Abs520 blank, Abs650 blank: Blank absorbance at 520nm and 650nm Protein sample: Amount of protein in cells in each Example or Comparative Example Protein control: The amount of protein in the cells in the control

[0045] [Table 1]

[0046] [Table 2]

[0047] It is generally known that differentiation of fibroblasts induces the expression of PPARγ, which activates fat synthesis and accumulates fat within the cells, resulting in large, round adipocytes. As shown in Table 1, the compositions of each Example, which combine digestive oligosaccharides with at least one selected from processed plant products, lactic acid bacteria, and polysaccharides, have low relative absorbance values ​​(turbidity), indicating that less fat has accumulated in fat cells. In contrast, when only digestive oligosaccharides are contained (Comparative Example 1), when only processed plant products, lactic acid bacteria, or polysaccharides are contained (Comparative Examples 2 to 18), and when trehalose is combined with processed plant products (Comparative Examples 19 to 20), the relative absorbance values ​​(turbidity) are high, indicating that more fat has accumulated in fat cells. From the above, it was shown that by combining digestible oligosaccharides with at least one selected from processed plant products, lactic acid bacteria, and polysaccharides, fat accumulation in adipocytes can be effectively suppressed and obesity can be prevented.

[0048] [Examples 21 to 39, Comparative Examples 25 to 36] Each of the above test sample powders was subjected to a myoblast activation test according to the following procedures (1) to (7). [Myoblast activation test] (1) Mouse skeletal muscle-derived myoblast cells (product name: C2C12, RIKEN BioResource Center) were incubated at 37°C and 5% CO 2 In an incubator, 75 cm 2 The cells were cultured in flasks. (2) After culturing in (1), cells were suspended by trypsinization and cultured at 75 cm 2The cells were harvested from the flask and the number of cells was counted. Then, the cells were seeded in each well of a collagen-coated 96-well plate at a cell density of 2000 cells / well together with the medium, and incubated at 37°C, 5% CO 2 The cells were pre-cultured in an incubator for 24 hours. (3) Separately from (1) and (2), the powders marked with "●" in Table 3 or Table 4 were dispersed or dissolved in 10 vol% FBS-containing DMEM medium to the concentrations shown in the left column of Table 3 or Table 4 to prepare solutions. The obtained solutions were filter-sterilized using a 0.2 μm filter (Advantec) to prepare sample solutions. However, for spore-forming lactic acid bacteria, lactic acid bacteria (killed bacteria), and bifidobacteria, the sample solutions were prepared by dispersing them. As a control, 10 vol% FBS-containing DMEM medium itself was used as the sample solution. (4) After removing the medium from each well, add 200 μL of the sample solution prepared in (3) to each well and incubate at 37°C and 5% CO 2 The cells were cultured in an incubator for 24 hours. (5) After the culture in (4), the medium was removed and each well was washed once with 200 μL / well of PBS. Then, 150 μL / well of Cell Counting Kit-8 solution (Dojindo Chemical Industries, Ltd.) diluted 30-fold with serum-free DMEM was added. (6) After adding the solution in (5), the plate was incubated at 37°C and 5% CO 2 After allowing the wells to stand in an incubator to allow for adequate color development, the absorbance of each well was measured at 450 nm. Based on the data obtained, the percentage of cells relative to the control (% of control) was calculated using the following formula, and this was taken as the myoblast activating activity. % of control=(Data sample - Data blank) / (Data control - Data blank)×100 Data sample: absorbance of each example or comparative example Data control: Control absorbance Data blank: Absorbance in the absence of cells

[0049] (7) Evaluation The calculation results of the percentage of cell counts for each Example and Comparative Example, with the control taken as 100%, are summarized in Tables 3 and 4. In Tables 3 and 4, a black circle indicates that the sample solution of the Example or Comparative Example contained the test sample in the left column at the concentration shown in the left column.

[0050] [Table 3]

[0051] [Table 4]

[0052] As shown in Table 3, the compositions of each Example, which combined digestible oligosaccharides with at least one selected from processed plant products, lactic acid bacteria, and polysaccharides, exhibited a myoblast activation effect. In contrast, as shown in Table 4, the compositions containing only digestible oligosaccharides (Comparative Examples 25 and 26), the compositions combining trehalose with processed plant products (Comparative Example 27), and the compositions containing only processed plant products, lactic acid bacteria, or polysaccharides or only polysaccharides (Comparative Examples 28 to 36) did not exhibit a myoblast activation effect. From the above, it was demonstrated that by combining digestible oligosaccharides with at least one selected from lactic acid bacteria and polysaccharides, myoblasts are effectively activated and energy metabolism in muscle tissue is enhanced.

[0053] Example 40 (Production of a composition for green juice) Raw materials were prepared according to the mixing ratios described in the following formulation examples, and a composition for green juice exhibiting the effects of the present invention was manufactured.

[0054] [Table 5]

[0055] Example 41 (Production of a composition for green juice) Raw materials were prepared according to the mixing ratios described in the following formulation examples, and a composition for green juice exhibiting the effects of the present invention was manufactured.

[0056] [Table 6]

[0057] Example 42 (Production of a composition for green juice) Raw materials were prepared according to the mixing ratios described in the following formulation examples, and a composition for green juice exhibiting the effects of the present invention was manufactured.

[0058] [Table 7]

[0059] Example 43 (Production of a composition for green juice) Raw materials were prepared according to the mixing ratios described in the following formulation examples, and a composition for green juice exhibiting the effects of the present invention was manufactured.

[0060] [Table 8]

[0061] Example 44 (Production of a composition for green juice) Raw materials were prepared according to the mixing ratios described in the following formulation examples, and a composition for green juice exhibiting the effects of the present invention was manufactured.

[0062] [Table 9]

[0063] Example 45 (Preparation of tablets) The raw materials were prepared according to the mixing ratios described in the following formulation examples, and then tablets exhibiting the effects of the present invention were produced using a tablet press.

[0064] [Table 10]

Claims

[Claim 1] The solid green juice composition contains 0.01 to 5% by mass of maltotriose and is to be dispersed or dissolved in a liquid for consumption, and further contains one or more selected from (1) to (3) described below. (1) Dried and crushed powder of one or more green leaves selected from the group consisting of young barley leaves, tencha tea, kumazasa leaves, angelica tree leaves, young sweet potato leaves, chou-meisou leaves, and mugwort leaves (2) Lactic acid bacteria (3) One or more polysaccharides selected from the group consisting of resistant dextrin, polydextrose, xanthan gum, and tamarind gum.

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