Methods of treating erythropoietic protoporphyria, x-linked protoporphyria, or congenital erythropoietic porphyria with glycine transport inhibitors

Abstract

To provide methods of treating erythropoietic protoporphyria, X-linked protoporphyria, or congenital erythropoietic porphyria with glycine transport inhibitors.SOLUTION: The present embodiments are directed to methods of using glycine transporter inhibitors, such as GlyT1 inhibitors, or pharmaceutically acceptable salts, solvates or prodrugs thereof, or pharmaceutical compositions thereof, for preventing or treating erythropoietic protoporphyria (EPP), X-linked protoporphyria (XLPP), and / or congenital erythropoietic porphyria (CEP), and related syndromes thereof.SELECTED DRAWING: None

Description

[Technical field]

[0001] Related Applications This application claims the benefit of and priority to U.S. Provisional Patent Application No. 62 / 958,892, filed January 9, 2020, and U.S. Provisional Patent Application No. 63 / 085,942, filed September 30, 2020, which are incorporated by reference in their entireties herein.

[0002] Field Embodiments disclosed herein are directed to methods and uses for preventing or treating erythropoietic protoporphyria (EPP), X-linked protoporphyria (XLPP) or congenital erythropoietic porphyria (CEP) using a glycine transporter inhibitor, such as, but not limited to, a GlyT1 inhibitor or a pharma- ceutically acceptable salt, hydrate, prodrug, or pharmaceutical composition thereof. [Background technology]

[0003] background Erythropoietic protoporphyria (EPP) is a global disease that affects approximately 5,000–10,000 individuals worldwide (Michaels et al. 2010). It is considered the most common form of porphyria in children. Erythropoietic protoporphyria is a form of porphyria that varies in severity and can be very painful. It results from a deficiency in the enzyme ferrochelatase, resulting in abnormally high levels of protoporphyrin IX in red blood cells, plasma, skin and liver. Erythropoietic protoporphyria (EPP) is due to an inherited or acquired deficiency in the activity of the enzyme ferrochelatase. X-linked protoporphyria (XLPP) is due to an inherited increase in the activity of delta-aminolevulinic acid synthase-2 (ALAS2). The enzymes that cause both EPP and XLPP are in the heme biosynthetic pathway. EPP and XLPP are nearly identical clinically. Congenital erythropoietic porphyria (CEP), also known as Gunther's disease, is caused by a mutation in the gene for uroporphyrinogen synthase, resulting in reduced activity of this enzyme and accumulation of the upstream metabolite coproporphyrin I. Current treatments for erythropoietic protoporphyria (EPP), X-linked protoporphyria (XLPP) or congenital erythropoietic porphyria (CEP) are limited. Therefore, there is a need for new methods and compositions for treating and / or preventing erythropoietic protoporphyria, X-linked protoporphyria and congenital erythropoietic porphyria. The methods and uses of glycine transporter inhibitors, such as but not limited to GlyT1 inhibitors, as well as others, meet these needs. Summary of the Invention [Means for solving the problem]

[0004] Overview of the Application The present application provides a method of treating erythropoietic protoporphyria (EPP), X-linked protoporphyria (XLPP), or congenital erythropoietic porphyria (CEP) in a subject, comprising administering to the subject a pharmaceutical composition comprising one or more glycine transporter 1 (GlyT1) inhibitors or pharma- ceutically acceptable salts thereof, or one or more prodrugs of GlyT1 inhibitors or salts thereof.

[0005] The present application further provides a method for preventing, treating, or reducing the rate of progression and / or severity of one or more complications of EPP, XLPP or CEP in a subject, comprising administering to the subject a pharmaceutical composition comprising one or more GlyT1 inhibitors or pharma- ceutically acceptable salts thereof, or one or more prodrugs of GlyT1 inhibitors or pharma- ceutically acceptable salts thereof. In certain embodiments, the one or more complications of EPP, XLPP or CEP are selected from the group consisting of acute photosensitivity, cutaneous photosensitivity, edema, erythema, anemia, hypochromic anemia, hemolytic anemia, hemolysis, mild hemolysis, severe hemolysis, chronic hemolysis, hypersplenism, palmar keratoderma, blisters, lesions, scarring, deformity, loss of nails, loss of fingers, cholestasis, cell lysis, gallstones, cholestatic liver failure, cholelithiasis, mild liver disease, worsening liver disease, end-stage liver disease, red teeth, hyperplastic bone marrow, myelodysplasia, thrombocytopenia, fetal hydrops and / or intrauterine death. In certain such embodiments, the acute photosensitivity is due to sun exposure.

[0006] The present application further provides a method for use in the prevention or treatment of EPP, XLPP or CEP in a subject, the use comprising administering to the subject a GlyT1 inhibitor or a pharma- ceutically acceptable salt thereof, or one or more prodrugs of a GlyT1 inhibitor or a pharma- ceutically acceptable salt thereof.

[0007] The present application further provides a method for use in the manufacture of a medicament for the treatment of EPP, XLPP or CEP in a subject, the use comprising administering to the subject at least one GlyT1 inhibitor or a pharma- ceutically acceptable salt thereof, or one or more prodrugs of a GlyT1 inhibitor or a pharma- ceutically acceptable salt thereof.

[0008] The present application further provides a method for use in the manufacture of a medicament for inhibiting protoporphyrin IX (PPIX) synthesis in vivo, the use comprising administering to a subject a GlyT1 inhibitor or a pharma- ceutically acceptable salt thereof, or one or more prodrugs of a GlyT1 inhibitor or a pharma- ceutically acceptable salt thereof.

[0009] In certain embodiments, the subject has EPP. In other embodiments, the subject has XLPP. In yet other embodiments, the subject has CEP.

[0010] In certain embodiments, the method increases painless light exposure in a subject. In other embodiments, the method decreases light sensitivity in a subject.

[0011] The present application further provides a method for inhibiting PPIX synthesis in vivo, the method comprising administering to a subject a GlyT1 inhibitor or a pharma- ceutically acceptable salt thereof, or a prodrug of a GlyT1 inhibitor or a pharma- ceutically acceptable salt thereof.

[0012] The present application further provides a method for inhibiting zinc protoporphyrin IX (ZPPIX) synthesis in vivo, comprising administering to a subject a GlyT1 inhibitor or a pharma- ceutically acceptable salt thereof, or a prodrug of a GlyT1 inhibitor or a pharma- ceutically acceptable salt thereof.

[0013] The present application further provides a method for inhibiting uroporphyrin I and / or coproporphyrin I synthesis in vivo, comprising administering to a subject a GlyT1 inhibitor or a pharma- ceutically acceptable salt thereof, or a prodrug of a GlyT1 inhibitor or a pharma- ceutically acceptable salt thereof.

[0014] The present application further provides a method for inhibiting 5-aminolevulinic acid (5-ALA) synthesis in vivo, comprising administering to a subject a GlyT1 inhibitor or a pharma- ceutically acceptable salt thereof, or a prodrug of a GlyT1 inhibitor or a pharma- ceutically acceptable salt thereof.

[0015] In certain embodiments, accumulation of one or more heme intermediates is inhibited, and the one or more heme intermediates are selected from the group consisting of PPIX, ZPPIX, uroporphyrin I, coproporphyrin I, and / or 5-ALA. In certain such embodiments, accumulation of the one or more heme intermediates is inhibited in a dose-dependent manner.

[0016] In certain embodiments, the GlyT1 inhibitor demonstrates an EC50 of less than 500 nM. In certain embodiments, the GlyT1 inhibitor demonstrates an EC50 of less than 100 nM.

[0017] In certain embodiments, at least 50% cell viability is maintained, hi certain embodiments, at least 90% cell viability is maintained.

[0018] In certain embodiments, the subject has a PPIX level that is at least 10%, 20%, 30%, 40%, or 50% higher than the PPIX level in a healthy subject prior to administration of the GlyT1 inhibitor.

[0019] In certain embodiments, the subject has a ZPPIX level that is at least 10%, 20%, 30%, 40%, or 50% higher than the ZPPIX level in a healthy subject prior to administration of the GlyT1 inhibitor.

[0020] In certain embodiments, the subject has an increased ratio of ZPPIX to free protoporphyrin IX (ZPPIX / PPIX ratio) compared to a subject with EPP.

[0021] In certain embodiments, the subject has uroporphyrin I and / or coproporphyrin I levels that are at least 10%, 20%, 30%, 40% or 50% higher than the uroporphyrin I and / or coproporphyrin I levels in a healthy subject prior to administration of the GlyT1 inhibitor.

[0022] In certain embodiments, the subject has a 5-ALA level that is at least 10%, 20%, 30%, 40%, or 50% higher than the 5-ALA level in a healthy subject prior to administration of the GlyT1 inhibitor.

[0023] In certain embodiments, the PPIX level of the subject is reduced, while the heme level of the patient is substantially maintained.In certain embodiments, the PPIX level of the patient is reduced by at least 50% (e.g., 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or at least 100%), and the heme level of the patient is not reduced by more than 10% (e.g., 10%, 15%, 20%, 25% and 30%).In certain embodiments, the PPIX level of the patient is reduced by at least 85% and the heme level of the patient is not reduced by more than 15%.In certain embodiments, the heme level is not reduced by more than 10% (e.g., 10%, 15%, 20%, 25% and 30%).In certain embodiments, the administration of the pharmaceutical composition does not cause a substantial reduction in the heme level.

[0024] In certain embodiments, the subject has an increased free protoporphyrin IX level in red blood cells. In certain embodiments, the method reduces the free protoporphyrin IX level in the subject. In certain such embodiments, the method reduces the free protoporphyrin IX level in the subject by at least 10% (e.g., 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or at least 100%). In certain embodiments, the subject has an increased protoporphyrin IX level in feces. In certain embodiments, the method reduces the protoporphyrin IX level in the feces of the subject. In certain such embodiments, the method reduces protoporphyrin IX levels in the subject's feces by at least 10% (e.g., 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or at least 100%).

[0025] In certain embodiments, the subject's plasma porphyrins fluoresce with a peak of 634 nm when irradiated with blue light (e.g., 400-420 nm light). In certain embodiments, the subject's plasma porphyrins fluoresce with a peak of 626 nm to 634 nm when irradiated with blue light (e.g., 400-420 nm light). In certain embodiments, the subject's skin porphyrins fluoresce with a peak of 632 nm when irradiated with blue light (e.g., 400-420 nm light). In certain embodiments, the subject's skin porphyrins fluoresce with a peak of 626 nm to 634 nm when irradiated with blue light (e.g., 400-420 nm light).

[0026] In certain embodiments, the subject has an increased protoporphyrin IX level in the skin. In certain embodiments, the method reduces the protoporphyrin IX level in the skin of the subject. In certain such embodiments, the method reduces the protoporphyrin IX level in the skin of the subject by at least 10% (e.g., 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or at least 100%). In certain embodiments, the subject has a protoporphyrin IX level in the skin greater than 0.2 FluoDerm Units (FDU). In certain embodiments, the subject has a protoporphyrin IX level in the skin greater than 1.0 FDU. In certain embodiments, the subject has a protoporphyrin IX level in the skin between 1.0 FDU and 2.5 FDU. In certain embodiments, the subject has a protoporphyrin IX level in the skin that is higher than 2.5 FDU. In certain embodiments, the method reduces the protoporphyrin IX level in the skin of the subject to less than 0.5 FDU. In certain embodiments, the method reduces the protoporphyrin IX level in the skin of the subject to less than 1.0 FDU. In certain embodiments, the method reduces the protoporphyrin IX level in the skin of the subject to less than 1.5 FDU. In certain embodiments, the method reduces the protoporphyrin IX level in the skin of the subject to less than 2.0 FDU. In certain embodiments, the method reduces the protoporphyrin IX level in the skin of the subject to less than 2.5 FDU.

[0027] In certain embodiments, the subject has an increased protoporphyrin IX level in red blood cells. In certain embodiments, the method reduces the protoporphyrin IX level in the red blood cells of the subject. In certain such embodiments, the method reduces the protoporphyrin IX level in the red blood cells of the subject by at least 10% (e.g., 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or at least 100%). In certain embodiments, the subject is administered 31 μmol L -1 In certain embodiments, the subject has a protoporphyrin IX level in red blood cells that is greater than 31 μmol L -1 ~53μmolL -1 In certain embodiments, the subject has a protoporphyrin IX level in red blood cells of 53 μmol L -1 In certain embodiments, the method comprises increasing the protoporphyrin IX level in the red blood cells of the subject to 53 μmol L -1 In certain embodiments, the method reduces protoporphyrin IX levels in red blood cells of the subject to less than 31 μmol L -1 In certain embodiments, the method reduces protoporphyrin IX levels in red blood cells of the subject to less than 15 μmol L -1 Reduce to a level below.

[0028] In certain embodiments, the subject's ferrocheletase activity level is reduced to 10-35% of the ferrocheletase activity level observed in a normal subject. In certain embodiments, the subject's ferrocheletase activity level is reduced to less than 50% of the ferrocheletase activity level observed in a normal subject.

[0029] In certain embodiments, the subject has a gain-of-function mutation in ALAS2. In certain embodiments, the subject's ALAS2 enzymatic activity is increased.

[0030] In certain embodiments, the subject has an increased zinc protoporphyrin IX level in red blood cells. In certain embodiments, the method reduces the zinc protoporphyrin IX level in the red blood cells of the subject. In certain such embodiments, the method reduces the zinc protoporphyrin IX level in the red blood cells of the subject by at least 10% (e.g., 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or at least 100%).

[0031] In certain embodiments, the subject has a decreased activity of uroporphyrinogen III synthase. In certain embodiments, the subject has an increased level of uroporphyrin I and / or coproporphyrin I. In certain embodiments, the increased level of uroporphyrin I and / or coproporphyrin I is measured in the urine or red blood cells of the subject. In certain embodiments, the increased level of coproporphyrin I is measured in the feces of the subject. In certain embodiments, the method reduces the level of uroporphyrin I and / or coproporphyrin I of the subject. In certain embodiments, the method reduces the level of uroporphyrin I of the subject. In certain such embodiments, the method reduces the subject's level of uroporphyrin I by at least 10% (e.g., 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or at least 100%). In certain embodiments, the method reduces the subject's level of coproporphyrin I. In certain such embodiments, the method reduces the subject's level of coproporphyrin I by at least 10% (e.g., 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or at least 100%).

[0032] In certain embodiments, the subject has a mutation in UROS.

[0033] In certain embodiments, the subject has a genetic defect in the GATA-1 erythroid-specific transcription factor.

[0034] In certain embodiments, the subject has red fluorescent urine, hi certain embodiments, the subject has a peak at 615 nm to 620 nm using plasma porphyrin fluorescence analysis.

[0035] In certain embodiments, the subject has liver disease associated with EPP, XLPP or CEP.In certain embodiments, the liver disease associated with EPP, XLPP or CEP is cholelithiasis.In certain embodiments, the liver disease associated with EPP, XLPP or CEP is mild liver disease.In certain embodiments, the liver disease associated with EPP, XLPP or CEP is exacerbation of liver disease.In certain embodiments, the liver disease associated with EPP, XLPP or CEP is end-stage liver disease.

[0036] In certain embodiments, the method further comprises administering to the subject an additional active agent and / or supportive therapy. In certain such embodiments, the additional active agent and / or supportive therapy is selected from the group consisting of sun avoidance, topical sunscreens, skin protection, UVB phototherapy, afamelanotide (Scenesse®), bortezomib, proteasome inhibitors, chemical chaperones, cholestyramine, activated charcoal, iron supplementation, liver transplant, bone marrow transplant, splenectomy, and blood transfusion.

[0037] In certain embodiments, the GlyT1 inhibitor is [ka] [wherein Ar is an unsubstituted or substituted aryl or 6-membered heteroaryl containing 1, 2 or 3 nitrogen atoms, where the substituted aryl and heteroaryl groups are hydroxy, halogen, NO 2 ,CN,(C1 ~C 6 )-alkyl, halogen-substituted (C 1 ~C 6 )-alkyl, hydroxy substituted (C 1 ~C 6 )-alkyl, (CH 2 )n-(C 1 ~C 6 )-alkoxy, halogen-substituted (C 1 ~C 6 )-Alkoxy, NR 7 R 8 , C(O)R 9 , SO2R 10 and -C(CH 3 )=NOR 7 or (C 1 ~C 6 )-substituted by a 5-membered aromatic heterocycle containing 1 to 4 heteroatoms selected from N and O, optionally substituted by alkyl; R 1 is hydrogen or (C 1 ~C 6 )-alkyl; R 2 is hydrogen, (C 1 ~C 6 )-alkyl, (C 2 ~C 6 )-alkenyl, halogen-substituted (C 1 ~C 6 )-alkyl, hydroxy substituted (C 1 ~C 6 )-alkyl, (C 1 ~C 6 )-alkoxy or halogen optionally substituted (CH2)n-(C 3 ~C 7 )-cycloalkyl, CH(CH 3 )-(C 3 ~C 7 )-cycloalkyl, (CH 2 ) n+1 -C(O)-R 9 , (CH 2 ) n+1-CN, bicyclo[2.2.1]heptyl, (CH 2 ) n+1 -O-(C 1 ~C 6 )-alkyl, (CH 2 ) n -heterocycloalkyl, (CH 2 ) n -aryl or (CH 2 ) n - 5- or 6-membered heteroaryl containing 1, 2 or 3 heteroatoms selected from the group consisting of oxygen, sulfur or nitrogen (CH 2 ) n - 5- or 6-membered heteroaryl, where aryl, heterocycloalkyl and heteroaryl are unsubstituted or substituted with hydroxy, halogen, (C 1 ~C 6 )-Alkyl and (C 1 ~C 6 )-alkoxy; R 3 , R 4 and R 6 are each independently hydrogen, hydroxy, halogen, (C 1 ~C 6 )-alkyl, (C 1 ~C 6 )-alkoxy or O-(C 3 ~C 6 )-cycloalkyl; R 5 No 2 , C.N., C(O)R. 9 or SO 2 R 10 ;R 7 and R 8 are each independently hydrogen or (C1-C6)-alkyl; R 9 is hydrogen, (C 1 ~C 6 )-alkyl, (C 1 ~C 6 )-alkoxy or NR 7 R 8 ;R 10 is optionally substituted with halogen (C1 ~C 6 )-alkyl, (CH 2 ) n -(C 3 ~C 6 )-cycloalkyl, (CH 2 ) n -(C 3 ~C 6 )-alkoxy, (CH 2 ) n -heterocycloalkyl or NR 7 R 8 and n is 0, 1, or 2. or a pharma- ceutically acceptable salt thereof, or a prodrug of the compound or a pharma- ceutically acceptable salt thereof.

[0038] In certain embodiments, the GlyT1 inhibitor is [ka] or a pharma- ceutically acceptable salt thereof, or a prodrug of the compound or a pharma- ceutically acceptable salt thereof.

[0039] In certain embodiments, the GlyT1 inhibitor is [ka] [In the formula, R 1 represents a heteroaryl selected from the group consisting of imidazolyl, thiazolyl, pyridyl, oxazolyl, pyrazolyl, triazolyl, oxadiazolyl, quinolinyl, isoxazolyl, pyrrolyl, and thiadiazole, wherein said heteroaryl is selected from the group consisting of -OH, -NR 7 R 8 , halogens, (C 1 ~C 8 ) alkyl, (C 3 ~C 10 ) cycloalkyl, (C 1 ~C 8 ) alkoxy, (C 1 ~C 12 ) alkoxyalkyl, (C1 ~C 8 ) hydroxyalkyl, (C 6 ~C 14 ) optionally substituted with one or more substituents selected from aryl and benzyl; R 2 , R 3 and A is independently H or (C 1 ~C 8 )alkoxy, where the alkyl is selected from one or more -OH, (C 1 ~C 8 ) Alkoxy, -NR 7 R 8 or halogen; Q is -(CH 2 ) n -(wherein n=1, 2, 3 or 4) or -(CH 2 ) m -O- (wherein m=2, 3 or 4); Z represents (C 6 ~C 14 ) aryl, (C 1 ~C 8 ) alkyl or (C 3 ~C 8 ) represents cycloalkyl; R 4 and R 5 are each independently H, halogen, (C 1 ~C 8 ) alkyl, (C 6 ~C 14 ) aryl, (C 6 ~C 14 )aryloxy, (C 1 ~C 8 )alkoxy, (3-10 membered)heterocycloalkyl or (C 3 ~C 8 ) cycloalkoxy; where R 4 and R 5 is one or more -OH, (C 1 ~C 8 )Alkoxy, -NR 7 R 8 or halogen; Y is -R 6 , -(CH2 )oR 6 , -C(R 6 ) 3 or -CH(R 6 ) 2 (wherein 0=1, 2 or 3); R 6 is H, (C 6 ~C 14 ) aryl, (C 1 ~ 10 ) alkyl, (C 3 ~C 10 ) cycloalkyl, (C 5 ~C 18 ) bicycloalkyl, (C 5 ~C 18 )tricycloalkyl, (3-10 membered)heterocycloalkyl, (5-10 membered)heteroaryl, -C(=O)NR 7 R 8 OR -C(=O)OR 7 (Wherein, R 6 The group may be optionally substituted by one or more X groups; X=-OH, (C 1 ~C 8 ) Alkoxy, -NR 11 R 12 , -SO 2 R 10 , -C(=O)R 10 , halogen, cyano, (C 1 ~C 8 ) alkyl, (C 1 ~C 10 )alkoxyalkyl, (5-10 membered)heteroaryl, (C 6 ~C 14 ) aryl, (C 6 ~C 14 )aryloxy, benzyl or (C1-C 8 ) hydroxyalkyl; R 7 and R 8 are independently H, (C 1 ~C 8 ) alkyl, (C 3 ~C8) cycloalkyl, (5-10 membered) heterocycloalkyl, (C 1 ~C 8) hydroxyalkyl, (5-10 membered) heteroaryl or (C 1 ~C 10 ) alkoxyalkyl; R 7 and R 8 is optionally substituted with one or more X groups; or R 7 and R 8 may, together with the nitrogen to which they may be attached, form a (3-10 membered) heterocycloalkyl group optionally substituted with one or more X groups; R 10 is (C 1 ~C 8 ) alkyl, (C 3 ~C 8 )cycloalkyl, (3-10 membered)heterocycloalkyl, (C 1 ~C 8 ) hydroxyalkyl, (5-10 membered) heteroaryl or (C 1 ~C 10 ) alkoxyalkyl; R 11 and R 12 are independently H, (C 1 ~C 8 ) alkyl, (C 3 ~C 8 )cycloalkyl, (5-10 membered)heterocycloalkyl, (C 1 ~C 8 ) hydroxyalkyl, (5-10 membered) heteroaryl or (C 1 ~C 10 ) represents alkoxyalkyl] or a pharma- ceutically acceptable salt thereof, or a prodrug of the compound or a pharma- ceutically acceptable salt thereof. In certain such embodiments, the GlyT1 inhibitor is [ka] or a pharma- ceutically acceptable salt thereof, or a prodrug of the compound or a pharma- ceutically acceptable salt thereof. In other such embodiments, the GlyT1 inhibitor is a compound having the formula: [ka] or a pharma- ceutically acceptable salt thereof, or a prodrug of the compound or a pharma- ceutically acceptable salt thereof.

[0040] In certain embodiments, the GlyT1 inhibitor is [ka] [In the formula, Z 1 is C 1~4 Alkyl, C 3~6 Cycloalkyl, C 1~4 Alkoxy, C 1~4 Alkylthio, HaloC 1~4 Alkyl, phenyl, haloC 1~4 Alkoxy, halophenyl, C 1~4 Alkyl sulfoxy, C 1~4 selected from the group consisting of alkylsulfonyl, bromo and chloro; Z 2 is hydrogen, halogen, cyano, C 1~4 Alkyl, phenyl, haloC 1~4 Alkyl, haloC 1~4 Alkoxy, halophenyl, C 1~4 Alkoxy C 1~4 Alkyl and C 3~6 cycloalkyl; Z 3 is hydrogen, halogen, C 1~4 Alkyl, C 1~4 Alkoxy, C 1~4 Alkylthio, HaloC 1~4 Alkyl, haloC 1~4 Alkoxy and C 3~6 cycloalkyl; Z 4 is hydrogen, halogen, C1-3 alkyl, haloC 1~4 Alkyl, C 1~4 Alkoxy, C 1~4 Alkylthio, Phenyl, HaloC 1~4 Alkoxy, halophenyl, C 1~4 Alkoxy C 1~4 Alkyl and C 3~6cycloalkyl; Z 5 is hydrogen, fluoro, chloro, bromo, iodo, hydroxy, C 1~4 Alkyl, C 1~4 Alkoxy, C 1~4 Alkylthio, Phenyl, HaloC 1~4 Alkyl, haloC 1~4 Alkoxy, halophenyl, C 1~4 Alkoxy C 1~4 Alkyl and C 3~6 cycloalkyl; 1 ~Z 5 When more than one of Z is methoxy, 1 and Z 5 Only R is methoxy. 3 and R 4 are independently selected from hydrogen and C optionally substituted with one or more groups Y. 1~4 alkyl; or R 3 and R4, together with the nitrogen atom to which they are attached, form a saturated or partially unsaturated 5-, 6- or 7-membered carbocyclic ring optionally substituted with a group Y'; Y is C 1~4 Alkoxy, Hydroxy, HaloC 1~4 Alkoxy and C 3~5 cycloalkyl; Y' is selected from the group consisting of C 1~4 Alkyl, C 1~4 Alkoxy, halogen, hydroxy, haloC 1~4 Alkoxy, C 3~5 Cycloalkyl and C 5~10 aryl, or Y' forms a -CH2- or -CH2-CH2- bridge between two atoms on A, a 5-, 6- or 7-membered carbocyclic ring; R 5 and R 6 is independently a C optionally substituted with one or more groups X; 1~4 alkyl; or R 5 and R 6 together with the carbon atoms to which they are attached form a saturated 5- or 6-membered carbocyclic ring optionally substituted with one or more groups X'; R5 and R6 together with the carbon atom to which they are attached form a 5-membered saturated carbocyclic ring, which ring may optionally further contain a group of additional heteroatoms selected from O, N and S(O)m, where m=0, 1 or 2; X is halogen, hydroxy, C 1~4 Alkoxy, HaloC 1~4 Alkyl, haloC 1~4 Alkoxy and C 5~10 aryl; X' is selected from the group consisting of halogen, hydroxy, C 1~4 Alkyl, C 1~4 Alkoxy, HaloC 1~4 Alkyl, haloC 1~4 Alkoxy and C 5~10 aryl; where R 3 , R 4 , R 5 and R 6 are not all simultaneously unsubstituted methyl; however, at the same time, Z 1 is propyloxy, and Z 3 is chloro and Z 2 =Z 4 =Z 5 =H and R 5 and R 6 If both are methyl, R 3 and R 4 do not form a 2-methylpyrrolidine group together with the nitrogen atom to which they are attached; 1 is methyl and Z 3 is methoxy and Z 2 = Z4 = Z5 = H, and R 5 and R 6 If both are methyl, R 3 and R 4 do not form, together with the nitrogen atom to which they are attached, a pyrrolidine group. or a pharma- ceutically acceptable salt thereof, or a prodrug of the compound or a pharma- ceutically acceptable salt thereof. In certain such embodiments, the GlyT1 inhibitor is [ka] or a pharma- ceutically acceptable salt thereof, or a prodrug of the compound or a pharma- ceutically acceptable salt thereof.

[0041] In certain embodiments, the GlyT1 inhibitor is [ka] [Wherein, Z is (CH 2 ) n , O, S, SO, SO 2 or NR 5 n is 0, 1 or 2; X is hydrogen, halogen, (C 1~6 ) alkyloxy, (C 3~6 ) cycloalkyloxy, (C 6~12 )aryloxy, (C 6~12 ) Aryl, Thienyl, SR 6 , SOR 6 , S.O. 2 R 6 , N.R. 6 R 6 , N.H.R. 6 , N.H. 2 , N.H.C.O.R. 6 , NSO 2 R 6 , CN, COOR 6 , and halogens, (C 6~12 ) aryl, (C 1~6 ) alkyloxy or (C 6~12 ) optionally substituted with aryloxy (C 1~4 ) alkyl; or two substituents at adjacent positions together represent a fused (C 5~6 ) aryl group, fused (C 5~6 ) cycloalkyl ring or O-(CH 2 ) m m is 1 or 2; Y is hydrogen, halogen, (C 1~4 ) Alkyloxy, SR 6 , N.R. 6 R 6 , and optionally substituted with halogen (C 1~4) 1 to 3 substituents independently selected from alkyl; R 1 COOR 7 or CONR 8 R 9 ;R 2 and R6 is (C 1~4 ) alkyl; R 3 , R 4 and R 5 are independently hydrogen or (C 1~4 ) alkyl; R 7 , R 8 and R 9 are independently hydrogen, (C 1~4 ) alkyl, (C 6~12 ) aryl or arylalkyl] or a pharma- ceutically acceptable salt thereof, or a prodrug of the compound or a pharma- ceutically acceptable salt thereof. In certain such embodiments, the GlyT1 inhibitor is [ka] or a pharma- ceutically acceptable salt thereof, or a prodrug of the compound or a pharma- ceutically acceptable salt thereof.

[0042] In certain embodiments, the GlyT1 inhibitor is [ka] [In the formula, n is an integer of 1 to 3; R 1 and R 2 is independently selected from hydrogen, alkyl, haloalkyl, alkoxy, haloalkoxy, aryl, heteroaryl, cycloalkyl, or heterocyclyl, wherein said rings are independently selected from alkyl, halo, haloalkyl, alkoxy, haloalkoxy, hydroxy, cyano, monosubstituted amino, or disubstituted amino; a , R b or R c or R 1 and R 2when attached to the same carbon atom, can be combined to form a cycloalkyl or a monocyclic saturated heterocyclyl to give a spirocycle, where the cycloalkyl or monocyclic saturated heterocyclyl is independently selected from alkyl, alkoxy, fluoro, fluoroalkyl, fluoroalkoxy, hydroxy, monosubstituted amino, or disubstituted amino. d , R c or R f or R 1 and R 2 When attached to the 2- and 5- or 3- and 6-carbon atoms of a piperazine ring, they combine to form -C 1 ~C 3 - an alkylene chain, in which one of the carbon atoms in the alkylene chain is optionally replaced by -NR-, -O-, -S(O)n- (wherein R is hydrogen or alkyl and n is 0 to 2), and further, one or two hydrogen atoms in the alkylene chain can be optionally replaced by one or two alkyl; R 3 , R 4 and R 5 are independently hydrogen, alkyl, fluoro or fluoroalkyl; Ar 1 and Ar 2 is independently aryl, heteroaryl, cycloalkyl, or heterocyclyl, where each of the foregoing rings is selected from R g , R h or R i and R g is alkyl, -C=CR 6 (In the formula, R 6 is aryl or heteroaryl), halo, haloalkyl, haloalkoxy, alkylthio, cyano, alkoxy, amino, monosubstituted amino, disubstituted amino, sulfonyl, acyl, carboxy, alkoxycarbonyl, hydroxyalkyl, alkoxyalkyl, aminoalkyl, hydroxyalkoxy, alkoxyalkoxy, aminoalkoxy, aminosulfonyl, aminocarbonyl, or acylamino; R h and R iis independently selected from alkyl, halo, haloalkyl, haloalkoxy, alkylthio, cyano, alkoxy, amino, monosubstituted amino, disubstituted amino, sulfonyl, acyl, carboxy, alkoxycarbonyl, hydroxyalkyl, alkoxyalkyl, aminoalkyl, hydroxyalkoxy, alkoxyalkoxy, aminoalkoxy, aminosulfonyl, aminocarbonyl, acylamino, aryl, heteroaryl, cycloalkyl, or heterocyclyl, where R g , R h and R i The aromatic ring or alicyclic ring in the formula (I) is selected from the group consisting of alkyl, halo, haloalkyl, haloalkoxy, alkylthio, cyano, alkoxy, amino, monosubstituted amino, disubstituted amino, sulfonyl, acyl, carboxy (carbpxy), alkoxycarbonyl, hydroxyalkyl, alkoxyalkyl, aminoalkyl, and the like. R is independently selected from aryl, hydroxyalkoxy, alkoxyalkoxy, aminoalkoxy, aminosulfonyl, aminocarbonyl, or acylamino; j , R k or R l with the proviso that the compound of formula V is not 2-(4-benzhydrylpiperazin-l-yl)acetic acid, 2-(4-((4-chlorophenyl)(phenyl)methyl)piperazin-l-yl)acetic acid, 2-((2R,5S)-4-((R)-(4-(lH-tetrazol-5-yl)phenyl)(3-hydroxyphenyl)methyl)-2,5-dimethylpiperazin-l-yl)acetic acid or 2-((2R,5S)-4-((R)-(4-cyanophenyl)(3-hydroxyphenyl)methyl)-2,5-dimethylpiperazin-l-yl)acetic acid. or a pharma- ceutically acceptable salt thereof, or a prodrug of the compound or a pharma- ceutically acceptable salt thereof. In certain such embodiments, the GlyT1 inhibitor is [ka] or a pharma- ceutically acceptable salt thereof, or a prodrug of the compound or a pharma- ceutically acceptable salt thereof.

[0043] In certain embodiments, the GlyT1 inhibitor is [ka]

[0023] In the formula, A is a compound represented by the general formula NR 1 Groups of the general formula N+(O−)R 1 or a group of the general formula N+(R')R 1 (wherein R 1 is a straight-chain or branched (C 1 ~C 7 ) alkyl group, or (C 4 ~C 7 ) a cycloalkyl group, or (C 3 ~C 7 )Cycloalkyl(C 1 ~C 3 ) alkyl group, or phenyl optionally substituted with one or two hydroxyl or methoxy groups (C 1 ~C 3 ) alkyl group, or (C 2 ~C 4 ) an alkenyl group, or (C 2 ~C 4 R' represents either a linear or branched (C 1 ~C 7 ) alkyl group; X is a hydrogen atom, or a halogen atom and trifluoromethyl, linear or branched (C1-C4) alkyl and (C 1 ~C 4 ) one or more substituents selected from alkoxy groups; R 2 is a hydrogen atom, or a halogen atom and trifluoromethyl, (C 1 ~C 4 ) alkyl group or (C 1 ~C 4 ) an alkoxy group, or a group of the general formula NR 3 R 4 (wherein R 3 and R 4 are each independently a hydrogen atom or (C1 ~C 4 ) an alkyl group or, together with the nitrogen atom bearing them, a pyrrolidine ring, a piperidine ring or a morpholine ring, or a phenyl group optionally substituted with atoms or groups as defined above for the symbol X), or a pharma- ceutically acceptable salt thereof, or a prodrug of the compound or a pharma- ceutically acceptable salt thereof. In certain such embodiments, the GlyT1 inhibitor is [ka] or a pharma- ceutically acceptable salt thereof, or a prodrug of the compound or a pharma- ceutically acceptable salt thereof.

[0044] In certain embodiments, the GlyT1 inhibitor is [ka] [In the formula, R 1 is -(CH 2 ) n -R 1a (In the formula, n is independently 0 to 6; R 1a (1) C which is unsubstituted or substituted with 1 to 6 halogens or hydroxyl 1~6 Alkyl, (2) R 2a , R 2b and R 2c (3) unsubstituted or substituted phenyl 1~6 Alkyl, 1-6 halogens, hydroxy or -NR 10 R 11 C replaced with 3~6 cycloaryl, (4) unsubstituted or substituted with 1 to 6 halogen, hydroxy or -NR 10 R 11 -OC is substituted with 1~6 Alkyl, (5)-COR 9(wherein R9 is (a) hydrogen, (b) unsubstituted or substituted with 1 to 6 fluoro -C 1~6 (c) alkyl, (d) benzyl, and (e) phenyl; (6)-NR 10 R 11 (In the formula, R 10 and R 11 is (a) hydrogen, (b) unsubstituted or is selected from hydroxy, 1 to 6 fluoro, or -NR 12 R 13 (In the formula, R 12 and R 13 are independently hydrogen and -C 1~6 alkyl) 1~6 alkyl, (c) unsubstituted or selected from hydroxy, 1 to 6 fluoro, or -NR 12 R 13 -C is substituted with 3~6 (c) -CH 2 ... 10 R 11 R2 is selected from the group consisting of (1) R 2a , R 2b and R 2c (2) phenyl that is unsubstituted or substituted with 1 to 6 halogen, hydroxy, -NR 10 R 11 , C substituted with phenyl or heterocycle 1~8 Alkyl (wherein phenyl or heterocycle is R 2a , R 2b and R 2c (3) unsubstituted or substituted with 1 to 6 halogen, hydroxy or -NR 10 R 11 C replaced with 3~6 cycloalkyl, and (4) unsubstituted or substituted with 1 to 6 halogen, hydroxy, or -NR 10 R 11 -C is substituted with 1~6 Alkyl-(C 3~6 cycloalkyl); R 2a , R2b and R 2c is (1) hydrogen, (2) halogen, (3) unsubstituted or (a) 1 to 6 halogens, (b) phenyl, (c) C 3~6 Cycloalkyl, or (d) -NR 10 R 11 -C is substituted with 1~6 alkyl, (4) -OC which is unsubstituted or substituted with 1 to 6 halogens 1~6 Alkyl, (5) Hydroxy, (6) -SCF 3 , (7)-SCHF 2 , (8)-S.C.H. 3 , (9)-CO 2 R 9 , (10)-CN, (11)-SO 2 R 9 , (12)-SO 2 -NR 10 R 11 , (13)-NR 10 R 11 , (14)-CONR 10 R 11 , and (15)-NO 2 R 3 (1) is unsubstituted or contains 1 to 6 halogen, hydroxyl, or -NR 10 R 11 C replaced with 1~6 (2) unsubstituted or containing 1 to 6 halogens, hydroxyl, or -NR 10 R 11 C replaced with 3~6 cycloalkyl; R 4 and R 5 is (1) hydrogen, and (2) C which is unsubstituted or substituted with halogen or hydroxyl. 1~6 alkyl, or R 4 and R 5 Together, C 3~6 forms a cycloalkyl ring; A is (1) -O-, and (2) -NR 10-; m is zero or 1, where when m is zero, R 2 directly bonds to the carbonyl. and pharma- ceutically acceptable salts thereof, as well as individual enantiomers and diastereomers thereof or pharma- ceutically acceptable salts thereof, or a prodrug of the compound or a pharma- ceutically acceptable salt thereof. In certain such embodiments, the GlyT1 inhibitor is [ka] or a pharma- ceutically acceptable salt thereof, or a prodrug of the compound or a pharma- ceutically acceptable salt thereof.

[0045] In certain embodiments, the GlyT1 inhibitor is [ka] [In the formula, R 1 is a halogen, C 1 ~C 3 Alkyl, C 3 ~C 6 Cycloalkyl, OR 9 or S.R. 10 phenyl independently substituted 1 to 5 times with, 1 ~C 3 Alkyl and C 3 ~C 6 Cycloalkyl is R 7 substituted 1 to 10 times as necessary; R 2 is H;R 3 and R4 are each independently H or CH 3 ;R 5 are (1) hydrogen, (2) R 7 C optionally substituted 1 to 11 times with 1 ~C 6 (3) gem-dialkyl, and (4) gem-dihalo; or two R 5 The substituents, together with the carbon atom to which they are attached, form R 7or two R on adjacent carbons of the ring to which they are attached form a 3-, 4- or 5-membered cycloalkyl optionally substituted 1 to 10 times with 5 The substituents, taken together, are R 7 R 6 teeth, [ka] (wherein E, F, and G are each independently nitrogen or carbon; R 6a is C optionally substituted 1 to 5 times with halogen or deuterium 1 ~C 2 alkyl); R 7 is (1) hydrogen, (2) halogen, (3) deuterium, (4) gem-dialkyl, (5) gem-dihalo, (6) -OR 9 , -NR 11 R 12 , -NR 11 C(O) p R 10 , -S(O) p R 10 , -CN, -NO2, -C(O) p R 10 , -C(O)NR 11 R 12 or NR 11 C(S)R 10 and (7) selected from the group consisting of oxo or thio; R 8 are (1) hydrogen, (2) halogen, and (3) C 1 ~C 6 Alkyl, C 2 ~C 6 Alkenyl, C 2 ~C 6 Alkynyl, C 3 ~C 7 Cycloalkyl or C 4 ~C 7 Cycloalkylalkyl (where C 1 ~C 6 Alkyl, C 2 ~C 6 Alkenyl, C 2 ~C6 Alkynyl, C 3 ~C 7 Cycloalkyl and C 4 ~C 7 Each cycloalkylalkyl is independently R 7 substituted 1 to 11 times as needed), or (4)-OR 9 , -NR 11 R 12 , -NR 11 C(O) p R 10 , -S(O) p R 10 , -CN, -NO2, -C(O) p R 10 , -C(O)NR 11 R 12 Or -NR 11 C(S)R 10 R 9 is hydrogen, C 1 ~C 4 Alkyl, C 3 ~C 7 Cycloalkyl, C 4 ~C 7 Cycloalkylalkyl, -C(O)NR 11 R 12 and -C(O) p R 10 wherein C is selected from the group consisting of 1 ~C 4 Alkyl, C 3 ~C 7 Cycloalkyl and C 4 ~C 7 Each of the cycloalkylalkyls is R 7 substituted 1 to 11 times as necessary; R 10 is hydrogen, C 1 ~C 4 Alkyl, C 3 ~C 7 Cycloalkyl, C 4 ~C 7 is selected from the group consisting of cycloalkyl, alkyl, aryl and heteroaryl, 1 ~C 4 Alkyl, C 3 ~C 7Cycloalkyl and C 4 ~C 7 Each cycloalkylalkyl is optionally substituted 1 to 11 times with a substituent defined in R7, and an aryl or heteroaryl is optionally substituted 1 to 11 times with a substituent defined in R 8 is substituted 1 to 10 times as necessary; R 11 and R 12 is hydrogen, C 1 ~C 4 Alkyl, C 3 ~C 7 Cycloalkyl, C 4 ~C 7 each independently selected from the group consisting of cycloalkyl, alkyl, aryl, and heteroaryl, 1 ~C 4 Alkyl, C 3 ~C 7 Cycloalkyl and C 4 ~C 7 Each of the cycloalkylalkyls is R 7 and the aryl or heteroaryl is optionally substituted 1 to 11 times with a substituent as defined in 8 or R 11 and R 12 together with the nitrogen to which they are attached, R 7 A forms a saturated or partially saturated monocyclic or fused bicyclic heterocycle optionally substituted 1 to 11 times with [ka] X is N; Y is N; p is 1 or 2; m is 0; provided that R 6 cannot be (a) 1H-1,2,3-triazol-4-yl, or (b) 5-methylisoxazol-4-yl. or an oxide thereof, a pharma- ceutically acceptable salt of the compound or the oxide thereof, or an individual enantiomer or diastereomer thereof.

[0046] In certain embodiments, the GlyT1 inhibitor is [ka] [ka] [ka] or a pharma- ceutically acceptable salt thereof, or a prodrug of the compound or a pharma- ceutically acceptable salt thereof.

[0047] In certain embodiments, the GlyT1 inhibitor is represented by formula IX [ka] [In the formula, R 1 represents phenyl or a 5- or 6-membered monocyclic heteroaryl having 1, 2 or 3 heteroatoms independently selected from O, N or S, where the phenyl or heteroaryl is optionally joined to one or more R 3 with R being substituted as necessary; 2 represents an aryl, a 5- or 6-membered monocyclic heteroaryl, or an 8-10-membered bicyclic heteroaryl, where the monocyclic or bicyclic heteroaryl has 1, 2, or 3 heteroatoms independently selected from O, N, or S, and where the aryl or heteroaryl has one or more R 4 with R being substituted as necessary; 3 is a halogen, C 1~4 -Alkyl or C 3~6 -cycloalkyl, where C 1~4 -Alkyl or C 3~6 -cycloalkyl is optionally substituted with one or more halogens; R 4 is halogen, -CN, C 1~4 -Alkyl, C 3~6 -cycloalkyl, -C 1~3 -Alkyl-C 3~6 -Cycloalkyl or -OC 1~6 alkyl, where C1~4 -Alkyl, C 3~6 -cycloalkyl, -C 1~3 -Alkyl-C 3~6 -Cycloalkyl or -OC 1~6 -alkyl is optionally substituted with one or more halogens. or a pharma- ceutically acceptable salt thereof, or a tautomer or stereoisomer of the compound or a pharma- ceutically acceptable salt thereof, or a mixture of any of the foregoing.

[0048] In certain embodiments, the GlyT1 inhibitor has the formula X [ka] [In the formula, R 1 is a) a 5- or 6-membered monocyclic heteroaryl having 1, 2, 3, or 4 heteroatoms independently selected from the group consisting of O, N, and S(O)r; b) a 5- or 6-membered monocyclic partially saturated heterocycloalkyl having 1, 2, or 3 heteroatoms independently selected from the group consisting of O, N, and S(O)r; and c) a 5- or 6-membered monocyclic partially saturated heterocycloalkyl having 1, 2, or 3 heteroatoms independently selected from the group consisting of O, N, and S(O)r. r wherein each of said groups a), b) and c) is selected from the group consisting of: C 1~4 -Alkyl-, C 1~4 -Alkyl-O-, oxetanyl, tetrahydrofuranyl, tetrahydropyranyl, C 3~6 -cycloalkyl- and C 3~6 -cycloalkyl-O-, which in the case of a substituent is attached to the nitrogen ring atom, and said substituent is 1~4 -Alkyl-, C 1~4 -Alkyl-CO-, C 3~6 -cycloalkyl- and C 3~6 -cycloalkyl-CO-, wherein C 1~4 -Alkyl-, C1~4 -Alkyl-O-, C 1~4 -Alkyl-CO-, oxetanyl, tetrahydrofuranyl, tetrahydropyranyl, C 3~6 -cycloalkyl-, C 3~6 -Cycloalkyl-CO- or C 3~6 Each of the -cycloalkyl-O- substituents is fluoro, -CF 3 , -CHF 2 , -CH 2 may be substituted by one or more substituents independently selected from the group consisting of F and -CN; R 2 is hydrogen, C 1~4 -Alkyl-, C 1~4 -Alkyl-O-, -CN and C 3~6 -cycloalkyl-, wherein C 1~4 -Alkyl-, C 1~4 -Alkyl-O- and C 3~6 Each -cycloalkyl- group is fluoro, -CF 3 , -CHF 2 , -CH 2 optionally substituted with one, two, three or more substituents independently selected from the group consisting of F and -CN; R 3 is C 1~6 -Alkyl-O-, C 3~6 -cycloalkyl-O-, morpholino, pyrazolyl, and one oxygen atom and optionally O, N and S(O) as ring members s (wherein s=0, 1, or 2), wherein the C 1~6 -alkyl-O- and the C 3~6 -Cycloalkyl-O- is fluoro, -CF 3 , -CHF 2 , -CH 2 F, -CN, C 1~4 -Alkyl-, C 3~6 -cycloalkyl-, C 1~6 -Alkyl-O- and C 3~6-cycloalkyl-O-, optionally substituted with one, two, three or more substituents independently selected from the group consisting of: R 4 is hydrogen; or R 3 and R 4 together with the ring atoms of the phenyl group to which they are attached, represent 4-, 5-, or 6-membered monocyclic partially saturated heterocycloalkyl, or each of which is O, N, and S(O) s (wherein s=0, 1 or 2), wherein in general formula (I), R 3 There must be one ring oxygen atom directly attached to the ring carbon atom of said phenyl group to which is attached; wherein said heterocycloalkyl group is fluoro, -CF 3 , -CHF 2 , -CH 2 F, -CN, C 1~4 -Alkyl-, C 3~6 -cycloalkyl-, C 1~6 -Alkyl-O-, C 3~6 - optionally substituted with one, two, three or more substituents independently selected from the group consisting of cycloalkyl-O-, oxetanyl-O-, tetrahydrofuranyl-O- and tetrahydropyranyl-O-; R 5 is hydrogen; R 6 is hydrogen, C 1~4 -Alkyl-SO 2 -, C 3~6 -Cycloalkyl-SO 2 and -CN; R 7 is hydrogen; or a) R 6 and R 7 or b) R 6 and R 5 The first pair of phenyl groups, together with the ring atoms of the phenyl groups to which they are attached, are O, N and S(O) u(wherein u=0, 1 or 2), wherein in general formula (I), R 6 one -SO bonded directly to the ring carbon atom of the phenyl group to which is bonded 2 -member must be present, where the heterocycloalkyl group is fluoro, -CF 3 , -CHF 2 , -CH 2 F, -CN, C 1~4 -Alkyl-, C 1~6 -Alkyl-O- and C 3~6 -cycloalkyl-O-, optionally substituted with 1, 2, 3 or more substituents independently selected from the group consisting of:

[0049] In certain embodiments, the pharmaceutical composition further comprises a pharma- ceutically acceptable carrier.

[0050] In certain embodiments, the subject is a subject in need thereof.

[0051] In certain embodiments, the GlyT1 inhibitor or a pharma- ceutically acceptable salt thereof, or a prodrug of a GlyT1 inhibitor or a pharma- ceutically acceptable salt thereof, is administered in a therapeutically effective amount. [Brief description of the drawings]

[0052] [Figure 1] FIG. 1 shows Western blot determination of ferrochelatase (FECH) protein expression levels for various K562 clones.

[0053] [Diagram 2] FIG. 2 shows flow cytometric determination of protoporphyrin IX (PPIX) levels for K562 clones.

[0054] [Diagram 3] FIG. 3 shows heme and PPIX levels in WT K562 and clone 1-9 cells as determined by LC / MS / MS.

[0055] [Figure 4] FIG. 4 shows the effect of bitopertin and PF-03463275 on PPIX levels as determined by flow cytometry.

[0056] [Diagram 5] FIG. 5 shows the effect of bitopertin and PF-03463275 on cell viability as measured by the Vi-CELL XR complete system.

[0057] [Figure 6] FIG. 6 shows the effect of bitopertin treatment on 5-aminolevulinic acid (5-ALA) in clone 1-9 cells.

[0058] [Figure 7] FIG. 7 shows the effect of bitopertin treatment on PPIX levels in clone 1-9 cells.

[0059] [Figure 8] FIG. 8 shows the effect of bitopertin treatment on heme levels in clone 1-9 cells.

[0060] [Figure 9] FIG. 9 shows relative FECH mRNA levels in human hematopoietic stem cells following transduction with lentiviral vectors expressing shRNA for FECH.

[0061] [Figure 10] FIG. 10 shows flow cytometric determination of the effect of bitopertin treatment on red blood cell antigen profile and protoporphyrin IX (PPIX) levels in human hematopoietic stem cells.

[0062] [Figure 11] FIG. 11 shows that Biotopertin (100 nM) treatment reduced PPIX accumulation by 60%. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0063] Detailed Description of the Application Unless otherwise defined, all technical and scientific terms have the same meaning as commonly understood by one of ordinary skill in the art to which the disclosed embodiments belong.

[0064] As used herein, the terms "a" or "an" mean "at least one" or "one or more than one," unless the context clearly dictates otherwise.

[0065] As used herein, the term "about" means that the numerical values ​​are approximations and small variations that will not significantly affect the practice of the disclosed embodiments. When numerical limitations are used, unless otherwise indicated by context, "about" means that the numerical values ​​can vary by ±10% and still be within the scope of the disclosed embodiments.

[0066] The term "acyl" is art-recognized and refers to a group represented by the general formula hydrocarbylC(O)-, preferably alkylC(O)-.

[0067] As used herein, the term "acylamino" refers to an amino group substituted with an acyl group (e.g., -OC(=O)-H or -OC(=O)-alkyl). Examples of acylamino are -NHC(=O)H or -NHC(=O)CH. 3 The term "lower acylamino" refers to a lower acyl group (e.g., -OC(=O)-H or -OC(=O)-C 1~6 An example of a lower acylamino is -NHC(=O)H or -NHC(=O)CH 3 It is.

[0068] The term "acyloxy" is art-recognized and refers to a group represented by the general formula hydrocarbylC(O)O-, preferably alkylC(O)O-.

[0069] As used herein, the term "alkenyl" means a straight or branched alkyl group having one or more double carbon-carbon bonds and 2 to 20 carbon atoms, including, but not limited to, ethenyl, 1-propenyl, 2-propenyl, 2-methyl-1-propenyl, 1-butenyl, 2-butenyl, etc. In some embodiments, the alkenyl chain is 2 to 10 carbon atoms in length, 2 to 8 carbon atoms in length, 2 to 6 carbon atoms in length, or 2 to 4 carbon atoms in length.

[0070] The terms "alkoxy", "phenyloxy", "benzoxy" and "pyrimidinyloxy" refer to an optionally substituted alkyl, phenyl, benzyl or pyrimidinyl group, respectively, attached through an oxygen atom. For example, the term "alkoxy" refers to a straight or branched -O-alkyl group of 1-20 carbon atoms, including but not limited to methoxy, ethoxy, n-propoxy, isopropoxy, t-butoxy, and the like. In some embodiments, the alkoxy chain is 1-10 carbon atoms long, 1-8 carbon atoms long, 1-6 carbon atoms long, 1-4 carbon atoms long, 2-10 carbon atoms long, 2-8 carbon atoms long, 2-6 carbon atoms long, or 2-4 carbon atoms long.

[0071] As used herein, the term "alkyl" refers to a linear or branched saturated hydrocarbon group. The alkyl group can contain 1-20, 2-20, 1-10, 2-10, 1-8, 2-8, 1-6, 2-6, 1-4, 2-4, 1-3, or 2 or 3 carbon atoms. Examples of alkyl groups include, but are not limited to, methyl (Me), ethyl (Et), propyl (e.g., n-propyl and isopropyl), butyl (e.g., n-butyl, t-butyl, isobutyl), pentyl (e.g., n-pentyl, isopentyl, neopentyl), hexyl, isohexyl, heptyl, 4,4-dimethylpentyl, octyl, 2,2,4-trimethylpentyl, nonyl, decyl, undecyl, dodecyl, 2-methylpentyl, methyl, ethyl ... 1-propyl, 2-methyl-2-propyl, 2-methyl-1-butyl, 3-methyl-1-butyl, 2-methyl-3-butyl, 2-methyl-1-pentyl, 2,2-dimethyl-1-propyl, 3-methyl-1-pentyl, 4-methyl-1-pentyl, 2-methyl-2-pentyl, 3-methyl-2-pentyl, 4-methyl-2-pentyl, 2,2-dimethyl-1-butyl, 3,3-dimethyl-1-butyl, 2-ethyl-1-butyl, and the like.

[0072] As used herein, the term "alkylamino" refers to an amino group substituted with an alkyl group having 1 to 6 carbon atoms. An example of an alkylamino is -NHCH 2 CH 3 It is.

[0073] As used herein, the term "alkylene" or "alkylenyl" refers to a divalent alkyl linking group. Examples of alkylene (or alkylenyl) are methylene or methyleneyl (-CH 2 -).

[0074] As used herein, the term "alkylthio" refers to an -S-alkyl group having 1 to 6 carbon atoms. An example of an alkylthio group is -SCH 2 CH 3 It is.

[0075] As used herein, the term "alkynyl" means a straight or branched alkyl group having one or more triple carbon-carbon bonds and 2-20 carbon atoms, including, but not limited to, acetylene, 1-propylene, 2-propylene, etc. In some embodiments, the alkynyl chain is 2-10 carbon atoms in length, 2-8 carbon atoms in length, 2-6 carbon atoms in length, or 2-4 carbon atoms in length.

[0076] The term "amide" as used herein refers to the group [ka] (Wherein, each R 30 independently represent hydrogen or a hydrocarbyl group, or two R 30 together with the N atom to which they are attached complete a heterocycle having 4 to 8 atoms in the ring structure.

[0077] As used herein, the term "amidino" refers to -C(=NH)NH 2 means... The terms "amine" and "amino" are art-recognized and refer to both unsubstituted and substituted amines, as well as their salts, e.g., [ka] (Wherein, each R 30 independently represent hydrogen or a hydrocarbyl group, or two R 30 refer to a moiety that can be represented by the formula: (which, together with the N atom to which they are attached, complete a heterocycle having 4 to 8 atoms in the ring structure).

[0078] As used herein, the term "aminoalkoxy" refers to an alkoxy group substituted with an amino group. An example of an aminoalkoxy is -OCH 2 CH 2 NH 2 It is.

[0079] As used herein, the term "aminoalkyl" refers to an alkyl group substituted with an amino group. An example of an aminoalkyl is -CH 2 CH 2 NH 2 It is.

[0080] As used herein, the term "aminosulfonyl" refers to -S(=O) 2 NH 2 means...

[0081] As used herein, the term "aminoalkylthio" refers to an alkylthio group substituted with an amino group. An example of an aminoalkylthio is -SCH 2 CH 2 NH 2 It is.

[0082] As used herein, the term "amphiphilic" refers to a three-dimensional structure that has distinct hydrophobic and hydrophilic regions. An amphiphilic compound preferably has the presence of both hydrophobic and hydrophilic elements.

[0083] As used herein, the term "animal" includes, but is not limited to, humans and non-human vertebrate animals, such as wild animals, domestic animals and farm animals.

[0084] As used herein, the term "aryl" refers to a monocyclic, bicyclic or polycyclic (e.g., having 2, 3 or 4 fused rings) aromatic hydrocarbon. In some embodiments, an aryl group has 6 to 20 carbon atoms, or 6 to 10 carbon atoms. Examples of aryl groups include, but are not limited to, phenyl, naphthyl, anthracenyl, phenanthrenyl, indanyl, indenyl, tetrahydronaphthyl, and the like. Examples of aryl groups include, but are not limited to, [ka] [ka] Examples include:

[0085] As used herein, the term "arylalkyl" refers to a C substituted with aryl. 1~6 It means alkyl.

[0086] As used herein, the term "arylamino" refers to an amino group substituted with an aryl group. An example of an arylamino is -NH (phenyl).

[0087] As used herein, the term "arylene" refers to an aryl linking group, ie, an aryl group that links one group to another group in a molecule.

[0088] The term "carbamate" is art-recognized and refers to a group [ka] (In the formula, R 29 and R 30 independently represent hydrogen or a hydrocarbyl group, e.g., an alkyl group, or R 29 and R 30 which, together with the intervening atoms, complete a heterocycle having 4 to 8 atoms in the ring structure.

[0089] As used herein, the term "carbamoyl" refers to -C(=O)NH 2 means...

[0090] As used herein, the term "carbocycle" means a 5- or 6-membered saturated or unsaturated cyclic ring optionally containing an O, S or N atom as part of the ring. Examples of carbocycles include, but are not limited to, cyclopentyl, cyclohexyl, cyclopenta-1,3-diene, phenyl, and any of the heterocycles listed above.

[0091] The term "carbocyclylalkyl," as used herein, refers to an alkyl group substituted with a carbocycle group.

[0092] The term "carbonate" is art-recognized and refers to the group -OCO 2 -R 30 (In the formula, R 30 represents a hydrocarbyl group).

[0093] The term "carboxy" as used herein refers to a group of the formula -CO 2 H.

[0094] As used herein, the term "carrier" refers to a diluent, adjuvant or excipient that is administered with a compound. Pharmaceutical carriers can be liquids, such as water and oils, including those of petroleum, animal, vegetable or synthetic origin, such as peanut oil, soybean oil, mineral oil, sesame oil and the like. Pharmaceutical carriers can also be saline, gum acacia, gelatin, starch paste, talc, keratin, colloidal silica, urea and the like. In addition, auxiliary agents, stabilizers, thickeners, lubricants and colorants can be used.

[0095] As used herein, the term "compound" refers to all stereoisomers, tautomers, and isotopes of the compounds described herein.

[0096] As used herein, the terms "comprising" (and any form of comprising, such as "comprise," "comprises," and "comprised"), "having" (and any form of having, such as "have" and "has"), "including" (and any form of including, such as "includes" and "include"), or "containing" (and any form of containing, such as "contains" and "contain") are inclusive or open-ended and do not exclude additional, unrecited elements or method steps.

[0097] As used herein, the term "contacting" refers to bringing two elements together in an in vitro or in vivo system. For example, "contacting" a GlyT1 transporter inhibitor with a GlyT1 transporter in an individual or patient or cell includes not only administration of the compound to an individual or patient, e.g., a human, but also includes introducing the compound into a sample containing a cell preparation or purified preparation that contains the GlyT1 transporter.

[0098] As used herein, the term "cyano" means --CN.

[0099] As used herein, the term "cycloalkyl" means a non-aromatic cyclic hydrocarbon, including cyclized alkyl, alkenyl, and alkynyl groups containing up to 20 ring-forming carbon atoms. Cycloalkyl groups can include monocyclic or polycyclic ring systems, such as fused, bridged, and spiro ring systems. In some embodiments, polycyclic ring systems contain 2, 3, or 4 fused rings. Cycloalkyl groups can contain 3 to 15, 3 to 10, 3 to 8, 3 to 6, 4 to 6, 3 to 5, or 5 or 6 ring-forming carbon atoms. Ring-forming carbon atoms of a cycloalkyl group can be optionally substituted by oxo or sulfido. Examples of cycloalkyl groups include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, cyclononyl, cyclopentenyl, cyclohexenyl, cyclohexadienyl, cycloheptatrienyl, norbornyl, norpinyl, norcarnyl, adamantyl, and the like. Also included in the definition of cycloalkyl are moieties having one or more aromatic rings fused (having a bond in common with the cycloalkyl ring) to the cycloalkyl ring, e.g., benzo or thienyl derivatives of pentane, pentene, hexane, etc. (e.g., 2,3-dihydro-1H-inden-1-yl or 1H-inden-2(3H)-on-1-yl).

[0100] As used herein, the term "cycloalkylalkyl" refers to a C substituted with cycloalkyl. 1~6 It means alkyl.

[0101] As used herein, the term "dialkylamino" means an amino group substituted with two alkyl groups each having 1 to 6 carbon atoms.

[0102] As used herein, the term "diazamino" refers to -N(NH 2 ) 2 means...

[0103] The term "ester" as used herein refers to the group -C(O)OR 30 (In the formula, R 30 represents a hydrocarbyl group).

[0104] The term "ether" as used herein refers to a hydrocarbyl group linked to another hydrocarbyl group through an oxygen. Thus, the ether substituent of a hydrocarbyl group can be hydrocarbyl-O-. Ethers can be either symmetrical or asymmetrical. Examples of ethers include, but are not limited to, heterocycle-O-heterocycle and aryl-O-heterocycle. Ethers include "alkoxyalkyl" groups, which can be represented by the general formula alkyl-O-alkyl.

[0105] As used herein, the term "facially amphiphilic" or "facially amphiphilic" refers to a compound having polar and nonpolar side chains that adopt a conformation that results in the separation of the polar (hydrophilic) and nonpolar (hydrophobic) side chains on opposite faces or separate regions of the structure or molecule.

[0106] As used herein, the term "glycine transporter" or "GlyT" refers to a membrane protein that facilitates the transport of glycine across the plasma membrane of a cell. Non-limiting examples of glycine transporters include glycine transporter 1 (GlyT1) and glycine transporter 2 (GlyT2).

[0107] As used herein, the term "GlyT1" or "GlyT1 transporter" refers to sodium- and chloride-dependent glycine transporter 1, also known as glycine transporter 1, which in humans is a protein encoded by the SLC6A9 gene (Kim KM, Kingsmore SF, Han H, Yang-Feng TL, Godinot N, Seldin MF, Caron MG, Giros B (Jun 1994). "Cloning of the human glycine transporter type 1: molecular and pharmacological characterization of novel isoform variants and chromosomal localization of the gene in the human and mouse genomes". Mol Pharmacol. 45 (4): 608-17;Jones EM, Fernald A, Bell GI, Le Beau MM (Nov 1995). "Assignment of SLC6A9 to human chromosome band 1p33 by in situ hybridization". Cytogenet Cell Genet. 71 (3): 211), which are incorporated herein by reference in their entireties.

[0108] As used herein, the term "GlyT2" or "GlyT2 transporter" refers to the sodium- and chloride-dependent glycine transporter 2, also known as glycine transporter 2, which in humans is a protein encoded by the SLC6A5 gene (Morrow JA, Collie IT, Dunbar DR, Walker GB, Shahid M, Hill DR (November 1998). "Molecular cloning and functional expression of the human glycine transporter GlyT2 and chromosomal localisation of the gene in the human genome". FEBS Lett. 439 (3): 334-40), which is incorporated by reference in its entirety. and is hereby incorporated by reference.

[0109] As used herein, the term "GlyT1 inhibitor" refers to a compound that inhibits or blocks the activity of the GlyT1 transporter, including compounds that inhibit the activity of any isoform of GlyT1. Non-limiting examples of GlyT1 inhibitors are provided herein. In some embodiments, the GlyT1 inhibitor is a specific GlyT1 inhibitor, meaning that the inhibitor has a higher inhibitory activity against GlyT1 compared to GlyT2. In some embodiments, the inhibitor inhibits GlyT1 with at least 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, 99% selectivity or about 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, 99% selectivity compared to GlyT2. In some embodiments, the GlyT1 inhibitor inhibits GlyT1 but does not inhibit or does not significantly inhibit the activity of GlyT2. A GlyT1 inhibitor that does not significantly inhibit the activity of GlyT2 then inhibits the activity of GlyT2 by less than 5%, 4%, 3%, 2% or 1%. The selectivity of a GlyT1 inhibitor can be determined by assays known in the art, for example, by the published journal articles (BN Atkinson, SC Bell, M. De Vivo, LR Kowalski, SM Lechner, VI Ognyanov, C.-S. Tham, C. Tsai, J. Jia, D. Ashton and MA Klitenick, ALX 5407: A Potent, Selective Inhibitor of the hGlyT1 Glycine Transporter, Molecular Pharmacology December 2001, 60 (6) 1414-1420), which is incorporated herein in its entirety.

[0110] As used herein, the term "GlyT2 inhibitor" refers to a compound that inhibits or blocks the activity of the GlyT2 transporter, including compounds that inhibit the activity of any isoform of GlyT2. In some embodiments, the GlyT2 inhibitor is a non-specific inhibitor, meaning that it can also inhibit or block the activity of GlyT1. In some embodiments, the GlyT2 inhibitor is a specific GlyT2 inhibitor, meaning that it has a higher inhibitory activity against GlyT2 compared to GlyT1. In some embodiments, the inhibitor inhibits GlyT2 with at least 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, 99% selectivity or with about 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, 99% selectivity compared to GlyT1. In some embodiments, the GlyT2 inhibitor inhibits GlyT2 activity but does not inhibit or does not significantly inhibit the activity of GlyT1. A GlyT2 inhibitor that does not significantly inhibit the activity of GlyT1 then inhibits the activity of GlyT1 by less than 5%, 4%, 3%, 2% or 1%. The selectivity of GlyT2 inhibitors can be determined using assays known in the art, for example, in published journal articles (BN Atkinson, SC Bell, M. De Vivo, LR Kowalski, SM Lechner, VI Ognyanov, C.-S. Tham, C. Tsai, J. Jia, D. Ashton and MA Klitenick, ALX 5407: A Potent, Selective Inhibitor of the hGlyT1 Glycine Transporter, Molecular Pharmacology December 2001, 60 (6) 1414-1420), which is incorporated herein in its entirety.

[0111] As used herein, the term "guanidino" refers to -NH(=NH)NH2 means...

[0112] As used herein, the term "halo" means a halogen radical, including, but not limited to, fluoro, chloro, bromo and iodo.

[0113] As used herein, the term "haloalkoxy" refers to an -O-haloalkyl group. An example of a haloalkoxy group is OCF 3 It is.

[0114] As used herein, the term "haloalkyl" refers to a C alkyl group having one or more halogen substituents. 1~6 An example of a haloalkyl group is, but is not limited to, CF 3 , C 2 F 5 , C.H. 2 F, CHF 2 , CCl 3 , CHCl 2 , C.H. 2 CF 3 etc.

[0115] As used herein, the term "heteroaryl" refers to an aromatic heterocycle having up to 20 ring-forming atoms (e.g., C) and at least one heteroatom ring member (ring-forming atom) such as sulfur, oxygen, or nitrogen. In some embodiments, a heteroaryl group has at least one or more heteroatom ring-forming atoms, each of which is independently sulfur, oxygen, or nitrogen. In some embodiments, a heteroaryl group has 3 to 20 ring-forming atoms, 3 to 10 ring-forming atoms, 3 to 6 ring-forming atoms, or 3 to 5 ring-forming atoms. In some embodiments, a heteroaryl group contains 2 to 14 carbon atoms, 2 to 7 carbon atoms, or 5 or 6 carbon atoms. In some embodiments, a heteroaryl group has 1 to 4 heteroatoms, 1 to 3 heteroatoms, or 1 or 2 heteroatoms. Heteroaryl groups include monocyclic and polycyclic (e.g., having 2, 3, or 4 fused rings) systems. Examples of heteroaryl groups include, but are not limited to, pyridyl, pyrimidinyl, pyrazinyl, pyridazinyl, triazinyl, furyl, quinolyl, isoquinolyl, thienyl, imidazolyl, thiazolyl, indolyl (such as indol-3-yl), pyrroyl, and oxazolyl. , benzofuryl, benzothienyl, benzthiazolyl, isoxazolyl, pyrazolyl, triazolyl, tetrazolyl, indazolyl, 1,2,4-thiadiazolyl, isothiazolyl, benzothienyl, purinyl, carbazolyl, benzimidazolyl, indolinyl, pyranyl, oxadiazolyl, isoxazolyl, triazolyl, thianthrenyl, pyrazolyl, indolizinyl, isoindolyl, isobenzofuranyl, benzoxazolyl, xanthenyl, 2H-pyrrolyl, pyrrolyl, 3H-indolyl, 4H-quinolizinyl, phthalazinyl, naphthyridinyl, quinazolinyl, phenanthridinyl, acridinyl, perimidinyl, phenanthrolinyl, phenazinyl, isothiazolyl, phenothiazinyl, isoxazolyl, furanyl, and phenoxazinyl groups. Suitable heteroaryl groups include 1,2,3-triazole, 1,2,4-triazole, 5-amino-1,2,4-triazole, imidazole, oxazole, isoxazole, 1,2,3-oxadiazole, 1,2,4-oxadiazole, 3-amino-1,2,4-oxadiazole, 1,2,5-oxadiazole, 1,3,4-oxadiazole, pyridine and 2-aminopyridine.

[0116] As used herein, the term "heteroarylalkyl" refers to a C substituted with a heteroaryl group. 1~6 It means an alkyl group.

[0117] As used herein, the term "heteroarylamino" refers to an amino group substituted with a heteroaryl group. An example of a heteroarylamino is -NH-(2-pyridyl).

[0118] As used herein, the term "heteroarylene" means a heteroaryl linking group, ie, a heteroaryl group that links one group to another group in a molecule.

[0119] The term "heteroatom" as used herein means an atom of any element other than carbon or hydrogen. Exemplary heteroatoms are nitrogen, oxygen, and sulfur.

[0120] As used herein, the term "heterocycle" or "heterocyclic ring" refers to a 5-7 membered monocyclic or bicyclic or 7-10 membered bicyclic heterocyclic ring system, any of whose rings may be saturated or unsaturated, consisting of carbon atoms and 1-3 heteroatoms selected from N, O and S, where the N and S heteroatoms may be optionally oxidized, and the N heteroatom may be optionally quaternized, including any bicyclic group in which any of the heterocyclic rings defined above are fused to a benzene ring. Particularly useful are rings containing one oxygen or sulfur, one to three nitrogen atoms, or one oxygen or sulfur in combination with one or two nitrogen atoms. The heterocyclic ring may be attached at any heteroatom or carbon atom that results in the creation of a stable structure. Examples of heterocyclic groups include, but are not limited to, piperidinyl, piperazinyl, 2-oxopiperazinyl, 2-oxopiperidinyl, 2-oxopyrrolodinyl, , 2-oxoazepinyl, azepinyl, pyrrolyl, 4-piperidonyl, pyrrolidinyl, pyrazolyl, pyrazolidinyl, imidazolyl, imidazolinyl, imidazolidinyl, pyridyl, pyrazinyl, pyrimidinyl, pyridazinyl, oxazolyl, oxazolidinyl, isoxazolyl, isoxazolidinyl, morpholinyl, thiazolyl, thiazolidinyl, isothiazolyl, quinuclidinyl, isothiazolidinyl, indolyl, quinolinyl, isoquinolinyl, benzimidazolyl, thiadiazoyl, benzopyranyl, benzothiazolyl, benzoxazolyl, furyl, tetrahydrofuryl, tetrahydropyranyl, thienyl, benzothienyl, thiamorpholinyl, thiamorpholinyl sulfoxide, thiamorpholinyl sulfone and oxadiazolyl. Morpholino is the same as morpholinyl.

[0121] As used herein, the term "heterocycloalkyl" means a non-aromatic heterocycle having up to 20 ring-forming atoms, including cyclized alkyl, alkenyl, and alkynyl groups in which one or more ring-forming carbon atoms are replaced by a heteroatom, such as an O, N, or S atom. Heterocycloalkyl groups can be monocyclic or polycyclic (e.g., fused, bridged, or spiro) systems. In some embodiments, the heterocycloalkyl group has 1 to 20 carbon atoms, or 3 to 20 carbon atoms. In some embodiments, the heterocycloalkyl group contains 3 to 14 ring-forming atoms, 3 to 7 ring-forming atoms, or 5 or 6 ring-forming atoms. In some embodiments, the heterocycloalkyl group has 1 to 4 heteroatoms, 1 to 3 heteroatoms, or 1 or 2 heteroatoms. In some embodiments, the heterocycloalkyl group contains 0 to 3 double bonds. In some embodiments, the heterocycloalkyl group contains 0 to 2 triple bonds. Examples of heterocycloalkyl groups include, but are not limited to, morpholino, thiomorpholino, piperazinyl, tetrahydrofuranyl, tetrahydrothienyl, 2,3-dihydrobenzofuryl, 1,3-benzodioxole, benzo-1,4-dioxane, piperidinyl, pyrrolidinyl, isoxazolidinyl, oxazolidinyl, isothiazolidinyl, pyrazolidinyl, thiazolidinyl, imidazolidinyl, pyrrolidin-2-one-3-yl, and the like. In addition, ring-forming carbon atoms and heteroatoms of a heterocycloalkyl group can be optionally substituted with oxo or sulfide. For example, ring-forming S atoms can be substituted with one or two oxos (S(O) or S(O)). 2For another example, ring-forming C atoms can be substituted by oxo (forming carbonyl). Also included in the definition of heterocycloalkyl are moieties having one or more aromatic rings fused to a non-aromatic heterocyclic ring (having a common bond with the non-aromatic heterocyclic ring), including, but not limited to, pyridinyl, thiophenyl, phthalimidyl, naphthalimidyl and benzo derivatives of heterocycles such as indolene, isoindolene, 4,5,6,7-tetrahydrothieno[2,3-c]pyridin-5-yl, 5,6-dihydrothieno[2,3-c]pyridin-7(4H)-one-5-yl, isoindolin-1-one-3-yl and 3,4-dihydroisoquinolin-1(2H)-one-3yl groups. Ring-forming carbon atoms and heteroatoms of heterocycloalkyl groups can be optionally substituted by oxo or sulfido.

[0122] As used herein, the term "heterocycloalkylalkyl" refers to a C substituted with heterocycloalkyl. 1~6 Refers to alkyl.

[0123] As used herein, the term "hydroxy" or "hydroxyl" means an --OH group.

[0124] As used herein, the term "hydroxyalkyl" or "hydroxylalkyl" refers to an alkyl group substituted with a hydroxyl group. Examples of hydroxylalkyl include, but are not limited to, -CH 2 OH and -CH 2 CH 2 OH is an example.

[0125] As used herein, the terms "individual" or "patient", used interchangeably, refer to any animal, including mammals, such as mice, rats, other rodents, rabbits, dogs, cats, pigs, cows, sheep, horses, or primates, such as humans.

[0126] As used herein, the phrase "inhibitory activity," eg, of an enzyme or transporter activity, means reducing the activity of an enzyme or transporter, eg, a GlyT1 transporter, by any measurable amount.

[0127] As used herein, the phrase "in need thereof" means that an animal or mammal has been identified as having a need for a particular method or treatment. In some embodiments, the identification can be by any means of diagnosis. The animal or mammal may be in need of any of the methods and treatments described herein. In some embodiments, the animal or mammal is in or progressing to an environment where a particular disease, disorder or condition is prevalent.

[0128] As used herein, the phrase "capable of gelling in situ" is meant to encompass not only low viscosity liquids that form gels upon contact with the eye or lacrimal fluid outside the eye, but also more viscous liquids, such as semi-fluids and thixotropic gels, that exhibit a substantial increase in viscosity or gel consistency upon administration to the eye.

[0129] As used herein, the phrase "an integer between X and Y" refers to any integer, including the endpoints. For example, the phrase "an integer between X and Y" refers to 1, 2, 3, 4, or 5.

[0130] The term "lower" when used in conjunction with chemical moieties such as acyl, acyloxy, alkyl, alkenyl, alkynyl, or alkoxy, is meant to include groups in which there are 10 or fewer non-hydrogen atoms, preferably 6 or fewer non-hydrogen atoms, in the substituent. "Lower alkyl" refers, for example, to alkyl groups containing 10 or fewer carbon atoms, preferably 6 or fewer carbon atoms. In certain embodiments, acyl, acyloxy, alkyl, alkenyl, alkynyl, or alkoxy substituents as defined herein are lower acyl, lower acyloxy, lower alkyl, lower alkenyl, lower alkynyl, or lower alkoxy, respectively, whether they occur alone or in combination with other substituents, such as hydroxyalkyl and aralkyl (in which case, for example, atoms in aryl groups are not counted when counting carbon atoms in an alkyl substituent) in the description.

[0131] As used herein, the term "mammal" means a rodent (i.e., a mouse, rat, or guinea pig), monkey, cat, dog, cow, horse, pig, or human. In some embodiments, the mammal is a human.

[0132] As used herein, the term "N-alkyl" refers to an alkyl chain substituted with an amine group. Non-limiting examples include, but are not limited to: [ka] and the like. The alkyl chain can be linear, branched, cyclic, or any combination thereof. In some embodiments, the alkyl contains 1-10, 1-9, 1-8, 1-7, 1-6, 1-5, 1-4, 1-3, or 1-2 carbons.

[0133] As used herein, the term "nitro" refers to -NO 2 means...

[0134] As used herein, the term "n-membered" (where n is an integer) typically describes the number of ring-forming atoms in a moiety, where the number of ring-forming atoms is n. For example, pyridine is an example of a 6-membered heteroaryl ring, and thiophene is an example of a 5-membered heteroaryl ring.

[0135] As used herein, the phrase "ophthalmically acceptable" means having no lasting adverse effect on the treated eye or its function, or on the overall health of the treated subject. However, it will be recognized that transient effects, such as mild irritation or a "stinging" sensation, are common with topical administration of drugs, and the presence of such transient effects is not inconsistent with the composition, formulation, or ingredients (e.g., excipients) in the discussion of "ophthalmically acceptable" as defined herein.

[0136] As used herein, the phrase "optionally substituted" means that the substituent is optional, and thus includes both unsubstituted and substituted atoms and moieties. A "substituted" atom or moiety indicates that any hydrogen on the specified atom or moiety can be replaced with a selection from the indicated substituents, provided that the normal valence of the specified atom or moiety is not exceeded and the replacement results in a stable compound. For example, when a methyl group is optionally substituted, three hydrogen atoms on the carbon atom can be replaced with a substituent.

[0137] As used herein, the phrase "pharmacologically acceptable" means compounds, materials, compositions and / or dosage forms that, within the scope of sound medical judgment, are suitable for use in contact with the tissues of humans and animals. In some embodiments, "pharmacologically acceptable" means approved by a federal or state government regulatory agency or listed by the United States Pharmacopoeia or other generally recognized pharmacopoeias for use in animals, and more specifically, in humans.

[0138] "Pharmaceutically acceptable salts" is intended to mean salts of free acids or free bases of the compounds represented herein that are non-toxic, biologically tolerable, or otherwise biologically suitable for administration to a subject. Generally, see SM Berge, et al., "Pharmaceutical See, "Pharmaceutically Acceptable Salts," J. Pharm. Sci., 1977, 66, 1-19. Preferred pharmaceutically acceptable salts are those that are pharmacologically effective without undue toxicity, irritation, or allergic response, and are suitable for contact with the tissues of a subject. The compounds described herein may have sufficient acidic groups, sufficient basic groups, both types of functional groups, or more than one of each type, and thus react with a number of inorganic or organic bases and inorganic and organic acids to form pharmaceutically acceptable salts.

[0139] For compounds described herein that contain a basic group, such as an amine, pharma- ceutically acceptable salts can be prepared by any suitable method available in the art, for example, from inorganic acids, such as hydrochloric acid, hydrobromic acid, sulfuric acid, sulfamic acid, nitric acid, boric acid, phosphorous acid, and the like, or from organic acids, such as acetic acid, phenylacetic acid, propionic acid, stearic acid, lactic acid, ascorbic acid, maleic acid, hydroxymaleic acid, isethionic acid, succinic acid, valeric acid, fumaric acid, malonic acid, pyruvic acid, oxalic acid, glycolic acid, salicylic acid, oleic acid, palmitic acid, lauric acid, pyranosidyl acids, such as glucuronic acid or galacturonic acid, or from carboxylic acids, such as ... They may be prepared by treatment of the free base with an acid, an alpha-hydroxy acid such as mandelic acid, citric acid or tartaric acid, an amino acid such as aspartic acid or glutamic acid, an aromatic acid such as benzoic acid, 2-acetoxybenzoic acid, naphthoic acid or cinnamic acid, a sulfonic acid such as laurylsulfonic acid, p-toluenesulfonic acid, methanesulfonic acid or ethanesulfonic acid, or any compatible mixture of acids such as those given as examples herein, and any other acid and mixtures thereof which are regarded as equivalents or acceptable substitutes given the ordinary level of skill in the art.

[0140] For compounds described herein that contain an acidic group, such as a carboxylic acid group, base addition salts can be prepared by any suitable method available in the art, for example, by treating such compounds with a sufficient amount of the desired base, either neat or in a suitable inert solvent. Examples of pharma-ceutically acceptable base addition salts include, but are not limited to, lithium, sodium, potassium, calcium, ammonium, zinc or magnesium salts, or other metal salts; organic amino salts, such as alkyl, dialkyl, trialkyl or tetraalkyl ammonium salts.

[0141] Other examples of pharma- ceutically acceptable salts include, but are not limited to, camsylate, sulfate, pyrosulfate, bisulfate, sulfite, bisulfite, phosphate, monohydrogen phosphate, dihydrogen phosphate, metaphosphate, pyrophosphate, chloride, bromide, iodide, acetate, propionate, decanoate, caprylate, acrylate, formate, isobutyrate, caproate, heptanoate, propionate, oxalate, malonate, succinate, suberate, sebacate, fumarate, maleate, butyne-1,4-diol, tetrahydrofuran ... The salts include ethyl ester, hexyne-1,6-dioate, benzoate, chlorobenzoate, methylbenzoate, dinitrobenzoate, hydroxybenzoate, methoxybenzoate, phthalate, sulfonate, methylsulfonate, propylsulfonate, besylate, xylenesulfonate, naphthalene-1-sulfonate, naphthalene-2-sulfonate, phenylacetate, phenylpropionate, phenylbutyrate, citrate, lactate, γ-hydroxybutyrate, glycolate, tartrate and mandelate. A list of other suitable pharma- ceutically acceptable salts can be found in Remington's Pharmaceutical Sciences, 17th Edition, Mack Publishing Company, Easton, Pa., 1985.

[0142] The neutral forms of the compounds are preferably regenerated by contacting the salt with a base or acid and isolating the parent compound in the conventional manner. The parent form of the compound differs from the various salt forms in certain physical properties, such as solubility in polar solvents, but otherwise the salts are equivalent to the parent form of the compound for purposes of this application.

[0143] As used herein, the term "phenyl" refers to a -C 6 H 5 A phenyl group can be unsubstituted or substituted with one, two or three suitable substituents.

[0144] The terms "polycyclic", "polycyclic" and "polycyclic" refer to two or more rings (e.g., cycloalkyl, cycloalkenyl, cycloalkynyl, aryl, heteroaryl and / or heterocyclyl) in which two or more atoms are common to two adjacent rings, e.g., the rings are "fused rings". Each of the rings of a polycycle can be substituted or unsubstituted. In certain embodiments, each ring of a polycycle contains 3 to 10 atoms, preferably 5 to 7 atoms, in the ring.

[0145] As used herein, the term "prodrug" refers to a derivative of a known direct acting drug, which has enhanced delivery properties and therapeutic value compared to the drug, and is converted to an active drug by an enzymatic or chemical process. A common method for making a prodrug is to include one or more selected moieties that are hydrolyzed under physiological conditions to yield the desired molecule. In certain embodiments, the prodrug is converted by the enzymatic activity of the host animal. For example, a prodrug with a nitro group on the aromatic ring can be reduced in vivo by a reductase to yield the desired amino group of the corresponding active compound. In another example, functional groups such as hydroxyl, carbonate, or carboxylic acid in the parent compound are present as esters, which can be cleaved by esterases. In addition, amine groups in the parent compound are present in, but not limited to, carbamate, N-alkylated, or N-acylated forms (Simplicio et al, "Prodrugs for Amines," Molecules, (2008), 13:519-547). Certain embodiments In the formulations depicted above, some or all of the compounds described herein may be replaced with the corresponding suitable prodrugs.

[0146] As used herein, the term "purified" means that when isolated, the isolate contains at least 90%, at least 95%, at least 98%, or at least 99% of a compound described herein, by weight of the isolate.

[0147] As used herein, the phrase "quaternary ammonium salt" refers to a derivative of the disclosed compounds having one or more tertiary amine moieties, where at least one tertiary amine moiety in the parent compound is alkylated (and the cation is Cl). - , C.H. 3 COO - and CF 3 COO -such as methylation or ethylation), for example by converting the tertiary amine moiety to a quaternary ammonium cation via methylation or ethylation.

[0148] As used herein, the term "semicarbazone" refers to =NNHC(=O)NH 2 means...

[0149] As used herein, the phrase "solubilizing agent" means an agent that results in the formation of a micellar or true solution of a drug.

[0150] As used herein, the term "solution / suspension" refers to a liquid composition in which a first portion of an active agent is present in solution and a second portion of the active agent is present in a specific form in suspension in a liquid matrix.

[0151] As used herein, the phrase "substantially isolated" means a compound that is at least partially or substantially separated from the environment in which it is formed or detected.

[0152] The term "substituted" refers to a moiety having a substituent replacing a hydrogen on one or more carbons of the backbone. It will be understood that "substituted" or "substituted with" is implicit, provided that such substitution is in accordance with the permitted valencies of the substituted atom and substituent, and that the substitution results in a stable compound that does not spontaneously undergo transformation, for example, by rearrangement, cyclization, elimination, and the like. As used herein, the term "substituted" is intended to include all permissible substituents of organic compounds. In a broad aspect, the permissible substituents include acyclic and cyclic, branched and unbranched, carbocyclic and heterocyclic, aromatic and nonaromatic, substituents of organic compounds. The permissible substituents can be one or more than one and can be the same or different for appropriate organic compounds. For purposes of this application, heteroatoms such as nitrogen can have hydrogen substituents, and / or any permissible substituents of organic compounds described herein that satisfy the valencies of the heteroatoms.

[0153] The substituents may include any of the substituents described herein, such as halogen, hydroxyl, carbonyl (such as carboxyl, alkoxycarbonyl, formyl, or acyl), thiocarbonyl (such as thioester, thioacetate, or thioformate), alkoxyl, phosphoryl, phosphate, phosphonate, phosphinate, amino, amido, amidine, imine, cyano, nitro, azido, sulfhydryl, alkylthio, sulfate, sulfonate, sulfamoyl, sulfonamide, sulfonyl, heterocyclyl, aralkyl, or aromatic or heteroaromatic moieties. Those skilled in the art will understand that the substituents may themselves be substituted, where appropriate. Unless specifically stated as "unsubstituted," references to chemical moieties herein are understood to include substituted variants. For example, references to "aryl" groups or moieties implicitly include both substituted and unsubstituted variants.

[0154] The term "sulfate" is art-recognized and refers to the group -OSO 3H or a pharma- ceutically acceptable salt thereof.

[0155] The term "sulfonamide" is art-recognized and can be represented by the general formula [ka] (In the formula, R 29 and R 30 independently represent hydrogen or hydrocarbyl, e.g., alkyl, or R 29 and R 30 refers to a group represented by the formula: (which, together with the intervening atoms, completes a heterocycle having 4 to 8 atoms in the ring structure).

[0156] The term "sulfoxide" is art-recognized and refers to the group -S(O)-R 30 (In the formula, R 30 represents hydrocarbyl).

[0157] The term "sulfonate" is art-recognized and refers to the group SO 3 H or a pharma- ceutically acceptable salt thereof.

[0158] The term "sulfone" is art-recognized and refers to the group -S(O) 2 -R 30 (In the formula, R 30 represents hydrocarbyl).

[0159] As used herein, the phrase "therapeutically effective amount" refers to an amount of an active compound or pharmaceutical agent that induces a biological or pharmaceutical response desired in a tissue, system, animal, individual or human by a researcher, veterinarian, physician or other clinician. The therapeutic effect depends on the disorder being treated or the biological effect desired. Thus, the therapeutic effect may be a reduction in the severity of symptoms associated with the disorder and / or inhibition (partial or complete) of the progression of the disorder, or may improve the treatment, cure, prevention or elimination of the disorder or side effects. The amount required to induce a therapeutic response can be determined based on the age, health, size and sex of the subject. The optimal amount can also be determined based on monitoring the subject's response to the treatment.

[0160] The term "thioalkyl," as used herein, refers to an alkyl group substituted with a thiol group.

[0161] The term "thioester" as used herein refers to the group -C(O)SR 30 Or -SC(O)R 30 (In the formula, R 30 represents hydrocarbyl).

[0162] The term "thioether," as used herein, is an equivalent to an ether where the oxygen is replaced with a sulfur.

[0163] As used herein, the terms "treat", "treated" or "treating" refer to both therapeutic treatment and prophylactic measures, where the objective is to delay (reduce) an undesired physiological condition, disorder or disease, or to obtain a beneficial or desired clinical outcome. Beneficial or desired clinical outcomes include, but are not limited to, alleviation of symptoms; reduction in the extent of a condition, disorder or disease; stabilization (i.e., not worsening) of the condition, disorder or disease state; delaying the onset or slowing down the progression of the condition, disorder or disease; amelioration or alleviation (whether partial or total) of the condition, disorder or disease state, whether detectable or undetectable; restoration of at least one measurable physical parameter not necessarily recognized by the patient; or enhancement or amelioration of the condition, disorder or disease. Treatment includes eliciting a clinically significant response without excessive levels of side effects. Treatment also includes prolonging survival compared to expected survival in the absence of treatment. Thus, "treatment of erythropoietic protoporphyria" or "treating erythropoietic protoporphyria" refers to activities that reduce or ameliorate any of the primary or secondary symptoms associated with erythropoietic protoporphyria or other conditions described herein.

[0164] The term "urea" is art-recognized and has the general formula [ka] (In the formula, R 29 and R 30 independently represent hydrogen or hydrocarbyl, e.g., alkyl, or R 29 The appearance of R 30 and together with the intervening atoms complete a heterocycle having 4 to 8 atoms in the ring structure.

[0165] At various places in the present specification, substituents of compounds may be disclosed in groups or in ranges. It is specifically intended that embodiments include each and every individual subcombination of the members of such groups and ranges. For example, "C 1~6 The term "alkyl" includes methyl, ethyl, propyl, C 4 Alkyl, C 5 Alkyl and C 6 It is specifically intended to disclose alkyl individually.

[0166] For compounds in which a variable occurs more than once, each variable can be a different moiety selected from the Markush group defining the variable. For example, if a structure is described with two R groups co-occurring in the same compound, the two R groups can represent different moieties selected from the Markush group defined for R. In another example, multiple substituents can be optionally selected, e.g., [ka] When specified in the form: 1 is defined to include hydrogen, e.g., T 1 CH 2 , NH, etc., any H can be replaced with a substituent.

[0167] It will be further appreciated that certain features described herein, which are, for clarity, described in the context of separate embodiments, can also be provided in combination in a single embodiment. Conversely, various features that are, for brevity, described in the context of a single embodiment, can also be provided separately or in any suitable subcombination.

[0168] It is understood that the present embodiment encompasses the use of stereoisomers, diastereomers and optical stereoisomers of the compound, as well as mixtures thereof, if applicable.In addition, it is understood that stereoisomers, diastereomers and optical stereoisomers of the compound, as well as mixtures thereof, are within the scope of the embodiment.As a non-limiting example, the mixture may be racemic, or the mixture may contain one particular stereoisomer in an unequal proportion to the other.In addition, the compound may be provided as substantially pure stereoisomers, diastereomers, and optical stereoisomers (such as epimers).

[0169] The compounds described herein may be asymmetric (e.g., have one or more stereocenters). All stereoisomers, e.g., enantiomers and diastereomers, are intended to be included within the scope of the embodiments, unless otherwise indicated. Compounds containing asymmetrically substituted carbon atoms can be isolated in optically active or racemic forms. Methods for preparing optically active forms from optically active starting materials are known in the art, for example, by resolution of racemic mixtures or by stereoselective synthesis. Many geometric isomers of olefins, C=N double bonds, and the like, may also exist in the compounds described herein, and all such stable isomers are provided herein. Cis and trans geometric isomers of the compounds are also included within the embodiments, and can be isolated as a mixture of isomers or as separate isomeric forms. When a compound capable of stereoisomerism or geometric isomerism is specified by its structure or name without specifically referring to the R / S or cis / trans configuration, it is intended that all such isomers are contemplated.

[0170] In some embodiments, a composition comprises a compound, or a pharma- ceutically acceptable salt, solvate, or prodrug thereof, that is at least 90%, at least 95%, at least 98%, or at least 99%, or 100% enantiomerically pure, meaning that the ratio of one enantiomer to the other in the composition is at least 90:1, at least 95:1, at least 98:1, or at least 99:1, or is entirely in the form of one enantiomer relative to the other. In certain embodiments, a compound enriched in one enantiomer is substantially free of the other enantiomer, where substantially free means that the substance in question constitutes less than 10%, or less than 5%, or less than 4%, or less than 3%, or less than 2%, or less than 1%, for example, in a composition or mixture of compounds, relative to the amount of the other enantiomer. For example, if a composition or mixture of compounds contains 98 grams of a first enantiomer and 2 grams of a second enantiomer, it would be said to contain 98 mole percent of the first enantiomer and only 2% of the second enantiomer.

[0171] In certain embodiments, a compound enriched in one enantiomer is substantially free of the other enantiomer, where substantially free means that the substance in question constitutes less than 10%, or less than 5%, or less than 4%, or less than 3%, or less than 2%, or less than 1% relative to the amount of the other enantiomer in, for example, a composition or mixture of compounds. For example, if a composition or mixture of compounds contains 98 grams of a first enantiomer and 2 grams of a second enantiomer, it would be said to contain 98 mole percent of the first enantiomer and only 2% of the second enantiomer.

[0172] The resolution of racemic mixtures of compounds can be carried out by any of a number of methods known in the art, including, for example, chiral HPLC, fractional recrystallization using chiral resolving acids, which are organic acids that form optically active salts.Suitable resolving agents for fractional recrystallization include, but are not limited to, optically active acids, such as D and L forms of tartaric acid, diacetyltartaric acid, dibenzoyltartaric acid, mandelic acid, malic acid, lactic acid, and various optically active camphorsulfonic acids, such as β-camphorsulfonic acid.Other resolving agents suitable for fractional crystallization include, but are not limited to, stereoisomerically pure forms of α-methylbenzylamine (e.g., S and R forms, or diastereomerically pure forms), 2-phenylglycinol, norephedrine, ephedrine, N-methylephedrine, cyclohexylethylamine, 1,2-diaminocyclohexane, and the like. Resolution of racemic mixtures can also be carried out by elution on a column packed with an optically active resolving agent (e.g., dinitrobenzoylphenylglycine). The composition of a suitable elution solvent can be determined by one skilled in the art.

[0173] Compounds may also include tautomeric forms. Tautomeric forms result from the exchange of a single bond with an adjacent double bond together with the migration of a proton. Tautomeric forms include prototropic tautomers, which are isomeric protonation states with the same empirical formula and total charge. Examples of prototropic tautomers include, but are not limited to, ketone-enol pairs, amide-imidic acid pairs, lactam-lactim pairs, amide-imidic acid pairs, enamine-imine pairs, and cyclic forms when protons can occupy two or more positions of a heterocyclic system, including, but not limited to, 1H- and 3H-imidazole, 1H-, 2H- and 4H-1,2,4-triazole, 1H- and 2H-isoindole, and 1H- and 2H-pyrazole. Tautomeric forms may be in equilibrium or sterically fixed into one form by appropriate substitution.

[0174] Glycine transporter inhibitors, such as GlyT1 inhibitors, including their pharma- ceutically acceptable salts (e.g., GlyT1 inhibitors disclosed herein), can also exist as hydrates and solvates, as well as anhydrous and nonsolvated forms. A "hydrate" is a compound that exists as a composition with water molecules. The composition can contain a stoichiometric amount of water, e.g., a monohydrate or dihydrate, or can contain a random amount of water. A "solvate" is a similar composition, except that a solvent other than water, e.g., methanol, ethanol, dimethylformamide, diethyl ether, etc., is substituted for water. For example, methanol or ethanol can form an "alcoholate," which again can be stoichiometric or nonstoichiometric. Mixtures of such solvates or hydrates can also be prepared. The origin of such solvates or hydrates can be derived from the solvent of crystallization, inherent in the solvent of preparation or crystallization, or associated with such solvent.

[0175] The compounds of the present application, including their pharma- ceutically acceptable salts and prodrugs, may exist as various polymorphs, pseudopolymorphs, or amorphous states. The term "polymorphs" as used herein refers to different crystalline forms of the same compound, and other solid state molecular forms, including pseudopolymorphs such as hydrates, solvates, or salts of the same compound. Different crystalline polymorphs have different crystal structures due to different packing of molecules in the lattice as a result of changes in temperature, pressure, or variables in the crystallization process. Polymorphs differ from each other in their physical properties, such as X-ray diffraction properties, stability, melting point, solubility, or rate of dissociation in a certain solvent. Therefore, the form of crystalline polymorphs is an important aspect in the development of suitable dosage forms in the pharmaceutical industry.

[0176] Compounds may also include atoms of all isotopes occurring in intermediates or final compounds. Isotopes include atoms having the same atomic number but different mass numbers. For example, isotopes of hydrogen include tritium and deuterium.

[0177] In some embodiments, the compound or its salt is substantially isolated. Partial separation may include, for example, a composition enriched with the compound. Substantial separation may include a composition containing at least about 50% by weight, at least about 60% by weight, at least about 70% by weight, at least about 80% by weight, at least about 90% by weight, at least about 95% by weight, at least about 97% by weight, or at least about 99% by weight of the compound or its salt. Methods for isolating compounds and their salts are routine in the art.

[0178] While the disclosed compounds are preferred, other functional groups can be introduced into the compounds with the expectation of similar results. In particular, thioamides and thioesters are expected to have very similar properties. The distance between the aromatic rings can affect the geometric pattern of the compounds, and can be substituted as necessary, or this distance can be modified by introducing aliphatic chains of different lengths that can include amino acids, dicarboxylic acids, or diamines. The distance between the monomers in the compounds and their relative orientation can also be modified by replacing the amide bond with a substitute that has an additional atom. Thus, the replacement of the carbonyl group with a dicarbonyl changes the distance between the monomers and the tendency of the dicarbonyl unit to adopt the anti configuration of the two carbonyl moieties, changing the periodicity of the compounds. Pyromellitic anhydride represents yet another alternative to the simple amide linkage, which can change the conformation and physical properties of the compounds. Recent methods of solid phase organic chemistry (E. Atherton and RC Sheppard, Solid Phase Peptide Synthesis A Practical The present approach (IRL Press Oxford 1989) now allows the synthesis of homodisperse compounds with molecular weights approaching 5,000 daltons. Other substitution patterns are similarly effective.

[0179] The compounds also include derivatives called prodrugs.

[0180] Compounds containing amine functional groups can also form N-oxides. Reference herein to compounds containing amine functional groups also includes N-oxides. When a compound contains several amine functional groups, one or more nitrogen atoms can be oxidized to form N-oxides. Examples of N-oxides include the N-oxides of tertiary amines or nitrogen atoms of nitrogen-containing heterocycles. N-oxides can be formed by treating the corresponding amine with an oxidizing agent such as hydrogen peroxide or peracid (e.g., peroxycarboxylic acid) (see Advanced Organic Chemistry, by Jerry March, 4th Edition, Wiley Interscience).

[0181] This may be claimed according to ranges or in any similar manner, with the right to proviso or exclude any individual member of any such group, including any subrange or combination of subranges within the group, and less than the full scale of the disclosure may be claimed for any reason. Furthermore, this may be claimed according to ranges or in any similar manner, with the right to proviso or exclude any individual substituent, analog, compound, ligand, structure or group of the claimed group, or any member thereof, and less than the full scale of the disclosure may be claimed for any reason. Throughout this disclosure, various patents, patent applications and publications are referenced. The disclosures of these patents, patent applications and publications are incorporated by reference in their entirety into this disclosure in order to more fully describe the state of the art as of the date of this disclosure to those skilled in the art. In the event of any inconsistency between the cited patents, patent applications and publications and this disclosure, the present disclosure will control.

[0182] For convenience, certain terms used in the specification, examples, and claims are collected here. Unless otherwise defined, all technical and scientific terms used in this disclosure have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs.

[0183] Various embodiments of the compounds and salts thereof are provided. If a variable is not specifically recited, the variable may be any of the options described herein unless otherwise stated or indicated by context.

[0184] In some embodiments, the compound is as set forth in the accompanying non-limiting exemplary claims, or a pharma- ceutically acceptable salt, solvate, or prodrug thereof.

[0185] In some embodiments of the methods and uses disclosed herein, the GlyT1 inhibitor is [ka] [In the formula, Ar is an unsubstituted or substituted aryl or 6-membered heteroaryl containing 1, 2 or 3 nitrogen atoms, where the substituted aryl and heteroaryl groups are hydroxy, halogen, NO 2 ,CN,(C 1 ~C 6 )-alkyl, halogen-substituted (C 1 ~C 6 )-alkyl, hydroxy substituted (C 1 ~C 6 )-alkyl, (CH 2 )n-(C 1 ~C 6 )-alkoxy, halogen-substituted (C 1 ~C 6 )-Alkoxy, NR 7 R 8 , C(O)R 9 , SO2R 10 and -C(CH 3 )=NOR 7or (C 1 ~C 6 )-substituted by a 5-membered aromatic heterocycle containing 1 to 4 heteroatoms selected from N and O, optionally substituted by alkyl; R 1 is hydrogen or (C 1 ~C 6 )-alkyl; R 2 is hydrogen, (C 1 ~C 6 )-alkyl, (C 2 ~C 6 )-alkenyl, halogen-substituted (C 1 ~C 6 )-alkyl, hydroxy substituted (C 1 ~C 6 )-alkyl, (C 1 ~C 6 )-alkoxy or halogen optionally substituted (CH2)n-(C 3 ~C 7 )-cycloalkyl, CH(CH 3 )-(C 3 ~C 7 )-cycloalkyl, (CH 2 ) n+1 -C(O)-R 9 , (CH 2 ) n+1 -CN, bicyclo[2.2.1]heptyl, (CH 2 ) n+1 -O-(C 1 ~C 6 )-alkyl, (CH 2 ) n -heterocycloalkyl, (CH 2 ) n -aryl or (CH 2 ) n - 5- or 6-membered heteroaryl containing 1, 2 or 3 heteroatoms selected from the group consisting of oxygen, sulfur or nitrogen (CH 2 ) n- 5- or 6-membered heteroaryl, where aryl, heterocycloalkyl and heteroaryl are unsubstituted or substituted with hydroxy, halogen, (C 1 ~C 6 )-Alkyl and (C 1 ~C 6 )-alkoxy; R 3 , R 4 and R 6 are each independently hydrogen, hydroxy, halogen, (C 1 ~C 6 )-alkyl, (C 1 ~C 6 )-alkoxy or O-(C 3 ~C 6 )-cycloalkyl; R 5 No 2 , C.N., C(O)R. 9 or SO 2 R 10 and; R 7 and R 8 are each independently hydrogen or (C1-C6)-alkyl; R 9 is hydrogen, (C 1 ~C 6 )-alkyl, (C 1 ~C 6 )-alkoxy or NR 7 R 8 and; R 10 is optionally substituted with halogen (C 1 ~C 6 )-alkyl, (CH 2 ) n -(C 3 ~C 6 )-cycloalkyl, (CH 2 ) n -(C 3 ~C 6 )-alkoxy, (CH 2 ) n -heterocycloalkyl or NR 7 R8 and; n is 0, 1 or 2. or a pharma- ceutically acceptable salt thereof, or a prodrug of the compound or a pharma- ceutically acceptable salt thereof.

[0186] In some embodiments of the methods and uses disclosed herein, the GlyT1 inhibitor is [ka] or a pharma- ceutically acceptable salt thereof, or a prodrug of the compound or a pharma- ceutically acceptable salt thereof.

[0187] In some embodiments of the methods and uses disclosed herein, the GlyT1 inhibitor is [ka] [In the formula, R 1 represents a heteroaryl selected from the group consisting of imidazolyl, thiazolyl, pyridyl, oxazolyl, pyrazolyl, triazolyl, oxadiazolyl, quinolinyl, isoxazolyl, pyrrolyl, and thiadiazole, wherein said heteroaryl is selected from the group consisting of -OH, -NR 7 R 8 , halogens, (C 1 ~C 8 ) alkyl, (C 3 ~C 10 ) cycloalkyl, (C 1 ~C 8 ) alkoxy, (C 1 ~C 12 ) alkoxyalkyl, (C 1 ~C 8 ) hydroxyalkyl, (C 6 ~C 14 ) optionally substituted with one or more substituents selected from aryl and benzyl; R 2 , R 3 and A are independently H or (C1 ~C 8 )alkoxy, where the alkyl is selected from one or more -OH, (C 1 ~C 8 ) Alkoxy, -NR 7 R 8 or optionally substituted by halogen; Q is -(CH 2 ) n -(wherein n=1, 2, 3 or 4) or -(CH 2 ) m -O- (wherein m=2, 3 or 4); Z is (C 6 ~C 14 ) aryl, (C 1 ~C 8 ) alkyl or (C 3 ~C 8 ) represents cycloalkyl; R 4 and R 5 are each independently H, halogen, (C 1 ~C 8 ) alkyl, (C 6 ~C 14 ) aryl, (C 6 ~C 14 )aryloxy, (C 1 ~C 8 )alkoxy, (3-10 membered)heterocycloalkyl or (C 3 ~C 8 ) cycloalkoxy; where R 4 and R 5 is one or more -OH, (C 1 ~C 8 ) Alkoxy, -NR 7 R 8 or optionally substituted by halogen; Y is -R 6 , -(CH 2 )oR 6 , -C(R 6 ) 3 or -CH(R 6 ) 2 where 0=1, 2 or 3; R6 is H, (C 6 ~C 14 ) aryl, (C 1~10 ) alkyl, (C 3 ~C 10 ) cycloalkyl, (C 5 ~C 18 ) bicycloalkyl, (C 5 ~C 18 )tricycloalkyl, (3-10 membered)heterocycloalkyl, (5-10 membered)heteroaryl, -C(=O)NR 7 R 8 OR -C(=O)OR 7 (Wherein, R 6 The group may be optionally substituted with one or more X groups; X=-OH, (C 1 ~C 8 ) Alkoxy, -NR 11 R 12 , -SO 2 R 10 , -C(=O)R 10 , halogen, cyano, (C 1 ~C 8 ) alkyl, (C 1 ~C 10 )alkoxyalkyl, (5-10 membered)heteroaryl, (C 6 ~C 14 ) aryl, (C 6 ~C 14 )aryloxy, benzyl or (C1-C 8 ) hydroxyalkyl; R 7 and R 8 are independently H, (C 1 ~C 8 ) alkyl, (C 3 ~C8) cycloalkyl, (5-10 membered) heterocycloalkyl, (C 1 ~C 8 ) hydroxyalkyl, (5-10 membered) heteroaryl or (C 1 ~C 10 ) alkoxyalkyl; R 7 and R 8is optionally substituted with one or more X groups; Or R 7 and R 8 may, together with the nitrogen to which they may be attached, form a (3-10 membered) heterocycloalkyl group optionally substituted with one or more X groups; R 10 is (C 1 ~C 8 ) alkyl, (C 3 ~C 8 )cycloalkyl, (3-10 membered)heterocycloalkyl, (C 1 ~C 8 ) hydroxyalkyl, (5-10 membered) heteroaryl or (C 1 ~C 10 ) alkoxyalkyl; R 11 and R 12 are independently H, (C 1 ~C 8 ) alkyl, (C 3 ~C 8 )cycloalkyl, (5-10 membered)heterocycloalkyl, (C 1 ~C 8 ) hydroxyalkyl, (5-10 membered) heteroaryl or (C 1 ~C 10 ) represents alkoxyalkyl] or a pharma- ceutically acceptable salt thereof, or a prodrug of the compound or a pharma- ceutically acceptable salt thereof.

[0188] In some embodiments of the methods and uses disclosed herein, the GlyT1 inhibitor is [ka] or a pharma- ceutically acceptable salt thereof, or a prodrug of the compound or a pharma- ceutically acceptable salt thereof.

[0189] In some embodiments of the methods and uses disclosed herein, the GlyT1 inhibitor is [ka] or a pharma- ceutically acceptable salt thereof, or a prodrug of the compound or a pharma- ceutically acceptable salt thereof.

[0190] In some embodiments of the methods and uses disclosed herein, the GlyT1 inhibitor is [ka] [In the formula, Z 1 is C 1~4 Alkyl, C 3~6 Cycloalkyl, C 1~4 Alkoxy, C 1~4 Alkylthio, HaloC 1~4 Alkyl, phenyl, haloC 1~4 Alkoxy, halophenyl, C 1~4 Alkyl sulfoxy, C 1~4 Selected from the group consisting of alkylsulfonyl, bromo and chloro; Z 2 is hydrogen, halogen, cyano, C 1~4 Alkyl, phenyl, haloC 1~4 Alkyl, haloC 1~4 Alkoxy, halophenyl, C 1~4 Alkoxy C 1~4 Alkyl and C 3~6 cycloalkyl; Z 3 is hydrogen, halogen, C 1~4 Alkyl, C 1~4 Alkoxy, C 1~4 Alkylthio, HaloC 1~4 Alkyl, haloC 1~4 Alkoxy and C 3~6 cycloalkyl; Z 4 is hydrogen, halogen, C1-3 alkyl, haloC 1~4 Alkyl, C 1~4 Alkoxy, C 1~4 Alkylthio, Phenyl, HaloC 1~4Alkoxy, halophenyl, C 1~4 Alkoxy C 1~4 Alkyl and C 3~6 cycloalkyl; Z 5 is hydrogen, fluoro, chloro, bromo, iodo, hydroxy, C 1~4 Alkyl, C 1~4 Alkoxy, C 1~4 Alkylthio, Phenyl, HaloC 1~4 Alkyl, haloC 1~4 Alkoxy, halophenyl, C 1~4 Alkoxy C 1~4 Alkyl and C 3~6 cycloalkyl; Here, Z 1 ~Z 5 When more than one of Z is methoxy, 1 and Z 5 Only R is methoxy. 3 and R 4 are independently selected from hydrogen and C optionally substituted with one or more groups Y. 1~4 alkyl; or R 3 and R4 together with the nitrogen atom to which they are attached form a saturated or partially unsaturated A, 5-, 6- or 7-membered carbocyclic ring optionally substituted with a group Y'; Y is C 1~4 Alkoxy, Hydroxy, HaloC 1~4 Alkoxy and C 3~5 cycloalkyl; Y' is C 1~4 Alkyl, C 1~4 Alkoxy, halogen, hydroxy, haloC 1~4 Alkoxy, C 3~5 Cycloalkyl and C 5~10 aryl, or Y' forms a -CH2- or -CH2-CH2- bridge between two atoms on A, a 5-, 6- or 7-membered carbocyclic ring; R 5 and R 6 is independently a C optionally substituted with one or more groups X; 1~4alkyl; or R 5 and R 6 together with the carbon atoms to which they are attached form a saturated 5- or 6-membered carbocyclic ring optionally substituted with one or more groups X'; R 5 and when R6 together with the carbon atom to which they are attached form a 5-membered saturated carbocyclic ring, the ring may optionally further contain a group of additional heteroatoms selected from O, N and S(O)m, where m=0, 1 or 2; X is halogen, hydroxy, C 1~4 Alkoxy, HaloC 1~4 Alkyl, haloC 1~4 Alkoxy and C 5~10 aryl; X' is halogen, hydroxy, C 1~4 Alkyl, C 1~4 Alkoxy, HaloC 1~4 Alkyl, haloC 1~4 Alkoxy and C 5~10 aryl; where R 3 , R 4 , R 5 and R 6 are not all simultaneously unsubstituted methyl; However, at the same time, Z 1 is propyloxy, and Z 3 is chloro and Z 2 =Z 4 =Z 5 =H and R 5 and R 6 If both are methyl, R 3 and R 4 do not form a 2-methylpyrrolidine group together with the nitrogen atom to which they are attached; 1 is methyl and Z 3 is methoxy and Z 2 = Z4 = Z5 = H, and R 5 and R 6 If both are methyl, R 3 and R 4do not form, together with the nitrogen atom to which they are attached, a pyrrolidine group. or a pharma- ceutically acceptable salt thereof, or a prodrug of the compound or a pharma- ceutically acceptable salt thereof.

[0191] In some embodiments of the methods and uses disclosed herein, the GlyT1 inhibitor is [ka] or a pharma- ceutically acceptable salt thereof, or a prodrug of the compound or a pharma- ceutically acceptable salt thereof.

[0192] In some embodiments of the methods and uses disclosed herein, the GlyT1 inhibitor is [ka] [In the formula, Z is (CH 2 ) n , O, S, SO, SO 2 or NR 5 and; n is 0, 1 or 2; X is hydrogen, halogen, (C 1~6 ) alkyloxy, (C 3~6 ) cycloalkyloxy, (C 6~12 )aryloxy, (C 6~12 )Aryl, Thienyl, SR6, SOR6, SO 2 R 6 , N.R. 6 R 6 , N.H.R. 6 , N.H. 2 , N.H.C.O.R. 6 , NSO 2 R 6 , CN, COOR 6 , and halogens, (C 6~12 ) aryl, (C 1~6 ) alkyloxy or (C 6~12 ) optionally substituted with aryloxy (C 1~4) alkyl; or two substituents at adjacent positions together represent a fused (C 5~6 ) aryl group, fused (C 5~6 ) cycloalkyl ring or O-(CH 2 ) m -O; m is 1 or 2; Y is hydrogen, halogen, (C 1~4 ) Alkyloxy, SR 6 , N.R. 6 R 6 , and optionally substituted with halogen (C 1~4 ) represents 1 to 3 substituents independently selected from alkyl; R 1 COOR 7 or CONR 8 R 9 and; R 2 and R6 is (C 1~4 ) alkyl; R 3 , R 4 and R 5 are independently hydrogen or (C 1~4 ) alkyl; R 7 , R 8 and R 9 are independently hydrogen, (C 1~4 ) alkyl, (C 6~12 ) aryl or arylalkyl] or a pharma- ceutically acceptable salt thereof, or a prodrug of the compound or a pharma- ceutically acceptable salt thereof.

[0193] In some embodiments of the methods and uses disclosed herein, the GlyT1 inhibitor is [ka] or a pharma- ceutically acceptable salt thereof, or a prodrug of the compound or a pharma- ceutically acceptable salt thereof.

[0194] In some embodiments of the methods and uses disclosed herein, the GlyT1 inhibitor is [ka] [In the formula, n is an integer from 1 to 3; R 1 and R 2 is independently selected from hydrogen, alkyl, haloalkyl, alkoxy, haloalkoxy, aryl, heteroaryl, cycloalkyl, or heterocyclyl, wherein said rings are independently selected from alkyl, halo, haloalkyl, alkoxy, haloalkoxy, hydroxy, cyano, monosubstituted amino, or disubstituted amino; a , R b or R c with R 1 and R 2 when attached to the same carbon atom, can be combined to form a cycloalkyl or a monocyclic saturated heterocyclyl to give a spirocycle, where the cycloalkyl or monocyclic saturated heterocyclyl is independently selected from alkyl, alkoxy, fluoro, fluoroalkyl, fluoroalkoxy, hydroxy, monosubstituted amino, or disubstituted amino. d , R c or R f or R 1 and R 2 When attached to the 2- and 5- or 3- and 6-carbon atoms of a piperazine ring, they combine to form -C 1 ~C 3 - an alkylene chain can be formed, in which one of the carbon atoms in the alkylene chain is optionally replaced by -NR-, -O-, -S(O)n- (wherein R is hydrogen or alkyl and n is 0-2), and further, one or two hydrogen atoms in the alkylene chain can be optionally replaced by one or two alkyl; R 3 , R 4 and R 5are independently hydrogen, alkyl, fluoro or fluoroalkyl; Ar 1 and Ar 2 is independently aryl, heteroaryl, cycloalkyl, or heterocyclyl, where each of the foregoing rings is selected from R g , R h or R i and R g is alkyl, -C=CR 6 (In the formula, R 6 is aryl or heteroaryl), halo, haloalkyl, haloalkoxy, alkylthio, cyano, alkoxy, amino, monosubstituted amino, disubstituted amino, sulfonyl, acyl, carboxy, alkoxycarbonyl, hydroxyalkyl, alkoxyalkyl, aminoalkyl, hydroxyalkoxy, alkoxyalkoxy, aminoalkoxy, aminosulfonyl, aminocarbonyl, or acylamino; R h and R i is independently selected from alkyl, halo, haloalkyl, haloalkoxy, alkylthio, cyano, alkoxy, amino, monosubstituted amino, disubstituted amino, sulfonyl, acyl, carboxy, alkoxycarbonyl, hydroxyalkyl, alkoxyalkyl, aminoalkyl, hydroxyalkoxy, alkoxyalkoxy, aminoalkoxy, aminosulfonyl, aminocarbonyl, acylamino, aryl, heteroaryl, cycloalkyl, or heterocyclyl, where R g , R h and R i R is independently selected from alkyl, halo, haloalkyl, haloalkoxy, alkylthio, cyano, alkoxy, amino, monosubstituted amino, disubstituted amino, sulfonyl, acyl, carboxy, alkoxycarbonyl, hydroxyalkyl, alkoxyalkyl, aminoalkyl, hydroxyalkoxy, alkoxyalkoxy, aminoalkoxy, aminosulfonyl, aminocarbonyl, or acylamino. j , R k or R lwith the proviso that the compound of formula V is not 2-(4-benzhydrylpiperazin-l-yl)acetic acid, 2-(4-((4-chlorophenyl)(phenyl)methyl)piperazin-l-yl)acetic acid, 2-((2R,5S)-4-((R)-(4-(lH-tetrazol-5-yl)phenyl)(3-hydroxyphenyl)methyl)-2,5-dimethylpiperazin-l-yl)acetic acid or 2-((2R,5S)-4-((R)-(4-cyanophenyl)(3-hydroxyphenyl)methyl)-2,5-dimethylpiperazin-l-yl)acetic acid. or a pharma- ceutically acceptable salt thereof, or a prodrug of the compound or a pharma- ceutically acceptable salt thereof.

[0195] In some embodiments of the methods and uses disclosed herein, the GlyT1 inhibitor is [ka] or a pharma- ceutically acceptable salt thereof, or a prodrug of the compound or a pharma- ceutically acceptable salt thereof.

[0196] In some embodiments of the methods and uses disclosed herein, the GlyT1 inhibitor is [ka] [In the formula, A is a group of the general formula NR 1 Groups of the general formula N+(O−)R 1 or a group of the general formula N+(R')R 1 (wherein R 1 is a straight-chain or branched (C 1 ~C 7 ) alkyl group, or (C 4 ~C 7 ) a cycloalkyl group, or (C 3 ~C 7 )Cycloalkyl(C 1 ~C 3) alkyl group, or phenyl optionally substituted with one or two hydroxyl or methoxy groups (C 1 ~C 3 ) alkyl group, or (C 2 ~C 4 ) an alkenyl group, or (C 2 ~C 4 ) an alkynyl group; R' is a linear or branched (C 1 ~C 7 ) representing an alkyl group; X is a hydrogen atom, or a halogen atom and trifluoromethyl, linear or branched (C1-C4) alkyl and (C 1 ~C 4 ) alkoxy groups; R 2 is a hydrogen atom, or a halogen atom and trifluoromethyl, (C 1 ~C 4 ) alkyl group or (C 1 ~C 4 ) an alkoxy group, or a group of the general formula NR 3 R 4 (wherein R 3 and R 4 are each independently a hydrogen atom or (C 1 ~C 4 ) an alkyl group or, together with the nitrogen atom which they bear, form a pyrrolidine, piperidine or morpholine ring, or a phenyl group optionally substituted with atoms or groups as defined above for the symbol X), or a pharma- ceutically acceptable salt thereof, or a prodrug of the compound or a pharma- ceutically acceptable salt thereof.

[0197] In some embodiments of the methods and uses disclosed herein, the GlyT1 inhibitor is [ka] or a pharma- ceutically acceptable salt thereof, or a prodrug of the compound or a pharma- ceutically acceptable salt thereof.

[0198] In some embodiments of the methods and uses disclosed herein, the GlyT1 inhibitor has the formula [ka] [In the formula, R 1 is -(CH 2 ) n -R 1a (In the formula, n is independently 0 to 6; R 1a teeth, (1) C unsubstituted or substituted with 1 to 6 halogen or hydroxyl 1~6 Alkyl, (2)R 2a , R 2b and R 2c phenyl substituted with (3) Unsubstituted or C 1~6 Alkyl, 1-6 halogens, hydroxy or -NR 10 R 11 C replaced with 3~6 Cycloaryl, (4) unsubstituted or substituted with 1 to 6 halogen, hydroxy or -NR 10 R 11 -OC is substituted with 1~6 Alkyl, (5)-CO2R 9 (Wherein, R9 is (a) hydrogen, (b) -C which is unsubstituted or substituted with 1 to 6 fluoro. 1~6 Alkyl, (c) benzyl, and (d) Phenyl ), (6)-NR 10 R 11 (In the formula, R 10 and R11 teeth, (a) hydrogen, (b) is unsubstituted or is hydroxy, 1 to 6 fluoro, or -NR 12 R 13 (In the formula, R 12 and R 13 is hydrogen and -C 1~6 substituted with -C 1~6 Alkyl, (c) is unsubstituted or is hydroxy, 1 to 6 fluoro, or -NR 12 R 13 -C is substituted with 3~6 Cycloalkyl, (d) benzyl, (e) Phenyl (independently selected from (7)-CONR 10 R 11 is selected from the group consisting of R 2 teeth, (1)R 2a , R 2b and R 2c phenyl substituted with (2) unsubstituted or substituted with 1 to 6 halogen, hydroxy, -NR 10 R 11 , C substituted with phenyl or heterocycle 1~8 Alkyl (wherein phenyl or heterocycle is R 2a , R 2b and R 2c ), (3) unsubstituted or substituted with 1 to 6 halogen, hydroxy or -NR 10 R 11 C replaced with 3~6 cycloalkyl, and (4) unsubstituted or substituted with 1 to 6 halogen, hydroxy or -NR 10 R 11 -C is substituted with 1~6 Alkyl-(C 3~6 Cycloalkyl) selected from the group consisting of; R 2a , R 2b and R 2c teeth, (1) Hydrogen, (2) halogens, (3) unsubstituted, or (a) 1 to 6 halogens, (b) phenyl, (c)C 3~6 cycloalkyl, or (d)-NR 10 R 11 -C is substituted with 1~6 Alkyl, (4) -OC, which is unsubstituted or substituted with 1 to 6 halogens 1~6 Alkyl, (5) Hydroxy, (6)-SCF 3 , (7)-SCHF 2 , (8)-SCH 3 , (9)-CO 2 R 9 , (10)-CN, (11)-SO 2 R 9 , (12)-SO 2 -NR 10 R 11 , (13)-NR 10 R 11 , (14)-CONR 10 R 11 , and (15)-NO 2 independently selected from the group consisting of: R 3 teeth, (1) unsubstituted or substituted with 1 to 6 halogen, hydroxyl or -NR 10 R 11 C replaced with 1~6 Alkyl, (2) unsubstituted or substituted with 1 to 6 halogen, hydroxyl or -NR 10 R 11 C replaced with 3~6 Cycloalkyl is selected from the group consisting of R 4 and R 5 teeth, (1) Hydrogen, and (2) C which is unsubstituted or substituted with halogen or hydroxyl 1~6 Alkyl or R 4 and R 5 Together, C 3~6 forming a cycloalkyl ring; A is, (1)-O-, and (2)-NR 10 - selected from the group consisting of; m is zero or one, where, when m is zero, R 2 directly bonds to the carbonyl. and pharma- ceutically acceptable salts thereof, and the individual enantiomers and diastereomers thereof or pharma- ceutically acceptable salts thereof, or a prodrug of the compound or a pharma- ceutically acceptable salt thereof.

[0199] In some embodiments of the methods and uses disclosed herein, the GlyT1 inhibitor is [ka] or a pharma- ceutically acceptable salt thereof, or a prodrug of the compound or a pharma- ceutically acceptable salt thereof.

[0200] In some embodiments of the methods and uses disclosed herein, the GlyT1 inhibitor is [ka] [In the formula, R 1 is a halogen, C 1 ~C 3 Alkyl, C 3 ~C 6 Cycloalkyl, OR 9 or S.R. 10 phenyl independently substituted 1 to 5 times with, 1 ~C 3 Alkyl and C 3 ~C 6 Cycloalkyl is R 7 is substituted 1 to 10 times as necessary; R 2 is H; R 3 and R4 are each independently H or CH 3 and; R 5 teeth, (1) Hydrogen, (2)R 7 C optionally substituted 1 to 11 times with 1 ~C 6 Alkyl, (3) gem-dialkyl, and (4) gem-dihalogen or Two R on the same carbon 5 The substituents, together with the carbon atom to which they are attached, form R 7 or forming a 3-, 4- or 5-membered cycloalkyl optionally substituted 1 to 10 times with Two R on adjacent carbons of the ring to which they are attached 5 The substituents, taken together, are R 7 may form a 3-, 4-, 5- or 6-membered cycloalkyl optionally substituted 1 to 10 times with R 6 teeth, [ka] (wherein E, F, and G are each independently nitrogen or carbon; R 6a is C optionally substituted 1 to 5 times with halogen or deuterium 1 ~C2 alkyl); R 7 teeth, (1) Hydrogen, (2) halogens, (3) Deuterium, (4) gem-dialkyl, (5) gem-dihalogen, (6)-OR 9 , -NR 11 R 12 , -NR 11 C(O) p R 10 , -S(O) p R 10 , -CN, -NO2, -C(O) p R 10 , -C(O)NR 11 R 12 or NR 11 C(S)R 10 , and (7) oxo or thio selected from the group consisting of; R 8 teeth, (1) Hydrogen, (2) halogens, (3) C 1 ~C 6 Alkyl, C 2 ~C 6 Alkenyl, C 2 ~C 6 Alkynyl, C 3 ~C 7 Cycloalkyl or C 4 ~C 7 Cycloalkylalkyl (Here, C 1 ~C 6 Alkyl, C 2 ~C 6 Alkenyl, C 2 ~C 6 Alkynyl, C 3 ~C 7 Cycloalkyl and C 4 ~C 7 Each cycloalkylalkyl is independently R 7 substituted 1 to 11 times as necessary), or (4)-OR 9 , -NR 11 R 12 , -NR 11 C(O) p R 10 , -S(O) p R 10 , -CN, -NO2, -C(O) p R 10 , -C(O)NR 11 R 12 Or -NR 11 C(S)R 10 selected from the group consisting of; R 9 is hydrogen, C 1 ~C 4 Alkyl, C 3 ~C 7 Cycloalkyl, C 4 ~C 7 Cycloalkylalkyl, -C(O)NR 11 R 12 and -C(O) p R 10 wherein C is selected from the group consisting of 1 ~C 4 Alkyl, C 3 ~C 7 Cycloalkyl and C 4 ~C 7 Each of the cycloalkylalkyls is R 7 with 1 to 11 optional substitutions; R 10 is hydrogen, C 1 ~C 4 Alkyl, C 3 ~C 7 Cycloalkyl, C 4 ~C 7 is selected from the group consisting of cycloalkyl, alkyl, aryl and heteroaryl, 1 ~C 4 Alkyl, C 3 ~C 7 Cycloalkyl and C 4 ~C 7Each cycloalkylalkyl is optionally substituted 1 to 11 times with a substituent defined in R7, and an aryl or heteroaryl is optionally substituted 1 to 11 times with a substituent defined in R 8 is substituted 1 to 10 times as necessary; R 11 and R 12 is hydrogen, C 1 ~C 4 Alkyl, C 3 ~C 7 Cycloalkyl, C 4 ~C 7 each independently selected from the group consisting of cycloalkyl, alkyl, aryl, and heteroaryl, 1 ~C 4 Alkyl, C 3 ~C 7 Cycloalkyl and C 4 ~C 7 Each of the cycloalkylalkyls is R 7 and the aryl or heteroaryl is optionally substituted 1 to 11 times with a substituent as defined in 8 or R 11 and R 12 together with the nitrogen to which they are attached, R 7 forming a saturated or partially saturated monocyclic or fused bicyclic heterocycle optionally substituted 1 to 11 times with; A is, [ka] and; X is N; Y is N; p is 1 or 2; m is 0; However, R 6 cannot be (a) 1H-1,2,3-triazol-4-yl, or (b) 5-methylisoxazol-4-yl. or an oxide thereof, a pharma- ceutically acceptable salt of the compound or the oxide thereof, or an individual enantiomer or diastereomer thereof.

[0201] In some embodiments of the methods and uses disclosed herein, the GlyT1 inhibitor is [ka] [ka] [ka] [ka] or a pharma- ceutically acceptable salt thereof, or a prodrug of the compound or a pharma- ceutically acceptable salt thereof.

[0202] In some embodiments of the methods and uses disclosed herein, the GlyT1 inhibitor is [ka] or a pharma- ceutically acceptable salt thereof, or a prodrug of the compound or a pharma- ceutically acceptable salt thereof.

[0203] In some embodiments of the methods and uses disclosed herein, the GlyT1 inhibitor is [ka] [In the formula, R 1 represents phenyl or a 5- or 6-membered monocyclic heteroaryl having 1, 2 or 3 heteroatoms independently selected from O, N or S, where the phenyl or heteroaryl is optionally joined to one or more R 3 with substitutions as necessary; R 2represents an aryl, a 5- or 6-membered monocyclic heteroaryl, or an 8-10-membered bicyclic heteroaryl, where the monocyclic or bicyclic heteroaryl has 1, 2, or 3 heteroatoms independently selected from O, N, or S, and where the aryl or heteroaryl has one or more R 4 with substitutions as necessary; R 3 is a halogen, C 1~4 -Alkyl or C 3~6 -cycloalkyl, where C 1~4 -Alkyl or C 3~6 -cycloalkyl is optionally substituted with one or more halogens; R 4 is halogen, -CN, C 1~4 -Alkyl, C 3~6 -cycloalkyl, -C 1~3 -Alkyl-C 3~6 -Cycloalkyl or -OC 1~6 alkyl, where C 1~4 -Alkyl, C 3~6 -cycloalkyl, -C 1~3 -Alkyl-C 3~6 -Cycloalkyl or -OC 1~6 -alkyl is optionally substituted with one or more halogens. or a pharma- ceutically acceptable salt thereof, or a tautomer or stereoisomer of the compound or a pharma- ceutically acceptable salt thereof, or a mixture of any of the foregoing.

[0204] In certain embodiments, the compound of formula IX has formula IX(a): [ka] or a pharma- ceutically acceptable salt thereof, or a tautomeric compound or a pharma- ceutically acceptable salt thereof, or a mixture of any of the foregoing.

[0205] In certain embodiments, the compound of formula IX has formula IX(b): [ka] or a pharma- ceutically acceptable salt thereof, or a tautomeric compound or a pharma- ceutically acceptable salt thereof, or a mixture of any of the foregoing.

[0206] In certain embodiments, the compound of formula IX is a compound selected from any of the following, a stereoisomer or mixture of stereoisomers thereof, or a pharma- ceutically acceptable salt thereof: [ka] [ka] [ka] [ka] [ka] [ka] [ka] [ka] [ka] [ka] [ka] [ka] [ka] [ka] [ka] [ka] [ka]

[0207] In some embodiments of the methods and uses disclosed herein, the GlyT1 inhibitor is [ka] [In the formula, R 1 teeth, a) a 5- or 6-membered monocyclic heteroaryl having 1, 2, 3, or 4 heteroatoms independently selected from the group consisting of O, N, and S(O)r; b) 5- or 6-membered monocyclic partially saturated heterocycloalkyl having 1, 2, or 3 heteroatoms independently selected from the group consisting of O, N, and S(O)r; and c) O, N and S(O) r A 9- or 10-membered bicyclic heteroaryl having 1, 2, or 3 heteroatoms independently selected from the group consisting of (wherein r is 0, 1 or 2). selected from the group consisting of; wherein each of the groups a), b) and c) is C 1~4 -Alkyl-, C 1~4 -Alkyl-O-, oxetanyl, tetrahydrofuranyl, tetrahydropyranyl, C 3~6 -cycloalkyl- and C 3~6 -cycloalkyl-O-, which in the case of a substituent is attached to the nitrogen ring atom, and said substituent is 1~4 -Alkyl-, C 1~4-Alkyl-CO-, C 3~6 -cycloalkyl- and C 3~6 -cycloalkyl-CO-, Here, the C 1~4 -Alkyl-, C 1~4 -Alkyl-O-, C 1~4 -Alkyl-CO-, oxetanyl, tetrahydrofuranyl, tetrahydropyranyl, C 3~6 -cycloalkyl-, C 3~6 -Cycloalkyl-CO- or C 3~6 Each of the -cycloalkyl-O- substituents is fluoro, -CF 3 , -CHF 2 , -CH 2 optionally substituted with one or more substituents independently selected from the group consisting of F and -CN; R 2 is hydrogen, C 1~4 -Alkyl-, C 1~4 -Alkyl-O-, -CN and C 3~6 -cycloalkyl-, Here, the C 1~4 -Alkyl-, C 1~4 -Alkyl-O- and C 3~6 Each of the -cycloalkyl groups is fluoro, -CF 3 , -CHF 2 , -CH 2 optionally substituted with 1, 2, 3 or more substituents independently selected from the group consisting of F and -CN; R 3 is C 1~6 -Alkyl-O-, C 3~6 -cycloalkyl-O-, morpholino, pyrazolyl, and one oxygen atom and optionally O, N and S(O) as ring members s (wherein s=0, 1, or 2), Here, the C 1~6 -alkyl-O- and the C 3~6-Cycloalkyl-O- is fluoro, -CF 3 , -CHF 2 , -CH 2 F, -CN, C 1~4 -Alkyl-, C 3~6 -cycloalkyl-, C 1~6 -Alkyl-O- and C 3~6 -cycloalkyl-O-; R 4 is hydrogen; or R 3 and R 4 together with the ring atoms of the phenyl group to which they are attached, represent a 4-, 5-, or 6-membered monocyclic partially saturated heterocycloalkyl, or each of which is O, N, and S(O) s (wherein s=0, 1 or 2), wherein in general formula (I), R 3 there must be one ring oxygen atom directly attached to a ring carbon atom of said phenyl group to which is attached; wherein the heterocycloalkyl group is fluoro, -CF 3 , -CHF 2 , -CH 2 F, -CN, C 1~4 -Alkyl-, C 3~6 -cycloalkyl-, C 1~6 -Alkyl-O-, C 3~6 -optionally substituted with 1, 2, 3 or more substituents independently selected from the group consisting of cycloalkyl-O-, oxetanyl-O-, tetrahydrofuranyl-O-, and tetrahydropyranyl-O-; R 5 is hydrogen; R 6 is hydrogen, C 1~4 -Alkyl-SO 2 -, C 3~6 -Cycloalkyl-SO 2 and -CN; R7 is hydrogen; or a)R 6 and R 7 or b) R 6 and R 5 The first pair of phenyl groups, together with the ring atoms of the phenyl groups to which they are attached, are O, N and S(O) u (wherein u=0, 1 or 2), wherein in general formula (I), R 6 one -SO bonded directly to the ring carbon atom of the phenyl group to which is bonded 2 -member must be present, wherein the heterocycloalkyl group is fluoro, -CF 3 , -CHF 2 , -CH 2 F, -CN, C 1~4 -Alkyl-, C 1~6 -Alkyl-O- and C 3~6 -cycloalkyl-O-, optionally substituted with 1, 2, 3 or more substituents independently selected from the group consisting of: or a pharma- ceutically acceptable salt thereof, or a prodrug of the compound or a pharma- ceutically acceptable salt thereof.

[0208] In certain embodiments, the compound of formula X is a compound selected from any of the following, a stereoisomer or mixture of stereoisomers thereof, or a pharma- ceutically acceptable salt thereof: [ka] [ka] [ka] [ka] [ka]

[0209] For example, the compound of formula X can be any diastereomeric mixture or single diastereomer of the following, or a pharma- ceutically acceptable salt thereof. [ka] [ka] [ka] [ka] [ka] [ka] [ka]

[0210] In certain methods and uses disclosed herein, the subject is a subject in need thereof.

[0211] In some embodiments of the uses and methods disclosed herein, the glycine transporter inhibitor, e.g., a GlyT1 inhibitor (e.g., a GlyT1 inhibitor disclosed herein) or a pharma- ceutically acceptable salt thereof, or a prodrug of a glycine transporter inhibitor, e.g., a GlyT1 inhibitor (e.g., a GlyT1 inhibitor disclosed herein) or a pharma- ceutically acceptable salt thereof, is administered in a therapeutically effective amount.

[0212] In some embodiments, the compound or its pharma- ceutically acceptable salt, solvate or prodrug is selected from the compounds described herein. Any of the compounds provided herein can be prepared as a pharma- ceutically acceptable salt, solvate or prodrug, and / or as part of a pharmaceutical composition described in the patents or patent application publications cited herein.

[0213] The compounds described herein may exhibit a specific stereochemistry around a certain atom, for example, cis or trans, but the compounds can also be made in the reverse orientation or in racemic mixtures.Such isomers or racemic mixtures are encompassed in the present disclosure.In addition, the compounds are summarized in the table, but any compound, or its pharmaceutically acceptable salt, solvate or prodrug, can be selected from the table and used in the embodiments provided herein.

[0214] The compounds described herein can be made according to the methods described in the patents or published patent applications cited herein.

[0215] The compounds can be used to inhibit the GlyT1 transporter, and therefore, in some embodiments, the compounds can be referred to as compounds that inhibit the GlyT1 transporter or GlyT1 inhibitors.

[0216] The compounds described herein can be administered in any conventional manner by any route in which they are active.Administration can be systemic, local, or oral.For example, administration can be, but is not limited to, parenteral, subcutaneous, intravenous, intramuscular, intraperitoneal, transdermal, oral, buccal, sublingual, or ophthalmic, or vaginal, by inhalation, by depot injection, or by implant.The method of administration can depend on the condition or disease that is targeted or treated.The selection of the specific route of administration can be selected or adjusted by the clinician according to the method known to the clinician to obtain the desired clinical response.

[0217] In some embodiments, it may be desirable to administer one or more compounds, or its pharma-ceutically acceptable salt, solvate or prodrug locally to the area that needs treatment.This can be achieved, for example, but not limited to, by local injection during surgery, local application, for example, in conjunction with wound dressing after surgery, by injection, by means of catheter, by means of suppository, or by means of implant, where implant is of porous, non-porous or gelatinous material, including membrane such as silastic membrane, or fiber.

[0218] The compounds described herein can be administered alone or in combination with other pharmaceutical agents (either together or sequentially). For example, the compounds can be administered in combination with other drugs, such as for the treatment of EPP, XLPP or CEP. Examples of other pharmaceutical agents or drugs are known to those skilled in the art and include, but are not limited to, those described herein.

[0219] Means and methods for administration are known in the art, and the artisan can refer to various pharmacological reference works for guidance (e.g., Modern Pharmaceutics, Banker & Rhodes, Marcel Dekker, Inc. (1979); and Goodman & See Gilman's The Pharmaceutical Basis of Therapeutics, 6th Edition, MacMillan Publishing Co., New York (1980).

[0220] The amount of compound administered is a therapeutically effective amount.The dosage administered depends on the characteristics of the subject to be treated, such as the specific animal to be treated, age, weight, health, type of concomitant treatment if any, and frequency of treatment, and can be easily determined by those skilled in the art (e.g., by clinicians).Standard dosages for protamine can be used and adjusted (i.e., increased or decreased) according to the above factors.The selection of specific dosage regimen can be selected or adjusted or titrated by clinicians according to methods known to clinicians to obtain desired clinical response.

[0221] The amount of the compounds described herein that is effective in treating and / or preventing a particular disease, condition, or disorder will depend on the nature and extent of the disease, condition, or disorder, and can be determined by standard clinical techniques. In addition, in vitro or in vivo assays may be used as necessary to help identify optimal dosage ranges. The precise dose to be used in the compositions will also depend on the route of administration and the severity of the disorder, and should be determined according to the judgment of the practitioner and each patient's circumstances. However, suitable dosage ranges for oral administration are generally about 0.001 milligrams to about 200 milligrams per kilogram of body weight, about 0.01 milligrams to about 100 milligrams per kilogram of body weight, about 0.01 milligrams to about 70 milligrams per kilogram of body weight, about 0.1 milligrams to about 50 milligrams per kilogram of body weight, 0.5 milligrams to about 20 milligrams per kilogram of body weight, or about 1 milligram to about 10 milligrams per kilogram of body weight. In some embodiments, the oral dose is about 5 milligrams per kilogram of body weight.

[0222] In some embodiments, suitable dosage ranges for intravenous (iv) administration are about 0.01 mg to about 500 mg per kg of body weight, about 0.1 mg to about 100 mg per kg of body weight, about 1 mg to about 50 mg per kg of body weight, or about 10 mg to about 35 mg per kg of body weight. Suitable dosage ranges for other methods of administration can be calculated based on the aforementioned dosages, as known to those of skill in the art. For example, recommended dosages for intranasal, transmucosal, intradermal, intramuscular, intraperitoneal, subcutaneous, epidural, sublingual, intracerebral, intravaginal, transdermal, or inhalation administration are within the range of about 0.001 mg to about 200 mg per kg of body weight, about 0.01 mg to about 100 mg per kg of body weight, about 0.1 mg to about 50 mg per kg of body weight, or about 1 mg to about 20 mg per kg of body weight. Effective doses may be extrapolated from dose-response curves derived from in vitro or animal model test systems. Such animal models and systems are well known in the art.

[0223] The compounds described herein can be formulated for parenteral administration by injection, e.g., by bolus injection or continuous infusion. In some embodiments, the compounds can be administered by continuous subcutaneous infusion over a period of about 15 minutes to about 24 hours. The formulations for injection can be in unit dosage form, e.g., in ampoules or multi-dose containers, with preservatives added as needed. The compositions can take the form of suspensions, solutions or emulsions in oil or aqueous media, and can contain formulating agents such as suspending agents, stabilizers and / or dispersing agents. In some embodiments, the injectables are in the form of short-acting, depot, or implants, and pellets that are injected subcutaneously or intramuscularly. In some embodiments, the parenteral dosage form is in the form of a solution, suspension, emulsion, or dry powder.

[0224] For oral administration, the compounds described herein can be formulated by combining the compounds with pharma- ceutically acceptable carriers known in the art.Such carriers allow the compounds to be formulated as tablets, pills, dragees, capsules, emulsions, liquids, gels, syrups, cachets, pellets, powders, granules, slurries, lozenges, aqueous or oily suspensions, etc., for oral ingestion by the patient to be treated.The pharmaceutical preparations for oral use can be obtained, for example, by adding solid excipients, grinding the mixture obtained as necessary, and processing the mixture of granules after adding suitable auxiliary agents as required to obtain tablets or dragee cores. Suitable excipients include, but are not limited to, bulking agents, such as, but are not limited to, sugars, including lactose, sucrose, mannitol and sorbitol; cellulose preparations, such as, but are not limited to, corn starch, wheat starch, rice starch, potato starch, gelatin, tragacanth gum, methylcellulose, hydroxypropylmethylcellulose, sodium carboxymethylcellulose and polyvinylpyrrolidone (PVP).If desired, disintegrating agents can be added, such as, but are not limited to, cross-linked polyvinylpyrrolidone, agar, or alginic acid or its salts, such as sodium alginate.

[0225] Orally administered compositions can contain one or more optional agents, such as sweeteners, such as fructose, aspartame or saccharin; flavorings, such as peppermint, oil of wintergreen, or cherry; colorings; and preservatives, to provide pharmacologic palatable preparations.Also, when in tablet or pill form, the composition can be coated to delay disintegration and absorption in the digestive tract, thereby providing a sustained action over a long period of time.Selectively permeable membranes surrounding the osmotically active driving compound are also suitable for orally administered compounds.Oral compositions can contain standard vehicles, such as mannitol, lactose, starch, magnesium stearate, sodium saccharin, cellulose, magnesium carbonate, etc.Such vehicles are preferably of pharmaceutical grade.

[0226] Dragee core can be provided with suitable coating.For this purpose, concentrated sugar solution can be used, which can contain gum arabic, talc, polyvinylpyrrolidone, carbopol gel, polyethylene glycol and / or titanium dioxide, lacquer solution, and suitable organic solvent or solvent mixture as required.Dyes or pigments can be added to tablet or dragee coating to identify or characterize different combinations of active compound doses.

[0227] Pharmaceutical preparations that can be used orally include, but are not limited to, push-fit capsules made of gelatin, and sealed soft capsules made of gelatin and plasticizers, such as glycerol or sorbitol. Push-fit capsules can contain active ingredients in a mixture with fillers, such as lactose, binders, such as starch, and / or lubricants, such as talc or magnesium stearate, and optionally stabilizers. In soft capsules, active compounds can be dissolved or suspended in suitable liquids, such as fatty oils, liquid paraffin, or liquid polyethylene glycol. In addition, stabilizers can be added.

[0228] For buccal administration, the compositions may take the form of, for example, tablets or lozenges formulated in conventional manner.

[0229] For administration by inhalation, the compounds described herein can be delivered in the form of aerosol spray presentation from pressurized pack or nebulizer by using suitable propellant, for example, dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide or other suitable gas.In the case of pressurized aerosol, dosage unit can be determined by providing a valve to deliver a measured amount.Capsules and cartridges of, for example, gelatin for use in inhaler or inhaler can be formulated to contain a powder mix of the compound and suitable powder base, for example, lactose or starch.

[0230] The compounds described herein can also be formulated in rectal compositions, such as suppositories or retention enemas, for example, containing conventional suppository bases, such as cocoa butter or other glycerides.The compounds described herein can also be formulated in vaginal compositions, such as vaginal creams, suppositories, pessaries, vaginal rings, and intrauterine devices.

[0231] For transdermal administration, the compound can be applied to a plaster or by a transdermal therapeutic system that is then delivered to the organism. In some embodiments, the compound is present in a cream, a solution, a powder, a liquid emulsion, a liquid suspension, a semisolid, an ointment, a paste, a gel, a jelly, and a foam, or in a patch containing any of the above.

[0232] The compounds described herein can be formulated as depot preparations. Such long-acting formulations can be administered by implantation (e.g., subcutaneously or intramuscularly) or by intramuscular injection. Depot injections can be administered at intervals of about 1 to about 6 months, or longer. Thus, for example, the compounds can be formulated with suitable polymeric or hydrophobic materials (e.g., as an emulsion in an acceptable oil), or ion exchange resins, or as sparingly soluble derivatives, e.g., as sparingly soluble salts.

[0233] In some embodiments, the compound can be delivered in a controlled release system. In one embodiment, a pump can be used (see Langer, supra; Sefton, CRC Crit. Ref. Biomed. Eng., 1987, 14, 201; Buchwald et al., Surgery, 1980, 88, 507; Saudek et al., N. Engl. J. Med., 1989, 321, 574). In some embodiments, polymeric materials can be used (Medical Applications of See, Controlled Release, Langer and Wise (eds.), CRC Pres., Boca Raton, Fla. (1974); Controlled Drug Bioavailability, Drug Product Design and Performance, Smolen and Ball (eds.), Wiley, New York (1984); Ranger et al., J. Macromol. Sci. Rev. Macromol. Chem., 1983, 23, 61; also Levy et al., Science, 1985, 228, 190; During et al., Ann. Neurol., 1989, 25, 351; see also Howard et al., J. Neurosurg., 1989, 71, 105. In yet another embodiment, the controlled release system can be placed in close proximity to the target of the compounds described herein, such as the liver, thus requiring only a small systemic dose (see, e.g., Goodson, in Medical Applications of Controlled Release, supra, vol. 2, pp. 115-138 (1984). Other controlled release systems discussed in the review by Langer, Science, 1990, 249, 1527-1533 may also be used.

[0234] It is also known in the art that the compound may be included in such formulations with pharma- ceutically acceptable diluents, bulking agents, disintegrants, binders, lubricants, surfactants, hydrophobic media, aqueous media, emulsifiers, buffers, humectants, solubilizers, preservatives, etc. The pharmaceutical composition may also include suitable solid- or gel-phase carriers or excipients. Examples of such carriers or excipients include, but are not limited to, calcium carbonate, calcium phosphate, various sugars, starches, cellulose derivatives, gelatin, and polymers such as polyethylene glycol. In some embodiments, the compounds described herein may be used with agents including, but not limited to, topical analgesics (e.g., lidocaine), barrier devices (e.g., GelClair) or rinses (e.g., Caphosol).

[0235] In some embodiments, the compounds described herein can be delivered in a vehicle, in particular a liposome (see Langer, Science, 1990, 249, 1527-1533; Treat et al., in Liposomes in the Therapy of Infectious Disease and Cancer, Lopez-Berestein and Fidler (eds.), Liss, New York, pp. 353-365 (1989); Lopez-Berestein, ibid, pp. 317-327; see generally, ibid.).

[0236] Suitable compositions include, but are not limited to, oral non-absorbable compositions. Suitable compositions also include, but are not limited to, saline, water, cyclodextrin solutions, and buffers of pH 3-9.

[0237] The compounds described herein, or their pharma- ceutically acceptable salts, solvates or prodrugs, can be formulated with a number of excipients, including, but not limited to, purified water, propylene glycol, PEG400, glycerin, DMA, ethanol, benzyl alcohol, citric acid / sodium citrate (pH 3), citric acid / sodium citrate (pH 5), tris(hydroxymethyl)aminomethane HCl (pH 7.0), 0.9% saline, and 1.2% saline, and any combination thereof.In some embodiments, the excipient is selected from propylene glycol, purified water and glycerin.

[0238] In some embodiments, the formulation can be lyophilized to a solid and reconstituted, for example, with water prior to use.

[0239] When administered to mammals (eg, animals for veterinary use or humans for clinical use), the compounds can be administered in isolated form.

[0240] When administered to humans, the compound can be sterile. When the compound of formula I-VIII is administered intravenously, water is a suitable carrier. Saline solution and aqueous dextrose and glycerol solutions can also be used as liquid carriers, especially for injectable solutions. Suitable pharmaceutical carriers also include excipients such as starch, glucose, lactose, sucrose, gelatin, malt, rice, flour, chalk, silica gel, sodium stearate, glycerol monostearate, talc, sodium chloride, dried skim milk, glycerol, propylene glycol, water, ethanol, and the like. The composition can also contain minor amounts of wetting or emulsifying agents, or pH buffering agents, if desired.

[0241] The compositions described herein can take the form of a solution, suspension, emulsion, tablet, pill, pellet, capsule, capsule containing liquid, powder, sustained release formulation, suppository, aerosol, spray, or any other form suitable for use.Examples of suitable pharmaceutical carriers are described in Remington's Pharmaceutical Sciences, AR Gennaro (Editor) Mack Publishing Co.

[0242] In some embodiments, the compound is formulated according to routine procedures as a pharmaceutical composition suitable for administration to humans. Typically, the compound is a solution in a sterile isotonic aqueous buffer. If necessary, the composition can also include a solubilizing agent. Compositions for intravenous administration may optionally include a local anesthetic, such as lidocaine, to ease pain at the site of injection. Generally, the ingredients are supplied in unit dosage form, either separately or mixed together, for example, as a dry lyophilized powder or water-free concentrate in a hermetically sealed container, such as an ampoule or sachet indicating the amount of active agent. If the compound is administered by injection, it can be dispensed, for example, in an infusion bottle containing sterile pharmaceutical grade water or saline. If the compound is administered by injection, an ampoule of sterile water for injection or saline can be provided so that the ingredients can be mixed prior to administration.

[0243] Pharmaceutical composition can be in unit dosage form.In such form, composition can be divided into unit doses containing appropriate amount of active components.Unit dosage form can be packaged preparation, package containing discrete amount of preparation, for example, packeted tablets, capsules, and powders in vials or ampoules.Unit dosage form can also be capsules, cachets or tablets themselves, or it can be any of these packaged forms in appropriate number.

[0244] In some embodiments, the composition is in the form of a liquid, where the active agent (i.e., one of the facially amphiphilic polymers or oligomers disclosed herein) is present in a solution, in a suspension, as an emulsion, or as a solution / suspension. In some embodiments, the liquid composition is in the form of a gel. In other embodiments, the liquid composition is aqueous. In other embodiments, the composition is in the form of an ointment.

[0245] In some embodiments, the composition is in the form of a solid article.For example, in some embodiments, the ophthalmic composition is a solid article, which can be inserted into a suitable location in the eye, for example between the eye and the eyelid or the conjunctival sac, and releases active agent there, for example, as described in U.S. Pat. No. 3,863,633; U.S. Pat. No. 3,867,519; U.S. Pat. No. 3,868,445; U.S. Pat. No. 3,960,150; U.S. Pat. No. 3,963,025; U.S. Pat. No. 4,186,184; U.S. Pat. No. 4,303,637; U.S. Pat. No. 5,443,505; and U.S. Pat. No. 5,869,079.The release from such an article is usually to the cornea, either through the tear fluid that bathes the surface of the cornea, or directly to the cornea itself, and the solid article is generally in intimate contact.The solid article suitable for implantation into the eye in such a way is generally composed mainly of polymers, and can be biodegradable or non-biodegradable. Biodegradable polymers that can be used in the preparation of ocular implants carrying one or more compounds include, but are not limited to, aliphatic polyesters such as poly(glycolide), poly(lactide), poly(epsilon-caprolactone), poly-(hydroxybutyrate) and poly(hydroxyvalerate) polymers and copolymers, polyamino acids, polyorthoesters, polyanhydrides, aliphatic polycarbonates, and polyether lactones. Suitable non-biodegradable polymers include silicone elastomers.

[0246] The compositions described herein can contain preservative.Suitable preservative includes, but is not limited to, mercury-containing substances, such as phenylmercury salts (such as phenylmercuric acetate, phenylmercuric borate and phenylmercuric nitrate) and thimerosal; stabilized chlorine dioxide; quaternary ammonium compounds, such as benzalkonium chloride, cetyltrimethylammonium bromide and cetylpyridinium chloride; imidazolidinyl urea; parabens, such as methylparaben, ethylparaben, propylparaben and butylparaben, and their salts; phenoxyethanol; chlorophenoxyethanol; phenoxypropanol; chlorobutanol; chlorocresol; phenylethyl alcohol; disodium EDTA; and sorbic acid and its salts.

[0247] Optionally, one or more stabilizers may be included in the composition to enhance chemical stability, if necessary. Suitable stabilizers include, but are not limited to, chelating or complexing agents, such as the calcium complexing agent ethylenediaminetetraacetic acid (EDTA). For example, a suitable amount of EDTA, or a salt thereof, such as the disodium salt, may be included in the composition to complex excess calcium ions and prevent gel formation during storage. EDTA or a salt thereof may be suitably included in an amount of about 0.01% to about 0.5%. In those embodiments containing a preservative other than EDTA, EDTA or a salt thereof, more specifically, disodium EDTA, may be present in an amount of about 0.025% to about 0.1% by weight.

[0248] One or more antioxidants may also be included in the composition.Suitable antioxidants include, but are not limited to, ascorbic acid, sodium metabisulfite, sodium bisulfite, acetylcysteine, polyquaternium-1, benzalkonium chloride, thimerosal, chlorobutanol, methylparaben, propylparaben, phenylethyl alcohol, edetate disodium, sorbic acid, or other agents known to those skilled in the art.Such preservatives are typically used at a level of about 0.001% to about 1.0% by weight.

[0249] In some embodiments, the compound is solubilized, at least in part, by an acceptable solubilizing agent. Certain acceptable non-ionic surfactants, such as polysorbate 80, can be useful as solubilizing agents, as can ophthalmically acceptable glycols, polyglycols, such as polyethylene glycol 400 (PEG-400), and glycol ethers.

[0250] A suitable solubilizer for the solution and solution / suspension compositions is cyclodextrin. Suitable cyclodextrins can be selected from α-cyclodextrin, β-cyclodextrin, γ-cyclodextrin, alkyl cyclodextrins (e.g., methyl-β-cyclodextrin, dimethyl-β-cyclodextrin, diethyl-β-cyclodextrin), hydroxyalkyl cyclodextrins (e.g., hydroxyethyl-β-cyclodextrin, hydroxypropyl-β-cyclodextrin), carboxy-alkyl cyclodextrins (e.g., carboxymethyl-β-cyclodextrin), sulfoalkyl ether cyclodextrins (e.g., sulfobutyl ether-β-cyclodextrin), and the like. Ophthalmic applications of cyclodextrins are reviewed in Rajewski et al., Journal of Pharmaceutical Sciences, 1996, 85, 1155-1159. do.

[0251] In some embodiments, the composition optionally contains a suspending agent.For example, in those embodiments where the composition is an aqueous suspension or solution / suspension, the composition can contain one or more polymers as a suspending agent.Useful polymers include, but are not limited to, water-soluble polymers, such as cellulose polymers, such as hydroxypropylmethylcellulose, and water-insoluble polymers, such as crosslinked carboxy-containing polymers.

[0252] One or more acceptable pH adjusting agents and / or buffers may be included in the composition, including acids such as acetic acid, boric acid, citric acid, lactic acid, phosphoric acid and hydrochloric acid; bases such as sodium hydroxide, sodium phosphate, sodium borate, sodium citrate, sodium acetate, sodium lactate and tris-hydroxymethylaminomethane; and buffers such as citrate / dextrose, sodium bicarbonate and ammonium chloride. Such acids, bases and buffers are included in the amount necessary to maintain the pH of the composition in an acceptable range.

[0253] One or more acceptable salts, solvents or prodrugs can be included in the composition in the amount necessary to make the osmolality of the composition acceptable.Such salts include, but are not limited to, those with sodium, potassium or ammonium cations and chloride, citrate, ascorbic acid, borate, phosphate, bicarbonate, sulfate, thiosulfate or bisulfate anions.In some embodiments, the salt includes sodium chloride, potassium chloride, sodium thiosulfate, sodium bisulfite and ammonium sulfate.In some embodiments, the salt is sodium chloride.

[0254] Optionally, one or more acceptable surfactants, such as, but not limited to, non-ionic surfactants, or co-solvents, may be included in the composition to enhance the solubility of the components of the composition, or to impart physical stability, or for other purposes. Suitable non-ionic surfactants include, but are not limited to, polyoxyethylene fatty acid glycerides and vegetable oils, such as polyoxyethylene (60) hydrogenated castor oil; and polyoxyethylene alkyl ethers and alkyl phenyl ethers, such as octoxynol 10, octoxynol 40; polysorbate 20, 60 and 80; polyoxyethylene / polyoxypropylene surfactants (e.g., Pluronic® F-68, F84 and P-103); cyclodextrins; or other agents known to those skilled in the art. Typically, such co-solvents or surfactants are used in the composition at a level of about 0.01% to about 2% by weight.

[0255] In some embodiments, a pharmaceutical pack or kit is provided that includes one or more containers filled with one or more compounds as described herein.Optionally, associated with such containers can be a notice in the form prescribed by the government agency that regulates the manufacture, use or sale of pharmaceuticals or biological products, which reflects the approval by the agency of manufacture, use or sale for human administration to treat conditions, diseases or disorders as described herein.In some embodiments, the kit contains more than one compound as described herein.In some embodiments, the kit includes the compound as described herein in a single injectable dosage form, for example, a single dose in an injection device such as a needle-equipped syringe.

[0256] In some embodiments, the method comprises administering to a subject one or more compounds as described herein, or its pharma-ceutically acceptable salt, solvate or prodrug, or its pharmaceutical composition.In some embodiments, the subject is a subject who needs such treatment.As described herein, in some embodiments, the subject is a mammal, for example, but not limited to, a human.

[0257] In some embodiments, there is also provided one or more compounds as described above, or pharma- ceutically acceptable salts, solvates or prodrugs thereof, or a pharmaceutical composition comprising one or more compounds as described above, for use in the manufacture of a medicament for the treatment of EPP, XLPP or CEP, or their associated syndromes, including but not limited to, the conditions described herein, in a subject, such as those described herein, in a method of treating and / or preventing. In some embodiments, the subject is a subject in need thereof.

[0258] The present embodiments also provide for the use of one or more of the compounds described above, or a pharma- ceutically acceptable salt, solvate or prodrug thereof, or a pharmaceutical composition comprising one or more of the compounds described above, in inhibiting GlyT1 transporters, e.g., those present on the surface of a cell. In some embodiments, the compound, a pharma- ceutically acceptable salt thereof, or a pharmaceutical composition thereof inhibits internalization, transport and / or degradation of the GlyT1 transporter.

[0259] As used herein, "inhibition" can refer to the inhibition of any specific activity. The activity of the GlyT1 transporter can be measured by any method known in the art, including, but not limited to, the methods described herein.

[0260] The compounds described herein are inhibitors of the GlyT1 transporter. The ability of a compound to inhibit GlyT1 transporter activity can be measured using any assay known in the art.

[0261] In general, assays for testing compounds that inhibit GlyT1 transporter activity include the determination of any parameter that is indirectly or directly influenced by the GlyT1 transporter, such as a functional, physical or chemical effect.

[0262] Samples or assays containing the GlyT1 transporter that are treated with a potential inhibitor are compared to a control sample without the inhibitor to determine the degree of inhibition. The control sample (untreated with the inhibitor) is assigned a relative GlyT1 transporter activity value of 100%. Inhibition of the GlyT1 transporter is achieved when the GlyT1 transporter activity value relative to the control is about 80%, 50% or 25%.

[0263] Ligand binding to the GlyT1 transporter can be tested in several formats. Binding can be performed in solution, in a bilayer membrane, bound to a solid phase, in a lipid monolayer, or in a vesicle. For example, in an assay, the binding of a natural ligand to the transporter is measured in the presence of a candidate modulator, such as a compound described herein. Alternatively, the binding of a candidate modulator may be measured in the presence of a natural ligand. Often, a competitive assay is used that measures the ability of a compound to compete with the binding of a natural ligand to the transporter. Binding can be tested, for example, by measuring changes in spectroscopic properties (e.g., fluorescence, absorption, refractive index), hydrodynamic (e.g., shape), or chromatographic or solubility properties.

[0264] After the transporter is expressed in the cells, the cells can be grown in a suitable medium in a suitable cell plate. The cells can be plated, for example, at 5000-10000 cells per well in a 384-well plate. In some embodiments, the cells are plated at about 1000, 2000, 3000, 4000, 5000, 6000, 7000, 8000, 9000 or 10000 cells per well. The plate can have any number of wells and the number of cells can be modified accordingly.

[0265] Any pharmaceutical agent having utility in the applications described herein can be used in combination therapy, co-administration or co-formulation with the above compositions. Thus, the compounds described herein can be administered either before, together with, or after such therapeutic agents are administered to the subject.

[0266] The additional pharmaceutical agents may be administered in combination therapy (including co-formulation) with one or more compounds described herein.

[0267] In some embodiments, the response of the disease or disorder to treatment is monitored, and the treatment regimen is adjusted, if necessary, in light of such monitoring.

[0268] The frequency of administration is typically such that the dosing interval, e.g., the period between one dose and the next during waking hours, is about 1 to about 24 hours, about 2 to about 12 hours, about 3 to about 8 hours, or about 4 to about 6 hours. In some embodiments, doses are administered 1, 2, 3 or 4 times daily. An appropriate dosing interval is determined so that the selected composition is capable of maintaining a concentration of the compound in the subject and / or target tissue (e.g., EC 50 It will be appreciated by those of skill in the art that the duration of time that the EC50 concentration is greater than the lowest concentration of compound that inhibits transporter activity by 90% will depend in part on the length of time that the EC50 concentration is greater than the lowest concentration of compound that inhibits transporter activity by 90%. Ideally, the concentration will be greater than the EC50 concentration for at least 100% of the dosing interval. 50 If this is not achievable, concentrations should remain above the EC 50 or remain above the EC for at least approximately 40% of the dosing interval. 50 It is desirable that the How to use

[0269] The present application provides a method for preventing or treating a disorder associated with accumulation of PPIX in a subject, comprising administering to the subject one or more glycine transporter inhibitors or their pharma- ceutically acceptable salts, or one or more prodrugs of glycine transporter inhibitors or their pharma- ceutically acceptable salts.In certain embodiments, the glycine transporter inhibitor is a GlyT1 inhibitor, for example, the GlyT1 inhibitor disclosed herein.For example, the present application provides a method for preventing or treating a disorder associated with accumulation of PPIX in a subject, comprising administering to the subject one or more glycine transporter inhibitors or their pharma- ceutically acceptable salts. [ka] or a pharma- ceutically acceptable salt thereof, or a prodrug of bitopertin or a pharma- ceutically acceptable salt thereof, to a subject.

[0270] In part, the disclosure relates to a method of treating erythropoietic protoporphyria (EPP), X-linked protoporphyria (XLPP), or congenital erythropoietic porphyria (CEP) in a subject, comprising administering to the subject a pharmaceutical composition comprising one or more glycine transporter inhibitors (e.g., GlyT1 inhibitors) or a pharmaceutically acceptable salt thereof, or one or more prodrugs of a glycine transporter inhibitor (e.g., GlyT1 inhibitors) or a salt thereof. In certain embodiments, the disclosure provides a method of preventing, treating, or reducing the rate of progression and / or severity of one or more complications of EPP, XLPP, or CEP in a subject, comprising administering to the subject a pharmaceutical composition comprising one or more glycine transporter inhibitors (e.g., GlyT1 inhibitors) or a pharmaceutically acceptable salt thereof, or one or more prodrugs of a glycine transporter inhibitor (e.g., GlyT1 inhibitors) or a pharmaceutically acceptable salt thereof. These methods are particularly aimed at therapeutic and prophylactic treatment of animals, more particularly humans. The terms "subject", "individual" or "patient" are used interchangeably throughout this specification and refer to either human or non-human animals. These terms include mammals, such as humans, non-human primates, laboratory animals, livestock animals (including cows, pigs, camels, etc.), companion animals (e.g., dogs, cats, other domestic animals, etc.) and rodents (e.g., mice and rats). In certain embodiments, the patient, subject or individual is a human.

[0271] The present application provides a method for preventing or treating erythropoietic protoporphyria (EPP), X-linked protoporphyria (XLPP) or congenital erythropoietic porphyria (CEP), or their associated syndromes (e.g., EPP-related syndromes, XLPP-related syndromes or CEP-related syndromes) in a subject, comprising administering to the subject one or more glycine transporter inhibitors or pharma- ceutically acceptable salts thereof, or one or more prodrugs of glycine transporter inhibitors or pharma- ceutically acceptable salts thereof. The present application further provides a method for preventing or treating EPP, XLPP or CEP in a subject, comprising administering to the subject one or more glycine transporter inhibitors or pharma- ceutically acceptable salts thereof, or one or more prodrugs of glycine transporter inhibitors or pharma- ceutically acceptable salts thereof. For example, the present application provides a method for treating EPP, XLPP or CEP in a subject, comprising administering to the subject one or more glycine transporter inhibitors or their pharmaceutically acceptable salts, or one or more prodrugs of glycine transporter inhibitors or their pharmaceutically acceptable salts.In some embodiments, the one or more glycine transporter inhibitors are one or more GlyT1 and / or GlyT2 inhibitors.In some embodiments, the one or more glycine transporter inhibitors are one or more GlyT1 inhibitors, for example, one or more GlyT1 inhibitors disclosed herein.For example, the present application provides a method for preventing or treating EPP, XLPP or CEP in a subject, comprising administering to the subject bitopertin or its pharmaceutically acceptable salts, or prodrugs of bitopertin or its pharmaceutically acceptable salts.

[0272] The present application further provides a method of preventing or treating EPP, XLPP or CEP, or their associated syndromes (e.g., EPP-related syndromes, XLPP-related syndromes or CEP-related syndromes) in a subject, comprising administering to the subject a pharmaceutical composition comprising one or more glycine transporter inhibitors or pharma- ceutically acceptable salts thereof, or one or more prodrugs of glycine transporter inhibitors or pharma- ceutically acceptable salts thereof. The present application further provides a method of preventing or treating EPP, XLPP or CEP in a subject, comprising administering to the subject a pharmaceutical composition comprising one or more glycine transporter inhibitors or pharma- ceutically acceptable salts thereof, or one or more prodrugs of glycine transporter inhibitors or pharma- ceutically acceptable salts thereof. For example, the present application provides a method for treating EPP, XLPP or CEP in a subject, comprising administering to the subject a pharmaceutical composition comprising one or more glycine transporter inhibitors or their pharmaceutically acceptable salts, or one or more prodrugs of glycine transporter inhibitors or their pharmaceutically acceptable salts.In some embodiments, the one or more glycine transporter inhibitors are one or more GlyT1 and / or GlyT2 inhibitors.In some embodiments, the one or more glycine transporter inhibitors are one or more GlyT1 inhibitors, for example, one or more GlyT1 inhibitors disclosed herein.In certain embodiments of the above, the pharmaceutical composition further comprises a pharmaceutically acceptable carrier. For example, the application provides a method for preventing or treating EPP, XLPP, or CEP in a subject, comprising administering to the subject a pharmaceutical composition comprising bitopertin or a pharma- ceutically acceptable salt thereof, or a prodrug of bitopertin or a pharma- ceutically acceptable salt thereof, wherein the pharmaceutical composition further comprises a pharma- ceutically acceptable carrier.

[0273] Erythropoietic protoporphyria (EPP) and X-linked protoporphyria (XLPP) are erythropoietic cutaneous porphyrias characterized by acute non-bullous photosensitivity, intolerance to sunlight and a marked reduction in quality of life. EPP is caused by a partial deficiency in ferrochelatase (FECH), which catalyzes the final step in the heme biosynthetic pathway. Deficiency of FECH increases the levels of metal-free erythrocyte PPIX (also referred to herein as "free protoporphyrin IX" and "PPIX"). XLPP is typically caused by a C-terminal deletion in the ALAS2 gene that results in a gain-of-function mutation. These gain-of-function mutations increase the enzymatic activity of ALAS2, causing the accumulation of both metal-free and zinc-bound PPIX. Both EPP and XLPP result in the accumulation of PPIX in red blood cells and other tissues or biological fluids (e.g., skin, liver, bile or feces). PPIX, which is lipid soluble and excreted via the bile, is hepatotoxic at high concentrations.

[0274] Patients with EPP or XLPP usually develop photosensitivity during early childhood. Patients frequently present with symptoms of burning, itching, pain, erythema and edema in areas exposed to sunlight. Skin symptoms may be associated with abnormal liver enzyme activity, hepatobiliary damage, such as jaundice and cirrhosis, iron deficiency and corresponding microcytic anemia.

[0275] The diagnosis of EPP and XLPP can be determined by measuring the levels of total red blood cells, free protoporphyrin IX and zinc protoporphyrin IX in hemolyzed anticoagulated whole blood. The diagnosis of EPP and / or XLPP can be made based on the increased levels of free protoporphyrin IX in the blood. Patients with XLPP have a significantly higher ratio of zinc protoporphyrin IX to free protoporphyrin IX (e.g., >25%) compared to patients with EPP (e.g., ≦15%).

[0276] The diagnosis of EPP can also be determined by measuring the level of ferrochelatase activity in a subject. Ferrochelatase is a mitochondrial enzyme that catalyzes the insertion of ferrous iron into PPIX to form heme. Ferrochelatase also catalyzes the insertion of zinc from any PPIX to form zinc protoporphyrin IX (ZPPIX), which remains intact after the end of heme synthesis. In EPP, the formation of both heme and ZPPIX is impaired, so free PPIX accumulates in bone marrow reticulocytes. In some embodiments, the present disclosure relates to methods of treating a subject whose ferrochelatase activity level is reduced to 10-35% of the ferrochelatase activity level observed in a normal subject. In some embodiments, the present disclosure relates to methods of treating a subject whose ferrochelatase activity level is reduced to less than 50% of the ferrochelatase activity level observed in a normal subject.

[0277] XLPP has a similar phenotype to EPP and can be identified based on genetic analysis of ALAS2 or by determining the enzyme activity level of ALAS2. In some embodiments, the disclosure relates to a method of treating a subject with a gain-of-function mutation in ALAS2. In some embodiments, the subject's ALAS2 enzyme activity is increased. Because ferrochelatase is not deficient in XLPP, a portion of the excess PPIX measured in red blood cells is ZPPIX, and a lower percentage (e.g., 50-85%) is metal-free. In some embodiments, the subject has increased zinc protoporphyrin IX levels in red blood cells. In some embodiments, the method reduces zinc protoporphyrin IX levels in the subject's red blood cells. In some embodiments, the method reduces zinc protoporphyrin IX levels in the subject's red blood cells by at least 10% (e.g., 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or at least 100%).

[0278] In certain aspects, the present disclosure relates to a method for treating erythropoietic protoporphyria (EPP) and / or X-linked protoporphyria (XLPP) in a subject, comprising administering to the subject a pharmaceutical composition comprising one or more glycine transporter inhibitors (e.g., GlyT1 inhibitors) or pharma- ceutically acceptable salts thereof, or one or more prodrugs or salts thereof of glycine transporter inhibitors (e.g., GlyT1 inhibitors), wherein the subject has an increased PPIX level. In some embodiments, the method relates to a subject having a PPIX level that is at least 10%, 20%, 30%, 40% or 50% higher than the PPIX level in a healthy subject before administration of a glycine transporter inhibitor (e.g., GlyT1 inhibitor). In some embodiments, the method relates to a subject having a PPIX level that is at least 10% higher than the PPIX level in a healthy subject before administration of a glycine transporter inhibitor (e.g., GlyT1 inhibitor). In some embodiments, the method relates to a subject having a PPIX level at least 20% higher than the PPIX level in a healthy subject before administration of a glycine transporter inhibitor (e.g., a GlyT1 inhibitor). In some embodiments, the method relates to a subject having a PPIX level at least 30% higher than the PPIX level in a healthy subject before administration of a glycine transporter inhibitor (e.g., a GlyT1 inhibitor). In some embodiments, the method relates to a subject having a PPIX level at least 40% higher than the PPIX level in a healthy subject before administration of a glycine transporter inhibitor (e.g., a GlyT1 inhibitor). In some embodiments, the method relates to a subject having a PPIX level at least 50% higher than the PPIX level in a healthy subject before administration of a glycine transporter inhibitor (e.g., a GlyT1 inhibitor). In some embodiments, the subject has an increased protoporphyrin IX level in feces. In some embodiments, the subject has an increased protoporphyrin IX level in the skin. In some embodiments, the subject has an increased free protoporphyrin IX level in red blood cells. In some embodiments, the subject has a protoporphyrin IX level in red blood cells greater than 31 μmol L −1 .In some embodiments, the subject has a red blood cell protoporphyrin IX level between 31 μmol L −1 and 53 μmol L −1 . In some embodiments, the subject has a red blood cell protoporphyrin IX level greater than 53 μmol L −1 .

[0279] The present application further provides a method of inhibiting PPIX synthesis in vivo, comprising administering to a subject a glycine transporter inhibitor or a pharma- ceutically acceptable salt thereof, or one or more prodrugs of a glycine transporter inhibitor or a pharma- ceutically acceptable salt thereof. In certain aspects, the present disclosure relates to a method of inhibiting PPIX synthesis in vivo, comprising administering to a subject a glycine transporter inhibitor (e.g., a GlyT1 inhibitor) or a pharma- ceutically acceptable salt thereof, or a prodrug of a glycine transporter inhibitor (e.g., a GlyT1 inhibitor) or a pharma- ceutically acceptable salt thereof. In some embodiments, the present disclosure relates to a method of inhibiting PPIX synthesis in vivo by at least 10% (e.g., 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or at least 100%). In some embodiments, the present disclosure relates to a method of inhibiting PPIX synthesis in vivo by at least 20%. In some embodiments, the present disclosure relates to a method of inhibiting PPIX synthesis in vivo by at least 30%. In some embodiments, the disclosure relates to a method of inhibiting PPIX synthesis in vivo by at least 40%. In some embodiments, the disclosure relates to a method of inhibiting PPIX synthesis in vivo by at least 50%. In some embodiments, the disclosure relates to a method of inhibiting PPIX synthesis in vivo by at least 60%. In some embodiments, the disclosure relates to a method of inhibiting PPIX synthesis in vivo by at least 70%. In some embodiments, the disclosure relates to a method of inhibiting PPIX synthesis in vivo by at least 80%. In some embodiments, the disclosure relates to a method of inhibiting PPIX synthesis in vivo by at least 90%. In some embodiments, the disclosure relates to a method of inhibiting PPIX synthesis in vivo by at least 100%. The present application further provides a method of decreasing a rate of PPIX synthesis in vivo, comprising administering to a subject a glycine transporter inhibitor or a pharma- ceutically acceptable salt thereof, or one or more prodrugs of a glycine transporter inhibitor or a pharma- ceutically acceptable salt thereof.In certain embodiments of the methods and uses disclosed herein, directly or indirectly inhibit PPIX accumulation.In certain such embodiments, PPIX accumulation is inhibited in a dose-dependent manner.In certain embodiments of the above-mentioned methods, the glycine transporter inhibitor is a GlyT1 inhibitor, for example, a GlyT1 inhibitor disclosed herein.For example, the present application provides a method of inhibiting PPIX synthesis in vivo, a method of reducing the rate of PPIX synthesis in vitro, and / or a method of inhibiting PPIX accumulation in vivo, comprising administering to a subject bitopertin or a pharma-ceutically acceptable salt thereof, or a prodrug of bitopertin or a pharma-ceutically acceptable salt thereof.

[0280] In some embodiments, the method relates to a method for reducing free protoporphyrin IX levels in a subject. In some embodiments, the method relates to a method for reducing free protoporphyrin IX levels in red blood cells of a subject. In some embodiments, the method reduces protoporphyrin IX levels in red blood cells of a subject to a level less than 53 μmol L-1. In some embodiments, the method reduces protoporphyrin IX levels in red blood cells of a subject to a level less than 31 μmol L-1. In some embodiments, the method reduces protoporphyrin IX levels in red blood cells of a subject to a level less than 15 μmol L-1. In some embodiments, the method relates to reducing protoporphyrin IX levels in feces of a subject. In some embodiments, the method reduces protoporphyrin IX levels in skin of a subject. In some embodiments, the method relates to a method for reducing free protoporphyrin IX levels in a subject by at least 10% (e.g., 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or at least 100%). In some embodiments, the method relates to a method for reducing free protoporphyrin IX levels in a subject by at least 15%. In some embodiments, the method relates to a method for reducing free protoporphyrin IX levels in a subject by at least 20%. In some embodiments, the method relates to a method for reducing free protoporphyrin IX levels in a subject by at least 25%. In some embodiments, the method relates to a method for reducing free protoporphyrin IX levels in a subject by at least 30%. In some embodiments, the method relates to a method for reducing free protoporphyrin IX levels in a subject by at least 35%. In some embodiments, the method relates to a method for reducing free protoporphyrin IX levels in a subject by at least 40%, hi some embodiments, the method relates to a method for reducing free protoporphyrin IX levels in a subject by at least 45%, hi some embodiments, the method relates to a method for reducing free protoporphyrin IX levels in a subject by at least 50%.In some embodiments, the method relates to a method for reducing free protoporphyrin IX levels in a subject by at least 55%. In some embodiments, the method relates to a method for reducing free protoporphyrin IX levels in a subject by at least 60%. In some embodiments, the method relates to a method for reducing free protoporphyrin IX levels in a subject by at least 65%. In some embodiments, the method relates to a method for reducing free protoporphyrin IX levels in a subject by at least 70%. In some embodiments, the method relates to a method for reducing free protoporphyrin IX levels in a subject by at least 75%. In some embodiments, the method relates to a method for reducing free protoporphyrin IX levels in a subject by at least 80%. In some embodiments, the method relates to a method for reducing free protoporphyrin IX levels in a subject by at least 85%. In some embodiments, the method relates to a method for reducing free protoporphyrin IX levels in a subject by at least 90%. In some embodiments, the method relates to a method for reducing free protoporphyrin IX levels in a subject by at least 95%. In some embodiments, the method relates to a method for reducing free protoporphyrin IX levels in a subject by at least 100%.

[0281] In certain aspects, the present disclosure relates to a method of treating X-linked protoporphyria (XLPP) in a subject, comprising administering to the subject a pharmaceutical composition comprising one or more glycine transporter inhibitors (e.g., GlyT1 inhibitors) or pharma- ceutically acceptable salts thereof, or one or more prodrugs or salts thereof of glycine transporter inhibitors (e.g., GlyT1 inhibitors), wherein the subject has an increased zinc protoporphyrin IX (ZPPIX) level. In some embodiments, the method relates to a subject having a ZPPIX level that is at least 10%, 20%, 30%, 40% or 50% higher than the ZPPIX level in a healthy subject before administration of a glycine transporter inhibitor (e.g., GlyT1 inhibitor). In some embodiments, the method relates to a subject having a ZPPIX level that is at least 10% higher than the ZPPIX level in a healthy subject before administration of a glycine transporter inhibitor (e.g., GlyT1 inhibitor). In some embodiments, the method relates to a subject having a ZPPIX level at least 20% higher than the ZPPIX level in a healthy subject before administration of a glycine transporter inhibitor (e.g., a GlyT1 inhibitor). In some embodiments, the method relates to a subject having a ZPPIX level at least 30% higher than the ZPPIX level in a healthy subject before administration of a glycine transporter inhibitor (e.g., a GlyT1 inhibitor). In some embodiments, the method relates to a subject having a ZPPIX level at least 40% higher than the ZPPIX level in a healthy subject before administration of a glycine transporter inhibitor (e.g., a GlyT1 inhibitor). In some embodiments, the method relates to a subject having a ZPPIX level at least 50% higher than the ZPPIX level in a healthy subject before administration of a glycine transporter inhibitor (e.g., a GlyT1 inhibitor). In some embodiments, the subject has an increased ZPPIX level in red blood cells.

[0282] In certain aspects, the present disclosure relates to a method of treating X-linked protoporphyria (XLPP) in a subject, comprising administering to the subject a pharmaceutical composition comprising one or more glycine transporter inhibitors (e.g., GlyT1 inhibitors) or pharma- ceutically acceptable salts thereof, or one or more prodrugs or salts thereof of glycine transporter inhibitors (e.g., GlyT1 inhibitors), wherein the subject has an increased ratio of zinc protoporphyrin IX (ZPPIX) to free protoporphyrin IX (ZPPIX / PPIX ratio) compared to a subject with EPP. In some embodiments, the method relates to a subject with a ZPPIX / PPIX ratio of at least 15% (e.g., 15%, 20%, 25%, 30%, 35%, 40% or 45%). In some embodiments, the method relates to a subject with a ZPPIX / PPIX ratio of at least 20%. In some embodiments, the method relates to a subject with a ZPPIX / PPIX ratio of at least 25%. In some embodiments, the method relates to a subject having a ZPPIX / PPIX ratio of at least 30%. In some embodiments, the method relates to a subject having a ZPPIX / PPIX ratio of at least 35%. In some embodiments, the method relates to a subject having a ZPPIX / PPIX ratio of at least 40%. In some embodiments, the method relates to a subject having a ZPPIX / PPIX ratio of at least 45%.

[0283] In certain aspects, the present disclosure relates to a method of inhibiting zinc protoporphyrin IX (ZPPIX) synthesis in vivo, comprising administering to a subject a GlyT1 inhibitor or a pharma- ceutically acceptable salt thereof, or a prodrug of a GlyT1 inhibitor or a pharma- ceutically acceptable salt thereof. In some embodiments, the present disclosure relates to a method of inhibiting ZPPIX synthesis in vivo by at least 10% (e.g., 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or at least 100%). In some embodiments, the present disclosure relates to a method of inhibiting ZPPIX synthesis in vivo by at least 20%. In some embodiments, the present disclosure relates to a method of inhibiting ZPPIX synthesis in vivo by at least 30%. In some embodiments, the present disclosure relates to a method of inhibiting ZPPIX synthesis in vivo by at least 40%. In some embodiments, the present disclosure relates to a method of inhibiting ZPPIX synthesis in vivo by at least 50%. In some embodiments, the disclosure relates to methods of inhibiting ZPPIX synthesis in vivo by at least 60%. In some embodiments, the disclosure relates to methods of inhibiting ZPPIX synthesis in vivo by at least 70%. In some embodiments, the disclosure relates to methods of inhibiting ZPPIX synthesis in vivo by at least 80%. In some embodiments, the disclosure relates to methods of inhibiting ZPPIX synthesis in vivo by at least 90%. In some embodiments, the disclosure relates to methods of inhibiting ZPPIX synthesis in vivo by at least 100%.

[0284] In certain aspects, the present disclosure relates to a method for treating erythropoietic protoporphyria (EPP), X-linked protoporphyria (XLPP) or congenital erythropoietic porphyria (CEP) in a subject, comprising administering to the subject a pharmaceutical composition comprising one or more glycine transporter inhibitors (e.g., GlyT1 inhibitors) or pharma- ceutically acceptable salts thereof, or one or more prodrugs of glycine transporter inhibitors (e.g., GlyT1 inhibitors) or salts thereof, wherein the subject has an increased 5-aminolevulinic acid (5-ALA) level. In some embodiments, the method relates to a subject having a 5-ALA level that is at least 10%, 20%, 30%, 40% or 50% higher than the 5-ALA level in a healthy subject before administration of a glycine transporter inhibitor (e.g., GlyT1 inhibitor). In some embodiments, the method relates to a subject having a 5-ALA level at least 10% higher than the 5-ALA level in a healthy subject before administration of a glycine transporter inhibitor (e.g., a GlyT1 inhibitor). In some embodiments, the method relates to a subject having a 5-ALA level at least 20% higher than the 5-ALA level in a healthy subject before administration of a glycine transporter inhibitor (e.g., a GlyT1 inhibitor). In some embodiments, the method relates to a subject having a 5-ALA level at least 30% higher than the 5-ALA level in a healthy subject before administration of a glycine transporter inhibitor (e.g., a GlyT1 inhibitor). In some embodiments, the method relates to a subject having a 5-ALA level at least 40% higher than the 5-ALA level in a healthy subject before administration of a glycine transporter inhibitor (e.g., a GlyT1 inhibitor). In some embodiments, the method relates to a subject having a 5-ALA level at least 50% higher than the 5-ALA level in a healthy subject before administration of a glycine transporter inhibitor (e.g., a GlyT1 inhibitor).

[0285] In certain aspects, the present disclosure relates to a method for inhibiting 5-aminolevulinic acid (5-ALA) synthesis in vivo, comprising administering a GlyT1 inhibitor or a pharma- ceutically acceptable salt thereof, or a prodrug of a GlyT1 inhibitor or a pharma- ceutically acceptable salt thereof to a subject. In some embodiments, the present disclosure relates to a method for inhibiting 5-ALA synthesis in vivo by at least 10% (e.g., 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or at least 100%). In some embodiments, the present disclosure relates to a method for inhibiting 5-ALA synthesis in vivo by at least 20%. In some embodiments, the present disclosure relates to a method for inhibiting 5-ALA synthesis in vivo by at least 30%. In some embodiments, the present disclosure relates to a method for inhibiting 5-ALA synthesis in vivo by at least 40%. In some embodiments, the present disclosure relates to a method for inhibiting 5-ALA synthesis in vivo by at least 50%. In some embodiments, the disclosure relates to methods of inhibiting 5-ALA synthesis in vivo by at least 60%. In some embodiments, the disclosure relates to methods of inhibiting 5-ALA synthesis in vivo by at least 70%. In some embodiments, the disclosure relates to methods of inhibiting 5-ALA synthesis in vivo by at least 80%. In some embodiments, the disclosure relates to methods of inhibiting 5-ALA synthesis in vivo by at least 90%. In some embodiments, the disclosure relates to methods of inhibiting 5-ALA synthesis in vivo by at least 100%.

[0286] The present application further provides the use of one or more glycine transporter inhibitors or their pharmaceutically acceptable salts, or one or more prodrugs of glycine transporter inhibitors or their pharmaceutically acceptable salts, in the manufacture of a formulation for treating EPP, XLPP, CEP, or their associated syndromes (e.g., EPP-related syndrome, XLPP-related syndrome, or CEP-related syndrome) in a subject. In some embodiments, the present application provides the use of one or more glycine transporter inhibitors or their pharmaceutically acceptable salts, or one or more prodrugs of glycine transporter inhibitors or their pharmaceutically acceptable salts, in the manufacture of a formulation for treating EPP, XLPP, or CEP in a subject. In some embodiments, the one or more glycine transporter inhibitors are one or more GlyT1 and / or GlyT2 inhibitors. In some embodiments, the one or more glycine transporter inhibitors are one or more GlyT1 inhibitors, for example, one or more GlyT1 inhibitors disclosed herein. In certain such embodiments, the GlyT1 inhibitor is bitopertin or a pharma- ceutically acceptable salt thereof, or a prodrug of bitopertin or a pharma- ceutically acceptable salt thereof. In certain of the foregoing embodiments, the formulation is administered in a therapeutically effective amount.

[0287] The present application provides the use of one or more glycine transporter inhibitors or their pharma- ceutically acceptable salts, or one or more prodrugs of glycine transporter inhibitors or their pharma- ceutically acceptable salts, in the manufacture of a pharmaceutical composition for treating EPP, XLPP, or CEP, or their associated syndromes (e.g., EPP-related syndrome, XLPP-related syndrome, or CEP-related syndrome) in a subject. In some embodiments, the present application provides the use of one or more glycine transporter inhibitors or their pharma- ceutically acceptable salts, or one or more prodrugs of glycine transporter inhibitors or their pharma- ceutically acceptable salts, in the manufacture of a pharmaceutical composition for treating EPP, XLPP, or CEP in a subject. In some embodiments, the one or more glycine transporter inhibitors are one or more GlyT1 and / or GlyT2 inhibitors. In some embodiments, the one or more glycine transporter inhibitors are one or more GlyT1 inhibitors, for example, one or more GlyT1 inhibitors disclosed herein. In certain such embodiments, the GlyT1 inhibitor is bitopertin or a pharma- ceutically acceptable salt thereof, or a prodrug of bitopertin or a pharma- ceutically acceptable salt thereof. In certain of the foregoing embodiments, the pharmaceutical composition further comprises a pharma- ceutically acceptable carrier.

[0288] Congenital erythropoietic porphyria (CEP) is an erythropoietic cutaneous porphyria characterized by bullous photosensitivity. Severe cases of CEP may present in utero with hydrops fetalis or shortly after birth with severe bullous photosensitivity, red urine, splenomegaly, hemolysis and transfusion dependency. Milder cases and later onset typically present with red urine, severe blistering and hemolytic anemia.

[0289] Individuals with CEP are often compound homozygous or heterozygous for UROS mutations. Some cases of CEP are due to mutations in the gene encoding the transcription factor GATA1. These mutations result in a reduction in the enzymatic activity of uroporphyrinogen III synthase (UROIII-S), the fourth enzyme in the heme biosynthetic pathway. The reduction in activity of UROIII-S results in the accumulation of hydroxymethylbilane, which spontaneously forms uroporphyrinogen I, which is further metabolized to coproporphyrinogen I. Uroporphyrinogen I and coproporphyrinogen I accumulate in tissues.

[0290] The diagnosis of CEP can be determined by analyzing the enzyme activity of uroporphyrinogen III synthase (UROIII-S), evaluating mutations in the UROS gene, evaluating the function of GATA-1 erythroid-specific transcription factor, evaluating mutations in GATA1, and determining the levels of uroporphyrin I and coproporphyrin I in the subject. In some embodiments, the subject has a mutation in UROS. In some embodiments, the subject has a genetic defect in GATA-1 erythroid-specific transcription factor. In some embodiments, the method relates to a method of treating a subject, wherein the subject has a decreased activity of uroporphyrinogen III synthase. In some embodiments, the increased levels of uroporphyrin I and / or coproporphyrin I are measured in the urine or red blood cells of the subject. In some embodiments, the increased levels of coproporphyrin I are measured in the feces of the subject.

[0291] In certain aspects, the present disclosure relates to a method of treating congenital erythropoietic porphyria (CEP) in a subject, comprising administering to the subject a pharmaceutical composition comprising one or more glycine transporter inhibitors (e.g., GlyT1 inhibitors) or pharma- ceutically acceptable salts thereof, or one or more prodrugs of glycine transporter inhibitors (e.g., GlyT1 inhibitors) or salts thereof, wherein the subject has increased uroporphyrin I and / or coproporphyrin I levels. In some embodiments, the subject has increased uroporphyrin I and / or coproporphyrin I levels. In some embodiments, the method relates to a subject having uroporphyrin I levels at least 10%, 20%, 30%, 40%, or 50% higher than the uroporphyrin I levels in healthy subjects prior to administration of a glycine transporter inhibitor (e.g., GlyT1 inhibitor). In some embodiments, the method relates to a subject having a uroporphyrin I level at least 10% higher than the uroporphyrin I level in a healthy subject before administration of a glycine transporter inhibitor (e.g., a GlyT1 inhibitor). In some embodiments, the method relates to a subject having a uroporphyrin I level at least 20% higher than the uroporphyrin I level in a healthy subject before administration of a glycine transporter inhibitor (e.g., a GlyT1 inhibitor). In some embodiments, the method relates to a subject having a uroporphyrin I level at least 30% higher than the uroporphyrin I level in a healthy subject before administration of a glycine transporter inhibitor (e.g., a GlyT1 inhibitor). In some embodiments, the method relates to a subject having a uroporphyrin I level at least 40% higher than the uroporphyrin I level in a healthy subject before administration of a glycine transporter inhibitor (e.g., a GlyT1 inhibitor). In some embodiments, the methods relate to a subject having a uroporphyrin I level that is at least 50% higher than the uroporphyrin I level in a healthy subject prior to administration of a glycine transporter inhibitor (eg, a GlyT1 inhibitor).

[0292] In some embodiments, the present disclosure relates to a method of treating a subject having a coproporphyrin I level at least 10%, 20%, 30%, 40% or 50% higher than the coproporphyrin I level in a healthy subject before administration of a glycine transporter inhibitor (e.g., a GlyT1 inhibitor). In some embodiments, the method relates to a subject having a coproporphyrin I level at least 10% higher than the coproporphyrin I level in a healthy subject before administration of a glycine transporter inhibitor (e.g., a GlyT1 inhibitor). In some embodiments, the method relates to a subject having a coproporphyrin I level at least 20% higher than the coproporphyrin I level in a healthy subject before administration of a glycine transporter inhibitor (e.g., a GlyT1 inhibitor). In some embodiments, the method relates to a subject having a coproporphyrin I level at least 30% higher than the coproporphyrin I level in a healthy subject before administration of a glycine transporter inhibitor (e.g., a GlyT1 inhibitor). In some embodiments, the method relates to a subject having a coproporphyrin I level at least 40% higher than the coproporphyrin I level in a healthy subject prior to administration of a glycine transporter inhibitor (e.g., a GlyT1 inhibitor). In some embodiments, the method relates to a subject having a coproporphyrin I level at least 50% higher than the coproporphyrin I level in a healthy subject prior to administration of a glycine transporter inhibitor (e.g., a GlyT1 inhibitor).

[0293] In certain aspects, the present disclosure relates to a method of inhibiting uroporphyrin I and / or coproporphyrin I synthesis in vivo, comprising administering to a subject a glycine transporter inhibitor (e.g., a GlyT1 inhibitor) or a pharma- ceutically acceptable salt thereof, or a prodrug of a glycine transporter inhibitor (e.g., a GlyT1 inhibitor) or a pharma- ceutically acceptable salt thereof. In some embodiments, the present disclosure relates to a method of inhibiting uroporphyrin I synthesis in vivo by at least 10% (e.g., 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or at least 100%). In some embodiments, the present disclosure relates to a method of inhibiting uroporphyrin I synthesis in vivo by at least 20%. In some embodiments, the present disclosure relates to a method of inhibiting uroporphyrin I synthesis in vivo by at least 30%. In some embodiments, the present disclosure relates to a method of inhibiting uroporphyrin I synthesis in vivo by at least 40%. In some embodiments, the present disclosure relates to methods of inhibiting uroporphyrin I synthesis in vivo by at least 50%. In some embodiments, the present disclosure relates to methods of inhibiting uroporphyrin I synthesis in vivo by at least 60%. In some embodiments, the present disclosure relates to methods of inhibiting uroporphyrin I synthesis in vivo by at least 70%. In some embodiments, the present disclosure relates to methods of inhibiting uroporphyrin I synthesis in vivo by at least 80%. In some embodiments, the present disclosure relates to methods of inhibiting uroporphyrin I synthesis in vivo by at least 90%. In some embodiments, the present disclosure relates to methods of inhibiting uroporphyrin I synthesis in vivo by at least 100%.

[0294] In some embodiments, the present disclosure relates to methods of inhibiting coproporphyrin I synthesis in vivo by at least 10% (e.g., 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or at least 100%). In some embodiments, the present disclosure relates to methods of inhibiting coproporphyrin I synthesis in vivo by at least 20%. In some embodiments, the present disclosure relates to methods of inhibiting coproporphyrin I synthesis in vivo by at least 30%. In some embodiments, the present disclosure relates to methods of inhibiting coproporphyrin I synthesis in vivo by at least 40%. In some embodiments, the present disclosure relates to methods of inhibiting coproporphyrin I synthesis in vivo by at least 50%. In some embodiments, the present disclosure relates to methods of inhibiting coproporphyrin I synthesis in vivo by at least 60%. In some embodiments, the present disclosure relates to methods of inhibiting coproporphyrin I synthesis in vivo by at least 70%. In some embodiments, the present disclosure relates to methods of inhibiting coproporphyrin I synthesis in vivo by at least 80%, hi some embodiments, the present disclosure relates to methods of inhibiting coproporphyrin I synthesis in vivo by at least 90%, hi some embodiments, the present disclosure relates to methods of inhibiting coproporphyrin I synthesis in vivo by at least 100%.

[0295] Porphyrins (e.g., PPIX, ZPPIX, uroporphyrin I, and coproporphyrin I) can be found in a variety of biological samples, including skin, urine, feces, plasma, and red blood cells. In some embodiments, porphyrins may be extracted from the biological sample into solution for fluorescence analysis. Porphyrins can be detected in these biological samples by direct examination using long wavelength ultraviolet light (e.g., 400-420 nm light). Porphyrins have maximum absorption wavelengths around 400-420 nm, with their highest absorption peak occurring at 415 nm. The emission maximum of porphyrins is typically around 600 nm, varying slightly based on the type of porphyrin and solvent used for analysis. In some embodiments, diagnosis of EPP, XLPP, and CEP may be performed using fluorescence analysis. In some embodiments, skin porphyrin levels (e.g., PPIX levels) can be measured by calculating the difference before and after complete photobleaching of PPIX using controlled illumination. See, e.g., Heerfordt IM. Br J Dermatol. 2016;175(6):1284-1289.

[0296] In some embodiments, the subject's plasma porphyrins fluoresce with a peak of 634 nm when illuminated with blue light (e.g., 400-420 nm light). In some embodiments, the subject's plasma porphyrins fluoresce with a peak of 626 nm to 634 nm when illuminated with blue light (e.g., 400-420 nm light). In some embodiments, the subject's skin porphyrins fluoresce with a peak of 632 nm when illuminated with blue light (e.g., 400-420 nm light). In some embodiments, the subject's skin porphyrins fluoresce with a peak of 626 nm to 634 nm when illuminated with blue light (e.g., 400-420 nm light). In some embodiments, the subject has a protoporphyrin IX level in the skin that is greater than 0.2 FluoDerm Units (FDU). In some embodiments, the subject has a protoporphyrin IX level in the skin that is greater than 1.0 FDU. In some embodiments, the subject has a protoporphyrin IX level in the skin between 1.0 FDU and 2.5 FDU. In some embodiments, the subject has a protoporphyrin IX level in the skin greater than 2.5 FDU. In some embodiments, the method reduces the protoporphyrin IX level in the skin of the subject to less than 0.5 FDU. In some embodiments, the method reduces the protoporphyrin IX level in the skin of the subject to less than 1.0 FDU. In some embodiments, the method reduces the protoporphyrin IX level in the skin of the subject to less than 1.5 FDU. In some embodiments, the method reduces the protoporphyrin IX level in the skin of the subject to less than 2.0 FDU. In some embodiments, the method reduces the protoporphyrin IX level in the skin of the subject to less than 2.5 FDU. In some embodiments, the subject has red fluorescent urine. In some embodiments, the subject has a peak at 615 nm to 620 nm using plasma porphyrin fluorescence analysis.

[0297] In certain aspects, the disclosure relates to a method of preventing, treating, or reducing the rate of progression and / or severity of one or more complications of EPP, XLPP, or CEP in a subject, comprising administering to the subject a pharmaceutical composition comprising one or more glycine transporter inhibitors (e.g., GlyT1 inhibitors) or pharma- ceutically acceptable salts thereof, or prodrugs of one or more glycine transporter inhibitors (e.g., GlyT1 inhibitors) or salts thereof. In some embodiments, the one or more complications of EPP, XLPP or CEP are selected from the group consisting of acute photosensitivity, cutaneous photosensitivity, edema, erythema, anemia, hypochromic anemia, hemolytic anemia, hemolysis, mild hemolysis, severe hemolysis, chronic hemolysis, hypersplenism, palmar keratoderma, blisters, lesions, scarring, deformity, loss of nails, loss of digits, cholelithiasis, cholestasis, cytolysis, gallstones, cholestatic liver failure, red teeth, hypercellular bone marrow, myelodysplasia, thrombocytopenia, fetal hydrops, and / or intrauterine death. In some embodiments, the present disclosure contemplates a method of treating one or more complications of EPP, XLPP or CEP (e.g., acute photosensitivity, cutaneous photosensitivity, edema, erythema, anemia, hypochromic anemia, hemolytic anemia, hemolysis, mild hemolysis, severe hemolysis, chronic hemolysis, hypersplenism, palmar keratoderma, blisters, lesions, scarring, deformity, loss of nails, loss of digits, cholelithiasis, cholestasis, cell lysis, gallstones, cholestatic liver failure, red teeth, hypercellular bone marrow, myelodysplasia, thrombocytopenia, fetal hydrops and / or intrauterine death) comprising administering to a subject a pharmaceutical composition comprising one or more glycine transporter inhibitors (e.g., GlyT1 inhibitors) or a pharma- ceutically acceptable salt thereof, or a prodrug or salt thereof of one or more glycine transporter inhibitors (e.g., GlyT1 inhibitors). In some embodiments, the one or more complications are ameliorated indirectly.In some embodiments, the present disclosure contemplates a method of preventing one or more complications of EPP, XLPP, or CEP, comprising administering to a subject a pharmaceutical composition comprising one or more glycine transporter inhibitors (e.g., GlyT1 inhibitors) or pharma- ceutically acceptable salts thereof, or one or more prodrugs or salts thereof of glycine transporter inhibitors (e.g., GlyT1 inhibitors). In some embodiments, the present disclosure contemplates a method of reducing the rate of progression of one or more complications of EPP, XLPP, or CEP, comprising administering to a subject a pharmaceutical composition comprising one or more glycine transporter inhibitors (e.g., GlyT1 inhibitors) or pharma- ceutically acceptable salts thereof, or one or more prodrugs or salts thereof of glycine transporter inhibitors (e.g., GlyT1 inhibitors). In some embodiments, the disclosure contemplates a method of reducing the severity of one or more complications of EPP, XLPP, or CEP, comprising administering to a subject a pharmaceutical composition comprising one or more glycine transporter inhibitors (e.g., GlyT1 inhibitors) or pharma- ceutically acceptable salts thereof, or prodrugs of one or more glycine transporter inhibitors (e.g., GlyT1 inhibitors) or salts thereof.

[0298] Optionally, the methods disclosed herein for preventing or treating one or more complications of EPP, XLPP or CEP in a subject, or reducing the rate of progression and / or severity thereof, may further comprise administering to the patient one or more supportive therapies or additional active agents for treating EPP, XLPP or CEP. For example, the patient may also be administered one or more supportive therapies or active agents selected from the group consisting of sun avoidance, topical sunscreens, skin protection, UVB phototherapy, afamelanotide (Scenesse®), bortezomib, proteasome inhibitors, chemical chaperones, cholestyramine, activated charcoal, iron supplementation, liver transplantation, bone marrow transplantation, splenectomy and blood transfusion. In some embodiments, the methods described herein may further comprise administering to the patient afamelanotide (Scenesse®).

[0299] Porphyrin photosensitivity in EPP, XLPP and CEP produces two distinct clinical syndromes: (1) acute photosensitivity to sun exposure accompanied by erythema and edema, and (2) subepidermal blisters appear in sun-exposed areas of skin.In certain aspects, the present disclosure relates to a method for preventing, treating, or reducing the progression rate and / or severity of EPP, XLPP or CEP in a subject, comprising administering to the subject a pharmaceutical composition comprising one or more glycine transporter inhibitors (e.g., GlyT1 inhibitors) or pharma- ceutical acceptable salts thereof, or one or more glycine transporter inhibitors (e.g., GlyT1 inhibitors) prodrugs or salts thereof, wherein the method increases painless light exposure in the subject.In some embodiments, the method increases painless light exposure in the subject by at least 10%, 20%, 30%, 40% or 50% higher than painless light exposure before administration of the GlyT1 inhibitor.In some embodiments, the method reduces photosensitivity in the subject. In some embodiments, the method reduces the photosensitivity in the subject by at least 10%, 20%, 30%, 40% or 50% compared to the photosensitivity before administration of the GlyT1 inhibitor. In some embodiments, the subject has a history of phototoxicity reaction from EPP. In some embodiments, the subject is an adult, a child, an infant, or a pregnant woman.

[0300] Glycine is one of the important starting materials for heme and globin synthesis. Therefore, the reduction in the level of glycine due to GlyT1 inhibition can result in a reduction in heme synthesis. In certain aspects, the present disclosure relates to a method of treating EPP, XLPP, or CEP in a subject, comprising administering to the subject a pharmaceutical composition comprising one or more glycine transporter inhibitors (e.g., GlyT1 inhibitors) or pharma- ceutically acceptable salts thereof, or one or more prodrugs of glycine transporter inhibitors (e.g., GlyT1 inhibitors) or salts thereof, wherein the heme level of the subject is not reduced by more than 10% (e.g., 10%, 15%, 20%, 25%, and 30%). In some embodiments, the present disclosure relates to a method of treating EPP, XLPP, or CEP in a subject, wherein the heme level of the subject is not reduced by more than 15%. In some embodiments, the present disclosure relates to a method of treating EPP, XLPP, or CEP in a subject, wherein the heme level of the subject is not reduced by more than 20%. In some embodiments, the present disclosure relates to a method of treating EPP, XLPP or CEP in a subject, wherein the heme levels in the subject are not decreased by more than 25%. In some embodiments, the present disclosure relates to a method of treating EPP, XLPP or CEP in a subject, wherein the heme levels in the subject are not decreased by more than 30%.

[0301] In certain aspects, the present disclosure relates to a method of treating EPP, XLPP or CEP in a subject, comprising administering to the subject a pharmaceutical composition comprising one or more glycine transporter inhibitors (e.g., GlyT1 inhibitors) or pharma- ceutically acceptable salts thereof, or one or more prodrugs of glycine transporter inhibitors (e.g., GlyT1 inhibitors) or salts thereof, wherein the subject's PPIX level is reduced while the patient's heme level is substantially maintained. In some embodiments, the patient's PPIX level is reduced by at least 50% (e.g., 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or at least 100%) and the patient's heme level is not reduced by more than 10% (e.g., 10%, 15%, 20%, 25% and 30%). In some embodiments, the patient's PPIX level is reduced by at least 85% and the patient's heme level is not reduced by more than 15%. In some embodiments, the patient's PPIX level is reduced by at least 80% and the patient's heme level is not reduced by more than 15%. In some embodiments, the patient's PPIX level is reduced by at least 75% and the patient's heme level is not reduced by more than 15%. In some embodiments, the patient's PPIX level is reduced by at least 70% and the patient's heme level is not reduced by more than 15%. In some embodiments, the patient's PPIX level is reduced by at least 65% and the patient's heme level is not reduced by more than 15%. In some embodiments, the patient's PPIX level is reduced by at least 60% and the patient's heme level is not reduced by more than 15%. In some embodiments, the patient's PPIX level is reduced by at least 55% and the patient's heme level is not reduced by more than 15%. In some embodiments, the patient's PPIX level is reduced by at least 50% and the patient's heme level is not reduced by more than 15%.

[0302] In certain aspects, the disclosure relates to a method of treating EPP, XLPP or CEP in a subject, comprising administering to the subject a pharmaceutical composition comprising one or more glycine transporter inhibitors (e.g., GlyT1 inhibitors) or pharma- ceutically acceptable salts thereof, or one or more prodrugs of glycine transporter inhibitors (e.g., GlyT1 inhibitors) or salts thereof, wherein administering the pharmaceutical composition does not cause a substantial reduction in heme levels. In some embodiments, the patient's PPIX level is reduced by at least 50% (e.g., 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or at least 100%). In some embodiments, the patient's PPIX level is reduced by at least 55%. In some embodiments, the patient's PPIX level is reduced by at least 60%. In some embodiments, the patient's PPIX level is reduced by at least 65%. In some embodiments, the patient's PPIX level is reduced by at least 70%. In some embodiments, the patient's PPIX level is reduced by at least 75%. In some embodiments, the patient's PPIX levels are decreased by at least 80%. In some embodiments, the patient's PPIX levels are decreased by at least 85%. In some embodiments, the patient's PPIX levels are decreased by at least 90%. In some embodiments, the patient's PPIX levels are decreased by at least 95%. In some embodiments, the patient's PPIX levels are decreased by at least 100%. In some embodiments, the patient's heme levels are not decreased by more than 10% (e.g., 10%, 15%, 20%, 25% and 30%). In some embodiments, the patient's heme levels are not decreased by more than 15%. In some embodiments, the patient's heme levels are not decreased by more than 20%. In some embodiments, the patient's heme levels are not decreased by more than 25%. In some embodiments, the patient's heme levels are not decreased by more than 30%.

[0303] In some embodiments, accumulation of one or more subsequent heme intermediates is inhibited, where the one or more heme intermediates are selected from the group consisting of PPIX, ZPPIX, uroporphyrin I, coproporphyrin I, and / or 5-ALA. In some embodiments, the disclosure relates to a method of inhibiting accumulation of PPIX, comprising administering to a subject a pharmaceutical composition comprising one or more glycine transporter inhibitors (e.g., GlyT1 inhibitors) or pharma- ceutically acceptable salts thereof, or a prodrug or salt of one or more glycine transporter inhibitors (e.g., GlyT1 inhibitors). In some embodiments, the disclosure relates to a method of inhibiting accumulation of ZPPIX, comprising administering to a subject a pharmaceutical composition comprising one or more glycine transporter inhibitors (e.g., GlyT1 inhibitors) or pharma- ceutically acceptable salts thereof, or a prodrug or salt of one or more glycine transporter inhibitors (e.g., GlyT1 inhibitors). In some embodiments, the disclosure relates to a method of inhibiting accumulation of uroporphyrin I, comprising administering to a subject a pharmaceutical composition comprising one or more glycine transporter inhibitors (e.g., GlyT1 inhibitors) or pharma- ceutically acceptable salts thereof, or a prodrug or salt thereof of one or more glycine transporter inhibitors (e.g., GlyT1 inhibitors). In some embodiments, the disclosure relates to a method of inhibiting accumulation of coproporphyrin I, comprising administering to a subject a pharmaceutical composition comprising one or more glycine transporter inhibitors (e.g., GlyT1 inhibitors) or pharma- ceutically acceptable salts thereof, or a prodrug or salt thereof of one or more glycine transporter inhibitors (e.g., GlyT1 inhibitors). In some embodiments, the disclosure relates to a method of inhibiting accumulation of 5-ALA comprising administering to a subject a pharmaceutical composition comprising one or more glycine transporter inhibitors (e.g., GlyT1 inhibitors) or pharma- ceutically acceptable salts thereof, or a prodrug of one or more glycine transporter inhibitors (e.g., GlyT1 inhibitors) or salts thereof.In some embodiments, accumulation of one or more heme intermediates (e.g., PPIX, ZPPIX, uroporphyrin I, coproporphyrin I and / or 5-ALA) is inhibited in a dose-dependent manner. See, e.g., FIG. 7.

[0304] Protoporphyrin accumulation in EPP, XLPP and CEP can cause liver injury if liver load exceeds bile canalicular drainage capacity. Accumulation of PPIX in hepatocytes and bile canaliculi can result in cell damage, cholestasis, cell lysis, and further retention of protoporphyrin. Excess protoporphyrin can have a cholestatic effect resulting in hepatobiliary changes that can range from mild inflammation to fibrosis and cirrhosis (e.g., cholelithiasis, mild liver disease, worsening liver disease and end-stage liver disease). 3-5% of patients with EPP or XLPP develop protoporphyric liver disease, a rapidly progressing severe liver disease that may require liver transplantation. Approximately 2% of patients develop severe liver disease.

[0305] In certain aspects, the present disclosure relates to a method for preventing, treating, or reducing the progression rate and / or severity of liver disease associated with EPP, XLPP, or CEP in a subject, comprising administering to the subject a pharmaceutical composition comprising one or more glycine transporter inhibitors (e.g., GlyT1 inhibitors) or their pharma- ceutically acceptable salts, or one or more prodrugs of glycine transporter inhibitors (e.g., GlyT1 inhibitors) or their salts. In some embodiments, the liver disease associated with EPP, XLPP, or CEP is cholelithiasis. In some embodiments, the liver disease associated with EPP, XLPP, or CEP is mild liver disease. In some embodiments, the liver disease associated with EPP, XLPP, or CEP is exacerbation of liver disease. In some embodiments, the liver disease associated with EPP, XLPP, or CEP is end-stage liver disease.

[0306] Liver function in patients with EPP, XLPP and CEP can be assessed using various known clinical assays. In some embodiments, liver function tests can be used to determine the levels of various biochemical parameters (e.g., elevated aspartate transaminase levels, alkaline phosphatase or gamma-glutamyltransferase levels). In some embodiments, histopathology of liver biopsy can be used to assess one or more parameters in a subject (e.g., protoporphyrin deposition, fibrosis, infiltration, portal fibrosis and periportal fibrosis). In some embodiments, ultrastructural studies of biopsy specimens can be used to determine whether crystals containing vacuoles are present in a subject. Deterioration of liver function increases urinary coproporphyrin excretion. In some embodiments, urinary coproporphyrin excretion can be analyzed to assess liver function in a subject. In some embodiments, ultrasound or magnetic resonance elastography can be used to measure liver stiffness in a subject.

[0307] In certain embodiments of the methods and uses disclosed herein, the glycine transporter inhibitor, e.g., a GlyT1 inhibitor (e.g., a GlyT1 inhibitor disclosed herein) or a pharma- ceutically acceptable salt thereof, or a prodrug of a glycine transporter inhibitor, e.g., a GlyT1 inhibitor (e.g., a GlyT1 inhibitor disclosed herein) or a pharma- ceutically acceptable salt thereof, demonstrates PPIX inhibition with an EC50 of less than 500 nM, less than 400 nM, less than 300 nM, less than 200 nM, or less than 100 nM. In certain embodiments of the present application, the glycine transporter inhibitor, e.g., a GlyT1 inhibitor (e.g., a GlyT1 inhibitor disclosed herein) or a pharma- ceutically acceptable salt thereof, or a prodrug of a glycine transporter inhibitor, e.g., a GlyT1 inhibitor (e.g., a GlyT1 inhibitor disclosed herein) or a pharma- ceutically acceptable salt thereof, demonstrates PPIX inhibition with an EC50 of less than 100 nM. In certain embodiments of the present application, the glycine transporter inhibitor, for example, a GlyT1 inhibitor (e.g., a GlyT1 inhibitor disclosed herein) or a pharma- ceutically acceptable salt thereof, or a prodrug of a glycine transporter inhibitor, for example, a GlyT1 inhibitor (e.g., a GlyT1 inhibitor disclosed herein) or a pharma- ceutically acceptable salt thereof, demonstrates PPIX inhibition with an EC50 of less than 50 nM. In certain such embodiments, the EC50 is measured in a flow cytometry assay. In certain of the foregoing embodiments, the GlyT1 inhibitor is bitopertin or a pharma- ceutically acceptable salt thereof, or a prodrug of bitopertin or a pharma- ceutically acceptable salt thereof.

[0308] In certain embodiments of the methods and uses disclosed herein, at least 50%, at least 60%, at least 70%, at least 80%, at least 85%, at least 90%, or at least 95% cell viability is maintained. In certain such embodiments, at least 90% cell viability is maintained. The present disclosure also provides the following non-limiting embodiments.

[0309] In order to allow the embodiments disclosed herein to be more effectively understood, examples are provided below. It should be understood that these examples are for illustrative purposes only and are not to be construed as limiting the embodiments in any way. Throughout these examples, there may be molecular cloning reactions and other standard recombinant DNA techniques described, which, unless otherwise stated, were carried out according to the method described in Maniatis et al., Molecular Cloning - A Laboratory Manual, 2nd ed., Cold Spring Harbor Press (1989), using commercially available reagents.

[0310] The following examples are illustrative rather than limiting of the methods and compositions described herein. Other suitable modifications and adaptations of the variety of conditions and parameters normally encountered in the therapeutic, synthetic and other embodiments disclosed herein are within the spirit and scope of the embodiments. EXAMPLES

[0311] Example 1 Compound synthesis The compounds disclosed herein can be prepared by the process known and disclosed in the art according to well-known procedures.For example, the compound of formula I, such as Bitopertin, can be prepared according to the synthesis protocol provided in U.S. Patent No. 7,319,099, U.S. Patent No. 9,877,963 and U.S. Patent No. 7,812,161, the contents of which are incorporated herein by reference in their entirety.In addition, the compound of formula II, such as PF-3463275, can be prepared according to the synthesis protocol provided in U.S. Patent No. 8,124,639, the contents of which are incorporated herein by reference in their entirety. Example 2 GlyT1 Inhibitors for Treating Subjects with Erythropoietic Protoporphyria (EPP), X-Linked Protoporphyria (XLPP) and Congenital Erythropoietic Porphyria (CEP) (Prophetic Examples)

[0312] Synthesis of large amounts of heme is a fundamental requirement in developing red blood cells to support the production of large amounts of hemoglobin. In this cell lineage, the amount of heme required to meet this demand is disproportionately greater than any other cell type. Heme synthesis begins with the condensation of glycine with succinyl-CoA by the enzyme ALAS. This is the rate-limiting step in heme biosynthesis, ensuring that heme intermediates do not accumulate and cause toxicity. To meet this high demand for heme, red blood cells acquire an erythroid-specific form of ALAS (ALAS2) and the glycine transporter GlyT1 to increase the availability of glycine.

[0313] Animal and human studies in which GlyT1 activity was eliminated by genetic deletion (Garcia-Santos et al., 2017) or reduced by administration of specific GlyT1 inhibitors (Pinard et al., 2018) demonstrated that heme synthesis in red blood cells was reduced and impaired. These results indicate that modulation of glycine uptake in red cells can regulate the heme biosynthetic pathway.

[0314] In patients with either erythropoietic protoporphyria or congenital erythropoietic porphyria, specific mutations in individual genes encoding enzymes in the heme biosynthetic pathway result in altered enzyme activity and accumulation of heme intermediates upstream of the affected enzyme. Accumulation of these metabolites occurs because the mutated enzyme becomes a rate-limiting step in the pathway with insufficient activity to completely convert the upstream metabolite to the next step in the pathway. Three diseases are of specific interest. 1. EPP, caused by a mutation in the ferrochetalase gene, which leads to a reduction in the activity of this enzyme and the accumulation of the upstream metabolite protoporphyrin IX (PPIX). Rarely, EPP can be observed in an acquired form in older humans who develop new clones containing ferrochetalase mutations as a hallmark of myelodysplasia. 2. XLPP, caused by an activating mutation in the ALAS2 gene, which leads to high levels of PPIX. In this case, the accumulation of metabolites is downstream of the affected enzyme, due to overproduction that cannot be completely converted to heme even by normal levels of ferrochetalase. 3. CEP, caused by mutations in the gene for uroporphyrinogen synthase, leading to reduced activity of this enzyme and accumulation of the upstream metabolite coproporphyrin I.

[0315] These heme intermediates can escape red cells either by cellular hemolysis (in CEP) or by active transport out of the cell (in EPP and XLPP) and cause toxicity. A consistent feature of all three diseases is a severe, painful, blistering skin reaction following exposure to sunlight that causes persistent scarring and disfigurement. This is caused by local production of reactive intermediates by the action of sunlight on PPIX or coproporphyrin I, which leads to a severe inflammatory reaction. PPIX is hydrophobic and is therefore excreted through the bile duct. High bile concentrations can result in cholelithiasis, cholestasis and liver damage that can be severe, resulting in liver failure. In the case of CEP, accumulation of coproporphyrin in mature red cells can result in severe hemolytic anemia.

[0316] These disease manifestations of EPP, XLPP and CEP are caused by the overproduction of intermediate heme metabolites due to genetic abnormalities in the heme biosynthesis pathway. The accumulated metabolites are either toxic to red cells after accumulation in the skin and exposure to sunlight or due to bile excretion by the liver. GlyT1 controls the availability of one of the first substances in the heme biosynthesis pathway and has been shown to downregulate heme production in humans or animals with normal heme pathways described above. Without being bound by any particular theory, it is possible that GlyT1 reduces the production of intermediate metabolites of heme in the same way, especially when the intermediate products accumulate as a result of abnormal enzyme activity. Thus, subjects with EPP, XLPP or CEP are treated with GlyT1, which reduces the production of toxic metabolites in red blood cells in such subjects, resulting in reduced skin accumulation of these metabolites, reduced hepatic bile excretion, or in the case of CEP, reduced hemolysis, and in all cases, reduced severity of the disease. In this way, the disease is treated.

[0317] Example 3 MetGlyT1 inhibitors are effective in reducing levels of heme metabolites in erythroleukemia cell lines containing disease-causing mutations for EPP, XLPP, or CEP

[0318] Erythroleukemia cells are genetically modified to obtain cell lines that contain the mutations that cause disease for EPP, XLPP or CEP.These genetically modified cell lines are treated with GlyT1 inhibitor, and the production of heme metabolites is evaluated photometrically, biochemically, or in radiolabeling studies.The level of photohemolysis caused by PPIX is evaluated in these cell lines, and is found to be reduced in the presence of GlyT1 inhibitor. Example 4 GlyT1 inhibitors are effective in reducing the levels of heme metabolites in red blood cells containing disease-causing mutations for EPP, XLPP or CEP (Prophetic Examples)

[0319] Erythroid cells are taken from bone marrow or peripheral blood of animals with disease causing mutations in specific genes that cause EPP, XLPP or CEP. These cell lines are treated with GlyT1 inhibitors, and the production of heme metabolites is evaluated photometrically, biochemically, or in radiolabeling studies. The level of photohemolysis caused by PPIX is evaluated in these cell lines and found to be reduced in the presence of GlyT1 inhibitors. Example 5 GlyT1 inhibitors are effective in reducing the levels of heme metabolites in red blood cells of patients containing disease-causing mutations for EPP, XLPP or CEP (Prophetic Examples)

[0320] Erythroid cells (reticulocytes and erythrocytes) are obtained from patients with EPP, XLPP and CEP (when available). These cells from patients are treated with GlyT1 inhibitors, and the production of heme metabolites is evaluated photometrically, biochemically, or in radiolabel studies. The level of photohemolysis caused by PPIX is evaluated in these cell lines and found to be reduced in the presence of GlyT1 inhibitors. Example 6 GlyT1 inhibitors are effective in reducing the severity of EPP or XLPP in animals (Prophetic Examples)

[0321] Animals with EPP and XLPP are treated with one or more GlyT1 inhibitors at various doses over a period of time.The levels of toxic heme intermediates in such animals are found to be reduced, and symptoms of such diseases, such as the severity of skin reactions, hepatobiliary disease and / or hemolysis, are found to be ameliorated.

[0322] The embodiments and examples provided herein demonstrate that GlyT1 inhibitors can be used to treat EPP, XLPP or CEP, which is a surprising and unexpected result. Example 7 EPP cell model

[0323] A knockout guide sequence was designed to target exon 3 of the ferrochelatase gene. The guide sequences tested are shown in Table 1. [Table 1]

[0324] K562 cells were cultured in Iscove's Modified Dulbecco's Medium (IMDM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin / streptomycin (PS). CRISPR Cas9 RNP complexes with guide RNA were electroporated into K562 cells. Genomic DNA from pooled cells was isolated, amplified by PCR, and sequenced by Sanger sequencing to determine knockout efficiency. Single-cell clones were isolated by fluorescence-assisted cell sorting (FACS). TA cloning and Sanger sequencing were used to confirm single-cell clones and genotypes. Five clones were selected for further characterization by Western blot (clone IDs are Clone 1-7; Clone 1-9; Clone 1-10, Clone 1-32; Clone 1-51; and K562 WT) (Figure 1), to determine FECH protein expression levels (Antibody: FECH Antibody Rabbit Polyclonal, Proteintech, 14466-1-AP) and PPIX levels by flow cytometry (Figure 2). LC / MS / MS confirmed the accumulation of PPIX in Clone 1-9 compared to WT K562 cells (Figure 3). The genotypic characteristics of the five clones are presented in Table 2. [Table 2]

[0325] 2 x 10 in IMDM medium with 10% FBS and 1% PS 5900 μL of K562 clone 9 cells at 100 cells / mL were plated in a 24-well plate. After 24 hours of incubation, 100 μL of compounds in DMSO / medium were added at different concentrations. The final concentration of DMSO was 0.1%. Compounds were incubated at 37°C for 96 hours. Cell viability and cell counts were measured by a Vi-CELL XR complete system. Finally, the effect of compounds on PPIX levels was determined by flow cytometry. Figure 4 shows that both bitopertin and PF-03463275 demonstrated a dose-dependent inhibition of PPIX accumulation by flow cytometry up to 50%. Bitopertin showed an EC50 of 7 nM and PF-03463275 showed an EC50 of 46 nM. Figure 5 shows that both bitopertin and PF-03463275 had no negative effect on cell viability. Importantly, the LC / MS / MS method demonstrated that bitopertin reduced 5-aminolevulinic acid (5-ALA) and PPIX levels in the EPP K562 cell model with minimal effects on heme formation (Figures 6, 7 and 8).

[0326] Additional GlyT1 inhibitors also showed dose-dependent inhibition of PPIX accumulation, whereas the GlyT2 inhibitor, ORG-25543, [ka] did not show any inhibition up to the highest tested concentration of 10 μM (Table 3). [Table 3]

[0327] Example 8 GlyT1 inhibitors are effective in reducing PPIX levels in human hematopoietic stem cells transduced with lentivirus expressing FECH small interfering RNA (shRNA)

[0328] To investigate the effect of GlyT1 inhibitors in human hematopoietic stem cells with EPP phenotype, lentiviral vectors expressing shRNA of FECH were constructed (Table 4) and transduced at 25 MOI into human umbilical cord blood CD34+ cells purchased from Stemexpress. [Table 4]

[0329] RT-qPCR of the resulting CD34+ cells shows a 60% reduction in FECH mRNA levels compared to cells treated with a control lentiviral vector (Figure 9). Transduced CD34+ cells were differentiated into erythroid cells for 9 days in StemSpan SFEM II medium supplemented with StemSpan Erythroid Expansion Supplement in the presence of bitopertin (100 nM) or DMSO control. Erythroid cell antigen profile was analyzed using a cytofluorimetric strategy with the following surface markers: CD71 (PE mouse anti-human CD71, BD Biosciences), glycophorin A (APC mouse anti-human CD235a, BD Biosciences). After 9 days in differentiation culture, cell viability was higher than 60% in all samples and more than 80% of cells transduced with lentivirus expressing shRNA of FECH showed an increase in PPIX as determined by flow cytometry (Figure 10). Bitopertin (100 nM) treatment had no negative effect on erythroid cell surface markers and reduced PPIX accumulation by 60% (FIG. 11).

[0330] While preferred embodiments of the present application have been shown and described herein, it will be obvious to those skilled in the art that such embodiments are provided by way of example only. Numerous variations, changes and substitutions will occur to those skilled in the art without departing from the present application. It should be understood that various modifications to the embodiments of the application described herein may be used in the practice of the present application. It is intended that the following claims define the scope of the present application, and that methods and structures within the scope of these claims and their equivalents be covered therein. Incorporation by Reference

[0331] All references cited in this application and their references, where appropriate for teaching additional or alternative details, features and / or technical background, are hereby incorporated by reference in their entirety. The present invention provides, for example, the following items. (Item 1) 1. A method of treating erythropoietic protoporphyria (EPP), X-linked protoporphyria (XLPP), or congenital erythropoietic porphyria (CEP) in a subject, the method comprising administering to the subject a pharmaceutical composition comprising one or more glycine transporter 1 (GlyT1) inhibitors or pharma- ceutical acceptable salts thereof, or a prodrug of the one or more GlyT1 inhibitors or salts thereof. (Item 2) A method for preventing, treating, or reducing the rate of progression and / or severity of one or more complications of EPP, XLPP or CEP in a subject, said method comprising administering to the subject a pharmaceutical composition comprising one or more GlyT1 inhibitors or pharma- ceutical acceptable salts thereof, or a prodrug of said one or more GlyT1 inhibitors or pharma- ceutical acceptable salts thereof. (Item 3) 3. The method according to item 2, wherein the one or more complications of EPP, XLPP or CEP are selected from the group consisting of acute photosensitivity, cutaneous photosensitivity, edema, erythema, anemia, hypochromic anemia, hemolytic anemia, hemolysis, mild hemolysis, severe hemolysis, chronic hemolysis, hypersplenism, palmar keratoderma, blisters, lesions, scarring, deformity, loss of nails, loss of digits, cholestasis, cytolysis, gallstones, cholestatic liver failure, cholelithiasis, mild liver disease, worsening liver disease, end stage liver disease, red teeth, hypercellular bone marrow, myelodysplasia, thrombocytopenia, fetal hydrops and / or intrauterine death. (Item 4) A method for use in the prevention or treatment of EPP, XLPP or CEP in a subject, said use comprising administering to the subject a GlyT1 inhibitor or a pharma- ceutically acceptable salt thereof, or a prodrug of said one or more GlyT1 inhibitors or a pharma- ceutically acceptable salt thereof. (Item 5) A method for use in the manufacture of a medicament for the treatment of EPP, XLPP or CEP in a subject, said use comprising administering to the subject at least one GlyT1 inhibitor or a pharma- ceutically acceptable salt thereof, or one or more prodrugs of a GlyT1 inhibitor or a pharma- ceutically acceptable salt thereof. (Item 6) A method for use in the manufacture of a medicament for inhibiting protoporphyrin IX (PPIX) synthesis in vivo, said use comprising administering to a subject a GlyT1 inhibitor or a pharma- ceutically acceptable salt thereof, or one or more prodrugs of a GlyT1 inhibitor or a pharma- ceutically acceptable salt thereof. (Item 7) 7. The method according to any one of items 1 to 6, wherein the subject has EPP. (Item 8) 7. The method according to any one of items 1 to 6, wherein the subject has XLPP. (Item 9) 7. The method according to any one of items 1 to 6, wherein the subject has CEP. (Item 10) 4. The method of claim 3, wherein the acute photosensitivity is caused by exposure to sunlight. (Item 11) 11. The method of any one of items 1 to 10, wherein the method increases painless light exposure in the subject. (Item 12) 11. The method according to any one of items 1 to 10, wherein the method reduces photosensitivity in the subject. (Item 13) A method for inhibiting PPIX synthesis in vivo, comprising administering to a subject a GlyT1 inhibitor or a pharma- ceutically acceptable salt thereof, or a prodrug of the GlyT1 inhibitor or a pharma- ceutically acceptable salt thereof. (Item 14) A method for inhibiting zinc protoporphyrin IX (ZPPIX) synthesis in vivo, comprising administering to a subject a GlyT1 inhibitor or a pharma- ceutically acceptable salt thereof, or a prodrug of the GlyT1 inhibitor or a pharma- ceutically acceptable salt thereof. (Item 15) A method for inhibiting uroporphyrin I and / or coproporphyrin I synthesis in vivo, comprising administering to a subject a GlyT1 inhibitor or a pharma- ceutically acceptable salt thereof, or a prodrug of the GlyT1 inhibitor or a pharma- ceutically acceptable salt thereof. (Item 16) A method for inhibiting 5-aminolevulinic acid (5-ALA) synthesis in vivo, comprising administering to a subject a GlyT1 inhibitor or a pharma- ceutically acceptable salt thereof, or a prodrug of the GlyT1 inhibitor or a pharma- ceutically acceptable salt thereof. (Item 17) 17. The method according to any one of items 1 to 16, wherein accumulation of one or more heme intermediates is inhibited, said one or more heme intermediates being selected from the group consisting of PPIX, ZPPIX, uroporphyrin I, coproporphyrin I and / or 5-ALA. (Item 18) 18. The method of claim 17, wherein the accumulation of the one or more heme intermediates is inhibited in a dose-dependent manner. (Item 19) The method of any one of the preceding items, wherein the GlyT1 inhibitor demonstrates an EC50 of less than 500 nM. (Item 20) The method of any one of the preceding items, wherein the GlyT1 inhibitor demonstrates an EC50 of less than 100 nM. (Item 21) 8. The method of any one of the preceding items, wherein at least 50% cell viability is maintained. (Item 22) 8. The method of any one of the preceding items, wherein at least 90% cell viability is maintained. (Item 23) 23. The method according to any one of items 1 to 22, wherein the subject has a PPIX level that is at least 10%, 20%, 30%, 40% or 50% higher than the PPIX level in a healthy subject prior to administration of the GlyT1 inhibitor. (Item 24) 23. The method of any one of items 1 to 22, wherein the subject has a ZPPIX level that is at least 10%, 20%, 30%, 40% or 50% higher than the ZPPIX level in a healthy subject prior to administration of the GlyT1 inhibitor. (Item 25) 23. The method according to any one of items 1 to 22, wherein the subject has an increased ratio of ZPPIX to free protoporphyrin IX (ZPPIX / PPIX ratio) compared to a subject with EPP. (Item 26) 23. The method of any one of items 1 to 22, wherein the subject has a uroporphyrin I and / or coproporphyrin I level that is at least 10%, 20%, 30%, 40% or 50% higher than the uroporphyrin I and / or coproporphyrin I level in a healthy subject prior to administration of the GlyT1 inhibitor. (Item 27) 23. The method according to any one of items 1 to 22, wherein the subject has a 5-ALA level that is at least 10%, 20%, 30%, 40% or 50% higher than the 5-ALA level in a healthy subject prior to administration of the GlyT1 inhibitor. (Item 28) 28. The method of any one of items 1 to 27, wherein the subject's PPIX level is reduced, while the patient's heme level is substantially maintained. (Item 29) 29. The method of any one of items 1-28, wherein the patient's PPIX level is decreased by at least 50% (e.g., 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or at least 100%) and the patient's heme level is not decreased by more than 10% (e.g., 10%, 15%, 20%, 25% and 30%). (Item 30) 30. The method of claim 28 or 29, wherein the patient's PPIX level is reduced by at least 85% and the patient's heme level is not reduced by more than 15%. (Item 31) 30. The method according to any one of items 1 to 29, wherein the heme level is not decreased by more than 10% (e.g., 10%, 15%, 20%, 25% and 30%). (Item 32) 32. The method according to any one of items 1 to 31, wherein administering the pharmaceutical composition does not cause a substantial reduction in heme levels. (Item 33) The method according to any one of items 1 to 8, 10 to 15, 17 to 25 and 27 to 32, wherein the subject has an increased free protoporphyrin IX level in red blood cells. (Item 34) The method according to any one of items 1 to 8, 10 to 15, 17 to 25 and 27 to 33, wherein the method reduces free protoporphyrin IX levels in the subject. (Item 35) 35. The method of any one of items 1-8, 10-15, 17-25, and 27-34, wherein the method reduces free protoporphyrin IX levels in the subject by at least 10% (e.g., 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or at least 100%). (Item 36) The method according to any one of items 1 to 8, 10 to 15, 17 to 25 and 27 to 35, wherein the subject has an increased protoporphyrin IX level in stool. (Item 37) The method reduces protoporphyrin IX levels in the feces of the subject. The method according to any one of items 1 to 8, 10 to 15, 17 to 25 and 27 to 36. (Item 38) 38. The method of any one of items 1-8, 10-15, 17-25 and 27-37, wherein the method reduces protoporphyrin IX levels in the subject's stool by at least 10% (e.g., 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or at least 100%). (Item 39) 39. The method according to any one of items 1 to 38, wherein the subject's plasma porphyrins fluoresce with a peak at 634 nm when irradiated with blue light (e.g., light of 400 to 420 nm). (Item 40) 40. The method according to any one of items 1 to 39, wherein the subject's plasma porphyrins emit fluorescence with a peak at 626 nm to 634 nm when irradiated with blue light (e.g., light of 400 to 420 nm). (Item 41) 39. The method according to any one of items 1 to 38, wherein the subject's skin porphyrin fluoresces with a peak at 632 nm when irradiated with blue light (e.g., light of 400 to 420 nm). (Item 42) 39. The method according to any one of items 1 to 38, wherein the subject's skin porphyrin emits fluorescence with a peak at 626 nm to 634 nm when irradiated with blue light (e.g., light of 400 to 420 nm). (Item 43) The method according to any one of items 1 to 8, 10 to 15, 17 to 25 and 27 to 42, wherein the subject has an increased protoporphyrin IX level in the skin. (Item 44) The method according to any one of items 1 to 8, 10 to 15, 17 to 25 and 27 to 43, wherein the method reduces protoporphyrin IX levels in the skin of the subject. (Item 45) 45. The method of any one of items 1-8, 10-15, 17-25 and 27-44, wherein the method reduces protoporphyrin IX levels in the skin of the subject by at least 10% (e.g., 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or at least 100%). (Item 46) The method according to any one of items 1 to 8, 10 to 15, 17 to 25 and 27 to 45, wherein the subject has a protoporphyrin IX level in the skin higher than 0.2 FluoDerm Units (FDU). (Item 47) The method according to any one of items 1 to 8, 10 to 15, 17 to 25 and 27 to 45, wherein the subject has a protoporphyrin IX level in the skin higher than 1.0 FDU. (Item 48) The method according to any one of items 1 to 8, 10 to 15, 17 to 25 and 27 to 45, wherein the subject has a protoporphyrin IX level in the skin of between 1.0 FDU and 2.5 FDU. (Item 49) The method according to any one of items 1 to 8, 10 to 15, 17 to 25 and 27 to 45, wherein the subject has a protoporphyrin IX level in the skin higher than 2.5 FDU. Law. (Item 50) 50. The method according to any one of items 1-8, 10-15, 17-25 and 27-49, wherein the method reduces protoporphyrin IX levels in the skin of the subject to less than 0.5 FDU. (Item 51) 50. The method of any one of items 1-8, 10-15, 17-25 and 27-49, wherein the method reduces protoporphyrin IX levels in the skin of the subject to less than 1.0 FDU. (Item 52) 50. The method according to any one of items 1 to 8, 10 to 15, 17 to 25 and 27 to 49, wherein the method reduces protoporphyrin IX levels in the skin of the subject to less than 1.5 FDU. (Item 53) 50. The method of any one of items 1-8, 10-15, 17-25 and 27-49, wherein the method reduces protoporphyrin IX levels in the skin of the subject to less than 2.0 FDU. (Item 54) 50. The method according to any one of items 1-8, 10-15, 17-25 and 27-49, wherein the method reduces protoporphyrin IX levels in the skin of the subject to less than 2.5 FDU. (Item 55) The method according to any one of items 1 to 8, 10 to 15, 17 to 25 and 27 to 54, wherein the subject has an increased protoporphyrin IX level in red blood cells. (Item 56) The method according to any one of items 1 to 8, 10 to 15, 17 to 25 and 27 to 55, wherein the method reduces protoporphyrin IX levels in red blood cells of the subject. (Item 57) 57. The method of any one of items 1-8, 10-15, 17-25 and 27-56, wherein the method reduces protoporphyrin IX levels in red blood cells of the subject by at least 10% (e.g., 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or at least 100%). (Item 58) The subject has a -1 The method according to any one of items 1 to 8, 10 to 15, 17 to 25 and 27 to 57, wherein the patient has a protoporphyrin IX level in red blood cells higher than that of the control. (Item 59) The subject has a -1 ~53μmolL -1 The method according to any one of items 1 to 8, 10 to 15, 17 to 25 and 27 to 58, wherein the protoporphyrin IX level in red blood cells is 0.05 to 0.5%. (Item 60) The subject has a -1 The method according to any one of items 1 to 8, 10 to 15, 17 to 25 and 27 to 58, wherein the patient has a protoporphyrin IX level in red blood cells higher than that of the control. (Item 61) The method comprises increasing the protoporphyrin IX level in red blood cells of the subject to 53 μmol L -1 The method according to any one of items 1 to 8, 10 to 15, 17 to 25 and 27 to 58, wherein the concentration of 1 or 2 is reduced to a level below 1 or 2. (Item 62) The method comprises increasing the protoporphyrin IX level in red blood cells of the subject to 31 μmol L -1 The method according to any one of items 1 to 8, 10 to 15, 17 to 25 and 27 to 58, wherein the concentration of 1 or 2 is reduced to a level below 1 or 2. (Item 63) The method further comprises increasing the protoporphyrin IX level in red blood cells of the subject to 15 μmol L -1 The method according to any one of items 1 to 8, 10 to 15, 17 to 25 and 27 to 58, wherein the concentration of 1 or 2 is reduced to a level below 1 or 2. (Item 64) The method according to any one of items 1 to 7, 10 to 14, 17 to 25 and 27 to 63, wherein the subject's ferrochelatase activity level is reduced to 10 to 35% of the ferrochelatase activity level observed in a normal subject. (Item 65) The method according to any one of items 1 to 7, 10 to 14, 17 to 25 and 27 to 64, wherein the subject's ferrochelatase activity level is reduced to less than 50% of the ferrochelatase activity level observed in a normal subject. (Item 66) 64. The method according to any one of items 1 to 6, 8, 10 to 15, 17 to 25 and 27 to 63, wherein the subject has a gain-of-function mutation in ALAS2. (Item 67) 67. The method of any one of items 1 to 6, 8, 10 to 15, 17 to 25, 27 to 63 and 66, wherein ALAS2 enzyme activity in said subject is increased. (Item 68) 70. The method of any one of items 1-6, 8, 10-15, 17-25, 27-63, 66 and 67, wherein the subject has increased zinc protoporphyrin IX levels in red blood cells. (Item 69) 70. The method of any one of items 1-6, 8, 10-15, 17-25, 27-63 and 66-68, wherein the method reduces zinc protoporphyrin IX levels in red blood cells of the subject. (Item 70) 70. The method of any one of items 1-6, 8, 10-15, 17-25, 27-63, and 66-69, wherein the method reduces zinc protoporphyrin IX levels in red blood cells of the subject by at least 10% (e.g., 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or at least 100%). (Item 71) The method according to any one of items 1 to 5, 9 to 13, 16 to 23, 26, 27, 31, 32 and 39 to 42, wherein the subject has reduced activity of uroporphyrinogen III synthase. (Item 72) 72. The method of any one of items 1-5, 9-13, 16-23, 26, 27, 31, 32, 39-42 and 71, wherein the subject has an increased level of uroporphyrin I and / or coproporphyrin I. (Item 73) 73. The method of claim 72, wherein the increased levels of uroporphyrin I and / or coproporphyrin I are measured in the urine or red blood cells of the subject. (Item 74) 73. The method of claim 72, wherein the increased level of coproporphyrin I is measured in the subject's feces. (Item 75) 75. The method according to any one of items 1-5, 9-13, 16-23, 26, 27, 31, 32, 39-42 and 71-74, wherein the method reduces the level of uroporphyrin I and / or coproporphyrin I in the subject. (Item 76) 75. The method according to any one of items 1-5, 9-13, 16-23, 26, 27, 31, 32, 39-42 and 71-74, wherein the method reduces the level of uroporphyrin I in the subject. (Item 77) 77. The method of any one of items 1-5, 9-13, 16-23, 26, 27, 31, 32, 39-42, and 71-76, wherein the method reduces the level of uroporphyrin I in the subject by at least 10% (e.g., 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or at least 100%). (Item 78) 78. The method according to any one of items 1-5, 9-13, 16-23, 26, 27, 31, 32, 39-42 and 71-77, wherein the method reduces the level of coproporphyrin I in the subject. (Item 79) 80. The method of any one of paragraphs 1-5, 9-13, 16-23, 26, 27, 31, 32, 39-42, and 71-78, wherein the method reduces the level of coproporphyrin I in the subject by at least 10% (e.g., 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or at least 100%). (Item 80) 80. The method of any one of items 1-5, 9-13, 16-23, 26, 27, 31, 32, 39-42 and 71-79, wherein the subject has a mutation in UROS. (Item 81) 81. The method of any one of items 1-5, 9-13, 16-23, 26, 27, 31, 32, 39-42 and 71-80, wherein the subject has a genetic defect in the GATA-1 erythroid-specific transcription factor. (Item 82) The method according to any one of items 1 to 5, 9 to 13, 16 to 23, 26, 27, 31, 32, 39 to 42 and 71 to 81, wherein the subject has red fluorescent urine. (Item 83) The method according to any one of items 1 to 5, 9 to 13, 16 to 23, 26, 27, 31, 32, 39 to 42 and 71 to 82, wherein the subject has a peak at 615 nm to 620 nm using plasma porphyrin fluorescence analysis. (Item 84) 84. The method according to any one of items 1 to 83, wherein the subject has a liver disease associated with EPP, XLPP or CEP. (Item 85) 76. The method according to any one of items 1 to 75, wherein the liver disease associated with EPP, XLPP or CEP is cholelithiasis. (Item 86) 76. The method according to any one of items 1 to 75, wherein the liver disease associated with EPP, XLPP or CEP is a mild liver disease. (Item 87) 76. The method according to any one of items 1 to 75, wherein the liver disease associated with EPP, XLPP or CEP is an exacerbation of liver disease. (Item 88) 76. The method according to any one of items 1 to 75, wherein the liver disease associated with EPP, XLPP or CEP is end-stage liver disease. (Item 89) 89. The method of any one of items 1-88, further comprising administering to the subject an additional active agent and / or supportive care. (Item 90) 90. The method of item 89, wherein the additional active agent and / or supportive therapy is selected from the group consisting of sun avoidance, topical sunscreens, skin protection, UVB phototherapy, afamelanotide (Scenesse®), bortezomib, proteasome inhibitors, chemical chaperones, cholestyramine, activated charcoal, iron supplementation, liver transplant, bone marrow transplant, splenectomy, and blood transfusion. (Item 91) The GlyT1 inhibitor is [ka] [In the formula, Ar is an unsubstituted or substituted aryl or 6-membered heteroaryl containing 1, 2 or 3 nitrogen atoms, wherein the substituted aryl and heteroaryl groups are hydroxy, halogen, NO 2 ,CN,(C 1 ~C 6 )-alkyl, halogen-substituted (C 1 ~C 6 )-alkyl, hydroxy substituted (C 1 ~C 6 )-alkyl, (CH 2 )n-(C 1 ~C 6 )-alkoxy, halogen-substituted (C 1 ~C 6 )-Alkoxy, NR 7 R 8 , C(O)R 9 , SO2R10 and -C(CH 3 )=NOR 7 or (C 1 ~C 6 )-substituted by a 5-membered aromatic heterocycle containing 1 to 4 heteroatoms selected from N and O, optionally substituted by alkyl; R 1 is hydrogen or (C 1 ~C 6 )-alkyl; R 2 is hydrogen, (C 1 ~C 6 )-alkyl, (C 2 ~C 6 )-alkenyl, halogen-substituted (C 1 ~C 6 )-alkyl, hydroxy substituted (C 1 ~C 6 )-alkyl, (C 1 ~C 6 )-alkoxy or halogen optionally substituted (CH2)n-(C 3 ~C 7 )-cycloalkyl, CH(CH 3 )-(C 3 ~C 7 )-cycloalkyl, (CH 2 ) n+1 -C(O)-R 9 , (CH 2 ) n+1 -CN, bicyclo[2.2.1]heptyl, (CH 2 ) n+1 -O-(C 1 ~C 6 )-alkyl, (CH 2 ) n -heterocycloalkyl, (CH 2 ) n -aryl or (CH 2 ) n - 5- or 6-membered heteroaryl containing 1, 2 or 3 heteroatoms selected from the group consisting of oxygen, sulfur or nitrogen (CH 2 )n - 5- or 6-membered heteroaryl, where aryl, heterocycloalkyl and heteroaryl are unsubstituted or substituted with hydroxy, halogen, (C 1 ~C 6 )-Alkyl and (C 1 ~C 6 )-alkoxy; R 3 , R 4 and R 6 are each independently hydrogen, hydroxy, halogen, (C 1 ~C 6 )-alkyl, (C 1 ~C 6 )-alkoxy or O-(C 3 ~C 6 )-Cycloalkoxy le; R 5 No 2 , C.N., C(O)R. 9 or SO 2 R 10 and; R 7 and R 8 are each independently hydrogen or (C1-C6)-alkyl; R 9 is hydrogen, (C 1 ~C 6 )-alkyl, (C 1 ~C 6 )-alkoxy or NR 7 R 8 and; R 10 is optionally substituted with halogen (C 1 ~C 6 )-alkyl, (CH 2 ) n -(C 3 ~C 6 )-cycloalkyl, (CH 2 ) n -(C 3 ~C 6 )-alkoxy, (CH 2 ) n-heterocycloalkyl or NR 7 R 8 and; n is 0, 1 or 2. or a pharma- ceutically acceptable salt thereof, or a prodrug of said compound or a pharma- ceutically acceptable salt thereof. (Item 92) The GlyT1 inhibitor is [ka] or a pharma- ceutically acceptable salt thereof, or a prodrug of said compound or a pharma- ceutically acceptable salt thereof. (Item 93) 93. The method according to any one of items 1 to 92, wherein the pharmaceutical composition further comprises a pharma- ceutically acceptable carrier. (Item 94) 94. The method according to any one of items 1 to 93, wherein the subject is in need thereof. (Item 95) 95. The method according to any one of items 1 to 94, wherein the GlyT1 inhibitor or a pharma- ceutically acceptable salt thereof, or a prodrug of the GlyT1 inhibitor or a pharma- ceutically acceptable salt thereof, is administered in a therapeutically effective amount.

Claims

[Claim 1] The invention described in this specification.

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