Exhaust unit and culture apparatus

Abstract

To provide an exhaust unit that can suppress occlusion of an air filter in an energy-saving manner.SOLUTION: An exhaust unit according to one embodiment of the present invention is connected to a closed-system culture vessel of having a gas discharge port, and comprises: an air filter; a piping in which one end is connected to the gas discharge port, and the other end is connected to the air filter, and which partially has a dense part where the piping is densely arranged; and a support member which supports the piping in the dense part.SELECTED DRAWING: Figure 1

Description

Technical Field

[0001] The present invention relates to an exhaust unit and a culture device.

Background Art

[0002] Conventionally, culture devices for culturing cells, microorganisms, etc. in a closed system are known. Generally, a culture device includes a culture vessel, a supply pipe connected to the culture vessel for supplying oxygen etc. necessary for promoting cell growth, and an exhaust pipe for discharging gases such as carbon dioxide. Further, an air filter is provided in the exhaust pipe, but since the gas discharged from the culture vessel contains moisture, there is a problem that the air filter is covered and blocked by condensed water. In response, studies have been made to prevent the air filter from being blocked by condensed water in the culture device.

[0003] For example, Patent Document 1 discloses a system for condensing moisture in a wet gas stream entering or exiting a bioreactor. In Patent Document 1, the system includes a condensation vessel capable of holding a fluid, includes a thermoelectric module, and is configured such that the thermoelectric module cools at least a part of the outer wall surface of the condensation vessel and helps heat a filter disposed downstream of the condensation vessel.

Prior Art Documents

Patent Documents

[0004]

Patent Document 1

Summary of the Invention

Problems to be Solved by the Invention

[0005] However, in such a conventional culture apparatus, in order to suppress the clogging of the air filter by condensed water, the condensation vessel is cooled using a cooling device, and the air filter is heated using a heating device, which increases the power consumption of the culture apparatus.

[0006] In view of the above points, an aspect of the present invention aims to provide an exhaust unit that can suppress clogging of an air filter while saving energy.

Means for Solving the Problems

[0007] An exhaust unit according to an aspect of the present invention is an exhaust unit connected to a closed culture vessel having a gas outlet, and includes an air filter, a pipe having one end connected to the gas outlet and the other end connected to the air filter and having a dense portion densely arranged in a part thereof, and a support member that supports the pipe in the dense portion.

Effects of the Invention

[0008] According to the exhaust unit according to an aspect of the present invention, it is possible to suppress clogging of the air filter while saving energy.

Brief Description of the Drawings

[0009]

Figure 1

Figure 2

Figure 3

Figure 4

Mode for Carrying Out the Invention

[0010] Hereinafter, embodiments for carrying out the present invention will be described with reference to the drawings. For ease of understanding the description, the same reference numerals are given to the same components in each drawing, and redundant descriptions may be omitted. In this specification, "~" indicating a numerical range means including the numerical values described before and after it as the lower limit value and the upper limit value, unless otherwise specified. Also, in this specification, the side of the gas outlet 21 in the culture device 10 is regarded as the upper side, and the side of the shaking device 9 is regarded as the lower side.

[0011] FIG. 1 is a perspective view of a culture device according to an embodiment, FIG. 2 is a view showing a cross-section of the culture device according to an embodiment, and FIG. 3 is a perspective view of an exhaust unit according to an embodiment. As shown in FIGS. 1 to 3, an exhaust unit 1 according to an embodiment of the present invention is an exhaust unit 1 connected to a closed culture vessel 2 having a gas outlet 21. Specifically, the exhaust unit 1 is an exhaust unit 1 connected to a culture vessel 2 for culturing at a temperature higher than room temperature. The exhaust unit 1 has a function of condensing the moisture contained in the gas discharged from the gas outlet 21 of the culture vessel 2 and discharging the gas to the outside.

[0012] As shown in FIGS. 1 and 2, the culture device 10 includes, in addition to the exhaust unit 1, a closed culture vessel 2 having a gas outlet 21.

[0013] The culture vessel 2 is a vessel for enclosing a culture object such as cells or microorganisms to be cultured, a culture medium such as a culture solution for culturing the culture object, and a gas necessary for culturing, and for performing culturing. The culture vessel 2 may be a single-use vessel. The shape of the culture vessel 2 may be, for example, square or circular in plan view.

[0014] The culture container 2 may be a bag-shaped container composed of a flexible packaging material such as a film. Since the exhaust unit 1 has the air filter 3a, the pipe 4, and the support member 5 unitized, even when the culture container 2 is a bag-shaped container, it can be easily attached and detached. Specifically, the culture container 2 is a sealable bag-shaped container and may have a seal portion provided at the peripheral edge. The culture container 2 may be a gusset bag, and in this case, the shape of the culture container 2 may be a cylindrical shape or a prismatic shape.

[0015] From the viewpoint of being able to visually recognize the contents enclosed in the culture container 2 from the outside, it is preferable that part or all of the culture container 2 is transparent. When the culture container 2 is a bag-shaped container, the base material constituting the flexible packaging material is not particularly limited, and examples include polyolefins such as polyethylene, polypropylene, and polystyrene, thermoplastic resins such as polyamide and polyester, or laminates thereof. The culture container 2 may be a laminate in which a biaxially stretched nylon film or a biaxially stretched polyethylene terephthalate (PET) film is laminated as a reinforcing layer on the aforementioned base material. Thereby, the strength of the culture container 2 can be improved. The culture container 2 may be a laminate in which a vapor deposition layer made of silica or alumina, or a resin layer made of all or a partial saponified product of ethylene-vinyl alcohol copolymer or polyvinyl acetate is laminated as a gas barrier layer on the aforementioned base material or reinforcing layer. Thereby, the gas barrier property of the culture container 2 can be improved.

[0016] In order to more strictly prevent leakage of the contents, the culture container 2 may be configured by stacking the flexible packaging material in a double or triple layer. In this case, it is preferable that the inner layer and the outer layer of the flexible packaging material contain a thermoplastic resin. Thereby, by stacking and sealing two or three flexible packaging materials, a culture container 2 which is a bag-shaped container configured by stacking the flexible packaging material in a double or triple layer can be formed.

[0017] The thickness of the base material constituting the culture vessel 2 is not particularly limited and can be appropriately selected according to the material of the base material and the capacity of the culture vessel 2. The thickness of the base material may be, for example, 50 μm to 1000 μm. The capacity of the culture vessel 2 is not particularly limited, but can be, for example, 0.1 L to 1000 L.

[0018] In addition to the gas outlet 21, the culture vessel 2 may have a first gas inlet 22 provided at the upper part and a second gas inlet 23 provided at the lower part. The gas outlet 21 is provided at the upper part of the culture vessel 2. That is, the first gas inlet 22 is provided at a side part or side surface of the culture vessel 2 on the same side as the gas outlet 21. When the culture vessel 2 has an upper surface, a lower surface, and side surfaces, the gas outlet 21 and the first gas inlet 22 may be provided on the upper surface of the culture vessel 2, and the second gas inlet 23 may be provided on the lower surface of the culture vessel 2. The first gas inlet 22 and the second gas inlet 23 are inlets for allowing a gas for performing a target culture, such as oxygen, carbon dioxide, air, an inert gas such as nitrogen or argon, to flow into the culture vessel 2. The first gas inlet 22 is an inlet for allowing a gas to flow into the gas phase in the culture vessel 2, and the second gas inlet 23 is an inlet for allowing a gas to flow into the liquid phase in the culture vessel 2. The gas outlet 21 is an outlet for discharging the supplied gas and gases such as carbon dioxide generated by the culture.

[0019] With this configuration, the culture apparatus 10 can operate in a state of maintaining a closed system until a gas necessary for the culture is introduced into the culture vessel 2 from the first gas inlet 22 and the second gas inlet 23 and the gas generated in the culture vessel 2 is discharged to the outside through the air filter 3a from the gas outlet 21. Therefore, the culture apparatus 10 can suppress clogging of the air filter 3a in an energy-saving manner while maintaining a closed system.

[0020] The gas outlet 21, the first gas inlet 22, and the second gas inlet 23 may be ports. The material constituting the port is not particularly limited, and examples thereof include resin and metal. The port can be inserted into an opening formed in the culture vessel 2, and can be attached to the culture vessel 2 by fixing a flange portion in the shape of a collar to the inner surface of the culture vessel 2 with an adhesive, heat fusion, or the like.

[0021] The exhaust unit 1 includes an air filter 3a, a pipe 4 having one end connected to the gas outlet 21 and the other end connected to the air filter 3a and having a dense portion 41 densely arranged in a part thereof, and a support member 5 that supports the pipe 4 in the dense portion 41. Here, the dense portion 41 means a portion where the distance between the pipes 4 is relatively narrower than other portions when the pipe 4 is bent.

[0022] With this configuration, in the dense portion 41, the surface area of the pipe 4 can be increased. The exhaust unit 1 and the culture device 10 can be naturally cooled by causing the gas discharged from the gas outlet 21 of the culture vessel 2 to flow through the pipe 4 in the dense portion 41, and in the dense portion 41, the moisture contained in the gas can be sufficiently condensed. Therefore, the exhaust unit 1 and the culture device 10 can cool the gas to condense the moisture into condensed water before the gas containing moisture flows through the air filter 3a, and can suppress the moisture from reaching the air filter 3a. Thus, even without heating the air filter 3a, it is possible to prevent the air filter 3a from being covered and blocked by the condensed water.

[0023] As described above, the exhaust unit 1 and the culture device 10 can suppress the blockage of the air filter 3a in an energy-saving manner without using a heating device for heating the air filter 3a or a cooling device for condensing the moisture contained in the gas discharged from the culture vessel 2. Note that natural cooling means lowering the temperature of the gas discharged from the gas outlet 21 of the culture vessel 2 without using a cooling device.

[0024] Since the exhaust unit 1 does not use a cooling device, it is possible to suppress the occurrence of condensation outside the exhaust unit 1. Therefore, even when the exhaust unit 1 is used in a clean room, the cleanliness of the clean room can be maintained.

[0025] In addition, since the exhaust unit 1 is detachable from the gas outlet 21 of the culture vessel 2, in the culture apparatus 10, only the exhaust unit 1 can be replaced with a new one. Furthermore, the user can easily assemble or disassemble the culture apparatus 10 by attaching and detaching it to and from the gas outlet 21 of the culture vessel 2.

[0026] The air filter (exhaust air filter) 3a is provided to make the system a closed system. As the air filter 3a, for example, a filter having a mesh size of 0.05 μm to 0.5 μm can be used.

[0027] The pipe 4 is open to the outside via the air filter 3a and discharges the gas generated by culturing in the culture vessel 2. The material constituting the pipe 4 is not particularly limited, and examples include silicone, thermoplastic elastomer, and metal. The pipe preferably has a variable shape.

[0028] The inner diameter of the pipe 4 is preferably 6 mm to 26 mm, more preferably 7 mm to 24 mm, and even more preferably 8 mm to 22 mm. When the inner diameter of the pipe 4 is 6 mm or more, the exhaust unit 1 and the culture apparatus 10 can suppress the formation of a water film or a region blocked by water, that is, a liquid pool, in the pipe 4 due to the surface tension of the condensed water. In addition, when the inner diameter of the pipe 4 is 26 mm or less, the exhaust unit 1 and the culture apparatus 10 can improve the effect of naturally cooling the gas flowing through the pipe 4.

[0029] The pipe 4 in the dense part 41 is preferably arranged in a spiral shape around the support member 5. Here, the spiral means a three-dimensional curve in a helical shape, including a coil shape, but not including a two-dimensional curve in a spiral shape. Thereby, the exhaust unit 1 and the culture device 10 can suppress the occurrence of liquid accumulation due to condensed water in the pipe 4, and can maintain the cooling effect in the dense part 41. Specifically, the pipe 4 in the dense part 41 is preferably arranged in a spiral shape with the vertical direction as the central axis. Thereby, the pipe 4 in the dense part 41 is inclined with respect to the vertical direction, and the condensed water generated in the pipe 4 naturally flows downward in the vertical direction. Therefore, the exhaust unit 1 and the culture device 10 can more effectively suppress the occurrence of liquid accumulation due to condensed water in the pipe 4, and can more effectively maintain the cooling effect in the dense part 41. Further, the exhaust unit 1 and the culture device 10 can suppress the adhesion of condensed water to the air filter 3a arranged upstream of the dense part 41.

[0030] FIG. 4 is a perspective view showing another example of the exhaust unit according to an embodiment. As shown in FIG. 4, the pipe 4 in the dense part 41 may be arranged in a substantially U-shape continuous on the surface. Here, the substantially U-shape continuous on the surface includes a wave shape having a certain amplitude. In the example shown in FIG. 4, the support member 5 includes a pair of first members 51, a plurality of second members 52 that connect the pair of first members 51 and are arranged in parallel between the pair of first members 51, and a plurality of protrusions 53 provided on each of the pair of first members 51. The pipe 4 is arranged in a substantially U-shape by being fixed to the support member 5 by being hooked on the plurality of protrusions 53. The protrusions 53 may be provided at both ends of each of the plurality of second members 52.

[0031] In addition to the forms shown in FIGS. 3 and 4, the pipe 4 in the dense part 41 may be branched into a plurality of pipes, may be arranged in a meandering shape, or may be arranged in a spiral shape.

[0032] The dense portion 41 is preferably disposed above the gas discharge port 21. Thereby, the condensed water generated in the pipe 4 can flow downward in the vertical direction naturally and can be returned into the culture vessel 2 from the gas discharge port 21. When the pipe 4 in the dense portion 41 is arranged in a spiral shape, it is preferable that the central axis of the spiral coincides with the central axis of the gas discharge port 21. Thereby, the condensed water generated in the pipe 4 can be returned into the culture vessel 2, and the remaining condensed water in the pipe 4 can be suppressed.

[0033] When the pipe 4 in the dense portion 41 is arranged in a spiral shape, the ratio of the length L of the central axis of the spiral to the total length of the pipe 4 is preferably 10:2 to 10:9, more preferably 10:2 to 10:6, and even more preferably 10:2 to 10:3. The total length of the pipe 4 means the length of the entire pipe 4 (from one end to the other end) including the pipe 4 constituting the dense portion 41. When the pipe 4 in the dense portion 41 branches into a plurality of pipes, it means the total length of the pipes 4. Since the ratio of the length L of the central axis of the spiral to the total length of the pipe 4 is 10:2 to 10:9, the surface area of the pipe 4 can be increased more with respect to a certain space. Therefore, the exhaust unit 1 and the culture device 10 can improve the cooling effect on the gas flowing through the pipe 4 without using a cooling device.

[0034] The ratio of the length of the pipe 4 in the dense portion 41 to the total length of the pipe 4 is preferably 100:5 to 100:90, more preferably 100:10 to 100:70, and even more preferably 100:20 to 100:60. Since the ratio of the length of the pipe 4 in the dense portion 41 to the total length of the pipe 4 is 100:5 to 100:90, the surface area of the pipe 4 can be increased more with respect to a certain space. Therefore, the exhaust unit 1 and the culture device 10 can improve the cooling effect on the gas flowing through the pipe 4 without using a cooling device.

[0035] The support member 5 may be anything as long as it can hold the shape or position of the dense portion 41 of the pipe 4, and it is preferably made of a material having a thermal conductivity of 0.2 W / m·K or more. Here, the thermal conductivity means the thermal conductivity measured by a method conforming to JIS H 7801 at 25°C. Thereby, the heat of the gas in the pipe 4 in the dense portion 41 can be dissipated through the support member 5, and the exhaust unit 1 and the culture apparatus 10 can improve the cooling effect in the dense portion 41 without using a cooling device. The thermal conductivity of the material constituting the support member 5 is more preferably 80 W / m·K or more, and even more preferably 200 W / m·K or more.

[0036] The material constituting the support member 5 is not particularly limited, and may be, for example, a metal or a material containing a metal. The material constituting the support member 5 preferably contains aluminum.

[0037] The shape of the support member 5 may be any shape as long as it can hold the shape of the dense portion 41 of the pipe 4 or hold the position of the dense portion 41 of the pipe 4. Examples include a cylindrical shape, a rod shape, a hook shape, a string shape, a net shape, a bag shape, a ladder shape, etc., and these can also be used in combination.

[0038] The shape of the support member 5 is preferably a cylindrical shape or a rod shape. Thereby, since the pipe 4 in the dense portion 41 can be wound around the support member 5 in a spiral shape, the contact area between the support member 5 and the pipe 4 in the dense portion 41 can be increased. Therefore, the exhaust unit 1 and the culture apparatus 10 can dissipate the heat of the gas in the pipe 4 through the support member 5 more, and can improve the cooling effect of the gas flowing through the pipe 4 without using a cooling device.

[0039] Further, the support member 5 may have heat dissipation fins like a heat sink. Thereby, without using power, the support member 5 can be cooled, and the cooling effect of the gas flowing through the pipe 4 can be further improved. When the support member 5 is cylindrical, the heat dissipation fins may be provided inside the cylinder, outside the cylinder, or on both the inside and outside of the cylinder. Further, when the support member 5 is cylindrical, a fan or the like may be used to forcibly ventilate the inside of the cylinder so that air can easily flow through the cylinder. Thereby, the support member 5 can be cooled with less power, and the cooling effect of the gas flowing through the pipe 4 can be further improved.

[0040] When the pipe 4 in the dense part 41 branches into a plurality of pipes, the shape of the support member 5 may be a ring shape capable of bundling the pipes 4.

[0041] The culture device 10 may be used at room temperature (23 to 25 ° C) and may be used by heating the culture vessel 2 to 37 ° C. Thereby, the culture device 10 can suppress the blockage of the air filter 3a with energy saving without using a heating device for heating the air filter 3a or a cooling device for condensing moisture contained in the gas discharged from the culture vessel 2 in a normal culture environment.

[0042] The culture device 10 may include a pipe 7 having one end connected to the first gas inlet 22 and the other end connected to an air filter (inflow air filter) 3b, and a pipe 8 having one end connected to the second gas inlet 23 and the other end connected to an air filter (inflow air filter) 3c. The pipe 7 sends the gas for culturing into the culture vessel 2 through the air filter 3b. The pipe 8 sends the gas for culturing into the culture vessel 2 through the air filter 3c.

[0043] The materials constituting the pipes 7 and 8 are not particularly limited, and examples include polypropylene, silicone, thermoplastic elastomer, metal, etc. The air filters 3b and 3c prevent the entry of microorganisms and the like from the outside into the culture vessel 2. As the air filters 3b and 3c, the same ones as the air filter 3a can be used.

[0044] The membrane area of the air filter 3a may be the same as the membrane area of the air filter 3b or the membrane area of the air filter 3c. Generally, in order to suppress the air filter from being covered and blocked by condensed water, it is also conceivable to make the membrane area of the exhaust air filter connected to the gas outlet of the culture vessel larger than the membrane area of the air filter connected to the gas inlet of the culture vessel. In the exhaust unit 1 and the culture apparatus 10 of the present embodiment, as described above, since the air filter 3a, the pipe 4 having the dense portion 41, and the support member 5 are provided, even if the membrane area of the air filter 3a is the same as the membrane area of the air filter 3b or the membrane area of the air filter 3c, it is possible to suppress the blockage of the air filter 3a with energy saving. The pore diameter of the air filter 3a can be 0.22 μm or less.

[0045] The culture apparatus 10 may be provided with on-off valves provided in the middle of the pipes 7 and 8, respectively. The on-off valve controls the inflow and cutoff of gas into the culture vessel 2 and maintains the culture vessel 2 in a sealed state.

[0046] The culture vessel 2, the pipes 4, 7, 8, and the ports are preferably sterilized. The sterilization means can be selected according to the culture purpose and the like, and examples include radiation such as γ-rays, gases such as ethylene oxide, and heating with steam or the like.

[0047] The culture device 10 may be provided with a power source 91 for vibrating the dense portion 41 and the culture vessel 2. The culture device 10 can culture while stirring the contents 11 in the culture vessel 2, and can return the condensed water generated in the pipe 4 into the culture vessel 2 by the vibration generated when vibrating the culture vessel 2. Therefore, the culture device 10 can suppress the remaining of condensed water in the pipe 4 and the occurrence of liquid accumulation. Specifically, the culture device 10 may include a shaking device 9 having a table portion 92 on which the culture vessel 2 is placed and a power source 91 provided on the table portion 92. As another aspect of the power source 91 shown in FIGS. 1 and 2, the culture device 10 may be provided with a power source 91 that is connected to the dense portion 41 or the support member 5 and vibrates the dense portion 41.

[0048] In the examples shown in FIGS. 1 and 2, the culture vessel 2 is supported by the shaking device 9, but the culture device 10 may include a support member having a bottom surface portion and a peripheral wall portion that supports the culture vessel 2. The culture device 10 may include a support member that supports the exhaust unit 1. Specifically, the culture device 10 may include an air filter 3a or a support member that supports the support member 5.

[0049] (Aspects of the present invention) The present invention includes the following aspects. <Aspect 1> An exhaust unit connected to a closed culture vessel having a gas outlet, an air filter, a pipe having one end connected to the gas outlet, the other end connected to the air filter, and a dense portion densely arranged in a part thereof, and a support member that supports the pipe in the dense portion. <Aspect 2> The exhaust unit according to Aspect 1, wherein the inner diameter of the pipe is 6 mm to 26 mm. <Aspect 3> The exhaust unit according to Aspect 1 or 2, wherein the pipe in the dense portion is arranged in a spiral shape around the support member. <Aspect 4> The support member is made of a material having a thermal conductivity of 0.2 W / m·K or more, and is the exhaust unit according to any one of Aspects 1 to 3. <Aspect 5> The shape of the support member is cylindrical or rod-shaped, and is the exhaust unit according to any one of Aspects 1 to 4. <Aspect 6> The ratio of the length of the central axis of the helix to the total length of the pipe is 10:2 to 10:9, and is the exhaust unit according to any one of Aspects 1 to 5. <Aspect 7> A closed culture vessel having a gas outlet, and an exhaust unit connected to the culture vessel, The exhaust unit includes an air filter, a pipe having one end connected to the gas outlet and the other end connected to the air filter and having a dense portion densely arranged in a part thereof, and a support member that supports the dense portion, and is a culture apparatus. <Aspect 8> The pipe in the dense portion is arranged in a spiral shape with the vertical direction as the central axis, and is the culture apparatus according to Aspect 7. <Aspect 9> The dense portion is arranged above the gas outlet, and is the culture apparatus according to Aspect 7 or 8. <Aspect 10> The culture apparatus according to any one of Aspects 7 to 9 includes a power source for shaking the dense portion and the culture vessel. <Aspect 11> The culture vessel has a first gas inlet provided at the upper part and a second gas inlet provided at the lower part, and is the culture apparatus according to any one of Aspects 7 to 10. <Aspect 12> The culture vessel is a bag-shaped vessel, and is the culture apparatus according to any one of Aspects 7 to 11.

[0050] As described above, the embodiments have been described. However, the above embodiments are presented as examples, and the present invention is not limited by the above embodiments. The above embodiments can be implemented in various other forms, and various combinations, omissions, replacements, changes, etc. can be made without departing from the gist of the invention. These embodiments and their modifications are included in the scope and gist of the invention, and are included in the invention described in the claims and the equivalent scope thereof.

Explanation of Reference Numerals

[0051] 1 Exhaust unit 2 Culture vessel 21 Gas outlet 22 First gas inlet 23 Second gas inlet 3a, 3b, 3c Air filter 4, 7, 8 Pipe 41 Dense part 5 Support member 9 Shaking device 91 Power source 92 Base 10 Culture device

Claims

1. An exhaust unit connected to a closed culture vessel having a gas outlet, an air filter, a pipe having one end connected to the gas outlet, the other end connected to the air filter, and having a dense portion densely arranged in part, and a support member that supports the pipe in the dense portion. An exhaust unit comprising.

2. The exhaust unit according to claim 1, wherein the inner diameter of the pipe is 6 mm to 26 mm.

3. The exhaust unit according to claim 2, wherein the pipe in the dense portion is arranged in a spiral shape around the support member.

4. The exhaust unit according to claim 3, wherein the support member is made of a material having a thermal conductivity of 0.2 W / m·K or more.

5. The exhaust unit according to claim 4, wherein the shape of the support member is cylindrical or rod-shaped.

6. The exhaust unit according to claim 5, wherein the ratio of the length of the central axis of the spiral to the total length of the pipe is 10:2 to 10:

9.

7. A closed culture vessel having a gas outlet, and an exhaust unit connected to the culture vessel, wherein the exhaust unit includes an air filter, a pipe having one end connected to the gas outlet, the other end connected to the air filter, and having a dense portion densely arranged in part, and a support member that supports the dense portion. A culture device having.

8. The culture device according to claim 7, wherein the pipe in the dense portion is arranged in a spiral shape with the vertical direction as the central axis.

9. The culture device according to claim 8, wherein the dense portion is arranged above the gas outlet.

10. The culture device according to claim 9, further comprising a power source for vibrating the dense portion and the culture vessel.

11. The culture device according to claim 10, wherein the culture vessel has a first gas inlet provided at the upper part and a second gas inlet provided at the lower part.

12. The culture device according to claim 11, wherein the culture vessel is a bag-shaped vessel.

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