Plant growth regulating agent containing viable cells or culture of bacillus-genus strain as active ingredient, and usage method therefor

Abstract

To provide a microbial material that is naturally derived, can be safely used, and can contribute to improvement of agricultural production quantity by increasing yield through regulating the growth of crops and imparting resistance to environmental stress, while simultaneously preventing plant disease.SOLUTION: The present invention provides a plant growth regulating agent and / or a plant disease prevention agent that comprises viable cells of a specific strain belonging to the Bacillus genus, or a culture including the viable cells, as an active ingredient, thereby regulating the growth of crops, and also increases yield by imparting resistance to environmental stress.SELECTED DRAWING: None

Description

Technical Field

[0001] The present invention relates to a plant growth regulator containing a viable bacterium or culture of a Bacillus strain as an active ingredient and its use.

Background Art

[0002] With the global population increasing and desertification progressing on a global scale, it has become more important to increase crop yields. In addition, the progressive climate change due to global warming is predicted, and it is also important to endow crops with resistance to environmental variations. So far, increases in yields have been achieved through varietal improvement, improvement of fertilization techniques and cultivation techniques, etc., but the need for new tools with higher versatility is increasing. In the field of agricultural chemicals, the development of plant growth regulators has been carried out, but due to the increasing concern about environmental problems, there is a great demand for materials derived from natural products.

[0003] Among natural products, as materials using microorganisms, in recent years, Mamrich (registered trademark) for soybeans and Yume Bio (registered trademark) for paddy rice have been developed, but the applicable crops are limited.

[0004] On the other hand, among Bacillus species, there are strains having disease control activity and they have already been widely used for disease control.

[0005] Regarding some strains of Bacillus bacteria used for disease control, it has been found that they have an effect of promoting the germination rate on plant seeds (Patent Document 1), but it is not known that Bacillus bacteria directly increase the yield of crops.

[0006] Under such a technical background, as a measure to increase crop yields, as a result of investigations from various aspects, the present inventors focused on the plant growth regulating action and the plant disease control action, and keenly felt the necessity of developing an agent capable of performing at least one of these actions. Moreover, at that time, from the perspective of SDGs such as ensuring safety and preventing environmental pollution, the necessity of developing agents derived from natural products was keenly felt, and the development of these materials was also an object of the present invention.

[0007] On the one hand, the problem of damage to agricultural crops caused by diseases remains significant. Although comprehensive measures have been taken, such as crop rotation, cultivation using solar heat or physical control, chemical control using chemical pesticides, and control by using disease-resistant varieties, they are still not sufficient. Chemical control using chemical pesticides may not achieve sufficient control effects due to the emergence of drug-resistant bacteria, which has become a major problem globally.

[0008] In order to solve the so-called new technical problems described above, as a result of investigations from various aspects, it was concluded that microorganisms are suitable as the active ingredient of a new plant growth regulator. As a result of intensive screening, the Bacillus sp. D747 strain, which is a bacterium of the genus Bacillus, was obtained, and it was successful in newly developing a plant growth regulator containing the live bacteria or culture of this strain as the active ingredient.

[0009] On the other hand, as represented by global warming, environmental changes have been particularly significant recently. While desertification due to drought is progressing, natural disasters such as waterlogging of cultivated land due to heavy rain and rising sea levels are occurring frequently. Therefore, the burden of environmental stress on crops cannot be avoided. For example, the former causes high-temperature stress and the latter causes water stress, which harm the crops. As countermeasures against this, for example, irrigation, greening of deserts, and drainage such as surface drainage and subsurface drainage have been used to deal with the latter. Also, crop breeding and the like have been carried out. However, these conventional methods have unavoidable drawbacks such as high costs for methods using agricultural engineering like the former and long time required to complete for methods using breeding like the latter.

[0010] Regarding Bacillus sp. D747, which is the active ingredient of the plant growth regulator according to the present invention, as a result of further investigations by the present inventors, in the process of research, quite unexpectedly, it was first found that this strain has a completely new and extremely effective action and property of imparting resistance to environmental stress of crops.

[0011] Based on the above new findings and through further research, the present invention has been completed, and it also provides a novel and useful agent for conferring resistance to environmental stress in a new type of crop that is not only safe but also highly secure.

Prior Art Documents

Patent Documents

[0012]

Patent Document 1

Non-Patent Documents

[0013]

Non-Patent Document 1

Non-Patent Document 2

Summary of the Invention

Problems to be Solved by the Invention

[0014] The present invention aims to provide a natural-derived and safely usable microbial material that can contribute to the improvement of agricultural production volume by regulating the growth of crops, increasing the yield by conferring resistance to environmental stress, and simultaneously controlling plant diseases.

[0015] Due to the impact of global warming, which has become a particularly serious problem in recent years, the occurrence of pests and diseases in crops has been increasing. To address this, the use of powerful pesticides is required. On the other hand, due to the strong high-temperature stress caused by global warming, it is necessary to endow crops with resistance. In addition to clearing these issues to increase crop yields, the inventors have set a new task of creating a new type of material that has advanced functions such as plant growth regulation and plant disease control, and that uses naturally-derived microorganisms as active ingredients from the perspective of safety. Thus, the present invention has set an extremely advanced task of solving numerous conditions at once, and it can be said that the object of the invention, that is, the problem to be solved itself, is novel. In other words, the problem to be solved is to achieve both the "powerful control effect corresponding to the increase in pests and diseases" and the "granting of resistance to high-temperature stress of plants" caused by global warming, and at the same time, to achieve the granting of resistance to various environmental stresses such as the "granting of resistance to water stress corresponding to the increase in waterlogging, etc." caused by global warming.

Means for Solving the Problem

[0016] To achieve the above object, as a result of investigations from various aspects, the inventor found that the viable bacteria or cultures of Bacillus bacteria used for disease control can regulate the growth of crops, and in addition, have the effect of increasing yields by conferring resistance to environmental stress, and found a method of using this effect, thus completing the present invention.

[0017] That is, the embodiments of the present invention are as follows. (1) A plant growth regulator characterized by containing a viable bacterium or culture of Bacillus subtilis or Bacillus amyloliquefaciens as an active ingredient and having a plant growth promoting effect or a growth inhibiting effect. (2) It contains one or more viable bacteria or cultures of any one of Bacillus sp. D747 strain, Bacillus subtilis HAI-0404 strain, Bacillus subtilis Y1336 strain, and Bacillus amyloliquefaciens F727 strain as active ingredients, and has a plant growth promoting effect or a growth inhibiting effect. A plant growth regulator characterized by this. (3) It contains the viable bacteria or culture of Bacillus sp. D747 strain as an active ingredient, and has a plant growth promoting effect or a growth inhibiting effect. A plant growth regulator characterized by this. (4) In addition to the plant growth promoting effect or the growth inhibiting effect, it further imparts environmental stress tolerance to plants. The agent according to any one of (1) to (3), characterized by this. (5) In addition to the plant growth promoting effect or the growth inhibiting effect, it further has a plant disease control effect. The agent according to any one of (1) to (4), characterized by this. (6) It is for growth regulation of cruciferous plants (including asteraceae plants). The agent according to any one of (1) to (5), characterized by this. (7) It is for growth regulation of solanaceous plants. The agent according to any one of (1) to (5), characterized by this. (8) It is for growth regulation of leguminous plants. The agent according to any one of (1) to (5), characterized by this. (9) It is for growth regulation of brassicaceae plants. The agent according to any one of (1) to (5), characterized by this. (10) A plant growth regulation method and / or an environmental stress tolerance imparting method and / or a plant disease control method, characterized by including the step of bringing one or more plant growth regulators according to any one of (1) to (9) into contact with a plant body and / or soil (especially rhizosphere soil). (11) The method according to (10), characterized in that the plant body is a plant seed. (12) Seeds treated by the method according to (11). (13) The method according to (10), characterized in that the step of contacting with the soil is a perfusion treatment or a mixing treatment of the soil. (14) An agent for imparting environmental stress tolerance to plants, characterized by containing viable bacteria or a culture of Bacillus sp. strain D747 as an active ingredient. (15) The agent according to (14), characterized in that the environmental stress of the plant is a high temperature stress. (16) The agent according to (14), characterized in that the environmental stress of the plant is a water stress. (17) A method for imparting environmental stress tolerance to plants, characterized by using the agent according to (14). [Advantages of the Invention]

[0018] According to the present invention, by simply using a plant growth regulator containing viable bacteria or a culture of a single or multiple strains of natural products as an active ingredient, the growth of crops can be adjusted, the tolerance to environmental stress can be imparted to increase the yield, and plant diseases can also be controlled. That is, it is possible to provide a material that contributes to crop production in both aspects of plant growth regulation and suppression of yield reduction due to increased yield by imparting tolerance to environmental stress and suppression of yield reduction due to disease control.

[0019] The environmental stress in the present invention includes, but is not limited to, high temperature stress, low temperature stress, water stress, drought stress, salt stress, ultraviolet stress, wind stress, etc. In particular, high temperature stress and water stress can be mentioned. [Brief Description of the Drawings]

[0020]

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Mode for Carrying Out the Invention

[0021] As an active ingredient in the present invention, Bacillus sp. strain D747 is exemplified. This D747 strain was isolated from the air in Kikukawa City, Shizuoka Prefecture, and was internationally deposited as Bacillus sp. D747 (FERM BP-8234) on November 28, 2000 (Year 12 of Heisei) at the Patent Biological Depositary Center, National Institute of Advanced Industrial Science and Technology (currently, the Patent Microorganism Depositary Center, National Institute of Technology and Evaluation: 2-5-8 Kazusa Kamashima, Kisarazu City, Chiba Prefecture 292-0818, Japan). In addition, this strain is commercially available as described later, and can be easily obtained by anyone.

[0022] The bacterial properties of this Bacillus sp. D747 strain are as shown below. The tests for mycological properties were carried out based on Bergey's Manual of systematic Bacteriology, volume 1 (1984).

[0023] (A) Morphological properties Morphology: Bacillus Size: width 1.0 - 1.2 μm, length 3 - 5 μm Motility: + Arrangement of flagella: Peritrichous Endospores: + Position of spores: Central Swelling of spores: -

[0024] (B) Cultural properties Color of colonies: White to light brown Nutrient agar plate culture: Forms white to cream-colored colonies, and the surface is wrinkled

[0025] (C) Physiological properties Gram stainability: + Reduction of nitrate: + MR test: – VP test: + Indole production: – Starch hydrolysis: + Utilization of citrate: + Inorganic nitrogen source: + Oxidase: – Catalase: + Growth pH 6.8, meat extract medium: + 5.7, meat extract medium: + Growth temperature 30°C: + 50°C: – Growth NaCl concentration 2%: + 5%: + 7%: + Aerobic growth: + Anaerobic growth: – O-F test: O Yolk reaction: – Acid production from glucose: + Acid production from mannitol: – Acid production from L-arabinose: – Acid production from D-xylose: – Gas production from glucose: – β-galactosidase: – NaCl and KCl requirement: –

[0026] Incidentally, the Bacillus sp. D747 strain is commercially available under the trade name EcoShot (registered trademark). In addition to the D747 strain, for example, Bacillus subtilis HAI-0404 strain, Bacillus subtilis Y1336 strain, Bacillus amyloliquefaciens F727 strain, etc. can also be used in the present invention. The Bacillus subtilis HAI-0404 strain is commercially available under the trade name of Agrocare (registered trademark) wettable powder, the Bacillus subtilis Y1336 strain is commercially available under the trade name of Bactister (registered trademark) wettable powder, and the Bacillus amyloliquefaciens F727 strain is commercially available under the trade name of STARGUS (registered trademark). Incidentally, in this specification, there are notations such as "バシラス", "バチルス", "バチラス" in addition to "Bacillus", but these are all Japanese notations of the scientific name "Bacillus" and are the same.

[0027] What is exemplified in the present invention contains viable bacteria or a culture of Bacillus sp. D747 as an active ingredient. In the plant growth regulator of the present invention, the D747 strain can be used alone, but viable bacteria or a culture of a mutant of the D747 strain can also be used alone or together with viable bacteria or a culture of the D747 strain. The mutant has the bacteriological characteristics of the above D747 strain and has a plant growth regulating action, and natural mutants, mutants using ultraviolet rays or chemical mutagens, cell fusion strains, and gene recombinant strains can also be used. The D747 strain contained in the plant growth regulator of the present invention includes mutants of the D747 strain.

[0028] The culture used in the present invention is obtained from a culture of bacteria belonging to the above genus Bacillus. The culture refers to a mixture of microbial cells and a medium. The culture of bacteria belonging to the genus Bacillus can be carried out according to the usual culture methods of bacteria belonging to the genus Bacillus, such as reciprocating shaking culture, jar fermenter culture, liquid culture such as a culture tank, and solid culture. For example, in addition to general media such as meat extract medium, media containing glucose, peptone, and yeast extract can be mentioned. In addition to the liquid medium, solid media such as slant media and plate media containing agar may also be used. By culturing, bacteria belonging to the genus Bacillus can be grown to obtain a desired culture.

[0029] As the carbon source of the medium, any substance that can be assimilated by the above-mentioned strain can be used. Specifically, various synthetic or natural carbon sources that can be utilized by strains belonging to the genus Bacillus, such as glucose, arabinose, mannose, starch hydrolyzate, molasses, etc., can be mentioned. Similarly, as the nitrogen source of the medium, organic nitrogen-containing substances such as peptone, meat extract, yeast extract, soybean meal, etc., and various synthetic or natural substances that can be utilized by the strain are available. Furthermore, according to the conventional methods of microbial culture, inorganic salts such as sodium chloride and phosphate, salts of metals such as calcium, magnesium, and iron, trace nutrient sources such as vitamins, amino acids, and nucleic acid-related substances can also be added as necessary. Additionally, various additives such as antifoaming agents can be added as necessary.

[0030] The culture can be carried out under aerobic conditions such as shaking culture and aeration culture. The culture temperature is 20 to 30 °C, preferably 25 to 30 °C, the pH is 5 to 8, preferably 6 to 7, and the culture period is 1 to 4 days, preferably 2 to 3 days.

[0031] The Bacillus strain of the active ingredient of the present invention cultured as described above can be used as the active ingredient of a plant growth regulator in the state of a culture containing viable cells without separating the cells. Also, viable cells can be separated from the above-mentioned culture by ordinary methods, such as membrane separation or centrifugation, and the culture-separated viable cells themselves or their processed products (such as mixtures of culture-separated viable cells and other components) can be used as the active ingredient after washing as necessary. Furthermore, the above-mentioned culture or separated viable cells can also be used in the form of dried products dried by methods such as freeze-drying and spray-drying, or dilutions with these liquids and solids. It can also be used in a formulated state with various additives according to the conventional methods of agricultural chemical formulations. Such dosage forms include, for example, granules, emulsions, wettable powders, flowable agents, etc.

[0032] The concentration of viable cells contained in the plant growth regulator according to the present invention is not particularly limited as long as the desired effect is exhibited. However, if the bacterial concentration is too low, sufficient results cannot be obtained. Conversely, even if the bacterial concentration is increased too much, the bacteria may be wasted. For example, when prepared and used as a liquid preparation, it can be appropriately adjusted within the range of 1×10 5 ~1×10 11 cfu / ml, and preferably within the range of 1×10 6 ~1×10 10 cfu / ml. Here, in the present invention, "cfu" means Colony Forming Unit. In addition, even when using a culture, it may be appropriately designed according to the above viable cell concentration.

[0033] Plants expected to be growth-regulated, resistant to environmental stress, and have disease control by the plant growth regulator of the present invention include cereals (e.g., rice, wheat, barley, corn, buckwheat), tubers (e.g., potato, sweet potato, taro, yam), legumes (e.g., soybean, green bean, adzuki bean, pea), vegetables (e.g., cucurbits, tomato, eggplant, pepper, cabbage, Chinese cabbage, radish, lettuce, carrot, leek, onion, strawberry, spinach, sugar beet, beet, Brassica rapa var. parachinensis, celery), fruit trees (e.g., apple, pear, walnut, peach, grape, persimmon, citrus fruits, kiwi), special crops (e.g., cotton, rapeseed, sunflower, beet, sugarcane, tobacco), turfgrass, trees, ornamental plants (e.g., rose, chrysanthemum, tulip, baby's breath, campanula, etc.), etc. However, the present invention is not limited by these examples.

[0034] Examples of plant pathogens that can be controlled by the plant growth regulator and / or plant disease control agent and / or environmental stress inducer of the present invention include, specifically, bacteria of the genus Pseudoperonospora, such as Pseudoperonospora cubensis, bacteria of the genus Venturia, such as Venturia inaequalis, bacteria of the genus Erysiphe, such as Erysiphe graminis, bacteria of the genus Pyricularia, such as Pyricularia oryzae, bacteria of the genus Botrytis, such as Botrytis cinerea, bacteria of the genus Rhizoctonia, such as Rhizoctonia solani, bacteria of the genus Cladosporium, such as Cladosporium fulvum, bacteria of the genus Colletotrichum, such as Colletotrichum fragariae, bacteria of the genus Puccinia, such as Puccinia recondita, bacteria of the genus Septoria, such as Septoria nodorum, bacteria of the genus Sclerotinia, such as Sclerotinia sclerotiorum, bacteria of the genus Pythium, such as Pythium debaryanum Hesse, bacteria of the genus Gaeumannomyces, such as Gaeumannomyces graminis, and bacteria such as Peudomonas syringae, but are not limited to these examples.

[0035] The plant growth regulator and / or environmental stress tolerance-imparting agent and / or plant disease control agent according to the present invention can be directly applied as it is or diluted with water or the like before application. The application method as a chemical agent is not particularly limited, and examples thereof include a method of directly adhering to crops or seeds by spraying or dipping, a method of spraying on soil, a method of irrigating soil, a method of adding to water or fertilizer applied to crops or soil, and a method of treating agricultural implements. Among these, the method of directly spraying on crops is more preferable. Thus, by allowing the plant growth regulator and / or environmental stress tolerance-imparting agent and / or plant disease control agent according to the present invention to be present on the plant body such as roots, stems, leaves, seeds, etc., or in the cultivation soil thereof, the growth of the plant is adjusted, the tolerance to environmental stress is imparted, the yield is increased, and the diseases occurring in the plant are suppressed.

[0036] The dosage of the chemical agent of the present invention varies depending on the applicable crop, target disease, application method, occurrence tendency, degree of damage, environmental conditions, dosage form to be used, etc., and thus it is preferably adjusted as appropriate and cannot be generally specified. For example, a method of treating 10 ml to 1 L, preferably 50 ml to 1 L of a liquid agent per plant can be exemplified. Also, the application timing also varies depending on the target disease, dosage form, etc., but it is preferably appropriately treated within 2 weeks before and after planting. Furthermore, since the present invention is a microbial pesticide with no concern about the emergence of resistant bacteria to plant pathogens, continuous use for several days or consecutive use in continuous cropping is also possible.

[0037] Furthermore, the plant growth regulator and / or environmental stress tolerance-imparting agent and / or plant disease control agent according to the present invention can be used in combination with other fertilizers, agricultural chemicals, for example, fungicides, antiviral agents, insecticides, acaricides, nematicides, synergists, attractants, herbicides, plant growth regulators, etc., as necessary. In this case, it may be formulated and applied as a mixture under conditions that do not significantly affect the strain as the active ingredient, or they may be applied separately at different times or simultaneously.

[0038] Next, known fungicides (fungicidal active ingredients) or disease control agent compounds that may be mixed or used in combination are exemplified below, but are not limited to these examples.

[0039] Bactericidal active ingredient or disease control agent compound: Agrobacterim radiobacter, azaconazole, acibenzolar-S-methyl, azoxystrobin, anilazine, amisulbrom, aminopyrifen, ametoctradin, aldimorph, isotianil, isopyrazam, isofetamid, isoflucypram, isoprothiolane, ipconazole, ipflufenoquin, ipfentrifluconazole, iprodione, iprovalicarb, iprobenfos, imazalil, iminoctadine-albesilate, iminoctadine-triacetate, imibenconazole, inpyrfluxam, imprimatin A, imprimatin B, edifenphos, etaconazole, etaboxam, ethirimol, ethoxyquin, etridiazole, enestroburin, enoxastrobin, epoxiconazole, organic oiloils), oxadixyl, oxazinylazole, oxathiapiprolin, oxycarboxin, oxine - copper, oxytetracycline, oxpoconazole - fumarate, oxolinic acid, copper dioctanoate, octhilinone, ofurace, orysastrobin, o - phenylphenol, kasugamycin, captafol, carpropamid, carbendazim, carboxin, carvone, Candida oleophila, Candida saitoana, quinoxyfen, quinofumelin, chinomethionat, captan, quinconazole, quintozene, guazatine, cufraneb, coumethoxystrobin, coumoxystrobin, Gliocradium catenulatum, Cryptococcus albidus, kresoxim - methyl, clozylacon, Clonostachys rosea, chlozolinate, chloroinconazide, chlorothalonil, chloroneb, Chaetomium cupreum, ConiothyriumConiothyrium minitans, cyazofamid, diethofencarb, diclocymet, dichlofluanid, dichlobentiazox, diclomezine, dicloran, dichlorophen, dithianon, diniconazole, diniconazole-M, zineb, dinocap, dipymetitrone, diphenylamine, difenoconazole, cyflufenamid, diflumetorim, cyproconazole, cyprodinil, simeconazole, dimethirimol, dimethyl disulfide, dimethomorph, cymoxanil, dimoxystrobin, Pseudozyma flocculosa, Pseudomonas aureofaciens, Pseudomonas chlororaphis, Pseudomonas syringae, Pseudomonas flurorescens, Pseudomonas rhodesiae, ziram, silthiofam, Zucchini yellow mosaic virus, streptomycin, Streptomyces griseoviridis, StreptomycesStreptomyces lygicus, spiroxamine, sedaxane, seboctylamine, zoxamide, solatenol, dazomet, Talaromyces flavus, tiadinil, thiabendazole, thiram, thiophanate, thiophanate-methyl, thifluzamide, thiram, tecnazene, tecloftalam, tetraconazole, debacarb, tebuconazole, tebufloquin, terbinafine, dodine, dodemorph, triadimenol, triadimefon, triazoxide, trichlamide, triclopyricarb, Trichoderma asperellum, Trichoderma atroviride, Trichoderma gamsii, Trichoderma stromaticum, Trichoderma harzianum, Trichoderma viride, Trichoderma virens, Trichoderma polysporum, Trichoderma lignorum (Trichodermalignorum), tricyclazole, triticonazole, tridemorph, triflumizole, trifloxystrobin, triforine, tolylfluanid, tolclofos-methyl, tolnifanide, tolprocarb, nabam, natamycin, naftifine, nitrapyrin, nitrothal-isopropyl, nuarimol, copper nonyl phenol sulphonate, Paenibacillus polymyxa, Barkholderia cepacia, Bacillus amyloliquefaciens, Bacillus simplex, Bacillus subtilis, Bacillus pumilus, Bacillus licheniformis, harpin protein, Variovorax paradoxus, validamycin, valifenalate, Pantoea agglomerans, picarbutrazox, bixafen, picoxystrobin, Pythium oligandrum, pydiflumetofen, bitertanol, binapacryl, hinokitiol, non-pathogenic Erwinia carotovoracarotovora), non-pathogenic Rhizobium vitis, biphenyl, piperalin, hymexazol, pyraoxystrobin, pyraclostrobin, pyraziflumid, pyrazophos, pyrapropoyne, pyrametostrobin, pyriofenone, pyrisoxazole, pyridachlometyl, pyrifenox, pyributicarb, pyriben carb), pyrimethanil, pyroquilon, vinclozolin, ferbam, famoxadone, phenazine oxide, fenamidone, fenaminstrobin, fenarimol, fenoxanil, ferimzone, fenpiclonil, fenpicoxamid, fenpyrazamine, fenbuconazole, fenfuram, fenpropidin, fenpropimorph, fenhexamid, folpet, phthalide, Fusarium oxysporum, bupirimate, fuberidazole, blasticidin-S, furametpyr, furalaxyl, furancarboxylic acid, fluazinam, fluindapyr, fluoxastrobin, fluoxapiprolin, fluoxytioconazole, fluopicolide, fluopimomide, fluopyram, fluoroimide, fluxapyroxad, fluquinconazole, furconazole, furconazole-cis-cis), fludioxonil, flusilazole, flusulfamide, flutianil, flutolanil, flutriafol, flufenoxadiazam, flufenoxystrobin, flubeneteram, flumetylsulforim, flumetover, flumorph, Phlebiopsis giganteagigantea), proquinazid, prochloraz, procymidone, prothiocarb, prothioconazole, bronopol, propamocarb - hydrochloride, propiconazole, propineb, probenazole, bromuconazole, flometoquin, florylpicoxamid, hexaconazole, benalaxyl, benalaxyl - M, benodanil, benomyl, pefurazoate, penconazole, pencycuron, benzovindiflupyr, benthiazole, benthiavalicarb - isopropyl, penthiopyrad, penflufen, boscalid, fosetyl (including salts such as aluminum, calcium, sodium, etc.), polyoxin, polycarbamate, Bordeaux mixture, mancopper, mancozeb, mandipropamid, mandestrobin, maneb, myclobutanil, Mitsuaria chitosanitabida, mineral oiloils), mildiomycin, methasulfocarb, metam, metalaxyl, metalaxyl-M, metarylpicoxamid, metiram, metyltetraprole, metconazole, metominostrobin, metrafenone, mepanipyrim, mefentrifluconazole, meptyldinocap, mepronil, iodocarb, laminarin, phosphorous acid and salts, copper oxychloride, silver, copper(II) acetate, cuprous oxide, copper hydroxide, potassium bicarbonate, sodium bicarbonate, sulfur, oxyquinoline sulfate, copper sulfate, (3,4-dichloro-isothiazol-5-yl)methyl 4-(tert-butyl)benzoate (chemical name, CAS registration number: 1231214-23-5), UK-2A (code number), bis(ethylenediamine)copper(II) dodecylbenzenesulfonate [II] (DBEDC), triphenyltin acetate (TPTA), triphenyltin chloride (TPTC), triphenyltin hydroxide (TPTH).

[0040] Next, known insecticides (insecticidal active ingredients), acaricides (acaricidal active ingredients), nematicides (nematicidal active ingredients), and synergist compounds (synergistic active ingredients) that may be mixed or used in combination are exemplified below, but are not limited to these examples.

[0041] Insecticidal active ingredient, acaricidal active ingredient, nematocidal active ingredient, synergistic active ingredient: acrinathrin, azadirachtin, azamethiphos, acynonapyr, azinphos-ethyl, azinphos-methyl, acequinocyl, acetamiprid, acetoprole, acephate, azocyclotin, abamectin, afidopyropen, afoxolaner, amidoflumet, amitraz, alanycarb, aldicarb, aldoxycarb, allethrin [including d-cis-trans form, d-trans form], isazophos, isamidofos, isocarbophos, isoxathion, isocycloseram, isofenphos-methyl, isoprocarb, epsilon-metofluthrin, epsilon-momfluorothrin, ivermectin, imicyafos, imidacloprid, imiprothrin, indoxacarb, esfenvalerate, ethiofencarb, ethion, ethiprole, ethylene dibromide, etoxazole, etofenprox, ethoprophos, etrimfos, emamectin, emamectin benzoatebenzoate), endosulfan, empenthrin, oxazosulfyl, oxamyl, oxydemeton-methyl, oxydeprofos, omethoate, Nuclear polyhedrosis virus, cadusafos, kappa-tefluthrin, kappa-bifenthrin, karanjin, cartap, Granulosis virus, carbaryl, carbosulfan, carbofuran, gamma-BHC, xylylcarb, quinalphos, kinoprene, chinomethionat, Entero virus, coumaphos, cryolite, clothianidin, clofentezine, chromafenozide, chlorantraniliprole, chlorethoxyfos, chlordane, chloropicrin, chlorpyrifos, chlorpyrifos-methyl, chlorfenapyr, chlorfenvinphos, chlorfluazuron, chlormephos, chloroprallethrin, Entomopoxi virus, Iridovirus), Cyazypyr, Cyanophos, Diafenthiuron, Diamidafos, Cyantraniliprole, Cyetpyrafen, Dienochlor, Cyenopyrafen, Dioxabenzofos, Diofenolan, Sigma virus (Sigmavirus), cyclaniliprole, cycloxaprid, dicrotophos, dichlofenthion, cyclobutrifluram, cycloprothrin, dichlorvos, dicloromezotiaz, dicofol, dicyclanil, disulfoton, dinotefuran, dinobuton, cyhalodiamide, cyhalothrin [including gamma-form, lambda-form], cyphenothrin [including (1R)-trans-form], cyfluthrin [including beta-form], diflubenzuron, cyflumetofen, diflovidazin, cyproflanilide, cyhexatin, cypermethrin [including alpha-form, beta-form, theta-form, zeta-form], dimpropyridaz, dimethyl-2,2,2-trichloro-1-hydroxyethylphosphonate (DEP), dimethylvinphos, dimethoate, dimefluthrin, jasmone, cis-jasmone, jasmonic acid, methyl jasmonate, silafluofen, cyromazine, Steinernema carpocapsae, Steinernema kushidai, Steinernema glaseriglaseri), spidoxamat, spinetoram, spinosad, spirodiclofen, spirotetramat, spiropidion, spiromesifen, sulcofuron-sodium, sulfluramid, sulfoxaflor, sulfotep, diazinon, thiacloprid, thiamethoxam, tioxazafen, thiodicarb, thiocyclam, thiosultap, thionazin, thiofanox, thiometon, tyclopyrazoflor, tetrachlorantraniliprole, tetrachlorvinphos, tetradifon, tetraniliprole, tetramethylfluthrin, tetramethrin, tebupirimfos, tebufenozide, tebufenpyrad, tefluthrin, teflubenzuron, demeton-S-methyl, temephos, deltamethrin, terbufos, tralomethrin, transfluthrin, triazamate, triazophos, trichlorfon, Trichoderma asperellum, TrichodermaTrichoderma harzianum, triflumuron, triflumezopyrim, trimethacarb, tolfenpyrad, naled, nicotine, nicofluprole, nitenpyram, nemadectin, Denso virus, novaluron, noviflumuron, Paecilomyces lilacinus, Barkholderia cepacia, Barkholderia rinojensis, Verticillium lecanii, hydroprene, Pasteuria nishizawae, Pasteuria penetrans, Bacillus thuringiensis, insect toxins produced by Bacillus thuringiensis, Bacillus thuringiensis subsp. Aizawai, Bacillus thuringiensis subsp. Israelensis, Bacillus thuringiensis subsp. Kurstaki, Bacillus thuringiensis subsp. Tenebrionis, Bacillus popilliae, Bacillus licheniformislicheniformis), vamidothion, parathion, parathion-methyl, halfe nprox), halofenozide, bioallethrin, bioallethrin S-cyclopentenyl, resmethrin, bis-(2-chloro-1-methylethyl) ether (DCIP), bistrifluron, hydramethylnon, bifenazate, bifenthrin, pyflubumide, piperonyl butoxide, pymetrozine, pyraclofos, pyrafluprole, pyridaphenthion, pyridaben, pyridalyl, pyrifluquinazon, pyriprole, pyriproxyfen, pirimicarb, pyrimidifen, pyriminostrobin, pirimiphos-methyl, pyrethrine, famphur, fipronil, fenazaquin, fenamiphos, fenitrothion, fenoxycarb, fenothiocarb, phenothrin [(including the (1R)-trans isomer)], fenobucarb, fenthion, phenthoate, fenvalerate, fenpyroximate, fenbutatin oxide, fenpropathrin, fonofos, sulfuryl fluoridefluoride), butocarboxim, butoxycarboxim, buprofezin, furathiocarb, prallethrin, fluacrypyrim, fluazaindolizine, fluazuron, fluensulfone, fluopyram, sodium fluoroacetate(fluoroacetate), fluxametamide, flucycloxuron, flucythrinate, flusulfamide, fluthrin, fluvalinate [including tau-form], flupyradifurone, flupyrazofos, flupyrimin, flufiprole, flufenerim, flufenoxystrobin, flufenoxuron, fluhexafon, flubendiamide, flupentiofenox, flumethrin, fluralaner, flurimfen, prothiofos, protrifenbute, flonicamid, propaphos, propargite, prohydrojasmon, profenofos, broflanilide, profluthrin, propetamphos, propoxur, flometoquin, bromopropylate, hexythiazox, hexaflumuron, Paecilomyces tenuipes, Paecilomyces fumosoroceus, Paecilomyces lilacinuslilacinus), heptafluthrin, heptenophos, permethrin, benclothiaz, benzpyrimoxan, bensultap, benzoximate, bendiocarb, benfuracarb, Pochonia chlamydosporia, Beauveria tenella, Beauveria bassiana, Beauveria brongniartii, phoxim, phosalone, fosthiazate, fosthietan, phosphamidon, phosmet, polynactins, formetanate, phorate, machine oil, malathion, milbemectin, mecarbam, mesulfenfos, methomyl, metaldehyde, metaflumizone, methamidophos, metham, methiocarb, methidathion, methyl isothiocyanate, methyl bromide, methoxychlor, methoxyfenozide, methothrin, metofluthrin, methoprene, metolcarb, mevinphos, meperfluthrin, Monacrosporium fimato pagamPhymatophagum, Monacrosporium phymatophagum, monocrotophos, momfluorothrin, Trichoderma harzianum, litlure - A, litlure - B, aluminium phosphide, zinc phosphide, phosphine, lufenuron, rescalure, resmethrin, lepimectin, rotenone, Cytoplasmic polyhedrosis virus, fenbutatin oxide, calcium cyanide, organotins, nicotine - sulfate, (Z)-11 - tetradecenyl acetate, (Z)-11 - hexadecenal, (Z)-11 - hexadecenyl acetate, (Z)-9,12 - tetradecadienyl acetate, (Z)-9 - tetradecen - 1 - ol, (Z,E)-9,11 - tetradecadienyl acetate, (Z,E)-9,12 - tetradecadienyl acetate, 1,1,1 - trichloro - 2,2 - bis(4 - chlorophenyl)ethane (DDT), 1,3 - dichloropropene, 2,4 - dichloro - 5 - {2 - [4 - (trifluoromethyl)phenyl]ethoxy}phenyl 2,2,2 - trifluoroethyl sulfoxide (chemical name, CAS registration number: 1472052 - 11 - 1), 2,4 - dimethyl - 5 - [6 - (trifluoromethylthio)hexyloxy]phenyl - 2,2,2 - trifluoroethylSulfoxide (chemical name, CAS registration number: 1472050-34-2), 2-{2-fluoro-4-methyl-5-[(2,2,2-trifluoroethyl)sulfinyl]phenoxy}-5-(trifluoromethyl)pyridine (chemical name, CAS registration number: 1448758-62-0), 3-chloro-2-{2-fluoro-4-methyl-5-[(2,2,2-trifluoroethyl)sulfinyl]phenoxy}-5-(trifluoromethyl)pyridine (chemical name, CAS registration number: 1448761-28-1), 4,6-dinitro-o-cresol (DNOC), 4-fluoro-2-methyl-5-(5,5-dimethylhexyloxy)phenyl 2,2,2-trifluoroethyl sulfoxide (chemical name, CAS registration number: 1472047-71-4), Bt protein (Cry1Ab, Cry1Ac, Cry1Fa, Cry2Ab, mCry3A, Cry3Ab, Cry3Bb, Cry34 / 35Ab1), methyl eugenol, 4-(p-acetoxyphenyl)-2-butanone, (Z)-10-tetradecenyl acetate, (E,Z)-4,10-tetradecadienyl acetate, (Z)-8-dodecenyl acetate, (Z)-11-tetradecenyl acetate, (Z)-13-icosen-10-one, 14-methyl-1-octadecene, AKD-1193 (code number), BCS-AA10147 (code number), CL900167 (code number), O,O-diethyl-O-[4-(dimethylsulfamoyl)phenyl]-phosphorothionate (DSP), O-ethyl-O-4-(nitrophenyl)phenylphosphonothioate (EPN), RU15525 (code number), XMC (XMC), Z-13-icosen-10-one, ZXI8901 (code number), F4260 (code number).

[0042] Next, known herbicide compounds, herbicidal active ingredients, or plant growth regulator compounds that may be mixed or used in combination are exemplified below, but are not limited to these examples.

[0043] Herbicide compound or herbicidal active ingredient: Ioxynil (including salts with lithium salts, sodium salts, octanoic acid, etc.), Aclonifen, Acrolein, Azafenidin, Acifluorfen (including salts with sodium, etc.), Azimsulfuron, Asulam, Acetochlor, Atrazine, Anisiflupurin, Anilofos, Amicarbazone, Amidosulfuron, Amitrole, Aminocyclopyrachlor, Aminopyralid, Amiprofos-methyl, Ametryn, Araujia Mosaic Virus, Alachlor, Alternaria destructans (Alternariadestruens), alloxydim (including salts with sodium, etc.), ancymidol, isouron, isoxachlortole, isoxaflutole, isoxaben, Isodecylalkoholethoxylate, isoproturon, ipfencarbazone, imazaquin, imazapic (including salts with amines, etc.), imazapyr (including salts with isopropylamine, etc.), imazamethabenz, imazamethabenz-methyl, imazamox, imazethapyr, imazosulfuron, indaziflam, indanofan, eglinazine-ethyl, esprocarb, ethametsulfuron-methyl, ethalfluralin, ethidimuron, ethoxysulfuron, ethoxyfen, ethoxyfen-ethyl, ethofumesate, etobenzanid, epyrifenacil, endothal-disodium, oxadiazon, oxadiargyl, oxaziclomefone, oxasulfuron, oxyfluorfen, oryzalin, Obuda Pepper VirusVirus), orthosulfamuron, orbencarb, oleic acid, cafenstrole, caprylic acid, capric acid, carfentrazone-ethyl, karbutilate, carbetamide, quizalofop, quizalofop-ethyl, quizalofop-P-ethyl, quizalofop-P-tefuryl, Xanthomonas campestris, quinoclamine, quinclorac, quinmerac, citricacid), cumyluron, clacyfos, glyphosate (including salts such as sodium, potassium, amine, propylamine, isopropylamine, ammonium, isopropylammonium, guanidine, monoethanolamine, choline, BAPMA (N,N-bis-(aminopropyl)methylamine), dimethylamine or trimesium), glufosinate (including salts such as amine or sodium), glufosinate-P, glufosinate-P-sodium, clethodim, clodinafop, clodinafop-propargyl, clopyralid (including monoethanolamine salt), clomazone, chlomethoxyfen, clomeprop, cloransulam-methyl, chloramben, chloridazon, chlorimuron, chlorimuron-ethyl, chlorsulfuron, chlorthal-dimethyl, chlorthiamid, chlorphthalim, chlorflurenol-methyl, chlorpropham, chlorbromuron, chloroxuron, chlorotoluron, ketospiradox (including salts such as sodium, calcium or ammonia), Colletotrichum orbiculare, Colletotrichum gloeosporioides, Colletotrichum truncatumtruncatum), Chondrostercum purpureum, saflufenacil, sarmentine, cyanazine, cyanamide, diuron, diethatyl-ethyl, dioxopyritrione, dicamba (including salts such as amine, diethylamine, isopropylamine, diglycolamine, dimethylammonium, diolamine, isopropylammonium, oramine, potassium, trolamine, BAPMA (N,N-bis-(aminopropyl)methylamine), choline, sodium or lithium, or esters such as methyl ester), cycloate, cycloxydim, diclosulam, cyclosulfamuron, cyclopyranil, cyclopyrimorate, dichlobenil, diclofop, diclofop-P-methyl, diclofop-methyl, dichlorprop, dichlorprop-P (including salts such as dimethylammonium, potassium, sodium, choline, or esters such as butyl ester, 2-ethylhexyl ester, isooctyl ester, methyl ester), diquat, diquat dibromidedibromide), dithiopyr, siduron, dinitramine, cinidon-ethyl, cinosulfuron, dinoseb (including acetate), dinoterb, cyhalofop, cyhalofop-butyl, cypyrafluone, diphenamid, difenzoquat, diflufenican, diflufenzopyr, simazine, dimesulfazet, dimethachlor, dimethametryn, dimethenamid, dimethenamid-P, simetryn, dimepiperate, dimefuron, Pseudomonas fluorescens, cinmethylin, swep, sulcotrione, sulfentrazone, sulfosate, sulfosulfuron, sulfometuron-methyl, sethoxydim, Scelerothinia minor, terbacil, daimuron, thaxtomin A, Tobacco Mild Green Mosaic Tobamovirus, Tobacco RattleVirus), dalapon, thiazopyr, tiafenacil, thiencarbazone (including sodium salt, methyl ester, etc.), tiocarbazil, thiobencarb, thidiazimin, thidiazuron, thifensulfuron, thifensulfuron-methyl, desmedipham, desmetryne, tetflupyrolimet, thenylchlor, tebutam, tebuthiuron, tepraloxydim, tefuryltrione, terbuthylazine, terbutryn, terbumeton, tembotrione, topramezone, tralkoxydim, triaziflam, triasulfuron, triafamone, tri-allate, tri Trietazine, triclopyr, triclopyr-butotyl, triclopyr-triethylammonium, tritosulfuron, tripyrasulfone, trifludimoxazin, triflusulfuron-methyl, trifluralin, trifloxysulfuron (including salts such as sodium), tribenuron-methyl, tolpyralate, naptalam (including salts with sodium etc.), naproanilide, napropamide, napropamide-M, nicosulfuron, lactic acid, neburon, norflurazon, Burkholderia rinojensis, vernolate, paraquat, paraquat dichloride, halauxifen, halauxifen-benzyl, halauxifen-methyl, haloxyfop, haloxyfop-P, haloxyfop-etotyl, haloxyfop-P-methyl, halosafen, halosulfuron-methyl, bixlozone, picloram (including salts with dichloroammonium, trolamine etc.), picolinafen, bicyclopyronebispyribac-sodium, pinoxaden, bipyrazone, bifenox, piperophos, pyraclonil, pyrasulfotole, pyrazoxyfen, pyrazosulfuron-ethyl, pyrazolynate, bilanafos, pyraflufen, pyraflufen-ethyl, pyridafol, pyrithiobac-sodium, pyridate, pyriftalid, pyributicarb, pyribenzoxim, pyrimisulfan, pyriminobac-methyl, pyroxasulfone, pyroxsulam, Phytophthora palmivora, phenisopham, fenuron, fenoxasulfone, fenoxaprop (including methyl, ethyl, and isopropyl esters), fenoxaprop-P (including methyl, ethyl, and isopropyl esters), fenquinotrione, fenthiaprop-ethyl, fentrazamide, fenpyrazone, phenmedipham, Phoma chenopodicola, Phoma herbarum, Phoma macrostoma, butachlor, butafenacil, butamifos,butylate, Puccinia canaliculata, Puccinia thlaspeos, butenachlor, butralin, butroxydim, flazasulfuron, flamprop (including methyl, ethyl, and isopropyl esters), flamprop-M (including methyl, ethyl, and isopropyl esters), primisulfuron, primisulfuron-methyl, fluazifop-butyl, fluazifop-P, fluazifop-P-butyl, fluazolate, fluometuron, fluoroglycofen-ethyl, flucarbazone-sodium, fluchloralin, flucetosulfuron, fluthiacet-methyl, flupyrsulfuron-methyl (including salts such as sodium, calcium, or ammonia), flufenacet, flufenpyr-ethyl, flupropanate (including sodium salt), flupoxame, flumioxazin, flumiclorac-pentyl, flumetsulam, fluridone, flurtamone, fluroxypyr (including ester forms such as butomethyl and meptyl, or salts such as sodium, calcium, and ammonia), flurochloridone, pretilachlorProcarbazone (including salts with sodium, etc.), Prodiamine, Prosulfuron, Prosulfocarb, Propaquizafop, Propachlor, Propazine, Propanil, Propyzamide, Propisochlor, Propyrisulfuron, Propham, Profluazol, Prohexadione-calcium, Propoxycarbazone, Propoxycarbazone-sodium, Profoxydim, Bromacil, Brompyrazon, Prometryn, Prometon, Bromoxynil (including ester forms such as butyric acid, octanoic acid or heptanoic acid), Bromofenoxim, Bromobutide, Florasulam, Florpyrauxifen, Florpyrauxifen-benzyl, Hexazinone, Pethoxamid, Benazolin, Benazolin-ethyl, Penoxsulam, Pepino Mosaic Virus, Heptamaloxyloglucan, Beflubutamid, Beflubutamid-M, Pebulate, Pelargonic acid, Bencarbazone, Benquitrione, BenzfendizoneBensulide, bensulfuron, bensulfuron-methyl, benzobicyclon, benzofenap, bentazone, pentanochlor, pendimethalin, pentoxazone, benfluralin, benfuresate, fosamine, fomesafen, foramsulfuron, forchlorfenuron, Mecoprop (including salts such as sodium, potassium, isopropylamine, triethanolamine, dimethylamine, diolamine, trolamine, choline, etc., or esters such as etazyl ester, 2-ethylhexyl ester, isooctyl ester, methyl ester, etc.), Mecoprop-P-potassium, Mesosulfuron (including ester forms such as methyl), Mesotrione, Metazachlor, Metazosulfuron, Methabenzthiazuron, Metamitron, Metamifop, Metam (including salts such as sodium), Disodium methanearsonate (DSMA), Methiozolin, Methyldymuron, Metoxuron, Metosulam, Metsulfuron-methyl, Metobromuron, Metobenzuron, Metolachlor, Metribuzin, Mepiquat chloride, Mefenacet, Monosulfuron (including methyl, ethyl, isopropyl esters),Monolinuron, molinate, iodosulfuron, iodosulfuron-methyl-sodium, iofensulfuron, iofensulfuron-sodium, lactofen, (en), lancotrione, linuron, rimisoxafen, rimsulfuron, lenacil, 2,2,2-trichloroacetic acid (TCA) (including salts such as sodium, calcium or ammonia), 2,3,6-trichlorobenzoic acid (2,3,6-TBA), 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), 2,4-dichlorophenoxyacetic acid (2,4-D) (amines, diethylamine, triethanolamine, isopropylamine, dimethylammonium, diolamine, dodecylammonium, heptylammonium, tetradecylammonium, triethylammonium, tris(2-hydroxypropyl)ammonium, trolamine, choline, salts such as sodium or lithium, or esters such as butyl ester, 2-butoxypropyl ester, 2-ethylhexyl ester, methyl ester, ethyl ester, butyl ester, isobutyl ester, octyl ester, pentyl ester, propyl ester, isooctyl ester, isopropyl ester, meptyl ester, tefuryl ester, etc.), 2,4-dichlorophenoxybutyric acid (2,4-DB) (including salts such as amines, diethylamine, triethanolamine, isopropylamine, dimethylammonium, choline, sodium or lithium, or esters such as isooctyl ester), 2-amino-3-chloro-1,4-naphthoquinone (ACN), 2-methyl-4-chlorophenoxyacetic acid (MCPA) (salts such as sodium, dimethylammonium, choline, etc., or esters such as 2-ethylhexyl ester, isooctyl ester, ethyl ester, etc.), 2-methyl-4-chlorophenoxybutyric acid (MCPB) (including sodium salt, ethyl ester, etc.), 4-(2,4-dichlorophenoxy)butyric acid (2,4-DB), 4,6-dinitro-O-cresol (DNOC) (including salts such as amines or sodium), (5S)-3-(3,5-difluorophenyl)-N-[rel-(3R,5R)-5-(trifluoromethylsulfonylcarbamoyl)tetrahydrofuran-3-yl]-5-vinyl-4H-isoxazole-5-carboxamide ((5S)-3-(3,5-difluorophenyl)-N-[rel-(3R,5R)-5-(trifluoromethylsulfonylcarbamoyl)tetrahydrofuran-3-yl]-5-vinyl-4H-isoxazole-5-carboxamid)(Chemical name, CAS registration number: 2266183-40-6)(International Publication No. 2018 / 228986, International Publication No. 2020 / 114934), N4-(2,6-difluorophenyl)-6-(1-fluoro-1-methyl-ethyl)-1,3,5-triazine-2,4-diamine (N4-(2,6-difluorophenyl)-6-(1-fluoro-1-methyl-ethyl)-1,3,5-triazine-2,4-diamine)(Chemical name, CAS registration number: 1606999-43-2)(International Publication No. 2014 / 064094, International Publication No. 2015 / 162164), (5S)-3-(3,5-difluorophenyl)-N-[(3R)-5-(methylsulfonylcarbamoyl)-2,3-dihydrofuran-3-yl]-5-vinyl-4H-isoxazole-5-carboxamid ((5S)-3-(3,5-difluorophenyl)-N-[(3R)-5-(methylsulfonylcarbamoyl)-2,3-dihydrofuran-3-yl]-5-vinyl-4H-isoxazole-5-carboxamid)(Chemical name, CAS registration number: 2266190-06-9)(International Publication No. 2018 / 228986, International Publication No. 2020 / 114934), (5R)-3-(3,5-difluorophenyl)-5-methyl-N-[rel-(3R,5R)-5-(methylsulfonylcarbamoyl)tetrahydrofuran-3-yl]-4H-isoxazole-5-carboxamid ((5R)-3-(3,5-difluorophenyl)-5-methyl-N-[rel-(3R,5R)-5-(methylsulfonylcarbamoyl)tetrahydrofuran-3-yl]-4H-isoxazole-5-carboxamid)(Chemical name, CAS registration number: 2266164-36-5)(International Publication No. 2018 / 228986, International Publication No. 2020 / 114934), (5R)-3-(3,(5R)-3-(3,5-difluorophenyl)-N-[(3R)-5-(methoxycarbamoyl)-2,3-dihydrofuran-3-yl]-5-methyl-4H-isoxazole-5-carboxamide (chemical name, CAS registration number: 2266170-31-2) (International Publication No. WO 2018 / 228986, International Publication No. WO 2020 / 114934), 2-[2-(3,4-dimethoxyphenyl)-6-methyl-3-oxo-pyridazine-4-carbonyl]cyclohexane-1,3-dione (chemical name, CAS registration number: 2138855-12-4) (International Publication No. WO 2017 / 178582, International Publication No. WO 2018 / 015476), 4-hydroxy-1-methyl-3-[4-(trifluoromethyl)-2-pyridyl]imidazolidin-2-one (chemical name, CAS registration number: 1708087-22-2) (International Publication No. WO 2015 / 059262, International Publication No. WO 2018 / 015476), 6-(1-fluorocyclopentyl)-N4-(2,3,5,6-tetrafluorophenyl)-1,3,5-triazine-2,4-diamine (chemical name, CAS registration number: 1820807-75-7) (International Publication No. WO 2015 / 162164), 6-(1-fluoro-1-methyl-ethyl)-N4-(2,3,5,6-tetrafluorophenyl)-1,3,5-triazine-2,4-diamine5-triazine-2,4-diamine) (chemical name, CAS registration number: 1606999-21-6) (International Publication No. WO 2014 / 064094, International Publication No. WO 2015 / 162164), (5S)-3-(3-fluoro-5-methyl-phenyl)-N-[rel-(3R,5R)-5-(methoxycarbamoyl)tetrahydrofuran-3-yl]-5-vinyl-4H-isoxazole-5-carboxamide ((5S)-3-(3-fluoro-5-methyl-phenyl)-N-[rel-(3R,5R)-5-(methoxycarbamoyl)tetrahydrofuran-3-yl]-5-vinyl-4H-isoxazole-5-carboxamid) (chemical name, CAS registration number: 2266292-43-5) (International Publication No. WO 2018 / 228986, International Publication No. WO 2020 / 114934), 6-(1-methylcyclobutyl)-N4-(2,3,5,6-tetrafluorophenyl)-1,3,5-triazine-2,4-diamine (6-(1-methylcyclobutyl)-N4-(2,3,5,6-tetrafluorophenyl)-1,3,5-triazine-4,4-diamine) (chemical name, CAS registration number: 1607001-97-7) (International Publication No. WO 2014 / 064094, International Publication No. WO 2015 / 162164), AE-F-150944 (code number), F9960 (code number), IR-6396 (code number), MCPA-thioethyl, NC-656 (code number), SYP-298 (code number), SYP-300 (code number), S-ethyl dipropylthiocarbamate (EPTC), S-metolachlor, S-9750 (code number), MSMA (MSMA), HW-02 (code number), S-523 (code number), SL-1201 (code number).

[0044] Plant growth regulator: 1-Naphthylacetamide, 1-Methylcyclopropene, 1,3-Diphenylurea, 2,3,5-Triiodobenzoic Acid, 2-Methyl-4-chlorophenoxybutyric Acid [including sodium salt, ethyl ester, etc.], 2-(Naphthalene-1-yl)acetamide, 2,6-Diisopropylnaphthalene, 3-[(6-Chloro-4-phenylquinazoline-2-yl)amino]propane-1-ol, 4-Oxo-4-(2-phenylethyl)aminobutyric Acid (chemical name, CAS registration number: 1083-55-2), 4-Chlorophenoxyacetic Acid (4-CPA), 5-Aminolevulinic Acid Hydrochloride, Methyl 5-(trifluoromethyl)benzo[b]thiophene-2-carboxylate, AVG (aminoethoxyvinylglycine), n-Decanol, Anisiflupurin, Aviglycine, Ancymidol, Abscisic Acid, Isoprothiolane, Inabenfide, Indole Acetic Acid, Indole Butyric Acid, Uniconazole, Uniconazole-P, Ecolyst, Ethychlozate, Ethephon, Epocholeone, Calcium Chloride, Cholinechloride), oxine-sulfate, opabactin, kinetin, calcium peroxide, carvone, quinabactin, calcium formate, cloxyfonac, cloxyfonac-potassium, cloprop, chlormequat, chlormequat-chloride, chlorpropham, choline, cytokinins, oxidized glutathione, cyanamide, sodium cyanate, cyclanilide, dichlorprop (salts such as dimethylammonium, potassium, sodium, choline, etc., or esters such as butyl ester, 2-ethylhexyl ester, isooctyl ester, methyl ester, etc.), dichlorprop-P (salts such as sodium, potassium, dimethylammonium, etc., or including 2-ethylhexyl ester), diquat, diquat dibromide, dikegulac, gibberellinic acid, gibberellin A4, gibberellin A7, dimethipin, sintofen, jasmone, cis-jasmone, jasmonic acid, methyl jasmonate, streptomycin, calcium polysulfide, daminozide, calcium carbonatecarbonate), thidiazuron, decan-1-ol, triacontanol, triapenthenol, trinexapac-ethyl, tribufos, paclobutrazol, paraffin, bispyribac-sodium, hymexazol, butralin, fluthiacet-methyl, pyraflufen-ethyl, flumetralin, flurprimidol, flurenol, pronitridine, prohydrojasmon, prohexadione-calcium, heptamaloxyloglucan, 6-benzylaminopurine, pendimethalin, forchlorfenuron, formononetin, maleic hydrazide, mepiquat chloride, mefluidide, lipochitooligosaccharides (e.g., lipochitooligosaccharides SP104), calcium sulfate.

[0045] Next, known compounds for reducing phytotoxicity that may be mixed or used in combination are exemplified below, but are not limited to these examples.

[0046] Compound for reducing phytotoxicity: Isoxadifen, Isoxadifen-ethyl, Oxabetrinil, Octane-1,8-diamine, Cloquintocet, Cloquintcet-mexyl, Dietholate, Cyometrinil, Dichlormid, Dicyclonone, Cyprosulfamide, Daimuron, 1,8-Naphthalic Anhydride, Fenchlorazole, Fenchlorazole-O-ethyl, Fenclorim, Furilazole, Fluxofenim, Flurazole, Benoxacor, Metcamifen, Mephenate, Mefenpyr, Mefenpyr-ethyl, Mefenpyr-diethyl, Lower alkyl-substituted benzoic acid, 2,2-Dichloro-N-(1,3-dioxolan-2-ylmethyl)-N-(2-propenyl)acetamide (PPG-1292), 2-Dichloromethyl-2-methyl-1,3-dioxane (MG-191), 3-Dichloroacetyl-2,2,5-trimethyl-1,3-oxazolidine (R-29148), 4-Dichloroacetyl-1-oxa-4-azaspiro[4.5] Decane (AD-67), 4-carboxy-3,4-dihydro-2H-1-benzopyran-4-acetic acid (CL-304415, code number), MON4660 (code number), metcamifen, N1,N2-diallyl-N2-dichloroacetylglycinamide (DKA-24, code number), 1-bromo-4-[(chloromethyl)sulfonyl]benzene (CSB), 2-propenyl 1-oxa-4-azaspiro[4,5]decane-4-carbodithioate (MG-838, code number), 3-(dichloroacetyl)-2,2-dimethyl-1,3-oxazolidine (R-28725, code number), R-29148 (code number), 1-(dichloroacetyl)azepane (TI-35, code number).

[0047] Next, known biopesticides that may be mixed or used in combination are exemplified below, but are not limited to these examples.

[0048] Biopesticide: Haplothrips brevitubus, Franklinothrips vespiformis, Diglyphus isaea, Encarsia formosa, Amblyseius cucumeris, Pseudaphycus malinus, Amblyseius womersleyi, Aphidius colemani, Eretmocerus eremicus, Aphidoletes aphidimyza, Amblyseius swirskii, Orius strigicollis, Phytoseiulus persimilis, Amblyseius degenerans, Phytoseiulus persimilis, Orius sauteri, Dacnusa sibirica, Amblyseius californicus, Chrysoperla nipponensis, Anicetus beneficus.

[0049] Next, known agricultural materials that may be mixed or used in combination are exemplified below, but are not limited to these examples.

[0050] Agricultural materials: Ethylene, hypochlorous acid water (limited to that obtained by electrolyzing an aqueous solution of hydrochloric acid or potassium chloride), sodium bicarbonate, vinegar, humus, humic acid, fulvic acid, seaweed extract, polysaccharide, amino acid, microbial material, functional components derived from animals and plants, microbial metabolites, microbial activation materials, soil spreading agents, soil permeability regulating materials, soil water retention materials, etc., and biostimulants.

[0051] Next, known agricultural fertilizer components that may be mixed or used in combination are exemplified below, but are not limited to these examples.

[0052] Fertilizers include inorganic fertilizers and organic fertilizers. For example, ammonium chloride, ammonium sulfate, ammonium nitrate, ammonium dihydrogen phosphate, ammonium nitrate urea, urea, calcium cyanamide, potassium nitrate, superphosphate of lime, triple superphosphate, potassium dihydrogen phosphate, potassium chloride, potassium sulfate, potassium carbonate, potassium silicate, oil cake, fish meal, rice bran, bat guano, fermented chicken manure, etc. can be mentioned.

[0053] In addition, the plant growth regulator and / or environmental stress tolerance-imparting agent and / or plant disease control agent of the present invention can also be used for plants that have acquired characteristics such as pest resistance, disease resistance, and herbicide resistance by new breeding techniques such as gene recombination and genome editing, and artificial cross-breeding.

[0054] In this way, it is possible to provide a material that contributes to crop production in terms of both the plant growth regulating action of the plant growth regulator containing the viable bacteria or culture of the strain belonging to the genus Bacillus of the present invention as an active ingredient, the increased yield due to imparting resistance to environmental stress, and the suppression of yield reduction due to disease control.

[0055] Hereinafter, examples of the present invention will be described. However, the present invention is not limited only to these examples, and various modifications are possible within the technical idea of the present invention.

Examples

[0056] (Production Example 1: Production of wet bacterial cells) The Bacillus sp. D747 strain (FERM BP-8234) isolated from the air in Shizuoka Prefecture was cultured on a plate medium, and the isolated colonies were inoculated into a flask. After shaking culture at 27°C and 120 rpm for 1 day in 20 ml of bouillon medium (1% meat extract, 1% peptone, 0.5% sodium chloride), the obtained culture solution was added with 1% glucose, 2% soluble starch, 0.5% polypeptone, 1% dry yeast, 1% defatted soybeans, KH 2 PO4 Inoculated into 20 L of a medium with a pH of 6.0 containing 0.2% peptone, 0.2% sodium chloride, and 0.3% calcium carbonate, and subjected to shaking culture at 27 °C and 120 rpm for 3 days. After that, the cells were collected by centrifugation (10,000×g, 15 minutes), suspended in sterile water, and the medium components were washed. This operation was performed twice to obtain wet cells (spore fraction) with a wet weight of about 1 kg. This spore fraction contains 50% by weight of the spores of Bacillus sp. D747 in terms of dry weight.

Example

[0057] (Production Example 2: Production of powdered spores) 1 kg of the spore fraction of Bacillus sp. D747 obtained in Production Example 1 above was suspended in 5 L of distilled water and then treated with a spray dryer (Niro Japan Co., Ltd.) (inlet temperature 150 °C, outlet temperature 100 °C). By crushing the dried product obtained with the spray dryer, about 100 g of dried powdered spores were obtained. This dried powder contains spores of Bacillus sp. D747 at 6×10 11 cfu / g or more.

[0058] Next, representative formulation examples of the agricultural and horticultural composition of the present invention will be given to specifically explain the formulation method. In the following description, “%” indicates weight percentage.

Example

[0059] (Formulation Example 1: Wettable powder) 10% of the powdered spores of Bacillus sp. D747 strain obtained as in Production Example 2 above, 1.5% of sodium naphthalene sulfonate formalin condensate, 1.5% of polyoxyethylene alkyl aryl, 26% of diatomaceous earth, and 61% of clay were uniformly mixed and pulverized to obtain a wettable powder.

Example

[0060] (Growth promotion effect test on potatoes by foliar spraying) The 500-fold aqueous dilution of the wettable powder of Bacillus sp. D747 strain prepared according to Example 3 was sprayed onto the foliage of potato (cultivar: Nishiyutaka) at the 7-compound leaf stage grown in a pot in three sufficient amounts at one-week intervals. The same amount of water was sprayed onto the foliage of the control potato (control plot).

[0061] One week after the third spraying, the above-ground fresh weight of each potato was measured. Also, after holding the underground part in a dryer set at 120 °C for 3 days, it was measured and taken as the underground dry weight. The weight ratio to the control plot was calculated according to Equation (1) below.

[0062] (Equation (1)) Weight ratio to the control plot = (weight of the treated plot / weight of the control plot) × 100

[0063] The results of this test are shown in Table 1 and Figure 1 below. In the control plot, the above-ground fresh weight was 19.2 g / plant and the underground dry weight was 1.17 g / plant, while in the wettable powder-treated plot, the above-ground fresh weight was 20.0 g / plant and the underground dry weight was 1.68 g / plant, both showing higher values than those in the control plot for both the above-ground fresh weight and the underground dry weight. The wettable powder of Bacillus sp. D747 strain showed a growth-promoting effect on potato.

[0064]

Table 1

Example

[0065] (Test on the growth-promoting effect on soybean by irrigation treatment) Soybean (cultivar: Fukuyutaka) was grown in a 128-cell tray filled with nursery soil (registered trademark, manufactured by Jaycam Agri Co., Ltd.) until the first trifoliate leaf unfolded. Then, 2.6 ml per plant of the 500-fold aqueous dilution of the wettable powder prepared according to Example 3 was irrigated at the base of the soybean plant. The same amount of water was irrigated onto the control soybean (control plot). And on the day after the irrigation treatment, it was transplanted into a plastic cup (T-600, manufactured by Tokai Chemical Industry Co., Ltd.) filled with soil.

[0066] On the 21st day after transplantation, the above-ground fresh weight of each soybean plant was measured. Also, the underground part was placed in a dryer set at 120°C for 2 days and then measured to obtain the dry weight of the underground part. The ratio of weight to the control plot was calculated according to Equation 1.

[0067] The test results are shown in Table 2 and Figure 2 below. In the control plot, the above-ground fresh weight was 15.96 g / plant and the dry weight of the underground part was 0.89 g / plant. On the other hand, in the wettable powder treatment plot, the above-ground fresh weight was 17.21 g / plant and the dry weight of the underground part was 1.03 g / plant, both of which were higher than those in the control. The wettable powder of Bacillus sp. D747 strain showed a growth-promoting effect on soybeans.

[0068]

Table 2

Example

[0069] (Test on the growth-promoting effect on sugar beet by seed treatment) Seeds of sugar beet (variety: Paprika) were treated with 0.1 ml of a 1000-fold aqueous dilution of the wettable powder prepared according to Example 3 per 1 g of seeds and air-dried. The control sugar beet seeds were treated with the same amount of water and air-dried in the same manner. The next day, the sugar beet seeds were sown in plastic cups (T-600, manufactured by Tokai Chemical Industry Co., Ltd.) filled with soil.

[0070] Fifty-two days after sowing, the above-ground fresh weight of the sugar beet was measured. Also, the main root was placed in a dryer set at 120°C for 3 days and then measured to obtain the dry weight of the main root. The ratio of weight to the control plot was calculated according to Equation 1.

[0071] The test results are shown in Table 3 and Figure 3 below. In the control group, the above-ground fresh weight was 7.44 g / plant and the main root dry weight was 0.42 g / plant. On the other hand, in the wettable powder treatment group, the above-ground fresh weight was 8.79 g / plant and the main root dry weight was 0.48 g / plant, both of which were higher than those in the control. The wettable powder of Bacillus sp. D747 strain showed a growth-promoting effect on sugar beet.

[0072]

Table 3

Example

[0073] (Test on the effect of relieving high-temperature stress on sugar beet by irrigation treatment) Sugar beet (variety: Paprika) was grown until the four-leaf stage in sugar beet paper pots (a special bottomless and lidless cylinder with a diameter of 19 mm and a length of 130 mm, and 1,400 cylinders (20 rows × 70 layers) were connected together to form one book) filled with nursery soil (registered trademark, manufactured by Jaycam Agri Co., Ltd.). Then, a 500-fold aqueous dilution of the wettable powder prepared according to Example 3 was irrigated at the base of each sugar beet plant at 0.71 ml per plant. The same amount of water was irrigated to the sugar beet for comparison (control group). The next day after the irrigation treatment, the plants were transplanted into plastic cups (T-600, manufactured by Toei Chemical Industry Co., Ltd.) filled with soil. After transplantation, the plants were grown and managed on an outdoor bench with a roof for 40 days, and then for the next 20 days, the plants in the high-temperature group were grown and managed in a greenhouse, while the plants in the normal temperature group were grown and managed on an outdoor bench with a roof. The temperature for the 20-day period was an average of 27.9 °C, a maximum of 40.7 °C, and a minimum of 16.9 °C in the high-temperature group, and an average of 22.7 °C, a maximum of 35.9 °C, and a minimum of 13.5 °C in the normal temperature group.

[0074] Sixty days after transplantation, the main roots of the sugar beet were crushed and filtered through double-layer gauze, and then the sugar content of the crushed liquid was measured with a digital refractometer. Also, the residue filtered through the gauze and the crushed liquid were collected, kept in a dryer set at 120 °C for 4 days, and then weighed to obtain the main root dry weight. The ratio of weight to the control group was calculated according to Equation 1.

[0075] The test results are shown in Table 4 and Figure 4 below. In the control group in the normal temperature zone, the dry weight of the main root was 5.12 g / plant, and the sugar content of the main root was 14.6%. On the other hand, in the control group in the high temperature zone, the dry weight of the main root was 4.14 g / plant, and the sugar content of the main root was 13.3%. Under high temperature conditions, the dry weight of the main root and the sugar content of the main root decreased significantly. In the wettable powder treatment group, the dry weight of the main root in the normal temperature zone was 5.36 g / plant, and the sugar content of the main root was 15.5%. In the high temperature zone, the dry weight of the main root was 4.78 g / plant, and the sugar content of the main root was 14.8%. All showed values higher than the corresponding controls, and the difference was particularly significant in the high temperature zone. Since the wettable powder of Bacillus sp. D747 strain showed a growth promoting effect on sugar beets, and its effect was particularly significant under high temperature conditions, it was considered that the wettable powder of Bacillus sp. D747 strain confers resistance to high temperature stress.

[0076]

Table 4

Example

[0077] (Test on the alleviating effect of water stress on sugar beets by irrigation treatment) Sugar beets (cultivar: Paprika) were grown until the four-leaf stage in paper pots for sugar beets filled with a seedling-growing culture soil (registered trademark, manufactured by Jaycam Agri Co., Ltd.). The paper pots for sugar beets are special bottomless and lidless cylinders, each with a diameter of 19 mm and a length of 130 mm. A total of 1,400 cylinders (20 rows × 70 tiers) are joined together to form one booklet. Then, a 500-fold aqueous dilution of the wettable powder produced according to Example 3 was poured at a rate of 0.71 ml per sugar beet plant at the base of the sugar beet plant. Water of the same amount was poured onto the control sugar beets (control group). On the day after the pouring treatment, the plants were transplanted into plastic cups (T-600, manufactured by Toei Chemical Industry Co., Ltd.) filled with soil. In the water stress group, from the 10th day to the 21st day after transplantation, the plastic cups were placed in a container and flooded with water up to the height of the soil surface. During the other periods, when the soil surface dried out, the bottom of the plastic cup was immersed in water for irrigation. In the normal water management group, throughout the period after transplantation, when the soil surface dried out, the bottom of the plastic cup was immersed in water for irrigation.

[0078] Twenty-eight days after transplantation, the main roots of the sugar beets were placed in a dryer set at 120 °C and held for 3 days, and then weighed to obtain the dry weight of the main roots. The ratio of weight to the control group was calculated according to Equation 1.

[0079] The test results are shown in Table 5 and Figure 5 below. In the control group of the normal water management group, the dry weight of the main roots was 0.70 g / plant. On the other hand, in the control group of the water stress group, the dry weight of the main roots was 0.45 g / plant, and the dry weight of the main roots decreased significantly under water stress conditions. In the wettable powder treatment group, the dry weight of the main roots in the normal water management group was 0.78 g / plant, and the dry weight of the main roots in the water stress group was 0.52 g / plant, both of which were higher than the corresponding controls, and the difference was significant in the water stress group. The wettable powder of Bacillus sp. D747 strain showed a growth-promoting effect on sugar beets, and its effect was particularly significant under water stress conditions. Therefore, it was considered that the wettable powder of Bacillus sp. D747 strain confers tolerance to water stress.

[0080]

Table 5

Example

[0081] (Test on the effect of relieving water stress on cabbages by irrigation treatment) Cabbages (variety: Yui-na) were grown until the two-leaf stage in a 128-hole cell tray filled with nursery soil (registered trademark, manufactured by Jaycam Agri Co., Ltd.). Then, a 500-fold aqueous dilution of the wettable powder produced according to Example 3 was irrigated at the base of each cabbage plant at 2.6 ml per plant. The control cabbages were irrigated with the same amount of water (control group). Then, on the day after the irrigation treatment, they were transplanted into plastic cups (T-600, manufactured by Tokai Chemical Industry Co., Ltd.) filled with soil. In the water stress group, from the 7th day to the 15th day after transplantation, the plastic cups were placed in a container and flooded to the height of the soil surface. During the other periods, when the soil surface was dry, the bottom of the plastic cup was immersed in water for irrigation. In the normal water management group, throughout the period after transplantation, when the soil surface was dry, the bottom of the plastic cup was immersed in water for irrigation.

[0082] Twenty-five days after transplantation, the above-ground fresh weight of the cabbages was measured. Also, the underground part was kept in a dryer set at 120°C for 2 days and then measured to obtain the dry weight of the underground part. The ratio of weight to the control group was calculated according to Equation (1).

[0083] The test results are shown in Table 6 and Figure 6 below. In the control group of the normal water management area, the above-ground fresh weight was 12.81 g / plant, and the below-ground dry weight was 0.40 g / plant. On the other hand, in the control group of the water stress area, the above-ground fresh weight was 10.84 g / plant, and the below-ground dry weight was 0.32 g / plant. Under water stress conditions, the above-ground fresh weight and the below-ground dry weight were significantly reduced. In the wettable powder treatment group, the above-ground fresh weight in the normal water management area was 13.12 g / plant, and the below-ground dry weight was 0.44 g / plant. In the water stress area, the above-ground fresh weight was 12.11 g / plant, and the below-ground dry weight was 0.40 g / plant. All showed values higher than the corresponding controls, and the difference was particularly significant in the water stress area. Therefore, the wettable powder of Bacillus sp. D747 strain showed a growth-promoting effect on cabbages, and its effect was particularly significant under water stress conditions. The wettable powder of Bacillus sp. D747 strain was considered to confer tolerance to water stress.

[0084]

Table 6

[0085] The present invention is summarized as follows.

[0086] An object of the present invention is to provide a natural-derived and safely usable microbial material that can contribute to the improvement of agricultural production by adjusting the growth of crops, increasing the yield by conferring tolerance to environmental stress, and controlling plant diseases at the same time.

[0087] By using viable cells of a specific strain belonging to the genus Bacillus or a culture containing the viable cells as an active ingredient, a plant growth regulator and / or a plant disease control agent that can adjust the growth of crops and increase the yield by conferring tolerance to environmental stress can be provided.

[0088] Moreover, the present invention discovers and provides a previously unknown finding that viable bacteria or cultures of Bacillus bacteria used for disease control, which were not known in conventional knowledge, have the effect of increasing yields by regulating the growth of crops and additionally conferring resistance to environmental stress, as well as a method for using such an effect.

Deposit Number

[0089] In the present invention, the deposit numbers of the microorganisms for which deposit procedures have been carried out are shown below. (1) Bacillus sp. D747 (FERM BP-8234).

Claims

1. A plant growth regulator characterized by containing, as an active ingredient, viable bacteria or a culture of Bacillus subtilis or Bacillus amyloliquefaciens and having a plant growth promoting action or a growth inhibitory action.

2. A plant growth regulator characterized by containing, as an active ingredient, viable bacteria or a culture of any one or more of Bacillus sp. D747 strain, Bacillus subtilis HAI-0404 strain, Bacillus subtilis Y1336 strain, and Bacillus amyloliquefaciens F727 strain and having a plant growth promoting action or a growth inhibitory action.

3. A plant growth regulator characterized by containing, as an active ingredient, viable bacteria or a culture of Bacillus sp. D747 strain and having a plant growth promoting action or a growth inhibitory action.

4. The agent according to any one of Claims 1 to 3, characterized by further imparting environmental stress tolerance to plants in addition to the plant growth promoting action or the growth inhibitory action.

5. The agent according to any one of Claims 1 to 4, characterized by further having a plant disease control action in addition to the plant growth promoting action or the growth inhibitory action.

6. The agent according to any one of Claims 1 to 5, characterized by being for growth regulation of cruciferous plants (including asteraceae plants).

7. The agent according to any one of Claims 1 to 5, characterized by being for growth regulation of solanaceous plants.

8. The agent according to any one of Claims 1 to 5, characterized by being for growth regulation of leguminous plants.

9. The agent according to any one of Claims 1 to 5, characterized by being for growth regulation of brassicaceous plants.

10. A plant growth regulation method and / or an environmental stress tolerance imparting method and / or a plant disease control method, characterized by including a step of bringing one or more plant growth regulators according to any one of Claims 1 to 9 into contact with a plant body and / or soil (particularly rhizosphere soil).

11. The method according to Claim 10, characterized in that the plant body is a plant seed.

12. A seed treated by the method according to Claim 11.

13. The method according to claim 10, characterized in that the step of contacting with the soil is a perfusion treatment or a mixing treatment of the soil.

14. An agent for imparting environmental stress tolerance to plants, characterized by containing viable bacteria or a culture of Bacillus sp. strain D747 as an active ingredient.

15. The agent according to claim 14, characterized in that the environmental stress of the plant is a high temperature stress.

16. The agent according to claim 14, characterized in that the environmental stress of the plant is a water stress.

17. A method for imparting environmental stress tolerance to plants, characterized by using the agent according to claim 14.

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