Method, composition, and device for isolation and expression analysis of region of interest from tissue

JP2025081588A5Pending Publication Date: 2025-10-22QUANTUMCYTE
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Patent Information

Application Number
JP2025026678
Authority / Receiving Office
JP · JP
Patent Type
Applications
Current Assignee / Owner
Priority Date
2018-06-28
Filing Date
2025-02-21
Publication Date
2025-10-22

AI Technical Summary

Technical Problem

Conventional clinical analysis of gene and protein expression from tissue samples is hindered by the lack of a selection mechanism, resulting in composite expression profiles and DNA/RNA sequence data from various cell types within the tissue.

Method used

A method for isolating biomolecules from specific target regions in a flat tissue section on a solid substrate, involving the delineation of a boundary with a polymer to form a non-physical fluid barrier, followed by selective lysis of cells within the target region, allowing for the isolation and analysis of biomolecules without physical barriers.

Benefits of technology

Enables the precise isolation and analysis of biomolecules from specific regions of interest within a tissue section, improving the accuracy and specificity of gene and protein expression analysis, and allowing for the retention of spatial position information of the proteins or nucleic acids.

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Abstract

To provide a method for isolating cellular components from at least one region of interest in a planar tissue section.SOLUTION: A method is in-situ, and does not require prearrangement of immobilized nucleotide arrays for defining regions from a tissue. The method may include: preparing in situ a hydrophobic mask on a tissue section to isolate regions of interest, and subsequently, using a surface tension array as necessary to add and / or remove extraction and / or analysis reagents from particular regions of interest circumscribed by the hydrophobic mask.SELECTED DRAWING: Figure 4
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