Anti-gray hair composition

Abstract

To provide a novel anti-gray hair composition.SOLUTION: Provided is an anti-gray hair composition comprising Paulownia tomentosa leaves and / or an extract thereof, and preferably further comprising Nasturtium officinale and / or an extract thereof. Preferably, the Nasturtium officinale extract is blended in the composition at a ratio of 1 / 100 to 2 relative to the Paulownia tomentosa leaf extract.SELECTED DRAWING: Figure 1

Description

[Technical field]

[0001] The present invention relates to an anti-graying composition, more specifically to a topical composition for preventing graying of hair and promoting the growth of black hair. [Background technology]

[0002] Regardless of age or gender, gray hair is one of the worries. Common measures against gray hair include plucking gray hair and dyeing hair with hair dye. In recent years, attempts have been made to prevent gray hair by using ingredients that promote melanin production in pigment cells. For example, Patent Document 1 discloses a melanin production promoter containing an extract of seaweed of the genus Porphyra in the family Bungaria. Patent Document 2 describes that melanin production activity is synergistically increased by using a combination of three ingredients, acetyl hexapeptide-1, Angelica keiskei extract, and hematin, each of which has melanin production activity.

[0003] Meanwhile, various hair compositions are provided for hair loss prevention, scalp care, etc. It is known that paulownia leaf extract and watercress extract are used as so-called hair growth ingredients in hair compositions (excluding hair dye compositions such as hair dyes). Watercress extract has the effect of suppressing the production of DKK1, a secretory protein, via MITF (transcription factor), which controls the expression of melanin biosynthesis enzyme genes (Non-Patent Document 1), and can contribute to the prevention of gray hair. However, it is not possible to fully expect a sufficient melanin production effect by simply adding watercress extract to a hair composition, and instead, there has been a demand for a plant-derived component having melanin production activity or a component that can obtain a combined effect with watercress extract. [Prior art documents] [Patent documents]

[0004] [Patent Document 1] JP 2023-82862 A [Patent Document 2] JP 2022-167819 A [Non-patent literature]

[0005] [Non-Patent Document 1] Hashimoto, Masakazu, et al., Effects of watercress extract fraction on R-spondin 1-mediated growth of human hair., International Journal of Cosmetic Science 44.2 (2022): 154-165. Summary of the Invention [Problem to be solved by the invention]

[0006] The problem to be solved by the present invention is to provide a novel melanin production promoter and an anti-hair graying composition. [Means for solving the problem]

[0007] The melanin production promoter according to the present invention contains paulownia leaf and / or paulownia leaf extract as an active ingredient. The anti-hair-graying composition according to the present invention contains paulownia leaf and / or paulownia leaf extract as an active ingredient. Furthermore, the melanin production promoter and anti-hair-graying composition according to the present invention preferably further contain watercress and / or watercress extract as active ingredients, respectively. Effect of the Invention

[0008] According to the present invention, a novel melanin production promoter and an anti-hair graying composition are provided. [Brief description of the drawings]

[0009] [Figure 1] FIG. 1 shows the enhancement of tyrosinase activity by paulownia leaf extract. [Diagram 2] FIG. 2 shows the enhancement of tyrosinase activity when paulownia leaf extract and watercress extract are used in combination. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0010] The melanin production promoter according to the present invention contains paulownia leaves and / or paulownia leaf extract as an active ingredient. Paulownia leaves are the leaves of Paulownia tomentosa, a plant belonging to the genus Paulownia in the family Thujopsis. Paulownia leaf extract is an extract obtained by extracting paulownia leaves with various extraction solvents. The extraction solvent may be any solvent generally used for plant extraction. The extraction solvent may be, for example, water, monohydric or polyhydric alcohols such as methanol, ethanol, glycerin, and esters such as acetone and ethyl acetate, or may be a hydrophilic solvent such as hexane or octanol. The extraction solvent may be one or a mixture of two or more solvents, and is preferably a hydrophilic solvent. The extraction method may be any method used for producing plant extracts. For example, the leaves are immersed in the extraction solvent, and then the leaves are removed, preferably by filtration, and then concentrated as necessary. The extraction conditions (extraction temperature, extraction time, etc.) may be appropriately determined by those skilled in the art. In the present invention, after extraction, the extract may be used as it is as the paulownia leaf extract, or may be concentrated and freeze-dried by an appropriate method and used as the paulownia leaf extract. It is also possible to use the paulownia leaf extract without removing the leaves.

[0011] The obtained paulownia leaf extract can be used as it is as a melanin production promoter. It can also be used as a melanin production promoter by mixing it with a necessary carrier as appropriate. The carrier is a component necessary for formulation, and examples of the carrier include liquids such as water and ethanol, and so-called excipients such as starch and lactose. Paulownia leaf itself can also be used as a melanin production promoter.

[0012] Furthermore, the melanin production promoter according to the present invention may contain watercress and / or watercress extract as an active ingredient. Watercress is a plant belonging to the genus Watercress of the Brassicaceae family, and is also known as watercress. Watercress extract is an extract obtained by extracting the whole plant or a part of it, such as leaves or stems, with an extraction solvent. The extraction solvent may be any solvent generally used for plant extraction. The extraction solvent may be, for example, a hydrophilic solvent such as water, monohydric or polyhydric alcohols such as methanol, ethanol, glycerin, and esters such as acetone and ethyl acetate, or a hydrophobic solvent such as hexane or octanol. The extraction solvent may be one or a mixture of two or more types, and is preferably a hydrophilic solvent. The extraction method may be any method used for producing plant extracts. For example, the watercress is produced by immersing the whole plant of watercress in the extraction solvent, removing the whole plant, preferably by filtration, and then concentrating as necessary. The extraction conditions (extraction temperature, extraction time, etc.) may be appropriately determined by those skilled in the art. In the present invention, after extraction, the extract may be used as it is as the watercress extract, or it may be concentrated and freeze-dried by an appropriate method and used as the watercress. Also, the watercress extract may be used without removing the extracted parts.

[0013] The obtained watercress extract and / or its plant body can be mixed with the paulownia leaf extract and / or paulownia leaves to produce a melanin production promoter. The paulownia leaf extract alone exhibits a melanin production promoting effect, but the melanin production promoting effect is enhanced when used in combination with watercress extract. The mixing ratio of the paulownia leaf extract and watercress extract can be appropriately determined by those skilled in the art within a range in which an enhanced effect can be obtained, but the watercress extract is generally used in an amount ratio of 1:100 to 100:100 for the paulownia leaf extract, preferably 1:100 to 2:1 for the paulownia leaf extract.

[0014] The anti-hair-graying composition according to the present invention contains the above-mentioned melanin production promoter as an active ingredient. The anti-hair-graying composition promotes melanin production to improve gray hair. The anti-hair-graying composition is mainly applied to the scalp, but can also be applied to areas where gray hair grows, such as the skin around the mouth where beard grows, arms, shins, etc.

[0015] The formulation of the anti-hair graying composition is not particularly limited as long as it is applicable to the scalp or skin other than the scalp, and may be, for example, a liquid, cream, wax, or foam. The composition may be a shampoo, rinse, treatment, hair growth agent, or any other drug, quasi-drug, or cosmetic. However, the melanin production promoter according to the present invention may be directly applied to the scalp or other skin, or may be mixed with a solvent such as water and applied to the scalp or other skin.

[0016] The content of the melanin production promoter in the anti-hair-graying composition is appropriately determined by those skilled in the art to the extent that anti-hair-graying action can be expected. The content varies depending on the dosage form and application site, but the content of paulownia leaf and / or paulownia leaf extract as the melanin production promoter is 0.0001 w / w% to 10.0 w / w% in the composition, preferably 0.001 w / w% to 2.0 w / w%.

[0017] The anti-hair-graying composition of the present invention may contain watercress and / or watercress extract as active ingredients in addition to paulownia leaf and / or paulownia leaf extract. By adding watercress extract and / or its extract, the anti-hair-graying effect is enhanced and a more effective anti-hair-graying effect is obtained.

[0018] The amount of watercress and / or watercress extract used is 1 / 100 to 100 of watercress and / or watercress extract to 1 paulownia leaf and / or paulownia leaf extract, preferably 1 / 100 to 2 of watercress extract to 1 paulownia leaf and / or paulownia leaf extract. EXAMPLES

[0019] (Tyrosinase activity enhancing effect of Paulownia leaf extract) The dried paulownia leaves were extracted with warm water, the extract was filtered to remove the leaves, and the extract was concentrated under reduced pressure to obtain the paulownia leaf extract. The tyrosinase activity, which is responsible for the reaction of melanin production, was measured for this extract.

[0020] 1.0×10 normal human melanocytes 4 The cells were seeded at 100 μL / well (96-well plate) and cultured in growth medium for 24 hours, then replaced with supplement-free medium for 24 hours. Paulownia leaf extract was added to supplement-free medium at solid extract contents of 1 μg / mL, 10 μg / mL, and 20 μg / mL, and the medium was replaced with a supplement-free medium prepared by adding the extract to the supplement-free medium, and cultured for 72 hours. 20 μL of lysis buffer was added to the 96-well plate, and the cells were dissolved by stirring with a plate mixer to obtain a crude enzyme solution, to which 100 μL / well of assay reagent (a 2:1:1 mixture of 100 mM sodium phosphate buffer, 5 mM L-dopa solution, and 20.7 mM 3-Methyl-2-benzothiazolinone hydrazone solution) was applied, and the absorbance (492 nm) was measured after 30 minutes. The results are shown in Figure 1. Paulownia leaf extract was found to have an enhancing effect on tyrosinase activity at concentrations of 10 μg / mL and 20 μg / mL, and it can be said that it has an anti-graying effect by promoting melanin production. EXAMPLES

[0021] (Tyrosinase activity enhancing effect of Paulownia leaf extract and Watercress extract) Paulownia leaf extract and watercress extract were prepared to have the solid content shown in Figure 2, and the test was carried out in the same manner as in Example 1. For reference, a hematin solution was used and the same procedure was carried out. The results are shown in Figure 2. From the results in Figure 2, when the two were used in combination, the tyrosinase activity increased from when paulownia leaf extract was used alone in the range of 1 / 100 to 2 times the watercress extract per 1 paulownia leaf extract, and it can be expected that the anti-hair graying effect can be further enhanced by using paulownia leaf extract and watercress extract in combination. On the other hand, when hematin, which is considered to be a standard substance having tyrosinase activity, was used, the enhancing effect of watercress extract was not observed, or rather, the activities of each tended to be inhibited. [Industrial Applicability]

[0022] The present invention provides a novel melanin production promoter and a novel anti-hair graying composition using the same.

Claims

1. An anti-hair graying composition comprising paulownia leaves and / or paulownia leaf extract.

2. An anti-hair graying composition further comprising watercress and / or Nasturtium officinalis extract.

3. A melanin production promoter comprising paulownia leaf and / or paulownia leaf extract.

4. A melanin production promoter further comprising watercress and / or Nasturtium officinalis extract.

5. Use of paulownia leaves and / or paulownia leaf extract on the scalp and other skin for anti-graying of hair.

6. Use of paulownia leaves and / or paulownia leaf extract for the manufacture of an anti-hair graying composition.

Images