Useful microorganism material and method for preventing fouling in drainage system using the same

Abstract

To provide a useful microorganism material that prevents fouling in a drainage system and further removes malodor by utilizing bacteria that have not traditionally employed for cleaning or preventing fouling in drainage systems, and also to provide a method for preventing fouling in a drainage system using the same.SOLUTION: The useful microorganism material of the present invention contains, as main components: Agromyces mediolanus deposited under the name "Isolated Strain a" with accession number NITE P-03967 at NITE Patent Microorganisms Depositary (NPMD); Brucella tritici deposited under the name "Isolated Strain b" with accession number NITE P-03968; and Bosea sp. deposited under the name "Isolated Strain c" with accession number NITE P-03969.SELECTED DRAWING: None

Description

Technical field to which the invention belongs

[0001] The present invention relates to a useful microbial material and a method for preventing fouling in drainage systems using the same. [Background technology]

[0002] Typical stains on drainage systems, particularly toilet bowls, include limescale, yellowing, blackening, and urinary stones (a sparingly soluble calcium compound formed when calcium ions in urine react with phosphoric acid and carbon dioxide in the air) that adhere to the bowl. Toilets can be cleaned by sprinkling a neutral toilet detergent on relatively light stains and then removing them with a toilet brush or similar. For stubborn stains, an alkaline detergent containing chlorine bleach is sprinkled on the stain and then removed with a toilet brush or similar. This allows the bleaching agent to break down and remove the yellowing and blackening. Methods for removing urinary stones and other stains that have adhered to the bowl include dissolving them with an acidic detergent and using microorganisms to soften, dissolve, and remove them.

[0003] Cleaning methods using microorganisms include a fast-acting urinary stone dissolving agent (Japanese Patent Laid-Open No. 2-237688), a urinary stone treatment pad (Japanese Patent Laid-Open No. 7-279215), a method for imparting urinary stone adhesion prevention or inhibitory activity to a toilet bowl (Japanese Patent Laid-Open No. 8-260552), a method and detergent for cleaning flush toilets using microorganisms (Japanese Patent Laid-Open No. 10-277587), and a toilet bowl stain and odor removal tool and method for removing toilet bowl stains and odors (Patent No. 3150299: Japanese Patent Laid-Open No. 11-21973). [Prior art documents] [Patent documents]

[0004] [Patent Document 1] Japanese Patent Application Publication No. 2-237688 [Patent Document 2] Japanese Patent Application Publication No. 7-279215 [Patent Document 3] Japanese Patent Application Publication No. 8-260552 [Patent Document 4] Japanese Patent Application Publication No. 10-277587 [Patent Document 5] Japanese Patent Application Publication No. 11-21973 Summary of the Invention [Problem to be solved by the invention]

[0005] The present invention aims to provide a useful microbial material that can prevent drainage system fouling and remove malodors using bacteria that have not previously been used for cleaning or preventing fouling in drainage systems, and a method for preventing drainage system fouling using the same. [Means for solving the problem]

[0006] The above problems can be solved by any one of the following configurations (1) to (16) of the present invention. (1) A useful microbial material containing, as its main components, Agromyces mediolanus, deposited with the National Patent Microorganisms Depositary (NPMD) of the National Institute of Technology and Evaluation under the accession number NITE P-03967 and the name "isolated b," Brucella tritici, deposited with the accession number NITE P-03968 and the name "isolated b," and Bosea sp., deposited with the accession number NITE P-03969 and the name "isolated c." (2) The microbial material according to (1) above, wherein the ratio of the amount of each bacterium in a dry state to the total amount of the three types of bacterium is within the following range: Agromyces mediolanus: 25 to 40% by mass, Brucella tritici: 25 to 40% by mass, and Bosea species: 25-40% by mass. (3) The useful microbial material according to (1) above, wherein the bacteria are supported on a carrier suitable for immersion in water. (4) The beneficial microbial material according to (1) or (2) above, which is used as a sewage purification and / or deodorizing agent. (5) The beneficial microbial material according to (1) or (2) above, which is used as an agent for removing malodors, reducing ammonia and / or removing urinary stones from toilets. (6) The useful microbial material according to (1) or (2) above, which is used as a purifying agent for cooling water in a cooling tower. (7) The useful microbial material according to (1) or (2) above, which is used as a disinfectant or sterilizer for Legionella bacteria in water sources. (8) The beneficial microbial material according to (1) or (2) above, which is used as an odor remover and / or antibacterial agent in pig farms, poultry farms, cattle sheds and / or horse stables. (9) The useful microbial material according to (1) or (2) above, which is used as an odor remover and / or antibacterial agent for livestock including poultry. (10) The useful microbial material according to (1) or (2) above, which is used as a cutting oil malodor remover in a processing factory. (11) A method for preventing fouling in a drainage system, characterized by contacting the water in the drainage system with the useful microbial material (1) or (2) at least at one point in the drainage system, thereby preventing fouling from adhering to the drainage system or decomposing and removing any adhering fouling. (12) The method for preventing fouling in a drainage system according to (11) above, wherein the useful microbial material is supported on a carrier suitable for immersion in water. (13) The method for preventing soiling of a drainage system according to (11), wherein the drainage system is a drainage system including a toilet bowl and a drain pipe in a flush toilet. (14) The method for preventing fouling of a drainage system according to (11) above, wherein the fouling is urinary stones. (15) The method for preventing soiling of a drainage system according to (11), wherein the beneficial microbial material according to (1) or (2) is supplied to the water in the water supply channel of the flush toilet, thereby substantially bringing the material into contact with the water in the toilet bowl and the drainage pipe. (16) A method for preventing contamination of a cooling tower, comprising immersing the microbial material of (1) or (2) in water, which is the heat medium of the cooling tower, to purify the water, thereby performing maintenance on the cooling tower. In (2) above, the ratios of the bacterial groups that actually work were set to Agromyces mediolanus: 25-40% by mass, Brucella tritici: 25-40% by mass, and Bosea species: 25-40% by mass. However, to pre-load the bacteria onto a carrier, it is more versatile to set the above ratios at an equal 1:1:1 ratio. [Effects of the Invention]

[0007] The useful microbial material according to the present invention can be used in a wide range of applications, including purifying wastewater from drainage systems, equipment used for rearing and breeding, and purifying, sterilizing, and deodorizing livestock and poultry themselves. [Brief explanation of the drawings]

[0008] [Figure 1] FIG. 1 is a schematic diagram of a test biofilter using a useful microbial material according to an embodiment of the present invention. [Figure 2] FIG. 2 is a block diagram showing an example in which the biofilter shown in FIG. 1 is incorporated into a biogas plant. [Figure 3] FIG. 3 is a photograph showing an example of the morphology of isolated bacterium a contained in the useful microbial material of the present invention. [Figure 4] FIG. 4 is a photograph showing an example of the morphology of isolated bacterium contained in the useful microbial material of the present invention. [Figure 5] FIG. 5 is a photograph showing an example of the morphology of isolated bacterium c contained in the useful microbial material of the present invention. [Figure 6] 10 is a photograph showing the action and effect when a useful microorganism material according to an embodiment of the present invention is applied to a urinal. [Figure 7] 10 is a photograph showing the action and effect when the useful microorganism material according to the embodiment of the present invention is applied to another urinal. [Figure 8] 10 is a photograph showing the action and effect when the useful microorganism material according to the embodiment of the present invention is further applied to another urinal. [Figure 9] 10 is a photograph showing the action and effect when the useful microorganism material according to the embodiment of the present invention is further applied to another urinal. [Figure 10] FIG. 10 is a graph showing the change in odor over time when the useful microorganism material according to the embodiment of the present invention is further applied to another urinal. MODE FOR CARRYING OUT THE INVENTION

[0009] Specific configurations of the present invention will be described in detail below. The useful microbial material of the present invention contains, as its main components, Agromyces mediolanus, deposited with the National Patent Microorganisms Depositary (NPMD) of the National Institute of Technology and Evaluation under the accession number NITE P-03967 and the name "isolate a," Brucella tritici, deposited with the accession number NITE P-03968 and the name "isolate b," and Bosea sp., deposited with the accession number NITE P-03969 and the name "isolate c."

[0010] The applicant collected a predetermined amount of good field soil from Edogawa Ward, cultured a group of microorganisms in a medium from the field soil, and requested the Food Analysis Center, a general incorporated foundation, to conduct a microbial identification test using the medium as a sample.

[0011] The following is an excerpt from the center's test report: Test purpose The microorganisms growing on the specimen (hereinafter referred to as "test medium") are identified. Exam Overview 1) Microbial isolation Colonies growing predominantly on the test medium were randomly picked up, inoculated onto agar plates, and cultured, after which the number of species was confirmed. 2) Genus and species identification The bacteria isolated in 1) (hereinafter referred to as "isolated bacteria") were subjected to morphological observation and physiological property tests, and their base sequences were analyzed (Table 1). Based on the results of the base sequence analysis, bacterial species with a homology of 98.7% or more were identified. If multiple bacterial species were identified as candidates, property tests were conducted with reference to the literature, and those with a match to the typical properties were identified.

[0012] Table-1 JPEG2025136228000001.jpg25196

[0013] Test results 1) Microbial isolation As a result of the culture test, the growth of three predominant types of bacteria was observed, which were designated as isolates a to c. 2) Genus and species identification The results of the identification of the genus and species of the isolated bacteria are shown in Table 2. In addition, the results of the morphological observation and physiological property test of the isolated bacteria are shown in Table 3, the results of the homology search by base sequence analysis are shown in Tables 4 to 6, and examples of morphology under microscope observation are shown in Photos 1 to 3 in Figures 3 to 5.

[0014] Table-2 JPEG2025136228000002.jpg2671Genus and species identification results Isolated bacterium Agromyces sp. Due to atypical characteristics (motility), the species could not be determined. Isolate b Brucella tritici Due to differences in characteristics, it was determined to be one species. The isolated bacteria were thought to be either Bosea thiooxidans, B. lupini, B. eneae, or B. vestrisii.

[0015] Table-3 JPEG2025136228000003.jpg194168Characteristics of isolated bacteria

[0016] Table-4 JPEG2025136228000004.jpg6560 Homology search results for isolate a

[0017] Table-5 JPEG2025136228000005.jpg6650 Homology search results for isolate b

[0018] Table-6 JPEG2025136228000006.jpg221165 Homology search results for isolate c

[0019] Agromyces is an actinomycete belonging to the family Microbacteriaceae, and is known to be isolated mainly from soil. Brucella is a non-fermentative, Gram-negative bacillus known primarily as a clinical isolate. Some species or strains can cause brucellosis, and are known to be zoonotic. B. tritici is a species that was transferred from Ochrobactrium to Brucella by Hördt et al. in 2020. This species has also been isolated from soil, plant roots, industrial environments, and clinical sites. Bosea is a motile, non-fermentative, Gram-negative bacillus that has been isolated from soil and root nodules. B. thiooxidans has been isolated from soil and sediments. B. lupini has been isolated from soil and root nodules. B. eneae and B. vestrisii have been isolated from hospital water supplies.

[0020] ·References, Zurdo-piñeiro, JL et al.:Int. J. Syst. Evol. Microbiol.,57,784-788(2007). Stackebrandt, E. and Ebers, J.: microbipolgy today, 152-155(2006). Hördt, A. et al.: Front. Microbiol.,11,468(2020).

[0021] The safety of all three types of bacteria mentioned above has been confirmed by the Center as follows: food sanitation law This genus of bacteria does not fall under the category of food poisoning agents listed in the Food Sanitation Law Enforcement Regulations and the Guidelines for Compiling Food Poisoning Statistics. Infectious disease law This genus does not fall under the category of specific pathogens (types 1 to 4) defined by the Infectious Diseases Act (law on the prevention of infectious diseases and medical care for patients with infectious diseases). (3 References 1) Ministry of Health, Labour and Welfare: Regarding the enforcement of the Ministerial Ordinance amending the Food Sanitation Law Enforcement Regulations, Hygiene No. 1836, December 28, 1999 2) Ministry of Health, Labour and Welfare: "Comparison table of pathogen names and disease names" (2015) 3) National Institute of Infectious Diseases: "National Institute of Infectious Diseases Pathogen Safety Management Regulations Supplement 1" "BSL Classification of Pathogens, etc." (2010). 4) Japanese Society for Bacteriology website (online), available at (http: / / jsbac.org / ), (Referenced 2020-11-26)

[0022] The useful microbial material according to an embodiment of the present invention contains, as its main components, the three types of microorganisms mentioned above: Agromyces mediolanus, deposited with the National Patent Microorganisms Depositary (NPMD) of the National Institute of Technology and Evaluation under the accession number NITE P-03967 and the name "isolate a"; Brucella tritici, deposited with the accession number NITE P-03968 and the name "isolate b"; and Bosea sp., deposited with the accession number NITE P-03969 and the name "isolate c."

[0023] Agromyces mediolanus is a species in the genus Agromyces, which is a genera in the family Microbacteriaceae, order Actinomycetales, and order Actinomycetales.

[0024] Below, we will explain the mycological properties of isolated bacterium a, which is Agromyces mediolanus (see also Table 3 above).

[0025] Taxonomic position (scientific name): Agromyces mediolonus Chemical properties (morphology, growth characteristics, physiological characteristics, etc.): Morphology rod Gram staining + Spores - Motility + Oxidase - Catalase + OF (oxidation-fermentation) not performed Attitude towards oxygen Aerobic Village color: Yellow Urease Not performed β-galactosidase Not performed α-Glucosidase Not performed Citric acid availability Not implemented Place of Origin: Edogawa Ward, Tokyo Source of isolation: Field soil Collection year: July 2013

[0026] Culture conditions Medium name, medium number, etc.: Tryptophan agar (Eiken Chemical) Medium composition: Tryptosoy agar medium 40g Composition (per 1,000 mL of medium) Casein peptone: 15.0g Soy peptone: 5.0g Sodium chloride: 5.0g Agar: 15.0g pH7.3±0.2 Purified water 1000ml Medium pH (before sterilization): Sterilization temperature / time: 121℃ 15 minutes Culture temperature: 30℃ Culture period: 3 days Culture method: ■Aerobic ■ Shaking culture

[0027] Brucella tritici is a species in the genus Brucella, which is a group of Gram-negative, non-spore-forming, aerobic, non-motile cocci or bacilli belonging to the family Brucellaceae.

[0028] Below, we will explain the mycological properties of isolate b, which is Brucella tritici.

[0029] Taxonomic position (scientific name): Brucella tritici Chemical properties (morphology, growth characteristics, physiological characteristics, etc.): Morphology Rod ~ short rod Gram staining - Spores - Motility + Oxidase + Catalase + OF (oxidation-fermentation) Non-decomposition Attitude towards oxygen Aerobic Colony color NP: No characteristic colony pigment produced Urease + β-galactosidase - α-glucosidase - Citric acid availability -

[0030] Place of Origin: Edogawa Ward, Tokyo Source of isolation: Field soil Collection year: July 2013

[0031] Culture conditions Medium name, medium number, etc.: Tryptophan agar medium (Eiken Chemical) Medium composition: Tryptophan agar 40g: see above Purified water 1000ml Medium pH (before sterilization): Sterilization temperature / time: 121℃ 15 minutes Culture temperature: 30℃ Culture period: 3 days Culture method: ■Aerobic ■ Shaking culture

[0032] Bosea sp. is a motile, non-fermentative, Gram-negative bacillus, which has been isolated from soil and root nodules.

[0033] Below, we will explain the mycological properties of isolated bacterium c, which is a Bosea species.

[0034] Taxonomic position (scientific name): Bosea sp. Chemical properties (morphology, growth characteristics, physiological characteristics, etc.): Morphology Rod ~ short rod Gram staining - Spores - Motility + Oxidase + Catalase + OF (oxidation-fermentation) Non-decomposition Attitude towards oxygen Aerobic Colony color NP: No characteristic colony pigment produced Urease Not performed β-galactosidase Not performed α-Glucosidase Not performed Citric acid availability Not implemented

[0035] Place of Origin: Edogawa Ward, Tokyo Source of isolation: Field soil Collection year: July 2013

[0036] Culture conditions Medium name, medium number, etc.: Tryptophan agar medium (Eiken Chemical) Medium composition: Tryptophan agar 40g: see above Purified water 1000ml Medium pH (before sterilization): Sterilization temperature / time: 121℃ 15 minutes Culture temperature: 30℃ Culture period: 3 days Culture method: ■Aerobic ■ Shaking culture

[0037] In the useful microbial material according to the embodiment of the present invention, the above three types of bacteria are preferably immobilized on a carrier to make handling easier.

[0038] The quantitative ratio of the three types of bacteria contained in the carrier-immobilized microorganisms is not particularly limited. That is, when the various types of bacteria are allowed to coexist in water, they will be stable at a roughly constant ratio depending on the storage or culture conditions. However, if the storage or culture conditions are appropriately set so that the quantitative ratios shown below are achieved, the functions of preventing and purifying the wastewater system and removing (preventing) bad odors can be extremely well achieved.

[0039] Agromyces mediolanus: 25 to 40% by mass, Brucella tritici: 25 to 40% by mass, and Bosea species: 25-40% by mass. In the above, the ratios of the bacterial groups that actually work are Agromyces mediolanus: 25-40% by mass, Brucella tritici: 25-40% by mass, and Bosea species: 25-40% by mass. However, to pre-load the bacteria onto a carrier, it is more versatile and convenient to use an equal ratio of 1:1:1.

[0040] The carrier for immobilizing the microorganisms preferably has a large capacity for holding the microorganisms and is easy to activate the microorganisms.

[0041] Suitable carriers include rocks (e.g., perlite and diatomaceous earth) or crushed rocks, gravel, sand, plastics, ceramics (e.g., alumina, silica, natural zeolite, synthetic zeolite), and talc. Porous materials with continuous pores, such as porous ceramics and porous plastics, are particularly preferred. For example, fine powder (trade name: perlite) made by heat-treating crushed perlite at high temperature and pressure and then rapidly reducing the pressure to make it porous is suitable because of its ability to support the growth of fungi. In such porous materials, the diameter of the pores is preferably approximately 2 to 10 μm. The carrier may be in any form, such as agglomerates, granules, powder, fine powder, platelets, or needles. However, powders with an average particle size of 2 mm or less, particularly 50 μm to 1 mm, are preferred. If necessary, such carrier-immobilized microorganisms may be stored in a container with good water permeability and breathability, such as cloth or netting.

[0042] In addition to these, ribbon-shaped or sheet-shaped woven or nonwoven fabrics can also be used as the carrier.

[0043] To support the microorganisms on such a carrier, the carrier may be mixed with a dispersion of the microorganisms of the present invention and then dried, but the microorganisms may also be cultured directly on the carrier.

[0044] The amount of microorganisms supported on the carrier varies depending on the conditions for supporting the microorganisms, but is preferably 5 to 20 billion / cm. 3 , preferably 10 to 10 billion particles / cm 3 is.

[0045] The useful microbial material of the present invention may be incorporated into a device such as that shown in the above-mentioned Japanese Patent No. 3150299. The methods described in the above patent publications can be used as they are for supporting microbial communities on the materials that make up these devices. The device can be fabricated by assembling the materials loaded with microorganisms by the above-described method into a plate or ball shape. In the case of plate-shaped and small ball-shaped devices, the knitted fabric forms the outermost layer.

[0046] In order to confirm the effectiveness of this useful microbial material, the useful microbial material was supported on perlite and made into a useful microbial material package 10 measuring 30 cm wide x 30 cm long x 120 cm high (volume: 81 liters), which was then introduced into the test equipment E shown in Figure 1. This test equipment E is equipped with a septic tank 12 incorporating the useful microbial material package 10, a wastewater tank 14 for storing wastewater, a supply means 16 having a pump for supplying wastewater from the wastewater tank 14 to the septic tank 12, and a circulation means 18 for pumping up the wastewater supplied by the supply means 16, purified to a certain extent by the useful microbial material package 10, and accumulated at the bottom of the septic tank 12, and bringing it back into contact with the useful microbial material package 10 in the septic tank 12.

[0047] The wastewater to be treated had a COD of 25,000 to 35,000 mg / L. When the wastewater was treated under the conditions of a wastewater supply rate of 8 liters per hour from the supply means 16, a wastewater supply rate of 180 to 200 liters per hour from the circulation means 18, and ambient temperature: room temperature, the COD fell to about 4,000 to 6,000 mg / L, and purification was confirmed.

[0048] A wastewater purification plant 100 including a biological purification treatment device configured like the test facility E shown in FIG. 1 can be used for purifying wastewater in a biogas plant BG. The wastewater purification plant 100 can be configured, for example, as shown in Figure 2, to include, in order, an aeration pond 102 with an air blower, a first clarifier 104, the above-mentioned biological purification treatment device 106, a second clarifier 108, a polishing pond 110 with an air blower, and a third clarifier 112.

[0049] The useful microbial material of the present invention as described above can be used as a fouling prevention / removal agent for drainage systems. A method for preventing and removing fouling in drainage systems using this fouling prevention / removal agent for drainage systems simply involves contacting or dispersing the bacterial colony of this fouling prevention / removal agent in the water of the drainage system in some way. This purifies the wastewater itself, and the bacterial colony in this wastewater (or supply water) can remove and prevent fouling from the drainage system, as well as eliminate and prevent bad odors.

[0050] For example, in the case where the drainage system includes a flush toilet bowl and a drainage pipe, by placing the above-mentioned stain-preventing agent in the water supply tank of the water supply system, bacteria can be grown in the water supply and then supplied to the drainage system, thereby effectively preventing and removing stains and preventing and removing bad odors.

[0051] The present invention can be effectively applied not only to the drainage system of the toilet described above, but also to other drainage systems in factories and buildings, and drainage systems in kitchens and bathrooms in homes, as long as the wastewater contains calcium and organic matter, and can also be effectively used as a purifying agent for the cooling water of cooling towers. [Example]

[0052] Next, an embodiment of the present invention will be described. First Example This microbial material was placed in the flush water outlets of several urinals in the restrooms of the world's largest furniture retailer in Osaka City. The flush water is automatically released to the urinal after use. This released flush water comes into contact with the microbial material on its way to the bottom of the toilet, becoming flush water containing microorganisms and then supplied to the toilet. The frequency of water supply varied depending on the toilet, but was approximately every 10 minutes to 1 hour. The time-dependent improvement of urinary stones adhering to the inside of the drain pipe of one of the men's urinals is shown in Figure 6 (a), (b), (c), and (d). Figure 6(a) shows the condition on February 19, 2016, before treatment, with yellow or brown urinary stones clinging thickly to the inner walls of the drainpipe. Approximately one month after the installation of the microbial material, on March 24, 2016, the condition changed to Figure 6(b), and about one month after that, on April 25, 2016, the condition changed to Figure 6(c), and about two months after that, on June 20, 2016, the condition changed to Figure 6(d), with the yellow or brown urinary stones gradually breaking down, turning white and soft and being removed. Figure 6(e) shows the condition after cleaning with a brush, etc.

[0053] Second Example The microbial material was placed in the flush water outlets of several men's urinals in a customer restroom (used by approximately 115,000 people per week) on the first floor of a large shopping center in Nagoya City. As in the first example, flush water is automatically supplied to the urinals after use, and microorganisms are present in this flush water. The time course of the improvement of urinary stones adhering to the inside of the drain pipe of one of the men's urinals is shown in Figures 7(a), (b), and (c). Figure 7(a) shows the state before treatment, Figure 7(b) shows the state three months after treatment began, and Figure 7(c) shows the state approximately three months later (approximately six months after treatment began). After the introduction of this microbial material, the urinary stones in the drainage pipe of the men's urinal were removed in about six months.

[0054] Third Example This is an example of the application of the microbial material to a men's urinal in the head office building of one of the applicant's clients. The photographs in Figure 8 show the effect, with (a) showing the condition of the urinal drain trap and drain pipe before application and (b) showing the condition six months after application. Six months after the introduction of this microbial material, the urinary stones in the urinal drain trap and drain pipe had been decomposed by the microbial material and had turned white and swollen. In this state, the urinary stones could be removed simply by scrubbing them lightly with a brush. In addition, the process of applying this microbial material to a men's urinal in another location and then easily removing the softened urinary stones by rubbing them with a toilet brush is shown in Figure 9 (a) to (d).

[0055] Fourth Example The microbial material was placed on the flush water outlets of several men's urinals in the customer restroom (used by approximately 115,000 people per week) on the first floor of the large shopping center in Nagoya City, and the odor values ​​(levels) were measured from July 3, 2013, when the materials were first installed, to February 1, 2014. The results are shown in the graph in Figure 10. The test equipment used was a portable odor sensor (Riken Keiki OD-85), with the reference value (where the air is good) set at 200. The higher the value, the stronger the odor. In Figure 10, line L1 is a line on which the odor values ​​in a toilet room where the present microbial material was installed were plotted on the measurement date, line L2 is a line on which the odor values ​​in the urinal trap of a toilet where the present microbial material was installed were plotted on the measurement date, and lines L3 and L4 are lines on which the odor values ​​in a toilet room in its original state without the present microbial material installed were plotted on the measurement date, and lines on which the odor values ​​in the urinal trap of a toilet where the present microbial material was not installed were plotted on the measurement date, respectively. This graph also clearly shows the effect of the useful microbial material of the present invention. [Explanation of symbols]

[0056] E Testing Equipment 10. Useful Microorganism Materials Package 12 Septic tank 16 Means of supply 18 Circulation means BG Biogas Plant 100 Wastewater purification plant 102 Aeration pond 104 First Purifier 106 Biological purification treatment equipment 108 Second Purifier 110 Polishing Pond 112 Third Purifier

Claims

1. A useful microbial material containing, as its main components, Agromyces mediolanus, deposited with the National Patent Microorganisms Depositary (NPMD) of the National Institute of Technology and Evaluation under the accession number NITE P-03967 and the name "isolated b," Brucella tritici, deposited with the accession number NITE P-03968 and the name "isolated b," and Bosea sp., deposited with the accession number NITE P-03969 and the name "isolated c."

2. 2. The microbial material according to claim 1, wherein the ratio of the amount of each of the three types of bacteria in the total amount of the three types of bacteria in a dry state is within the following ranges: Agromyces mediolanus: 25 to 40% by mass, Brucella tritici: 25 to 40% by mass, and Bosea species: 25-40% by mass.

3. 2. The useful microbial material according to claim 1, wherein the bacteria are supported on a carrier suitable for immersion in water.

4. 3. The useful microbial material according to claim 1 or 2, which is used as a purifying and / or deodorizing agent for wastewater.

5. 3. The useful microbial material according to claim 1, which is used as an agent for removing malodors, reducing ammonia and / or removing urinary stones from toilets.

6. 3. The useful microbial material according to claim 1, which is used as a purifying agent for cooling water in a cooling tower.

7. 3. The useful microbial material according to claim 1 or 2, which is used as a bactericide or disinfectant for Legionella bacteria in water sources.

8. 3. The useful microbial material according to claim 1, which is used as an odor remover and / or antibacterial agent in pig farms, poultry farms, cattle sheds and / or horse stables.

9. 3. The useful microbial material according to claim 1 or 2, which is used as an odor remover and / or antibacterial agent for livestock including poultry.

10. 3. The useful microbial material according to claim 1, which is used as an agent for removing bad odors from cutting oil in processing plants.

11. A method for preventing fouling in a drainage system, characterized by contacting the microbial material of claim 1 or 2 with the water in the drainage system at at least one point in the system, thereby preventing fouling from adhering to the drainage system or decomposing and removing any fouling that has adhered to the system.

12. 12. The method for preventing fouling in a drainage system according to claim 11, wherein the microbial material is carried on a carrier suitable for immersion in water.

13. The method for preventing soiling of a drainage system according to claim 11, wherein the drainage system is a drainage system including a toilet bowl and a drain pipe in a flush toilet.

14. The method for preventing fouling in a drainage system according to claim 13, wherein the fouling is urinary stones.

15. 13. The method for preventing soiling of a drainage system according to claim 12, wherein the microbial material according to claim 1 or 2 is supplied to the water in the water supply channel of said flush toilet, thereby causing it to substantially come into contact with the water in the toilet bowl and the drainage pipe.

16. A method for preventing contamination of a cooling tower, comprising the steps of: immersing the microbial material of claim 1 or 2 in water, which is a heat medium of the cooling tower, to purify the water, thereby performing maintenance on the cooling tower.

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