Hair cosmetic composition

Abstract

To provide a composition that enables effective use and added-value generation of pomegranate seeds that are discarded.SOLUTION: According to the present invention, a hair cosmetic composition includes anhydrous ethanol, menthol, a nonionic surfactant, a modifying agent, and pomegranate seed extract. In a preferred embodiment of the inventive hair cosmetic composition, the modifying agent is at least one selected from disodium edetate, trisodium edetate, and tetrasodium edetate.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present invention relates to a hair cosmetic composition, and more particularly to a hair cosmetic composition containing pomegranate seed extract. [Background technology]

[0002] Pomegranate seeds make up a significant proportion of the fruit, but as a rule, the seeds remaining after juice extraction are discarded. A few examples of pomegranate seed-based supplements include a supplement recommendation system using pomegranate seed extract, in which a suggested supplement calculation unit outputs, when the desired information is beauty, at least one of the following suggested supplements: multivitamins, multiminerals, vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin C, vitamin E, vitamin P, niacin, biotin, collagen, elastin, polyphenols, pantothenic acid, hyaluronic acid, acetylglucosamine, inositol, coenzyme Q10, alpha-carotene, beta-carotene, soybean saponin, royal jelly, twintose, folic acid, rose petal extract, pomegranate seed extract, black cohosh extract, soybean isoflavones, kudzu isoflavone squalene, brewer's yeast, fenugreek extract, calcium, magnesium, copper, iron, zinc, and manganese (Patent Document 1).

[0003] Furthermore, as a composition for improving hair or scalp conditions to prevent hair loss and promote hair growth, a hair or scalp cosmetic composition is known which contains, as active ingredients, i) zinc pyrithione, ii) panthenol, and iii) one or more selected from the group consisting of salicylic acid, nicotinamide, and Thujae Semen extract, and which has the effect of preventing hair loss and promoting hair growth (Patent Document 2). [Prior art documents] [Patent documents]

[0004] [Patent Document 1] Patent Publication No. 2011-232989 [Patent Document 2] Patent Publication No. 2011-178737 DISCLOSURE OF THE INVENTION [Problem to be solved by the invention]

[0005] However, currently, pomegranate seeds are generally disposed of as waste and are not effectively utilized except as described in the above patent documents. Meanwhile, effective utilization of waste materials is being reconsidered. Under these circumstances, it would be more beneficial if waste pomegranate seeds could be effective against various diseases.

[0006] Therefore, an object of the present invention is to provide a composition that can effectively utilize and add value to discarded pomegranate seeds. [Means for solving the problem]

[0007] In order to achieve the above object, the present inventors conducted extensive research into effective utilization of pomegranate seeds, and as a result, came up with the present invention.

[0008] That is, the hair cosmetic composition of the present invention is characterized by containing absolute ethanol, menthol, a nonionic surfactant, a modifier, and pomegranate seed extract.

[0009] In a preferred embodiment of the hair cosmetic composition of the present invention, the modifying agent is at least one selected from disodium edetate, trisodium edetate, and tetrasodium edetate.

[0010] In a preferred embodiment of the hair cosmetic composition of the present invention, the content of the absolute ethanol is 45 to 55% by mass based on the total mass of the composition.

[0011] In addition, in a preferred embodiment of the hair cosmetic composition of the present invention, the nonionic surfactant is at least one selected from polyoxyethylene hydrogenated castor oil, polyoxyethylene sorbitan fatty acid ester, polyoxyethylene sorbitan tetraoleate, polyoxyethylene alkyl ether, alkyl alkanolamide, and polyglycerin fatty acid ester.

[0012] In a preferred embodiment of the hair cosmetic composition of the present invention, the composition further comprises a hair growth agent.

[0013] In addition, in a preferred embodiment of the hair cosmetic composition of the present invention, the hair growth agent is at least one selected from Swertia japonica extract, dipotassium glycyrrhizinate, carrot extract, β-glycyrrhetinic acid, Tamasakizukuri alkaloid, and tocopherol acetate.

[0014] In a preferred embodiment of the hair cosmetic composition of the present invention, the composition further comprises a fragrance. [Effects of the Invention]

[0015] The hair cosmetic composition of the present invention has the advantageous effect of making effective use of pomegranate seeds, which have conventionally been disposed of as waste. In addition to the effects of the original cosmetic composition, the hair cosmetic composition of the present invention has the advantageous effect of improving the healing speed of scalp wounds and the like. [Brief explanation of the drawings]

[0016] [Figure 1]FIG. 1 shows the results of a wound healing test using pomegranate seed extract with a composition according to one embodiment of the present invention. The vertical axis represents the wound area, and the horizontal axis represents time. The results of the wound healing test using a control and various concentrations of PSE (pomegranate seed extract) are shown. Specifically, FIG. 1 shows the wound healing test results and the wound area over time (n=3, mean ± standard error, *p<0.05, **p<0.01 vs. control). [Figure 2] Figure 2 shows a micrograph (magnification: 40x) of a well from a control wound treatment test. The letters a, b, and c in the figure indicate different series. Three wells were prepared per concentration, with the first well (horizontal wound) labeled a, the second well (vertical wound) labeled b, and the third well (diagonal wound) labeled c. [Figure 3] Figure 3 shows a micrograph (magnification: 40x) of a well in a wound healing test using a composition according to one embodiment of the present invention with pomegranate seed extract. Specifically, the micrograph (magnification: 40x) shows a well with 1 μg / mL of PSE (pomegranate seed extract (extract)). The symbols a, b, and c in the figure indicate different series. Three wells were prepared per concentration, with the first well (horizontal wound) labeled a, the second well (vertical wound) labeled b, and the third well (diagonal wound) labeled c. [Figure 4] Figure 4 shows a micrograph (magnification: 40x) of a well in a wound healing test using a composition according to one embodiment of the present invention with pomegranate seed extract. Specifically, the micrograph (magnification: 40x) shows a well with 10 μg / mL of PSE (pomegranate seed extract (extract)). The symbols a, b, and c in the figure indicate different series. Three wells were prepared per concentration, with the first well (horizontal wound) labeled a, the second well (vertical wound) labeled b, and the third well (diagonal wound) labeled c. [Figure 5]Figure 5 shows a micrograph (magnification: 40x) of a well in a wound healing test using a composition according to one embodiment of the present invention with pomegranate seed extract. Specifically, the micrograph (magnification: 40x) shows a well with 50 μg / mL of PSE (pomegranate seed extract (extract)). The symbols a, b, and c in the figure indicate different series. Three wells were prepared per concentration, with the first well (horizontal wound) labeled a, the second well (vertical wound) labeled b, and the third well (diagonal wound) labeled c. [Figure 6] FIG. 6 shows micrographs (magnification: 40x) of wells in a wound healing test using pomegranate seed extract, a control, and a composition according to one embodiment of the present invention. Specifically, micrographs (magnification: 40x) of wells are shown for the control and wells to which 1, 10, and 50 μg / mL of PSE (pomegranate seed extract (extract)) were added, respectively. The elapsed time was 24 hours. The symbols a, b, and c in the figure indicate different series. Three wells were prepared per concentration, with the first well (horizontal wound) labeled a, the second well (vertical wound) labeled b, and the third well (diagonal wound) labeled c. DETAILED DESCRIPTION OF THE INVENTION

[0017] The hair cosmetic composition of the present invention is characterized by containing anhydrous ethanol, menthol, a nonionic surfactant, a modifier, and pomegranate seed extract. In addition to the inherent effects of the cosmetic composition, the present invention can also improve the healing rate of scalp wounds. The pomegranate seed extract is an extract derived from pomegranate seeds. The pomegranate seed extract applicable to the present invention covers all pomegranate seed extracts as long as they are derived from pomegranate seeds. Furthermore, since the composition of the present invention contains pomegranate seed extract, it also has the wound healing effect, which is one of the unique effects of pomegranate seed extract. Therefore, it can also be used as a cosmetic composition for wounds.

[0018] In a preferred embodiment of the hair cosmetic composition of the present invention, the modifier is at least one selected from disodium edetate, trisodium edetate, and tetrasodium edetate from the viewpoint of preventing precipitation and maintaining transparency. The amount of the modifier is not particularly limited, but from the viewpoint of low irritation, it is preferably 0.05 to 0.2 mass%, more preferably 0.01 to 0.5 mass%, relative to the total amount of the composition.

[0019] In a preferred embodiment of the hair cosmetic composition of the present invention, the content of the anhydrous ethanol is preferably 20 to 70% by mass, more preferably 40 to 60% by mass, and even more preferably 45 to 55% by mass, based on the total mass of the composition, from the viewpoint of stabilizing the composition.

[0020] In a preferred embodiment of the hair cosmetic composition of the present invention, the nonionic surfactant is at least one selected from the group consisting of polyoxyethylene hydrogenated castor oil, polyoxyethylene sorbitan fatty acid ester, polyoxyethylene sorbitan tetraoleate, polyoxyethylene alkyl ether, alkyl alkanolamide, and polyglycerin fatty acid ester, from the viewpoint of solubilizing the fragrance. The amount of the nonionic surfactant is not particularly limited, but from the viewpoint of stickiness and foaming of the composition, it is preferably 0.1 to 4.0 mass%, more preferably 0.2 to 2.0 mass%, of the total amount of the composition.

[0021] In a preferred embodiment of the hair cosmetic composition of the present invention, the composition further comprises a hair growth agent from the viewpoint of anti-inflammatory and hair growth effects.

[0022] In a preferred embodiment of the hair cosmetic composition of the present invention, the hair growth agent is at least one selected from Swertia japonica extract, dipotassium glycyrrhizinate, carrot extract, β-glycyrrhetinic acid, Tamasakizukuri alkaloid, and tocopherol acetate, from the viewpoint of anti-inflammatory / anti-inflammatory and hair growth effects. The amount of the hair growth agent is not particularly limited, but from the viewpoint of obtaining a sufficient hair growth effect, it is preferably 0.0001 to 10% by mass, and more preferably 0.001 to 5% by mass, relative to the total amount of the composition.

[0023] In a preferred embodiment of the hair cosmetic composition of the present invention, the composition further comprises a fragrance for the purpose of providing a pleasant fragrance during use. Specifically, the fragrance is at least one selected from essential oils extracted from natural plants and chemically synthesized fragrances. The amount of the fragrance is not particularly limited, but is preferably 0.001 to 2.0% by mass, more preferably 0.01 to 1.0% by mass, relative to the total amount of the composition, from the viewpoints of masking the odor of the raw materials and providing a pleasant fragrance.

[0024] Furthermore, as shown in the Examples below, pomegranate seed extract has been found to have a wound healing effect. However, in the present invention, the term "wound" is not particularly limited. For example, a wound refers to physical damage to surface tissue caused by external or internal factors. Generally, the term "wound" refers to different types of injury, including wounds and cuts. Typically, wounds are also referred to as wounds caused by objects other than the body. Wounds are classified into abrasions, cuts, lacerations, puncture wounds, etc., depending on their shape and cause (mechanism). Compression is typically used to stop bleeding as a first aid treatment for wounds. Wound environment control has been proposed to promote wound recovery, including removal of necrotic tissue, treatment of infection and inflammation, prevention of dryness, and management of exudate. Minor wounds are often washed with tap water or saline, and topical medications and thin wound dressings may be used to maintain an appropriate moist environment.

[0025] Furthermore, examples of methods for preparing pomegranate seeds include the following. Specifically, the pulverized product obtained by pulverizing pomegranate seeds can be immersed in a solvent, such as a polar or nonpolar solvent, such as ethanol, methanol, water, hexane, chloroform, or acetone. Examples of solvents include methanol, ethanol, and water. The pomegranate seed extract can be obtained by immersing the seeds in at least one of these solvents and separating the supernatant. For example, shaking extraction can be performed. In shaking extraction, the seeds can be placed on a rotator in a low-temperature room at, for example, about 4°C and rotated while being extracted. Furthermore, the solvent is characterized by being ethanol, as ethanol extracts have been shown to have high activity.

[0026] The concentration of the pomegranate seed extract is not particularly limited, but is preferably 1 to 100 μg / mL, more preferably 1 to 50 μg / mL, from the viewpoint of not affecting cell viability. For example, as shown in the Examples below, dried pomegranate seeds are powdered in a blender, a 10-fold volume of 100% ethanol is added, and low-temperature shaking extraction is performed at 4°C. After 24 hours, the mixture is centrifuged at room temperature for 20 minutes at 3,000 rpm, and the supernatant is concentrated under reduced pressure to obtain a pomegranate seed ethanol extract (PSE: Punica granatum Seed Ethanol Extract). The extract obtained by vacuum concentration can be weighed and then dissolved in DMSO to adjust the concentration. The extract (both undissolved and dissolved in DMSO) can be stored frozen at a temperature such as -20°C until use in a test.

[0027] Here, the method for preparing pomegranate seed extract will be described in detail. First, pomegranate seeds are prepared. The pomegranate seeds are washed and dried as necessary. It is preferable to dry the seeds thoroughly so that the subsequent pulverization can be performed uniformly.

[0028] Next, the pomegranate seeds are pulverized. The pulverization method is not particularly limited, and known pulverizers such as a ball mill, hammer mill, roller mill, rod mill, sample mill, stamp mill, disintegrator, mortar, and blender with a cooling device can be used. Note that, since heat generated during pulverization may cause decomposition of the pomegranate seed composition, the pulverization time may be set to several seconds, and the process may be repeated about ten times.

[0029] Next, the pomegranate seeds are crushed to obtain a crushed product, which is then immersed in various solvents. The solvent in this case is not particularly limited, and can be appropriately selected depending on the desired effect.

[0030] The immersion can be carried out under gentle stirring. The pulverized material is immersed in various solvents to obtain various solutions. The various solutions are stirred depending on the state of the solution, and in some cases the solution may be left as is. When stirring, the stirring can be continued for 10 to 48 hours, preferably for about one day (24 hours), although there are no particular limitations.

[0031] The supernatant is then separated to obtain pomegranate seed extract. If necessary, the supernatant is evaporated to dryness. Evaporation to dryness can be carried out using an evaporator in a warm bath at 20°C to 60°C, preferably 37°C to 40°C. By evaporating to dryness, the pomegranate seed extract can be stored for a long period of time.

[0032] The components contained in pomegranate seeds are extracted using solvents with different polarities, and are separated based on their physical properties. Therefore, the type and content of the components in the pomegranate seed extract vary depending on the solvent used. [Example]

[0033] An example of the present invention will be described below, but the present invention should not be construed as being limited to the following example.

[0034] Example 1 First, an attempt was made to prepare pomegranate seed extract using ethanol as the solvent. The pomegranate seeds were crushed and subjected to shaking extraction with 100% ethanol. These were concentrated to dryness and redissolved, and used in the experiment as pomegranate seed ethanol extract. Specifically, it was as follows.

[0035] <Preparation of Pomegranate Seed Extract> <Sample> Pomegranate seeds: Obtained from Sunny Place Co., Ltd. <Equipment> Millcer: Manufactured by Iwatsu Sangyo Co., Ltd. Rotary evaporator: Manufactured by EYELA Tokyo Rika Kikai Co., Ltd. Freeze dryer FDU - 1200: Manufactured by EYELA Tokyo Rika Kikai Co., Ltd. <Reagents> 100% Ethanol: Manufactured by Wako Pure Chemical Industries, Ltd. Ethyl acetate: Manufactured by Wako Pure Chemical Industries, Ltd. Hexane: Manufactured by Wako Pure Chemical Industries, Ltd. Methanol: Manufactured by Wako Pure Chemical Industries, Ltd. Silica gel (Wakasil® 60, 64 - 210μm): Manufactured by Wako Pure Chemical Industries, Ltd. MilliQ water <Apparatus> Chromatography column with filter (30φ × 300mm): Manufactured by Cosmos Bead

[0036] <Preparation Method of PSE (Punica granatum Seed Ethanol extract)> The dried pomegranate seeds were powdered with a millcer, 10 times the amount of 100% ethanol (manufactured by Wako Pure Chemical Industries, Ltd.) was added, and low - temperature shaking extraction was carried out at 4°C. After 24 hours, centrifugation was performed at room temperature at 3,000 rpm for 20 minutes, and the supernatant was concentrated under reduced pressure to obtain pomegranate seed ethanol extract (PSE: Punica granatum Seed Ethanol extract).

[0037] All extracts obtained by vacuum concentration were weighed and then dissolved in DMSO (dimethyl sulfoxide) to adjust the concentration (PSE: 50 μg / mL). All extracts (both undissolved and dissolved in DMSO) were stored frozen at -20°C until use in the study.

[0038] <Study on the Skin Functionality of Pomegranate Seed Extract - Scratch Test (Wound Healing)> <List of abbreviations> PSE: Punica granatum Seed Ethanol Extract DMSO: Dimethyl Sulfoxide FBS: Fetal Bovine Serum

[0039] <Experimental Method> <Preculture> -conditions- 6-well plate (total volume per well: 1 mL) Human keratinocyte HaCaT cells used...Cell density at seeding: 1.0 x 10 5 cells / mL ·FBS concentration: 10% Culture medium: D-MEM. Culture until the center of the well becomes confluent (required time: 18 hours), forming a monolayer.

[0040] <Scratch> -Experimental operation- A 10 μL tip was inserted into the end of a Pasteur pipette to create a scratch in the center of the well (wound). (The first series was scratched horizontally, the second series vertically, and the third series diagonally to prevent data confusion.)

[0041] <Main culture> -conditions- ·FBS concentration: 5% PSE concentration: 1, 10, and 50 μg / mL. MEMα medium containing 0.1% DMSO (same concentration as the final concentration of 50 μg / mL) was used as the control (0 μg / mL).

[0042] -Experimental operation- After each wounded well was washed with 1 mL of D-MEM, 500 μL / well of 10% FBS / D-MEM and 2×Final Conc. PSE / D-MEM were added, and culture was initiated.

[0043] <Microscope photography> The start of the culture was set as 0 h, and microscopic photographs (magnification: 40x) were taken at 0, 3, 6, 9, 12, 18, 24, 27, and 33 h.

[0044] <Data processing> The outline of the wound was plotted, and the wound area was calculated using image analysis software. The wound area at 0 hours was set to 100%, and the percentage of the wound area after each time point was compared for each concentration. A state in which the wound area could not be distinguished was considered to be completely healed and recorded as 0%. A t-test was used to test for significance.

[0045] <Experimental Results> The scratch in the control wells took 27 to 33 hours or more to completely heal, whereas the scratch in the PSE-added wells was no longer visible within approximately 24 hours. This suggests an improved healing rate in the PSE-added wells. Furthermore, under these conditions, the effect was greatest with 10 μg / mL, with the area of ​​the scratch at 12 hours being 26.8% (vs. control-0 h) and 8.7% (vs. 10 μg / mL-0 h), demonstrating a 1.2-fold improvement in the healing rate.

[0046] The above results demonstrated that the addition of pomegranate seed extract to culture improved the wound healing rate compared to the control.

[0047] Example 2 In addition, a pomegranate seed extract was also prepared using a different manufacturing method, particularly as a cosmetic raw material (name of pomegranate seed extract: "Pomegranate Seed Extract BG-100").

[0048] <Manufacturing method> 100g of crushed pomegranate seeds are extracted with 1000g of ethanol. After filtering the extract, the solvent is removed by distillation and a 1,3-butylene glycol solution is added. The extract is left to stand in the refrigerator for 1 week to 10 days, and then filtered to obtain the final product. Names and amounts of all cosmetic ingredients (mass%) Pomegranate Seed Extract PUNICA GRANATUM SEED EXTRACT 2.5% Butylene Glycol 97.5%

[0049] The concentration of pomegranate seed extract at this time was 25 μg / mL.

[0050] Example 3 Using the pomegranate seed extract thus obtained, the following cosmetic compositions were attempted to be prepared. The formulation of the composition of the present invention can be applied to hair tonics, hair growth agents, hair care agents, etc. Specifically, attempts were made to prepare cosmetics having the effects related to pomegranate seeds described in the above examples. Various formulations are shown in Table 1.

[0051] [Table 1]

[0052] In Table 1, the products are Emanon CH-40*1: (display name: PEG-40 hydrogenated castor oil), Pomegranate Seed Extract BG-100*2: (display name: Pomegranate Seed Extract, BG), and Swertia japonica Extract Liquid SS*3: (display name: Swertia japonica extract, BG).

[0053] The preparation method is as follows: first, pomegranate seed extract, Swertia japonica extract, dipotassium glycyrrhizinate, and tetrasodium edetate are dissolved in purified water, and then added to a purified water phase in which L-menthol, PEG-40 hydrogenated castor oil, and fragrance have been solubilized in absolute alcohol, and the mixture is stirred. There are several types of sodium salts of edetic acid, and their solubilities in water per 100 mL at 25°C are 11.1 g for disodium edetate, 46.5 g for trisodium edetate, and 60.0 g for tetrasodium edetate, respectively. Since tetrasodium edetate was the highest, tetrasodium edetate was used in this example.

[0054] The purposes of the blended ingredients are as follows: Absolute ethanol: Sterilizing, cooling and menthol solubilizing properties L-menthol: Cooling and soothing effect PEG-40 Hydrogenated Castor Oil - Solubilizes fragrances Fragrance: To add a pleasant fragrance when in use Swertia japonica extract promotes hair growth by promoting blood flow Dipotassium glycyrrhizinate: Anti-inflammatory, anti-inflammatory and hair growth effect Tetrasodium edetate to maintain precipitation and clarity Pomegranate seed extract: Generally said to have female hormone-like effects, it is formulated to improve skin function and wound healing.

[0055] The samples prepared in Examples 1 to 6 were temperature-controlled using a Yamato Scientific Co., Ltd. programmable low-temperature incubator (product name: IN804), and their properties were confirmed at various temperatures below room temperature. The formulations in Table 1 showed high low-temperature stability with 50% absolute alcohol. Stability tests were conducted under specified conditions (25°C, 4.3°C, cycle test between 4.3°C and 43°C, 43°C, freezing and thawing three times, storage in six locations in front of a north-facing window for four weeks).

[0056] In addition, although precipitation may be observed in the formulations of Table 1, it is possible to make the solution transparent by adjusting the amount of nonionic surfactant.

[0057] Furthermore, if transparency is not a concern, cosmetics that provide a silky feel can be produced by blending a pearlizing agent or the like in the opaque state without settling or creaming as in Examples 5 and 6. For shampoos that become cloudy in winter, a pearlizing agent (such as glycol distearate) can be added from the start to create hair shampoos and body soaps with a silky pearly sheen.

[0058] Furthermore, in Examples 1 to 6 in Table 1, the blending amounts of ingredients other than water and alcohol were fixed. In this case, in Examples 4 to 6, water-insoluble ingredients such as fragrances and L-menthol could not be solubilized when the alcohol content was less than 50%, resulting in cloudiness. However, even with the alcohol percentage of Example 4, creaming can be avoided by increasing the amount of solubilizer, such as Emanon CH-40. Conversely, if the amount of water is reduced, edetate tetrasodium becomes difficult to dissolve. Therefore, if transparency is the goal, adjustments can be made taking this into consideration. It was also found that the effect of alcohol is very large.

[0059] In this way, it was found that waste materials could be effectively utilized and scalp cosmetics that have a refreshing feel when used and good stability over time could be obtained. [Industrial Applicability]

[0060] The present invention has a wide range of applications as a hair cosmetic composition that allows for the effective reuse of waste materials, and is highly useful in industry.

Claims

1. A hair cosmetic composition comprising absolute ethanol, menthol, a nonionic surfactant, a modifier, and pomegranate seed extract.

2. 2. The hair cosmetic composition according to claim 1, wherein the modifier is at least one selected from the group consisting of disodium edetate, trisodium edetate, and tetrasodium edetate.

3. 3. The hair cosmetic composition according to claim 1, wherein the content of the anhydrous ethanol is 45 to 55% by mass based on the total mass of the composition.

4. 4. The hair cosmetic composition according to claim 1, wherein the nonionic surfactant is at least one selected from the group consisting of polyoxyethylene hydrogenated castor oil, polyoxyethylene sorbitan fatty acid ester, polyoxyethylene sorbitan tetraoleate, polyoxyethylene alkyl ether, alkyl alkanolamide, and polyglycerin fatty acid ester.

5. The hair cosmetic composition according to any one of claims 1 to 4, further comprising a hair growth agent.

6. 6. The hair cosmetic composition according to claim 5, wherein the hair growth agent is at least one selected from Swertia japonica extract, dipotassium glycyrrhizinate, carrot extract, β-glycyrrhetinic acid, Tamasakizukuri alkaloid, and tocopherol acetate.

7. The hair cosmetic composition according to any one of claims 1 to 6, further comprising a fragrance.

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