Anti-il31 antibodies for veterinary use

Abstract

To provide methods and compounds that can be used specifically to bind interleukin-31 (IL31) in companion animals to treat IL31-induced conditions and reduce IL31 signaling.SOLUTION: Provided are various embodiments relating to anti-IL31 antibodies binding to canine IL31. Such antibodies can be used in methods to treat IL31-induced conditions in companion animals, such as canines, felines, and equines.SELECTED DRAWING: Figure 1A-B

Description

[Technical Field]

[0001] This application claims the benefit of International Application No. PCT / US2017 / 023788, filed March 23, 2017, and U.S. Provisional Patent Application No. 62 / 463,543, filed February 24, 2017, each of which is incorporated herein by reference in its entirety for all purposes.

[0002] The present invention relates to isolated anti-IL31 antibodies that bind, for example, to canine IL31, and methods of using same, for example, to treat IL31-induced conditions or reduce IL31 signaling function in cells in companion animals, such as dogs, cats, and horses. [Background technology]

[0003] Interleukin-31 (IL31) is a cytokine primarily produced by Th2 cells and is understood to be involved in promoting skin diseases such as pruritus and other forms of allergic diseases (e.g., atopic dermatitis). IL31 functions by binding to its receptor to activate downstream activities, such as JAK1 activation, which is thought to cause many of the clinical problems associated with dermatitis and other disorders.

[0004] Companion animals, such as cats, dogs, and horses, suffer from many skin diseases similar to those in humans, such as atopic dermatitis. However, the sequence of IL31 differs between humans, cats, dogs, and horses. Thus, there is a need for methods and compounds that can be used to specifically bind to IL31 in companion animals to treat IL31-induced conditions and reduce IL31 signaling. Summary of the Invention

[0005] In some embodiments, an isolated antibody that binds to canine IL31 is provided. In some embodiments, the antibody binds to an epitope comprising amino acids 34-50 of SEQ ID NO: 22. In some embodiments, the antibody binds to an epitope comprising the amino acid sequence of SEQ ID NO: 23. In some embodiments, the antibody binds to an epitope comprising the amino acid sequence of PSDX1X2KI (SEQ ID NO: 45), where X is any amino acid residue. In some embodiments, X1 is a hydrophobic amino acid. In some embodiments, X1 is selected from A, V, I, and L. In some embodiments, X1 is selected from V and I. In some embodiments, X2 is a hydrophilic amino acid. In some embodiments, X2 is selected from A, R, K, Q, and N. In some embodiments, X2 is selected from R and Q. In some embodiments, X1 is V and X2 is R. In some embodiments, X1 is I and X2 is Q. In some embodiments, the antibody binds to an epitope comprising the amino acid sequence of SEQ ID NO: 88.

[0006] In some embodiments, the antibody has a density of 5×10 as measured by biolayer interferometry. -6 Less than M, 1 x 10 -6 Less than M, 5 x 10 -7 Less than M, 1 x 10 -7 Less than M, 5 x 10 -8 Less than M, 1 x 10 -8 Less than M, 5 x 10 -9 Less than M, 1 x 10 -9 Less than M, 5 x 10 -10 Less than M, 1 x 10 -10 Less than M, 5 x 10 -11 Less than M, 1 x 10 -11 Less than M, 5 x 10 -12 Less than M or 1 x 10 -12 It binds to canine IL31 with a dissociation constant (Kd) of less than M.

[0007] In some embodiments, the antibody reduces IL31 signaling function in the companion animal species as measured by reduced phosphorylation of STAT-3, hi some embodiments, the companion animal species is a dog, cat, or horse.

[0008] In some embodiments, the antibody binds to feline IL31 or equine IL31 as determined by immunoblot analysis and / or biolayer interferometry. In some embodiments, the antibody competes with monoclonal M14 antibody for binding to canine IL31. In some embodiments, the antibody competes with monoclonal M14 antibody for binding to feline IL31. In some embodiments, the antibody does not bind to human IL31 as determined by immunoblot analysis and / or biolayer interferometry.

[0009] In some embodiments, the antibody is a monoclonal antibody. In some embodiments, the antibody is a canine, caninized, feline, felineized, equine, equine, or chimeric antibody. In some embodiments, the antibody is a chimeric antibody comprising a murine variable heavy chain framework region or a murine variable light chain framework region.

[0010] In some embodiments, the antibody comprises a heavy chain and a light chain: a. the heavy chain comprises a CDR-H1 sequence having at least 85% sequence identity, at least 90% sequence identity, at least 95% sequence identity, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO: 1, a CDR-H2 sequence having at least 85% sequence identity, at least 90% sequence identity, at least 95% sequence identity, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO: 2, 62, 89, or 87, and a CDR-H3 sequence having at least 85% sequence identity, at least 90% sequence identity, at least 95% sequence identity, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO: 3; b. The light chain comprises a CDR-L1 sequence having at least 85% sequence identity, at least 90% sequence identity, at least 95% sequence identity, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO:8 or SEQ ID NO:63, a CDR-L2 sequence having at least 85% sequence identity, at least 90% sequence identity, at least 95% sequence identity, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO:9, and a CDR-L3 sequence having at least 85% sequence identity, at least 90% sequence identity, at least 95% sequence identity, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO:10.

[0011] In some embodiments, the antibody comprises a heavy chain comprising (a) a CDR-H1 comprising the amino acid sequence of SEQ ID NO: 1, (b) a CDR-H2 comprising the amino acid sequence of SEQ ID NO: 2 or 89, and (c) a CDR-H3 comprising the amino acid sequence of SEQ ID NO: 3.

[0012] In some embodiments, the antibody comprises a heavy chain comprising (a) a CDR-H1 comprising the amino acid sequence of SEQ ID NO: 1, (b) a CDR-H2 comprising the amino acid sequence of SEQ ID NO: 62 or 87, and (c) a CDR-H3 comprising the amino acid sequence of SEQ ID NO: 3.

[0013] In some embodiments, the antibody comprises a light chain comprising (a) a CDR-L1 comprising the amino acid sequence of SEQ ID NO: 8, (b) a CDR-L2 comprising the amino acid sequence of SEQ ID NO: 9, and (c) a CDR-L3 comprising the amino acid sequence of SEQ ID NO: 10.

[0014] In some embodiments, the antibody comprises a light chain comprising (a) a CDR-L1 comprising the amino acid sequence of SEQ ID NO: 63, (b) a CDR-L2 comprising the amino acid sequence of SEQ ID NO: 9, and (c) a CDR-L3 comprising the amino acid sequence of SEQ ID NO: 10.

[0015] In some embodiments, the antibody comprises one or more of: (a) a variable region heavy chain framework 1 (HC-FR1) sequence of SEQ ID NO: 4, 70, or 79; (b) a HC-FR2 sequence of SEQ ID NO: 5, 71, or 80; (c) a HC-FR3 sequence of SEQ ID NO: 6, 72, 73, or 81; (d) a HC-FR4 sequence of SEQ ID NO: 7, 74, or 82; (e) a variable region light chain framework 1 (LC-FR1) sequence of SEQ ID NO: 11, 75, or 83; (f) a LC-FR2 sequence of SEQ ID NO: 12, 76, or 84; (g) a LC-FR3 sequence of SEQ ID NO: 13, 77, or 85; or (h) a LC-FR4 sequence of SEQ ID NO: 14, 78, or 86.

[0016] In some embodiments, the antibody a. (i) a variable light chain sequence having at least 85%, at least 90%, at least 95%, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO: 24, (ii) a variable heavy chain sequence having at least 85%, at least 90%, at least 95%, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO: 25, or (iii) the variable light chain sequence of (i) and the variable heavy chain sequence of (ii), or b. (i) a variable light chain sequence having at least 85%, at least 90%, at least 95%, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO: 16; (ii) a variable heavy chain sequence having at least 85%, at least 90%, at least 95%, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO: 15; or (iii) the variable light chain sequence of (i) and the variable heavy chain sequence of (ii); or c. (i) a variable light chain sequence having at least 85%, at least 90%, at least 95%, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO: 32; (ii) a variable heavy chain sequence having at least 85%, at least 90%, at least 95%, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO: 33; or (iii) the variable light chain sequence of (i) and the variable heavy chain sequence of (ii). Includes:

[0017] In some embodiments, the antibody comprises the variable light chain sequence of SEQ ID NO: 24, SEQ ID NO: 16, or SEQ ID NO: 32. In some embodiments, the antibody comprises the variable heavy chain sequence of SEQ ID NO: 25, SEQ ID NO: 15, or SEQ ID NO: 33. In some embodiments, the antibody comprises the variable light chain sequence of SEQ ID NO: 24 and the variable heavy chain sequence of SEQ ID NO: 25, the variable light chain sequence of SEQ ID NO: 16 and the variable heavy chain sequence of SEQ ID NO: 15, or the variable light chain sequence of SEQ ID NO: 32 and the variable heavy chain sequence of SEQ ID NO: 33.

[0018] In some embodiments, the antibody is a chimeric antibody that comprises a constant heavy chain region or a constant light chain region derived from a companion animal.

[0019] In some embodiments, the antibody comprises (a) a canine heavy chain constant region selected from an IgG-A, IgG-B, IgG-C, and IgG-D constant region; (b) a feline heavy chain constant region selected from an IgG1, IgG2a, and IgG2b constant region; or (c) an equine heavy chain constant region selected from an IgG1, IgG2, IgG3, IgG4, IgG5, IgG6, and IgG7 constant region.

[0020] In some embodiments, the antibody a. (i) the light chain amino acid sequence of SEQ ID NO: 26, (ii) the heavy chain amino acid sequence of SEQ ID NO: 27, or (iii) the light chain amino acid sequence of (i) and the heavy chain amino acid sequence of (ii), or b. (i) the light chain amino acid sequence of SEQ ID NO: 30, (ii) the heavy chain amino acid sequence of SEQ ID NO: 31, or (iii) the light chain amino acid sequence of (i) and the heavy chain amino acid sequence of (ii), or c. (i) the light chain amino acid sequence of SEQ ID NO: 34, (ii) the heavy chain amino acid sequence of SEQ ID NO: 35, or (iii) the light chain amino acid sequence of (i) and the heavy chain amino acid sequence of (ii). Includes:

[0021] In some embodiments, the antibody comprises the light chain amino acid sequence of SEQ ID NO: 21. In some embodiments, the antibody comprises the heavy chain amino acid sequence of SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, or SEQ ID NO: 20.

[0022] In some embodiments, the antibody is an antibody fragment selected from an Fv, scFv, Fab, Fab', F(ab')2, and Fab'-SH.

[0023] In some embodiments, the antibody is bispecific, wherein the antibody binds to IL31 and one or more antigens selected from IL17, TNFα, CD20, CD19, CD25, IL4, IL13, IL23, IgE, CD11α, IL6R, α4-integrin, IL12, IL1β, or BlyS.

[0024] In some embodiments, provided are isolated nucleic acids encoding the anti-IL31 antibodies described herein above. In some embodiments, provided are host cells comprising nucleic acids encoding the anti-IL31 antibodies described herein above. In some embodiments, provided are methods of producing anti-IL31 antibodies comprising culturing such host cells comprising nucleic acids encoding the anti-IL31 antibodies described herein above and isolating the antibodies.

[0025] In some embodiments, a pharmaceutical composition is provided comprising an anti-IL31 antibody described herein and a pharmaceutically acceptable carrier. In some embodiments, a pharmaceutical composition is provided comprising an anti-IL31 antibody described herein and a pharmaceutically acceptable carrier, wherein the pharmaceutically acceptable carrier comprises L-histidine, sodium chloride, and polysorbate 80.

[0026] In some embodiments, the pharmaceutical composition has a pH of 5.0 to 6.2. In some embodiments, the pharmaceutical composition has a pH of 5.0 to 6.0, or 5.3 to 5.7, or 5.5.

[0027] In some embodiments, the pharmaceutical composition has an L-histidine concentration of 5 mM to 100 mM, 10 mM to 50 mM, 20 mM to 30 mM, 10 to 30 mM, or 20 mM.

[0028] In some embodiments, the pharmaceutical composition has a sodium chloride concentration of 80-200 mM, 100-175 mM, 120-150 mM, or 140 mM.

[0029] In some embodiments, the pharmaceutical composition has a concentration of polysorbate 80 between 0.005 mg / mL and 0.5 mg / mL, between 0.01 mg / mL and 0.1 mg / mL, or 0.05 mg / mL.

[0030] In some embodiments, the pharmaceutically acceptable carrier comprises at least one sugar. In some embodiments, the pharmaceutical composition has a concentration of at least one sugar between 0.5% and 20%, between 1% and 10%, between 1% and 5%, or between 1% and 3%. In some embodiments, the pharmaceutically acceptable carrier comprises sucrose, trehalose, D-mannitol, maltose, and / or sorbitol.

[0031] In some embodiments, the pharmaceutically acceptable carrier comprises an antibacterial agent. In some embodiments, the pharmaceutical composition comprises m-cresol or methylparaben. In some embodiments, the pharmaceutical composition comprises 0.2% m-cresol and / or 0.9% methylparaben.

[0032] Uses of antibodies and pharmaceutical compositions In some embodiments, methods are provided for treating a companion animal species having an IL31-induced condition, comprising administering to the companion animal species a therapeutically effective amount of an anti-IL31 antibody described herein or a pharmaceutical composition comprising an antibody described herein. In some embodiments, the companion animal species is a dog, cat, or horse. In some embodiments, the IL31-induced condition is a pruritic or allergic condition. In some embodiments, the IL31-induced condition is selected from atopic dermatitis, pruritus, asthma, psoriasis, scleroderma, and eczema.

[0033] In some embodiments, the anti-IL31 antibody or pharmaceutical composition is administered parenterally, hi some embodiments, the anti-IL31 antibody or pharmaceutical composition is administered intramuscularly, intraperitoneally, intracerebrospinal, subcutaneously, intra-arterially, intrasynovially, intrathecally, or by inhalation.

[0034] In some embodiments, the methods comprise administering a Jak inhibitor, a PI3K inhibitor, an AKT inhibitor, or a MAPK inhibitor in combination with the anti-IL31 antibody or pharmaceutical composition. In some embodiments, the methods comprise administering one or more antibodies selected from an anti-IL17 antibody, an anti-TNFα antibody, an anti-CD20 antibody, an anti-CD19 antibody, an anti-CD25 antibody, an anti-IL4 antibody, an anti-IL13 antibody, an anti-IL23 antibody, an anti-IgE antibody, an anti-CD11α antibody, an anti-IL6R antibody, an anti-α4-integrin antibody, an anti-IL12 antibody, an anti-IL1β antibody, and an anti-BlyS antibody in combination with the anti-IL31 antibody or pharmaceutical composition.

[0035] In some embodiments, methods are provided for reducing IL31 signaling function in a cell, comprising exposing the cell to an anti-IL31 antibody or pharmaceutical composition described herein under conditions that allow binding of the antibody to extracellular IL31, thereby reducing binding to the IL31 receptor and / or reducing IL31 signaling function by the cell. In some embodiments, the cell is exposed to the antibody or pharmaceutical composition ex vivo. In some embodiments, the cell is exposed to the antibody or pharmaceutical composition in vivo. In some embodiments, the cell is a canine cell, a feline cell, or an equine cell.

[0036] In some embodiments, methods are provided for detecting IL31 in a sample from a companion animal species, comprising contacting the sample with an anti-IL31 antibody or pharmaceutical composition described herein under conditions that allow binding of the antibody to IL31, and detecting whether a complex is formed between the antibody and IL31 in the sample. In some embodiments, the sample is a biological sample obtained from a dog, cat, or horse. [Brief explanation of the drawings]

[0037] [Figure 1A] Alignment of the variable light sequences of the M14, M18, M19, and M87 mouse monoclonal antibody clones. [Figure 1B] Alignment of the variable heavy sequences of the M14, M18, M19, and M87 mouse monoclonal antibody clones. [Figure 2A-B] 10 is a graph of canine IL31 binding analysis using varying concentrations of chimeric M14 antibody. [Figure 3A-B] Fig. 10 depicts graphs showing the results of canine IL31 binding analysis using varying concentrations of caninized M14 antibody. [Figure 4] 1 is an immunoblot showing inhibition of canine IL31 signaling by varying concentrations of caninized M14 antibody. [Figure 5A-B] Immunoblots of GST-canine-IL31 deletion probed with M14 antibody and anti-GST antibody, respectively. [Figure 6A-B] Immunoblots of GST-canine-IL31 deletion probed with M14 antibody and anti-GST antibody, respectively. [Figure 7A-B] Immunoblots of feline and equine IL31 proteins fused to human Fc probed with M14 and anti-FC antibodies, respectively. [Figure 8] FIG. 1 shows immunoblot analysis of fine epitope mapping and alanine scanning of mature canine IL31 epitopes using anti-canine IL31 antibody (upper panel) and anti-GST antibody (lower panel). [Figure 9] FIG. 1 shows immunoblot analysis of fine epitope mapping and alanine scanning of mature canine IL31 epitopes using anti-canine IL31 antibody (upper panel) and anti-GST antibody (lower panel). [Figure 10] FIG. 1 shows immunoblot analysis of fine epitope mapping and alanine scanning of mature canine IL31 epitopes using anti-canine IL31 antibody (upper panel) and anti-GST antibody (lower panel). [Figure 11]FIG. 1 shows immunoblot analysis of fine epitope mapping and alanine scanning of mature canine IL31 epitopes using anti-canine IL31 antibody (upper panel) and anti-GST antibody (lower panel). [Figure 12] FIG. 1 shows immunoblot analysis of fine epitope mapping and alanine scanning of mature canine IL31 epitopes using anti-canine IL31 antibody (upper panel) and anti-GST antibody (lower panel). [Figure 13] Immunoblot cross-reactivity of anti-canine IL31 antibody M14 to walrus IL31. DETAILED DESCRIPTION OF THE INVENTION

[0038] Specific sequence description Table 1 provides a list of the specific sequences referenced herein. TIFF2025138636000002.tif249170TIFF2025138636000003.tif253170TIFF2025138636000004.t if254170TIFF2025138636000005.tif254170TIFF2025138636000006.tif254170TIFF20251386360 00007.tif254170TIFF2025138636000008.tif254170TIFF2025138636000009.tif254170TIFF202 5138636000010.tif254170TIFF2025138636000011.tif254170TIFF2025138636000012.tif242170

[0039] Description of Specific Embodiments Antibodies that bind to canine IL31, feline IL31, or equine IL31 are provided. Antibody heavy chains and antibody light chains capable of forming antibodies that bind to IL31 are also provided. Additionally, antibodies, heavy chains, and light chains comprising one or more specific complementarity-determining regions (CDRs) are provided. Polynucleotides encoding antibodies against canine IL31 are provided. Methods for producing or purifying antibodies against canine IL31 are also provided. Methods of treatment using antibodies against canine IL31 are provided. Such methods include, but are not limited to, methods for treating IL31-induced conditions in companion animal species. Methods for detecting IL31 in a sample from a companion animal species are provided.

[0040] For the convenience of the reader, the following definitions of terms used herein are provided.

[0041] As used herein, numerical terms such as Kd are calculated based on scientific measurements and, therefore, are subject to appropriate measurement errors. In some instances, the numerical terms may include values ​​that are rounded to the nearest significant figure.

[0042] As used herein, "a" or "an" means "at least one" or "one or more," unless otherwise specified. As used herein, the term "or" means "and / or," unless otherwise specified. In the context of multiple dependent claims, the use of "or" when referring back to other claims refers only to those claims in the alternative.

[0043] Anti-IL31 antibody Novel antibodies against IL31 are provided, for example, antibodies that bind to canine IL31, feline IL31, and / or equine IL31. The anti-IL31 antibodies provided herein include, but are not limited to, monoclonal antibodies, murine antibodies, chimeric antibodies, caninized antibodies, felineized antibodies, and equine antibodies. In some embodiments, the anti-IL31 antibody is an isolated murine monoclonal antibody, such as M14, M18, M19, and M87.

[0044] Monoclonal antibodies M14, M18, M19, and M87 were isolated as follows. Briefly, mice were immunized with canine IL31, and murine monoclonal antibody clones were obtained using standard hybridoma techniques. Enzyme-linked immunosorbent assay (ELISA) was used to screen hybridoma clones producing IL31-binding antibodies. Based on the binding affinity and cell-based functional assays described herein, hybridoma clones producing monoclonal antibodies M14, M18, M19, and M87 were selected for further study. The variable heavy chain (VH) and variable light chain (VL) of each of the four clones were sequenced and analyzed by sequence alignment (Figure 1).

[0045] The amino acid sequence of monoclonal antibody M14 is also provided herein. For example, for monoclonal antibody M14, the variable heavy chain CDRs (SEQ ID NOS: 1-3; SEQ ID NOS: 89 is an alternative definition of CDR-H2), variable light chain CDRs (SEQ ID NOS: 8-10), variable region heavy chain framework sequences (SEQ ID NOS: 4-7), and variable region light chain framework sequences (SEQ ID NOS: 11-14) are provided. The amino acid sequences of the variable light chain, light chain, variable heavy chain, and variable and hinge heavy chains of monoclonal antibody M14 are provided (SEQ ID NOS: 24, 36, 25, and 40, respectively).

[0046] Additionally, the amino acid sequences of the CDRs, framework sequences, variable light chains, and variable heavy chains of monoclonal antibodies M18, M19, and M87 are provided (see, e.g., Figure 1). For monoclonal antibody M19, the variable heavy chain CDRs (SEQ ID NOS: 1-3; SEQ ID NOS: 89 is an alternative definition of CDR-H2), variable light chain CDRs (SEQ ID NOS: 8-10), variable light chain (SEQ ID NOS: 65), light chain (SEQ ID NOS: 38), variable and hinge heavy chains (SEQ ID NOS: 42), and variable heavy chain (SEQ ID NOS: 68) are provided. For monoclonal antibody M18, the variable heavy chain CDRs (SEQ ID NOS: 1, 62, and 3; SEQ ID NOS: 87 is an alternative definition of CDR-H2), variable light chain CDRs (SEQ ID NOS: 63, 9, and 10), variable light chain (SEQ ID NOS: 64), light chain (SEQ ID NOS: 37), variable and hinge heavy chain (SEQ ID NOS: 41), and variable heavy chain (SEQ ID NOS: 67) are provided. The variable light chain (SEQ ID NO: 66), light chain (SEQ ID NO: 39), variable and hinge heavy chain (SEQ ID NO: 43), and variable heavy chain (SEQ ID NO: 69) for monoclonal antibody M87 are provided.

[0047] Also provided herein are chimeric, caninized, feline, and equine antibodies derived from monoclonal antibodies M14, M18, M19, and M87. In some embodiments, the amino acid sequence of caninized monoclonal antibody M14 is provided, such as SEQ ID NOs: 15-21 and 70-78. In some embodiments, the amino acid sequence of feline antibodies derived from monoclonal antibody M14 is provided, such as SEQ ID NOs: 32-35 and 79-86. In some embodiments, the amino acid sequence of chimeric antibodies derived from monoclonal antibody M14 is provided, such as SEQ ID NOs: 26, 27, 30, and 31.

[0048] The term "antibody" is used herein in the broadest sense and encompasses a variety of antibody structures, including, but not limited to, monoclonal antibodies, polyclonal antibodies, multispecific antibodies (e.g., bispecific (such as bispecific T cell engagers) and trispecific antibodies), and antibody fragments (such as Fab, F(ab'), ScFv, minibodies, diabodies, triabodies, and tetrabodies), so long as they exhibit the desired antigen-binding activity. Canine, feline, and equine species have different diversity (classes) of antibodies shared by many mammals.

[0049] The term "antibody" includes, but is not limited to, fragments capable of binding antigen, such as Fv, single-chain Fv (scFv), Fab, Fab', dis-scFv, sdAb (single-domain antibodies), and (Fab')2 (including chemically linked F(ab')2). Papain digestion of antibodies produces two identical antigen-binding fragments, called "Fab" fragments, each with a single antigen-binding site, and a residual "Fc" fragment, the name of which reflects its ability to readily crystallize. Pepsin treatment yields an F(ab')2 fragment with two antigen-binding sites and still capable of cross-linking antigen. The term "antibody" also includes, but is not limited to, chimeric antibodies, humanized antibodies, and antibodies from various species, such as murine, human, cynomolgus, canine, feline, equine, and others. Furthermore, for all antibody constructs provided herein, variants with sequences from other organisms are contemplated. Thus, given the disclosure of a murine version of an antibody, one skilled in the art would understand how to convert an antibody based on the murine sequence to a feline, canine, equine, or other sequence. Antibody fragments also include single-chain scFvs, tandem di-scFvs, diabodies, tandem tri-sdcFvs, minibodies, or any other orientation. Antibody fragments also include nanobodies (sdAbs, antibodies with a single monomeric domain without a light chain, such as a pair of heavy chain variable domains). Antibody fragments, in some embodiments, can also be referred to as being species specific (e.g., murine scFv or canine scFv). This refers to the sequence of at least a portion of the non-CDR regions, rather than the source of the construct. In some embodiments, the antibody comprises a label or is conjugated to a second moiety.

[0050] The terms "label" and "detectable label" refer to a moiety attached to an antibody or its analyte such that the reaction (e.g., binding) between members of a specific binding pair is detectable. A labeled member of a specific binding pair is said to be "detectably labeled." Thus, the term "labeled binding protein" refers to a protein incorporating a label that provides identification of the binding protein. In some embodiments, the label is a detectable marker capable of producing a signal detectable by visual or mechanical means, such as the incorporation of a radiolabeled amino acid or the attachment of a biotinylated moiety to the polypeptide that is detectable by marked avidin (e.g., streptavidin containing a fluorescent marker or enzymatic activity detectable by optical or colorimetric methods). Examples of labels for polypeptides include, but are not limited to, the following: radioisotopes or radionuclides (e.g., 3 H, 14 C. 35 S, 90 Y, 99 Tc, 111 In, 125 I, 131 I, 177 Lu, 166 Ho, or 153 Sm), chromogens, fluorescent labels (e.g., FITC, rhodamine, lanthanide phosphors), enzyme labels (e.g., horseradish peroxidase, luciferase, alkaline phosphatase), chemiluminescent markers, biotinyl groups, predetermined polypeptide epitopes recognized by secondary reporters (e.g., leucine zipper pair sequences, binding sites for secondary antibodies, metal binding domains, epitope tags), and magnetic agents such as gadolinium chelates. Representative examples of labels commonly employed for immunoassays include light-producing moieties, e.g., acridinium compounds, and fluorescence-producing moieties, e.g., fluorescein. In this regard, the moiety itself need not be detectably labeled, but may become detectable upon reaction with yet another moiety.

[0051] The term "monoclonal antibody" refers to an antibody from a substantially homogeneous population of antibodies. That is, the individual antibodies comprising the population are identical except for possible minor naturally occurring mutations. Monoclonal antibodies are highly specific, being directed against a single antigenic site. Furthermore, in contrast to polyclonal antibody preparations which typically include different antibodies directed against different determinants (epitopes), each monoclonal antibody is directed against a single determinant on the antigen. Thus, a sample of monoclonal antibodies is capable of binding to the same epitope on the antigen. The modifier "monoclonal" indicates the character of the antibody as being obtained from a substantially homogeneous population of antibodies and is not to be construed as requiring production of the antibody by any particular method. For example, monoclonal antibodies can be made by the hybridoma method first described by Kohler and Milstein, 1975, Nature 256:495, or can be made by recombinant DNA methods such as those described in U.S. Pat. No. 4,816,567. The monoclonal antibodies may also be isolated from phage libraries generated using the techniques described in McCafferty et al., 1990, Nature 348:552-554, for example.

[0052] In some embodiments, the monoclonal antibody is an isolated murine antibody selected from clones M14, M18, M19, and M87.

[0053] An "amino acid sequence" refers to the sequence of amino acid residues in a peptide or protein. The terms "polypeptide" and "protein" are used interchangeably and refer to a polymer of amino acid residues, without any minimum length restriction. Such polymers of amino acid residues may contain natural or unnatural amino acid residues, including, but not limited to, peptides, oligopeptides, dimers, trimers, and multimers of amino acid residues. This definition encompasses both full-length proteins and fragments thereof. The term also includes post-expression modifications of the polypeptide, such as glycosylation, sialylation, acetylation, phosphorylation, and the like. Furthermore, for purposes of this disclosure, "polypeptide" refers to a protein containing modifications to the native sequence, such as deletions, additions, and substitutions (generally conservative in nature), so long as the protein maintains the desired activity. These modifications may be deliberate, such as site-directed mutagenesis, or accidental, such as mutations in the host producing the protein or errors due to PCR amplification.

[0054] As used herein, "IL31" refers to any native IL31 resulting from intracellular expression and processing of IL31. Unless otherwise indicated, the term includes IL31 from any vertebrate source, including mammals such as primates (e.g., humans and cynomolgus monkeys) and rodents (e.g., mice and rats), and companion animals (e.g., dogs, cats, and horses). The term also includes naturally occurring variants of IL31, such as splice variants or allelic variants.

[0055] In some embodiments, canine IL31 comprises the amino acid sequence of SEQ ID NO:22 or SEQ ID NO:44. In some embodiments, feline IL31 comprises the amino acid sequence of SEQ ID NO:28. In some embodiments, equine IL31 comprises the amino acid sequence of SEQ ID NO:29. In some embodiments, human IL31 comprises the amino acid sequence of SEQ ID NO:46. In some embodiments, walrus IL31 comprises the amino acid sequence of SEQ ID NO:47. In some embodiments, mouse IL31 comprises the amino acid sequence of SEQ ID NO:61. In other embodiments, IL31 comprises the amino acid sequence of SEQ ID NO:49, SEQ ID NO:51, SEQ ID NO:52, SEQ ID NO:53, SEQ ID NO:54, SEQ ID NO:55, SEQ ID NO:56, SEQ ID NO:57, SEQ ID NO:58, SEQ ID NO:59, or SEQ ID NO:60.

[0056] The term "IL31-binding domain" of an antibody means the binding domain formed by the light and heavy chains of an anti-IL31 antibody that binds to IL31.

[0057] In some embodiments, the IL31-binding domain binds to canine IL31 with greater affinity than it binds to human IL31. In some embodiments, the IL31-binding domain binds to IL31 from other companion animals, such as feline IL31 or equine IL31. In some embodiments, the IL31-binding domain does not bind to human IL31.

[0058] As used herein, the term "epitope" refers to a site on a target molecule (e.g., an antigen, such as a protein, nucleic acid, carbohydrate, or lipid) to which an antigen-binding molecule (e.g., an antibody, antibody fragment, or scaffold protein containing an antibody-binding region) binds. Epitopes often comprise chemically active surface groupings of molecules, such as amino acids, polypeptides, or sugar side chains, and possess specific three-dimensional structural characteristics as well as specific charge characteristics. Epitopes can be formed from both contiguous or juxtaposed non-contiguous residues (e.g., amino acids, nucleotides, sugars, and lipid moieties) of a target molecule. Epitopes formed from contiguous residues (e.g., amino acids, nucleotides, sugars, and lipid moieties) are typically retained upon exposure to denaturing solvents, whereas epitopes formed by tertiary folding are typically lost upon treatment with denaturing solvents. Epitopes can include, but are not limited to, at least 3 residues, at least 5 residues, or 8-10 residues (e.g., amino acids or nucleotides). In some examples, the length of an epitope is less than 20 residues (e.g., amino acids or nucleotides), less than 15 residues, or less than 12 residues. If two antibodies exhibit competitive binding to an antigen, they may bind to the same epitope within the antigen. In some embodiments, an epitope can be identified by a certain minimum distance to the CDR residues of an antigen-binding molecule. In some embodiments, an epitope is identified by the above distance and further limited to those residues involved in binding (e.g., hydrogen bonding) between antibody and antigen residues. Epitopes can also be identified by a variety of scans; for example, scanning for alanine or arginine can reveal one or more residues with which an antigen-binding molecule can interact. Unless explicitly noted, a set of residues as an epitope does not exclude other residues from being part of the epitope for a particular antibody. Rather, the existence of such a set represents a minimum series (or species set) of epitopes. Thus, in some embodiments, the set of residues identified as the epitope represents the minimal epitope associated with the antigen, rather than an exclusive list of the epitope's residues on the antigen.

[0059] In some embodiments, the epitope comprises the amino acid sequence PSDX1X2KI (SEQ ID NO:45), where X is any amino acid residue. In some embodiments, X1 is a hydrophobic amino acid. In some embodiments, X1 is selected from A, V, I, and L. In some embodiments, X1 is selected from V and I. In some embodiments, X2 is a hydrophilic amino acid. In some embodiments, X2 is selected from A, R, K, Q, and N. In some embodiments, X2 is selected from R and Q. In some embodiments, X1 is V and X2 is R. In some embodiments, X1 is I and X2 is Q. In some embodiments, the epitope comprises the amino acid sequence of SEQ ID NO:88. In some embodiments, the epitope comprises the amino acid sequence of SEQ ID NO:23. In some embodiments, the epitope is within amino acids 34-50 of SEQ ID NO:22. In some embodiments, the epitope comprises amino acids 34-50 of SEQ ID NO:22.

[0060] The term "CDR" refers to a complementarity determining region defined by at least one manner of identification to one of ordinary skill in the art. In some embodiments, CDRs can be defined according to any of the Chothia numbering scheme, the Kabat numbering scheme, a combination of Kabat and Chothia, the AbM definition, the contact definition, or a combination of the Kabat, Chothia, AbM, or contact definitions. The various CDRs in an antibody can be represented by appropriate numbers and chain types, including, but not limited to, CDR-H1, CDR-H2, CDR-H3, CDR-L1, CDR-L2, and CDR-L3. The term "CDR" is used herein to encompass "hypervariable regions" or HVRs, which include the hypervariable loops.

[0061] In some embodiments, the anti-IL31 antibody comprises a heavy chain comprising (a) a CDR-H1 comprising the amino acid sequence of SEQ ID NO: 1, (b) a CDR-H2 comprising the amino acid sequence of SEQ ID NO: 2, SEQ ID NO: 62, SEQ ID NO: 89, or SEQ ID NO: 87, or (c) a CDR-H3 comprising the amino acid sequence of SEQ ID NO: 3. In some embodiments, the anti-IL31 antibody comprises a light chain comprising (a) a CDR-L1 comprising the amino acid sequence of SEQ ID NO: 8 or SEQ ID NO: 63, (b) a CDR-L2 comprising the amino acid sequence of SEQ ID NO: 9, or (c) a CDR-L3 comprising the amino acid sequence of SEQ ID NO: 10.

[0062] In some embodiments, the anti-IL31 antibody comprises a heavy chain comprising (a) a CDR-H1 comprising the amino acid sequence of SEQ ID NO: 1, (b) a CDR-H2 comprising the amino acid sequence of SEQ ID NO: 2 or 89, and (c) a CDR-H3 comprising the amino acid sequence of SEQ ID NO: 3, and a light chain comprising (a) a CDR-L1 comprising the amino acid sequence of SEQ ID NO: 8 or 63, (b) a CDR-L2 comprising the amino acid sequence of SEQ ID NO: 9, and (c) a CDR-L3 comprising the amino acid sequence of SEQ ID NO: 10.

[0063] In some embodiments, the anti-IL31 antibody comprises a heavy chain comprising (a) a CDR-H1 comprising the amino acid sequence of SEQ ID NO: 1, (b) a CDR-H2 comprising the amino acid sequence of SEQ ID NO: 62 or 87, and (c) a CDR-H3 comprising the amino acid sequence of SEQ ID NO: 3, and a light chain comprising (a) a CDR-L1 comprising the amino acid sequence of SEQ ID NO: 8 or 63, (b) a CDR-L2 comprising the amino acid sequence of SEQ ID NO: 9, and (c) a CDR-L3 comprising the amino acid sequence of SEQ ID NO: 10.

[0064] The term "variable region," as used herein, refers to a region comprising at least three CDRs. In some embodiments, a variable region comprises three CDRs and at least one framework region ("FR"). The terms "heavy chain variable region" or "variable heavy chain" are used interchangeably to refer to a region comprising at least three heavy chain CDRs. The terms "light chain variable region" or "variable light chain" are used interchangeably to refer to a region comprising at least three light chain CDRs. In some embodiments, a variable heavy chain or variable light chain comprises at least one framework region. In some embodiments, an antibody comprises at least one heavy chain framework region selected from HC-FR1, HC-FR2, HC-FR3, and HC-FR4. In some embodiments, an antibody comprises at least one light chain framework region selected from LC-FR1, LC-FR2, LC-FR3, and LC-FR4. The framework region may be juxtaposed between the light chain CDRs or between the heavy chain CDRs. For example, the antibody may comprise a variable heavy chain having the following structure: (HC-FR1)-(CDR-H1)-(HC-FR2)-(CDR-H2)-(HC-FR3)-(CDR-H3)-(HC-FR4). The antibody may comprise a variable heavy chain having the following structure: (CDR-H1)-(HC-FR2)-(CDR-H2)-(HC-FR3)-(CDR-H3). The antibody may comprise a variable light chain having the following structure: (LC-FR1)-(CDR-L1)-(LC-FR2)-(CDR-L2)-(LC-FR3)-(CDR-L3)-(LC-FR4). The antibody may comprise a variable light chain having the following structure: (CDR-L1)-(LC-FR2)-(CDR-L2)-(LC-FR3)-(CDR-L3).

[0065] In some embodiments, the anti-IL31 antibody comprises one or more of: (a) the variable region heavy chain framework 1 (HC-FR1) sequence of SEQ ID NO: 4, (b) the HC-FR2 sequence of SEQ ID NO: 5, (c) the HC-FR3 sequence of SEQ ID NO: 6, (d) the HC-FR4 sequence of SEQ ID NO: 7, (e) the variable region light chain framework 1 (LC-FR1) sequence of SEQ ID NO: 11, (f) the LC-FR2 sequence of SEQ ID NO: 12, (g) the LC-FR3 sequence of SEQ ID NO: 13, or (h) the LC-FR4 sequence of SEQ ID NO: 14. In some embodiments, the anti-IL31 antibody comprises a variable light chain sequence of (a) SEQ ID NO: 16, (b) SEQ ID NO: 24, or (c) SEQ ID NO: 32. In some embodiments, the anti-IL31 antibody comprises a variable heavy chain sequence of (a) SEQ ID NO: 15, (b) SEQ ID NO: 25, or (c) SEQ ID NO: 33. In some embodiments, the anti-IL31 antibody comprises (a) the variable light chain sequence of SEQ ID NO: 16 and the variable heavy chain sequence of SEQ ID NO: 15, (b) the variable light chain sequence of SEQ ID NO: 24 and the variable heavy chain sequence of SEQ ID NO: 25, or (c) the variable light chain sequence of SEQ ID NO: 32 and the variable heavy chain sequence of SEQ ID NO: 33.

[0066] The term "constant region," as used herein, refers to a region comprising at least three constant domains. The terms "heavy chain constant region" or "constant heavy chain" are used interchangeably and refer to a region comprising at least three heavy chain constant domains, CH1, CH2, and CH3. Non-limiting exemplary heavy chain constant regions include gamma, delta, alpha, epsilon, and mu. Each heavy chain constant region corresponds to the antibody's isotype. For example, an antibody comprising a gamma constant region is an IgG antibody, a delta constant region is an IgD antibody, an alpha constant region is an IgA antibody, a mu constant region is an IgM antibody, and an epsilon constant region is an IgE antibody. A particular isotype can be further subdivided into subclasses. For example, IgG antibodies include, but are not limited to, IgG1 (containing a γ1 constant region), IgG2 (containing a γ2 constant region), IgG3 (containing a γ3 constant region), and IgG4 (containing a γ4 constant region) antibodies; IgA antibodies include, but are not limited to, IgA1 (containing an α1 constant region) and IgA2 (containing an α2 constant region) antibodies; and IgM antibodies include, but are not limited to, IgM1 and IgM2. The terms "light chain constant region" or "constant light chain" are used interchangeably and refer to the region containing the light chain constant domain CL. Non-limiting exemplary light chain constant regions include λ and κ. Deletions and changes within domains that do not alter function are encompassed within the term "constant region" unless otherwise specified. Dogs, cats, and horses have antibody classes such as IgG, IgA, IgD, IgE, and IgM. Canine IgG antibody classes include IgG-A, IgG-B, IgG-C, and IgG-D. Feline IgG antibody classes include IgG1a, IgG1b, and IgG2. Equine IgG antibody classes include IgG1, IgG2, IgG3, IgG4, IgG5, IgG6, and IgG7.

[0067] The term "chimeric antibody" or "chimera" refers to an antibody in which a portion of the heavy or light chain is derived from a particular source or species, while at least a portion of the remaining heavy or light chain is derived from a different source or species. In some embodiments, a chimeric antibody refers to an antibody that comprises at least one variable region from a first species (e.g., mouse, rat, cynomolgus monkey, etc.) and at least one constant region from a second species (e.g., human, dog, cat, horse, etc.). In some embodiments, a chimeric antibody comprises at least one murine variable region and at least one canine constant region. In some embodiments, a chimeric antibody comprises at least one murine variable region and at least one feline constant region. In some embodiments, all of the variable regions of a chimeric antibody are from a first species and all of the constant regions of a chimeric antibody are from a second species. In some embodiments, a chimeric antibody comprises a constant heavy chain region or a constant light chain region from a companion animal. In some embodiments, a chimeric antibody comprises murine variable heavy and light chains and constant heavy and light chains from a companion animal. For example, a chimeric antibody may comprise murine variable heavy and light chains and canine constant heavy and light chains; a chimeric antibody may comprise murine variable heavy and light chains and feline constant heavy and light chains; or a chimeric antibody may comprise murine variable heavy and light chains and equine constant heavy and light chains.

[0068] In some embodiments, the anti-IL31 antibody a. (i) the light chain amino acid sequence of SEQ ID NO: 26, (ii) the heavy chain amino acid sequence of SEQ ID NO: 27, or (iii) the light chain amino acid sequence of (i) and the heavy chain sequence of (ii), or b. Includes chimeric antibodies comprising (i) the light chain amino acid sequence of SEQ ID NO: 30, (ii) the heavy chain amino acid sequence of SEQ ID NO: 31, or (iii) the light chain amino acid sequence of (i) and the heavy chain sequence of (ii).

[0069] "Canine chimera" or "canine chimeric antibody" refers to a chimeric antibody having at least a portion of its heavy chain or light chain derived from a canine. "Cat chimera" or "cat chimeric antibody" refers to a chimeric antibody having at least a portion of its heavy chain or light chain derived from a feline. "Equine chimera" or "equine chimeric antibody" refers to a chimeric antibody having at least a portion of its heavy chain or light chain derived from a horse. In some embodiments, a canine chimeric antibody comprises murine variable heavy and light chains and canine constant heavy and light chains. In some embodiments, a cat chimeric antibody comprises murine variable heavy and light chains and feline constant heavy and light chains. In some embodiments, an equine chimeric antibody comprises murine variable heavy and light chains and equine constant heavy and light chains. In some embodiments, the antibody is a chimeric antibody comprising a murine variable heavy chain framework region or a murine variable light chain framework region.

[0070] "Canine antibodies," as used herein, include antibodies produced in canines, antibodies produced in non-canine animals that contain canine immunoglobulin genes or that contain canine immunoglobulin peptides, or antibodies selected using in vitro methods such as phage display, where the antibody repertoire is based on canine immunoglobulin sequences. The term "canine antibodies" refers to the genus of sequences that are canine sequences. Thus, the term does not refer to the process of creating the antibody, but rather to the genus of related sequences.

[0071] In some embodiments, the anti-IL31 antibody comprises a canine heavy chain constant region selected from an IgG-A, IgG-B, IgG-C, and IgG-D constant region. In some embodiments, the anti-IL31 antibody is a canine IgG-A, IgG-B, IgG-C, or IgG-D antibody. In some embodiments, the anti-IL31 antibody is (a) a canine IgG-A antibody comprising the heavy chain amino acid sequence of SEQ ID NO: 17, (b) a canine IgG-B antibody comprising the heavy chain amino acid sequence of SEQ ID NO: 18, (c) a canine IgG-C antibody comprising the heavy chain amino acid sequence of SEQ ID NO: 19, or (d) a canine IgG-D antibody comprising the heavy chain amino acid sequence of SEQ ID NO: 20.

[0072] "Feline antibodies," as used herein, encompass antibodies produced in cats, antibodies produced in non-feline animals that contain feline immunoglobulin genes or that contain feline immunoglobulin peptides, or antibodies selected using in vitro methods such as phage display, where the antibody repertoire is based on feline immunoglobulin sequences. The term "feline antibodies" refers to the genus of sequences that are feline sequences. Thus, the term does not refer to the process of creating the antibody, but rather to the genus of related sequences.

[0073] In some embodiments, the anti-IL31 antibody comprises a feline heavy chain constant region selected from an IgG1, IgG2a, and IgG2b constant region, hi some embodiments, the anti-IL31 antibody is a feline IgG1, IgG2a, or IgG2b antibody.

[0074] "Equine antibodies," as used herein, include antibodies produced in horses, antibodies produced in non-equine animals that contain equine immunoglobulin genes or that contain equine immunoglobulin peptides, or antibodies selected using in vitro methods such as phage display in which the antibody repertoire is based on equine immunoglobulin sequences. The term "equine antibodies" refers to the genus of sequences that are equine sequences. Thus, the term does not refer to the process of creating the antibody, but rather to the genus of related sequences.

[0075] In some embodiments, the anti-IL31 antibody comprises an equine heavy chain constant region selected from an IgG1, IgG2, IgG3, IgG4, IgG5, IgG6, and IgG7 constant region, hi some embodiments, the anti-IL31 antibody is an equine IgG1, IgG2, IgG3, IgG4, IgG5, IgG6, and IgG7 antibody.

[0076] A "caninized antibody" refers to an antibody in which at least one amino acid in a portion of a non-canine variable region has been replaced with the corresponding amino acid from a canine variable region. In some embodiments, a caninized antibody comprises at least one canine constant region (e.g., a gamma constant region, an alpha constant region, a delta constant region, an epsilon constant region, a mu constant region, or other) or fragment thereof. In some embodiments, a caninized antibody is a Fab, scFv, (Fab')2, or other antibody fragment. The term "caninized" also refers to forms of non-canine (e.g., murine) antibodies that are chimeric immunoglobulins, immunoglobulin chains, or fragments thereof (such as Fv, Fab, Fab', F(ab')2, or other antigen-binding sequences of antibodies) that comprise minimal non-canine immunoglobulin sequence. A caninized antibody may comprise a canine immunoglobulin (recipient antibody) in which residues from the recipient's CDRs have been replaced by residues from the CDRs of a non-canine species (donor antibody), such as mouse, rat, or rabbit, having the desired specificity, affinity, and capacity. In some instances, Fv framework region (FR) residues of the canine immunoglobulin are replaced by corresponding non-canine residues. Furthermore, caninized antibodies may comprise residues that are found neither in the recipient antibody nor in the imported CDR or framework sequences, but are included to further refine and optimize antibody performance.

[0077] In some embodiments, at least one amino acid residue in a portion of the murine variable heavy chain or the murine variable light chain is replaced with the corresponding amino acid from a canine variable region. In some embodiments, the modified chain is fused to a canine constant heavy chain or a canine constant light chain. In some embodiments, the anti-IL31 antibody is a caninized antibody comprising (a) the heavy chain sequence of SEQ ID NO: 15, (b) the heavy chain sequence of SEQ ID NO: 17, (c) the heavy chain sequence of SEQ ID NO: 18, (d) the heavy chain sequence of SEQ ID NO: 19, (e) the heavy chain sequence of SEQ ID NO: 20, (f) the light chain sequence of SEQ ID NO: 16, or (g) the light chain sequence of SEQ ID NO: 21.

[0078] A "felineized antibody" refers to an antibody in which at least one amino acid in a portion of a non-feline variable region has been replaced with the corresponding amino acid from a feline variable region. In some embodiments, a felineized antibody comprises at least one feline constant region (e.g., a gamma constant region, an alpha constant region, a delta constant region, an epsilon constant region, a mu constant region, or other) or fragment thereof. In some embodiments, a felineized antibody is a Fab, scFv, (Fab')2, or other antibody fragment. The term "felineized" also refers to forms of non-feline (e.g., murine) antibodies that are chimeric immunoglobulins, immunoglobulin chains, or fragments thereof (e.g., Fv, Fab, Fab', F(ab')2, or other antigen-binding sequences of antibodies) that comprise minimal non-feline immunoglobulin sequence. A felineized antibody may comprise a feline immunoglobulin (recipient antibody) in which residues from the recipient's CDRs have been replaced by residues from the CDRs of a non-feline species (donor antibody), such as mouse, rat, or rabbit, that has the desired specificity, affinity, and capacity. In some instances, Fv framework region (FR) residues of the feline immunoglobulin are replaced by corresponding non-feline residues. Furthermore, felineized antibodies may comprise residues that are found neither in the recipient antibody nor in the imported CDR or framework sequences, but are included to further refine and optimize antibody performance.

[0079] In some embodiments, at least one amino acid residue in a portion of the murine variable heavy chain or the murine variable light chain is replaced with the corresponding amino acid from a feline variable region. In some embodiments, the modified chain is fused to a feline constant heavy chain or a canine constant light chain. In some embodiments, the anti-IL31 antibody is a felinized antibody comprising (a) the light chain sequence of SEQ ID NO: 32, (b) the light chain sequence of SEQ ID NO: 34, (c) the heavy chain sequence of SEQ ID NO: 33, or (d) the heavy chain sequence of SEQ ID NO: 35.

[0080] An "equinized antibody" refers to an antibody in which at least one amino acid in a portion of a non-equine variable region has been replaced with the corresponding amino acid from an equine variable region. In some embodiments, an equine antibody comprises at least one equine constant region (e.g., a gamma constant region, an alpha constant region, a delta constant region, an epsilon constant region, a mu constant region, or other) or fragment thereof. In some embodiments, an equine antibody is a Fab, scFv, (Fab')2, or other antibody fragment. The term "equinized" also refers to forms of non-equine (e.g., murine) antibodies that are chimeric immunoglobulins, immunoglobulin chains, or fragments thereof (such as Fv, Fab, Fab', F(ab')2, or other antigen-binding sequences of antibodies) that comprise minimal non-equine immunoglobulin sequence. An equine antibody may comprise an equine immunoglobulin (recipient antibody) in which residues from the recipient's CDRs have been replaced by residues from the CDRs of a non-equine species (donor antibody), such as mouse, rat, or rabbit, having the desired specificity, affinity, and capacity. In some instances, Fv framework region (FR) residues of the equine immunoglobulin are replaced by corresponding non-equine residues. Furthermore, equine-modified antibodies may comprise residues that are found neither in the recipient antibody nor in the imported CDR or framework sequences, but are included to further refine and optimize antibody performance.

[0081] In some embodiments, at least one amino acid residue in a portion of a murine variable heavy chain or a murine variable light chain is replaced with the corresponding amino acid from an equine variable region, hi some embodiments, the modified chain is fused to an equine constant heavy chain or a canine constant light chain.

[0082] The term "IgX Fc" means that the Fc region is derived from a particular antibody isotype (e.g., IgG, IgA, IgD, IgE, IgM, etc.), where "X" refers to the antibody isotype. Thus, "IgG Fc" refers to the Fc region of the gamma chain, "IgA Fc" refers to the Fc region of the alpha chain, "IgD Fc" refers to the Fc region of the delta chain, "IgE Fc" refers to the Fc region of the epsilon chain, "IgM Fc" refers to the Fc region of the mu chain, etc. In some embodiments, an IgG Fc region comprises a CH1, hinge, CH2, CH3, and CL1. "IgX-N-Fc" means that the Fc region is derived from a specific subclass of antibody isotype (e.g., canine IgG subclasses A, B, C, or D; feline IgG subclasses 1, 2a, or 2b; or equine IgG subclasses IgG1, IgG2, IgG3, IgG4, IgG5, IgG6, or IgG7, etc.), where "N" refers to subclass. In some embodiments, the IgX Fc or IgX-N-Fc region is derived from a companion animal such as a dog, cat, or horse. In some embodiments, the IgG Fc region is isolated from a canine gamma heavy chain, such as IgG-A, IgG-B, IgG-C, or IgG-D. In some examples, the IgG Fc region is isolated from a feline gamma heavy chain, such as IgG1, IgG2a, or IgG2b. Antibodies comprising an IgG-A, IgG-B, IgG-C, or IgG-D Fc region may provide high expression levels in recombinant production systems.

[0083] The term "affinity" refers to the strength of the sum of all non-covalent interactions between a single binding site of a molecule (e.g., an antibody) and its binding partner (e.g., an antigen). The affinity of a molecule X for its partner Y is generally measured by the dissociation constant (K D Affinity can be measured by common methods known in the art, such as immunoblot, ELISA KD, KinEx A, biolayer interferometry (BLI), or surface plasmon resonance device.

[0084] The term “K D "," "K d", "Kd," or "Kd value" are used interchangeably and refer to the equilibrium dissociation constant of an antibody-antigen interaction. In some embodiments, the K d is measured using biolayer interferometry with a biosensor such as the Octet® system (Pall ForteBio LLC, Fremont, CA) according to the supplier's instructions. Briefly, biotinylated antigen is bound to the sensor chip, and antibody association is monitored for 90 seconds and dissociation for 600 seconds. The buffer for the dilution and binding steps is 20 mM phosphate, 150 mM NaCl, pH 7.2. A buffer-only blank curve is subtracted to correct for any drift. Data are analyzed to determine the association rate constant (k on ), dissociation rate constant (k off ), and K d Fitting to a 2:1 binding model using ForteBio data analysis software to determine the equilibrium dissociation constant (K d ) is k off / k on The term "k on " refers to the rate constant for the association of an antibody to an antigen, and the term "k off " means the rate constant for dissociation of an antibody from the antibody / antigen complex.

[0085] The term "binding" to an antigen or epitope is a term well understood in the art, and methods for determining such binding are also well known in the art. A molecule is said to exhibit "binding" if it reacts with, associates with, or has affinity for a particular cell or substance, and that reaction, association, or affinity is detectable by one or more methods known in the art, such as immunoblot, ELISA KD, KinEx A, biolayer interferometry (BLI), surface plasmon resonance devices, and the like.

[0086] "Surface plasmon resonance" refers to an optical phenomenon that allows for the analysis of real-time bispecific interactions by detecting changes in protein concentration within a biosensor matrix using, for example, the BIAcore™ system (BIACore International AB, a GE Healthcare Company, Uppsala, Sweden and Piscataway, NJ). For further explanation, see Jonsson et al. (1993) Ann. Biol. Clin. 51:19-26.

[0087] "Bio-layer interferometry" refers to an optical analysis technique that analyzes the interference pattern of light reflected from a layer of immobilized proteins and an internal reference layer on a biosensor chip. Changes in the number of molecules bound to the biosensor chip change the interference pattern, which can be measured in real time. A non-limiting exemplary device for bio-layer interferometry is the Octet® system (Pall ForteBio LLC). See, e.g., Abdiche et al., 2008, Anal. Biochem. 377:209-277.

[0088] In some embodiments, the anti-IL31 antibody has a concentration of 5×10 -6 Less than M, 1 x 10 -6 Less than M, 5 x 10 -7 Less than M, 1 x 10 -7 Less than M, 5 x 10 -8 Less than M, 1 x 10 -8 Less than M, 5 x 10 -9 Less than M, 1 x 10 -9 Less than M, 5 x 10 -10 Less than M, 1 x 10 -10 Less than M, 5 x 10 -11 Less than M, 1 x 10 -11 Less than M, 5 x 10 -12 Less than M or 1 x 10 -12 In some embodiments, the anti-IL31 antibody binds to canine IL31, feline IL31, or equine IL31 with a dissociation constant (Kd) of less than 5×10 M as measured by biolayer interferometry. -6 M~1×10-6 M、5×10 -6 M~5×10 -7 M、5×10 -6 M~1×10 -7 M、5×10 -6 M~5×10 -8 M、5×10 -6 M~1×10 -8 M、5×10 -6 M~5×10 -9 M、5×10 -6 M~1×10 -9 M、5×10 -6 M~5×10 -10 M、5×10 -6 M~1×10 -10 M、5×10 -6 M~5×10 -11 M、5×10 -6 M~1×10 -11 M、5×10 -6 M~5×10 -12 M、5×10 -6 M~1×10 -12 M、1×10 -6 M~5×10 -7 M、1×10 -6 M~1×10 -7 M、1×10 -6 M~5×10 -8 M、1×10 -6 M~1×10 -8 M、1×10 -6 M~5×10 -9 M、1×10 -6 M~1×10 -9 M、1×10 -6 M~5×10 -10 M、1×10 -6 M~1×10 -10 M、1×10 -6 M~5×10 -11 M、1×10 -6 M~1×10 -11 M、1×10 -6 M~5×10 -12 M、1×10 -6 M~1×10 -12 M、5×10 -7 M~1×10 -7 M、5×10 -7 M~5×10 -8M、5×10 -7 M~1×10 -8 M、5×10 -7 M~5×10 -9 M、5×10 -7 M~1×10 -9 M、5×10 -7 M~5×10 -10 M、5×10 -7 M~1×10 -10 M、5×10 -7 M~5×10 -11 M、5×10 -7 M~1×10 -11 M、5×10 -7 M~5×10 -12 M、5×10 -7 M~1×10 -12 M、1×10 -7 M~5×10 -8 M、1×10 -7 M~1×10 -8 M、1×10 -7 M~5×10 -9 M、1×10 -7 M~1×10 -9 M、1×10 -7 M~5×10 -10 M、1×10 -7 M~1×10 -10 M、1×10 -7 M~5×10 -11 M、1×10 -7 M~1×10 -11 M、1×10 -7 M~5×10 -12 M、1×10 -7 M~1×10 -12 M、5×10 -8 M~1×10 -8 M、5×10 -8 M~5×10 -9 M、5×10 -8 M~1×10 -9 M、5×10 -8 M~5×10 -10 M、5×10 -8 M~1×10 -10 M、5×10 -8 M~5×10 -11 M、5×10 -8 M~1×10 -11 M、5×10-8 M~5×10 -12 M、5×10 -8 M~1×10 -12 M、1×10 -8 M~5×10 -9 M、1×10 -8 M~1×10 -9 M、1×10 -8 M~5×10 -10 M、1×10 -8 M~1×10 -10 M、1×10 -8 M~5×10 -11 M、1×10 -8 M~1×10 -11 M、1×10 -8 M~5×10 -12 M、1×10 -8 M~1×10 -12 M、5×10 -9 M~1×10 -9 M、5×10 -9 M~5×10 -10 M、5×10 -9 M~1×10 -10 M、5×10 -9 M~5×10 -11 M、5×10 -9 M~1×10 -11 M、5×10 -9 M~5×10 -12 M、5×10 -9 M~1×10 -12 M、1×10 -9 M~5×10 -10 M、1×10 -9 M~1×10 -10 M、1×10 -9 M~5×10 -11 M、1×10 -9 M~1×10 -11 M、1×10 -9 M~5×10 -12 M、1×10 -9 M~1×10 -12 M、5×10 -10 M~1×10 -10 M、5×10 -10 M~5×10 -11 M、1×10 -10 M~5×10 -11 M、1×10 -10M~1×10 -11 M, 1 x 10 -10 M~5×10 -12 M, 1 x 10 -10 M~1×10 -12 M, 5 x 10 -11 M~1×10 -12 M, 5 x 10 -11 M~5×10 -12 M, 5 x 10 -11 M~1×10 -12 M, 1 x 10 -11 M~5×10 -12 M, or 1 x 10 -11 M~1×10 -12 It binds to canine IL31, feline IL31, or equine IL31 with a Kd of M. In some embodiments, the anti-IL31 antibody binds to canine IL31, feline IL31, or equine IL31 as determined by immunoblot analysis.

[0089] In some embodiments, the anti-IL31 antibody does not bind to human IL31 as determined by immunoblot analysis and / or biolayer interferometry.

[0090] In some embodiments, anti-IL31 antibodies are provided that compete with the anti-IL31 antibodies described herein (e.g., M14, M18, M19, or M87) for binding to IL31. In some embodiments, antibodies can be made or used that compete for binding with any of the antibodies provided herein. In some embodiments, anti-IL31 antibodies are provided that compete with the monoclonal M14 antibody for binding to canine IL31 or feline IL31.

[0091] "Variant" means a biologically active polypeptide having at least about 50% amino acid sequence identity with a native sequence polypeptide, after aligning the sequences and introducing gaps, if necessary, to maximize the percent sequence identity, and not counting conservative substitutions as part of the sequence identity. Such variants include, for example, polypeptides having one or more amino acid residues added or deleted at the N- or C-terminus of the polypeptide.

[0092] In some embodiments, variants have at least about 50% amino acid sequence identity, at least about 60% amino acid sequence identity, at least about 65% amino acid sequence identity, at least about 70% amino acid sequence identity, at least about 75% amino acid sequence identity, at least about 80% amino acid sequence identity, at least about 85% amino acid sequence identity, at least about 90% amino acid sequence identity, or at least about 95% amino acid sequence identity to a native sequence polypeptide.

[0093] As used herein, "percent amino acid sequence identity" and "homology" with respect to peptide, polypeptide, or antibody sequences are defined as the percentage of amino acid residues in a candidate sequence that are identical with those in a particular peptide or polypeptide sequence, after aligning the sequences and introducing gaps, if necessary, to maximize the percent sequence identity, without considering conservative substitutions as part of the sequence identity. Alignment for purposes of determining percent amino acid sequence identity can be achieved by a variety of methods within the art, for example, using publicly available computer software such as BLAST, BLAST-2, ALIGN, or MEGALINE™ (DNASTAR) software. Those skilled in the art can determine appropriate parameters for measuring alignment, including any algorithms needed to achieve maximal alignment over the full length of the sequences being compared.

[0094] Amino acid substitutions can include, but are not limited to, replacing one amino acid in a polypeptide with another. Exemplary substitutions are shown in Table 2. Amino acid substitutions can be introduced into an antibody of interest and the products screened for a desired activity, such as retained / improved antigen binding, reduced immunogenicity, or improved ADCC or CDC. Table 2 TIFF2025138636000013.tif208170

[0095] Amino acids can be grouped according to common side chain properties. (1) Hydrophobic: Norleucine, Met, Ala, Val, Leu, Ile; (2) Neutral hydrophilic: Cys, Ser, Thr, Asn, Gln; (3) Acidic: Asp, Glu; (4) basic: His, Lys, Arg; (5) Residues that affect chain orientation: Gly, Pro; (6) Aromatic: Trp, Tyr, Phe.

[0096] Non-conservative substitutions will involve exchanging a member of one of these classes for another class.

[0097] In some embodiments, the anti-IL31 antibody comprises a heavy chain and a light chain: a. the heavy chain comprises a CDR-H1 sequence having at least 85% sequence identity, at least 90% sequence identity, at least 95% sequence identity, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO:1, a CDR-H2 sequence having at least 85% sequence identity, at least 90% sequence identity, at least 95% sequence identity, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO:2, SEQ ID NO:62, SEQ ID NO:89, or SEQ ID NO:87, and a CDR-H3 sequence having at least 85% sequence identity, at least 90% sequence identity, at least 95% sequence identity, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO:3; b. The light chain comprises a CDR-L1 sequence having at least 85% sequence identity, at least 90% sequence identity, at least 95% sequence identity, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO:8 or SEQ ID NO:63, a CDR-L2 sequence having at least 85% sequence identity, at least 90% sequence identity, at least 95% sequence identity, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO:9, and a CDR-L3 sequence having at least 85% sequence identity, at least 90% sequence identity, at least 95% sequence identity, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO:10.

[0098] In some embodiments, the anti-IL31 antibody comprises a heavy chain and a light chain: a. (i) a variable light chain sequence having at least 85%, at least 90%, at least 95%, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO: 24, (ii) a variable heavy chain sequence having at least 85%, at least 90%, at least 95%, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO: 25, or (iii) the variable light chain sequence of (i) and the variable heavy chain sequence of (ii), or b. (i) a variable light chain sequence having at least 85%, at least 90%, at least 95%, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO: 16; (ii) a variable heavy chain sequence having at least 85%, at least 90%, at least 95%, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO: 15; or (iii) the variable light chain sequence of (i) and the variable heavy chain sequence of (ii); or c. (i) a variable light chain sequence having at least 85%, at least 90%, at least 95%, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO: 32; (ii) a variable heavy chain sequence having at least 85%, at least 90%, at least 95%, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO: 33; or (iii) the variable light chain sequence of (i) and the variable heavy chain sequence of (ii); or d. (i) a variable light chain sequence having at least 85%, at least 90%, at least 95%, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO: 64; (ii) a variable heavy chain sequence having at least 85%, at least 90%, at least 95%, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO: 67; or (iii) the variable light chain sequence of (i) and the variable heavy chain sequence of (ii); or e. (i) a variable light chain sequence having at least 85%, at least 90%, at least 95%, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO: 65; (ii) a variable heavy chain sequence having at least 85%, at least 90%, at least 95%, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO: 68; or (iii) the variable light chain sequence of (i) and the variable heavy chain sequence of (ii); or f. (i) a variable light chain sequence having at least 85%, at least 90%, at least 95%, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO: 66; (ii) a variable heavy chain sequence having at least 85%, at least 90%, at least 95%, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO: 69; or (iii) the variable light chain sequence of (i) and the variable heavy chain sequence of (ii).

[0099] The term "vector" is used to describe a polynucleotide that can be engineered to contain a cloned polynucleotide or a polynucleotide that can be propagated in a host cell. A vector may contain one or more of the following elements: an origin of replication, one or more regulatory sequences (such as a promoter or enhancer) that control the expression of a polypeptide of interest, or one or more selectable marker genes (such as antibiotic resistance genes and genes that can be used in colorimetric assays, e.g., β-galactosidase). The term "expression vector" refers to a vector used to express a polypeptide of interest in a host cell.

[0100] "Host cell" refers to a cell that can be or has been the recipient of a vector or isolated polynucleotide. Host cells can be prokaryotic or eukaryotic. Exemplary eukaryotic cells include mammalian cells, such as primate or non-primate cells, fungal cells, such as yeast, plant cells, and insect cells. Non-limiting exemplary mammalian cells include, but are not limited to, NSO cells, PER.C6® cells (Crucell), 293 cells, and CHO cells, as well as their derivatives, such as 293-6E, DG44, CHO-S, and CHO-K cells. A host cell includes the progeny of a single host cell; the progeny may not necessarily be completely identical (in morphology or genomic DNA complement) to the original parent cell due to natural, accidental, or deliberate mutation. A host cell includes cells transfected in vivo with a polynucleotide encoding the amino acid sequences provided herein.

[0101] The term "isolated," as used herein, refers to a molecule that has been separated from at least some of the components with which it is typically found or produced in nature. For example, a polypeptide is referred to as "isolated" if it has been separated from at least some of the components of the cell that produced it. If the polypeptide is secreted from the cell after expression, physically separating the supernatant containing the polypeptide from the cell that produced it is considered to "isolate" the polypeptide. Similarly, a polynucleotide is referred to as "isolated" if it is not part of a larger polynucleotide (e.g., genomic DNA or mitochondrial DNA, in the case of a DNA polynucleotide) with which it is typically found in nature, or if it has been separated from at least some of the components of the cell that produced it, in the case of an RNA polynucleotide, for example. Thus, a DNA polynucleotide contained in a vector within a host cell may be referred to as "isolated." In some embodiments, the anti-IL31 antibody is purified using chromatography, such as size exclusion chromatography, ion exchange chromatography, Protein A column chromatography, hydrophobic interaction chromatography, and CHT chromatography.

[0102] The term "companion animal species" refers to animals suitable for being companions to humans. In some embodiments, companion animal species are small mammals, such as canines, felines, dogs, cats, horses, rabbits, ferrets, guinea pigs, rodents, etc. In some embodiments, companion animal species are horses, cows, pigs, and other livestock.

[0103] The term "IL31 signaling function" refers to any one or combination of downstream activities that occur upon binding of IL31 to its receptor or receptor complex. In some embodiments, the IL31 signaling function comprises activation of Janus kinase (Jak) 1 or Jak2 signaling molecules. In some embodiments, the IL31 signaling function comprises phosphorylation of STAT-3 or STAT-5 proteins. In some embodiments, the IL31 signaling function comprises activating the ERK1 / 2 MAP kinase signaling pathway. In some embodiments, the IL31 signaling function comprises activating the PI3K / AKT signaling pathway. In some embodiments, the IL31 signaling function comprises activating the Jak1 / 2 signaling pathway.

[0104] "STAT phosphorylation" refers to the alteration of STAT proteins by phosphorylation after their expression. For example, "STAT-3 phosphorylation" refers to the phosphorylation of STAT-3, and "STAT-5 phosphorylation" refers to the phosphorylation of STAT-5. In some embodiments, STAT-3 phosphorylation is measured by immunoblot analysis. For example, cells (e.g., canine monocytic DH82 cells) are cultured at 1 x 10 ng / ml in a growth medium (e.g., MEM, Life Technologies®) containing 15% heat-inactivated fetal bovine serum, 2 mmol / L GlutaMax, 1 mmol / L sodium pyruvate, and 10 nM / mL canine interferon-c (R&D Systems, Minneapolis, MN, USA). 5Cells were seeded into 96-well cell culture plates at a density of 1000 cells / well in the presence of an anti-IL31 antibody described herein for 24 hours at 37°C. Immunoblot analysis of cell lysates using anti-phospho-STAT-3 and anti-STAT-3 antibodies (R&D Systems) was used to detect the relative levels of phosphorylated and non-phosphorylated STAT-3 relative to each other and compared to a β-actin control. Those skilled in the art will understand how to determine qualitative or quantitative protein concentrations by immunoblot. In some embodiments, relative concentrations are determined qualitatively by visual inspection of the immunoblot. In some embodiments, the levels of phosphorylated and non-phosphorylated STAT-3 are determined quantitatively by digitally imaging the immunoblot, determining the intensity of the bands, and back-calculating the concentration of phosphorylated or non-phosphorylated STAT-3 in the sample using a linear standard line of known concentrations of STAT-3 protein.

[0105] "Reduce" or "inhibit" means to decrease, reduce, or stop an activity, function, or amount compared to a reference. In some embodiments, "reduce" or "inhibit" refers to the ability to cause a 20% or greater overall decrease. In some embodiments, "reduce" or "inhibit" refers to the ability to cause a 50% or greater overall decrease. In some embodiments, "reduce" or "inhibit" refers to the ability to cause a 75%, 85%, 90%, 95%, or greater overall decrease. In some embodiments, the amount is inhibited or reduced over a period of time compared to a control dose (such as a placebo) over the same period of time. "Reference," as used herein, refers to any sample, standard, or level used for comparison purposes. A reference may be obtained from a healthy or non-diseased sample. In some examples, a reference is obtained from a non-diseased or untreated sample from a companion animal. In some examples, a reference is obtained from one or more healthy animals of a particular species that are not the animal being tested or treated.

[0106] The term "substantially reduced," as used herein, refers to a degree of reduction between a numerical value and a reference numerical value that is high enough that one of skill in the art would consider the difference between the two values ​​to be statistically significant within the context of the biological property measured by the numerical value and the reference numerical value. In some embodiments, a substantially reduced numerical value is greater than about any of a 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 60%, 70%, 80%, 90%, or 100% reduction compared to the reference value.

[0107] In some embodiments, the IL31 antibody may reduce IL31 signaling function in the companion animal species by at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, or 100% compared to IL31 signaling function in the absence of the antibody, as measured by a reduction in STAT-3 phosphorylation.In some embodiments, the reduction in IL31 signaling function or the reduction in STAT-3 phosphorylation is 10% to 15%, 10% to 20%, 10% to 25%, 10% to 30%, 10% to 35%, 10% to 40%, 10% to 45%, 10% to 50%, 10% to 60%, 10% to 70%, 10% to 80%, 10% to 90%, 10% to 100%, 15% to 20%, 15% to 25%, 15% to 30%, 15% to 35%, 15% to 40%, 15% to 45%, 15%~50%, 15%~60%, 15%~70%, 15%~80%, 15%~90%, 15%~100%, 20%~25%, 20%~30%, 20%~35%, 20%~40%, 20%~45%, 20%~50%, 20%~60%, 20%~70%, 20%~80%, 20%~90%, 20%~100%, 25%~30%, 25%~35%, 25%~40%, 25%~45%, 25%~50%, 25%~60%, 25%~70%, 25%~ 80%, 25%~90%, 25%~100%, 30%~35%, 30%~40%, 30%~45%, 30%~50%, 30%~60%, 30%~70%, 30%~80%, 30%~90%, 30%~100%, 35%~40%, 35%~45%, 35%~50%, 35%~60%, 35%~70%, 35%~80%, 35%~90%, 35%~100%, 40%~45%, 40%~50%, 40%~60%, 40%~70%, 40%~80 %, 40% to 90%, 40% to 100%, 45% to 50%, 45% to 60%, 45% to 70%, 45% to 80%, 45% to 90%, 45% to 100%, 50% to 60%, 50% to 70%, 50% to 80%, 50% to 90%, 50% to 100%, 60% to 70%, 60% to 80%, 60% to 90%, 60% to 100%, 70% to 80%, 70% to 90%, 70% to 100%, 80% to 90%, 80% to 100%, or 90% to 100%.

[0108] Pharmaceutical Compositions The terms "pharmaceutical formulation" and "pharmaceutical composition" mean a preparation that is in a form that allows the biological activity of the active ingredient to be effective and that does not contain additional components that are unacceptably toxic to the subject to which the formulation is administered.

[0109] "Pharmaceutically acceptable carrier" means a non-toxic solid, semi-solid, or liquid filler, diluent, encapsulating material, formulation aid, or carrier conventional in the art for use with therapeutic agents that together comprise a "pharmaceutical composition" for administration to a subject. A pharmaceutically acceptable carrier is non-toxic to a recipient at the dosage and concentration employed and is compatible with other ingredients of the formulation. A pharmaceutically acceptable carrier is suitable for the formulation employed. Examples of pharmaceutically acceptable carriers include alumina, aluminum stearate, lecithin, serum proteins such as human serum albumin, canine or other animal albumin, buffers such as phosphate, citrate, tromethamine, or HEPES buffer, glycine, sorbic acid, potassium sorbate, a partial glyceride mixture of saturated vegetable fatty acids, water, protamine sulfate, salts or electrolytes such as disodium hydrogen phosphate, potassium hydrogen phosphate, sodium chloride, zinc salts, colloidal silica, or magnesium trisilicate, polyvinylpyrrolidone, cellulose-based substances, polyethylene glycol, sucrose, mannitol, or amino acids including, but not limited to, arginine.

[0110] The pharmaceutical composition can be stored in lyophilized form. Thus, in some embodiments, the preparation process includes a lyophilization step. The lyophilized composition can then be re-formulated prior to administration to dogs, cats, or horses, typically as an aqueous composition suitable for parenteral administration. In other embodiments, particularly when the antibody is highly stable to thermal and oxidative denaturation, the pharmaceutical composition can be stored as a liquid, i.e., as an aqueous composition that can be administered directly or after appropriate dilution to dogs, cats, or horses. The lyophilized composition can be reconstituted with sterile water for injection (WFI). An antimicrobial agent (e.g., a bacteriostatic agent such as benzyl alcohol) may also be included. Thus, the present invention provides pharmaceutical compositions in solid or liquid form.

[0111] The pH of the pharmaceutical composition may range from about pH 5 to about pH 8 upon administration. The compositions of the present invention are sterile when used for therapeutic purposes. Sterility can be achieved by any of several means known in the art, including filtration through sterile filtration membranes (e.g., 0.2 micron membranes). Sterility may be maintained in the presence or absence of antibacterial agents.

[0112] In some embodiments, the pharmaceutically acceptable carrier or pharmaceutical composition has a pH of 5.0 to 6.2, 5.0 to 6.0, or 5.3 to 5.7. In some embodiments, the pharmaceutically acceptable carrier or pharmaceutical composition has a pH of 5.0, 5.1, 5.2, 5.3, 5.4, 5.5, 5.6, 5.7, 5.8, 5.9, 6.0, 6.1, or 6.2.

[0113] In some embodiments, the pharmaceutically acceptable carrier or pharmaceutical composition comprises L-histidine, sodium chloride, and polysorbate 80.

[0114] In some embodiments, the pharmaceutically acceptable carrier or pharmaceutical composition comprises sodium chloride at a concentration of 80 nM to 200 nM, 100 nM to 180 nM, 100 nM to 175 nM, 110 nM to 170 nM, 120 nM to 160 nM, 120 nM to 150 nM, 130 nM to 150 nM, 130 nM to 160 nM, 100 nM, 80 nM, 110 nM, 120 nM, 130 nM, 140 nM, 150 nM, 160 nM, 170 nM, 180 nM, or 200 nM.

[0115] In some embodiments, the pharmaceutically acceptable carrier or pharmaceutical composition comprises polysorbate 80 at a concentration of 0.005 mg / mL to 0.5 mg / mL, 0.01 mg / mL to 0.1 mg / mL, 0.1 mg / mL to 0.5 mg / mL, 0.005 mg / mL to 0.01 mg / mL, 0.1 mg / mL, 0.2 mg / mL, 0.3 mg / mL, 0.4 mg / mL, 0.05 mg / mL, 0.06 mg / mL, 0.07 mg / mL, 0.08 mg / mL, 0.09 mg / mL, or 0.1 mg / mL.

[0116] In some embodiments, the pharmaceutically acceptable carrier or pharmaceutical composition comprises L-histidine at a concentration of 5 mM to 100 mM, 10 mM to 50 mM, 20 mM to 30 mM, 10 mM to 30 mM, 20 mM to 80 mM, 30 mM to 70 mM, 40 mM to 60 mM, 10 mM, 15 mM, 20 mM, 25 mM, 30 mM, 40 mM, or 50 mM.

[0117] In some embodiments, the pharmaceutically acceptable carrier or pharmaceutical composition comprises m-cresol or benzyl alcohol. In some embodiments, the concentration of m-cresol is about 0.2%, about 0.1% to about 0.3%, about 0.08% to about 0.25%, or about 0.05% to about 0.25%. In some embodiments, the concentration of benzyl alcohol is about 1%, about 0.5% to about 2%, about 0.2% to about 2.5%, about 1% to about 5%, about 0.5% to about 5%, or about 1% to about 3%.

[0118] In some embodiments, the pharmaceutically acceptable carrier or pharmaceutical composition comprises a sugar. In some embodiments, the sugar is sucrose, trehalose, D-mannitol, maltose, and / or sorbitol. In some embodiments, the pharmaceutically acceptable carrier or pharmaceutical composition comprises a sugar at a concentration of 0.5% to 20%, 1% to 10%, 1% to 5%, 1% to 3%, 0.5%, 1%, 2%, 3%, 4%, 5%, or 10%.

[0119] In some embodiments, the pharmaceutically acceptable carrier or pharmaceutical composition comprises an antibacterial agent. In some embodiments, the pharmaceutically acceptable carrier or pharmaceutical composition comprises m-cresol or methylparaben. In some embodiments, the pharmaceutically acceptable carrier or pharmaceutical composition comprises 0.2% m-cresol. In some embodiments, the pharmaceutically acceptable carrier or pharmaceutical composition comprises 0.9% methylparaben.

[0120] Uses of antibodies and pharmaceutical compositions The antibodies of the present invention or pharmaceutical compositions comprising the antibodies may be useful for treating IL31-induced conditions. As used herein, "IL31-induced conditions" refers to diseases associated with, caused by, or characterized by elevated levels or altered gradients of IL31 concentration. Such IL31-induced conditions include, but are not limited to, pruritic diseases or allergic diseases. In some embodiments, the IL31-induced condition is atopic dermatitis, pruritus, asthma, psoriasis, scleroderma, or eczema. IL31-induced conditions may occur in companion animals, including, but not limited to, dogs, cats, or horses.

[0121] As used herein, "treatment" refers to an approach to obtain beneficial or desired clinical results. As used herein, "treatment" encompasses any administration or application of disease therapy in mammals, including companion animals. For purposes of this disclosure, beneficial or desired clinical results include, but are not limited to, any one or more of the following: alleviation of one or more symptoms; reduction in the extent of disease; prevention or delay of disease spread; prevention or delay of disease recurrence; delay or slowing of disease progression; amelioration of disease state; prevention of disease or disease progression; prevention or slowing of disease or its progression; halting its progression; and remission (partial or complete). Reduction of the pathological consequences of proliferative diseases is also encompassed by "treatment." The methods provided herein contemplate any one or more of these aspects of treatment. Consistent with the above, the term "treatment" does not require 100% elimination of all aspects of the disorder.

[0122] In some embodiments, anti-IL31 antibodies or pharmaceutical compositions comprising same can be utilized according to the methods herein for treating IL31-induced conditions, hi some embodiments, the anti-IL31 antibodies or pharmaceutical compositions are administered to companion animals, such as dogs, cats, or horses, to treat IL31-induced conditions.

[0123] A "therapeutically effective amount" of a substance / molecule, agonist, or antagonist can vary depending on factors such as the type of disease to be treated, the condition, the severity and course of the disease, the type of therapeutic objective, previous treatments, if any, medical history, response to previous treatments, the discretion of the attending veterinarian, the age, sex, and weight of the animal, and the ability of the substance / molecule, agonist, or antagonist to elicit a desired response from the animal. A therapeutically effective amount is also an amount in which the therapeutically beneficial effects of the substance / molecule, agonist, or antagonist outweigh any toxic or detrimental effects. A therapeutically effective amount may be delivered in one or more administrations. A therapeutically effective amount means an amount effective to achieve the desired therapeutic or prophylactic result, at the dosages and for the duration necessary.

[0124] In some embodiments, the anti-IL31 antibody or pharmaceutical composition comprising an anti-IL31 antibody is administered parenterally, subcutaneously, by intravenous infusion, or by intramuscular injection. In some embodiments, the anti-IL31 antibody or pharmaceutical composition comprising an anti-IL31 antibody is administered as a bolus injection or by continuous infusion over a period of time. In some embodiments, the anti-IL31 antibody or pharmaceutical composition comprising an anti-IL31 antibody is administered intramuscularly, intraperitoneally, intracerebrospinal, subcutaneously, intraarterially, intrasynovially, intrathecally, or by inhalation.

[0125] The anti-IL31 antibodies described herein may be administered in an amount ranging from 0.1 mg / kg to 100 mg / kg of body weight per dose. In some embodiments, the anti-IL31 antibodies may be administered in an amount ranging from 0.5 mg / kg to 50 mg / kg of body weight per dose. In some embodiments, the anti-IL31 antibodies may be administered in an amount ranging from 1 mg / kg to 10 mg / kg of body weight per dose. In some embodiments, the anti-IL31 antibody may be administered in an amount ranging from 0.5 mg / kg to 100 mg / kg body weight, from 1 mg / kg to 100 mg / kg body weight, from 5 mg / kg to 100 mg / kg body weight, from 10 mg / kg to 100 mg / kg body weight, from 20 mg / kg to 100 mg / kg body weight, from 50 mg / kg to 100 mg / kg body weight, from 1 mg / kg to 10 mg / kg body weight, from 5 mg / kg to 10 mg / kg body weight, from 0.5 mg / kg to 10 mg / kg body weight, or from 5 mg / kg to 50 mg / kg body weight.

[0126] The anti-IL31 antibody or pharmaceutical composition comprising the anti-IL31 antibody can be administered to the companion animal once or over a series of treatments. For example, the anti-IL31 antibody or pharmaceutical composition comprising the anti-IL31 antibody may be administered at least once, more than once, at least twice, at least three times, at least four times, or at least five times.

[0127] In some embodiments, the dose is administered once a week for at least two or three consecutive weeks, and in some embodiments, this treatment cycle is repeated two or more times, optionally separated by one or more weeks of no treatment. In other embodiments, the therapeutically effective dose is administered once a day for two to five consecutive days, and in some embodiments, this treatment cycle is repeated two or more times, optionally separated by one or more weeks of no treatment.

[0128] Administration "in combination with" one or more additional therapeutic agents includes simultaneous (concurrent) administration and consecutive or sequential administration in any order. The term "concurrently" is used herein to refer to the administration of two or more therapeutic agents where at least a portion of the administration overlaps in time, or where the administration of one therapeutic agent occurs within a close period of the administration of another therapeutic agent. For example, two or more therapeutic agents are administered with a temporal separation of about a specified number of minutes or less. The term "sequentially" is used herein to refer to the administration of two or more therapeutic agents where the administration of one or more agents continues after the administration of one or more other agents is discontinued, or where the administration of one or more agents begins before the administration of one or more other agents. For example, the administration of two or more therapeutic agents is separated in time by more than about a specified number of minutes. As used herein, "in combination with" refers to the administration of one treatment modality in addition to another treatment modality. Thus, "in combination with" refers to the administration of one treatment modality before, during, or after the administration of another treatment modality to an animal.

[0129] In some embodiments, the method comprises administering a Jak inhibitor, a PI3K inhibitor, an AKT inhibitor, or a MAPK inhibitor in combination with an anti-IL31 antibody or a pharmaceutical composition comprising an anti-IL31 antibody. In some embodiments, the method comprises administering an anti-IL17 antibody, an anti-TNFα antibody, an anti-CD20 antibody, an anti-CD19 antibody, an anti-CD25 antibody, an anti-IL4 antibody, an anti-IL13 antibody, an anti-IL23 antibody, an anti-IgE antibody, an anti-CD11α antibody, an anti-IL6R antibody, an anti-α4-integrin antibody, an anti-IL12 antibody, an anti-IL1β antibody, or an anti-BlyS antibody in combination with an anti-IL31 antibody or a pharmaceutical composition comprising an anti-IL31 antibody.

[0130] Provided herein are methods of exposing cells to an anti-IL31 antibody or a pharmaceutical composition comprising an anti-IL31 antibody under conditions that allow binding of the antibody to IL31. In some embodiments, the cells are exposed to the antibody or pharmaceutical composition ex vivo. In some embodiments, the cells are exposed to the antibody or pharmaceutical composition in vivo. In some embodiments, the cells are exposed to the anti-IL31 antibody or pharmaceutical composition under conditions that allow binding of the antibody to intracellular IL31. In some embodiments, the cells are exposed to the anti-IL31 antibody or pharmaceutical composition under conditions that allow binding of the antibody to extracellular IL31. In some embodiments, the cells may be exposed to the anti-IL31 antibody or pharmaceutical composition in vivo by any one or more of the administration methods described herein, including but not limited to, intraperitoneal, intramuscular, or intravenous injection into a subject. In some embodiments, the cells may be exposed to the anti-IL31 antibody or pharmaceutical composition ex vivo by exposing the cells to medium containing the antibody or pharmaceutical composition. In some embodiments, the permeability of the cell membrane can be affected by using any number of methods understood by one of skill in the art (e.g., subjecting the cells to electroporation or exposing the cells to a solution containing calcium chloride) prior to exposing the cells to a medium containing the antibody or pharmaceutical composition.

[0131] In some embodiments, binding reduces IL31 signaling function in the cell, hi some embodiments, the IL31 antibody may reduce IL31 signaling function in the cell by at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, or 100% compared to IL31 signaling function in the absence of the antibody, as measured by a reduction in STAT-3 phosphorylation.In some embodiments, the reduction in IL31 signaling function or the reduction in STAT-3 phosphorylation is 10% to 15%, 10% to 20%, 10% to 25%, 10% to 30%, 10% to 35%, 10% to 40%, 10% to 45%, 10% to 50%, 10% to 60%, 10% to 70%, 10% to 80%, 10% to 90%, 10% to 100%, 15% to 20%, 15% to 25%, 15% to 30%, 15% to 35%, 15% to 40%, 15% to 45%, 15%~50%, 15%~60%, 15%~70%, 15%~80%, 15%~90%, 15%~100%, 20%~25%, 20%~30%, 20%~35%, 20%~40%, 20%~45%, 20%~50%, 20%~60%, 20%~70%, 20%~80%, 20%~90%, 20%~100%, 25%~30%, 25%~35%, 25%~40%, 25%~45%, 25%~50%, 25%~60%, 25%~70%, 25%~ 80%, 25%~90%, 25%~100%, 30%~35%, 30%~40%, 30%~45%, 30%~50%, 30%~60%, 30%~70%, 30%~80%, 30%~90%, 30%~100%, 35%~40%, 35%~45%, 35%~50%, 35%~60%, 35%~70%, 35%~80%, 35%~90%, 35%~100%, 40%~45%, 40%~50%, 40%~60%, 40%~70%, 40%~80 %, 40% to 90%, 40% to 100%, 45% to 50%, 45% to 60%, 45% to 70%, 45% to 80%, 45% to 90%, 45% to 100%, 50% to 60%, 50% to 70%, 50% to 80%, 50% to 90%, 50% to 100%, 60% to 70%, 60% to 80%, 60% to 90%, 60% to 100%, 70% to 80%, 70% to 90%, 70% to 100%, 80% to 90%, 80% to 100%, or 90% to 100%.

[0132] Provided herein are methods using anti-IL31 antibodies, polypeptides, and polynucleotides for the detection, diagnosis, and monitoring of IL31-induced conditions. Provided herein are methods for determining whether a companion animal will respond to anti-IL31 antibody treatment. In some embodiments, the method comprises detecting whether the animal has cells that express IL31 using an anti-IL31 antibody. In some embodiments, the detection method comprises contacting a sample with the antibody, polypeptide, or polynucleotide and determining whether the degree of binding differs from that of a reference or comparison sample (e.g., a control). In some embodiments, the method may be useful for determining whether the antibodies or polypeptides described herein are an appropriate treatment for a subject animal.

[0133] In some embodiments, the sample is a biological sample. The term "biological sample" refers to a quantity of material from a living organism or formerly living organism. In some embodiments, the biological sample is a cell or cell / tissue lysate. In some embodiments, biological samples include, but are not limited to, blood (e.g., whole blood), plasma, serum, urine, synovial fluid, and epithelial cells.

[0134] In some embodiments, cells or cell / tissue lysates are contacted with an anti-IL31 antibody and binding between the antibody and the cells is determined. If the test cells exhibit binding activity compared to reference cells of the same tissue type, this indicates that the subject would benefit from treatment with the anti-IL31 antibody. In some embodiments, the test cells are from tissues of companion animals.

[0135] Various methods known in the art for detecting specific antibody-antigen binding can be used. Exemplary immunoassays that can be performed include fluorescence polarization immunoassay (FPIA), fluorescence immunoassay (FIA), enzyme immunoassay (EIA), turbidimetric inhibition immunoassay (NIA), enzyme-linked immunosorbent assay (ELISA), and radioimmunoassay (RIA). Indicator moieties, or label groups, can be attached to the antibody of interest and are selected to meet the needs of the various methods used, which are often determined by the availability of assay equipment and compatible immunoassay procedures. Suitable labels include, but are not limited to, radionuclides (e.g., 125 I, 131 I, 35 S, 3 H, or 32 P), enzymes (e.g., alkaline phosphatase, horseradish peroxidase, luciferase, or p-galactosidase), fluorescent moieties or proteins (e.g., fluorescein, rhodamine, phycoerythrin, GFP, or BFP), or luminescent moieties (e.g., Qdot™ nanoparticles supplied by Quantum Dot Corporation, Palo Alto, Calif.). General techniques used in performing the various immunoassays described above are known to those of skill in the art.

[0136] For diagnostic purposes, polypeptides, including antibodies, can be labeled with a detectable moiety, including, but not limited to, radioisotopes, fluorescent labels, and various enzyme-substrate labels known in the art. Methods for conjugating labels to antibodies are known in the art. In some embodiments, the anti-IL31 antibody need not be labeled, and its presence can be detected using a second, labeled antibody that binds to the first anti-IL31 antibody. In some embodiments, the anti-IL31 antibody can be employed in any known assay method, such as competitive binding assays, direct and indirect sandwich assays, and immunoprecipitation assays. Zola, Monoclonal Antibodies: A Manual of Techniques, pp. 147-158 (CRC Press, Inc., 1987). Anti-IL31 antibodies and polypeptides can also be used in in vivo diagnostic assays, such as in vivo imaging. Generally, the antibody or polypeptide is conjugated to a radionuclide (e.g., a fluorophore) so that the location of cells or tissues of interest can be identified using immunoscintigraphy. 111 In, 99 Tc, 14 C. 131 I, 125 I, 3 H, or any other radionuclide label, including those outlined herein. Antibodies are also used as staining agents in pathology using techniques well known in the art.

[0137] In some embodiments, the first antibody is used for diagnosis and the second antibody is used for treatment. In some embodiments, the first antibody and the second antibody are different. In some embodiments, the first antibody and the second antibody bind to different epitopes, thereby allowing both to bind to the antigen simultaneously.

[0138] The following examples illustrate certain aspects of the present disclosure and are not intended to limit the disclosure in any way. [Example]

[0139] Example 1: Identification of mouse monoclonal antibodies that bind to canine IL31 The canine IL31 gene encoding the IL31 protein (SEQ ID NO: 22) was synthesized with a C-terminal poly-His tag and cloned into a mammalian expression vector. The plasmid carrying the canine IL31 gene was transfected into 293 cells.

[0140] The supernatant containing canine IL31 protein was collected and filtered. Canine IL31 was affinity purified using a Ni-NTA column (CaptivA® Protein A Affinity Resin, Repligen).

[0141] Mouse monoclonal antibodies were identified using standard immunization with canine IL31 produced by 293 cells as the immunogen. Monoclonal antibodies were obtained by standard hybridoma technology using two different adjuvants during immunization (Antibody Solutions, Sunnyvale, CA). To screen for clones producing IL31-binding antibodies, an enzyme-linked immunosorbent assay (ELISA) was developed. Canine IL31 was first biotinylated and then introduced into streptavidin-coated wells. Immunized serum was then added to the wells, washed, and detected with an HRP-conjugated anti-mouse antibody. The presence of canine IL31-binding antibodies generated a positive signal. Of the thousands of clones tested by ELISA, 170 clones with the highest binding affinity were selected based on signal intensity and further tested by biosensor assay (Forte Bio Octet). Biotinylated canine IL31 was bound to a sensor chip, and the supernatants of hybridoma clones containing anti-canine IL31 antibodies were selected based on their slow off-rates (the rate of dissociation between the antibody and the ligand). The binding affinities of the top 19 candidates were measured at a single concentration and reported as equilibrium dissociation constants (Kd) after measuring the antibody concentration by a Protein A titration assay using a Biosensor Octet. The Kd values ​​of the top 19 candidates were each less than 10 nM.

[0142] Furthermore, each of the 170 clones with high binding activity based on ELISA was tested for neutralizing activity. To evaluate the activity of the top candidates in reducing canine IL31-mediated pSTAT signaling using canine DH82 cells, a cell-based functional assay, described below in Example 4, was performed. The top four clones (M14, M18, M19, and M87) were selected for further study. Notably, the majority of high-affinity binders identified by ELISA were not functional.

[0143] Example 2: Identification of DNA sequences encoding VH and VL of monoclonal antibodies Hybridoma cells producing M14, M18, M19, and M87 were pelleted. RNA was extracted, and cDNA was obtained using standard techniques with oligonucleotide primers to amplify mouse immunoglobulin (Ig) variable domains. The variable heavy (VH) and variable light (VL) chains of each of the four clones were sequenced and analyzed by sequence alignment (Figure 1, SEQ ID NOS: 36-43). Notably, three of the four active antibodies (M14, M18, and M19) share the same six CDR sequences, with the exception that CDR-L1 of M18 has an isoleucine at position 14 (SEQ ID NOS: 63), while M14 and M19 have a methionine at position 14 (SEQ ID NOS: 8); and CDR-H2 of M18 has a tyrosine at position 9 (SEQ ID NOS: 62 and 87), while M14 and M19 have an aspartic acid at position 14 (SEQ ID NOS: 2 and 89). The similarity of the CDR sequences suggests that M14, M18, and M19 share a common epitope.

[0144] Example 3: Expression and purification of mouse-canine chimeric and caninized IL31mAb M14 from CHO cells A DNA sequence encoding a chimeric antibody was designed to fuse the murine M14 VH (SEQ ID NO: 25) and murine VL (SEQ ID NO: 24) to a canine constant heavy chain and a canine constant light chain. The nucleotide sequence was chemically synthesized and inserted into an expression vector suitable for transfection into CHO host cells. After transfection into CHO cells, the light chain or heavy chain protein, or both, was secreted from the cells. For example, chimeric M14 using canine IgG-B was purified by a single-step Protein A column chromatography.

[0145] The mouse M14 VH and VL were caninized by searching and selecting the correct canine germline antibody sequence as a template for CDR grafting and performing protein modeling. The caninized M14 IgG-B (SEQ ID NO: 18 and SEQ ID NO: 21) was easily expressed and purified in a single step by a Protein A column or other chromatography method, such as ion exchange column chromatography, hydrophobic interaction column chromatography, mixed-mode column chromatography such as CHT, or multimodal mode column chromatography such as CaptoMMC. Low pH or other virus inactivation and virus removal steps can be applied. The purified protein is mixed with excipients and filter-sterilized to prepare the pharmaceutical composition of the present invention. The pharmaceutical composition is administered to dogs with atopic dermatitis in an amount sufficient to bind to and inhibit IL31.

[0146] The vector was then used to perform pilot-scale transfections in CHO-S cells using FreestyleMax™ transfection reagent (Life Technologies). The supernatant was harvested by clarifying the conditioned medium. The protein was purified by a single-pass Protein A chromatography step and used for further studies.

[0147] Example 4: Demonstration of IL31 binding activity This example demonstrates that antibodies of the invention, designated chimeric M14 (SEQ ID NO: 26 and SEQ ID NO: 27) and caninized M14 (SEQ ID NO: 18 and SEQ ID NO: 21), bind to canine IL31 with the kinetics required for therapeutic activity.

[0148] Binding analysis was performed using the Octet biosensor as follows. Briefly, canine IL31 was biotinylated at primary amine groups using EZ-Link™ NHS-LC-Biotin (ThermoScientific, Cat. No. 21336) or at glycan groups using EZ-Link™ Biotin-LC-Hydrazide (ThermoFisher Scientific, Cat. No. 21340) according to the manufacturer's instructions. Free, unreacted biotin was removed from biotinylated IL31 by extensive dialysis. Biotinylated canine IL31 was captured on a streptavidin sensor chip. Association of chimeric and caninized M14 antibodies at four different concentrations (400, 200, 66.6, and 33 nM) with human and canine IL31 (amine-conjugated-biotin), and caninized M14 antibodies at 100 nM with canine IL31 (glycan-conjugated-biotin) was monitored for 90 seconds. Dissociation was monitored for 600 seconds. A buffer-only blank curve was subtracted to correct for any drift. Data were analyzed using k on , k off The data were fitted to a 2:1 binding model using ForteBio™ data analysis software to determine kd. The buffer for dilution and all binding steps was 20 mM phosphate, 150 mM NaCl, pH 7.2.

[0149] Canine IL31 with a C-terminal poly-His tag was expressed in CHO-S cells and purified. Human IL31 was obtained from Sino Biological, and a streptavidin biosensor was obtained from ForteBio (Cat. #18-509). The binding kinetics were as follows: For the ligand canine IL31 (amine-conjugated biotin), the Kd (M) of chimeric M14 was <1.0 × 10 -11(Fig. 2), and <1.0 × 10 -11 For the ligand canine IL31 (glycan-conjugated biotin), the Kd (M) of caninized M14 was <1.0 × 10 -12 , k off (1 / s) is <1.0×10 -7 It was.

[0150] Chimeric M14 and caninized M14 had no apparent binding signal to human IL31, therefore, Kd could not be determined.

[0151] Example 5: Demonstration that M14 inhibits canine IL31 signaling After binding to its IL31 receptor, IL31 activates Janus kinase (Jak) 1 and Jak2 signaling molecules. Subsequently, activated Jaks stimulate the phosphorylation of downstream signaling proteins STAT-3 and STAT-5. Anti-phospho-Stat3 immunoblot analysis was used to detect anti-IL31 activity from protein A-purified fractions of cell-free media (Gonzales et al., Vet Dermatol, 2013, 24, 48-e12). Briefly, canine monocytic DH82 cells (American Type Culture Collection, Manassas, VA, USA) were cultured at 1 × 10 per well in MEM growth medium (Life Technologies) containing 15% heat-inactivated fetal bovine serum, 2 mmol / L GlutaMax, 1 mmol / L sodium pyruvate, and 10 ng / mL canine interferon-c (R&D Systems, Minneapolis, MN, USA). 5Cells were seeded into 96-well flat-bottom cell culture plates at a density of 1000 kJ / mL (8 nM) and incubated at 37°C for 24 hours. In this experiment, the concentration of canine IL31-Fc was 5 ng / mL (8 nM). Anti-phospho-STAT-3 and anti-STAT-3 antibodies were purchased from R&D Systems. Anti-β-actin antibody was obtained from Sigma-Aldrich. As shown in Figure 4, canine IL31 signaling (as evidenced by reduced STAT-3 phosphorylation) decreased with increasing concentrations of caninized M14 exposed to cells (lane 1: no anti-IL31 antibody, lane 2: 3.3 nM, lane 3: 6.6 nM, lane 4: 9.9 nM, and lane 5: 13.2 nM).

[0152] Example 6: Identification of M14 canine IL31 binding epitopes To identify the canine IL31 epitope recognized by M14, we generated multiple GST-canine IL31 fragment fusion molecules and expressed the proteins in Escherichia coli (E. coli). After transferring the GST fusion proteins to a membrane, the membrane was probed with chimeric M14. A positive signal was obtained when the IL31 fragment contained the epitope.

[0153] Combining Figures 5 and 6 demonstrates that M14 can recognize the smallest fragment (SEQ ID NO: 23).

[0154] Example 7: Demonstration that M14 cross-reacts with feline IL31 To examine whether the M14 antibody recognizes feline IL31 (SEQ ID NO: 28) or equine IL31 (SEQ ID NO: 29), each protein was fused to human Fc and expressed in mammalian 293 cells. Partially purified proteins were blotted onto membranes and probed with M14 antibody. The immunoblot shown in Figure 1 demonstrates that M14 binds to feline IL31. The immunoblot assay did not detect binding of M14 to equine IL31. However, biolayer interferometry analysis revealed that the M14 antibody binds to equine IL31, albeit with low affinity. Preliminary Kd measurements using biotinylated equine IL31 immobilized on a sensor revealed an affinity (Kd) of approximately 10-50 nM.

[0155] Example 8: Feline M14 The M14 variable light chain was felineized as (SEQ ID NO: 32), and the M14 variable heavy chain was felineized as (SEQ ID NO: 33). First, the mouse heavy and light chain variable sequences were used to search for the correct variants of the feline VH and VL. The correct feline frames were selected for CDR grafting. These were further optimized using structural modeling. The felineized VH and VL were fused to the feline IgG heavy chain constant domains (CH1, CH2, and CH3) and the feline light chain constant domain (CL1).

[0156] Feline M14 chimeric antibody (SEQ ID NO: 30 and SEQ ID NO: 31) or felineized M14 antibody (SEQ ID NO: 34 and SEQ ID NO: 35) can be administered to cats for the treatment of IL31-induced conditions.

[0157] Example 9: Identification of M14 canine IL31 binding epitopes To further identify the amino acid residues of the canine IL31 epitope recognized by M14, multiple GST canine IL31 epitope fragment fusion molecules with alanine mutations were expressed in E. coli. Figures 8 to 12 show immunoblots of fine epitope mapping of canine IL31-GST fusion proteins probed with anti-canine IL31 mAb (M14) or caninized M14 (upper panels) and an anti-GST antibody control (lower panels). The epitope fragments tested in each lane are listed below the immunoblot. The fragment names indicate the amino acid range of mature canine IL31 (SEQ ID NO: 44) tested and the position of the alanine mutation, if any. A positive signal was generated when the IL31 fragment contained the wild-type epitope, whereas a negative signal was generated when the IL31 fragment contained alanine substitutions at residues important for antibody-ligand interaction.

[0158] The results of the epitope mapping study are summarized in Table 3 below. The results suggest that P12, S13, D14, and K17 of mature canine IL31 (SEQ ID NO: 44) are involved in binding of antibody M14, and that R16 and I18 are partially involved in recognizing M14. Collectively, the results of this study suggest that the motif of the IL31 polypeptide that binds to the CDR of antibody M14 is PSDX1X2KI, where X1 and X2 are variable (SEQ ID NO: 45). Table 3. TIFF2025138636000014.tif68170

[0159] Example 10: M14 specifically binds to IL31 from other species that has the PSDX1X2KI epitope motif. The epitope motif of IL31 recognized by M14 (PSDX1X2KI, SEQ ID NO: 45) does not exist in the amino acid sequence of human (SEQ ID NO: 46) or mouse (SEQ ID NO: 61) IL31. However, this motif does not exist in the feline IL31. Domestic cat (Felis catus) (XP_011286140.1; SEQ ID NO: 28) Walrus (Odobenus rosmarus divergens) (XP_004395998.1; SEQ ID NO: 47) Papio Anubis (XP_003907358.1; SEQ ID NO: 49) Polar bear (Ursus maritimus) (XP_008687166.1; SEQ ID NO: 51) Weddell seal (Leptonychotes weddellii) (XP_006746595.1; SEQ ID NO: 52) Amur tiger (Panthera tigris altaica) (XP_007079636.1; SEQ ID NO: 53) Cheetah (Acinonyx jubatus) (XP_014919275.1; SEQ ID NO: 54) Cynomolgus monkey (Macaca fascicularis) (EHH66805.1; SEQ ID NO: 55) Rhesus monkey (Macaca mulatta) (EHH21279.1; SEQ ID NO: 56) Drill (Mandrillus leucophaeus) (XP_011819882.1; SEQ ID NO: 57) Green monkey (Chlorocebus sabaeus) (XP_008003211.1; SEQ ID NO: 58) Sooty mangabey (Cercocebus atys) (XP_011926625.1; SEQ ID NO: 59) Golden snub-nosed monkey (Rhinopithecus roxellana) (XP_010366647.1; SEQ ID NO: 60) It has been identified in several other species, including

[0160] Walrus IL31 (SEQ ID NO: 47) and Anubis baboon IL31 (SEQ ID NO: 49) possess the PSDX1X2KI epitope (SEQ ID NO: 45), which can be recognized by the antibody M14. To facilitate protein purification, a C-terminal His tag was added to walrus IL31 (SEQ ID NO: 48) and Anubis baboon IL31 (SEQ ID NO: 50). Western blot analysis confirmed that M14 binds to walrus IL31 (Figure 13). The M14 antibody or its derivatives can be used as therapeutic and diagnostic agents for diseases induced by IL31 in any of the species listed above.

[0161] Example 11: Thermal stability of caninized M14 antibody The thermal stability of the caninized M14 antibody was analyzed across a wide pH range in comparison with Zoetis' commercially available anti-IL31 antibody, CYTOPOINT™, using differential scanning fluorescence (DSF). The melting temperatures (Tm) of each antibody at various pHs are listed in Table 4 below. Both CYTOPOINT™ and caninized M14 antibody were buffer-exchanged into the assay buffers listed in Table 4 using a PD Minitrap™ G-25 column (GE Healthcare). The Tm of each antibody was assessed using the same buffer and protein concentration. The caninized M14 antibody demonstrated improved thermal stability compared to CYTOPOINT™ across a wide pH range. Furthermore, under stress conditions of 0.22 mg / ml antibody at 55°C for 2 days, CYTOPOINT™ precipitated, whereas no precipitation was observed with the caninized M14 antibody. Table 4. TIFF2025138636000015.tif95170

[0162] Example 12: Caninized M14 antibody buffer formulation The thermal stability of caninized M14 antibody in various buffer formulations was analyzed. Buffers containing sodium phosphate, sodium acetate, or L-histidine were considered. Other formulation variables included different pHs (pH 5.2, 5.5, 6.0, 6.5, and 7.0), different sodium chloride concentrations (50 mM and 140 mM), different polysorbates (polysorbate 20 and polysorbate 80), and different antimicrobial agents (m-cresol and methylparaben). The melting temperature (Tm) of the caninized M14 antibody in each buffer was measured using differential scanning fluorescence (DSF) from 20°C to 95°C. Table 5 lists the Tm values ​​of the caninized M14 antibody in the various buffers tested. Table 5 TIFF2025138636000016.tif224170TIFF2025138636000017.tif254170TIFF2025138636000018.tif254170TIFF2025138636000019.tif138170

[0163] The Tm of formulations D1, D2, D3, D4, D5, and D6 in the presence of each one of the following sugars (1%), sucrose, trehalose, D-mannitol, maltose, and sorbitol, was further tested using DSF.

[0164] Formulations in the presence and absence of sugars were also tested under stress conditions: 40°C for 1 day, followed by 45°C for 1 day, and then 55°C for 4 days. Size-exclusion HPLC analysis was used to detect and quantify the monomeric and aggregated forms of antibody in the various formulations after stress conditions. Samples were loaded onto a SHODEX™ KW803 column (8 mm x 300 mm) attached to a KW-G guard column. An Agilent 1100 chromatography system was used, with 2x PBS (270 mM NaCl, 5.4 mM KCl, 8.6 mM Na2PO4), pH 7.2, as the running buffer and a constant flow rate of 0.5 mL / min. The column was calibrated using BIORAD gel filtration standards (catalog no. 151-1901) consisting of thyroglobulin, bovine γ-globulin, chicken ovalbumin, equine myoglobin, and vitamin B12 (molecular weights ranging from 1,350 to 670,000). The amount of monomeric antibody remaining in solution was determined by measuring UV absorbance at 214 nm or 280 nm and calculating the peak area under the curve.

[0165] Based on DSF and HPLC analysis, formulations containing L-histidine, sodium chloride, polysorbate 80, and having a pH of 5.0 to 6.2, such as formulations D1, D2, D3, D4, D6, D7, D10, and D12, were considered more desirable. For example, a desirable formulation for single dosing is 20 mM L-histidine, 140 mM sodium chloride, polysorbate 80 (0.05 mg / mL), pH 5.5, and a desirable formulation for multiple dosing (in the presence of a preservative) is: 1.20 mM L-histidine, 140 mM sodium chloride, polysorbate 80 (0.05 mg / mL), sucrose (1–3%), m-cresol (0.2%), pH 5.5, and 2.20 mM L-histidine, 140 mM sodium chloride, polysorbate 80 (0.05 mg / mL), trehalose (1–3%), methylparaben (0.9%), pH 5.5 is.

Claims

1. An isolated antibody that binds to canine IL31, wherein the antibody binds to an epitope comprising the amino acid sequence of SEQ ID NO:

23.

2. An isolated antibody that binds to canine IL31, comprising PSDX １ X ２ An antibody that binds to an epitope comprising the amino acid sequence of KI (SEQ ID NO: 45), wherein X is any amino acid residue.

3. X １ is a hydrophobic amino acid, or X １ is selected from A, V, I, and L, or X １ is selected from V and I, and X ２ is a hydrophilic amino acid, or X ２ is selected from A, R, K, Q and N, or X ２ The isolated antibody of claim 2, wherein is selected from R and Q.

4. X １ is V and X ２ is R or X １ is I and X ２ The isolated antibody of claim 2 or claim 3, wherein is Q.

5. An antibody described in any one of claims 2 to 4, which binds to an epitope comprising the amino acid sequence of SEQ ID NO:

88.

6. 5 x 10 measured by biolayer interferometry －６ Less than M, 1 x 10 －６ Less than M, 5 x 10 －７ Less than M, 1 x 10 －７ Less than M, 5 x 10 －８ Less than M, 1 x 10 －８ Less than M, 5 x 10 －９ Less than M, 1 x 10 －９ Less than M, 5 x 10 －１０ Less than M, 1 x 10 －１０ Less than M, 5 x 10 －１１ Less than M, 1 x 10 －１１ Less than M, 5 x 10 －１２ Less than M or 1 x 10 －１２ The antibody of any one of claims 1 to 5, which binds to canine IL31 with a dissociation constant (Kd) of less than M.

7. 7. The antibody of any one of claims 1 to 6, which reduces IL31 signaling function in a companion animal species as measured by reduced phosphorylation of STAT-3.

8. The antibody of claim 7, wherein the companion animal species is a dog, a cat, or a horse.

9. 9. The antibody according to any one of claims 1 to 8, which binds to feline IL31 or equine IL31 as determined by immunoblot analysis and / or biolayer interferometry.

10. 10. The antibody of claim 1, which competes with monoclonal M14 antibody in binding to canine IL31.

11. The antibody according to any one of claims 1 to 10, which competes with the monoclonal M14 antibody in binding to feline IL31.

12. An antibody described in any one of claims 1 to 11, which does not bind to human IL31 as determined by immunoblot analysis and / or biolayer interferometry.

13. The antibody of any one of claims 1 to 12, which is a monoclonal antibody.

14. 14. The antibody of any one of claims 1 to 13, which is a canine, caninized, feline, felineized, equine, equine-derived antibody, or chimeric antibody.

15. 15. The antibody of any one of claims 1 to 14, which is a chimeric antibody comprising a murine variable heavy chain framework region or a murine variable light chain framework region.

16. comprising a heavy chain and a light chain, a) the heavy chain comprises a CDR-H1 sequence having at least 85%, at least 90%, at least 95%, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO: 1; a CDR-H2 sequence having at least 85%, at least 90%, at least 95%, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO: 2, 62, 89, or 87; and a CDR-H3 sequence having at least 85%, at least 90%, at least 95%, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO: 3; and b) the light chain comprises a CDR-L1 sequence having at least 85%, at least 90%, at least 95%, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO:8 or SEQ ID NO:63; a CDR-L2 sequence having at least 85%, at least 90%, at least 95%, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO:9; and a CDR-L3 sequence having at least 85%, at least 90%, at least 95%, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO:

10. An antibody described in any one of claims 1 to 15.

17. a) a heavy chain comprising: (i) a CDR-H1 comprising the amino acid sequence of SEQ ID NO: 1; (ii) a CDR-H2 comprising the amino acid sequence of SEQ ID NO: 2 or SEQ ID NO: 89; and (iii) a CDR-H3 comprising the amino acid sequence of SEQ ID NO: 3; or b) a heavy chain comprising: (i) a CDR-H1 comprising the amino acid sequence of SEQ ID NO: 1; (ii) a CDR-H2 comprising the amino acid sequence of SEQ ID NO: 62 or SEQ ID NO: 87; and (iii) a CDR-H3 comprising the amino acid sequence of SEQ ID NO:

3.

17. The antibody of any one of claims 1 to 16, comprising:

18. a) a light chain comprising: (i) a CDR-L1 comprising the amino acid sequence of SEQ ID NO: 8; (ii) a CDR-L2 comprising the amino acid sequence of SEQ ID NO: 9; and (iii) a CDR-L3 comprising the amino acid sequence of SEQ ID NO: 10; or b) a light chain comprising: (i) a CDR-L1 comprising the amino acid sequence of SEQ ID NO: 63; (ii) a CDR-L2 comprising the amino acid sequence of SEQ ID NO: 9; and (iii) a CDR-L3 comprising the amino acid sequence of SEQ ID NO:

10.

18. The antibody of any one of claims 1 to 17, comprising:

19. (a) a variable region heavy chain framework 1 (HC-FR1) sequence of SEQ ID NO: 4, 70, or 79; (b) a HC-FR2 sequence of SEQ ID NO: 5, 71, or 80; (c) a HC-FR3 sequence of SEQ ID NO: 6, 72, 73, or 81; (d) a HC-FR4 sequence of SEQ ID NO: 7, 74, or 82; (e) a variable region light chain framework 1 (LC-FR1) sequence of SEQ ID NO: 11, 75, or 83; (f) a LC-FR2 sequence of SEQ ID NO: 12, 76, or 84; (g) a LC-FR3 sequence of SEQ ID NO: 13, 77, or 85; or (h) a LC-FR4 sequence of SEQ ID NO: 14, 78, or 86.

19. The antibody of any one of claims 16 to 18, further comprising one or more of:

20. a) (i) a variable light chain sequence having at least 85%, at least 90%, at least 95%, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO: 24; (ii) a variable heavy chain sequence having at least 85%, at least 90%, at least 95%, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO: 25, or (iii) the variable light chain sequence of (i) and the variable heavy chain sequence of (ii); or b) (i) a variable light chain sequence having at least 85%, at least 90%, at least 95%, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO: 16; (ii) a variable heavy chain sequence having at least 85%, at least 90%, at least 95%, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO: 15; or (iii) the variable light chain sequence of (i) and the variable heavy chain sequence of (ii), or c) (i) a variable light chain sequence having at least 85%, at least 90%, at least 95%, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO: 32; (ii) a variable heavy chain sequence having at least 85%, at least 90%, at least 95%, or at least 98% sequence identity to the amino acid sequence of SEQ ID NO: 33; or (iii) the variable light chain sequence of (i) and the variable heavy chain sequence of (ii).

20. The antibody of any one of claims 1 to 19, comprising:

21. 21. The antibody of any one of claims 1 to 20, comprising the variable light chain sequence of SEQ ID NO: 24, SEQ ID NO: 16 or SEQ ID NO:

32.

22. 22. The antibody of any one of claims 1 to 21, comprising the variable heavy chain sequence of SEQ ID NO: 25, SEQ ID NO: 15 or SEQ ID NO:

33.

23. 23. The antibody of any one of claims 1 to 22, comprising a variable light chain sequence of SEQ ID NO: 24 and a variable heavy chain sequence of SEQ ID NO: 25, a variable light chain sequence of SEQ ID NO: 16 and a variable heavy chain sequence of SEQ ID NO: 15; or a variable light chain sequence of SEQ ID NO: 32 and a variable heavy chain sequence of SEQ ID NO:

33.

24. 24. The antibody of any one of claims 1 to 23, comprising a constant heavy chain region or a constant light chain region derived from a companion animal.

25. 25. The antibody of any one of claims 1 to 24, comprising: (a) a dog heavy chain constant region selected from an IgG-A, IgG-B, IgG-C, and IgG-D constant region; (b) a feline heavy chain constant region selected from an IgG1, IgG2a, and IgG2b constant region; or (c) an horse heavy chain constant region selected from an IgG1, IgG2, IgG3, IgG4, IgG5, IgG6, and IgG7 constant region.

26. a) (i) the light chain amino acid sequence of SEQ ID NO: 26; (ii) the heavy chain amino acid sequence of SEQ ID NO: 27; or (iii) the light chain amino acid sequence of (i) and the heavy chain amino acid sequence of (ii), or b) (i) the light chain amino acid sequence of SEQ ID NO: 30, (ii) the heavy chain amino acid sequence of SEQ ID NO: 31, or (iii) the light chain amino acid sequence of (i) and the heavy chain amino acid sequence of (ii), or c) (i) a light chain amino acid sequence of SEQ ID NO: 34; (ii) a heavy chain amino acid sequence of SEQ ID NO: 35; or (iii) a light chain amino acid sequence of (i) and a heavy chain amino acid sequence of (ii).

26. The antibody of any one of claims 1 to 25, comprising:

27. 27. The antibody of any one of claims 1 to 26, comprising the light chain amino acid sequence of SEQ ID NO:

21.

28. 28. The antibody of any one of claims 1 to 27, comprising the heavy chain amino acid sequence of SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19 or SEQ ID NO:

20.

29. Fv, scFv, Fab, Fab', F(ab') ２ 29. The antibody of any one of claims 1 to 28, which is an antibody fragment selected from Fab'-SH and Fab'-SH.

30. 30. The antibody of any one of claims 1 to 29, which is bispecific and binds to IL31 and one or more antigens selected from IL17, TNFα, CD20, CD19, CD25, IL4, IL13, IL23, IgE, CD11α, IL6R, α4-integrin, IL12, IL1β, or BlyS.

31. 31. An isolated nucleic acid encoding an antibody according to any one of claims 1 to 30.

32. 32. A host cell comprising the nucleic acid of claim 31.

33. 33. A method of producing an antibody, comprising culturing the host cell of claim 32 and isolating the antibody.

34. 31. A pharmaceutical composition comprising an antibody according to any one of claims 1 to 30 and a pharmaceutically acceptable carrier.

35. A pharmaceutical composition comprising an anti-IL31 antibody and a pharmaceutically acceptable carrier, wherein the pharmaceutically acceptable carrier comprises L-histidine, sodium chloride and polysorbate 80, and wherein the pharmaceutical composition has a pH of 5.0 to 6.

2.

36. 36. The pharmaceutical composition of claim 35, having a pH of 5.0 to 6.0, or 5.3 to 5.7, or 5.

5.

37. 37. The pharmaceutical composition of claim 35 or claim 36, having an L-histidine concentration of 5 mM to 100 mM, 10 mM to 50 mM, 20 mM to 30 mM, 10 to 30 mM, or 20 mM.

38. 38. The pharmaceutical composition of any one of claims 35 to 37, having a sodium chloride concentration of 80 to 200 mM, 100 to 175 mM, 120 to 150 mM, or 140 mM.

39. 39. The pharmaceutical composition of any one of claims 35 to 38, having a polysorbate 80 concentration of 0.005 mg / mL to 0.5 mg / mL, 0.01 mg / mL to 0.1 mg / mL, or 0.05 mg / mL.

40. 40. The pharmaceutical composition of any one of claims 35 to 39, wherein the pharmaceutically acceptable carrier comprises at least one sugar.

41. 41. The pharmaceutical composition of claim 40, having a sugar concentration of 0.5% to 20%, 1% to 10%, 1% to 5%, or 1% to 3%.

42. 42. The pharmaceutical composition of any one of claims 35 to 41, wherein the pharmaceutically acceptable carrier comprises sucrose, trehalose, D-mannitol, maltose and / or sorbitol.

43. 43. The pharmaceutical composition of any one of claims 35 to 42, comprising an antibacterial agent.

44. 44. The pharmaceutical composition of any one of claims 35 to 43, comprising m-cresol or methylparaben.

45. 45. The pharmaceutical composition of any one of claims 35 to 44, comprising 0.2% m-cresol and / or 0.9% methylparaben.

46. 46. ​​The pharmaceutical composition of any one of claims 35 to 45, wherein the anti-IL31 antibody is an antibody of any one of claims 1 to 30.

47. A method for treating a companion animal species having an IL31-induced condition, comprising administering to the companion animal species a therapeutically effective amount of an antibody described in any one of claims 1 to 30 or a pharmaceutical composition described in any one of claims 34 to 46.

48. 48. The method of claim 47, wherein the companion animal species is a dog, cat or horse.

49. 49. The method of claim 47 or claim 48, wherein the IL31-induced condition is a pruritic or allergic condition.

50. 50. The method of any one of claims 47 to 49, wherein the IL31-induced condition is selected from atopic dermatitis, pruritus, asthma, psoriasis, scleroderma, and eczema.

51. 51. The method of any one of claims 47 to 50, wherein the antibody or pharmaceutical composition is administered parenterally.

52. 52. The method of any one of claims 47 to 51, wherein the antibody or pharmaceutical composition is administered by intramuscular, intraperitoneal, intracerebrospinal, subcutaneous, intra-arterial, intrasynovial, intrathecal or inhalation route.

53. 53. The method of any one of claims 47 to 52, comprising administering a Jak inhibitor, a PI3K inhibitor, an AKT inhibitor or a MAPK inhibitor in combination with the antibody or pharmaceutical composition.

54. 54. The method of any one of claims 47 to 53, comprising administering one or more antibodies selected from an anti-IL17 antibody, an anti-TNFα antibody, an anti-CD20 antibody, an anti-CD19 antibody, an anti-CD25 antibody, an anti-IL4 antibody, an anti-IL13 antibody, an anti-IL23 antibody, an anti-IgE antibody, an anti-CD11α antibody, an anti-IL6R antibody, an anti-α4-integrin antibody, an anti-IL12 antibody, an anti-IL1β antibody, and an anti-BlyS antibody in combination with the antibody or pharmaceutical composition.

55. A method for reducing IL31 signaling function in a cell, comprising exposing the cell to an antibody described in any one of claims 1 to 30 or a pharmaceutical composition described in any one of claims 34 to 46 under conditions that allow binding of the antibody to extracellular IL31, thereby reducing binding to the IL31 receptor and / or reducing IL31 signaling function by the cell.

56. 56. The method of claim 55, wherein the cells are exposed to the antibody or pharmaceutical composition ex vivo.

57. 56. The method of claim 55, wherein the cell is exposed to the antibody or pharmaceutical composition in vivo.

58. 58. The method of any one of claims 55 to 57, wherein the cell is a canine, feline or equine cell.

59. A method for detecting IL31 in a sample from a companion animal species, comprising contacting the sample with an antibody described in any one of claims 1 to 30 or a pharmaceutical composition described in any one of claims 34 to 46 under conditions that allow binding of the antibody to IL31, and detecting whether a complex is formed between the antibody and IL31 in the sample.

60. 60. The method of claim 59, wherein the sample is a biological sample obtained from a dog, cat, or horse.

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