Esophageal cancer detection kit or device, and detection method

Abstract

To provide a kit or a device for the detection of esophageal cancer and a method for detecting esophageal cancer.SOLUTION: The present invention provides a kit or a device for the detection of esophageal cancer, comprising nucleic acid(s) capable of specifically binding to miRNA(s) in a sample of a subject, and a method for detecting esophageal cancer, comprising measuring the miRNA in vitro.SELECTED DRAWING: None

Description

[Technical Field]

[0001] The present invention relates to a specific method for detecting esophageal cancer in a subject. A kit or device for detecting esophageal cancer, comprising a nucleic acid capable of specifically binding to miRNA; and A method for detecting esophageal cancer, which comprises measuring the expression level of the miRNA using the nucleic acid. do. [Background technology]

[0002] The esophagus is a tube-like organ that carries food from the mouth to the stomach and is located between the trachea and the spine. It is divided into four layers from the inside to the outside: the mucosa, submucosa, muscularis propria, and adventitia. and the function of transporting food from the mouth to the stomach (Non-Patent Document 1). The National Cancer Center's Cancer Control Information Center discloses the number of cases in Japan in 2012. According to cancer statistics by region, the number of deaths from esophageal cancer was 11,592, and by location In Japan, the risk of death from esophageal cancer is Men are 5.6 times more likely to develop esophageal cancer than women, and smoking and alcohol consumption are known to be risk factors for esophageal cancer. In the United States, the incidence is 1 in 125 men and 435 in women (Non-Patent Document 1). It is said that one in five people will develop esophageal cancer, and the estimated number of people affected by esophageal cancer in 2014 was The number of cases is estimated to be as high as 18,170, of which approximately 15,450 will die (Non-patent Document 1 ).

[0003] The progression of esophageal cancer is defined in Non-Patent Document 2 and is based on the size of the tumor (Tis, T1-3). Stages are classified according to the stage of the disease, such as T4a-b, lymph node metastasis (N1-3), and distant metastasis (M0-1). Stage 0 (Tis / N0 / M0), Stage IA (T1 / N0 / M0), Stage IB (T 2 / N0 / M0), Stage IIA (T3 / N0 / M0), Stage IIB (T1-2 / N1 / M0), stage IIIA (T4a / N0 / M0, T3 / N1 / M0, and T1 ~2 / N2 / M0), Stage IIIB (T3 / N2 / M0), Stage IIIC (T4 a / N1-2 / M0, T4b / M0, and N3 / M0), and stage IV (M1) It is classified as follows.

[0004] The 5-year relative survival rate for esophageal cancer depends largely on the stage of the cancer. 39% for cancers that are confined to adjacent tissues, 21% for cancers that have metastasized to distant tissues, and 4% for cancers that have spread to distant tissues. It has been reported (Non-patent Document 1). Therefore, early detection of esophageal cancer is important for improving survival rates. There is a strong need for a method that enables early detection, as this would lead to a significant improvement in the quality of life.

[0005] Treatment for esophageal cancer is determined based on the stage of the cancer and the patient's overall condition, and mainly involves endoscopic treatment, surgery, There are radiation therapy and anti-cancer drugs. For cancers that have progressed to a certain extent, these treatments are combined. Multidisciplinary treatment is carried out to take advantage of the characteristics of each modality and create a synergistic effect (Non-patent Document 1) In addition, for early stage esophageal cancer, such as stage 0 or 1, endoscopic treatment, which places less strain on the patient, is available. In some cases, photodynamic therapy or phototherapeutic agent may be applicable (Non-Patent Document 1).

[0006] According to Non-Patent Document 1, the common tests for esophageal cancer are esophagography using X-rays and endoscopic examination. In addition, CT scans, MRI scans, and endoscopic ultrasound scans are also used to see the extent of cancer spread. If these initial tests reveal findings that suggest esophageal cancer, As a secondary test, a pathological examination is performed in which a needle is inserted into the lesion to collect cells or tissue and examine them under a microscope. In addition, blood tumor markers for detecting esophageal cancer include CEA and SCC. is known (Non-Patent Document 3).

[0007] Although it is still in the research stage, as shown in Patent Document 1, it is possible to use biological samples such as blood. Expression levels of microRNAs (miRNAs) in the brain, or the relationship between miRNA expression levels and other proteins It has been reported that esophageal cancer can be detected by combining the expression level of markers. .

[0008] Patent Document 1 describes the effects of miR-663a, miR-92a-3p, and miR-575 in serum. A method for detecting esophageal cancer by measuring miRNAs such as these has been demonstrated. [Prior art documents] [Patent documents]

[0009] [Patent Document 1] US Patent Application Publication No. 2014 / 031246 [Non-patent literature]

[0010] [Non-Patent Document 1] Edited by American Cancer Society, "Esophagus Cancer," 2014, pp. 2-8, 19-20, 29-41 [Non-patent document 2] Sobin, L. et al., “TNM Classification of Malignant Tumors 7th Edition Japanese Version” 2009, p.63-68 [Non-patent document 3] Terada, T. et al., 2013, International Journal of Clinical and Experimental Medicine, Vol. 6(3), pp. 219-26 Summary of the Invention [Problem to be solved by the invention]

[0011] The object of the present invention is to discover a novel esophageal cancer tumor marker and to develop a compound that specifically binds to the marker. The object of the present invention is to provide a method for effectively detecting esophageal cancer using nucleic acids.

[0012] As described in Non-Patent Document 1, the general examination for esophageal cancer is esophagography using X-rays. However, regular checkups focus on stomach cancer testing, and The roads are often not adequately observed. However, the number of deaths from esophageal cancer in Japan is steadily increasing, and imaging diagnosis is not necessarily the best treatment for esophageal cancer. It cannot be said that cancer is being suppressed. Also, CT scans and MRI scans are highly sophisticated and can only detect esophageal cancer. Although it is possible to detect the Therefore, it is not suitable for widespread use as a primary test.

[0013] Blood tumor markers for detecting esophageal cancer include CEA and SCC. However, these markers are also elevated in cancers other than esophageal cancer (Non-Patent Document 3), and Due to the toxicity issues, their usefulness has not been established. Therefore, if other cancers are misdiagnosed as esophageal cancer, opportunities for appropriate treatment may be missed or the wrong medical treatment may be given. This would impose unnecessary financial and physical burdens on patients. The American Cancer Society's Esophageal Cancer Guide also does not mention these markers (non-patent literature). 1).

[0014] Although still in the research stage, microRNAs (miR) in biological samples such as blood There are reports on the use of the expression level of NA to distinguish esophageal cancer, as shown below. It has not yet been put into practical use.

[0015] Patent Document 1 describes the effects of miR-663a, miR-92a-3p, and miR-575 in serum. A method for detecting esophageal cancer by measuring miRNAs such as A list of miRNAs that were altered in the serum of 16 esophageal cancer patients compared with 12 patients was shown. The expression level of the miRNA is measured to determine whether or not the patient has esophageal cancer. The method is an example of the detection performance, such as the specific accuracy, sensitivity, and specificity, of identifying esophageal cancer. The only verified hsa-miR- 345 has an AUC of 0.814, and it has been reported that it is difficult to distinguish esophageal cancer using it alone.

[0016] Thus, existing tumor markers have poor performance in detecting esophageal cancer, or The performance and detection methods of research-stage markers have not been specifically described, so these When using this method, unnecessary additional testing can be avoided by misidentifying healthy subjects as esophageal cancer patients. There is a possibility that treatment opportunities may be missed due to overlooking the procedure or overlooking esophageal cancer patients. Measuring miRNAs consisting of tens to hundreds of miRNAs increases the cost of testing, so It is difficult to use for large-scale screening such as breast cancer screening. Therefore, collecting esophageal tissue is highly invasive and undesirable for the patient, so minimally invasive methods are being considered. It is possible to detect it from blood samples that can be collected, and patients with esophageal cancer are considered to be esophageal cancer patients, and There is a need for a highly accurate esophageal cancer marker that can correctly distinguish between healthy and unhealthy subjects. In particular, early detection and treatment of esophageal cancer can significantly improve survival rates. In terms of treatment options, endoscopic therapy and photodynamic therapy, which are less invasive for patients, are now available. Therefore, there is a strong need for a highly sensitive esophageal cancer marker that can detect even low-stage esophageal cancer. It is being done. [Means for solving the problem]

[0017] As a result of intensive research aimed at solving the above problems, the present inventors have discovered a blood marker for detecting esophageal cancer. By identifying several genes that can be used for the purpose and using nucleic acids that can specifically bind to these genes, The present inventors have found that esophageal cancer can be significantly detected by this method, and have completed the present invention. <Summary of the Invention> That is, the present invention has the following features. (1) Esophageal cancer markers miR-204-3p, miR-1247-3p, and mi R-6875-5p, miR-6857-5p, miR-6726-5p, miR-31 88, miR-8069, miR-4257, miR-1343-3p, miR-710 8-5p, miR-6825-5p, miR-7641, miR-3185, miR-4 746-3p, miR-6791-5p, miR-6893-5p, miR-4433b -3p, miR-3135b, miR-6781-5p, miR-1908-5p, mi R-4792, miR-7845-5p, miR-4417, miR-3184-5p, miR-1225-5p, miR-1231, miR-1225-3p, miR-150 -3p、miR-4433-3p、miR-6125、miR-4513、miR-67 87-5p、miR-6784-5p、miR-615-5p、miR-6765-3p miR-5572, miR-6842-5p, miR-8063, miR-6780b -5p、miR-187-5p、miR-128-1-5p、miR-6729-5p、 miR-6741-5p、miR-6757-5p、miR-7110-5p、miR- 7975、miR-1233-5p、miR-6845-5p、miR-3937、mi R-4467、miR-7109-5p、miR-6088、miR-6782-5p、 miR-5195-3p, miR-4454, miR-6724-5p, miR-807 2, miR-4516, miR-6756-5p, miR-4665-3p, miR-6 826-5p、miR-6820-5p、miR-6887-5p、miR-3679- 5p、miR-7847-3p、miR-6721-5p、miR-3622a-5p、 miR-939-5p、miR-602、miR-7977、miR-6749-5p、 miR-1914-3p, miR-4651, miR-4695-5p, miR-684 8-5p、miR-1228-3p、miR-642b-3p、miR-6746-5p miR-3620-5p, miR-3131, miR-6732-5p, miR-71 13-3p、miR-23a-3p、miR-3154、miR-4723-5p、mi R-3663-3p、miR-4734、miR-6816-5p、miR-4442、 miR-4476, miR-423-5p, miR-1249, miR-6515-3p miR-887-3p, miR-4741, miR-6766-3p, miR-467 3, miR-6779-5p, miR-4706, miR-1268b, miR-463 2-5p、miR-3197、miR-6798-5p、miR-711、miR-68 40-3p、miR-6763-5p、miR-6727-5p、miR-371a-5 p、miR-6824-5p、miR-4648、miR-1227-5p、miR-5 64、miR-3679-3p、miR-2861、miR-6737-5p、miR- 4725-3p、miR-6716-5p、miR-4675、miR-1915-3p miR-671-5p, miR-3656, miR-6722-3p, miR-470 7-5p、miR-4449、miR-1202、miR-4649-5p、miR-7 44-5p、miR-642a-3p、miR-451a、miR-6870-5p、m iR-4443、miR-6808-5p、miR-4728-5p、miR-937- 5p、miR-135a-3p、miR-663b、miR-1343-5p、miR- 6822-5p、miR-6803-5p、miR-6805-3p、miR-128- 2-5p、miR-4640-5p、miR-1469、miR-92a-2-5p、m iR-3940-5p、miR-4281、miR-1260b、miR-4758-5 p、miR-1915-5p、miR-5001-5p、miR-4286、miR-6 126, miR-6789-5p, miR-4459, miR-1268a, miR-6 752-5p、miR-6131、miR-6800-5p、miR-4532、miR -6872-3p, miR-718, miR-6769a-5p, miR-4707-3 p, miR-6765-5p, miR-4739, miR-4525, miR-4270 , miR-4534, miR-6785-5p, miR-6850-5p, miR-46 97-5p, miR-1260a, miR-4486, miR-6880-5p, miR -6802-5p, miR-6861-5p, miR-92b-5p, miR-1238 -5p, miR-6851-5p, miR-7704, miR-149-3p, miR- 4689, miR-4688, miR-125a-3p, miR-23b-3p, miR -614, miR-1913, miR-16-5p, miR-6717-5p, miR- 3648, miR-3162-5p, miR-1909-3p, miR-8073, mi R-6769b-5p, miR-6836-3p, miR-4484, miR-6819 at least one polynucleotide selected from the group consisting of miR-5p and miR-6794-5p A kit for detecting esophageal cancer, comprising a nucleic acid capable of specifically binding to a protease inhibitor. (2) miR-204-3p is hsa-miR-204-3p and miR-1247 -3p is hsa-miR-1247-3p, and miR-6875-5p is hsa-m iR-6875-5p and miR-6857-5p is hsa-miR-6857-5 p, miR-6726-5p is hsa-miR-6726-5p, and miR- 3188 is hsa-miR-3188 and miR-8069 is hsa-miR-80 69, miR-4257 is hsa-miR-4257, and miR-1343- 3p is hsa-miR-1343-3p, and miR-7108-5p is hsa-mi R-7108-5p and miR-6825-5p are hsa-miR-6825-5p , miR-7641 is hsa-miR-7641, and miR-3185 is hs a-miR-3185 and miR-4746-3p is hsa-miR-4746-3 p, miR-6791-5p is hsa-miR-6791-5p, and miR- 6893-5p is hsa-miR-6893-5p, and miR-4433b-3p is hsa-miR-4433b-3p, and miR-3135b is hsa-miR-31 35b, miR-6781-5p is hsa-miR-6781-5p, and mi R-1908-5p is hsa-miR-1908-5p, and miR-4792 is hs a-miR-4792 and miR-7845-5p is hsa-miR-7845-5 p, miR-4417 is hsa-miR-4417, and miR-3184-5 p is hsa-miR-3184-5p and miR-1225-5p is hsa-miR -1225-5p, miR-1231 is hsa-miR-1231, and miR -1225-3p is hsa-miR-1225-3p, and miR-150-3p is h sa-miR-150-3p and miR-4433-3p are hsa-miR-443 3-3p, miR-6125 is hsa-miR-6125, and miR-451 3 is hsa-miR-4513, and miR-6787-5p is hsa-miR-67 87-5p, and miR-6784-5p is hsa-miR-6784-5p; miR-615-5p is hsa-miR-615-5p, and miR-6765-3p is hsa-miR-6765-3p, and miR-5572 is hsa-miR-557 2, miR-6842-5p is hsa-miR-6842-5p, and miR- 8063 is hsa-miR-8063, and miR-6780b-5p is hsa-mi miR-6780b-5p and miR-187-5p are hsa-miR-187-5p. miR-128-1-5p is hsa-miR-128-1-5p, and miR- 6729-5p is hsa-miR-6729-5p and miR-6741-5p is h sa-miR-6741-5p and miR-6757-5p are hsa-miR-67 57-5p, miR-7110-5p is hsa-miR-7110-5p, miR-7975 is hsa-miR-7975, and miR-1233-5p is hsa -miR-1233-5p and miR-6845-5p are hsa-miR-6845 -5p, miR-3937 is hsa-miR-3937, and miR-4467 is hsa-miR-4467 and miR-7109-5p is hsa-miR-710 9-5p, miR-6088 is hsa-miR-6088, and miR-678 2-5p is hsa-miR-6782-5p, and miR-5195-3p is hsa- miR-5195-3p, miR-4454 is hsa-miR-4454, miR-6724-5p is hsa-miR-6724-5p, and miR-8072 is hsa-miR-8072 and miR-4516 , miR-6756-5p is hsa-miR-6756-5p, and miR-4665 -3p is hsa-miR-4665-3p, and miR-6826-5p is hsa-m iR-6826-5p and miR-6820-5p are hsa-miR-6820-5 p, miR-6887-5p is hsa-miR-6887-5p, and miR- 3679-5p is hsa-miR-3679-5p, and miR-7847-3p is h sa-miR-7847-3p and miR-6721-5p are hsa-miR-67 21-5p, and miR-3622a-5p is hsa-miR-3622a-5p. miR-939-5p is hsa-miR-939-5p, and miR-602 is hsa-miR-939-5p. sa-miR-602, miR-7977 is hsa-miR-7977, m iR-6749-5p is hsa-miR-6749-5p, and miR-1914-3 p is hsa-miR-1914-3p, and miR-4651 is hsa-miR-46 51, miR-4695-5p is hsa-miR-4695-5p, and miR -6848-5p is hsa-miR-6848-5p, and miR-1228-3p is hsa-miR-1228-3p and miR-642b-3p are hsa-miR-6 42b-3p, miR-6746-5p is hsa-miR-6746-5p , miR-3620-5p is hsa-miR-3620-5p, and miR-3131 is hsa-miR-3131, and miR-6732-5p is hsa-miR-673 2-5p, miR-7113-3p is hsa-miR-7113-3p, and m iR-23a-3p is hsa-miR-23a-3p, and miR-3154 is hsa -miR-3154 and miR-4723-5p is hsa-miR-4723-5p , miR-3663-3p is hsa-miR-3663-3p, and miR-4 734 is hsa-miR-4734, and miR-6816-5p is hsa-miR- 6816-5p, miR-4442 is hsa-miR-4442, and miR- 4476 is hsa-miR-4476, and miR-423-5p is hsa-miR- 423-5p, miR-1249 is hsa-miR-1249, and miR-6 515-3p is hsa-miR-6515-3p, and miR-887-3p is hsa -miR-887-3p, and miR-4741 is hsa-miR-4741; miR-6766-3p is hsa-miR-6766-3p, and miR-4673 is hsa-miR-4673 and miR-6779-5p are hsa-miR-6779 -5p, miR-4706 is hsa-miR-4706, and miR-1268 b is hsa-miR-1268b, and miR-4632-5p is hsa-miR-4 632-5p, miR-3197 is hsa-miR-3197, and miR-6 798-5p is hsa-miR-6798-5p, and miR-711 is hsa-mi R-711, and miR-6840-3p is hsa-miR-6840-3p; miR-6763-5p is hsa-miR-6763-5p, and miR-6727- 5p is hsa-miR-6727-5p, and miR-371a-5p is hsa-mi miR-371a-5p and miR-6824-5p are hsa-miR-6824-5p , miR-4648 is hsa-miR-4648, and miR-1227-5p is hsa-miR-1227-5p and miR-564 is hsa-miR-564 miR-3679-3p is hsa-miR-3679-3p, and miR-28 61 is hsa-miR-2861, and miR-6737-5p is hsa-miR-6 737-5p, and miR-4725-3p is hsa-miR-4725-3p , miR-6716-5p is hsa-miR-6716-5p, and miR-4675 is hsa-miR-4675 and miR-1915-3p is hsa-miR-191 5-3p, miR-671-5p is hsa-miR-671-5p, and miR -3656 is hsa-miR-3656, and miR-6722-3p is hsa-mi miR-6722-3p and miR-4707-5p are hsa-miR-4707-5p , miR-4449 is hsa-miR-4449, and miR-1202 is hs a-miR-1202 and miR-4649-5p is hsa-miR-4649-5 p, miR-744-5p is hsa-miR-744-5p, and miR-64 2a-3p is hsa-miR-642a-3p, and miR-451a is hsa-mi R-451a, miR-6870-5p is hsa-miR-6870-5p , miR-4443 is hsa-miR-4443, and miR-6808-5p is hs a-miR-6808-5p and miR-4728-5p are hsa-miR-472 8-5p, miR-937-5p is hsa-miR-937-5p, and miR -135a-3p is hsa-miR-135a-3p, and miR-663b is hsa -miR-663b and miR-1343-5p is hsa-miR-1343-5p , miR-6822-5p is hsa-miR-6822-5p, and miR-6 803-5p is hsa-miR-6803-5p, and miR-6805-3p is hs a-miR-6805-3p and miR-128-2-5p are hsa-miR-12 8-2-5p, miR-4640-5p is hsa-miR-4640-5p , miR-1469 is hsa-miR-1469, and miR-92a-2-5p is h sa-miR-92a-2-5p and miR-3940-5p are hsa-miR-3 940-5p, miR-4281 is hsa-miR-4281, and miR-1 260b is hsa-miR-1260b, and miR-4758-5p is hsa-mi R-4758-5p and miR-1915-5p are hsa-miR-1915-5p , miR-5001-5p is hsa-miR-5001-5p, and miR-4 286 is hsa-miR-4286 and miR-6126 is hsa-miR-612 6, miR-6789-5p is hsa-miR-6789-5p, and miR- 4459 is hsa-miR-4459, and miR-1268a is hsa-miR-1 268a, miR-6752-5p is hsa-miR-6752-5p, and m iR-6131 is hsa-miR-6131, and miR-6800-5p is hsa- miR-6800-5p, miR-4532 is hsa-miR-4532, miR-6872-3p is hsa-miR-6872-3p, and miR-718 is h sa-miR-718 and miR-6769a-5p are hsa-miR-6769a -5p, miR-4707-3p is hsa-miR-4707-3p, and mi R-6765-5p is hsa-miR-6765-5p, and miR-4739 is hs a-miR-4739, miR-4525 is hsa-miR-4525, and m iR-4270 is hsa-miR-4270 and miR-4534 is hsa-miR -4534, and miR-6785-5p is hsa-miR-6785-5p; miR-6850-5p is hsa-miR-6850-5p, and miR-4697- 5p is hsa-miR-4697-5p, and miR-1260a is hsa-miR- 1260a, miR-4486 is hsa-miR-4486, and miR-68 80-5p is hsa-miR-6880-5p, and miR-6802-5p is hsa -miR-6802-5p, and miR-6861-5p is hsa-miR-6861 -5p, miR-92b-5p is hsa-miR-92b-5p, and miR- 1238-5p is hsa-miR-1238-5p, and miR-6851-5p is h sa-miR-6851-5p and miR-7704 is hsa-miR-7704 miR-149-3p is hsa-miR-149-3p, and miR-4689 is hsa-miR-4689 and miR-4688 is hsa-miR-4688 miR-125a-3p is hsa-miR-125a-3p, and miR-23b is -3p is hsa-miR-23b-3p, and miR-614 is hsa-miR-61 4, miR-1913 is hsa-miR-1913, and miR-16-5p is hsa-miR-16-5p and miR-6717-5p are hsa-miR-671 7-5p, miR-3648 is hsa-miR-3648, and miR-316 2-5p is hsa-miR-3162-5p, and miR-1909-3p is hsa- miR-1909-3p, miR-8073 is hsa-miR-8073, miR-6769b-5p is hsa-miR-6769b-5p, and miR-683 6-3p is hsa-miR-6836-3p, and miR-4484 is hsa-miR -4484, and miR-6819-5p is hsa-miR-6819-5p; and miR-6794-5p is hsa-miR-6794-5p, as described in (1). Kit. (3) The nucleic acid is a polynucleotide shown in (a) to (e) below: (a) a sequence number represented by any one of SEQ ID NOs: 1 to 115, 117 to 189, and 666 to 675; or a polynucleotide consisting of a base sequence in which u is t a fragment thereof containing 15 or more consecutive bases, (b) SEQ ID NOs: 1 to 115, 117 to 189, and 666 to 675 a polynucleotide comprising a base sequence (c) SEQ ID NOs: 1 to 115, 117 to 189, and 666 to 675 or a base sequence complementary to the base sequence in which u is t a polynucleotide comprising 15 or more consecutive bases, a variant thereof, a derivative thereof, or a polynucleotide comprising 15 or more consecutive bases Fragments of, (d) SEQ ID NOs: 1 to 115, 117 to 189, and 666 to 675 or a base sequence complementary to the base sequence in which u is t in the base sequence. a polynucleotide comprising the (e) A polynucleotide that binds to any one of the polynucleotides (a) to (d) under stringent conditions. hybridizing polynucleotides, The kit according to (1) or (2), wherein the polynucleotide is selected from the group consisting of: (4) The kit detects miR-575 and miR-24-3, which are other esophageal cancer markers. (1) to (3) further comprising a nucleic acid capable of specifically binding to a polynucleotide consisting of p The kit according to any one of the above. (5) miR-575 is hsa-miR-575 and miR-24-3p is hsa- The kit according to (4), wherein the miR-24-3p is used. (6) The nucleic acid is a polynucleotide shown in (f) to (j) below: (f) the base sequence represented by SEQ ID NOs: 116 and 676 or the base sequence in which u is a polynucleotide consisting of a base sequence of t, a variant thereof, a derivative thereof, or 15 or more a fragment thereof containing consecutive bases, (g) a polynucleotide comprising the base sequences represented by SEQ ID NOs: 116 and 676; (h) the base sequence represented by SEQ ID NOs: 116 and 676 or the base sequence in which u is Polynucleotides consisting of a base sequence complementary to the base sequence t, their variants, and their derivatives a fragment thereof containing 15 or more consecutive bases, (i) the base sequence represented by SEQ ID NOs: 116 and 676 or the base sequence in which u is a polynucleotide comprising a base sequence complementary to the base sequence of t, and (j) A method for detecting a polynucleotide that is hybridized with any one of the polynucleotides (f) to (i) under stringent conditions. hybridizing polynucleotides, The kit according to (4) or (5), wherein the polynucleotide is selected from the group consisting of: (7) The kit further comprises a marker for esophageal cancer, such as miR-675-5p or miR-4. 86-3p, miR-6777-5p, miR-4497, miR-296-3p, mi R-6738-5p, miR-4731-5p, miR-6889-5p, miR-67 86-5p, miR-92a-3p, miR-4294, miR-4763-3p, mi R-6076, miR-663a, miR-760, miR-4667-5p, miR- 6090, miR-4730, miR-7106-5p, miR-3196, miR-5 698, miR-6087, miR-4665-5p, miR-8059 and miR-6 879-5p, which specifically binds to at least one polynucleotide selected from the group consisting of The kit according to any one of (1) to (6), further comprising a nucleic acid capable of synthesizing. (8) miR-675-5p is hsa-miR-675-5p, and miR-486- 3p is hsa-miR-486-3p, and miR-6777-5p is hsa-miR -6777-5p, miR-4497 is hsa-miR-4497, and miR -296-3p is hsa-miR-296-3p, and miR-6738-5p is hs a-miR-6738-5p and miR-4731-5p are hsa-miR-473 1-5p, miR-6889-5p is hsa-miR-6889-5p, and m iR-6786-5p is hsa-miR-6786-5p, and miR-92a-3p is hsa-miR-92a-3p and miR-4294 is hsa-miR-4294 , miR-4763-3p is hsa-miR-4763-3p, and miR-6 076 is hsa-miR-6076, and miR-663a is hsa-miR-663 a, miR-760 is hsa-miR-760, and miR-4667-5p is hsa-miR-4667-5p and miR-6090 , miR-4730 is hsa-miR-4730, and miR-7106-5p is hsa-miR-7106-5p, and miR-3196 is hsa-miR-319 6, miR-5698 is hsa-miR-5698, and miR-6087 is h sa-miR-6087 and miR-4665-5p is hsa-miR-4665- 5p, miR-8059 is hsa-miR-8059, and miR-68 The kit according to (7), wherein 79-5p is hsa-miR-6879-5p. (9) The nucleic acid is a polynucleotide represented by any one of (k) to (o) below: (k) a base sequence represented by any one of SEQ ID NOs: 190 to 214 or the base sequence a polynucleotide consisting of a base sequence in which u is t, a variant thereof, a derivative thereof, or a fragment thereof containing 5 or more consecutive bases; (l) a polynucleotide comprising a nucleotide sequence represented by any one of SEQ ID NOs: 190 to 214; (m) a base sequence represented by any one of SEQ ID NOs: 190 to 214 or the base sequence a polynucleotide consisting of a base sequence complementary to the base sequence in which u is t, a variant thereof, its derivatives or fragments containing 15 or more consecutive bases; (n) a base sequence represented by any one of SEQ ID NOs: 190 to 214 or the base sequence a polynucleotide comprising a base sequence complementary to the base sequence in which u is t; (o) a polynucleotide that binds to any one of the polynucleotides (k) to (n) under stringent conditions; hybridizing polynucleotides, The kit according to (7) or (8), wherein the polynucleotide is selected from the group consisting of: (10) The kit includes a marker selected from all of the esophageal cancer markers described in (1) or (2). At least two polynucleotides capable of specifically binding to each of the at least two polynucleotides The kit according to any one of (1) to (9), comprising the nucleic acid: (11) Esophageal cancer markers miR-204-3p, miR-1247-3p, and m iR-6875-5p, miR-6857-5p, miR-6726-5p, miR-3 188, miR-8069, miR-4257, miR-1343-3p, miR-71 08-5p, miR-6825-5p, miR-7641, miR-3185, miR- 4746-3p, miR-6791-5p, miR-6893-5p, miR-4433 b-3p, miR-3135b, miR-6781-5p, miR-1908-5p, m iR-4792, miR-7845-5p, miR-4417, miR-3184-5p , miR-1225-5p, miR-1231, miR-1225-3p, miR-15 0-3p, miR-4433-3p, miR-6125, miR-4513, miR-6 787-5p, miR-6784-5p, miR-615-5p, miR-6765-3 p, miR-5572, miR-6842-5p, miR-8063, miR-6780 b-5p, miR-187-5p, miR-128-1-5p, miR-6729-5p , miR-6741-5p, miR-6757-5p, miR-7110-5p, miR -7975, miR-1233-5p, miR-6845-5p, miR-3937, m iR-4467, miR-7109-5p, miR-6088, miR-6782-5p , miR-5195-3p, miR-4454, miR-6724-5p, miR-80 72, miR-4516, miR-6756-5p, miR-4665-3p, miR- 6826-5p、miR-6820-5p、miR-6887-5p、miR-3679 -5p、miR-7847-3p、miR-6721-5p、miR-3622a-5p miR-939-5p, miR-602, miR-7977, miR-6749-5p miR-1914-3p, miR-4651, miR-4695-5p, miR-68 48-5p、miR-1228-3p、miR-642b-3p、miR-6746-5 p、miR-3620-5p、miR-3131、miR-6732-5p、miR-7 113-3p、miR-23a-3p、miR-3154、miR-4723-5p、m iR-3663-3p、miR-4734、miR-6816-5p、miR-4442 miR-4476, miR-423-5p, miR-1249, miR-6515-3 p、miR-887-3p、miR-4741、miR-6766-3p、miR-46 73, miR-6779-5p, miR-4706, miR-1268b, miR-46 32-5p、miR-3197、miR-6798-5p、miR-711、miR-6 840-3p、miR-6763-5p、miR-6727-5p、miR-371a- 5p、miR-6824-5p、miR-4648、miR-1227-5p、miR- 564、miR-3679-3p、miR-2861、miR-6737-5p、miR -4725-3p、miR-6716-5p、miR-4675、miR-1915-3 p、miR-671-5p、miR-3656、miR-6722-3p、miR-47 07-5p、miR-4449、miR-1202、miR-4649-5p、miR- 744-5p、miR-642a-3p、miR-451a、miR-6870-5p、 miR-4443, miR-6808-5p, miR-4728-5p, miR-937 -5p、miR-135a-3p、miR-663b、miR-1343-5p、miR -6822-5p、miR-6803-5p、miR-6805-3p、miR-128 -2-5p、miR-4640-5p、miR-1469、miR-92a-2-5p、 miR-3940-5p、miR-4281、miR-1260b、miR-4758- 5p、miR-1915-5p、miR-5001-5p、miR-4286、miR- 6126、miR-6789-5p、miR-4459、miR-1268a、miR- 6752-5p、miR-6131、miR-6800-5p、miR-4532、mi R-6872-3p、miR-718、miR-6769a-5p、miR-4707- 3p、miR-6765-5p、miR-4739、miR-4525、miR-427 0, miR-4534, miR-6785-5p, miR-6850-5p, miR-4 697-5p、miR-1260a、miR-4486、miR-6880-5p、mi R-6802-5p、miR-6861-5p、miR-92b-5p、miR-123 8-5p、miR-6851-5p、miR-7704、miR-149-3p、miR -4689、miR-4688、miR-125a-3p、miR-23b-3p、mi R-614、miR-1913、miR-16-5p、miR-6717-5p、miR -3648、miR-3162-5p、miR-1909-3p、miR-8073、m iR-6769b-5p、miR-6836-3p、miR-4484、miR-681 at least one polynucleotide selected from the group consisting of miR-9-5p and miR-6794-5p A device for detecting esophageal cancer, comprising a nucleic acid capable of specifically binding to a nucleotide. (12) miR-204-3p is hsa-miR-204-3p, and miR-124 7-3p is hsa-miR-1247-3p, and miR-6875-5p is hsa- miR-6875-5p and miR-6857-5p are hsa-miR-6857- 5p, miR-6726-5p is hsa-miR-6726-5p, and miR -3188 is hsa-miR-3188, and miR-8069 is hsa-miR-8 069, miR-4257 is hsa-miR-4257, and miR-1343 -3p is hsa-miR-1343-3p, and miR-7108-5p is hsa-m iR-7108-5p and miR-6825-5p are hsa-miR-6825-5 p, miR-7641 is hsa-miR-7641, and miR-3185 is h sa-miR-3185 and miR-4746-3p is hsa-miR-4746- 3p, miR-6791-5p is hsa-miR-6791-5p, and miR -6893-5p is hsa-miR-6893-5p, and miR-4433b-3p is hsa-miR-4433b-3p, and miR-3135b is hsa-miR-3 135b, miR-6781-5p is hsa-miR-6781-5p, and m iR-1908-5p is hsa-miR-1908-5p, and miR-4792 is h sa-miR-4792 and miR-7845-5p is hsa-miR-7845- 5p, miR-4417 is hsa-miR-4417, and miR-3184- 5p is hsa-miR-3184-5p, and miR-1225-5p is hsa-mi miR-1225-5p, miR-1231 is hsa-miR-1231, and mi R-1225-3p is hsa-miR-1225-3p, and miR-150-3p is hsa-miR-150-3p and miR-4433-3p are hsa-miR-44 33-3p, miR-6125 is hsa-miR-6125, and miR-45 13 is hsa-miR-4513, and miR-6787-5p is hsa-miR-6 787-5p, miR-6784-5p is hsa-miR-6784-5p , miR-615-5p is hsa-miR-615-5p, and miR-6765-3 p is hsa-miR-6765-3p, and miR-5572 is hsa-miR-55 72, miR-6842-5p is hsa-miR-6842-5p, and miR -8063 is hsa-miR-8063, and miR-6780b-5p is hsa-m iR-6780b-5p and miR-187-5p is hsa-miR-187-5p and miR-128-1-5p is hsa-miR-128-1-5p, and miR -6729-5p is hsa-miR-6729-5p, and miR-6741-5p is hsa-miR-6741-5p and miR-6757-5p are hsa-miR-6 757-5p, miR-7110-5p is hsa-miR-7110-5p , miR-7975 is hsa-miR-7975, and miR-1233-5p is hs a-miR-1233-5p and miR-6845-5p are hsa-miR-684 5-5p, miR-3937 is hsa-miR-3937, and miR-446 7 is hsa-miR-4467, and miR-7109-5p is hsa-miR-71 09-5p, miR-6088 is hsa-miR-6088, and miR-67 82-5p is hsa-miR-6782-5p, and miR-5195-3p is hsa -miR-5195-3p, and miR-4454 is hsa-miR-4454 , miR-6724-5p is hsa-miR-6724-5p, and miR-8072 is hsa-miR-8072 and miR-4516 is hsa-miR-4516 miR-6756-5p is hsa-miR-6756-5p, and miR-466 5-3p is hsa-miR-4665-3p, and miR-6826-5p is hsa- miR-6826-5p and miR-6820-5p are hsa-miR-6820- 5p, miR-6887-5p is hsa-miR-6887-5p, and miR -3679-5p is hsa-miR-3679-5p, and miR-7847-3p is hsa-miR-7847-3p and miR-6721-5p are hsa-miR-6 721-5p, and miR-3622a-5p is hsa-miR-3622a-5p. miR-939-5p is hsa-miR-939-5p, and miR-602 is hsa-miR-602 and miR-7977 are hsa-miR-7977; miR-6749-5p is hsa-miR-6749-5p, and miR-1914- 3p is hsa-miR-1914-3p, and miR-4651 is hsa-miR-4 651, miR-4695-5p is hsa-miR-4695-5p, and mi R-6848-5p is hsa-miR-6848-5p, and miR-1228-3p is hsa-miR-1228-3p, and miR-642b-3p is hsa-miR- 642b-3p, and miR-6746-5p is hsa-miR-6746-5p. miR-3620-5p is hsa-miR-3620-5p, and miR-313 1 is hsa-miR-3131, and miR-6732-5p is hsa-miR-67 32-5p, and miR-7113-3p is hsa-miR-7113-3p; miR-23a-3p is hsa-miR-23a-3p, and miR-3154 is hs a-miR-3154 and miR-4723-5p is hsa-miR-4723-5 p, miR-3663-3p is hsa-miR-3663-3p, and miR- 4734 is hsa-miR-4734 and miR-6816-5p is hsa-miR -6816-5p, miR-4442 is hsa-miR-4442, and miR -4476 is hsa-miR-4476, and miR-423-5p is hsa-miR -423-5p, miR-1249 is hsa-miR-1249, and miR- 6515-3p is hsa-miR-6515-3p, and miR-887-3p is hs a-miR-887-3p, miR-4741 is hsa-miR-4741 , miR-6766-3p is hsa-miR-6766-3p, and miR-4673 is hsa-miR-4673 and miR-6779-5p is hsa-miR-677 9-5p, miR-4706 is hsa-miR-4706, and miR-126 8b is hsa-miR-1268b, and miR-4632-5p is hsa-miR- 4632-5p, miR-3197 is hsa-miR-3197, and miR- 6798-5p is hsa-miR-6798-5p, and miR-711 is hsa-m iR-711, miR-6840-3p is hsa-miR-6840-3p , miR-6763-5p is hsa-miR-6763-5p, and miR-6727 -5p is hsa-miR-6727-5p, and miR-371a-5p is hsa-m iR-371a-5p and miR-6824-5p are hsa-miR-6824-5 p, miR-4648 is hsa-miR-4648, and miR-1227-5 p is hsa-miR-1227-5p and miR-564 is hsa-miR-564 , miR-3679-3p is hsa-miR-3679-3p, and miR-2 861 is hsa-miR-2861, and miR-6737-5p is hsa-miR- 6737-5p, and miR-4725-3p is hsa-miR-4725-3p. miR-6716-5p is hsa-miR-6716-5p, and miR-467 5 is hsa-miR-4675, and miR-1915-3p is hsa-miR-19 15-3p, miR-671-5p is hsa-miR-671-5p, and mi R-3656 is hsa-miR-3656, and miR-6722-3p is hsa-m iR-6722-3p and miR-4707-5p are hsa-miR-4707-5 p, miR-4449 is hsa-miR-4449, and miR-1202 is h sa-miR-1202 and miR-4649-5p is hsa-miR-4649- 5p, miR-744-5p is hsa-miR-744-5p, and miR-6 42a-3p is hsa-miR-642a-3p, and miR-451a is hsa-m iR-451a and miR-6870-5p are hsa-miR-6870-5p. miR-4443 is hsa-miR-4443, and miR-6808-5p is hsa-miR-4443. sa-miR-6808-5p and miR-4728-5p are hsa-miR-47 28-5p, miR-937-5p is hsa-miR-937-5p, and mi R-135a-3p is hsa-miR-135a-3p, and miR-663b is hs a-miR-663b and miR-1343-5p is hsa-miR-1343-5 p, miR-6822-5p is hsa-miR-6822-5p, and miR- 6803-5p is hsa-miR-6803-5p and miR-6805-3p is h sa-miR-6805-3p and miR-128-2-5p are hsa-miR-1 28-2-5p, and miR-4640-5p is hsa-miR-4640-5p. miR-1469 is hsa-miR-1469, and miR-92a-2-5p is hsa-miR-92a-2-5p and miR-3940-5p are hsa-miR- 3940-5p, miR-4281 is hsa-miR-4281, and miR- 1260b is hsa-miR-1260b, and miR-4758-5p is hsa-m iR-4758-5p and miR-1915-5p are hsa-miR-1915-5 p, miR-5001-5p is hsa-miR-5001-5p, and miR- 4286 is hsa-miR-4286, and miR-6126 is hsa-miR-61 26, miR-6789-5p is hsa-miR-6789-5p, and miR -4459 is hsa-miR-4459, and miR-1268a is hsa-miR- 1268a, and miR-6752-5p is hsa-miR-6752-5p; miR-6131 is hsa-miR-6131, and miR-6800-5p is hsa -miR-6800-5p, and miR-4532 is hsa-miR-4532 , miR-6872-3p is hsa-miR-6872-3p, and miR-718 is hsa-miR-718 and miR-6769a-5p are hsa-miR-6769 a-5p, miR-4707-3p is hsa-miR-4707-3p, and m iR-6765-5p is hsa-miR-6765-5p, and miR-4739 is h sa-miR-4739, and miR-4525 is hsa-miR-4525; miR-4270 is hsa-miR-4270 and miR-4534 is hsa-mi R-4534, and miR-6785-5p is hsa-miR-6785-5p , miR-6850-5p is hsa-miR-6850-5p, and miR-4697 -5p is hsa-miR-4697-5p, and miR-1260a is hsa-miR -1260a, miR-4486 is hsa-miR-4486, and miR-6 880-5p is hsa-miR-6880-5p, and miR-6802-5p is hs a-miR-6802-5p and miR-6861-5p are hsa-miR-686 1-5p, miR-92b-5p is hsa-miR-92b-5p, and miR -1238-5p is hsa-miR-1238-5p, and miR-6851-5p is hsa-miR-6851-5p and miR-7704 , miR-149-3p is hsa-miR-149-3p, and miR-468 9 is hsa-miR-4689 and miR-4688 is hsa-miR-4688 miR-125a-3p is hsa-miR-125a-3p, and miR-23 b-3p is hsa-miR-23b-3p, and miR-614 is hsa-miR-6 14, miR-1913 is hsa-miR-1913, and miR-16-5p is hsa-miR-16-5p, and miR-6717-5p is hsa-miR-67 17-5p, miR-3648 is hsa-miR-3648, and miR-31 62-5p is hsa-miR-3162-5p, and miR-1909-3p is hsa -miR-1909-3p, and miR-8073 is hsa-miR-8073 , miR-6769b-5p is hsa-miR-6769b-5p, and miR-68 36-3p is hsa-miR-6836-3p, and miR-4484 is hsa-mi R-4484, and miR-6819-5p is hsa-miR-6819-5p , and miR-6794-5p is hsa-miR-6794-5p, (11) The device described. (13) The nucleic acid is a polynucleotide shown in any one of (a) to (e) below: (a) a sequence number represented by any one of SEQ ID NOs: 1 to 115, 117 to 189, and 666 to 675; or a polynucleotide consisting of a base sequence in which u is t a fragment thereof containing 15 or more consecutive bases, (b) SEQ ID NOs: 1 to 115, 117 to 189, and 666 to 675 a polynucleotide comprising a base sequence (c) SEQ ID NOs: 1 to 115, 117 to 189, and 666 to 675 or a base sequence complementary to the base sequence in which u is t a polynucleotide comprising 15 or more consecutive bases, a variant thereof, a derivative thereof, or a polynucleotide comprising 15 or more consecutive bases Fragments of, (d) SEQ ID NOs: 1 to 115, 117 to 189, and 666 to 675 or a base sequence complementary to the base sequence in which u is t in the base sequence. a polynucleotide comprising the (e) A polynucleotide that binds to any one of the polynucleotides (a) to (d) under stringent conditions. hybridizing polynucleotides, The device according to (11) or (12), which is a polynucleotide selected from the group consisting of Chair. (14) The device is capable of detecting miR-575 and miR-2, which are other esophageal cancer markers. (11) to (12) further comprising a nucleic acid capable of specifically binding to a polynucleotide consisting of 4-3p. (13) A device according to any one of (13). (15), miR-575 is hsa-miR-575, and miR-24-3p is hs The device according to (14), wherein the miR-24-3p is a-miR-24-3p. (16) The nucleic acid is a polynucleotide shown in (f) to (j) below: (f) the base sequence represented by SEQ ID NOs: 116 and 676 or the base sequence in which u is a polynucleotide consisting of a base sequence of t, a variant thereof, a derivative thereof, or 15 or more a fragment thereof containing consecutive bases, (g) a polynucleotide comprising the base sequences represented by SEQ ID NOs: 116 and 676; (h) the base sequence represented by SEQ ID NOs: 116 and 676 or the base sequence in which u is Polynucleotides consisting of a base sequence complementary to the base sequence t, their variants, and their derivatives a fragment thereof containing 15 or more consecutive bases, (i) the base sequence represented by SEQ ID NOs: 116 and 676 or the base sequence in which u is a polynucleotide comprising a base sequence complementary to the base sequence of t, and (j) A method for detecting a polynucleotide that is hybridized with any one of the polynucleotides (f) to (i) under stringent conditions. hybridizing polynucleotides, The device according to (14) or (15), which is a polynucleotide selected from the group consisting of Chair. (17) The device is capable of detecting another esophageal cancer marker, miR-675-5p, miR -486-3p, miR-6777-5p, miR-4497, miR-296-3p, miR-6738-5p, miR-4731-5p, miR-6889-5p, miR- 6786-5p, miR-92a-3p, miR-4294, miR-4763-3p, miR-6076, miR-663a, miR-760, miR-4667-5p, mi R-6090, miR-4730, miR-7106-5p, miR-3196, miR -5698, miR-6087, miR-4665-5p, miR-8059 and miR -6879-5p The device according to any one of (11) to (16), further comprising a nucleic acid capable of binding to (18) miR-675-5p is hsa-miR-675-5p, and miR-486 -3p is hsa-miR-486-3p, and miR-6777-5p is hsa-mi miR-6777-5p, miR-4497 is hsa-miR-4497, and mi R-296-3p is hsa-miR-296-3p, and miR-6738-5p is h sa-miR-6738-5p and miR-4731-5p are hsa-miR-47 31-5p, and miR-6889-5p is hsa-miR-6889-5p; miR-6786-5p is hsa-miR-6786-5p, and miR-92a-3 p is hsa-miR-92a-3p, and miR-4294 is hsa-miR-429 4, miR-4763-3p is hsa-miR-4763-3p, and miR- 6076 is hsa-miR-6076, and miR-663a is hsa-miR-66 3a, miR-760 is hsa-miR-760, and miR-4667-5p is hsa-miR-4667-5p, and miR-6090 is hsa-miR-609 0, miR-4730 is hsa-miR-4730, and miR-7106-5 p is hsa-miR-7106-5p, and miR-3196 is hsa-miR-31 96, miR-5698 is hsa-miR-5698, and miR-6087 is hsa-miR-6087 and miR-4665-5p are hsa-miR-4665 -5p, miR-8059 is hsa-miR-8059, and miR-6 The device described in (17), wherein 879-5p is hsa-miR-6879-5p. (19) The nucleic acid is a polynucleotide shown in (k) to (o) below: (k) a base sequence represented by any one of SEQ ID NOs: 190 to 214 or the base sequence a polynucleotide consisting of a base sequence in which u is t, a variant thereof, a derivative thereof, or a fragment thereof containing 5 or more consecutive bases; (l) a polynucleotide comprising a nucleotide sequence represented by any one of SEQ ID NOs: 190 to 214; (m) a base sequence represented by any one of SEQ ID NOs: 190 to 214 or the base sequence a polynucleotide consisting of a base sequence complementary to the base sequence in which u is t, a variant thereof, its derivatives or fragments containing 15 or more consecutive bases; (n) a base sequence represented by any one of SEQ ID NOs: 190 to 214 or the base sequence a polynucleotide comprising a base sequence complementary to the base sequence in which u is t; (o) a polynucleotide that binds to any one of the polynucleotides (k) to (n) under stringent conditions; hybridizing polynucleotides, The device according to (17) or (18), which is a polynucleotide selected from the group consisting of Chair. (20) The device is a device for measurement by hybridization technology. The device according to any one of (11) to (19). (21) The method according to (20), wherein the hybridization technique is a nucleic acid array technique. device. (22) The device comprises: At least one polynucleotide capable of specifically binding to each of at least two selected polynucleotides. The device according to any one of (11) to (21), comprising at least two nucleic acids. (23) The kit according to any one of (1) to (10) or (11) to (22). The expression level of a target nucleic acid in a specimen of a subject is measured using the device described in The expression levels measured in the test samples and the control expression levels measured in the test samples were compared to determine whether the test sample was affected by esophageal cancer. In vitro evaluation of whether a patient has esophageal cancer or is free of esophageal cancer 1. A method for detecting esophageal cancer, comprising: (24) The method according to (23), wherein the subject is a human. (25) The method according to (23) or (24), wherein the sample is blood, serum, or plasma. <Terminology> Terms used herein have the following definitions.

[0018] Nucleotide, polynucleotide, DNA, RNA, etc. are abbreviated as "base sequence" Guidelines for the Preparation of Specifications Including Sequences or Amino Acid Sequences" (edited by the Japan Patent Office) and and conventions in the art shall be followed.

[0019] As used herein, the term "polynucleotide" refers to RNA, DNA, and RNA / DNA ( The term "DNA" refers to nucleic acids that include both cDNA and chimeric DNA. The above-mentioned RNA includes total DNA, genomic DNA, and synthetic DNA. l RNA, mRNA, rRNA, miRNA, siRNA, snoRNA, snRNA , including both non-coding RNA and synthetic RNA. As used herein, "synthetic DNA" and "synthetic RNA" refer to a DNA having a predetermined base sequence (natural sequence). or a non-natural sequence), using an automated nucleic acid synthesizer, for example. As used herein, the term "non-natural sequence" refers to DNA and RNA that have been artificially produced. is intended to mean a sequence that differs from the native sequence, e.g., by one or more nucleotides. a sequence containing substitutions, deletions, insertions, and / or additions of one or more modifications (i.e., mutant sequences); The term "sequence" as used herein also encompasses sequences containing nucleotides (i.e., modified sequences), etc. In general, polynucleotide is used interchangeably with nucleic acid.

[0020] As used herein, the term "fragment" refers to a fragment having a continuous partial base sequence of a polynucleotide. It is a polynucleotide having 15 or more bases, preferably 17 or more bases, and more preferably 1 It is desirable that the length be 9 bases or more.

[0021] As used herein, the term "gene" refers not only to RNA and double-stranded DNA, but also to any of the components thereof. Each strand contains a single strand of DNA, such as the positive strand (or sense strand) or the complementary strand (or antisense strand). The term "subject matter" is intended to encompass all subjects, and is not particularly limited by its length. Therefore, in this specification, unless otherwise specified, the term "gene" refers to human genomic DNA. Double-stranded DNA containing the positive strand, single-stranded DNA (positive strand), and single-stranded DNA having a sequence complementary to the positive strand A (complementary strand, including cDNA), microRNA (miRNA), and fragments thereof, The term "gene" also includes a specific base sequence (or sequence Not only are genes represented by numbers, but also the RNAs and biological functionally equivalent RNAs, e.g., homologs (i.e., homologs or orthologs), It also includes "nucleic acids" that encode variants, such as genetic polymorphisms, and derivatives. The "nucleic acid" encoding the mutant or derivative specifically includes a nucleic acid encoding the stringent A nucleotide sequence represented by any one of SEQ ID NOs: 1 to 700, or a salt thereof, under mild conditions. The base sequence has a base sequence that hybridizes with the complementary sequence of the base sequence in which u is t. The term "gene" does not refer to any specific functional region. It may include, for example, an expression control region, a coding region, an exon, or an intron. Furthermore, a "gene" may be contained within a cell, or may be released outside the cell and exist independently. They may also be encapsulated in small vesicles called exosomes.

[0022] As used herein, "exosome" refers to a lipid bilayer membrane-enclosed vesicle secreted from a cell. Exosomes are derived from multivesicular endosomes and release RNA into the extracellular environment. They may contain biological substances such as DNA, genes, and proteins. It is known that the protein is contained in body fluids such as blood, serum, plasma, and lymph. Exosomes are also commonly called "exosomes."

[0023] As used herein, the term "transcription product" refers to a RNA synthesized using the DNA sequence of a gene as a template. A refers to the region called the promoter upstream of the gene where RNA polymerase is located. and binds to the 3' end of the DNA, and binds a ribonucleotide to the 3' end so that it is complementary to the base sequence of the DNA. This RNA contains not only the gene itself but also the the region from the transcription start site to the poly(A) sequence, including the coding region, exon or intron The entire sequence is included, all the way to the termini.

[0024] In addition, in this specification, unless otherwise specified, "microRNA (miRNA)" means It is transcribed as a hairpin-like RNA precursor and has RNase III cleavage activity. It is cleaved by sRNA cleavage enzymes and incorporated into a protein complex called RISC, Non-coding RNAs of 15 to 25 bases involved in RNA translational repression are used intentionally. do.

[0025] Furthermore, the term "miRNA" as used herein is a term that is represented by a specific base sequence (or SEQ ID NO: Not only "miRNA" but also the precursors of the "miRNA" (pre-miRNA, pr i-miRNAs), and miRNAs with equivalent biological functions, e.g., homologs (i.e., (i.e., homologs or orthologs), variants such as genetic polymorphisms, and derivatives are also included. Such precursors, homologues, variants or derivatives include, in particular, miRBase r Identified by eluase 20 (http: / / www.mirbase.org / ) Any of SEQ ID NOs: 1 to 700 can be isolated under the stringent conditions described below. has a base sequence that hybridizes with the complementary sequence of any of the specific base sequences represented by Furthermore, "miRNA" as used herein can be used to refer to a specific miRNA. may be the gene product of a miR gene, and such a gene product is a mature miRNP. A (for example, 15 to 25 bases or 19 to 25 bases involved in the translational repression of mRNA as described above) 5 bases, non-coding RNA) or miRNA precursor (e.g., pre- miRNAs (e.g., prim-miRNAs or pri-miRNAs).

[0026] As used herein, the term "probe" refers to RNA generated by gene expression or a probe derived therefrom. Polynucleotides and / or includes polynucleotides complementary thereto.

[0027] As used herein, the term "primer" refers to an RNA generated by gene expression or a primer therefor. A polynucleotide that specifically recognizes and amplifies the polynucleotide from which it is derived and / or The term "complementary strand" refers to a polynucleotide that is complementary to a given strand. The chain) is a base sequence defined by any one of SEQ ID NOs: 1 to 700, or a salt thereof. a full-length polynucleotide sequence consisting of a base sequence in which u is t in the base sequence, or For the partial sequence (for convenience, this is called the positive strand), The term "polynucleotide" refers to a polynucleotide that is in a base-complementary relationship based on the base pairing relationship. However, such a complementary strand is only permitted to form a completely complementary sequence with the base sequence of the target positive strand. It is complementary to the target positive strand to the extent that it can hybridize under stringent conditions. It may have the following structure.

[0028] As used herein, "stringent conditions" refers to conditions under which a nucleic acid probe is highly sensitive to other sequences. to a greater extent than (e.g., the average of background measurements + the standard of background measurements) The conditions are those under which the hybridization occurs with respect to the target sequence with a standard error of at least 2 times the measured value. Stringent conditions are sequence-dependent and depend on the environment in which the hybridization occurs. The stringency of hybridization and / or washing conditions can be controlled. By doing so, target sequences that are 100% complementary to the nucleic acid probe can be identified. Specific examples of "stringent conditions" will be described later.

[0029] As used herein, the term "Tm value" refers to the time at which the double-stranded portion of a polynucleotide is denatured into a single strand. means the temperature at which double-stranded and single-stranded molecules exist in a 1:1 ratio.

[0030] As used herein, the term "variant" refers to a naturally occurring variant of a nucleic acid resulting from polymorphism, mutation, etc. or a variant thereof, or any of the base sequences of SEQ ID NOs: 1 to 700, or the base sequence In the base sequence in which u is t, or in a partial sequence thereof, one or more bases are deleted or substituted. a mutant containing a substitution, addition or insertion, or a nucleotide sequence or a partial sequence thereof, and % or more, approximately 95% or more, approximately 97% or more, approximately 98% or more, or approximately 99% or more identity or a polynucleotide or oligonucleotide containing said base sequence or a partial sequence thereof The term "nucleotide sequence" refers to a nucleic acid that hybridizes to the nucleic acid under stringent conditions as defined above.

[0031] As used herein, "plurality" refers to an integer of about 10, 9, 8, 7, 6, 5, 4, 3, or 2. It means a number.

[0032] As used herein, the term "mutant" refers to a mutation obtained by site-directed mutagenesis or PCR. They can be produced using well-known techniques such as heterotransferase.

[0033] As used herein, "identity" refers to the degree of identity between proteins or Using the gene search system, we decided to search with or without introducing gaps. (Zheng Zhang et al., 2000, J. Comput. Bi ol., Vol. 7, p. 203-214; Altschul, SF et al., 1990, Jou Journal of Molecular Biology, Vol. 215, p403-410 ;Pearson, WR et al., 1988, Proc. Natl. Acad. Sci. USA, Vol. 85, p2444-2448).

[0034] As used herein, the term "derivative" refers to a modified nucleic acid, for example, but not limited to, labeling with a fluorophore or the like. modified nucleotides (e.g., halogenated, alkyl, such as methyl, methoxy, etc.); Reconstruction of nucleotides and bases containing alkoxy, thio, carboxymethyl, etc. groups nucleotides that have undergone double bond saturation, deamination, and substitution of oxygen molecules with sulfur molecules derivatives including PNA (peptide nucleic acid; Niels en, PE et al., 1991, Science, Vol. 254, p. 1497-500), L NA (locked nucleic acid; Obika, S. et al., 1998, T etrahedron Lett., Vol. 39, p. 5401-5404) means.

[0035] As used herein, a polynucleotide selected from the miRNAs that are esophageal cancer markers is The "nucleic acid" capable of specifically binding to a nucleotide is a synthetic or prepared nucleic acid, specifically a " and detecting the presence or absence of esophageal cancer in a subject. For this purpose, whether or not you have esophageal cancer, the degree of your esophageal cancer, whether or not your esophageal cancer has improved, and the degree of improvement. For diagnosing sensitivity to treatment of esophageal cancer, or for preventing, improving or curing esophageal cancer These are used directly or indirectly to screen candidate substances useful in therapeutic treatments. In relation to the onset of esophageal cancer, the sequence number of the esophageal cancer-related gene in vivo, particularly in samples of body fluids such as blood and urine, is 1 to 700 or its cDNA synthetic nucleic acid. nucleotides, oligonucleotides and polynucleotides that can be combined These nucleotides, oligonucleotides and polynucleotides are based on the above properties. As a probe for detecting the above genes expressed in living organisms, tissues, cells, etc. Furthermore, it can be effectively used as a primer for amplifying the above gene expressed in vivo. can be done.

[0036] The term "detection" as used herein means inspection, measurement, detection, or decision support. In addition, the term "evaluation" as used herein refers to test results or measurement results. It is used to include assisting in diagnosis or evaluation based on the above.

[0037] As used herein, a "subject" refers to a human, a primate, including a chimpanzee, a dog, a cat, or the like. Any pet animals, livestock animals such as cows, horses, sheep, goats, rodents such as mice and rats A "healthy individual" also refers to such a mammal, It means an animal that is not affected by the cancer to be detected, i.e., esophageal cancer.

[0038] As used herein, "P" or "P value" refers to the probability that a statistical test will yield a positive result under the null hypothesis. indicates the probability that a statistic more extreme than the statistic calculated from the actual data will be observed. Therefore, the smaller the "P" or "P value," the more significant the difference between the compared subjects.

[0039] As used herein, "sensitivity" refers to the value of (the number of true positives) / (the number of true positives + the number of false negatives). High sensitivity makes it possible to detect esophageal cancer early and to identify the complete cancer site. This leads to a reduction in resection and recurrence rates.

[0040] As used herein, "specificity" means (number of true negatives) / (number of true negatives + number of false positives). If the specificity is high, unnecessary additional testing due to misclassification of healthy subjects as esophageal cancer patients will be avoided. This will prevent the implementation of the procedure, reducing the burden on patients and cutting medical costs.

[0041] In this specification, "accuracy" means the value of (number of true positives + number of true negatives) / (total number of cases). Accuracy indicates the percentage of all samples for which the discrimination results were correct, and is used to evaluate detection performance. This will be the first indicator.

[0042] In this specification, the term "specimen" to be determined, detected or diagnosed refers to a substance that is capable of detecting the occurrence of esophageal cancer, The expression of the gene of the present invention changes with the progression of esophageal cancer and the therapeutic effect on esophageal cancer. Specifically, it refers to the esophageal tissue and the surrounding blood vessels, lymph nodes, and Organs, organs suspected of metastasis, skin, and bodily fluids such as blood, urine, saliva, sweat, and tissue exudates This refers to serum, plasma, and other products prepared from blood, feces, hair, etc. This refers to biological samples, specifically genes such as RNA and miRNA.

[0043] As used herein, "hsa-miR-204-3p gene" or "hsa-miR The term "hsa-miR-204-3p" refers to the hsa-miR consisting of the base sequence represented by SEQ ID NO: 1. -204-3p gene (miRBase Accession No. MIMAT002 2693) and other species homologs or orthologs. The -204-3p gene is Lim LP et al., 2003, Science, Vol. 299, p. The miR-204 gene can be obtained by the method described in 1540. -3p has a hairpin-like structure as its precursor, hsa-mir-204 (mi RBase Accession No. MI0000284, sequence number 215) is known. It is being done.

[0044] As used herein, the term "hsa-miR-1247-3p gene" or "hsa-mi The term "R-1247-3p" refers to an hsa-mR consisting of the base sequence represented by SEQ ID NO: 2. iR-1247-3p gene (miRBase Accession No. MIMAT 0022721) and other species homologs or orthologs. The miR-1247-3p gene was identified in Morin RD et al., 2008, Genome R It can be obtained by the method described in ES, Vol. 18, pp. 610-621. hsa-miR-1247-3p is a precursor of hsa -mir-1247” (miRBase Accession No. MI000638 2, SEQ ID NO: 216) is known.

[0045] As used herein, "hsa-miR-6875-5p gene" or "hsa-mi The term "R-6875-5p" refers to an hsa-m consisting of the base sequence represented by SEQ ID NO: 3. iR-6875-5p gene (miRBase Accession No. MIMAT 0027650) and other species homologs or orthologs. The miR-6875-5p gene is listed in Ladewig E et al., 2012, Genome Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6875-5p" has a hairpin-like structure as its precursor " hsa-mir-6875” (miRBase Accession No. MI002 2722, SEQ ID NO: 217) is known.

[0046] As used herein, "hsa-miR-6857-5p gene" or "hsa-mi The term "R-6857-5p" refers to an hsa-mR consisting of the base sequence represented by SEQ ID NO: 4. iR-6857-5p gene (miRBase Accession No. MIMAT 0027614) and other species homologs or orthologs. The miR-6857-5p gene is listed in Ladewig E et al., 2012, Genome Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6857-5p" has a hairpin-like structure as its precursor. hsa-mir-6857” (miRBase Accession No. MI002 2703, SEQ ID NO: 218) is known.

[0047] As used herein, "hsa-miR-6726-5p gene" or "hsa-mi The term "R-6726-5p" refers to an hsa-mR consisting of the base sequence represented by SEQ ID NO: 5. iR-6726-5p gene (miRBase Accession No. MIMAT 0027353) and other species homologs or orthologs. The miR-6726-5p gene is listed in Ladewig E et al., 2012, Genome Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6726-5p" has a hairpin-like structure as its precursor " hsa-mir-6726” (miRBase Accession No. MI002 2571, SEQ ID NO: 219) is known.

[0048] As used herein, "hsa-miR-3188 gene" or "hsa-miR-3 The term "hsa-miR-318" refers to the hsa-miR-318 consisting of the nucleotide sequence represented by SEQ ID NO: 6. 8 genes (miRBase Accession No. MIMAT0015070) It also includes homologs or orthologs from other species. The gene is described in Stark MS et al., 2010, PLoS One, Vol. 5, e9685 hsa-miR-3188 can be obtained by the method described above. The miRBase Ac receptor has a hairpin-like structure as a precursor protein, hsa-mir-3188 (miRBase Ac Accession No. MI0014232, SEQ ID NO: 220) is known.

[0049] As used herein, "hsa-miR-8069 gene" or "hsa-miR-8 The term "miR-806" refers to the hsa-miR-806 consisting of the nucleotide sequence represented by SEQ ID NO: 7. 9 genes (miRBase Accession No. MIMAT0030996) It also includes homologs or orthologs from other species. The gene is described in Wang HJ et al., 2013, Shock, Vol. 39, p. 480-487 hsa-miR-8069 can be obtained by the method described above. The miRBase Ac receptor has a hairpin-like structure as a carrier. Accession No. MI0025905, SEQ ID NO: 221) is known.

[0050] As used herein, "hsa-miR-4257 gene" or "hsa-miR-4 The term "miR-425" refers to the hsa-miR-425 gene consisting of the nucleotide sequence represented by SEQ ID NO: 8. 7 genes (miRBase Accession No. MIMAT0016878) It also includes homologs or orthologs from other species. The gene is described in Goff LA et al., 2009, PLoS One, Vol. 4, e7192. hsa-miR-4257 can be obtained by the method described above. The miRBase Accelerator has a hairpin-like structure called "hsa-mir-4257" ( Session No. MI0015856, SEQ ID NO: 222) is known.

[0051] As used herein, "hsa-miR-1343-3p gene" or "hsa-mi The term "R-1343-3p" refers to an hsa-m consisting of the base sequence represented by SEQ ID NO: 9. iR-1343-3p gene (miRBase Accession No. MIMAT 0019776) and other species homologs or orthologs. The miR-1343-3p gene is described in Persson H et al., 2011, Cancer Res, Vol. 71, pp. 78-86. sa-miR-1343-3p is a precursor of the hairpin-like structure hsa- mir-1343” (miRBase Accession No. MI0017320 , SEQ ID NO: 223) is known.

[0052] As used herein, "hsa-miR-7108-5p gene" or "hsa-mi The term "R-7108-5p" refers to an hsa- miR-7108-5p gene (miRBase Accession No. MIMA T0028113) and other species homologs or orthologs. -miR-7108-5p gene is Ladewig E et al., 2012, Genome Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-7108-5p" has a hairpin-like structure as its precursor. "hsa-mir-7108" (miRBase Accession No. MI00 22959, SEQ ID NO: 224) is known.

[0053] As used herein, "hsa-miR-6825-5p gene" or "hsa-mi The term "R-6825-5p" refers to an hsa- miR-6825-5p gene (miRBase Accession No. MIMA T0027550) and other species homologs or orthologs. -miR-6825-5p gene is Ladewig E et al., 2012, Genome Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6825-5p" has a hairpin-like structure as its precursor. "hsa-mir-6825" (miRBase Accession No. MI00 22670, SEQ ID NO: 225) is known.

[0054] As used herein, "hsa-miR-7641 gene" or "hsa-miR-7 The term "miR-76" refers to the hsa-miR-76 gene consisting of the nucleotide sequence represented by SEQ ID NO: 12. 41 genes (miRBase Accession No. MIMAT0029782) hsa-miR-7641 and other species homologs or orthologs. Gene: Yoo JK et al., 2013, Arch Pharm Res, Vol. 36, p. 3 53-358. 641" has a hairpin-like structure as its precursor, "hsa-mir-7641-1, hsa-mir-7641-2” (miRBase Accession No.MI0 024975, MI0024976, SEQ ID NOs: 226, 227) are known.

[0055] As used herein, "hsa-miR-3185 gene" or "hsa-miR-3 The term "miR-31" refers to the hsa-miR-31 gene consisting of the nucleotide sequence represented by SEQ ID NO: 13. 85 genes (miRBase Accession No. MIMAT0015065) hsa-miR-3185 and other species homologs or orthologs. The gene is described in Stark MS et al., 2010, PLoS One, vol. 5, e9685. hsa-miR-3185 can be obtained by the method described in the The precursor of "hsa-mir-3185" (miRBase A Accession No. MI0014227, SEQ ID NO: 228) is known.

[0056] As used herein, "hsa-miR-4746-3p gene" or "hsa-mi The term "R-4746-3p" refers to an hsa- miR-4746-3p gene (miRBase Accession No. MIMA T0019881) and other species homologs or orthologs. -miR-4746-3p gene is Persson H et al., 2011, Cancer Res, Vol. 71, pp. 78-86. hsa-miR-4746-3p is a precursor of hsa -mir-4746” (miRBase Accession No. MI001738 5, SEQ ID NO: 229) is known.

[0057] As used herein, "hsa-miR-6791-5p gene" or "hsa-mi The term "R-6791-5p" refers to an hsa- miR-6791-5p gene (miRBase Accession No. MIMA T0027482) and other species homologs or orthologs. -miR-6791-5p gene is Ladewig E et al., 2012, Genome Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6791-5p" has a hairpin-like structure as its precursor. "hsa-mir-6791" (miRBase Accession No. MI00 22636, SEQ ID NO: 230) is known.

[0058] As used herein, "hsa-miR-6893-5p gene" or "hsa-mi The term "R-6893-5p" refers to an hsa- miR-6893-5p gene (miRBase Accession No. MIMA T0027686) and other species homologs or orthologs. -miR-6893-5p gene is Ladewig E et al., 2012, Genome Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6893-5p" has a hairpin-like structure as its precursor. "hsa-mir-6893" (miRBase Accession No. MI00 22740, SEQ ID NO: 231) is known.

[0059] As used herein, "hsa-miR-4433b-3p gene" or "hsa-m The term "iR-4433b-3p" refers to a hs sequence consisting of the nucleotide sequence represented by SEQ ID NO: 17. a-miR-4433b-3p gene (miRBase Accession No. IMAT0030414) and other species homologs or orthologs. The hsa-miR-4433b-3p gene was identified in Ple H et al., 2012, PLoS O NE, Vol. 7, E50746. -miR-4433b-3p has a hairpin-like structure as its precursor, "hsa-m ir-4433b” (miRBase Accession No. MI0025511 , SEQ ID NO: 232) is known.

[0060] As used herein, "hsa-miR-3135b gene" or "hsa-miR- The term "hsa-miR-3135b" refers to the hsa-miR-3135b consisting of the nucleotide sequence represented by SEQ ID NO: 18. 3135b gene (miRBase Accession No. MIMAT00189 85) and other species homologs or orthologs. The 135b gene is described in Jima DD et al., 2010, Blood, Vol. 116, e118- The miR-313 can be obtained by the method described in e127. 5b" has a hairpin-like structure as its precursor, "hsa-mir-3135b" (m iRBase Accession No. MI0016809, sequence number 233) It is being done.

[0061] As used herein, "hsa-miR-6781-5p gene" or "hsa-mi The term "R-6781-5p" refers to an hsa- miR-6781-5p gene (miRBase Accession No. MIMA T0027462) and other species homologs or orthologs. -miR-6781-5p gene is Ladewig E et al., 2012, Genome Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6781-5p" has a hairpin-like structure as its precursor. "hsa-mir-6781" (miRBase Accession No. MI00 22626, SEQ ID NO: 234) is known.

[0062] As used herein, "hsa-miR-1908-5p gene" or "hsa-mi The term "R-1908-5p" refers to an hsa- miR-1908-5p gene (miRBase Accession No. MIMA T0007881) and other species homologs or orthologs. -miR-1908-5p gene is Bar M et al., 2008, Stem Cells It can be obtained by the method described in Vol. 26, pp. 2496-2505. hsa-miR-1908-5p is a precursor of hsa -mir-1908” (miRBase Accession No. MI000832 9, SEQ ID NO: 235) is known.

[0063] As used herein, "hsa-miR-4792 gene" or "hsa-miR-4 The term "miR-47" refers to the hsa-miR-47 gene consisting of the nucleotide sequence represented by SEQ ID NO: 21. 92 genes (miRBase Accession No. MIMAT0019964) hsa-miR-4792 and other species homologs or orthologs. Gene: Persson H et al., 2011, Cancer Res, vol. 71, p. 78 The miR-4792 can be obtained by the method described in Ref. 86. " has a hairpin-like structure as its precursor "hsa-mir-4792 (miRB)" The enzyme (Accession No. MI0017439, SEQ ID NO: 236) is known There are.

[0064] As used herein, "hsa-miR-7845-5p gene" or "hsa-mi The term "R-7845-5p" refers to an hsa- miR-7845-5p gene (miRBase Accession No. MIMA T0030420) and other species homologs or orthologs. -miR-7845-5p gene is Ple H et al., 2012, PLoS One, 7 The miR gene can be obtained by the method described in Vol. -7845-5p is a precursor of hsa-mir-78, which has a hairpin-like structure. 45" (miRBase Accession No. MI0025515, SEQ ID NO: 2 37) is known.

[0065] As used herein, "hsa-miR-4417 gene" or "hsa-miR-4 The term "miR-417" refers to hsa-miR-44 consisting of the nucleotide sequence represented by SEQ ID NO: 23. 17 genes (miRBase Accession No. MIMAT0018929) hsa-miR-4417 and other species homologs or orthologs. Gene: Jima DD et al., 2010, Blood, Vol. 116, e118-e127 Furthermore, "hsa-miR-4417" can be obtained by the method described in Its precursor, "hsa-mir-4417" (miRBase Accession No. MI0016753, SEQ ID NO: 238) is known.

[0066] As used herein, "hsa-miR-3184-5p gene" or "hsa-mi The term "R-3184-5p" refers to an hsa- miR-3184-5p gene (miRBase Accession No. MIMA T0015064) and other species homologs or orthologs. -miR-3184-5p gene is Stark MS et al., 2010, PLoS On It can be obtained by the method described in Vol. E, Vol. 5, E9685. iR-3184-5p is a precursor of the hairpin-like structure known as hsa-mir- 3184" (miRBase Accession No. MI0014226, sequence no. No. 239) is known.

[0067] As used herein, "hsa-miR-1225-5p gene" or "hsa-mi The term "R-1225-5p" refers to an hsa- miR-1225-5p gene (miRBase Accession No. MIMA T0005572) and other species homologs or orthologs. -miR-1225-5p gene, Berezikov E et al., 2007, Mol It can be obtained by the method described in Cell, Vol. 28, pp. 328-336. "hsa-miR-1225-5p" has a hairpin-like structure as its precursor, "h sa-mir-1225” (miRBase Accession No. MI0006 311, SEQ ID NO: 240) is known.

[0068] As used herein, "hsa-miR-1231 gene" or "hsa-miR-1 The term "miR-12" refers to the hsa-miR-12 gene consisting of the nucleotide sequence represented by SEQ ID NO: 26. 31 genes (miRBase Accession No. MIMAT0005586) hsa-miR-1231 and other species homologs or orthologs. Gene: Berezikov E et al., 2007, Mol Cell, vol. 28, p32 The miR-12 can be obtained by the method described in U.S. Pat. No. 8,336,846. 31” has a hairpin-like structure as its precursor, “hsa-mir-1231” (mi RBase Accession No. MI0006321, sequence number 241) is known. It is being done.

[0069] As used herein, "hsa-miR-1225-3p gene" or "hsa-mi The term "R-1225-3p" refers to an hsa- miR-1225-3p gene (miRBase Accession No. MIMA T0005573) and other species homologs or orthologs. -miR-1225-3p gene is Berezikov E et al., 2007, Mol It can be obtained by the method described in Cell, Vol. 28, pp. 328-336. The precursor of "hsa-miR-1225-3p" is "hsa-miR-1225-3p", which has a hairpin-like structure. sa-mir-1225” (miRBase Accession No. MI0006 311, SEQ ID NO: 240) is known.

[0070] As used herein, "hsa-miR-150-3p gene" or "hsa-miR The term "hsa-mi-150-3p" refers to the hsa-mi-150-3p consisting of the base sequence represented by SEQ ID NO: 28. R-150-3p gene (miRBase Accession No. MIMAT00 04610) and other species homologs or orthologs. The R-150-3p gene is described in Lagos-Quintana M et al., 2002, Cur It can be obtained by the method described in rBiol, vol. 12, pp. 735-739. In addition, "hsa-miR-150-3p" has a hairpin-like structure as its precursor " hsa-mir-150” (miRBase Accession No. MI0000 479, SEQ ID NO: 242) is known.

[0071] As used herein, "hsa-miR-4433-3p gene" or "hsa-mi The term "R-4433-3p" refers to an hsa- miR-4433-3p gene (miRBase Accession No. MIMA T0018949) and other species homologs or orthologs. -miR-4433-3p gene is Jima DD et al., 2010, Blood, 11 It can be obtained by the method described in Vol. 6, E118-E127. -miR-4433-3p has a hairpin-like structure as its precursor, "hsa-mi r-4433” (miRBase Accession No. MI0016773, Column number 243) is known.

[0072] As used herein, "hsa-miR-6125 gene" or "hsa-miR-6 The term "miR-61" refers to the hsa-miR-61 gene consisting of the nucleotide sequence represented by SEQ ID NO: 30. 25 genes (miRBase Accession No. MIMAT0024598) hsa-miR-6125 and other species homologs or orthologs. Genes: Smith JL et al., 2012, J Virol, Vol. 86, p5278-5 287. " has a hairpin-like structure as its precursor "hsa-mir-6125" (miRB The enzyme (Accession No. MI0021259, SEQ ID NO: 244) is known There are.

[0073] As used herein, "hsa-miR-4513 gene" or "hsa-miR-4 The term "miR-45" refers to the hsa-miR-45 gene consisting of the nucleotide sequence represented by SEQ ID NO: 31. 13 genes (miRBase Accession No. MIMAT0019050) hsa-miR-4513 and other species homologs or orthologs. Gene: Jima DD et al., 2010, Blood, Vol. 116, e118-e127 Furthermore, "hsa-miR-4513" can be obtained by the method described in Its precursor, "hsa-mir-4513" (miRBase Accession No. MI0016879, SEQ ID NO: 245) is known.

[0074] As used herein, "hsa-miR-6787-5p gene" or "hsa-mi The term "R-6787-5p" refers to an hsa- miR-6787-5p gene (miRBase Accession No. MIMA T0027474) and other species homologs or orthologs. -miR-6787-5p gene is Ladewig E et al., 2012, Genome Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6787-5p" has a hairpin-like structure as its precursor. "hsa-mir-6787" (miRBase Accession No. MI00 22632, SEQ ID NO: 246) is known.

[0075] As used herein, "hsa-miR-6784-5p gene" or "hsa-mi The term "R-6784-5p" refers to an hsa- miR-6784-5p gene (miRBase Accession No. MIMA T0027468) and other species homologs or orthologs. -miR-6784-5p gene is Ladewig E et al., 2012, Genome Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6784-5p" has a hairpin-like structure as its precursor. "hsa-mir-6784" (miRBase Accession No. MI00 22629, SEQ ID NO: 247) is known.

[0076] As used herein, "hsa-miR-615-5p gene" or "hsa-miR The term "hsa-mi-615-5p" refers to the hsa-mi-615-5p consisting of the base sequence represented by SEQ ID NO: 34. R-615-5p gene (miRBase Accession No. MIMAT00 04804) and other species homologs or orthologs. The R-615-5p gene is described in Cummins JM et al., 2006, Proc Natl A method described in Acad Sci USA, Vol. 103, pp. 3687-3692 Furthermore, "hsa-miR-615-5p" can be obtained as its precursor. The hairpin-like structure of "hsa-mir-615" (miRBase Accession No. on No. MI0003628, SEQ ID NO: 248) is known.

[0077] As used herein, "hsa-miR-6765-3p gene" or "hsa-mi The term "R-6765-3p" refers to an hsa- miR-6765-3p gene (miRBase Accession No. MIMA T0027431) and other species homologs or orthologs. -miR-6765-3p gene is Ladewig E et al., 2012, Genome Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6765-3p" has a hairpin-like structure as its precursor. "hsa-mir-6765" (miRBase Accession No. MI00 22610, SEQ ID NO: 249) is known.

[0078] As used herein, "hsa-miR-5572 gene" or "hsa-miR-5 The term "miR-55" refers to the hsa-miR-55 gene consisting of the nucleotide sequence represented by SEQ ID NO: 36. 72 genes (miRBase Accession No. MIMAT0022260) hsa-miR-5572 and other species homologs or orthologs. Gene: Tandon M et al., 2012, Oral Dis, Vol. 18, p127-1 31. Also, "hsa-miR-5572" can be obtained by the method described in Its precursor, "hsa-mir-5572" (miRBa (Accession No. MI0019117, SEQ ID NO: 250) do.

[0079] As used herein, "hsa-miR-6842-5p gene" or "hsa-mi The term "R-6842-5p" refers to an hsa- miR-6842-5p gene (miRBase Accession No. MIMA T0027586) and other species homologs or orthologs. -miR-6842-5p gene is Ladewig E et al., 2012, Genome Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6842-5p" has a hairpin-like structure as its precursor. "hsa-mir-6842" (miRBase Accession No. MI00 22688, SEQ ID NO: 251) is known.

[0080] As used herein, "hsa-miR-8063 gene" or "hsa-miR-8 The term "miR-80" refers to the hsa-miR-80 gene consisting of the nucleotide sequence represented by SEQ ID NO: 38. 63 genes (miRBase Accession No. MIMAT0030990) and other species homologs or orthologs. The gene is described in Wang HJ et al., 2013, Shock, Vol. 39, p480-487. hsa-miR-8063 can be obtained by the method described in the The precursor of "hsa-mir-8063" (miRBase A Accession No. MI0025899, SEQ ID NO: 252) is known.

[0081] As used herein, "hsa-miR-6780b-5p gene" or "hsa-m The term "iR-6780b-5p" refers to a hs sequence consisting of the nucleotide sequence represented by SEQ ID NO: 39. a-miR-6780b-5p gene (miRBase Accession No. IMAT0027572) and other species homologs or orthologs. The hsa-miR-6780b-5p gene is described in Ladewig E et al., 2012, Ge It can be obtained by the method described in Nome Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6780b-5p" has a hairpin-like structure as its precursor. The structure of "hsa-mir-6780b" (miRBase Accession No. o.MI0022681, sequence number 253) is known.

[0082] As used herein, "hsa-miR-187-5p gene" or "hsa-miR The term "hsa-mi-187-5p" refers to the hsa-mi-187-5p consisting of the base sequence represented by SEQ ID NO: 40. R-187-5p gene (miRBase Accession No. MIMAT00 04561) and other species homologs or orthologs. The R-187-5p gene is described in Lim LP et al., 2003, Science, Vol. 299, The miR-18 gene can be obtained by the method described on p. 1540. 7-5p" has a hairpin-like structure as its precursor "hsa-mir-187" (m iRBase Accession No. MI0000274, sequence number 254) It is being done.

[0083] As used herein, "hsa-miR-128-1-5p gene" or "hsa-m The term "iR-128-1-5p" refers to a hs sequence consisting of the nucleotide sequence represented by SEQ ID NO: 41. a-miR-128-1-5p gene (miRBase Accession No. M IMAT0026477) and other species homologs or orthologs. The hsa-miR-128-1-5p gene was identified in Lagos-Quintana M et al., 2 The method described in Curr Biol, Vol. 12, pp. 735-739, 2002, is In addition, "hsa-miR-128-1-5p" can be expressed as a precursor of hsa-miR-128-1-5p. The apin-like structure of "hsa-mir-128-1" (miRBase Accession No. on No. MI0000447, SEQ ID NO: 255) is known.

[0084] As used herein, "hsa-miR-6729-5p gene" or "hsa-mi The term "R-6729-5p" refers to an hsa- miR-6729-5p gene (miRBase Accession No. MIMA T0027359) and other species homologs or orthologs. -miR-6729-5p gene is Ladewig E et al., 2012, Genome Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6729-5p" has a hairpin-like structure as its precursor. "hsa-mir-6729" (miRBase Accession No. MI00 22574, sequence number 256) is known.

[0085] As used herein, "hsa-miR-6741-5p gene" or "hsa-mi The term "R-6741-5p" refers to an hsa- miR-6741-5p gene (miRBase Accession No. MIMA T0027383) and other species homologs or orthologs. -miR-6741-5p gene is Ladewig E et al., 2012, Genome Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6741-5p" has a hairpin-like structure as its precursor. "hsa-mir-6741" (miRBase Accession No. MI00 22586, SEQ ID NO: 257) is known.

[0086] As used herein, "hsa-miR-6757-5p gene" or "hsa-mi The term "R-6757-5p" refers to an hsa- miR-6757-5p gene (miRBase Accession No. MIMA T0027414) and other species homologs or orthologs. -miR-6757-5p gene is Ladewig E et al., 2012, Genome Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6757-5p" has a hairpin-like structure as its precursor. "hsa-mir-6757" (miRBase Accession No. MI00 22602, SEQ ID NO: 258) is known.

[0087] As used herein, "hsa-miR-7110-5p gene" or "hsa-mi The term "R-7110-5p" refers to an hsa- miR-7110-5p gene (miRBase Accession No. MIMA T0028117) and other species homologs or orthologs. -miR-7110-5p gene is Ladewig E et al., 2012, Genome Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-7110-5p" has a hairpin-like structure as its precursor. "hsa-mir-7110" (miRBase Accession No. MI00 22961, SEQ ID NO: 259) is known.

[0088] As used herein, "hsa-miR-7975 gene" or "hsa-miR-7 The term "miR-79" refers to the hsa-miR-79 gene consisting of the nucleotide sequence represented by SEQ ID NO: 46. 75 genes (miRBase Accession No. MIMAT0031178) hsa-miR-7975 and other species homologs or orthologs. Genes are listed in Velthut-Meikas A et al., 2013, Mol Endocri It can be obtained by the method described in the online version of the nol. miR-7975 has a hairpin-like structure as its precursor, hsa-mir-79 75" (miRBase Accession No. MI0025751, SEQ ID NO: 2 60) is known.

[0089] As used herein, "hsa-miR-1233-5p gene" or "hsa-mi The term "R-1233-5p" refers to an hsa- miR-1233-5p gene (miRBase Accession No. MIMA T0022943) and other species homologs or orthologs. -miR-1233-5p gene, Berezikov E et al., 2007, Mol It can be obtained by the method described in Cell, Vol. 28, pp. 328-336. "hsa-miR-1233-5p" is a precursor of "hsa-miR-1233-5p" which has a hairpin-like structure. sa-mir-1233-1, hsa-mir-1233-2” (miRBase Ac Accession No. MI0006323, MI0015973, SEQ ID NO: 261, 2 62) is known.

[0090] As used herein, "hsa-miR-6845-5p gene" or "hsa-mi The term "R-6845-5p" refers to an hsa- miR-6845-5p gene (miRBase Accession No. MIMA T0027590) and other species homologs or orthologs. -miR-6845-5p gene is Ladewig E et al., 2012, Genome Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6845-5p" has a hairpin-like structure as its precursor. "hsa-mir-6845" (miRBase Accession No. MI00 22691, SEQ ID NO: 263) is known.

[0091] As used herein, "hsa-miR-3937 gene" or "hsa-miR-3 The term "miR-39" refers to the hsa-miR-39 consisting of the nucleotide sequence represented by SEQ ID NO: 49. 37 genes (miRBase Accession No. MIMAT0018352) hsa-miR-3937 and other species homologs or orthologs. The gene is described in Liao JY et al., 2010, PLoS One, Vol. 5, e10563. hsa-miR-3937 can be obtained by the method described in the The precursor of "hsa-mir-3937" (miRBase A Accession No. MI0016593, SEQ ID NO: 264) is known.

[0092] As used herein, "hsa-miR-4467 gene" or "hsa-miR-4 The term "miR-467" refers to hsa-miR-44 consisting of the nucleotide sequence represented by SEQ ID NO: 50. 67 genes (miRBase Accession No. MIMAT0018994) hsa-miR-4467 and other species homologs or orthologs. Gene: Jima DD et al., 2010, Blood, Vol. 116, e118-e127 Furthermore, "hsa-miR-4467" can be obtained by the method described in Its precursor, "hsa-mir-4467" (miRBase Accession No. MI0016818, SEQ ID NO: 265) is known.

[0093] As used herein, "hsa-miR-7109-5p gene" or "hsa-mi The term "R-7109-5p" refers to an hsa- miR-7109-5p gene (miRBase Accession No. MIMA T0028115) and other species homologs or orthologs. -miR-7109-5p gene is Ladewig E et al., 2012, Genome Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-7109-5p" has a hairpin-like structure as its precursor. "hsa-mir-7109" (miRBase Accession No. MI00 22960, SEQ ID NO: 266) is known.

[0094] As used herein, "hsa-miR-6088 gene" or "hsa-miR-6 The term "miR-60" refers to the hsa-miR-60 consisting of the nucleotide sequence represented by SEQ ID NO: 52. 88 genes (miRBase Accession No. MIMAT0023713) and other species homologs or orthologs. Gene: Yoo JK et al., 2012, Stem Cells Dev, Vol. 21, p2 The hsa-miR -6088" has a hairpin-like structure as its precursor, "hsa-mir-6088" (miRBase Accession No. MI0020365, SEQ ID NO: 267) is known.

[0095] As used herein, "hsa-miR-6782-5p gene" or "hsa-mi The term "R-6782-5p" refers to an hsa- miR-6782-5p gene (miRBase Accession No. MIMA T0027464) and other species homologs or orthologs. -miR-6782-5p gene is Ladewig E et al., 2012, Genome Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6782-5p" has a hairpin-like structure as its precursor. "hsa-mir-6782" (miRBase Accession No. MI00 22627, SEQ ID NO: 268) is known.

[0096] As used herein, "hsa-miR-5195-3p gene" or "hsa-mi The term "R-5195-3p" refers to an hsa- miR-5195-3p gene (miRBase Accession No. MIMA T0021127) and other species homologs or orthologs. -miR-5195-3p gene, Schotte D et al., 2011, Leukem It can be obtained by the method described in IA, Vol. 25, pp. 1389-1399. "hsa-miR-5195-3p" has a hairpin-like structure as its precursor, "h sa-mir-5195” (miRBase Accession No. MI0018 174, SEQ ID NO: 269) is known.

[0097] As used herein, "hsa-miR-4454 gene" or "hsa-miR-4 The term "miR-454" refers to hsa-miR-44 consisting of the nucleotide sequence represented by SEQ ID NO: 55. 54 genes (miRBase Accession No. MIMAT0018976) hsa-miR-4454 and other species homologs or orthologs. Gene: Jima DD et al., 2010, Blood, Vol. 116, e118-e127 Furthermore, "hsa-miR-4454" can be obtained by the method described in Its precursor, "hsa-mir-4454" (miRBase Accession No. MI0016800, SEQ ID NO: 270) is known.

[0098] As used herein, "hsa-miR-6724-5p gene" or "hsa-mi The term "R-6724-5p" refers to an hsa- miR-6724-5p gene (miRBase Accession No. MIMA T0025856) and other species homologs or orthologs. -miR-6724-5p gene is Li Y et al., 2012, Gene, vol. 497, p. The "hsa-miR- 6724-5p is a precursor of hsa-mir-672, which has a hairpin-like structure. 4" (miRBase Accession No. MI0022559, SEQ ID NO: 27 1) is known.

[0099] As used herein, "hsa-miR-8072 gene" or "hsa-miR-8 The term "miR-80" refers to the hsa-miR-80 having the nucleotide sequence represented by SEQ ID NO: 57. 72 genes (miRBase Accession No. MIMAT0030999) and other species homologs or orthologs. The gene is described in Wang HJ et al., 2013, Shock, Vol. 39, p480-487. hsa-miR-8072 can be obtained by the method described in the The precursor of "hsa-mir-8072" (miRBase A Accession No. MI0025908, SEQ ID NO: 272) is known.

[0100] As used herein, "hsa-miR-4516 gene" or "hsa-miR-4 The term "miR-45" refers to the hsa-miR-45 gene consisting of the nucleotide sequence represented by SEQ ID NO: 58. 16 genes (miRBase Accession No. MIMAT0019053) hsa-miR-4516 and other species homologs or orthologs. Gene: Jima DD et al., 2010, Blood, Vol. 116, e118-e127 Furthermore, "hsa-miR-4516" can be obtained by the method described in Its precursor, "hsa-mir-4516" (miRBase Accession No. MI0016882, SEQ ID NO: 273) is known.

[0101] As used herein, "hsa-miR-6756-5p gene" or "hsa-mi The term "R-6756-5p" refers to an hsa- miR-6756-5p gene (miRBase Accession No. MIMA T0027412) and other species homologs or orthologs. -miR-6756-5p gene is Ladewig E et al., 2012, Genome Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6756-5p" has a hairpin-like structure as its precursor. "hsa-mir-6756" (miRBase Accession No. MI00 22601, SEQ ID NO: 274) is known.

[0102] As used herein, "hsa-miR-4665-3p gene" or "hsa-mi The term "R-4665-3p" refers to an hsa- miR-4665-3p gene (miRBase Accession No. MIMA T0019740) and other species homologs or orthologs. -miR-4665-3p gene is Persson H et al., 2011, Cancer Res, Vol. 71, pp. 78-86. hsa-miR-4665-3p is a precursor of hsa -mir-4665” (miRBase Accession No. MI001729 5, SEQ ID NO: 275) is known.

[0103] As used herein, "hsa-miR-6826-5p gene" or "hsa-mi The term "R-6826-5p" refers to an hsa- miR-6826-5p gene (miRBase Accession No. MIMA T0027552) and other species homologs or orthologs. -miR-6826-5p gene is Ladewig E et al., 2012, Genome Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6826-5p" has a hairpin-like structure as its precursor. "hsa-mir-6826" (miRBase Accession No. MI00 22671, SEQ ID NO: 276) is known.

[0104] As used herein, "hsa-miR-6820-5p gene" or "hsa-mi The term "R-6820-5p" refers to an hsa- miR-6820-5p gene (miRBase Accession No. MIMA T0027540) and other species homologs or orthologs. -miR-6820-5p gene is Ladewig E et al., 2012, Genome Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6820-5p" has a hairpin-like structure as its precursor. "hsa-mir-6820" (miRBase Accession No. MI00 22665, SEQ ID NO: 277) is known.

[0105] As used herein, "hsa-miR-6887-5p gene" or "hsa-mi The term "R-6887-5p" refers to an hsa- miR-6887-5p gene (miRBase Accession No. MIMA T0027674) and other species homologs or orthologs. -miR-6887-5p gene is Ladewig E et al., 2012, Genome Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6887-5p" has a hairpin-like structure as its precursor. "hsa-mir-6887" (miRBase Accession No. MI00 22734, SEQ ID NO: 278) is known.

[0106] As used herein, "hsa-miR-3679-5p gene" or "hsa-mi The term "R-3679-5p" refers to an hsa- miR-3679-5p gene (miRBase Accession No. MIMA T0018104) and other species homologs or orthologs. -miR-3679-5p gene is Creighton CJ et al., 2010, PLo S One, Vol. 5, e9637. sa-miR-3679-5p has a hairpin-like structure as its precursor, hsa- mir-3679” (miRBase Accession No. MI0016080 , SEQ ID NO: 279) is known.

[0107] As used herein, "hsa-miR-7847-3p gene" or "hsa-mi The term "R-7847-3p" refers to an hsa- miR-7847-3p gene (miRBase Accession No. MIMA T0030422) and other species homologs or orthologs. -miR-7847-3p gene is Ple H et al., 2012, PLoS One, 7 The miR gene can be obtained by the method described in Vol. -7847-3p is a precursor of hsa-mir-78, which has a hairpin-like structure. 47" (miRBase Accession No. MI0025517, SEQ ID NO: 2 80) is known.

[0108] As used herein, "hsa-miR-6721-5p gene" or "hsa-mi The term "R-6721-5p" refers to an hsa- miR-6721-5p gene (miRBase Accession No. MIMA T0025852) and other species homologs or orthologs. -miR-6721-5p gene is Li Y et al., 2012, Gene, vol. 497, p. The "hsa-miR- 6721-5p is a precursor of hsa-mir-672, which has a hairpin-like structure. 1" (miRBase Accession No. MI0022556, SEQ ID NO: 28 1) is known.

[0109] As used herein, "hsa-miR-3622a-5p gene" or "hsa-m The term "iR-3622a-5p" refers to a hs sequence consisting of the nucleotide sequence represented by SEQ ID NO: 67. a-miR-3622a-5p gene (miRBase Accession No. IMAT0018003) and other species homologs or orthologs. The hsa-miR-3622a-5p gene was identified in Witten D et al., 2010, BMC It can be obtained by the method described in Biol, Vol. 8, p. 58. -miR-3622a-5p has a hairpin-like structure as its precursor, "hsa-m ir-3622a” (miRBase Accession No. MI0016013 , SEQ ID NO: 282) is known.

[0110] As used herein, "hsa-miR-939-5p gene" or "hsa-miR The term "hsa-mi-939-5p" refers to the hsa-mi-939-5p consisting of the base sequence represented by SEQ ID NO: 68. R-939-5p gene (miRBase Accession No. MIMAT00 04982) and other species homologs or orthologs. The R-939-5p gene is described in Lui WO et al., 2007, Cancer Res, 67 It can be obtained by the method described in Vol. 1, pp. 6031-6043. -miR-939-5p has a hairpin-like structure as its precursor, "hsa-mir -939" (miRBase Accession No. MI0005761, sequence no. No. 283) is known.

[0111] As used herein, "hsa-miR-602 gene" or "hsa-miR-60 The term "miR-602" refers to the hsa-miR-602 gene consisting of the nucleotide sequence represented by SEQ ID NO: 69. gene (miRBase Accession No. MIMAT0003270) and its It includes homologs or orthologs from other species. , Cummins JM et al. 2006, Proc Natl Acad Sci U It can be obtained by the method described in SA, Vol. 103, pp. 3687-3692. In addition, "hsa-miR-602" has a hairpin-like structure as its precursor, "hsa -mir-602” (miRBase Accession No. MI0003615 , SEQ ID NO: 284) is known.

[0112] As used herein, "hsa-miR-7977 gene" or "hsa-miR-7 The term "miR-79" refers to the hsa-miR-79 consisting of the nucleotide sequence represented by SEQ ID NO: 70. 77 genes (miRBase Accession No. MIMAT0031180) hsa-miR-7977 and other species homologs or orthologs. Genes are listed in Velthut-Meikas A et al., 2013, Mol Endocri It can be obtained by the method described in the online version of the nol. miR-7977 has a hairpin-like structure as its precursor, hsa-mir-79 77" (miRBase Accession No. MI0025753, SEQ ID NO: 2 85) is known.

[0113] As used herein, "hsa-miR-6749-5p gene" or "hsa-mi The term "R-6749-5p" refers to an hsa- miR-6749-5p gene (miRBase Accession No. MIMA T0027398) and other species homologs or orthologs. -miR-6749-5p gene is Ladewig E et al., 2012, Genome Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6749-5p" has a hairpin-like structure as its precursor. "hsa-mir-6749" (miRBase Accession No. MI00 22594, SEQ ID NO: 286) is known.

[0114] As used herein, "hsa-miR-1914-3p gene" or "hsa-mi The term "R-1914-3p" refers to an hsa- miR-1914-3p gene (miRBase Accession No. MIMA T0007890) and other species homologs or orthologs. -miR-1914-3p gene is Bar M et al., 2008, Stem Cells It can be obtained by the method described in Vol. 26, pp. 2496-2505. hsa-miR-1914-3p is a precursor of hsa -mir-1914” (miRBase Accession No. MI000833 5, SEQ ID NO: 287) is known.

[0115] As used herein, "hsa-miR-4651 gene" or "hsa-miR-4 The term "miR-46" refers to the hsa-miR-46 consisting of the nucleotide sequence represented by SEQ ID NO: 73. 51 genes (miRBase Accession No. MIMAT0019715) hsa-miR-4651 and other species homologs or orthologs. Gene: Persson H et al., 2011, Cancer Res, vol. 71, p. 78 The miR-4651 gene can be obtained by the method described in Ref. 86. " has a hairpin-like structure as its precursor "hsa-mir-4651 (miRB)" The enzyme (Accession No. MI0017279, SEQ ID NO: 288) is known There are.

[0116] As used herein, "hsa-miR-4695-5p gene" or "hsa-mi The term "R-4695-5p" refers to an hsa- miR-4695-5p gene (miRBase Accession No. MIMA T0019788) and other species homologs or orthologs. -miR-4695-5p gene is Persson H et al., 2011, Cancer Res, Vol. 71, pp. 78-86. hsa-miR-4695-5p is a precursor of hsa -mir-4695” (miRBase Accession No. MI001732 8, SEQ ID NO: 289) is known.

[0117] As used herein, "hsa-miR-6848-5p gene" or "hsa-mi The term "R-6848-5p" refers to an hsa- miR-6848-5p gene (miRBase Accession No. MIMA T0027596) and other species homologs or orthologs. -miR-6848-5p gene is Ladewig E et al., 2012, Genome Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6848-5p" has a hairpin-like structure as its precursor. "hsa-mir-6848" (miRBase Accession No. MI00 22694, SEQ ID NO: 290) is known.

[0118] As used herein, "hsa-miR-1228-3p gene" or "hsa-mi The term "R-1228-3p" refers to an hsa- miR-1228-3p gene (miRBase Accession No. MIMA T0005583) and other species homologs or orthologs. -miR-1228-3p gene is Berezikov E et al., 2007, Mol It can be obtained by the method described in Cell, Vol. 28, pp. 328-336. "hsa-miR-1228-3p" has a hairpin-like structure as its precursor, "h sa-mir-1228” (miRBase Accession No. MI0006 318, SEQ ID NO: 291) is known.

[0119] As used herein, "hsa-miR-642b-3p gene" or "hsa-mi The term "R-642b-3p" refers to an hsa- miR-642b-3p gene (miRBase Accession No. MIMA T0018444) and other species homologs or orthologs. -miR-642b-3p gene, Witten D et al., 2010, BMC Bio hsa-miR can be obtained by the method described in Vol. 1, Vol. 8, p. 58. -642b-3p is a precursor of hsa-mir-64, which has a hairpin-like structure. 2b" (miRBase Accession No. MI0016685, SEQ ID NO: 2 92) is known.

[0120] As used herein, "hsa-miR-6746-5p gene" or "hsa-mi The term "R-6746-5p" refers to an hsa- miR-6746-5p gene (miRBase Accession No. MIMA T0027392) and other species homologs or orthologs. -miR-6746-5p gene is Ladewig E et al., 2012, Genome Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6746-5p" has a hairpin-like structure as its precursor. "hsa-mir-6746" (miRBase Accession No. MI00 22591, sequence number 293) is known.

[0121] As used herein, "hsa-miR-3620-5p gene" or "hsa-mi The term "R-3620-5p" refers to an hsa- miR-3620-5p gene (miRBase Accession No. MIMA T0022967) and other species homologs or orthologs. -miR-3620-5p gene, Witten D et al., 2010, BMC Bio hsa-miR can be obtained by the method described in Vol. 1, Vol. 8, p. 58. -3620-5p is a precursor of hsa-mir-36, which has a hairpin-like structure. 20" (miRBase Accession No. MI0016011, SEQ ID NO: 2 94) is known.

[0122] As used herein, "hsa-miR-3131 gene" or "hsa-miR-3 The term "hsa-miR-31" refers to the hsa-miR-31 consisting of the nucleotide sequence represented by SEQ ID NO: 80. 31 genes (miRBase Accession No. MIMAT0014996) hsa-miR-3131 and other species homologs or orthologs. The gene is described in Stark MS et al., 2010, PLoS One, vol. 5, e9685. hsa-miR-3131 can be obtained by the method described in the The precursor, "hsa-mir-3131" (miRBase A Accession No. MI0014151, SEQ ID NO: 295) is known.

[0123] As used herein, "hsa-miR-6732-5p gene" or "hsa-mi The term "R-6732-5p" refers to an hsa- miR-6732-5p gene (miRBase Accession No. MIMA T0027365) and other species homologs or orthologs. -miR-6732-5p gene is Ladewig E et al., 2012, Genome Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6732-5p" has a hairpin-like structure as its precursor. "hsa-mir-6732" (miRBase Accession No. MI00 22577, SEQ ID NO: 296) is known.

[0124] As used herein, "hsa-miR-7113-3p gene" or "hsa-mi The term "R-7113-3p" refers to an hsa- miR-7113-3p gene (miRBase Accession No. MIMA T0028124) and other species homologs or orthologs. -miR-7113-3p gene is Ladewig E et al., 2012, Genome Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-7113-3p" has a hairpin-like structure as its precursor. "hsa-mir-7113" (miRBase Accession No. MI00 22964, SEQ ID NO: 297) is known.

[0125] As used herein, "hsa-miR-23a-3p gene" or "hsa-miR The term "hsa-mi-23a-3p" refers to the hsa-mi-23a-3p consisting of the base sequence represented by SEQ ID NO: 83. R-23a-3p gene (miRBase Accession No. MIMAT00 00078) and other species homologs or orthologs. The R-23a-3p gene is described in Lagos-Quintana M et al., 2001, Sci It can be obtained by the method described in Ref., Vol. 294, pp. 853-858. In addition, "hsa-miR-23a-3p" has a hairpin-like structure as its precursor, "hsa-miR-23a-3p". sa-mir-23a” (miRBase Accession No. MI00000 79, SEQ ID NO: 298) is known.

[0126] As used herein, "hsa-miR-3154 gene" or "hsa-miR-3 The term "hsa-miR-31" refers to the hsa-miR-31 having the nucleotide sequence represented by SEQ ID NO: 84. 54 genes (miRBase Accession No. MIMAT0015028) hsa-miR-3154 and other species homologs or orthologs. Genes: Berezikov E et al., 2006, Genome Res, vol. 16, p. It can be obtained by the method described in 1289-1298. R-3154 has a hairpin-like structure as its precursor, hsa-mir-3154 ” (miRBase Accession No. MI0014182, SEQ ID NO: 299 ) is known.

[0127] As used herein, "hsa-miR-4723-5p gene" or "hsa-mi The term "R-4723-5p" refers to an hsa- miR-4723-5p gene (miRBase Accession No. MIMA T0019838) and other species homologs or orthologs. -miR-4723-5p gene is Persson H et al., 2011, Cancer Res, Vol. 71, pp. 78-86. hsa-miR-4723-5p is a precursor of hsa -mir-4723” (miRBase Accession No. MI001735 9, SEQ ID NO: 300) is known.

[0128] As used herein, "hsa-miR-3663-3p gene" or "hsa-mi The term "R-3663-3p" refers to an hsa- miR-3663-3p gene (miRBase Accession No. MIMA T0018085) and other species homologs or orthologs. -miR-3663-3p gene, Liao JY et al., 2010, PLoS One , Vol. 5, E10563. iR-3663-3p is a precursor of the hairpin-like structure known as hsa-mir- 3663" (miRBase Accession No. MI0016064, sequence no. No. 301) is known.

[0129] As used herein, "hsa-miR-4734 gene" or "hsa-miR-4 The term "miR-47" refers to the hsa-miR-47 consisting of the nucleotide sequence represented by SEQ ID NO: 87. 34 genes (miRBase Accession No. MIMAT0019859) and other species homologs or orthologs. Gene: Persson H et al., 2011, Cancer Res, vol. 71, p. 78 The miR-4734 gene can be obtained by the method described in Ref. 86. " has a hairpin-like structure as its precursor, "hsa-mir-4734 (miRB) The enzyme (Accession No. MI0017371, SEQ ID NO: 302) is known There are.

[0130] As used herein, "hsa-miR-6816-5p gene" or "hsa-mi The term "R-6816-5p" refers to an hsa- miR-6816-5p gene (miRBase Accession No. MIMA T0027532) and other species homologs or orthologs. -miR-6816-5p gene is Ladewig E et al., 2012, Genome Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6816-5p" has a hairpin-like structure as its precursor. "hsa-mir-6816" (miRBase Accession No. MI00 22661, SEQ ID NO: 303) is known.

[0131] As used herein, "hsa-miR-4442 gene" or "hsa-miR-4 The term "miR-442" refers to hsa-miR-44 consisting of the nucleotide sequence represented by SEQ ID NO: 89. 42 genes (miRBase Accession No. MIMAT0018960) hsa-miR-4442 and other species homologs or orthologs. Gene: Jima DD et al., 2010, Blood, Vol. 116, e118-e127 Furthermore, "hsa-miR-4442" can be obtained by the method described in Its precursor, "hsa-mir-4442" (miRBase Accession No. MI0016785, SEQ ID NO: 304) is known.

[0132] As used herein, "hsa-miR-4476 gene" or "hsa-miR-4 The term "miR-476" refers to hsa-miR-44 consisting of the nucleotide sequence represented by SEQ ID NO: 90. 76 genes (miRBase Accession No. MIMAT0019003) hsa-miR-4476 and other species homologs or orthologs. Gene: Jima DD et al., 2010, Blood, Vol. 116, e118-e127 Furthermore, "hsa-miR-4476" can be obtained by the method described in Its precursor, "hsa-mir-4476" (miRBase Accession No. MI0016828, SEQ ID NO: 305) is known.

[0133] As used herein, "hsa-miR-423-5p gene" or "hsa-miR The term "hsa-mi-423-5p" refers to the hsa-mi-423-5p consisting of the base sequence represented by SEQ ID NO: 91. R-423-5p gene (miRBase Accession No. MIMAT00 04748) and other species homologs or orthologs. The R-423-5p gene was synthesized by Kasashima K et al., 2004, Biochem The method described in Biophys Res Commun, Vol. 322, p. 403-410 Furthermore, "hsa-miR-423-5p" can be obtained by The miRBase Accession No. 10000266666 (hsa-mir-423) forms a hairpin-like structure. ion No. MI0001445, SEQ ID NO: 306) is known.

[0134] As used herein, "hsa-miR-1249 gene" or "hsa-miR-1 The term "miR-12" refers to the hsa-miR-12 having the nucleotide sequence represented by SEQ ID NO: 92. 49 genes (miRBase Accession No. MIMAT0005901) hsa-miR-1249 and other species homologs or orthologs. Genes: Morin RD et al., 2008, Genome Res, vol. 18, p. 610 The miR-124 can be obtained by the method described in JP-621-1621. 9” has a hairpin-like structure as its precursor, “hsa-mir-1249” (miR Base Accession No. MI0006384, SEQ ID NO: 307) is known are.

[0135] As used herein, "hsa-miR-6515-3p gene" or "hsa-mi The term "R-6515-3p" refers to an hsa- miR-6515-3p gene (miRBase Accession No. MIMA T0025487) and other species homologs or orthologs. -miR-6515-3p gene is Joyce CE et al., 2011, Hum Mol It can be obtained by the method described in Genet, Vol. 20, pp. 4025-4040. In addition, "hsa-miR-6515-3p" has a hairpin-like structure as its precursor. Take "hsa-mir-6515" (miRBase Accession No. MI 0022227, SEQ ID NO: 308) is known.

[0136] As used herein, "hsa-miR-887-3p gene" or "hsa-miR The term "hsa-mi-887-3p" refers to the hsa-mi-887-3p consisting of the base sequence represented by SEQ ID NO: 94. R-887-3p gene (miRBase Accession No. MIMAT00 04951) and other species homologs or orthologs. The R-887-3p gene was identified in Berezikov E et al., 2006, Genome R It can be obtained by the method described in ES, Vol. 16, pp. 1289-1298. "hsa-miR-887-3p" is a precursor of "hs a-mir-887” (miRBase Accession No. MI000556 2, SEQ ID NO: 309) is known.

[0137] As used herein, "hsa-miR-4741 gene" or "hsa-miR-4 The term "miR-47" refers to the hsa-miR-47 consisting of the nucleotide sequence represented by SEQ ID NO: 95. 41 genes (miRBase Accession No. MIMAT0019871) hsa-miR-4741 and other species homologs or orthologs. Gene: Persson H et al., 2011, Cancer Res, vol. 71, p. 78 The miR-4741 can be obtained by the method described in Ref. 86. " has a hairpin-like structure as its precursor, "hsa-mir-4741" (miRB The enzyme (Accession No. MI0017379, SEQ ID NO: 310) is known There are.

[0138] As used herein, "hsa-miR-6766-3p gene" or "hsa-mi The term "R-6766-3p" refers to an hsa- miR-6766-3p gene (miRBase Accession No. MIMA T0027433) and other species homologs or orthologs. -miR-6766-3p gene is Ladewig E et al., 2012, Genome Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6766-3p" has a hairpin-like structure as its precursor. "hsa-mir-6766" (miRBase Accession No. MI00 22611, SEQ ID NO: 311) is known.

[0139] As used herein, "hsa-miR-4673 gene" or "hsa-miR-4 The term "miR-46" refers to the hsa-miR-46 consisting of the nucleotide sequence represented by SEQ ID NO: 97. 73 genes (miRBase Accession No. MIMAT0019755) hsa-miR-4673 and other species homologs or orthologs. Gene: Persson H et al., 2011, Cancer Res, vol. 71, p. 78 The miR-4673 can be obtained by the method described in Ref. 86. " has a hairpin-like structure as its precursor "hsa-mir-4673 (miRB)" The enzyme (Accession No. MI0017304, SEQ ID NO: 312) is known There are.

[0140] As used herein, "hsa-miR-6779-5p gene" or "hsa-mi The term "R-6779-5p" refers to an hsa- miR-6779-5p gene (miRBase Accession No. MIMA T0027458) and other species homologs or orthologs. -miR-6779-5p gene is Ladewig E et al., 2012, Genome Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6779-5p" has a hairpin-like structure as its precursor. "hsa-mir-6779" (miRBase Accession No. MI00 22624, SEQ ID NO: 313) is known.

[0141] As used herein, "hsa-miR-4706 gene" or "hsa-miR-4 The term "miR-47" refers to the hsa-miR-47 consisting of the nucleotide sequence represented by SEQ ID NO: 99. 06 gene (miRBase Accession No. MIMAT0019806) hsa-miR-4706 and other species homologs or orthologs. Gene: Persson H et al., 2011, Cancer Res, vol. 71, p. 78 The miR-4706 can be obtained by the method described in Ref. " has a hairpin-like structure as its precursor "hsa-mir-4706" (miRB The enzyme (Accession No. MI0017339, SEQ ID NO: 314) is known There are.

[0142] As used herein, "hsa-miR-1268b gene" or "hsa-miR- The term "hsa-miR 1268b" refers to the hsa-miR consisting of the nucleotide sequence represented by SEQ ID NO: 100. -1268b gene (miRBase Accession No. MIMAT0018 925) and other species homologs or orthologs. The 1268b gene is described in Jima DD et al., 2010, Blood, Vol. 116, e118 The miR-12 gene can be obtained by the method described in Ref. 68b" has a hairpin-like structure as its precursor, "hsa-mir-1268b" ( miRBase Accession No. MI0016748, SEQ ID NO: 315) It is known.

[0143] As used herein, "hsa-miR-4632-5p gene" or "hsa-mi The term "R-4632-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 101. -miR-4632-5p gene (miRBase Accession No. MIM AT0022977) and other species homologs or orthologs. The a-miR-4632-5p gene is described in Persson H et al., 2011, Cancer It can be obtained by the method described in J. R. Res., Vol. 71, pp. 78-86. "hsa-miR-4632-5p" is a precursor of "hs a-mir-4632” (miRBase Accession No. MI00172 59, SEQ ID NO: 316) is known.

[0144] As used herein, "hsa-miR-3197 gene" or "hsa-miR-3 The term "miR-3" refers to the hsa-miR-3 having the nucleotide sequence represented by SEQ ID NO: 102. 197 genes (miRBase Accession No. MIMAT0015082 ) and other species homologs or orthologs. 7 genes are listed in Stark MS et al., 2010, PLoS One, Vol. 5, e9685 hsa-miR-3197 can be obtained by the method described in The precursor of the hairpin-like structure "hsa-mir-3197" (miRBase Accession No. MI0014245, SEQ ID NO: 317) is known.

[0145] As used herein, "hsa-miR-6798-5p gene" or "hsa-mi The term "R-6798-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 103. -miR-6798-5p gene (miRBase Accession No. MIM AT0027496) and other species homologs or orthologs. The a-miR-6798-5p gene was identified in Ladewig E et al., 2012, Genom e Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6798-5p" has a hairpin-like structure as its precursor. The miRBase Accession No. "hsa-mir-6798" (MIRBase Accession No. MI0 022643, SEQ ID NO: 318) is known.

[0146] As used herein, "hsa-miR-711 gene" or "hsa-miR-71 The term "miR-711" refers to the hsa-miR-711 consisting of the nucleotide sequence represented by SEQ ID NO: 104. Gene (miRBase Accession No. MIMAT0012734) and This includes homologs or orthologs of other species. Artzi S et al., 2008, BMC Bioinformatics, Vol. 9, p. 39. In addition, "hsa-miR-711" can be obtained by the method described in , and its precursor, "hsa-mir-711" (miRBase Accession No. MI0012488, SEQ ID NO: 319) is known.

[0147] As used herein, "hsa-miR-6840-3p gene" or "hsa-mi The term "R-6840-3p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 105. -miR-6840-3p gene (miRBase Accession No. MIM AT0027583) and other species homologs or orthologs. The a-miR-6840-3p gene was identified in Ladewig E et al., 2012, Genom e Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6840-3p" has a hairpin-like structure as its precursor. The miRBase Accession No. MI0 022686, SEQ ID NO: 320) is known.

[0148] As used herein, "hsa-miR-6763-5p gene" or "hsa-mi The term "R-6763-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 106. -miR-6763-5p gene (miRBase Accession No. MIM AT0027426) and other species homologs or orthologs. The a-miR-6763-5p gene was identified in Ladewig E et al., 2012, Genom e Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6763-5p" has a hairpin-like structure as its precursor. The miRBase Accession No. MI0 022608, SEQ ID NO: 321) is known.

[0149] As used herein, "hsa-miR-6727-5p gene" or "hsa-mi The term "R-6727-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 107. -miR-6727-5p gene (miRBase Accession No. MIM AT0027355) and other species homologs or orthologs. The a-miR-6727-5p gene was identified in Ladewig E et al., 2012, Genom e Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6727-5p" has a hairpin-like structure as its precursor. The miRBase Accession No. MI0 022572, SEQ ID NO: 322) is known.

[0150] As used herein, "hsa-miR-371a-5p gene" or "hsa-mi The term "R-371a-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 108. -miR-371a-5p gene (miRBase Accession No. MIM AT0004687) and other species homologs or orthologs. The a-miR-371a-5p gene was identified in Suh MR et al., 2004, Dev Biol It can be obtained by the method described in Vol. 270, pp. 488-498. sa-miR-371a-5p has a hairpin-like structure as its precursor, hsa- mir-371a” (miRBase Accession No. MI0000779 , SEQ ID NO: 323) is known.

[0151] As used herein, "hsa-miR-6824-5p gene" or "hsa-mi The term "R-6824-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 109. -miR-6824-5p gene (miRBase Accession No. MIM AT0027548) and other species homologs or orthologs. The a-miR-6824-5p gene was identified in Ladewig E et al., 2012, Genom e Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6824-5p" has a hairpin-like structure as its precursor. The miRBase Accession No. "hsa-mir-6824" (MIRBase Accession No. MI0 022669, SEQ ID NO: 324) is known.

[0152] As used herein, "hsa-miR-4648 gene" or "hsa-miR-4 The term "miR-4" refers to the hsa-miR-4 having the nucleotide sequence represented by SEQ ID NO: 110. 648 genes (miRBase Accession No. MIMAT0019710 ) and other species homologs or orthologs. 8 genes are listed in Persson H et al., 2011, Cancer Res, Vol. 71, p. 7 8-86. 8” has a hairpin-like structure as its precursor, “hsa-mir-4648” (miR Base Accession No. MI0017275, SEQ ID NO: 325) is known are.

[0153] As used herein, "hsa-miR-1227-5p gene" or "hsa-mi The term "R-1227-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 111. -miR-1227-5p gene (miRBase Accession No. MIM AT0022941) and other species homologs or orthologs. The a-miR-1227-5p gene was identified in Berezikov E et al., 2007, Mol It can be obtained by the method described in Cell, Vol. 28, pp. 328-336. In addition, "hsa-miR-1227-5p" has a hairpin-like structure as its precursor " hsa-mir-1227” (miRBase Accession No. MI000 6316, SEQ ID NO: 326) is known.

[0154] As used herein, "hsa-miR-564 gene" or "hsa-miR-56 The term "miR-564" refers to hsa-miR-564 consisting of the nucleotide sequence represented by SEQ ID NO: 112. Gene (miRBase Accession No. MIMAT0003228) and This includes homologs or orthologs of the hsa-miR-564 gene in other species. Cummins JM et al., 2006, Proc Natl Acad Sci U SA, Vol. 103, pp. 3687-3692. In addition, "hsa-miR-564" has a precursor "hs" that has a hairpin-like structure. a-mir-564” (miRBase Accession No. MI000357 0, SEQ ID NO: 327) is known.

[0155] As used herein, "hsa-miR-3679-3p gene" or "hsa-mi The term "R-3679-3p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 113. -miR-3679-3p gene (miRBase Accession No. MIM AT0018105) and other species homologs or orthologs. The a-miR-3679-3p gene is described in Creighton CJ et al., 2010, PL It can be obtained by the method described in OS One, Vol. 5, e9637. hsa-miR-3679-3p is a precursor of hsa -mir-3679” (miRBase Accession No. MI001608 0, SEQ ID NO: 279) is known.

[0156] As used herein, "hsa-miR-2861 gene" or "hsa-miR-2 The term "miR-2" refers to the hsa-miR-2 having the nucleotide sequence represented by SEQ ID NO: 114. 861 genes (miRBase Accession No. MIMAT0013802 ) and other species homologs or orthologs. 1 gene, Li H et al., 2009, J Clin Invest, Vol. 119, p. 36 The "hsa-miR- 2861" has a hairpin-like structure as its precursor, "hsa-mir-2861" ( miRBase Accession No. MI0013006, SEQ ID NO: 328) It is known.

[0157] As used herein, "hsa-miR-6737-5p gene" or "hsa-mi The term "R-6737-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 115. -miR-6737-5p gene (miRBase Accession No. MIM AT0027375) and other species homologs or orthologs. The a-miR-6737-5p gene was identified in Ladewig E et al., 2012, Genom e Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6737-5p" has a hairpin-like structure as its precursor. The miRBase Accession No. "hsa-mir-6737" (MIRBase Accession No. MI0 022582, SEQ ID NO: 329) is known.

[0158] As used herein, "hsa-miR-575 gene" or "hsa-miR-57 The term "miR-575" refers to the hsa-miR-575 consisting of the nucleotide sequence represented by SEQ ID NO: 116. Gene (miRBase Accession No. MIMAT0003240) and This includes homologs or orthologs of the hsa-miR-575 gene in other organisms. Cummins JM et al., 2006, Proc Natl Acad Sci U SA, Vol. 103, pp. 3687-3692. In addition, "hsa-miR-575" has a precursor "hs" that has a hairpin-like structure. a-mir-575” (miRBase Accession No. MI000358 2, SEQ ID NO: 330) is known.

[0159] As used herein, "hsa-miR-4725-3p gene" or "hsa-mi The term "R-4725-3p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 117. -miR-4725-3p gene (miRBase Accession No. MIM AT0019844) and other species homologs or orthologs. The a-miR-4725-3p gene is Persson H et al., 2011, Cancer It can be obtained by the method described in J. R. Res., Vol. 71, pp. 78-86. "hsa-miR-4725-3p" is a precursor of "hs a-mir-4725” (miRBase Accession No. MI00173 62, SEQ ID NO: 331) is known.

[0160] As used herein, "hsa-miR-6716-5p gene" or "hsa-mi The term "R-6716-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 118. -miR-6716-5p gene (miRBase Accession No. MIM AT0025844) and other species homologs or orthologs. The a-miR-6716-5p gene is described in Li Y et al., 2012, Gene, Vol. 497, The miR gene can be obtained by the method described on pages 330-335. -6716-5p is a precursor of hsa-mir-67, which has a hairpin-like structure. 16" (miRBase Accession No. MI0022550, SEQ ID NO: 3 32) is known.

[0161] As used herein, "hsa-miR-4675 gene" or "hsa-miR-4 The term "miR-4" refers to the hsa-miR-4 having the nucleotide sequence represented by SEQ ID NO: 119. 675 genes (miRBase Accession No. MIMAT0019757 ) and other species homologs or orthologs. 5 genes are listed in Persson H et al., 2011, Cancer Res, Vol. 71, p. 7 8-86. 5” has a hairpin-like structure as its precursor, “hsa-mir-4675” (miR Base Accession No. MI0017306, SEQ ID NO: 333) is known are.

[0162] As used herein, "hsa-miR-1915-3p gene" or "hsa-mi The term "R-1915-3p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 120. -miR-1915-3p gene (miRBase Accession No. MIM AT0007892) and other species homologs or orthologs. The a-miR-1915-3p gene is described in Bar M et al., 2008, Stem Cell It can be obtained by the method described in Vol. 26, pp. 2496-2505. "hsa-miR-1915-3p" is a precursor of "hs a-mir-1915” (miRBase Accession No. MI00083 36, SEQ ID NO: 334) is known.

[0163] As used herein, "hsa-miR-671-5p gene" or "hsa-miR The term "HSA-M-671-5p" refers to the HSA-M-M consisting of the base sequence represented by SEQ ID NO: 121. iR-671-5p gene (miRBase Accession No. MIMAT0 003880) and other species homologs or orthologs. The iR-671-5p gene is described in Berezikov E et al., 2006, Genome Res, Vol. 16, pp. 1289-1298. In addition, "hsa-miR-671-5p" has a hairpin-like structure as its precursor, "hsa-miR-671-5p." sa-mir-671” (miRBase Accession No. MI00037 60, SEQ ID NO: 335) is known.

[0164] As used herein, "hsa-miR-3656 gene" or "hsa-miR-3 The term "miR-3" refers to the hsa-miR-3 having the nucleotide sequence represented by SEQ ID NO: 122. 656 genes (miRBase Accession No. MIMAT0018076 ) and other species homologs or orthologs. 6 genes are listed in Meiri E et al., 2010, Nucleic Acids Res, 3 It can be obtained by the method described in Vol. 8, pp. 6234-6246. a-miR-3656 has a hairpin-like structure as its precursor, hsa-mir- 3656" (miRBase Accession No. MI0016056, sequence no. No. 336) is known.

[0165] As used herein, "hsa-miR-6722-3p gene" or "hsa-mi The term "R-6722-3p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 123. -miR-6722-3p gene (miRBase Accession No. MIM AT0025854) and other species homologs or orthologs. The a-miR-6722-3p gene is described in Li Y et al., 2012, Gene, Vol. 497, The miR gene can be obtained by the method described on pages 330-335. -6722-3p is a precursor of hsa-mir-67, which has a hairpin-like structure. 22" (miRBase Accession No. MI0022557, SEQ ID NO: 3 37) is known.

[0166] As used herein, "hsa-miR-4707-5p gene" or "hsa-mi The term "R-4707-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 124. -miR-4707-5p gene (miRBase Accession No. MIM AT0019807) and other species homologs or orthologs. The a-miR-4707-5p gene is Persson H et al., 2011, Cancer It can be obtained by the method described in J. R. Res., Vol. 71, pp. 78-86. "hsa-miR-4707-5p" is a precursor of "hs a-mir-4707” (miRBase Accession No. MI00173 40, SEQ ID NO: 338) is known.

[0167] As used herein, "hsa-miR-4449 gene" or "hsa-miR-4 The term "miR-449" refers to the hsa-miR-4 consisting of the nucleotide sequence represented by SEQ ID NO: 125. 449 genes (miRBase Accession No. MIMAT0018968 ) and other species homologs or orthologs. 9 genes are described in Jima DD et al., 2010, Blood, Vol. 116, e118-e12 7. Furthermore, "hsa-miR-4449" can be obtained by the method described in , and its precursor, "hsa-mir-4449" (miRBas e Accession No. MI0016792, SEQ ID NO: 339) is known .

[0168] As used herein, "hsa-miR-1202 gene" or "hsa-miR-1 The term "hsa-miR-1 202" refers to the hsa-miR-1 202 having the nucleotide sequence represented by SEQ ID NO: 126. 202 genes (miRBase Accession No. MIMAT0005865 ) and other species homologs or orthologs. 2 gene, Marton S et al., 2008, Leukemia, Vol. 22, p330- 338. " has a hairpin-like structure as its precursor, "hsa-mir-1202" (miRB The enzyme (Accession No. MI0006334, SEQ ID NO: 340) is known There are.

[0169] As used herein, "hsa-miR-4649-5p gene" or "hsa-mi The term "R-4649-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 127. -miR-4649-5p gene (miRBase Accession No. MIM AT0019711) and other species homologs or orthologs. The a-miR-4649-5p gene is Persson H et al., 2011, Cancer It can be obtained by the method described in J. R. Res., Vol. 71, pp. 78-86. "hsa-miR-4649-5p" is a precursor of "hs a-mir-4649” (miRBase Accession No. MI00172 76, SEQ ID NO: 341) is known.

[0170] As used herein, "hsa-miR-744-5p gene" or "hsa-miR The term "HSA-M-744-5p" refers to the HSA-M-M consisting of the base sequence represented by SEQ ID NO: 128. iR-744-5p gene (miRBase Accession No. MIMAT0 004945) and other species homologs or orthologs. The iR-744-5p gene is described in Berezikov E et al., 2006, Genome Res, Vol. 16, pp. 1289-1298. In addition, "hsa-miR-744-5p" has a hairpin-like structure as its precursor, "hsa-miR-744-5p." sa-mir-744” (miRBase Accession No. MI00055 59, SEQ ID NO: 342) is known.

[0171] As used herein, "hsa-miR-642a-3p gene" or "hsa-mi The term "R-642a-3p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 129. -miR-642a-3p gene (miRBase Accession No. MIM AT0020924) and other species homologs or orthologs. The a-miR-642a-3p gene was identified in Cummins JM et al., 2006, Proc Natl Acad Sci USA, Vol. 103, p3687-3692 Furthermore, "hsa-miR-642a-3p" can be obtained by the method described above. The precursor of the miR gene, hsa-mir-642a (miRBase Accession No. MI0003657, SEQ ID NO: 343) is known.

[0172] As used herein, "hsa-miR-451a gene" or "hsa-miR-4 The term "miR-4" refers to the hsa-miR-4 having the nucleotide sequence represented by SEQ ID NO: 130. 51a gene (miRBase Accession No. MIMAT0001631 ) and other species homologs or orthologs. a gene is Altuvia Y et al., 2005, Nucleic Acids Res It can be obtained by the method described in Vol. 33, pp. 2697-2706. hsa-miR-451a is a precursor of hsa-miR, which has a hairpin-like structure. r-451a” (miRBase Accession No. MI0001729, Column number 344) is known.

[0173] As used herein, "hsa-miR-6870-5p gene" or "hsa-mi The term "R-6870-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 131. -miR-6870-5p gene (miRBase Accession No. MIM AT0027640) and other species homologs or orthologs. The a-miR-6870-5p gene was identified in Ladewig E et al., 2012, Genom e Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6870-5p" has a hairpin-like structure as its precursor. The miRBase Accession No. "hsa-mir-6870" (MIRBase Accession No. MI0 022717, SEQ ID NO: 345) is known.

[0174] As used herein, "hsa-miR-4443 gene" or "hsa-miR-4 The term "miR-443" refers to the hsa-miR-4 consisting of the nucleotide sequence represented by SEQ ID NO: 132. 443 genes (miRBase Accession No. MIMAT0018961 ) and other species homologs or orthologs. 3 genes are described in Jima DD et al., 2010, Blood, Vol. 116, e118-e12 7. Furthermore, "hsa-miR-4443" can be obtained by the method described in , and its precursor, "hsa-mir-4443" (miRBas e Accession No. MI0016786, SEQ ID NO: 346) is known .

[0175] As used herein, "hsa-miR-6808-5p gene" or "hsa-mi The term "R-6808-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 133. -miR-6808-5p gene (miRBase Accession No. MIM AT0027516) and other species homologs or orthologs. The a-miR-6808-5p gene was identified in Ladewig E et al., 2012, Genom e Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6808-5p" has a hairpin-like structure as its precursor. The miRBase Accession No. MI0 022653, SEQ ID NO: 347) is known.

[0176] As used herein, "hsa-miR-4728-5p gene" or "hsa-mi The term "R-4728-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 134. -miR-4728-5p gene (miRBase Accession No. MIM AT0019849) and other species homologs or orthologs. The a-miR-4728-5p gene is Persson H et al., 2011, Cancer It can be obtained by the method described in J. R. Res., Vol. 71, pp. 78-86. "hsa-miR-4728-5p" is a precursor of "hs a-mir-4728” (miRBase Accession No. MI00173 65, SEQ ID NO: 348) is known.

[0177] As used herein, "hsa-miR-937-5p gene" or "hsa-miR The term "HSA-M-937-5p" refers to the HSA-M-M consisting of the base sequence represented by SEQ ID NO: 135. iR-937-5p gene (miRBase Accession No. MIMAT0 022938) and other species homologs or orthologs. The iR-937-5p gene is described in Lui WO et al., 2007, Cancer Res, 6 It can be obtained by the method described in Vol. 7, pp. 6031-6043. a-miR-937-5p has a hairpin-like structure as its precursor, hsa-mi r-937" (miRBase Accession No. MI0005759, sequence Number 349) is known.

[0178] As used herein, "hsa-miR-135a-3p gene" or "hsa-mi The term "R-135a-3p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 136. -miR-135a-3p gene (miRBase Accession No. MIM AT0004595) and other species homologs or orthologs. The a-miR-135a-3p gene was identified by Lagos-Quintana M et al., 2002 The method described in Curr Biol, Vol. 12, p. 735-739 In addition, "hsa-miR-135a-3p" has a hairpin-like structure as its precursor. The structure of "hsa-mir-135a-1" (miRBase Accession No. No. MI0000452, SEQ ID NO: 350) are known.

[0179] As used herein, "hsa-miR-663b gene" or "hsa-miR-6 The term "hsa-miR-6 63b" refers to the hsa-miR-6 consisting of the nucleotide sequence represented by SEQ ID NO: 137. 63b gene (miRBase Accession No. MIMAT0005867 ) and other species homologs or orthologs. b gene, Takada S et al., 2008, Leukemia, Vol. 22, p. 1274 The miR-66 can be obtained by the method described in JP-1278-1278. 3b” has a hairpin-like structure as its precursor “hsa-mir-663b” (mi RBase Accession No. MI0006336, sequence number 351) is known. It is being done.

[0180] As used herein, "hsa-miR-1343-5p gene" or "hsa-mi The term "R-1343-5p" refers to an hsa consisting of the base sequence represented by SEQ ID NO: 138. -miR-1343-5p gene (miRBase Accession No. MIM AT0027038) and other species homologs or orthologs. The a-miR-1343-5p gene is Persson H et al., 2011, Cancer It can be obtained by the method described in J. R. Res., Vol. 71, pp. 78-86. "hsa-miR-1343-5p" is a precursor of "hs a-mir-1343” (miRBase Accession No. MI00173 20, SEQ ID NO: 223) is known.

[0181] As used herein, "hsa-miR-6822-5p gene" or "hsa-mi The term "R-6822-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 139. -miR-6822-5p gene (miRBase Accession No. MIM AT0027544) and other species homologs or orthologs. The a-miR-6822-5p gene was identified in Ladewig E et al., 2012, Genom e Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6822-5p" has a hairpin-like structure as its precursor. The miRBase Accession No. MI0 022667, SEQ ID NO: 352) is known.

[0182] As used herein, "hsa-miR-6803-5p gene" or "hsa-mi The term "R-6803-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 140. -miR-6803-5p gene (miRBase Accession No. MIM AT0027506) and other species homologs or orthologs. The a-miR-6803-5p gene was identified in Ladewig E et al., 2012, Genom e Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6803-5p" has a hairpin-like structure as its precursor. The miRBase Accession No. MI0 022648, SEQ ID NO: 353) is known.

[0183] As used herein, "hsa-miR-6805-3p gene" or "hsa-mi The term "R-6805-3p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 141. -miR-6805-3p gene (miRBase Accession No. MIM AT0027511) and other species homologs or orthologs. The a-miR-6805-3p gene was identified in Ladewig E et al., 2012, Genom e Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6805-3p" has a hairpin-like structure as its precursor. The miRBase Accession No. MI0 022650, SEQ ID NO: 354) is known.

[0184] As used herein, "hsa-miR-128-2-5p gene" or "hsa-m The term "iR-128-2-5p" refers to a nucleic acid sequence consisting of the base sequence represented by SEQ ID NO: 142. sa-miR-128-2-5p gene (miRBase Accession No. MIMAT0031095) and other species homologs or orthologs. The hsa-miR-128-2-5p gene was reported by Lagos-Quintana M et al. By the method described in Curr Biol, vol. 12, pp. 735-739, 2002. Furthermore, "hsa-miR-128-2-5p" can be obtained as its precursor. The hairpin-like structure of "hsa-mir-128-2" (miRBase Accession No. ion No. MI0000727, SEQ ID NO: 355) is known.

[0185] As used herein, "hsa-miR-4640-5p gene" or "hsa-mi The term "R-4640-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 143. -miR-4640-5p gene (miRBase Accession No. MIM AT0019699) and other species homologs or orthologs. The a-miR-4640-5p gene is Persson H et al., 2011, Cancer It can be obtained by the method described in J. R. Res., Vol. 71, pp. 78-86. "hsa-miR-4640-5p" is a precursor of "hs a-mir-4640” (miRBase Accession No. MI00172 67, SEQ ID NO: 356) is known.

[0186] As used herein, "hsa-miR-1469 gene" or "hsa-miR-1 The term "miR-1" refers to the hsa-miR-1 gene consisting of the nucleotide sequence represented by SEQ ID NO: 144. 469 genes (miRBase Accession No. MIMAT0007347 ) and other species homologs or orthologs. 9 genes are Kawaji H et al., 2008, BMC Genomics, Vol. 9, p. 1 57. Also, "hsa-miR-1469" can be obtained by the method described in The precursor of miRBa is “hsa-mir-1469” (miRBa), which has a hairpin-like structure. (Accession No. MI0007074, SEQ ID NO: 357) do.

[0187] As used herein, "hsa-miR-92a-2-5p gene" or "hsa-m The term "iR-92a-2-5p" refers to a nucleic acid sequence consisting of the nucleic acid sequence represented by SEQ ID NO: 145. sa-miR-92a-2-5p gene (miRBase Accession No. MIMAT0004508) and other species homologs or orthologs The hsa-miR-92a-2-5p gene was identified in Mourelatos Z et al., 2002 The method described in Genes Dev, Vol. 16, pp. 720-728 In addition, "hsa-miR-92a-2-5p" can be synthesized as a hairpin miR-92a precursor. The miRBase Accession No. 100001266644 (hsa-mir-92a-2) has a similar structure to the miR-10001266644 (hsa-mir-92a-2) (miRBase Accession No. 100001266444). No. MI0000094, SEQ ID NO: 358) are known.

[0188] As used herein, "hsa-miR-3940-5p gene" or "hsa-mi The term "R-3940-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 146. -miR-3940-5p gene (miRBase Accession No. MIM AT0019229) and other species homologs or orthologs. The a-miR-3940-5p gene was identified in Liao JY et al., 2010, PLoS On It can be obtained by the method described in Vol. E, Vol. 5, E10563. miR-3940-5p has a hairpin-like structure as its precursor, hsa-mir -3940" (miRBase Accession No. MI0016597, sequence Number 359) is known.

[0189] As used herein, "hsa-miR-4281 gene" or "hsa-miR-4 The term "miR-4" refers to the hsa-miR-4 having the nucleotide sequence represented by SEQ ID NO: 147. 281 genes (miRBase Accession No. MIMAT0016907 ) and other species homologs or orthologs. 1 gene is described in Goff LA et al., 2009, PLoS One, Vol. 4, e7192. hsa-miR-4281 can be obtained by the method described in the The precursor of "hsa-mir-4281" (miRBase A Accession No. MI0015885, SEQ ID NO: 360) is known.

[0190] As used herein, "hsa-miR-1260b gene" or "hsa-miR- The term "hsa-miR 1260b" refers to the hsa-miR consisting of the nucleotide sequence represented by SEQ ID NO: 148. -1260b gene (miRBase Accession No. MIMAT0015 041) and other species homologs or orthologs. The 1260b gene is described in Stark MS et al., 2010, PLoS One, Vol. 5, e9 685. b" has a hairpin-like structure as its precursor, "hsa-mir-1260b" (mi RBase Accession No. MI0014197, sequence number 361) It is being done.

[0191] As used herein, "hsa-miR-4758-5p gene" or "hsa-mi The term "R-4758-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 149. -miR-4758-5p gene (miRBase Accession No. MIM AT0019903) and other species homologs or orthologs. The a-miR-4758-5p gene is Persson H et al., 2011, Cancer It can be obtained by the method described in J. R. Res., Vol. 71, pp. 78-86. "hsa-miR-4758-5p" is a precursor of "hs a-mir-4758” (miRBase Accession No. MI00173 99, SEQ ID NO: 362) is known.

[0192] As used herein, "hsa-miR-1915-5p gene" or "hsa-mi The term "R-1915-5p" refers to an hsa consisting of the base sequence represented by SEQ ID NO: 150. -miR-1915-5p gene (miRBase Accession No. MIM AT0007891) and other species homologs or orthologs. The a-miR-1915-5p gene is described in Bar M et al., 2008, Stem Cell It can be obtained by the method described in Vol. 26, pp. 2496-2505. "hsa-miR-1915-5p" is a precursor of "hs a-mir-1915” (miRBase Accession No. MI00083 36, SEQ ID NO: 334) is known.

[0193] As used herein, "hsa-miR-5001-5p gene" or "hsa-mi The term "R-5001-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 151. -miR-5001-5p gene (miRBase Accession No. MIM AT0021021) and other species homologs or orthologs. The a-miR-5001-5p gene is a member of the RNA B It can be obtained by the method described in IOL, Vol. 8, pp. 378-383. hsa-miR-5001-5p is a precursor of hsa -mir-5001” (miRBase Accession No. MI001786 7, SEQ ID NO: 363) is known.

[0194] As used herein, "hsa-miR-4286 gene" or "hsa-miR-4 The term "miR-4" refers to the hsa-miR-4 gene consisting of the nucleotide sequence represented by SEQ ID NO: 152. 286 genes (miRBase Accession No. MIMAT0016916 ) and other species homologs or orthologs. The six genes are described in Goff LA et al., 2009, PLoS One, Vol. 4, e7192. hsa-miR-4286 can be obtained by the method described in the The precursor of "hsa-mir-4286" (miRBase A Accession No. MI0015894, SEQ ID NO: 364) is known.

[0195] As used herein, "hsa-miR-6126 gene" or "hsa-miR-6 The term "hsa-miR-6" refers to the hsa-miR-6 consisting of the nucleotide sequence represented by SEQ ID NO: 153. 126 genes (miRBase Accession No. MIMAT0024599 ) and other species homologs or orthologs. 6 genes are listed in Smith JL et al., 2012, J Virol, Vol. 86, p5278- The miR-612 can be obtained by the method described in 5287. 6” has a hairpin-like structure as its precursor, “hsa-mir-6126” (miR Base Accession No. MI0021260, SEQ ID NO: 365) is known are.

[0196] As used herein, "hsa-miR-6789-5p gene" or "hsa-mi The term "R-6789-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 154. -miR-6789-5p gene (miRBase Accession No. MIM AT0027478) and other species homologs or orthologs. The a-miR-6789-5p gene was identified in Ladewig E et al., 2012, Genom e Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6789-5p" has a hairpin-like structure as its precursor. The miRBase Accession No. MI0 022634, SEQ ID NO: 366) is known.

[0197] As used herein, "hsa-miR-4459 gene" or "hsa-miR-4 The term "miR-4" refers to the hsa-miR-4 consisting of the nucleotide sequence represented by SEQ ID NO: 155. 459 genes (miRBase Accession No. MIMAT0018981 ) and other species homologs or orthologs. 9 genes are described in Jima DD et al., 2010, Blood, Vol. 116, e118-e12 7. Furthermore, "hsa-miR-4459" can be obtained by the method described in , and its precursor, "hsa-mir-4459" (miRBas e Accession No. MI0016805, SEQ ID NO: 367) is known .

[0198] As used herein, "hsa-miR-1268a gene" or "hsa-miR- The term "hsa-miR 1268a" refers to the hsa-miR consisting of the nucleotide sequence represented by SEQ ID NO: 156. -1268a gene (miRBase Accession No. MIMAT0005 922) and other species homologs or orthologs. The 1268a gene is Morin RD et al., 2008, Genome Res, vol. 18 It can be obtained by the method described in the "hsa-mi" R-1268a is a precursor of hsa-mir-126, which has a hairpin-like structure. 8a" (miRBase Accession No. MI0006405, SEQ ID NO: 3 68) is known.

[0199] As used herein, "hsa-miR-6752-5p gene" or "hsa-mi The term "R-6752-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 157. -miR-6752-5p gene (miRBase Accession No. MIM AT0027404) and other species homologs or orthologs. The a-miR-6752-5p gene was identified in Ladewig E et al., 2012, Genom e Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6752-5p" has a hairpin-like structure as its precursor. The miRBase Accession No. MI0 022597, SEQ ID NO: 369) is known.

[0200] As used herein, "hsa-miR-6131 gene" or "hsa-miR-6 The term "miR-6" refers to the hsa-miR-6 consisting of the nucleotide sequence represented by SEQ ID NO: 158. 131 genes (miRBase Accession No. MIMAT0024615 ) and other species homologs or orthologs. 1 gene, Dannemann M et al., 2012, Genome Biol Evo It can be obtained by the method described in "hs a-miR-6131 has a hairpin-like structure as its precursor, hsa-mir- 6131" (miRBase Accession No. MI0021276, sequence no. No. 370) is known.

[0201] As used herein, "hsa-miR-6800-5p gene" or "hsa-mi The term "R-6800-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 159. -miR-6800-5p gene (miRBase Accession No. MIM AT0027500) and other species homologs or orthologs. The a-miR-6800-5p gene was identified in Ladewig E et al., 2012, Genom e Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6800-5p" has a hairpin-like structure as its precursor. The miRBase Accession No. MI0 022645, SEQ ID NO: 371) is known.

[0202] As used herein, "hsa-miR-4532 gene" or "hsa-miR-4 The term "miR-4" refers to the hsa-miR-4 having the nucleotide sequence represented by SEQ ID NO: 160. 532 genes (miRBase Accession No. MIMAT0019071 ) and other species homologs or orthologs. 2 genes are described in Jima DD et al., 2010, Blood, Vol. 116, e118-e12 7. Furthermore, "hsa-miR-4532" can be obtained by the method described in , and its precursor, "hsa-mir-4532" (miRBas e Accession No. MI0016899, SEQ ID NO: 372) is known .

[0203] As used herein, "hsa-miR-6872-3p gene" or "hsa-mi The term "R-6872-3p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 161. -miR-6872-3p gene (miRBase Accession No. MIM AT0027645) and other species homologs or orthologs. The a-miR-6872-3p gene was identified in Ladewig E et al., 2012, Genom e Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6872-3p" has a hairpin-like structure as its precursor. The miRBase Accession No. MI0 022719, SEQ ID NO: 373) is known.

[0204] As used herein, "hsa-miR-718 gene" or "hsa-miR-71 The term "miR-718" refers to hsa-miR-718 consisting of the nucleotide sequence represented by SEQ ID NO: 162. Gene (miRBase Accession No. MIMAT0012735) and This includes homologs or orthologs of other species. Artzi S et al., 2008, BMC Bioinformatics, Vol. 9, p. 39. In addition, "hsa-miR-718" can be obtained by the method described in , and its precursor, "hsa-mir-718" (miRBase Accession No. MI0012489, SEQ ID NO: 374) is known.

[0205] As used herein, "hsa-miR-6769a-5p gene" or "hsa-m The term "iR-6769a-5p" refers to a nucleic acid sequence consisting of the base sequence represented by SEQ ID NO: 163. sa-miR-6769a-5p gene (miRBase Accession No. MIMAT0027438) and other species homologs or orthologs The hsa-miR-6769a-5p gene is a member of the G Obtained by the method described in Enome Res, Vol. 22, pp. 1634-1645 In addition, "hsa-miR-6769a-5p" can be synthesized as a hairpin miR-6769a-5p precursor. The miRBase Accession No. 100001266444 (hsa-mir-6769a) has a similar structure to the miRBase Accession No. 100001266444 (hsa-mir-6769a) No. MI0022614, SEQ ID NO: 375) is known.

[0206] As used herein, "hsa-miR-4707-3p gene" or "hsa-mi The term "R-4707-3p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 164. -miR-4707-3p gene (miRBase Accession No. MIM AT0019808) and other species homologs or orthologs. The a-miR-4707-3p gene is a gene encoding miR-4707-3p. It can be obtained by the method described in J. R. Res., Vol. 71, pp. 78-86. "hsa-miR-4707-3p" is a precursor of "hs a-mir-4707” (miRBase Accession No. MI00173 40, SEQ ID NO: 338) is known.

[0207] As used herein, "hsa-miR-6765-5p gene" or "hsa-mi The term "R-6765-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 165. -miR-6765-5p gene (miRBase Accession No. MIM AT0027430) and other species homologs or orthologs. The a-miR-6765-5p gene was identified in Ladewig E et al., 2012, Genom e Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6765-5p" has a hairpin-like structure as its precursor. The miRBase Accession No. "hsa-mir-6765" (MIRBase Accession No. MI0 022610, SEQ ID NO: 249) is known.

[0208] As used herein, "hsa-miR-4739 gene" or "hsa-miR-4 The term "miR-4" refers to the hsa-miR-4 having the nucleotide sequence represented by SEQ ID NO: 166. 739 genes (miRBase Accession No. MIMAT0019868 ) and other species homologs or orthologs. 9 genes are listed in Persson H et al., 2011, Cancer Res, Vol. 71, p. 7 8-86. 9” has a hairpin-like structure as its precursor, “hsa-mir-4739” (miR Base Accession No. MI0017377, SEQ ID NO: 376) is known are.

[0209] As used herein, "hsa-miR-4525 gene" or "hsa-miR-4 The term "miR-4" refers to the hsa-miR-4 having the nucleotide sequence represented by SEQ ID NO: 167. 525 genes (miRBase Accession No. MIMAT0019064 ) and other species homologs or orthologs. 5 genes are described in Jima DD et al., 2010, Blood, Vol. 116, e118-e12 7. Furthermore, "hsa-miR-4525" can be obtained by the method described in , and its precursor, "hsa-mir-4525" (miRBas e Accession No. MI0016892, SEQ ID NO: 377) is known .

[0210] As used herein, "hsa-miR-4270 gene" or "hsa-miR-4 The term "miR-4" refers to the hsa-miR-4 having the nucleotide sequence represented by SEQ ID NO: 168. 270 genes (miRBase Accession No. MIMAT0016900 ) and other species homologs or orthologs. The 0 gene is described in Goff LA et al., 2009, PLoS One, Vol. 4, e7192. hsa-miR-4270 can be obtained by the method described in the The precursor of "hsa-mir-4270" (miRBase A Accession No. MI0015878, SEQ ID NO: 378) is known.

[0211] As used herein, "hsa-miR-4534 gene" or "hsa-miR-4 The term "miR-4" refers to the hsa-miR-4 having the nucleotide sequence represented by SEQ ID NO: 169. 534 genes (miRBase Accession No. MIMAT0019073 ) and other species homologs or orthologs. 4 genes are described in Jima DD et al., 2010, Blood, Vol. 116, e118-e12 7. Also, "hsa-miR-4534" can be obtained by the method described in , and its precursor, "hsa-mir-4534" (miRBas e Accession No. MI0016901, SEQ ID NO: 379) is known .

[0212] As used herein, "hsa-miR-6785-5p gene" or "hsa-mi The term "R-6785-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 170. -miR-6785-5p gene (miRBase Accession No. MIM AT0027470) and other species homologs or orthologs. The a-miR-6785-5p gene was identified in Ladewig E et al., 2012, Genom e Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6785-5p" has a hairpin-like structure as its precursor. The miRBase Accession No. MI0 022630, SEQ ID NO: 380) is known.

[0213] As used herein, "hsa-miR-6850-5p gene" or "hsa-mi The term "R-6850-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 171. -miR-6850-5p gene (miRBase Accession No. MIM AT0027600) and other species homologs or orthologs. The a-miR-6850-5p gene was identified in Ladewig E et al., 2012, Genom e Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6850-5p" has a hairpin-like structure as its precursor. The miRBase Accession No. MI0 022696, SEQ ID NO: 381) is known.

[0214] As used herein, "hsa-miR-4697-5p gene" or "hsa-mi The term "R-4697-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 172. -miR-4697-5p gene (miRBase Accession No. MIM AT0019791) and other species homologs or orthologs. The a-miR-4697-5p gene is Persson H et al., 2011, Cancer It can be obtained by the method described in J. R. Res., Vol. 71, pp. 78-86. "hsa-miR-4697-5p" is a precursor of "hs a-mir-4697” (miRBase Accession No. MI00173 30, SEQ ID NO: 382) is known.

[0215] As used herein, "hsa-miR-1260a gene" or "hsa-miR- The term "hsa-miR 1260a" refers to the hsa-miR consisting of the nucleotide sequence represented by SEQ ID NO: 173. -1260a gene (miRBase Accession No. MIMAT0005 911) and other species homologs or orthologs. The 1260a gene is Morin RD et al., 2008, Genome Res, Vol. 18 It can be obtained by the method described in the "hsa-mi" R-1260a is derived from the precursor hsa-mir-126, which has a hairpin-like structure. 0a" (miRBase Accession No. MI0006394, SEQ ID NO: 3 83) is known.

[0216] As used herein, "hsa-miR-4486 gene" or "hsa-miR-4 The term "miR-4" refers to the hsa-miR-4 consisting of the nucleotide sequence represented by SEQ ID NO: 174. 486 genes (miRBase Accession No. MIMAT0019020 ) and other species homologs or orthologs. 6 genes are described in Jima DD et al., 2010, Blood, Vol. 116, e118-e12 7. Also, "hsa-miR-4486" can be obtained by the method described in , and its precursor, "hsa-mir-4486" (miRBas e Accession No. MI0016847, SEQ ID NO: 384) is known .

[0217] As used herein, "hsa-miR-6880-5p gene" or "hsa-mi The term "R-6880-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 175. -miR-6880-5p gene (miRBase Accession No. MIM AT0027660) and other species homologs or orthologs. The a-miR-6880-5p gene was identified in Ladewig E et al., 2012, Genom e Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6880-5p" has a hairpin-like structure as its precursor. The miRBase Accession No. MI0 022727, SEQ ID NO: 385) is known.

[0218] As used herein, "hsa-miR-6802-5p gene" or "hsa-mi The term "R-6802-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 176. -miR-6802-5p gene (miRBase Accession No. MIM AT0027504) and other species homologs or orthologs. The a-miR-6802-5p gene was identified in Ladewig E et al., 2012, Genom e Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6802-5p" has a hairpin-like structure as its precursor. The miRBase Accession No. MI0 022647, SEQ ID NO: 386) is known.

[0219] As used herein, "hsa-miR-6861-5p gene" or "hsa-mi The term "R-6861-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 177. -miR-6861-5p gene (miRBase Accession No. MIM AT0027623) and other species homologs or orthologs. The a-miR-6861-5p gene was identified in Ladewig E et al., 2012, Genom e Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6861-5p" has a hairpin-like structure as its precursor. The miRBase Accession No. MI0 022708, SEQ ID NO: 387) is known.

[0220] As used herein, "hsa-miR-92b-5p gene" or "hsa-miR The term "HSA-M-92b-5p" refers to the HSA-M-M consisting of the base sequence represented by SEQ ID NO: 178. iR-92b-5p gene (miRBase Accession No. MIMAT0 004792) and other species homologs or orthologs. The iR-92b-5p gene is described in Cummins JM et al., 2006, Proc Nat l The method described in Acad Sci USA, Vol. 103, pp. 3687-3692 Furthermore, "hsa-miR-92b-5p" can be obtained by The miRBase Accession No. 10000126666 (hsa-mir-92b) forms a hairpin-like structure. ion No. MI0003560, SEQ ID NO: 388) is known.

[0221] As used herein, "hsa-miR-1238-5p gene" or "hsa-mi The term "R-1238-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 179. -miR-1238-5p gene (miRBase Accession No. MIM AT0022947) and other species homologs or orthologs. The a-miR-1238-5p gene was identified in Berezikov E et al., 2007, Mol It can be obtained by the method described in Cell, Vol. 28, pp. 328-336. In addition, "hsa-miR-1238-5p" has a hairpin-like structure as its precursor " hsa-mir-1238” (miRBase Accession No. MI000 6328, SEQ ID NO: 389) is known.

[0222] As used herein, "hsa-miR-6851-5p gene" or "hsa-mi The term "R-6851-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 180. -miR-6851-5p gene (miRBase Accession No. MIM AT0027602) and other species homologs or orthologs. The a-miR-6851-5p gene was identified in Ladewig E et al., 2012, Genom e Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6851-5p" has a hairpin-like structure as its precursor. The miRBase Accession No. MI0 022697, SEQ ID NO: 390) is known.

[0223] As used herein, "hsa-miR-7704 gene" or "hsa-miR-7 The term "hsa-miR-7" refers to the hsa-miR-7 consisting of the nucleotide sequence represented by SEQ ID NO: 181. 704 genes (miRBase Accession No. MIMAT0030019 ) and other species homologs or orthologs. 4 genes are listed in Swaminathan S et al., 2013, Biochem Bioph obtained by the method described in Phys Res Commun, Vol. 434, pp. 228-234. In addition, "hsa-miR-7704" has a hairpin-like structure as its precursor. The structure of "hsa-mir-7704" (miRBase Accession No. .MI0025240, sequence number 391) is known.

[0224] As used herein, "hsa-miR-149-3p gene" or "hsa-miR The term "HSA-149-3p" refers to the HSA-149-3p consisting of the base sequence represented by SEQ ID NO: 182. iR-149-3p gene (miRBase Accession No. MIMAT0 004609) and other species homologs or orthologs. The iR-149-3p gene is a member of the Lagos-Quintana M et al., 2002, Cu It can be obtained by the method described in rr Biol, Vol. 12, p. 735-739. In addition, "hsa-miR-149-3p" has a hairpin-like structure as its precursor. "hsa-mir-149" (miRBase Accession No. MI000 0478, SEQ ID NO: 392) is known.

[0225] As used herein, "hsa-miR-4689 gene" or "hsa-miR-4 The term "miR-4" refers to the hsa-miR-4 having the nucleotide sequence represented by SEQ ID NO: 183. 689 genes (miRBase Accession No. MIMAT0019778 ) and other species homologs or orthologs. 9 genes are listed in Persson H et al., 2011, Cancer Res, Vol. 71, p. 7 8-86. 9” has a hairpin-like structure as its precursor, “hsa-mir-4689” (miR Base Accession No. MI0017322, SEQ ID NO: 393) is known are.

[0226] As used herein, "hsa-miR-4688 gene" or "hsa-miR-4 The term "miR-4" refers to the hsa-miR-4 having the nucleotide sequence represented by SEQ ID NO: 184. 688 genes (miRBase Accession No. MIMAT0019777 ) and other species homologs or orthologs. 8 genes are listed in Persson H et al., 2011, Cancer Res, Vol. 71, p. 7 8-86. 8” has a hairpin-like structure as its precursor, “hsa-mir-4688” (miR Base Accession No. MI0017321, SEQ ID NO: 394) is known are.

[0227] As used herein, "hsa-miR-125a-3p gene" or "hsa-mi The term "R-125a-3p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 185. -miR-125a-3p gene (miRBase Accession No. MIM AT0004602) and other species homologs or orthologs. The a-miR-125a-3p gene was identified by Lagos-Quintana M et al., 2002 The method described in Curr Biol, Vol. 12, p. 735-739 In addition, "hsa-miR-125a-3p" has a hairpin-like structure as its precursor. The structure of "hsa-mir-125a" (miRBase Accession No. .MI0000469, sequence number 395) is known.

[0228] As used herein, "hsa-miR-23b-3p gene" or "hsa-miR The term "HSA-M-23b-3p" refers to the HSA-M-23b-3p consisting of the base sequence represented by SEQ ID NO: 186. iR-23b-3p gene (miRBase Accession No. MIMAT0 000418) and other species homologs or orthologs. The iR-23b-3p gene is a member of the Lagos-Quintana M et al., 2002, Cu It can be obtained by the method described in rr Biol, Vol. 12, p. 735-739. In addition, "hsa-miR-23b-3p" has a hairpin-like structure as its precursor. "hsa-mir-23b" (miRBase Accession No. MI000 0439, SEQ ID NO: 396) is known.

[0229] As used herein, "hsa-miR-614 gene" or "hsa-miR-61 The term "miR-614" refers to hsa-miR-614 consisting of the nucleotide sequence represented by SEQ ID NO: 187. Gene (miRBase Accession No. MIMAT0003282) and This includes homologs or orthologs of other species. Cummins JM et al., 2006, Proc Natl Acad Sci U SA, Vol. 103, pp. 3687-3692. In addition, "hsa-miR-614" has a precursor "hs" that has a hairpin-like structure. a-mir-614” (miRBase Accession No. MI000362 7, SEQ ID NO: 397) is known.

[0230] As used herein, "hsa-miR-1913 gene" or "hsa-miR-1 The term "miR-1" refers to the hsa-miR-1 gene consisting of the nucleotide sequence represented by SEQ ID NO: 188. 913 genes (miRBase Accession No. MIMAT0007888 ) and other species homologs or orthologs. 3 genes are described in Bar M et al., 2008, Stem Cells, Vol. 26, p2496- The miR-191 gene can be obtained by the method described in 2505. 3” has a hairpin-like structure as its precursor, “hsa-mir-1913” (miR Base Accession No. MI0008334, SEQ ID NO: 398) is known are.

[0231] As used herein, "hsa-miR-16-5p gene" or "hsa-miR- The term "hsa-miR 16-5p" refers to the hsa-miR consisting of the nucleotide sequence represented by SEQ ID NO: 189. -16-5p gene (miRBase Accession No. MIMAT0000 069) and other species homologs or orthologs. The 16-5p gene is described in Lagos-Quintana M et al., 2001, Science e, Vol. 294, pp. 853-858. hsa-miR-16-5p is a precursor of hsa-m ir-16-1, hsa-mir-16-2” (miRBase Accession No. MI0000070, MI0000115, sequence numbers 399 and 400) are known. There are.

[0232] As used herein, "hsa-miR-675-5p gene" or "hsa-miR The term "-675-5p" refers to the hsa-m consisting of the base sequence represented by SEQ ID NO: 190. iR-675-5p gene (miRBase Accession No. MIMAT0 004284) and other species homologs or orthologs. The iR-675-5p gene is Cai X et al., 2007, RNA, Vol. 13, p313- 316. 5p” has a hairpin-like structure as its precursor “hsa-mir-675” (miR Base Accession No. MI0005416, SEQ ID NO: 401) is known are.

[0233] As used herein, "hsa-miR-486-3p gene" or "hsa-miR The term "HSA-M-486-3p" refers to the HSA-M-486-3p consisting of the base sequence represented by SEQ ID NO: 191. iR-486-3p gene (miRBase Accession No. MIMAT0 004762) and other species homologs or orthologs. The iR-486-3p gene is described in Fu H et al., 2005, FEBS Lett, Vol. 579 It can be obtained by the method described in the above, pp. 3849-3854. miR-486-3p has a hairpin-like structure as its precursor, hsa-mir- 486, hsa-mir-486-2” (miRBase Accession No. MI0002470, MI0023622, SEQ ID NOs: 402, 403) are known.

[0234] As used herein, "hsa-miR-6777-5p gene" or "hsa-mi The term "R-6777-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 192. -miR-6777-5p gene (miRBase Accession No. MIM AT0027454) and other species homologs or orthologs. The a-miR-6777-5p gene was identified in Ladewig E et al., 2012, Genom e Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6777-5p" has a hairpin-like structure as its precursor. The miRBase Accession No. MI0 022622, SEQ ID NO: 404) is known.

[0235] As used herein, "hsa-miR-4497 gene" or "hsa-miR-4 The term "miR-4" refers to the hsa-miR-4 consisting of the nucleotide sequence represented by SEQ ID NO: 193. 497 genes (miRBase Accession No. MIMAT0019032 ) and other species homologs or orthologs. 7 genes are described in Jima DD et al., 2010, Blood, Vol. 116, e118-e12 7. Furthermore, "hsa-miR-4497" can be obtained by the method described in , and its precursor, "hsa-mir-4497" (miRBas e Accession No. MI0016859, SEQ ID NO: 405) is known .

[0236] As used herein, "hsa-miR-296-3p gene" or "hsa-miR The term "HSA-M-296-3p" refers to the HSA-M-M consisting of the base sequence represented by SEQ ID NO: 194. iR-296-3p gene (miRBase Accession No. MIMAT0 004679) and other species homologs or orthologs. The iR-296-3p gene was identified in Houbaviy HB et al., 2003, Dev Cel It can be obtained by the method described in "hs a-miR-296-3p has a hairpin-like structure as its precursor, hsa-mi r-296" (miRBase Accession No. MI0000747, sequence Number 406) is known.

[0237] As used herein, "hsa-miR-6738-5p gene" or "hsa-mi The term "R-6738-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 195. -miR-6738-5p gene (miRBase Accession No. MIM AT0027377) and other species homologs or orthologs. The a-miR-6738-5p gene was identified in Ladewig E et al., 2012, Genom e Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6738-5p" has a hairpin-like structure as its precursor. The miRBase Accession No. MI0 022583, SEQ ID NO: 407) is known.

[0238] As used herein, "hsa-miR-4731-5p gene" or "hsa-mi The term "R-4731-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 196. -miR-4731-5p gene (miRBase Accession No. MIM AT0019853) and other species homologs or orthologs. The a-miR-4731-5p gene is described in Persson H et al., 2011, Cancer It can be obtained by the method described in J. R. Res., Vol. 71, pp. 78-86. "hsa-miR-4731-5p" is a precursor of "hs a-mir-4731” (miRBase Accession No. MI00173 68, SEQ ID NO: 408) is known.

[0239] As used herein, "hsa-miR-6889-5p gene" or "hsa-mi The term "R-6889-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 197. -miR-6889-5p gene (miRBase Accession No. MIM AT0027678) and other species homologs or orthologs. The a-miR-6889-5p gene was identified in Ladewig E et al., 2012, Genom e Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6889-5p" has a hairpin-like structure as its precursor. The miRBase Accession No. MI0 022736, SEQ ID NO: 409) is known.

[0240] As used herein, "hsa-miR-6786-5p gene" or "hsa-mi The term "R-6786-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 198. -miR-6786-5p gene (miRBase Accession No. MIM AT0027472) and other species homologs or orthologs. The a-miR-6786-5p gene was identified in Ladewig E et al., 2012, Genom e Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6786-5p" has a hairpin-like structure as its precursor. The miRBase Accession No. "hsa-mir-6786" (MIRBase Accession No. MI0 022631, SEQ ID NO: 410) is known.

[0241] As used herein, "hsa-miR-92a-3p gene" or "hsa-miR The term "hsa-m-92a-3p" refers to the hsa-m-92a-3p consisting of the base sequence represented by SEQ ID NO: 199. iR-92a-3p gene (miRBase Accession No. MIMAT0 000092) and other species homologs or orthologs. The iR-92a-3p gene was identified in Mourelatos Z et al., 2002, Genes Dev, Vol. 16, pp. 720-728. "hsa-miR-92a-3p" is a precursor of "hsa" which has a hairpin-like structure. -mir-92a-1, hsa-mir-92a-2” (miRBase Access ion No. MI0000093, MI0000094, sequence numbers 411 and 358) It is known.

[0242] As used herein, "hsa-miR-4294 gene" or "hsa-miR-4 The term "hsa-miR-4 294" refers to the hsa-miR-4 nucleotide sequence represented by SEQ ID NO: 200. 294 genes (miRBase Accession No. MIMAT0016849 ) and other species homologs or orthologs. The four genes are described in Goff LA et al., 2009, PLoS One, Vol. 4, e7192. hsa-miR-4294 can be obtained by the method described in the The precursor of "hsa-mir-4294" (miRBase A Accession No. MI0015827, SEQ ID NO: 412) is known.

[0243] As used herein, "hsa-miR-4763-3p gene" or "hsa-mi The term "R-4763-3p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 201. -miR-4763-3p gene (miRBase Accession No. MIM AT0019913) and other species homologs or orthologs. The a-miR-4763-3p gene is a gene encoding miR-4763-3p. It can be obtained by the method described in J. R. Res., Vol. 71, pp. 78-86. "hsa-miR-4763-3p" is a precursor of "hs a-mir-4763” (miRBase Accession No. MI00174 04, SEQ ID NO: 413) is known.

[0244] As used herein, "hsa-miR-6076 gene" or "hsa-miR-6 The term "miR-6" refers to the hsa-miR-6 consisting of the nucleotide sequence represented by SEQ ID NO: 202. 076 genes (miRBase Accession No. MIMAT0023701 ) and other species homologs or orthologs. 6 genes are from Voellenkle C et al., 2012, RNA, Vol. 18, p472-4 84. Also, "hsa-miR-6076" The precursor of miRBa is “hsa-mir-6076” (miRBa (Accession No. MI0020353, SEQ ID NO: 414) do.

[0245] As used herein, "hsa-miR-663a gene" or "hsa-miR-6 The term "hsa-miR-6 63a" refers to the hsa-miR-6 consisting of the nucleotide sequence represented by SEQ ID NO: 203. 63a gene (miRBase Accession No. MIMAT0003326 ) and other species homologs or orthologs. a gene is Cummins JM et al., 2006, Proc Natl Acad S ci USA, Vol. 103, pp. 3687-3692 In addition, "hsa-miR-663a" has a hairpin-like structure as its precursor. Take "hsa-mir-663a" (miRBase Accession No. MI 0003672, SEQ ID NO: 415) is known.

[0246] As used herein, "hsa-miR-760 gene" or "hsa-miR-76 The term "miR-760" refers to the hsa-miR-760 consisting of the nucleotide sequence represented by SEQ ID NO: 204. Gene (miRBase Accession No. MIMAT0004957) and This includes homologs or orthologs of other species. Berezikov E et al., 2006, Genome Res, vol. 16, p. 128 The miR-7 gene can be obtained by the method described in JP-A-2002-1298. miR 60 has a hairpin-like structure as its precursor, hsa-mir-760 (miR Base Accession No. MI0005567, SEQ ID NO: 416) is known are.

[0247] As used herein, "hsa-miR-4667-5p gene" or "hsa-mi The term "R-4667-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 205. -miR-4667-5p gene (miRBase Accession No. MIM AT0019743) and other species homologs or orthologs. The a-miR-4667-5p gene is described in Persson H et al., 2011, Cancer It can be obtained by the method described in J. R. Res., Vol. 71, pp. 78-86. "hsa-miR-4667-5p" is a precursor of "hs a-mir-4667” (miRBase Accession No. MI00172 97, SEQ ID NO: 417) is known.

[0248] As used herein, "hsa-miR-6090 gene" or "hsa-miR-6 The term "hsa-miR-6.090" refers to the hsa-miR-6 consisting of the nucleotide sequence represented by SEQ ID NO: 206. 090 gene (miRBase Accession No. MIMAT0023715 ) and other species homologs or orthologs. 0 gene, Yoo JK et al., 2012, Stem Cells Dev, Vol. 21, p. 2049-2057. R-6090 has a hairpin-like structure as its precursor, hsa-mir-6090 ” (miRBase Accession No. MI0020367, SEQ ID NO: 418 ) is known.

[0249] As used herein, "hsa-miR-4730 gene" or "hsa-miR-4 The term "miR-4" refers to the hsa-miR-4 having the nucleotide sequence represented by SEQ ID NO: 207. 730 genes (miRBase Accession No. MIMAT0019852 ) and other species homologs or orthologs. 0 gene, Persson H et al., 2011, Cancer Res, Vol. 71, p. 7 8-86. 0” has a hairpin-like structure as its precursor, “hsa-mir-4730” (miR Base Accession No. MI0017367, SEQ ID NO: 419) is known are.

[0250] As used herein, "hsa-miR-7106-5p gene" or "hsa-mi The term "R-7106-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 208. -miR-7106-5p gene (miRBase Accession No. MIM AT0028109) and other species homologs or orthologs. The a-miR-7106-5p gene was identified in Ladewig E et al., 2012, Genom e Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-7106-5p" has a hairpin-like structure as its precursor. The miRBase Accession No. MI0 022957, SEQ ID NO: 420) is known.

[0251] As used herein, "hsa-miR-3196 gene" or "hsa-miR-3 The term "miR-3" refers to the hsa-miR-3 having the nucleotide sequence represented by SEQ ID NO: 209. 196 genes (miRBase Accession No. MIMAT0015080 ) and other species homologs or orthologs. 6 genes are listed in Stark MS et al., 2010, PLoS One, Vol. 5, e9685 hsa-miR-3196 can be obtained by the method described in The precursor of the hairpin-like structure "hsa-mir-3196" (miRBase Accession No. MI0014241, SEQ ID NO: 421) is known.

[0252] As used herein, "hsa-miR-5698 gene" or "hsa-miR-5 The term "miR-5" refers to the hsa-miR-5 gene consisting of the nucleotide sequence represented by SEQ ID NO: 210. 698 genes (miRBase Accession No. MIMAT0022491 ) and other species homologs or orthologs. 8 genes are from Watahiki A et al., 2011, PLoS One, Vol. 6, e249 50. Also, "hsa-miR-5698" can be obtained by the method described in The precursor of miRBa is a hairpin-like structure called "hsa-mir-5698" (miRBa (Accession No. MI0019305, SEQ ID NO: 422) do.

[0253] As used herein, "hsa-miR-6087 gene" or "hsa-miR-6 The term "miR-6" refers to the hsa-miR-6 consisting of the nucleotide sequence represented by SEQ ID NO: 211. 087 gene (miRBase Accession No. MIMAT0023712 ) and other species homologs or orthologs. 7 genes are Yoo JK et al., 2012, Stem Cells Dev, Vol. 21, p. 2049-2057. R-6087 has a hairpin-like structure as its precursor, hsa-mir-6087 ” (miRBase Accession No. MI0020364, SEQ ID NO: 423 ) is known.

[0254] As used herein, "hsa-miR-4665-5p gene" or "hsa-mi The term "R-4665-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 212. -miR-4665-5p gene (miRBase Accession No. MIM AT0019739) and other species homologs or orthologs. The a-miR-4665-5p gene is described in Persson H et al., 2011, Cancer It can be obtained by the method described in J. R. Res., Vol. 71, pp. 78-86. "hsa-miR-4665-5p" is a precursor of "hs a-mir-4665” (miRBase Accession No. MI00172 95, SEQ ID NO: 275) is known.

[0255] As used herein, "hsa-miR-8059 gene" or "hsa-miR-8 The term "hsa-miR-8.059" refers to the hsa-miR-8 consisting of the nucleotide sequence represented by SEQ ID NO: 213. 059 gene (miRBase Accession No. MIMAT0030986 ) and other species homologs or orthologs. 9 genes are listed in Wang HJ et al., 2013, Shock, Vol. 39, p. 480-487 hsa-miR-8059 can be obtained by the method described in The precursor of the miR gene, "hsa-mir-8059" (miRBase Accession No. MI0025895, SEQ ID NO: 424) is known.

[0256] As used herein, "hsa-miR-6879-5p gene" or "hsa-mi The term "R-6879-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 214. -miR-6879-5p gene (miRBase Accession No. MIM AT0027658) and other species homologs or orthologs. The a-miR-6879-5p gene was identified in Ladewig E et al., 2012, Genom e Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6879-5p" has a hairpin-like structure as its precursor. The miRBase Accession No. MI0 022726, SEQ ID NO: 425) is known.

[0257] As used herein, "hsa-miR-6717-5p gene" or "hsa-mi The term "R-6717-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 666. -miR-6717-5p gene (miRBase Accession No. MIM AT0025846) and other species homologs or orthologs. The sa-miR-6717-5p gene is described in Li Y et al., 2012, Gene, Vol. 497. It can be obtained by the method described in the "Hsa-mi R-6717-5p is a precursor of hsa-mir-6, which has a hairpin-like structure. 717" (miRBase Accession No. MI0022551, SEQ ID NO: 677) is known.

[0258] As used herein, "hsa-miR-3648 gene" or "hsa-miR-3 The term "hsa-miR-3" refers to the hsa-miR-3 having the nucleotide sequence represented by SEQ ID NO: 667. 648 genes (miRBase Accession No. MIMAT0018068 ) and other species homologs or orthologs. 48 genes were identified in Meiri E et al., 2010, Nucleic Acids Res. 38, pp. 6234-6246. sa-miR-3648 has a hairpin-like structure as its precursor, hsa-mir -3648" (miRBase Accession No. MI0016048, sequence Number 678) is known.

[0259] As used herein, "hsa-miR-3162-5p gene" or "hsa-mi The term "R-3162-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 668. -miR-3162-5p gene (miRBase Accession No. MIM AT0015036) and other species homologs or orthologs. The sa-miR-3162-5p gene is described in Stark MS et al., 2010, PLoS It can be obtained by the method described in J.S.A., Vol. 5, E9685. -miR-3162-5p has a hairpin-like structure as its precursor, "hsa-mi r-3162” (miRBase Accession No. MI0014192, Column number 679) is known.

[0260] As used herein, "hsa-miR-1909-3p gene" or "hsa-mi The term "R-1909-3p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 669. -miR-1909-3p gene (miRBase Accession No. MIM AT0007883) and other species homologs or orthologs. The sa-miR-1909-3p gene was identified in Bar M et al., 2008, Stem Cel It can be obtained by the method described in ls, Vol. 26, pp. 2496-2505. "hsa-miR-1909-3p" has a hairpin-like structure as its precursor, "h sa-mir-1909” (miRBase Accession No. MI0008 330, SEQ ID NO: 680) is known.

[0261] As used herein, "hsa-miR-8073 gene" or "hsa-miR-8 The term "hsa-miR-8.073" refers to the hsa-miR-8 consisting of the nucleotide sequence represented by SEQ ID NO: 670. 073 genes (miRBase Accession No. MIMAT0031000 ) and other species homologs or orthologs. 73 genes are from Wang HJ et al., 2013, Shock, Vol. 39, p480-487 Furthermore, "hsa-miR-8073" can be obtained by the method described in Its precursor, "hsa-mir-8073" (miRBase Accession No. MI0025909, SEQ ID NO: 681) is known.

[0262] As used herein, "hsa-miR-6769b-5p gene" or "hsa-m The term "iR-6769b-5p" refers to a nucleic acid sequence consisting of the nucleic acid sequence represented by SEQ ID NO: 671. sa-miR-6769b-5p gene (miRBase Accession No. MIMAT0027620) and other species homologs or orthologs. The hsa-miR-6769b-5p gene was identified in Ladewig E et al., 2012, It can be obtained by the method described in Genome Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6769b-5p" is a precursor of hairpin The miRBase Accession No. 1000012666444444 has a phosphodiesterase-like structure, "hsa-mir-6769b" (miRBase Accession No. 10000126664444444). No. MI0022706, SEQ ID NO: 682) is known.

[0263] As used herein, the term "hsa-miR-6836-3p gene" or "hsa-mi The term "R-6836-3p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 672. -miR-6836-3p gene (miRBase Accession No. MIM AT0027575) and other species homologs or orthologs. The sa-miR-6836-3p gene was identified in Ladewig E et al., 2012, Geno It can be obtained by the method described in Me Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6836-3p" has a hairpin-like structure as its precursor. Take "hsa-mir-6836" (miRBase Accession No. MI 0022682, SEQ ID NO: 683) is known.

[0264] As used herein, "hsa-miR-4484 gene" or "hsa-miR-4 The term "miR-4" refers to the hsa-miR-4 consisting of the nucleotide sequence represented by SEQ ID NO: 673. 484 genes (miRBase Accession No. MIMAT0019018 ) and other species homologs or orthologs. 84 genes are listed in Jima DD et al., 2010, Blood, Vol. 116, e118-e1 27. Also, "hsa-miR-4484" The precursor of miRBa is a hairpin-like structure called "hsa-mir-4484" (miRBa (Accession No. MI0016845, SEQ ID NO: 684) do.

[0265] As used herein, "hsa-miR-6819-5p gene" or "hsa-mi The term "R-6819-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 674. -miR-6819-5p gene (miRBase Accession No. MIM AT0027538) and other species homologs or orthologs. The sa-miR-6819-5p gene was identified in Ladewig E et al., 2012, Geno It can be obtained by the method described in Me Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6819-5p" has a hairpin-like structure as its precursor. Take "hsa-mir-6819" (miRBase Accession No. MI 0022664, SEQ ID NO: 685) is known.

[0266] As used herein, "hsa-miR-6794-5p gene" or "hsa-mi The term "R-6794-5p" refers to an hsa consisting of the nucleotide sequence represented by SEQ ID NO: 675. -miR-6794-5p gene (miRBase Accession No. MIM AT0027488) and other species homologs or orthologs. The sa-miR-6794-5p gene was identified in Ladewig E et al., 2012, Geno It can be obtained by the method described in Me Res, Vol. 22, pp. 1634-1645. In addition, "hsa-miR-6794-5p" has a hairpin-like structure as its precursor. Take "hsa-mir-6794" (miRBase Accession No. MI 0022639, SEQ ID NO: 686) is known.

[0267] As used herein, "hsa-miR-24-3p gene" or "hsa-miR- The term "hsa-miR 24-3p" refers to the hsa-miR consisting of the nucleotide sequence represented by SEQ ID NO: 676. -24-3p gene (miRBase Accession No. MIMAT0000 080) and other species homologs or orthologs. The -24-3p gene is described in Lagos-Quintana M et al., 2001, Science ce, Vol. 294, pp. 853-858. "hsa-miR-24-3p" is a precursor of "hsa- mir-24-1,hsa-mir-24-2” (miRBase Accession No. MI0000080, MI0000081, sequence numbers 687 and 688) are known are.

[0268] In addition, mature miRNAs are formed from RNA precursors with hairpin-like structures. When excised as A, one to several bases before and after the sequence may be excised shorter or longer, Base substitutions can occur, resulting in mutants, called isomiRs (Morin R D. et al., 2008, Genome Res., Vol. 18, pp. 610-621). In RBase Release 20, any of SEQ ID NOs: 1 to 214 and 666 to 676 In addition to the base sequences shown above, there are numerous isomiRs, such as those shown in SEQ ID NOs: 426 to 665. Variants and fragments of the nucleotide sequences represented by any of nucleotides 689 to 700 are also shown. These mutants also contain the bases represented by any of SEQ ID NOs: 1 to 214 and 666 to 676. It can be obtained as miRNA having the sequence.

[0269] That is, SEQ ID NOs: 1, 2, 6, 9, 13, 18, 20, 21, 23, 28, 29, 30, 31, 34, 36, 40, 41, 46, 47, 50, 52, 54, 55, 5 6, 58, 64, 66, 67, 68, 72, 73, 74, 76, 77, 79, 80, 83 , 84, 85, 87, 89, 90, 91, 92, 93, 94, 95, 97, 99, 100 , 101, 102, 104, 108, 110, 112, 113, 114, 117, 118 , 120, 121, 122, 124, 125, 126, 127, 128, 129, 130 , 132, 134, 135, 136, 137, 142, 143, 145, 146, 147 , 148, 149, 150, 151, 152, 153, 155, 156, 158, 160 , 162, 164, 166, 167, 173, 174, 178, 182, 183, 184 , 185, 186, 187, 188, 189, 190, 191, 193, 194, 196 , 199, 201, 203, 204, 205, 207, 209, 210, 211, 212 , 666, 667, 668, 669, 673 and 676, or salts thereof Among the variants of polynucleotides consisting of a base sequence in which u is replaced by t, e.g. The longest variants registered in miRBase Release 20 are Sequence numbers 426, 428, 430, 432, 434, 436, 438, 440, and 442 , 444, 446, 448, 450, 452, 454, 456, 458, 460, 462 , 464, 466, 468, 470, 472, 474, 476, 478, 480, 482 , 484, 486, 488, 490, 492, 494, 496, 498, 500, 502 , 504, 506, 508, 510, 512, 514, 516, 518, 520, 522 , 524, 526, 528, 530, 532, 534, 536, 538, 540, 542 , 544, 546, 548, 550, 552, 554, 556, 558, 560, 562 , 564, 566, 568, 570, 572, 574, 576, 578, 580, 582 , 584, 586, 588, 590, 592, 594, 596, 598, 600, 602 , 604, 606, 608, 610, 612, 614, 616, 618, 620, 622 , 624, 626, 628, 630, 632, 634, 636, 638, 640, 642 , 644, 646, 648, 650, 652, 654, 656, 658, 660, 662 , 664, 689, 691, 693, 695, 697, and 699 In addition, the present invention also includes SEQ ID NOs: 1, 2, 6, 9, 13, 18, 20, 21, 23, 28, 29, 30, 31, 34, 36, 40, 41, 46, 47, 50, 52, 5 4, 55, 56, 58, 64, 66, 67, 68, 72, 73, 74, 76, 77, 79 , 80, 83, 84, 85, 87, 89, 90, 91, 92, 93, 94, 95, 97, 99, 100, 101, 102, 104, 108, 110, 112, 113, 114, 1 17, 118, 120, 121, 122, 124, 125, 126, 127, 128, 1 29, 130, 132, 134, 135, 136, 137, 142, 143, 145, 1 46, 147, 148, 149, 150, 151, 152, 153, 155, 156, 1 58, 160, 162, 164, 166, 167, 173, 174, 178, 182, 1 83, 184, 185, 186, 187, 188, 189, 190, 191, 193, 1 94, 196, 199, 201, 203, 204, 205, 207, 209, 210, 2 Nucleotide sequences represented by 11, 212, 666, 667, 668, 669, 673 and 676 or a variant of the polynucleotide consisting of a base sequence in which u is t in the base sequence For example, the shortest variant registered in miRBase Release 20 and SEQ ID NOs: 427, 429, 431, 433, 435, 437, 439, 441, 443, 445, 447, 449, 451, 453, 455, 457, 459, 461, 463, 465, 467, 469, 471, 473, 475, 477, 479, 481, 483, 485, 487, 489, 491, 493, 495, 497, 499, 501, 503, 505, 507, 509, 511, 513, 515, 517, 519, 521, 523, 525, 527, 529, 531, 533, 535, 537, 539, 541, 543, 545, 547, 549, 551, 553, 555, 557, 559, 561, 563, 565, 567, 569, 571, 573, 575, 577, 579, 581, 583, 585, 587, 589, 591, 593, 595, 597, 599, 601, 603, 605, 607, 609, 611, 613, 615, 617, 619, 621, 623, 625, 627, 629, 631, 633, 635, 637, 639, 641, 643, 645, 647, 649, 651, 653, 655, 657, 659, Represented by 661, 663, 665, 690, 692, 694, 696, 698 and 700 In addition to these variants and fragments, polynucleotides having sequences similar to those of the present invention may also be used. The base sequences represented by SEQ ID NOs: 1 to 214 and 666 to 676 registered in RBase Further examples of isomiR polynucleotides include those of SEQ ID NOs: 1 to 5. Examples of polynucleotides containing a base sequence represented by any one of 214 and 666 to 676 are: As for the precursors, SEQ ID NOs: 215 to 425 and 677 to 688 are used. Examples include polynucleotides represented by the following:

[0270] The names of genes consisting of the base sequences represented by SEQ ID NOs: 1 to 700 and miRBase Ac The accession numbers are listed in Table 1.

[0271] As used herein, the term "capable of specifically binding" refers to the nucleic acid probe or probe used in the present invention. This means that the primer binds to a specific target nucleic acid and is substantially incapable of binding to other nucleic acids. .

[0272] [Table 1]

[0273] TIFF2025143453000002.tif245135

[0274] TIFF2025143453000003.tif244134

[0275] TIFF2025143453000004.tif245135

[0276] TIFF2025143453000005.tif245133

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[0278] TIFF2025143453000007.tif246135

[0279] TIFF2025143453000008.tif245135

[0280] TIFF2025143453000009.tif246135

[0281] TIFF2025143453000010.tif244134

[0282] TIFF2025143453000011.tif245135

[0283] TIFF2025143453000012.tif245136

[0284] TIFF2025143453000013.tif246134

[0285] TIFF2025143453000014.tif246136

[0286] TIFF2025143453000015.tif245135

[0287] TIFF2025143453000016.tif245136

[0288] TIFF2025143453000017.tif244135

[0289] TIFF2025143453000018.tif245135

[0290] TIFF2025143453000019.tif245134

[0291] This specification is a joint publication of Japanese Patent Application Nos. 2014-125036 and 2015-070379, from which the present application claims priority. It includes the contents described in the specification and / or drawings. [Effects of the Invention]

[0292] The present invention makes it possible to detect esophageal cancer easily and with high accuracy. refers to measurements of several miRNAs in patients' blood, serum, and / or plasma that can be collected minimally invasively. This can be used as a target to easily detect whether a patient has esophageal cancer. [Brief explanation of the drawings]

[0293] [Figure 1] This figure shows the relationship between hsa-miR-1343-3p, which consists of the base sequence represented by SEQ ID NO: 9 and is generated from its precursor hsa-mir-1343, which consists of the base sequence represented by SEQ ID NO: 223, and hsa-miR-1343-5p, which consists of the base sequence represented by SEQ ID NO: 138. [Figure 2]Left: The vertical axis shows the measured expression levels of hsa-miR-204-3p (SEQ ID NO: 1) in healthy individuals (100 individuals) and esophageal cancer patients (34 individuals) selected as the training sample group. The horizontal line in the figure indicates the threshold (12.3) optimized by Fisher's discriminant analysis for discriminating between the two groups. Right: The vertical axis shows the measured expression levels of hsa-miR-204-3p (SEQ ID NO: 1) in healthy individuals (50 individuals) and esophageal cancer patients (16 individuals) selected as the test sample group. The horizontal line in the figure indicates the threshold (12.3) set in the training sample group for discriminating between the two groups. [Figure 3] Left: The horizontal axis shows the measured expression levels of hsa-miR-1247-3p (SEQ ID NO: 2) and the vertical axis shows the measured expression levels of hsa-miR-6857-5p (SEQ ID NO: 4) for healthy individuals (100 individuals, circles) and esophageal cancer patients (34 individuals, triangles) selected as the training sample group. The line in the figure represents the discriminant function (0 = 2.42x + y - 21.17) that discriminated between the two groups, optimized by Fisher's discriminant analysis. Right: The horizontal axis shows the measured expression levels of hsa-miR-1247-3p (SEQ ID NO: 2) and the vertical axis shows the measured expression levels of hsa-miR-6857-5p (SEQ ID NO: 4) for healthy individuals (50 individuals, circles) and esophageal cancer patients (34 individuals, triangles) selected as the test sample group. The line in the figure indicates the threshold value (0=2.42x+y-21.17) set for the training sample group to distinguish between the two groups. [Figure 4]A: hsa-miR-204-3p (SEQ ID NO: 1), hsa-miR-6726-5p (SEQ ID NO: 5), hsa-miR-4723-5p (SEQ ID NO: 85), hsa-miR-1343-5p (SEQ ID NO: 138), hsa-miR-4739 (SEQ ID NO: 16 ...4723-5p (SEQ ID NO: 85), hsa-miR-1343-5p (SEQ ID NO: 138), hsa-miR-4739 (SEQ ID NO: 166), hsa-miR-4723-5p (SEQ ID NO: 85), hsa-miR-4723-5p (SEQ ID NO: 85), hsa-miR-4723-5p (SEQ ID NO: 85), hsa-miR-4723-5p (SEQ ID NO: 85), hsa-miR-4723- A discriminant equation was created using Fisher's discriminant analysis from the expression level measurements of R-6717-5p (SEQ ID NO: 666) (-2.65xhsa-miR-4739-3.01xhsa-miR-1343-5p + 0.69xhsa-miR-204-3p + 0.95xhsa-miR-4723-5p-0.56xhsa-miR-6726-5p-0.99xhsa-miR-6717-5p + 57.33), and the discriminant score obtained from the discriminant equation is plotted on the vertical axis and the sample group on the horizontal axis. The dotted line in the figure indicates the discriminant boundary for discriminating between both groups where the discriminant score is 0. B: The test sample group consisted of 16 esophageal cancer patients, 47 healthy individuals, 30 pancreatic cancer patients, 33 biliary tract cancer patients, 20 colorectal cancer patients, 17 gastric cancer patients, 20 liver cancer patients, and 6 patients with benign pancreatic and biliary tract diseases. The expression levels of hsa-miR-204-3p (SEQ ID NO: 1), hsa-miR-6726-5p (SEQ ID NO: 5), hsa-miR-4723-5p (SEQ ID NO: 85), hsa-miR-1343-5p (SEQ ID NO: 138), hsa-miR-4739 (SEQ ID NO: 166), and hsa-miR-6717-5p (SEQ ID NO: 666) were measured. The discriminant scores obtained from the discriminant formula created for the training sample group are plotted on the vertical axis, and the sample group is plotted on the horizontal axis. The dotted line in the figure indicates the discriminant boundary for discriminating between the two groups, where the discriminant score is 0. DETAILED DESCRIPTION OF THE INVENTION

[0294] The present invention will be described in more detail below. 1. Target nucleic acid for esophageal cancer The nucleic acid probe or primer for detecting esophageal cancer defined above according to the present invention is used to detect esophageal cancer. as an esophageal cancer marker for detecting the presence and / or absence of esophageal cancer or esophageal cancer cells; The main target nucleic acids of miR-204-3p and miR-1247-3 p, hsa-miR-6875-5p, hsa-miR-6857-5p, hsa-mi R-6726-5p, hsa-miR-3188, hsa-miR-8069, hsa- miR-4257, hsa-miR-1343-3p, hsa-miR-7108-5p , hsa-miR-6825-5p, hsa-miR-7641, hsa-miR-31 85, hsa-miR-4746-3p, hsa-miR-6791-5p, hsa-m iR-6893-5p, hsa-miR-4433b-3p, hsa-miR-3135 b, hsa-miR-6781-5p, hsa-miR-1908-5p, hsa-mi R-4792, hsa-miR-7845-5p, hsa-miR-4417, hsa- miR-3184-5p, hsa-miR-1225-5p, hsa-miR-1231 , hsa-miR-1225-3p, hsa-miR-150-3p, hsa-miR- 4433-3p, hsa-miR-6125, hsa-miR-4513, hsa-mi R-6787-5p, hsa-miR-6784-5p, hsa-miR-615-5p , hsa-miR-6765-3p, hsa-miR-5572, hsa-miR-68 42-5p, hsa-miR-8063, hsa-miR-6780b-5p, hsa- miR-187-5p, hsa-miR-128-1-5p, hsa-miR-6729 -5p, hsa-miR-6741-5p, hsa-miR-6757-5p, hsa- miR-7110-5p, hsa-miR-7975, hsa-miR-1233-5p hsa-miR-6845-5p, hsa-miR-3937, hsa-miR-44 67、hsa-miR-7109-5p、hsa-miR-6088、hsa-miR- 6782-5p、hsa-miR-5195-3p、hsa-miR-4454、hsa -miR-6724-5p、hsa-miR-8072、hsa-miR-4516、h sa-miR-6756-5p、hsa-miR-4665-3p、hsa-miR-6 826-5p、hsa-miR-6820-5p、hsa-miR-6887-5p、h sa-miR-3679-5p、hsa-miR-7847-3p、hsa-miR-6 721-5p、hsa-miR-3622a-5p、hsa-miR-939-5p、h sa-miR-602、hsa-miR-7977、hsa-miR-6749-5p、 hsa-miR-1914-3p, hsa-miR-4651, hsa-miR-469 5-5p、hsa-miR-6848-5p、hsa-miR-1228-3p、hsa -miR-642b-3p、hsa-miR-6746-5p、hsa-miR-362 0-5p、hsa-miR-3131、hsa-miR-6732-5p、hsa-mi R-7113-3p、hsa-miR-23a-3p、hsa-miR-3154、hs a-miR-4723-5p、hsa-miR-3663-3p、hsa-miR-47 34、hsa-miR-6816-5p、hsa-miR-4442、hsa-miR- 4476、hsa-miR-423-5p、hsa-miR-1249、hsa-miR -6515-3p、hsa-miR-887-3p、hsa-miR-4741、hsa <h2 style=";text-align:left;direction:ltr">-miR-6766-3p、hsa-miR-4673、hsa-miR-6779-5<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> p、hsa-miR-4706、hsa-miR-1268b、hsa-miR-463<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> 2-5p、hsa-miR-3197、hsa-miR-6798-5p、hsa-mi<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> R-711、hsa-miR-6840-3p、hsa-miR-6763-5p、hs<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> a-miR-6727-5p、hsa-miR-371a-5p、hsa-miR-68<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> 24-5p、hsa-miR-4648、hsa-miR-1227-5p、hsa-m<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> iR-564、hsa-miR-3679-3p、hsa-miR-2861、hsa-<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> miR-6737-5p、hsa-miR-4725-3p、hsa-miR-6716<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> -5p、hsa-miR-4675、hsa-miR-1915-3p、hsa-miR<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> -671-5p、hsa-miR-3656、hsa-miR-6722-3p、hsa<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> -miR-4707-5p、hsa-miR-4449、hsa-miR-1202、h<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> sa-miR-4649-5p、hsa-miR-744-5p、hsa-miR-64<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> 2a-3p、hsa-miR-451a、hsa-miR-6870-5p、hsa-m<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> iR-4443、hsa-miR-6808-5p、hsa-miR-4728-5p、<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> hsa-miR-937-5p、hsa-miR-135a-3p、hsa-miR-6<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> 63b、hsa-miR-1343-5p、hsa-miR-6822-5p、hsa-<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> miR-6803-5p、hsa-miR-6805-3p、hsa-miR-128-<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> 2-5p、hsa-miR-4640-5p、hsa-miR-1469、hsa-mi<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> R-92a-2-5p、hsa-miR-3940-5p、hsa-miR-4281、 hsa-miR-1260b、hsa-miR-4758-5p、hsa-miR-19 15-5p、hsa-miR-5001-5p、hsa-miR-4286、hsa-m iR-6126、hsa-miR-6789-5p、hsa-miR-4459、hsa -miR-1268a、hsa-miR-6752-5p、hsa-miR-6131、 hsa-miR-6800-5p、hsa-miR-4532、hsa-miR-687 2-3p、hsa-miR-718、hsa-miR-6769a-5p、hsa-mi R-4707-3p、hsa-miR-6765-5p、hsa-miR-4739、h sa-miR-4525、hsa-miR-4270、hsa-miR-4534、hs a-miR-6785-5p、hsa-miR-6850-5p、hsa-miR-46 97-5p、hsa-miR-1260a、hsa-miR-4486、hsa-miR -6880-5p、hsa-miR-6802-5p、hsa-miR-6861-5p 、hsa-miR-92b-5p、hsa-miR-1238-5p、hsa-miR- 6851-5p、hsa-miR-7704、hsa-miR-149-3p、hsa- miR-4689、hsa-miR-4688、hsa-miR-125a-3p、hs a-miR-23b-3p、hsa-miR-614、hsa-miR-1913、hs a-miR-16-5p、hsa-miR-6717-5p、hsa-miR-3648 hsa-miR-3162-5p, hsa-miR-1909-3p, hsa-miR -8073、hsa-miR-6769b-5p、hsa-miR-6836-3p、h sa-miR-4484, hsa-miR-6819-5p, and hsa-miR-67 At least one miRNA selected from the group consisting of 94-5p can be used. Furthermore, other esophageal cancer markers that can be combined with these miRNAs, namely The miRNAs hsa-miR-575 and hsa-miR-24-3p are also targets. It can be preferably used as a nucleic acid. Other esophageal cancer markers that can be used include hsa-miR-675-5p, hsa-m iR-486-3p, hsa-miR-6777-5p, hsa-miR-4497, h sa-miR-296-3p, hsa-miR-6738-5p, hsa-miR-47 31-5p, hsa-miR-6889-5p, hsa-miR-6786-5p, hs a-miR-92a-3p, hsa-miR-4294, hsa-miR-4763-3 p, hsa-miR-6076, hsa-miR-663a, hsa-miR-760, hsa-miR-4667-5p, hsa-miR-6090, hsa-miR-473 0, hsa-miR-7106-5p, hsa-miR-3196, hsa-miR-5 698, hsa-miR-6087, hsa-miR-4665-5p, hsa-miR at least one selected from the group consisting of miR-6879-5p and miR-8059; miRNAs can also be preferably used as target nucleic acids.

[0295] The miRNAs include, for example, any of SEQ ID NOs: 1 to 214 and 666 to 676. Human genes containing the base sequences represented by the miR-204-3 p、hsa-miR-1247-3p、hsa-miR-6875-5p、hsa-mi R-6857-5p、hsa-miR-6726-5p、hsa-miR-3188、h sa-miR-8069、hsa-miR-4257、hsa-miR-1343-3p hsa-miR-7108-5p, hsa-miR-6825-5p, hsa-miR -7641、hsa-miR-3185、hsa-miR-4746-3p、hsa-m iR-6791-5p、hsa-miR-6893-5p、hsa-miR-4433b -3p、hsa-miR-3135b、hsa-miR-6781-5p、hsa-mi R-1908-5p、hsa-miR-4792、hsa-miR-7845-5p、h sa-miR-4417, hsa-miR-3184-5p, hsa-miR-1225 -5p、hsa-miR-1231、hsa-miR-1225-3p、hsa-miR -150-3p、hsa-miR-4433-3p、hsa-miR-6125、hsa -miR-4513、hsa-miR-6787-5p、hsa-miR-6784-5 p、hsa-miR-615-5p、hsa-miR-6765-3p、hsa-miR -5572、hsa-miR-6842-5p、hsa-miR-8063、hsa-m iR-6780b-5p、hsa-miR-187-5p、hsa-miR-128-1 -5p、hsa-miR-6729-5p、hsa-miR-6741-5p、hsa- miR-6757-5p、hsa-miR-7110-5p、hsa-miR-7975 hsa-miR-1233-5p, hsa-miR-6845-5p, hsa-miR -3937、hsa-miR-4467、hsa-miR-7109-5p、hsa-m iR-6088、hsa-miR-6782-5p、hsa-miR-5195-3p、 hsa-miR-4454, hsa-miR-6724-5p, hsa-miR-807 2, hsa-miR-4516, hsa-miR-6756-5p, hsa-miR-4 665-3p、hsa-miR-6826-5p、hsa-miR-6820-5p、h sa-miR-6887-5p、hsa-miR-3679-5p、hsa-miR-7 847-3p、hsa-miR-6721-5p、hsa-miR-3622a-5p、 hsa-miR-939-5p、hsa-miR-602、hsa-miR-7977、 hsa-miR-6749-5p、hsa-miR-1914-3p、hsa-miR- 4651、hsa-miR-4695-5p、hsa-miR-6848-5p、hsa -miR-1228-3p、hsa-miR-642b-3p、hsa-miR-674 6-5p、hsa-miR-3620-5p、hsa-miR-3131、hsa-mi R-6732-5p、hsa-miR-7113-3p、hsa-miR-23a-3p hsa-miR-3154, hsa-miR-4723-5p, hsa-miR-36 63-3p、hsa-miR-4734、hsa-miR-6816-5p、hsa-m iR-4442、hsa-miR-4476、hsa-miR-423-5p、hsa- miR-1249, hsa-miR-6515-3p, hsa-miR-887-3p hsa-miR-4741, hsa-miR-6766-3p, hsa-miR-467 3, hsa-miR-6779-5p, hsa-miR-4706, hsa-miR-1 268b、hsa-miR-4632-5p、hsa-miR-3197、hsa-mi R-6798-5p、hsa-miR-711、hsa-miR-6840-3p、hs a-miR-6763-5p、hsa-miR-6727-5p、hsa-miR-37 1a-5p、hsa-miR-6824-5p、hsa-miR-4648、hsa-m iR-1227-5p、hsa-miR-564、hsa-miR-3679-3p、h sa-miR-2861、hsa-miR-6737-5p、hsa-miR-4725 -3p、hsa-miR-6716-5p、hsa-miR-4675、hsa-miR -1915-3p、hsa-miR-671-5p、hsa-miR-3656、hsa -miR-6722-3p、hsa-miR-4707-5p、hsa-miR-444 9, hsa-miR-1202, hsa-miR-4649-5p, hsa-miR-7 44-5p、hsa-miR-642a-3p、hsa-miR-451a、hsa-m iR-6870-5p、hsa-miR-4443、hsa-miR-6808-5p、 hsa-miR-4728-5p、hsa-miR-937-5p、hsa-miR-1 35a-3p、hsa-miR-663b、hsa-miR-1343-5p、hsa- miR-6822-5p、hsa-miR-6803-5p、hsa-miR-6805 -3p、hsa-miR-128-2-5p、hsa-miR-4640-5p、hsa -miR-1469、hsa-miR-92a-2-5p、hsa-miR-3940- 5p、hsa-miR-4281、hsa-miR-1260b、hsa-miR-47 58-5p、hsa-miR-1915-5p、hsa-miR-5001-5p、hs a-miR-4286、hsa-miR-6126、hsa-miR-6789-5p、 hsa-miR-4459、hsa-miR-1268a、hsa-miR-6752- 5p、hsa-miR-6131、hsa-miR-6800-5p、hsa-miR- 4532、hsa-miR-6872-3p、hsa-miR-718、hsa-miR -6769a-5p、hsa-miR-4707-3p、hsa-miR-6765-5 p、hsa-miR-4739、hsa-miR-4525、hsa-miR-4270 hsa-miR-4534, hsa-miR-6785-5p, hsa-miR-68 50-5p、hsa-miR-4697-5p、hsa-miR-1260a、hsa- miR-4486, hsa-miR-6880-5p, hsa-miR-6802-5p 、hsa-miR-6861-5p、hsa-miR-92b-5p、hsa-miR- 1238-5p、hsa-miR-6851-5p、hsa-miR-7704、hsa -miR-149-3p、hsa-miR-4689、hsa-miR-4688、hs a-miR-125a-3p、hsa-miR-23b-3p、hsa-miR-614 hsa-miR-1913, hsa-miR-16-5p, hsa-miR-6717 -5p、hsa-miR-3648、hsa-miR-3162-5p、hsa-miR -1909-3p、hsa-miR-8073、hsa-miR-6769b-5p、h sa-miR-6836-3p、hsa-miR-4484、hsa-miR-6819 -5p、hsa-miR-6794-5phsa-miR-575、hsa-miR-2 4-3p, hsa-miR-675-5p, hsa-miR-486-3p, hsa-m iR-6777-5p, hsa-miR-4497, hsa-miR-296-3p, h sa-miR-6738-5p, hsa-miR-4731-5p, hsa-miR-6 889-5p, hsa-miR-6786-5p, hsa-miR-92a-3p, hs a-miR-4294, hsa-miR-4763-3p, hsa-miR-6076, hsa-miR-663a, hsa-miR-760, hsa-miR-4667-5p , hsa-miR-6090, hsa-miR-4730, hsa-miR-7106- 5p, hsa-miR-3196, hsa-miR-5698, hsa-miR-608 7, hsa-miR-4665-5p, hsa-miR-8059 and hsa-miR- 6879-5p), its homologs, its transcription products, and its variants or derivatives. Here, the terms gene, homologue, transcription product, variant and derivative are as defined above.

[0296] A preferred target nucleic acid is a human gene comprising a base sequence represented by any one of SEQ ID NOs: 1 to 700. A gene or its transcription product, more preferably, the transcription product, i.e., miRNA, The miRNA is a precursor RNA of the miRNA, pri-miRNA or pre-miRNA.

[0297] The first target gene is the hsa-miR-204-3p gene, its homologs, and their The gene or its transcription product, or its variant or derivative. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0298] The second target gene is the hsa-miR-1247-3p gene, its homologs, and The gene is a transcription product of the gene, or a variant or derivative thereof. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0299] The third target gene is the hsa-miR-6875-5p gene, its homologs, and The gene is a transcription product of the gene, or a variant or derivative thereof. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0300] The fourth target gene is the hsa-miR-6857-5p gene, its homologs, and The gene is a transcription product of the gene, or a variant or derivative thereof. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0301] The fifth target gene is the hsa-miR-6726-5p gene, its homologs, and The gene is a transcription product of the gene, or a variant or derivative thereof. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0302] The sixth target gene is the hsa-miR-3188 gene, its homologs, and their transcription factors. The gene or its transcript, or a variant or derivative thereof, has not been There are no known reports that altered expression of these proteins could be markers for esophageal cancer.

[0303] The seventh target gene is the hsa-miR-8069 gene, its homologs, and their transcription factors. The gene or its transcript, or a variant or derivative thereof, has not been There are no known reports that altered expression of these proteins could be markers for esophageal cancer.

[0304] The eighth target gene is the hsa-miR-4257 gene, its homologs, and their transcription factors. The gene or its transcript, or a variant or derivative thereof, has not been There are no known reports that altered expression of these proteins could be markers for esophageal cancer.

[0305] The ninth target gene is the hsa-miR-1343-3p gene, its homologs, and The gene is a transcription product of the gene, or a variant or derivative thereof. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0306] The tenth target gene is the hsa-miR-7108-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0307] The eleventh target gene is the hsa-miR-6825-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0308] The 12th target gene is the hsa-miR-7641 gene, its homologs, and their The gene or its transcription product, or a variant or derivative thereof. There are no known reports suggesting that changes in expression of these products could be markers for esophageal cancer.

[0309] The 13th target gene is the hsa-miR-3185 gene, its homologs, and their The gene or its transcription product, or a variant or derivative thereof. There are no known reports suggesting that changes in expression of these products could be markers for esophageal cancer.

[0310] The 14th target gene is the hsa-miR-4746-3p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0311] The 15th target gene is the hsa-miR-6791-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0312] The 16th target gene is the hsa-miR-6893-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0313] The 17th target gene is the hsa-miR-4433b-3p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0314] The 18th target gene is the hsa-miR-3135b gene, its homologs, and their The gene or its transcription product, or its variant or derivative. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0315] The 19th target gene is the hsa-miR-6781-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0316] The 20th target gene is the hsa-miR-1908-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0317] The 21st target gene is the hsa-miR-4792 gene, its homologs, and their The gene or its transcription product, or a variant or derivative thereof. There are no known reports suggesting that changes in expression of these products could be markers for esophageal cancer.

[0318] The 22nd target gene is the hsa-miR-7845-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0319] The 23rd target gene is the hsa-miR-4417 gene, its homologs, and their The gene or its transcription product, or a variant or derivative thereof. There are no known reports suggesting that changes in expression of these products could be markers for esophageal cancer.

[0320] The 24th target gene is the hsa-miR-3184-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0321] The 25th target gene is the hsa-miR-1225-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0322] The 26th target gene is the hsa-miR-1231 gene, its homologs, and their The gene or its transcription product, or a variant or derivative thereof. There are no known reports suggesting that changes in expression of these products could be markers for esophageal cancer.

[0323] The 27th target gene is the hsa-miR-1225-3p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0324] The 28th target gene is the hsa-miR-150-3p gene, its homologs, and The gene is a transcription product of the gene, or a variant or derivative thereof. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0325] The 29th target gene is the hsa-miR-4433-3p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0326] The 30th target gene is the hsa-miR-6125 gene, its homologs, and their The gene or its transcription product, or a variant or derivative thereof. There are no known reports suggesting that changes in expression of these products could be markers for esophageal cancer.

[0327] The 31st target gene is the hsa-miR-4513 gene, its homologs, and their The gene or its transcription product, or a variant or derivative thereof. There are no known reports suggesting that changes in expression of these products could be markers for esophageal cancer.

[0328] The 32nd target gene is the hsa-miR-6787-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0329] The 33rd target gene is the hsa-miR-6784-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0330] The 34th target gene is the hsa-miR-615-5p gene, its homologs, and The gene is a transcription product of the gene, or a variant or derivative thereof. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0331] The 35th target gene is the hsa-miR-6765-3p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0332] The 36th target gene is the hsa-miR-5572 gene, its homologs, and their The gene or its transcription product, or a variant or derivative thereof. There are no known reports suggesting that changes in expression of these products could be markers for esophageal cancer.

[0333] The 37th target gene is the hsa-miR-6842-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0334] The 38th target gene is the hsa-miR-8063 gene, its homologs, and their The gene or its transcription product, or a variant or derivative thereof. There are no known reports suggesting that changes in expression of these products could be markers for esophageal cancer.

[0335] The 39th target gene is the hsa-miR-6780b-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0336] The 40th target gene is the hsa-miR-187-5p gene, its homologs, and The gene is a transcription product of the gene, or a variant or derivative thereof. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0337] The 41st target gene is the hsa-miR-128-1-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0338] The 42nd target gene is the hsa-miR-6729-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0339] The 43rd target gene is the hsa-miR-6741-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0340] The 44th target gene is the hsa-miR-6757-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0341] The 45th target gene is the hsa-miR-7110-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0342] The 46th target gene is the hsa-miR-7975 gene, its homologs, and their The gene or its transcription product, or a variant or derivative thereof. There are no known reports suggesting that changes in expression of these products could be markers for esophageal cancer.

[0343] The 47th target gene is the hsa-miR-1233-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0344] The 48th target gene is the hsa-miR-6845-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0345] The 49th target gene is the hsa-miR-3937 gene, its homologs, and their The gene or its transcription product, or a variant or derivative thereof. There are no known reports suggesting that changes in expression of these products could be markers for esophageal cancer.

[0346] The 50th target gene is the hsa-miR-4467 gene, its homologs, and their The gene or its transcription product, or a variant or derivative thereof. There are no known reports suggesting that changes in expression of these products could be markers for esophageal cancer.

[0347] The 51st target gene is the hsa-miR-7109-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0348] The 52nd target gene is the hsa-miR-6088 gene, its homologs, and their The gene or its transcription product, or a variant or derivative thereof. There are no known reports suggesting that changes in expression of these products could be markers for esophageal cancer.

[0349] The 53rd target gene is the hsa-miR-6782-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0350] The 54th target gene is the hsa-miR-5195-3p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0351] The 55th target gene is the hsa-miR-4454 gene, its homologs, and their The gene or its transcription product, or a variant or derivative thereof. There are no known reports suggesting that changes in expression of these products could be markers for esophageal cancer.

[0352] The 56th target gene is the hsa-miR-6724-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0353] The 57th target gene is the hsa-miR-8072 gene, its homologs, and their The gene or its transcription product, or a variant or derivative thereof. There are no known reports suggesting that changes in expression of these products could be markers for esophageal cancer.

[0354] The 58th target gene is the hsa-miR-4516 gene, its homologs, and their The gene or its transcription product, or a variant or derivative thereof. There are no known reports suggesting that changes in expression of these products could be markers for esophageal cancer.

[0355] The 59th target gene is the hsa-miR-6756-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0356] The 60th target gene is the hsa-miR-4665-3p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0357] The 61st target gene is the hsa-miR-6826-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0358] The 62nd target gene is the hsa-miR-6820-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0359] The 63rd target gene is the hsa-miR-6887-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0360] The 64th target gene is the hsa-miR-3679-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0361] The 65th target gene is the hsa-miR-7847-3p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0362] The 66th target gene is the hsa-miR-6721-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0363] The 67th target gene is the hsa-miR-3622a-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0364] The 68th target gene is the hsa-miR-939-5p gene, its homologs, and The gene is a transcription product of the gene, or a variant or derivative thereof. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0365] The 69th target gene is the hsa-miR-602 gene, its homologs, and their transcription factors. The gene or its transcript, or a variant or derivative thereof, has not been There are no known reports that altered expression of these proteins could be markers for esophageal cancer.

[0366] The 70th target gene is the hsa-miR-7977 gene, its homologs, and their The gene or its transcription product, or a variant or derivative thereof. There are no known reports suggesting that changes in expression of these products could be markers for esophageal cancer.

[0367] The 71st target gene is the hsa-miR-6749-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0368] The 72nd target gene is the hsa-miR-1914-3p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0369] The 73rd target gene is the hsa-miR-4651 gene, its homologs, and their The gene or its transcription product, or a variant or derivative thereof. There are no known reports suggesting that changes in expression of these products could be markers for esophageal cancer.

[0370] The 74th target gene is the hsa-miR-4695-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0371] The 75th target gene is the hsa-miR-6848-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0372] The 76th target gene is the hsa-miR-1228-3p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0373] The 77th target gene is the hsa-miR-642b-3p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0374] The 78th target gene is the hsa-miR-6746-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0375] The 79th target gene is the hsa-miR-3620-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0376] The 80th target gene is the hsa-miR-3131 gene, its homologs, and their The gene or its transcription product, or a variant or derivative thereof. There are no known reports suggesting that changes in expression of these products could be markers for esophageal cancer.

[0377] The 81st target gene is the hsa-miR-6732-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0378] The 82nd target gene is the hsa-miR-7113-3p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0379] The 83rd target gene is the hsa-miR-23a-3p gene, its homologs, and The gene is a transcription product of the gene, or a variant or derivative thereof. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0380] The 84th target gene is the hsa-miR-3154 gene, its homologs, and their The gene or its transcription product, or a variant or derivative thereof. There are no known reports suggesting that changes in expression of these products could be markers for esophageal cancer.

[0381] The 85th target gene is the hsa-miR-4723-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0382] The 86th target gene is the hsa-miR-3663-3p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0383] The 87th target gene is the hsa-miR-4734 gene, its homologs, and their The gene or its transcription product, or a variant or derivative thereof. There are no known reports suggesting that changes in expression of these products could be markers for esophageal cancer.

[0384] The 88th target gene is the hsa-miR-6816-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0385] The 89th target gene is the hsa-miR-4442 gene, its homologs, and their The gene or its transcript, or a variant or derivative thereof. There are no known reports that altered expression of these proteins could be markers for esophageal cancer.

[0386] The 90th target gene is the hsa-miR-4476 gene, its homologs, and their The gene or its transcription product, or a variant or derivative thereof. There are no known reports suggesting that changes in expression of these products could be markers for esophageal cancer.

[0387] The 91st target gene is the hsa-miR-423-5p gene, its homologs, and The gene is a transcription product of the gene, or a variant or derivative thereof. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0388] The 92nd target gene is the hsa-miR-1249 gene, its homologs, and their The gene or its transcription product, or a variant or derivative thereof. There are no known reports suggesting that changes in expression of these products could be markers for esophageal cancer.

[0389] The 93rd target gene is the hsa-miR-6515-3p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0390] The 94th target gene is the hsa-miR-887-3p gene, its homologs, and The gene is a transcription product of the gene, or a variant or derivative thereof. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0391] The 95th target gene is the hsa-miR-4741 gene, its homologs, and their The gene or its transcription product, or a variant or derivative thereof. There are no known reports suggesting that changes in expression of these products could be markers for esophageal cancer.

[0392] The 96th target gene is the hsa-miR-6766-3p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0393] The 97th target gene is the hsa-miR-4673 gene, its homologs, and their The gene or its transcription product, or a variant or derivative thereof. There are no known reports suggesting that changes in expression of these products could be markers for esophageal cancer.

[0394] The 98th target gene is the hsa-miR-6779-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0395] The 99th target gene is the hsa-miR-4706 gene, its homologs, and their The gene or its transcription product, or a variant or derivative thereof. There are no known reports suggesting that changes in expression of these products could be markers for esophageal cancer.

[0396] The 100th target gene is the hsa-miR-1268b gene, its homologs, and The gene is a transcription product of the gene, or a variant or derivative thereof. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0397] The 101st target gene is the hsa-miR-4632-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0398] The 102nd target gene is the hsa-miR-3197 gene, its homologs, and their The gene or its transcription product, or its variant or derivative. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0399] The 103rd target gene is the hsa-miR-6798-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0400] The 104th target gene is the hsa-miR-711 gene, its homologs, and their The gene or its transcription product, or a variant or derivative thereof. There are no known reports suggesting that changes in expression of these products could be markers for esophageal cancer.

[0401] The 105th target gene is the hsa-miR-6840-3p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0402] The 106th target gene is the hsa-miR-6763-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0403] The 107th target gene is the hsa-miR-6727-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0404] The 108th target gene is the hsa-miR-371a-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0405] The 109th target gene is the hsa-miR-6824-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0406] The 110th target gene is the hsa-miR-4648 gene, its homologs, and their The gene or its transcription product, or its variant or derivative. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0407] The 111th target gene is the hsa-miR-1227-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0408] The 112th target gene is the hsa-miR-564 gene, its homologs, and their The gene or its transcription product, or a variant or derivative thereof. There are no known reports suggesting that changes in expression of these products could be markers for esophageal cancer.

[0409] The 113th target gene is the hsa-miR-3679-3p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0410] The 114th target gene is the hsa-miR-2861 gene, its homologs, and their The gene or its transcription product, or its variant or derivative. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0411] The 115th target gene is the hsa-miR-6737-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0412] The 116th target gene is the hsa-miR-575 gene, its homologs, and their The gene or its transcription product, or a variant or derivative thereof. It has been reported that changes in the expression of these products can be markers for esophageal cancer (Patent Document 1).

[0413] The 117th target gene is the hsa-miR-4725-3p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0414] The 118th target gene is the hsa-miR-6716-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0415] The 119th target gene is the hsa-miR-4675 gene, its homologs, and their The gene or its transcription product, or its variant or derivative. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0416] The 120th target gene is the hsa-miR-1915-3p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0417] The 121st target gene is the hsa-miR-671-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0418] The 122nd target gene is the hsa-miR-3656 gene, its homologs, and their The gene or its transcription product, or its variant or derivative. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0419] The 123rd target gene is the hsa-miR-6722-3p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0420] The 124th target gene is the hsa-miR-4707-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0421] The 125th target gene is the hsa-miR-4449 gene, its homologs, and their The gene or its transcription product, or its variant or derivative. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0422] The 126th target gene is the hsa-miR-1202 gene, its homologs, and their The gene or its transcription product, or its variant or derivative. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0423] The 127th target gene is the hsa-miR-4649-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0424] The 128th target gene is the hsa-miR-744-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0425] The 129th target gene is the hsa-miR-642a-3p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0426] The 130th target gene is the hsa-miR-451a gene, its homologs, and their The gene or its transcription product, or its variant or derivative. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0427] The 131st target gene is the hsa-miR-6870-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0428] The 132nd target gene is the hsa-miR-4443 gene, its homologs, and their The gene or its transcription product, or its variant or derivative. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0429] The 133rd target gene is the hsa-miR-6808-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0430] The 134th target gene is the hsa-miR-4728-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0431] The 135th target gene is the hsa-miR-937-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0432] The 136th target gene is the hsa-miR-135a-3p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0433] The 137th target gene is the hsa-miR-663b gene, its homologs, and their The gene or its transcription product, or its variant or derivative. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0434] The 138th target gene is the hsa-miR-1343-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0435] The 139th target gene is the hsa-miR-6822-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0436] The 140th target gene is the hsa-miR-6803-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0437] The 141st target gene is the hsa-miR-6805-3p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0438] The 142nd target gene is the hsa-miR-128-2-5p gene and its homologs , their transcription products, or their mutants or derivatives. However, there are no known reports that altered expression of its transcripts can be a marker for esophageal cancer. stomach.

[0439] The 143rd target gene is the hsa-miR-4640-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0440] The 144th target gene is the hsa-miR-1469 gene, its homologs, and their The gene or its transcription product, or its variant or derivative. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0441] The 145th target gene is the hsa-miR-92a-2-5p gene and its homologs , their transcription products, or their mutants or derivatives. However, there are no known reports that altered expression of its transcripts can be a marker for esophageal cancer. stomach.

[0442] The 146th target gene is the hsa-miR-3940-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0443] The 147th target gene is the hsa-miR-4281 gene, its homologs, and their The gene or its transcription product, or its variant or derivative. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0444] The 148th target gene is the hsa-miR-1260b gene, its homologs, and The gene is a transcription product of the gene, or a variant or derivative thereof. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0445] The 149th target gene is the hsa-miR-4758-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0446] The 150th target gene is the hsa-miR-1915-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0447] The 151st target gene is the hsa-miR-5001-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0448] The 152nd target gene is the hsa-miR-4286 gene, its homologs, and their The gene or its transcription product, or its variant or derivative. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0449] The 153rd target gene is the hsa-miR-6126 gene, its homologs, and their The gene or its transcription product, or its variant or derivative. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0450] The 154th target gene is the hsa-miR-6789-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0451] The 155th target gene is the hsa-miR-4459 gene, its homologs, and their The gene or its transcription product, or its variant or derivative. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0452] The 156th target gene is the hsa-miR-1268a gene, its homologs, and The gene is a transcription product of the gene, or a variant or derivative thereof. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0453] The 157th target gene is the hsa-miR-6752-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0454] The 158th target gene is the hsa-miR-6131 gene, its homologs, and their The gene or its transcription product, or its variant or derivative. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0455] The 159th target gene is the hsa-miR-6800-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0456] The 160th target gene is the hsa-miR-4532 gene, its homologs, and their The gene or its transcription product, or its variant or derivative. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0457] The 161st target gene is the hsa-miR-6872-3p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0458] The 162nd target gene is the hsa-miR-718 gene, its homologs, and their The gene or its transcription product, or a variant or derivative thereof. There are no known reports suggesting that changes in expression of these products could be markers for esophageal cancer.

[0459] The 163rd target gene is the hsa-miR-6769a-5p gene and its homologs , their transcription products, or their mutants or derivatives. However, there are no known reports that altered expression of its transcripts can be a marker for esophageal cancer. stomach.

[0460] The 164th target gene is the hsa-miR-4707-3p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0461] The 165th target gene is the hsa-miR-6765-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0462] The 166th target gene is the hsa-miR-4739 gene, its homologs, and their The gene or its transcription product, or its variant or derivative. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0463] The 167th target gene is the hsa-miR-4525 gene, its homologs, and their The gene or its transcription product, or its variant or derivative. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0464] The 168th target gene is the hsa-miR-4270 gene, its homologs, and their The gene or its transcription product, or its variant or derivative. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0465] The 169th target gene is the hsa-miR-4534 gene, its homologs, and their The gene or its transcription product, or its variant or derivative. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0466] The 170th target gene is the hsa-miR-6785-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0467] The 171st target gene is the hsa-miR-6850-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0468] The 172nd target gene is the hsa-miR-4697-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0469] The 173rd target gene is the hsa-miR-1260a gene, its homologs, and The gene is a transcription product of the gene, or a variant or derivative thereof. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0470] The 174th target gene is the hsa-miR-4486 gene, its homologs, and their The gene or its transcription product, or its variant or derivative. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0471] The 175th target gene is the hsa-miR-6880-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0472] The 176th target gene is the hsa-miR-6802-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0473] The 177th target gene is the hsa-miR-6861-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0474] The 178th target gene is the hsa-miR-92b-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0475] The 179th target gene is the hsa-miR-1238-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0476] The 180th target gene is the hsa-miR-6851-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0477] The 181st target gene is the hsa-miR-7704 gene, its homologs, and their The gene or its transcription product, or its variant or derivative. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0478] The 182nd target gene is the hsa-miR-149-3p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0479] The 183rd target gene is the hsa-miR-4689 gene, its homologs, and their The gene or its transcription product, or its variant or derivative. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0480] The 184th target gene is the hsa-miR-4688 gene, its homologs, and their The gene or its transcription product, or its variant or derivative. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0481] The 185th target gene is the hsa-miR-125a-3p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0482] The 186th target gene is the hsa-miR-23b-3p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer.

[0483] The 187th target gene is the hsa-miR-614 gene, its homologs, and their The gene or its transcription product, or a variant or derivative thereof. There are no known reports that altered expression of these products could be a marker for esophageal cancer.

[0484] The 188th target gene is the hsa-miR-1913 gene, its homologs, and their The gene or its transcription product, or its variant or derivative. There are no known reports that altered transcript expression could be a marker for esophageal cancer.

[0485] The 189th target gene is the hsa-miR-16-5p gene, its homologs, and The gene is a transcription product of the gene, or a variant or derivative thereof. There are no known reports that altered transcript expression could be a marker for esophageal cancer.

[0486] The 190th target gene is the hsa-miR-675-5p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0487] The 191st target gene is the hsa-miR-486-3p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0488] The 192nd target gene is the hsa-miR-6777-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0489] The 193rd target gene is the hsa-miR-4497 gene, its homologs, and their The gene or its transcription product, or its variant or derivative. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0490] The 194th target gene is the hsa-miR-296-3p gene, its homologs, and These are transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0491] The 195th target gene is the hsa-miR-6738-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0492] The 196th target gene is the hsa-miR-4731-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0493] The 197th target gene is the hsa-miR-6889-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0494] The 198th target gene is the hsa-miR-6786-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0495] The 199th target gene is the hsa-miR-92a-3p gene, its homologs, and These are transcription products, or their variants or derivatives. It has been reported that changes in the expression of transcriptional products of esophageal cancer can be a marker (particularly Permitted document 1).

[0496] The 200th target gene is the hsa-miR-4294 gene, its homologs, and their The gene or its transcription product, or its variant or derivative. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0497] The 201st target gene is the hsa-miR-4763-3p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0498] The 202nd target gene is the hsa-miR-6076 gene, its homologs, and their The gene or its transcription product, or its variant or derivative. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0499] The 203rd target gene is the hsa-miR-663a gene, its homologs, and their The gene or its transcription product, or its variant or derivative. It has been reported that changes in the expression of transcripts can be a marker for esophageal cancer (Patent Document 1).

[0500] The 204th target gene is the hsa-miR-760 gene, its homologs, and their The gene or its transcription product, or a variant or derivative thereof. There are no known reports suggesting that changes in expression of these products could be markers for esophageal cancer.

[0501] The 205th target gene is the hsa-miR-4667-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0502] The 206th target gene is the hsa-miR-6090 gene, its homologs, and their The gene or its transcription product, or its variant or derivative. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0503] The 207th target gene is the hsa-miR-4730 gene, its homologs, and their The gene or its transcription product, or its variant or derivative. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0504] The 208th target gene is the hsa-miR-7106-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0505] The 209th target gene is the hsa-miR-3196 gene, its homologs, and their The gene or its transcription product, or its variant or derivative. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0506] The 210th target gene is the hsa-miR-5698 gene, its homologs, and their The gene or its transcription product, or its variant or derivative. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0507] The 211th target gene is the hsa-miR-6087 gene, its homologs, and their The gene or its transcription product, or its variant or derivative. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0508] The 212th target gene is the hsa-miR-4665-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0509] The 213th target gene is the hsa-miR-8059 gene, its homologs, and their The gene or its transcription product, or its variant or derivative. There are no known reports that altered transcript expression can be a marker for esophageal cancer.

[0510] The 214th target gene is the hsa-miR-6879-5p gene, its homologs, The gene is a transcription product thereof, or a variant or derivative thereof. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0511] The 215th target gene is the hsa-miR-6717-5p gene, its homologs, The genes or their transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0512] The 216th target gene is the hsa-miR-3648 gene, its homologs, and their or their variants or derivatives. There are no known reports suggesting that changes in expression of these products could be markers for esophageal cancer.

[0513] The 217th target gene is the hsa-miR-3162-5p gene, its homologs, The genes or their transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0514] The 218th target gene is the hsa-miR-1909-3p gene, its homologs, The genes or their transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0515] The 219th target gene is the hsa-miR-8073 gene, its homologs, and their or their variants or derivatives. There are no known reports suggesting that changes in expression of these products could be markers for esophageal cancer.

[0516] The 220th target gene is the hsa-miR-6769b-5p gene and its homologs. , their transcription products, or their variants or derivatives. There are no known reports that altered expression of this transcript could be a marker for esophageal cancer. .

[0517] The 221st target gene is the hsa-miR-6836-3p gene, its homologs, The genes or their transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0518] The 222nd target gene is the hsa-miR-4484 gene, its homologs, and their or their variants or derivatives. There are no known reports suggesting that changes in expression of these products could be markers for esophageal cancer.

[0519] The 223rd target gene is the hsa-miR-6819-5p gene, its homologs, The genes or their transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0520] The 224th target gene is the hsa-miR-6794-5p gene, its homologs, The genes or their transcription products, or their variants or derivatives. There are no known reports suggesting that altered expression of this transcript could be a marker for esophageal cancer.

[0521] The 225th target gene is the hsa-miR-24-3p gene, its homologs, and These are transcription products of the genes, or their variants or derivatives. It has been reported that changes in the expression of transcripts can be a marker for esophageal cancer (Patent Document 1). 2. Nucleic acid probes or primers for detecting esophageal cancer In the present invention, a nucleic acid capable of specifically binding to a target nucleic acid as the esophageal cancer marker is The acid can be used as a nucleic acid, such as a nucleic acid probe or primer, for detecting or diagnosing esophageal cancer. It can be used as such.

[0522] In the present invention, the use for detecting or diagnosing esophageal cancer Possible nucleic acid probes or primers include target nucleic acids such as the above-mentioned esophageal cancer markers, e.g. , human-derived hsa-miR-204-3p, hsa-miR-1247-3p, hsa -miR-6875-5p, hsa-miR-6857-5p, hsa-miR-672 6-5p, hsa-miR-3188, hsa-miR-8069, hsa-miR-4 257, hsa-miR-1343-3p, hsa-miR-7108-5p, hsa- miR-6825-5p, hsa-miR-7641, hsa-miR-3185, hs a-miR-4746-3p、hsa-miR-6791-5p、hsa-miR-68 93-5p、hsa-miR-4433b-3p、hsa-miR-3135b、hsa -miR-6781-5p、hsa-miR-1908-5p、hsa-miR-479 2, hsa-miR-7845-5p, hsa-miR-4417, hsa-miR-3 184-5p、hsa-miR-1225-5p、hsa-miR-1231、hsa- miR-1225-3p、hsa-miR-150-3p、hsa-miR-4433- 3p、hsa-miR-6125、hsa-miR-4513、hsa-miR-678 7-5p、hsa-miR-6784-5p、hsa-miR-615-5p、hsa- miR-6765-3p、hsa-miR-5572、hsa-miR-6842-5p hsa-miR-8063, hsa-miR-6780b-5p, hsa-miR-1 87-5p、hsa-miR-128-1-5p、hsa-miR-6729-5p、h sa-miR-6741-5p、hsa-miR-6757-5p、hsa-miR-7 110-5p、hsa-miR-7975、hsa-miR-1233-5p、hsa- miR-6845-5p, hsa-miR-3937, hsa-miR-4467, hs a-miR-7109-5p、hsa-miR-6088、hsa-miR-6782- 5p、hsa-miR-5195-3p、hsa-miR-4454、hsa-miR- 6724-5p、hsa-miR-8072、hsa-miR-4516、hsa-mi R-6756-5p、hsa-miR-4665-3p、hsa-miR-6826-5 p、hsa-miR-6820-5p、hsa-miR-6887-5p、hsa-mi R-3679-5p、hsa-miR-7847-3p、hsa-miR-6721-5 p、hsa-miR-3622a-5p、hsa-miR-939-5p、hsa-mi R-602、hsa-miR-7977、hsa-miR-6749-5p、hsa-m iR-1914-3p、hsa-miR-4651、hsa-miR-4695-5p、 hsa-miR-6848-5p、hsa-miR-1228-3p、hsa-miR- 642b-3p、hsa-miR-6746-5p、hsa-miR-3620-5p、 hsa-miR-3131, hsa-miR-6732-5p, hsa-miR-711 3-3p、hsa-miR-23a-3p、hsa-miR-3154、hsa-miR -4723-5p、hsa-miR-3663-3p、hsa-miR-4734、hs a-miR-6816-5p、hsa-miR-4442、hsa-miR-4476、 hsa-miR-423-5p, hsa-miR-1249, hsa-miR-6515 -3p、hsa-miR-887-3p、hsa-miR-4741、hsa-miR- 6766-3p、hsa-miR-4673、hsa-miR-6779-5p、hsa -miR-4706、hsa-miR-1268b、hsa-miR-4632-5p、 hsa-miR-3197, hsa-miR-6798-5p, hsa-miR-711 hsa-miR-6840-3p, hsa-miR-6763-5p, hsa-miR -6727-5p、hsa-miR-371a-5p、hsa-miR-6824-5p hsa-miR-4648, hsa-miR-1227-5p, hsa-miR-56 4, hsa-miR-3679-3p, hsa-miR-2861, hsa-miR-6 737-5p、hsa-miR-4725-3p、hsa-miR-6716-5p、h sa-miR-4675、hsa-miR-1915-3p、hsa-miR-671- 5p、hsa-miR-3656、hsa-miR-6722-3p、hsa-miR- 4707-5p、hsa-miR-4449、hsa-miR-1202、hsa-mi R-4649-5p、hsa-miR-744-5p、hsa-miR-642a-3p hsa-miR-451a, hsa-miR-6870-5p, hsa-miR-44 43、hsa-miR-6808-5p、hsa-miR-4728-5p、hsa-m iR-937-5p、hsa-miR-135a-3p、hsa-miR-663b、h sa-miR-1343-5p、hsa-miR-6822-5p、hsa-miR-6 803-5p、hsa-miR-6805-3p、hsa-miR-128-2-5p、 hsa-miR-4640-5p、hsa-miR-1469、hsa-miR-92a -2-5p、hsa-miR-3940-5p、hsa-miR-4281、hsa-m iR-1260b、hsa-miR-4758-5p、hsa-miR-1915-5p hsa-miR-5001-5p, hsa-miR-4286, hsa-miR-61 26、hsa-miR-6789-5p、hsa-miR-4459、hsa-miR- 1268a、hsa-miR-6752-5p、hsa-miR-6131、hsa-m iR-6800-5p, hsa-miR-4532, hsa-miR-6872-3p, hsa-miR-718, hsa-miR-6769a-5p, hsa-miR-470 7-3p, hsa-miR-6765-5p, hsa-miR-4739, hsa-mi R-4525, hsa-miR-4270, hsa-miR-4534, hsa-miR -6785-5p, hsa-miR-6850-5p, hsa-miR-4697-5p , hsa-miR-1260a, hsa-miR-4486, hsa-miR-6880 -5p, hsa-miR-6802-5p, hsa-miR-6861-5p, hsa- miR-92b-5p, hsa-miR-1238-5p, hsa-miR-6851- 5p, hsa-miR-7704, hsa-miR-149-3p, hsa-miR-4 689, hsa-miR-4688, hsa-miR-125a-3p, hsa-miR -23b-3p, hsa-miR-614, hsa-miR-1913, hsa-miR -16-5p, hsa-miR-6717-5p, hsa-miR-3648, hsa- miR-3162-5p, hsa-miR-1909-3p, hsa-miR-8073 , hsa-miR-6769b-5p, hsa-miR-6836-3p, hsa-mi R-4484, hsa-miR-6819-5p, or hsa-miR-6794- 5p or combinations thereof, or homologs thereof, transcripts thereof, or variants thereof or derivatives thereof, and optionally in combination with hsa-mi R-575 or hsa-miR-24-3p, or a combination thereof, or homologues thereof, their transcription products, their variants or derivatives, and in some cases More combinations possible, hsa-miR-675-5p, hsa-miR-48 6-3p, hsa-miR-6777-5p, hsa-miR-4497, hsa-mi R-296-3p, hsa-miR-6738-5p, hsa-miR-4731-5p , hsa-miR-6889-5p, hsa-miR-6786-5p, hsa-miR -92a-3p, hsa-miR-4294, hsa-miR-4763-3p, hsa -miR-6076, hsa-miR-663a, hsa-miR-760, hsa-m iR-4667-5p, hsa-miR-6090, hsa-miR-4730, hsa -miR-7106-5p, hsa-miR-3196, hsa-miR-5698, h sa-miR-6087, hsa-miR-4665-5p, hsa-miR-8059 or hsa-miR-6879-5p, or a combination thereof, or a homolog thereof The presence, expression level or abundance of the target gene, its transcription product, variant or derivative thereof is determined. It allows for sexual and / or quantitative measurement.

[0523] The target nucleic acid is a nucleic acid having a higher affinity for the target nucleic acid in subjects with esophageal cancer compared to healthy subjects. Depending on the species, the expression levels may increase or decrease (hereinafter, Therefore, the nucleic acid of the present invention can be used to detect the level of esophageal cancer in patients suspected of having esophageal cancer. The expression level of the target nucleic acid is measured in body fluids derived from a subject (e.g., a human) and body fluids derived from a healthy subject. These can be compared and effectively used to detect esophageal cancer.

[0524] Nucleic acid probes or primers that can be used in the present invention are those represented by SEQ ID NOs: 1 to 115, 117 to 118, 89, and at least one of the base sequences 666 to 675. Nucleic acid probes capable of specifically binding to the peptides, or SEQ ID NOs: 1 to 115, 117 to 18 9, and a polynucleotide consisting of at least one of the base sequences 666 to 675. These are primers for amplifying the gene.

[0525] Nucleic acid probes or primers that can be used in the present invention further include SEQ ID NOs: 116 and 676 A nucleic acid probe capable of specifically binding to a polynucleotide having a base sequence represented by or a primer for amplifying a polynucleotide consisting of the base sequence represented by SEQ ID NO: 116. It may contain primers.

[0526] Nucleic acid probes or primers that can be used in the present invention further include those of SEQ ID NOs: 190 to 214. A nucleic acid capable of specifically binding to a polynucleotide consisting of at least one of the base sequences represented by A probe or a base sequence represented by at least one of SEQ ID NOs: 190 to 214 The nucleic acid sequence may further comprise primers for amplifying a polynucleotide comprising the sequence.

[0527] Specifically, the nucleic acid probe or primer is any one of SEQ ID NOs: 1 to 700. or a polynucleotide containing a base sequence in which u is t in the base sequence. A group of nucleotides and their complementary polynucleotides, a base sequence complementary to the base sequence Polynucleotides that hybridize with the DNA of interest under stringent conditions (see below) Otides and their complementary polynucleotides, and the bases of those polynucleotides A group of polynucleotides containing 15 or more, preferably 17 or more consecutive bases in the sequence These polynucleotides include a combination of one or more polynucleotides selected from the following: The method comprises the steps of: preparing a nucleic acid probe and a primer for detecting the above-mentioned esophageal cancer marker, which is a target nucleic acid; It can be used as a

[0528] More specifically, examples of nucleic acid probes or primers that can be used in the present invention include the following: One or more polynucleotides selected from the group consisting of polynucleotides (a) to (e). is. (a) a sequence number represented by any one of SEQ ID NOs: 1 to 115, 117 to 189, and 666 to 675; or a polynucleotide sequence in which u is t. A nucleotide, variant, or derivative thereof is a fragment thereof containing 15 or more consecutive bases. (b) SEQ ID NOs: 1 to 115, 117 to 189, and 666 to 675 a polynucleotide comprising a base sequence (c) SEQ ID NOs: 1 to 115, 117 to 189, and 666 to 675 or a base sequence complementary to the base sequence in which u is t a polynucleotide consisting of, or a variant or derivative thereof, or a polynucleotide containing 15 or more consecutive bases Fragments of Muso, (d) SEQ ID NOs: 1 to 115, 117 to 189, and 666 to 675 or a base sequence complementary to the base sequence in which u is t and a polynucleotide comprising (e) A polynucleotide that binds to any one of the polynucleotides (a) to (d) under stringent conditions. Hybridizing polynucleotide.

[0529] The nucleic acid probe or primer that can be used in the present invention further includes the above-mentioned polynucleotide ( In addition to at least one polynucleotide selected from the group consisting of a) to (e), The polynucleotide may comprise a polynucleotide selected from the group consisting of (f) to (j). (f) the base sequence represented by SEQ ID NOs: 116 and 676 or the base sequence in which u is a polynucleotide consisting of a base sequence of t, a variant thereof, a derivative thereof, or 15 or more a fragment thereof containing consecutive bases, (g) a polynucleotide comprising the base sequences represented by SEQ ID NOs: 116 and 676; (h) the base sequence represented by SEQ ID NOs: 116 and 676, or the base sequence a polynucleotide consisting of a base sequence complementary to a base sequence in which t is a base sequence; a variant thereof; Derivatives or fragments thereof containing 15 or more consecutive bases, (i) the base sequence represented by SEQ ID NOs: 116 and 676, or the base sequence A polynucleotide comprising a base sequence complementary to a base sequence in which (j) A method for detecting a polynucleotide that is hybridized with any one of the polynucleotides (f) to (i) under stringent conditions. Hybridizing polynucleotide.

[0530] The nucleic acid probe or primer that can be used in the present invention further includes the above-mentioned polynucleotide ( In addition to at least one polynucleotide selected from the group consisting of a) to (j), The polynucleotide may comprise a polynucleotide selected from the group consisting of (k) to (o). (k) a base sequence represented by any one of SEQ ID NOs: 190 to 214 or the base sequence a polynucleotide consisting of a base sequence in which u is t, a variant thereof, a derivative thereof, or a fragment thereof containing 5 or more consecutive bases; (l) a polynucleotide comprising a nucleotide sequence represented by any one of SEQ ID NOs: 190 to 214; (m) a base sequence represented by any one of SEQ ID NOs: 190 to 214, or the base sequence a polynucleotide consisting of a base sequence complementary to the base sequence in which u is t, and a variant thereof a fragment thereof containing 15 or more consecutive bases, (n) a base sequence represented by any one of SEQ ID NOs: 190 to 214, or the base sequence a polynucleotide comprising a base sequence complementary to the base sequence in which u is t; and (o) a polynucleotide that binds to any one of the polynucleotides (k) to (n) under stringent conditions; Hybridizing polynucleotide.

[0531] In the above polynucleotide, "a fragment containing 15 or more consecutive bases" means each polynucleotide. In a base sequence of a polynucleotide, for example, 15 consecutive bases or less than the total number of bases in the sequence, It may contain a range of base numbers, such as 7 to less than the total number of bases in the sequence, or 19 to less than the total number of bases in the sequence. The present invention can be implemented in the following ways, but is not limited to these.

[0532] The polynucleotides or fragments thereof used in the present invention may be DNA. RNA may also be used.

[0533] The above polynucleotides that can be used in the present invention can be synthesized by DNA recombination techniques, PCR methods, DNA / It can be prepared using common techniques such as methods using an automatic RNA synthesizer.

[0534] DNA recombination techniques and PCR methods are described, for example, in Ausubel et al., Current Protocols tocols in Molecular Biology,John Willey& Sons, US (1993); Sambrook et al., Molecular Clonin g A Laboratory Manual,Cold Spring Harbor Laboratory Press, US (1989) and other techniques are used. It is possible.

[0535] Human-derived hsa-miR-204 represented by SEQ ID NOs: 1 to 214 and 666 to 676 -3p, hsa-miR-1247-3p, hsa-miR-6875-5p, hsa- miR-6857-5p, hsa-miR-6726-5p, hsa-miR-3188 , hsa-miR-8069, hsa-miR-4257, hsa-miR-1343- 3p, hsa-miR-7108-5p, hsa-miR-6825-5p, hsa-m iR-7641, hsa-miR-3185, hsa-miR-4746-3p, hsa -miR-6791-5p, hsa-miR-6893-5p, hsa-miR-443 3b-3p, hsa-miR-3135b, hsa-miR-6781-5p, hsa- miR-1908-5p, hsa-miR-4792, hsa-miR-7845-5p , hsa-miR-4417, hsa-miR-3184-5p, hsa-miR-12 25-5p, hsa-miR-1231, hsa-miR-1225-3p, hsa-m iR-150-3p, hsa-miR-4433-3p, hsa-miR-6125, h sa-miR-4513, hsa-miR-6787-5p, hsa-miR-6784 -5p, hsa-miR-615-5p, hsa-miR-6765-3p, hsa-m iR-5572, hsa-miR-6842-5p, hsa-miR-8063, hsa -miR-6780b-5p, hsa-miR-187-5p, hsa-miR-128 -1-5p、hsa-miR-6729-5p、hsa-miR-6741-5p、hs a-miR-6757-5p、hsa-miR-7110-5p、hsa-miR-79 75、hsa-miR-1233-5p、hsa-miR-6845-5p、hsa-m iR-3937、hsa-miR-4467、hsa-miR-7109-5p、hsa -miR-6088、hsa-miR-6782-5p、hsa-miR-5195-3 p、hsa-miR-4454、hsa-miR-6724-5p、hsa-miR-8 072、hsa-miR-4516、hsa-miR-6756-5p、hsa-miR -4665-3p、hsa-miR-6826-5p、hsa-miR-6820-5p hsa-miR-6887-5p, hsa-miR-3679-5p, hsa-miR -7847-3p、hsa-miR-6721-5p、hsa-miR-3622a-5 p、hsa-miR-939-5p、hsa-miR-602、hsa-miR-797 7、hsa-miR-6749-5p、hsa-miR-1914-3p、hsa-mi R-4651、hsa-miR-4695-5p、hsa-miR-6848-5p、h sa-miR-1228-3p、hsa-miR-642b-3p、hsa-miR-6 746-5p、hsa-miR-3620-5p、hsa-miR-3131、hsa- miR-6732-5p、hsa-miR-7113-3p、hsa-miR-23a- 3p、hsa-miR-3154、hsa-miR-4723-5p、hsa-miR- 3663-3p、hsa-miR-4734、hsa-miR-6816-5p、hsa -miR-4442、hsa-miR-4476、hsa-miR-423-5p、hs a-miR-1249, hsa-miR-6515-3p, hsa-miR-887-3 p、hsa-miR-4741、hsa-miR-6766-3p、hsa-miR-4 673、hsa-miR-6779-5p、hsa-miR-4706、hsa-miR -1268b、hsa-miR-4632-5p、hsa-miR-3197、hsa- miR-6798-5p、hsa-miR-711、hsa-miR-6840-3p、 hsa-miR-6763-5p、hsa-miR-6727-5p、hsa-miR- 371a-5p、hsa-miR-6824-5p、hsa-miR-4648、hsa -miR-1227-5p、hsa-miR-564、hsa-miR-3679-3p hsa-miR-2861, hsa-miR-6737-5p, hsa-miR-57 5、hsa-miR-4725-3p、hsa-miR-6716-5p、hsa-mi R-4675、hsa-miR-1915-3p、hsa-miR-671-5p、hs a-miR-3656、hsa-miR-6722-3p、hsa-miR-4707- 5p、hsa-miR-4449、hsa-miR-1202、hsa-miR-464 9-5p、hsa-miR-744-5p、hsa-miR-642a-3p、hsa- miR-451a, hsa-miR-6870-5p, hsa-miR-4443, hs a-miR-6808-5p、hsa-miR-4728-5p、hsa-miR-93 7-5p、hsa-miR-135a-3p、hsa-miR-663b、hsa-mi R-1343-5p、hsa-miR-6822-5p、hsa-miR-6803-5 p、hsa-miR-6805-3p、hsa-miR-128-2-5p、hsa-m iR-4640-5p、hsa-miR-1469、hsa-miR-92a-2-5p hsa-miR-3940-5p, hsa-miR-4281, hsa-miR-12 60b、hsa-miR-4758-5p、hsa-miR-1915-5p、hsa- miR-5001-5p、hsa-miR-4286、hsa-miR-6126、hs a-miR-6789-5p、hsa-miR-4459、hsa-miR-1268a hsa-miR-6752-5p, hsa-miR-6131, hsa-miR-68 00-5p、hsa-miR-4532、hsa-miR-6872-3p、hsa-m iR-718、hsa-miR-6769a-5p、hsa-miR-4707-3p、 hsa-miR-6765-5p, hsa-miR-4739, hsa-miR-452 5, hsa-miR-4270, hsa-miR-4534, hsa-miR-6785 -5p、hsa-miR-6850-5p、hsa-miR-4697-5p、hsa- miR-1260a、hsa-miR-4486、hsa-miR-6880-5p、h sa-miR-6802-5p、hsa-miR-6861-5p、hsa-miR-9 2b-5p、hsa-miR-1238-5p、hsa-miR-6851-5p、hs a-miR-7704、hsa-miR-149-3p、hsa-miR-4689、h sa-miR-4688、hsa-miR-125a-3p、hsa-miR-23b- 3p、hsa-miR-614、hsa-miR-1913、hsa-miR-16-5 p、hsa-miR-6717-5p、hsa-miR-3648、hsa-miR-3 162-5p, hsa-miR-1909-3p, hsa-miR-8073, hsa- miR-6769b-5p, hsa-miR-6836-3p, hsa-miR-448 4, hsa-miR-6819-5p, hsa-miR-6794-5phsa-miR -675-5p, hsa-miR-24-3p, hsa-miR-486-3p, hsa -miR-6777-5p, hsa-miR-4497, hsa-miR-296-3p , hsa-miR-6738-5p, hsa-miR-4731-5p, hsa-miR -6889-5p, hsa-miR-6786-5p, hsa-miR-92a-3p, hsa-miR-4294, hsa-miR-4763-3p, hsa-miR-607 6, hsa-miR-663a, hsa-miR-760, hsa-miR-4667- 5p, hsa-miR-6090, hsa-miR-4730, hsa-miR-710 6-5p, hsa-miR-3196, hsa-miR-5698, hsa-miR-6 087, hsa-miR-4665-5p, hsa-miR-8059 and hsa-mi R-6879-5p is publicly known, and as mentioned above, the method for obtaining it is also known. By cloning this gene, a nucleic acid probe or primer that can be used in the present invention can be obtained. A polynucleotide can be prepared as a primer.

[0536] Such nucleic acid probes or primers can be chemically synthesized using an automated DNA synthesizer. This synthesis is generally carried out using the phosphoramidite method, which The automated DNA synthesizer can automatically synthesize single-stranded DNA up to about 100 bases. For example, Polygen, ABI, Applied BioSystems, etc. It is commercially available.

[0537] Alternatively, the polynucleotides of the present invention can be produced by cDNA cloning techniques. cDNA cloning technology is also possible, for example, microRNA cloning You can use Kit Wako etc.

[0538] Here, the nucleic acid sequence is composed of a base sequence represented by any one of SEQ ID NOs: 1 to 214 and 666 to 676. The sequences of the nucleic acid probes and primers for detecting the polynucleotides Or, it does not exist in vivo as a precursor thereof. For example, SEQ ID NO: 9 and SEQ ID NO: 13 The base sequence represented by SEQ ID NO: 8 is generated from the precursor represented by SEQ ID NO: 223. The peptide has a hairpin-like structure as shown in FIG. 1 and is represented by SEQ ID NO: 9 and SEQ ID NO: 138. Similarly, the base sequences of SEQ ID NO: 9 and SEQ ID NO: 10 have mismatch sequences. The perfectly complementary base sequence to the base sequence represented by 138 is naturally produced in vivo. Therefore, the sequences represented by any of SEQ ID NOs: 1 to 214 and 666 to 676 are not used. The nucleic acid probes and primers used to detect base sequences are artificial salts that do not exist in living organisms. It will have a base sequence. 3. Esophageal cancer detection kit or device The present invention also relates to a method for measuring a target nucleic acid that is an esophageal cancer marker, comprising the steps of: Polynucleotides that can be used as nucleic acid probes or primers (including mutants, fragments, or a derivative thereof (hereinafter sometimes referred to as a detection polynucleotide). or a plurality of kits or devices for detecting esophageal cancer.

[0539] The target nucleic acid that is an esophageal cancer marker in the present invention is selected from Group A below.

[0540] (Group A) hsa-miR-204-3p, hsa-miR-1247-3p, hsa- miR-6875-5p, hsa-miR-6857-5p, hsa-miR-6726 -5p, hsa-miR-3188, hsa-miR-8069, hsa-miR-42 57, hsa-miR-1343-3p, hsa-miR-7108-5p, hsa-m iR-6825-5p, hsa-miR-7641, hsa-miR-3185, hsa -miR-4746-3p, hsa-miR-6791-5p, hsa-miR-689 3-5p, hsa-miR-4433b-3p, hsa-miR-3135b, hsa- miR-6781-5p, hsa-miR-1908-5p, hsa-miR-4792 , hsa-miR-7845-5p, hsa-miR-4417, hsa-miR-31 84-5p, hsa-miR-1225-5p, hsa-miR-1231, hsa-m iR-1225-3p, hsa-miR-150-3p, hsa-miR-4433-3 p, hsa-miR-6125, hsa-miR-4513, hsa-miR-6787 -5p, hsa-miR-6784-5p, hsa-miR-615-5p, hsa-m iR-6765-3p, hsa-miR-5572, hsa-miR-6842-5p, hsa-miR-8063, hsa-miR-6780b-5p, hsa-miR-18 7-5p, hsa-miR-128-1-5p, hsa-miR-6729-5p, hs a-miR-6741-5p, hsa-miR-6757-5p, hsa-miR-71 10-5p、hsa-miR-7975、hsa-miR-1233-5p、hsa-m iR-6845-5p、hsa-miR-3937、hsa-miR-4467、hsa -miR-7109-5p、hsa-miR-6088、hsa-miR-6782-5 p、hsa-miR-5195-3p、hsa-miR-4454、hsa-miR-6 724-5p、hsa-miR-8072、hsa-miR-4516、hsa-miR -6756-5p、hsa-miR-4665-3p、hsa-miR-6826-5p hsa-miR-6820-5p, hsa-miR-6887-5p, hsa-miR -3679-5p、hsa-miR-7847-3p、hsa-miR-6721-5p 、hsa-miR-3622a-5p、hsa-miR-939-5p、hsa-miR -602、hsa-miR-7977、hsa-miR-6749-5p、hsa-mi R-1914-3p、hsa-miR-4651、hsa-miR-4695-5p、h sa-miR-6848-5p、hsa-miR-1228-3p、hsa-miR-6 42b-3p、hsa-miR-6746-5p、hsa-miR-3620-5p、h sa-miR-3131、hsa-miR-6732-5p、hsa-miR-7113 -3p、hsa-miR-23a-3p、hsa-miR-3154、hsa-miR- 4723-5p、hsa-miR-3663-3p、hsa-miR-4734、hsa -miR-6816-5p、hsa-miR-4442、hsa-miR-4476、h sa-miR-423-5p、hsa-miR-1249、hsa-miR-6515- 3p、hsa-miR-887-3p、hsa-miR-4741、hsa-miR-6 766-3p、hsa-miR-4673、hsa-miR-6779-5p、hsa- miR-4706, hsa-miR-1268b, hsa-miR-4632-5p, h sa-miR-3197、hsa-miR-6798-5p、hsa-miR-711、 hsa-miR-6840-3p、hsa-miR-6763-5p、hsa-miR- 6727-5p、hsa-miR-371a-5p、hsa-miR-6824-5p、 hsa-miR-4648, hsa-miR-1227-5p, hsa-miR-564 hsa-miR-3679-3p, hsa-miR-2861, hsa-miR-67 37-5p、hsa-miR-4725-3p、hsa-miR-6716-5p、hs a-miR-4675、hsa-miR-1915-3p、hsa-miR-671-5 p、hsa-miR-3656、hsa-miR-6722-3p、hsa-miR-4 707-5p、hsa-miR-4449、hsa-miR-1202、hsa-miR -4649-5p、hsa-miR-744-5p、hsa-miR-642a-3p、 hsa-miR-451a, hsa-miR-6870-5p, hsa-miR-444 3、hsa-miR-6808-5p、hsa-miR-4728-5p、hsa-mi R-937-5p、hsa-miR-135a-3p、hsa-miR-663b、hs a-miR-1343-5p、hsa-miR-6822-5p、hsa-miR-68 03-5p、hsa-miR-6805-3p、hsa-miR-128-2-5p、h sa-miR-4640-5p、hsa-miR-1469、hsa-miR-92a- 2-5p、hsa-miR-3940-5p、hsa-miR-4281、hsa-mi R-1260b、hsa-miR-4758-5p、hsa-miR-1915-5p、 hsa-miR-5001-5p、hsa-miR-4286、hsa-miR-612 6, hsa-miR-6789-5p, hsa-miR-4459, hsa-miR-1 268a、hsa-miR-6752-5p、hsa-miR-6131、hsa-mi R-6800-5p、hsa-miR-4532、hsa-miR-6872-3p、h sa-miR-718, hsa-miR-6769a-5p, hsa-miR-4707 -3p、hsa-miR-6765-5p、hsa-miR-4739、hsa-miR -4525、hsa-miR-4270、hsa-miR-4534、hsa-miR- 6785-5p、hsa-miR-6850-5p、hsa-miR-4697-5p、 hsa-miR-1260a、hsa-miR-4486、hsa-miR-6880- 5p、hsa-miR-6802-5p、hsa-miR-6861-5p、hsa-m iR-92b-5p、hsa-miR-1238-5p、hsa-miR-6851-5 p、hsa-miR-7704、hsa-miR-149-3p、hsa-miR-46 89、hsa-miR-4688、hsa-miR-125a-3p、hsa-miR- 23b-3p、hsa-miR-614、hsa-miR-1913、hsa-miR- 16-5p、hsa-miR-6717-5p、hsa-miR-3648、hsa-m iR-3162-5p、hsa-miR-1909-3p、hsa-miR-8073、 hsa-miR-6769b-5p、hsa-miR-6836-3p、hsa-miR -4484, hsa-miR-6819-5p, and hsa-miR-6794-5p Additional target nucleic acids that may optionally be used for measurement are selected from Group B below. (Group B) hsa-miR-575 and hsa-miR-24-3p Additional target nucleic acids that may optionally be used for measurement are selected from Group C below. (Group C) hsa-miR-675-5p, hsa-miR-486-3p, hsa-mi R-6777-5p, hsa-miR-4497, hsa-miR-296-3p, hs a-miR-6738-5p, hsa-miR-4731-5p, hsa-miR-68 89-5p, hsa-miR-6786-5p, hsa-miR-92a-3p, hsa -miR-4294, hsa-miR-4763-3p, hsa-miR-6076, h sa-miR-663a, hsa-miR-760, hsa-miR-4667-5p, hsa-miR-6090, hsa-miR-4730, hsa-miR-7106-5 p, hsa-miR-3196, hsa-miR-5698, hsa-miR-6087 , hsa-miR-4665-5p, hsa-miR-8059 and hsa-miR-6 879-5p The kit or device of the present invention specifically binds to the target nucleic acid, which is the esophageal cancer marker. a nucleic acid capable of synthesizing the nucleic acid, preferably one or more selected from the polynucleotides described in 2 above; It includes multiple polynucleotides or variants thereof.

[0541] Specifically, the kit or device of the present invention includes SEQ ID NOs: 1 to 115, 117 to 189, and a base sequence represented by any one of 666 to 675, or the base sequence a polynucleotide containing (or consisting of) a base sequence in which t is a complementary sequence Polynucleotides comprising (or consisting of) Polynucleotides that hybridize under gentle conditions, or those polynucleotides A variant or fragment containing 15 or more consecutive bases of the sequence may be included. do.

[0542] The kit or device of the present invention further comprises a nucleic acid sequence represented by SEQ ID NOs: 116 and 676. or a base sequence in which u is t in the base sequence (or consisting of) Polynucleotides, polynucleotides containing (or consisting of) their complementary sequences, Polynucleotides that hybridize with these polynucleotides under stringent conditions or variants or fragments containing 15 or more consecutive bases of these polynucleotide sequences , can contain one or more.

[0543] The kit or device of the present invention further comprises a compound represented by any one of SEQ ID NOs: 190 to 214. A base sequence, or a base sequence in which u is t in the base sequence (or a polynucleotide comprising (or consisting of) its complementary sequence; Polynucleotides that hybridize under stringent conditions with these polynucleotides. nucleotides or variants containing 15 or more consecutive bases of their polynucleotide sequences or fragments thereof.

[0544] The fragments that can be contained in the kit or device of the present invention include, for example, the following fragments (1) to (3): The polynucleotide is one or more, preferably two or more, selected from the group consisting of: (1) Represented by any of SEQ ID NOs: 1 to 115, 117 to 189, and 666 to 675 In the base sequence in which u is t or its complementary sequence, 15 or more consecutive A polynucleotide containing bases. (2) a base sequence in which u is t in the base sequences represented by SEQ ID NOs: 116 and 676; or A polynucleotide containing 15 or more consecutive bases in its complementary sequence. (3) A base in which u is t in the base sequence represented by any one of SEQ ID NOs: 190 to 214 A polynucleotide containing 15 or more consecutive bases in a sequence or its complementary sequence.

[0545] In a preferred embodiment, the polynucleotide is selected from the group consisting of SEQ ID NOs: 1 to 115, 117 to 18 9, and the base sequence represented by any one of 666 to 675, or the base sequence a polynucleotide consisting of a base sequence in which u is t, a polynucleotide consisting of a complementary sequence thereof, Polynucleotides that hybridize under stringent conditions to these polynucleotides. nucleotides, or 15 or more thereof, preferably 17 or more, more preferably 19 or more thereof It is a mutant containing consecutive bases.

[0546] In a preferred embodiment, the polynucleotide is selected from the group consisting of SEQ ID NOs: 116 and 676. A base sequence represented by either of these, or a base sequence in which u is t in the base sequence, or a polynucleotide consisting of the sequence, a polynucleotide consisting of the sequence complementary thereto, Polynucleotides that hybridize to the nucleotide under stringent conditions, or A mutant containing 15 or more, preferably 17 or more, more preferably 19 or more consecutive bases of is.

[0547] In a preferred embodiment, the polynucleotide is any one of SEQ ID NOs: 190 to 214. A base sequence represented by either of the above, or a base sequence in which u is t in the base sequence, a polynucleotide comprising the sequence thereof, a polynucleotide comprising the sequence thereof, Polynucleotides that hybridize under stringent conditions with the nucleotides, or their A mutant containing 15 or more, preferably 17 or more, more preferably 19 or more consecutive bases. be.

[0548] In a preferred embodiment, the fragments are 15 or more, preferably 17 or more, more preferably It can be a polynucleotide containing 19 or more consecutive bases.

[0549] In the present invention, the size of a polynucleotide fragment is determined based on the base sequence of each polynucleotide. For example, from 15 to less than the total number of bases in the sequence, from 17 to less than the total number of bases in the sequence , the number of bases may range from 19 to less than the total number of bases in the sequence.

[0550] The combination of the above polynucleotides constituting the kit or device of the present invention may be: Specifically, the combinations of SEQ ID NOs: 1 to 214 and 666 to 676 shown in Table 1 The polynucleotides may be 2, 3, 4, 5, 6, 7, 8, 9, 10 or more. There are more combinations than the above, but these are merely examples. , and all other various possible combinations are intended to be encompassed by the present invention.

[0551] For example, in the present invention, a kit or device for distinguishing between esophageal cancer and healthy subjects is constructed. The above combinations include those represented by SEQ ID NOs: 1 to 214 and 666 to 676 shown in Table 1. It is desirable to combine two or more of the above polynucleotides having the base sequence Usually, a combination of two can provide sufficient performance.

[0552] Specifically, the two polynucleotide combinations used to distinguish between esophageal cancer and healthy subjects are: The above polynucleotides consisting of the base sequences represented by SEQ ID NOs: 1 to 214 and 666 to 676 Among the two combinations consisting of leucine and methionine, the newly discovered SEQ ID NOs: 1 to 115, A polynucleotide consisting of the base sequences represented by 117 to 189 and 666 to 675 is A combination containing at least one of these is preferred.

[0553] In addition, we have developed a cancer-specific polymerase chain reaction (PCR) that can distinguish esophageal cancer from not only healthy individuals but also other cancers. As a combination of oligonucleotides, for example, SEQ ID NOs: 1, 2, 5, 8, 22, 32, 33 , 35, 43, 44, 56, 85, 98, 106, 109, 115, 121, 126, 1 33, 138, 155, 157, 166, 177, 179, 185, 202, 212, 6 66, 667, 668, 669, 670, 671, 672, 673, 674, 675 and A group consisting of polynucleotides with a base sequence represented by 676 (hereinafter, this group is referred to as "cancer type" At least one polynucleotide selected from the "specific polynucleotide group 1" A combination of multiple polynucleotides of the same sequence number and other sequences is preferred.

[0554] More preferred combinations of cancer type-specific polynucleotides include: A combination of multiple polynucleotides selected from cancer type-specific polynucleotide group 1 That is it.

[0555] Further, a more preferred combination of cancer type-specific polynucleotides is The combination is a combination of a plurality of polynucleotides selected from Cancer Type-Specific Polynucleotide Group 1. Among the combinations, SEQ ID NOs: 1, 22, and 32 are included in cancer type-specific polynucleotide group 1. Nucleotide sequences represented by 85, 109, 121, 126, 133, 138, 166, and 666 A group consisting of polynucleotides consisting of the following (hereinafter, this group is referred to as "cancer type-specific polynucleotide group 2" ").

[0556] The number of combinations of the above cancer type-specific polynucleotides is 1, 2, or , 3, 4, 5, 6, 7, 8, 9, 10 or more combinations However, more preferably, six or more combinations are possible, and usually six combinations are possible. This allows for sufficient performance.

[0557] The following are non-limiting examples of sequences consisting of the base sequence represented by SEQ ID NO: 1 or its complementary sequence: Polynucleotide and five polynucleotides selected from cancer type-specific polynucleotide group 1 A polynucleotide consisting of a base sequence represented by the SEQ ID NO of the nucleotide or its complementary sequence. The following illustrates a combination of: (1) SEQ ID NOs: 1, 85, 138, 166, 666, and 668 (marker: hsa-m iR-4739, hsa-miR-1343-5p, hsa-miR-204-3p, h sa-miR-4723-5p, hsa-miR-3162-5p, and hsa-miR -6717-5p) (2) SEQ ID NOs: 1, 85, 98, 138, 166, and 666 (marker: hsa-mi R-4739, hsa-miR-1343-5p, hsa-miR-6779-5p, h sa-miR-204-3p, hsa-miR-4723-5p, and hsa-miR- A combination of polynucleotides consisting of the base sequence represented by (3) SEQ ID NOs: 1, 85, 138, 155, 166, and 666 (marker: hsa-m iR-4739, hsa-miR-1343-5p, hsa-miR-204-3p, h sa-miR-4723-5p, hsa-miR-4459, and hsa-miR-67 A combination of polynucleotides consisting of the base sequence represented by 17-5p (4) SEQ ID NOs: 1, 5, 85, 138, 166, and 666 (marker: hsa-miR -4739, hsa-miR-1343-5p, hsa-miR-204-3p, hsa -miR-4723-5p, hsa-miR-6726-5p, and hsa-miR-6 A combination of polynucleotides consisting of the base sequence represented by (5) SEQ ID NOs: 1, 35, 85, 138, 166, and 666 (marker: hsa-mi R-4739, hsa-miR-1343-5p, hsa-miR-204-3p, hs a-miR-4723-5p, hsa-miR-6765-3p, and hsa-miR- A combination of polynucleotides consisting of the base sequence represented by Furthermore, the present invention also includes, but is not limited to, a nucleic acid sequence comprising the base sequence represented by SEQ ID NO: 22 or a complementary sequence thereof. and five polynucleotides selected from cancer type-specific polynucleotide group 1. A polynucleotide consisting of a base sequence represented by the sequence number of the nucleic acid fragment or a complementary sequence thereof. Examples of combinations with this are shown below. (1) SEQ ID NOs: 1, 22, 85, 138, 166, and 666 (marker: hsa-mi R-4739, hsa-miR-1343-5p, hsa-miR-7845-5p, h sa-miR-204-3p, hsa-miR-4723-5p, and hsa-miR- 6717-5p) combination (2) SEQ ID NOs: 22, 32, 121, 133, 166, and 666 (marker: hsa- miR-4739, hsa-miR-7845-5p, hsa-miR-671-5p, hsa-miR-6787-5p, hsa-miR-6808-5p, and hsa-mi R-6717-5p) (3) SEQ ID NOs: 1, 22, 126, 138, 166, and 666 (marker: hsa-m iR-4739, hsa-miR-1202, hsa-miR-1343-5p, hsa -miR-7845-5p, hsa-miR-204-3p, and hsa-miR-67 A combination of polynucleotides consisting of the base sequence represented by 17-5p (4) SEQ ID NOs: 1, 22, 121, 155, 166, and 666 (marker: hsa-m iR-4739, hsa-miR-7845-5p, hsa-miR-671-5p, h sa-miR-204-3p, hsa-miR-4459, and hsa-miR-671 7-5p) (5) SEQ ID NOs: 22, 32, 109, 121, 666, and 667 (marker: hsa- miR-7845-5p, hsa-miR-671-5p, hsa-miR-3648, hsa-miR-6787-5p, hsa-miR-6824-5p, and hsa-mi R-6717-5p) Furthermore, the present invention also includes, but is not limited to, a nucleic acid sequence comprising the base sequence represented by SEQ ID NO: 85 or a complementary sequence thereof. and five polynucleotides selected from cancer type-specific polynucleotide group 1. A polynucleotide consisting of a base sequence represented by the sequence number of the nucleic acid fragment or a complementary sequence thereof. Examples of combinations with this are shown below. (1) SEQ ID NOs: 85, 138, 166, 185, 666, and 669 markers: miR- 4739, miR-1343-5p, miR-125a-3p, miR-4723-5p , miR-1909-3p, and miR-6717-5p) Polynucleotide Combinations (2) SEQ ID NOs: 85, 138, 166, 185, 666, and 676 (marker: miR -4739, miR-1343-5p, miR-125a-3p, miR-4723-5 p, miR-6717-5p, and miR-24-3p) Ligand combinations (3) SEQ ID NOs: 85, 138, 166, 177, 185, and 666 (marker: miR -4739, miR-1343-5p, miR-125a-3p, miR-4723-5 p, miR-6861-5p, and miR-6717-5p) Polynucleotide combinations (4) SEQ ID NOs: 85, 138, 166, 185, 666, and 667 (marker: miR -4739, miR-1343-5p, miR-3648, miR-125a-3p, m miR-4723-5p, and miR-6717-5p) Nucleotide combinations (5) SEQ ID NOs: 33, 85, 138, 166, 185, and 666 (marker: miR- 6784-5p, miR-4739, miR-1343-5p, miR-125a-3p , miR-4723-5p, and miR-6717-5p) Polynucleotide Combinations Furthermore, without limitation, the base sequence represented by SEQ ID NO: 109 or its complementary sequence and five polynucleotides selected from cancer type-specific polynucleotide group 1. A polynucleotide consisting of a base sequence represented by the sequence number of a nucleotide or a complementary sequence thereof. The following shows an example of a combination with chido. (1) SEQ ID NOs: 109, 121, 126, 138, 166, and 666 (marker: mi R-4739, miR-1202, miR-1343-5p, miR-671-5p, m miR-6824-5p, and miR-6717-5p) Nucleotide combinations (2) SEQ ID NOs: 1, 85, 109, 138, 166, and 666 (marker: miR-4 739, miR-1343-5p, miR-204-3p, miR-4723-5p, m A polynucleotide consisting of a base sequence represented by miR-6824-5p, miR-6717-5p Reotide combination (3) SEQ ID NOs: 1, 109, 121, 138, 166, and 666 (marker: miR- 4739, miR-1343-5p, miR-671-5p, miR-204-3p, m miR-6824-5p, and miR-6717-5p) Nucleotide combinations (4) SEQ ID NOs: 109, 126, 138, 166, 666, and 676 (marker: mi R-4739, miR-1202, miR-1343-5p, miR-6824-5p, miR-6717-5p, and miR-24-3p) Nucleotide combination (5) SEQ ID NOs: 109, 126, 138, 166, 202, and 666 (marker: mi R-4739, miR-1202, miR-1343-5p, miR-6824-5p, miR-6076 and miR-6717-5p) Reotide combination Furthermore, it is not limited to a nucleic acid sequence consisting of the base sequence represented by SEQ ID NO: 121 or a complementary sequence thereof. and five polynucleotides selected from cancer type-specific polynucleotide group 1. A polynucleotide consisting of a base sequence represented by the sequence number of the nucleic acid fragment or a complementary sequence thereof. Examples of combinations with this are shown below. (1) SEQ ID NOs: 1, 121, 138, 166, 666, and 668 (marker: miR- 4739, miR-1343-5p, miR-671-5p, miR-204-3p, m miR-3162-5p, and miR-6717-5p) Nucleotide combinations (2) SEQ ID NOs: 1, 33, 121, 138, 166, and 666 (marker: miR-6 784-5p, miR-4739, miR-1343-5p, miR-671-5p, m Polynucleotides consisting of base sequences represented by miR-204-3p and miR-6717-5p Nucleotide combination (3) SEQ ID NOs: 1, 85, 121, 138, 166, and 666 (marker: miR-4 739, miR-1343-5p, miR-671-5p, miR-204-3p, mi miR-4723-5p, and miR-6717-5p) Nucleotide combination (4) SEQ ID NOs: 1, 121, 138, 166, 179, and 666 (marker: miR- 4739, miR-1343-5p, miR-671-5p, miR-204-3p, m miR-1238-5p, and miR-6717-5p) Nucleotide combinations (5) SEQ ID NOs: 1, 121, 138, 166, 177, and 666 (marker: miR- 4739, miR-1343-5p, miR-671-5p, miR-204-3p, m miR-6861-5p, and miR-6717-5p) Nucleotide combinations Furthermore, without limitation, the base sequence represented by SEQ ID NO: 126 or its complementary sequence and five polynucleotides selected from cancer type-specific polynucleotide group 1. A polynucleotide consisting of a base sequence represented by the sequence number of a nucleotide or a complementary sequence thereof. The following shows an example of a combination with chido. (1) SEQ ID NOs: 32, 109, 126, 138, 166, and 666 (marker: miR -4739, miR-1202, miR-1343-5p, miR-6787-5p, m miR-6824-5p, and miR-6717-5p) Nucleotide combinations (2) SEQ ID NOs: 1, 85, 126, 138, 166, and 666 (marker: miR-4 739, miR-1202, miR-1343-5p, miR-204-3p, miR- miR-4723-5p, and miR-6717-5p) Combination of ochido (3) SEQ ID NOs: 1, 109, 126, 138, 166, and 666 (marker: miR- 4739, miR-1202, miR-1343-5p, miR-204-3p, miR miR-6824-5p, and miR-6717-5p) Reotide combination (4) SEQ ID NOs: 22, 109, 126, 138, 166, and 666 (marker: miR -4739, miR-1202, miR-1343-5p, miR-7845-5p, m miR-6824-5p, and miR-6717-5p) Nucleotide combinations (5) SEQ ID NOs: 109, 126, 138, 157, 166, and 666 (marker: mi R-4739, miR-1202, miR-6752-5p, miR-1343-5p, miR-6824-5p and miR-6717-5p) Furthermore, it is not limited to a nucleic acid sequence consisting of the base sequence represented by SEQ ID NO: 133 or a complementary sequence thereof. and five polynucleotides selected from cancer type-specific polynucleotide group 1. A polynucleotide consisting of a base sequence represented by the sequence number of the nucleic acid fragment or a complementary sequence thereof. Examples of combinations with this are shown below. (1) SEQ ID NOs: 126, 133, 138, 166, 666, and 672 (marker: mi R-4739, miR-1202, miR-1343-5p, miR-6808-5p, miR-6836-3p, and miR-6717-5p) Ligand combinations (2) SEQ ID NOs: 126, 133, 138, 166, 666 (marker: miR-473 9, miR-1202, miR-1343-5p, miR-6808-5p, and miR -6717-5p,) a combination of polynucleotides consisting of a base sequence (3) SEQ ID NOs: 109, 126, 133, 138, 166, and 666 (marker: mi Polynucleotides consisting of the base sequences represented by miR-4739, miR-1202, and miR-1343 nucleotide-5p, miR-6824-5p, miR-6808-5p, and miR-6 717-5p) combination (4) SEQ ID NOs: 126, 133, 138, 166, 666, and 673 (marker: mi R-4739, miR-1202, miR-1343-5p, miR-4484, miR -6808-5p, and miR-6717-5p) (5) SEQ ID NOs: 126, 133, 138, 166, 666, and 675 (marker: mi R-4739, miR-1202, miR-1343-5p, miR-6794-5p, miR-6808-5p and miR-6717-5p) Ligand combinations Furthermore, without limitation, the base sequence represented by SEQ ID NO: 138 or its complementary sequence and five polynucleotides selected from cancer type-specific polynucleotide group 1. A polynucleotide consisting of a base sequence represented by the sequence number of a nucleotide or a complementary sequence thereof. The following shows an example of a combination with chido. (1) SEQ ID NOs: 1, 85, 138, 166, 666, and 669 (marker: miR-4 739, miR-1343-5p, miR-204-3p, miR-4723-5p, m miR-1909-3p, and miR-6717-5p) Nucleotide combinations (2) SEQ ID NOs: 8, 85, 138, 166, 185, and 666 (marker: miR-4 739, miR-1343-5p, miR-125a-3p, miR-4723-5p, miR-4257 and miR-6717-5p) Reotide combination (3) SEQ ID NOs: 1, 35, 121, 138, 166, and 666 (marker: miR-4 739, miR-1343-5p, miR-671-5p, miR-204-3p, mi miR-6765-3p, and miR-6717-5p) Nucleotide combination (4) SEQ ID NOs: 1, 121, 126, 138, 166, and 666 (marker: miR- 4739, miR-1202, miR-1343-5p, miR-671-5p, miR miR-204-3p, and miR-6717-5p) Combination of ochido (5) SEQ ID NOs: 1, 121, 138, 166, 666, and 672 (marker: miR- 4739, miR-1343-5p, miR-671-5p, miR-204-3p, m miR-6836-3p, and miR-6717-5p) Nucleotide combinations Furthermore, without limitation, the base sequence represented by SEQ ID NO: 166 or its complementary sequence and five polynucleotides selected from cancer type-specific polynucleotide group 1. A polynucleotide consisting of a base sequence represented by the sequence number of a nucleotide or a complementary sequence thereof. The following shows an example of a combination with chido. (1) SEQ ID NOs: 1, 85, 138, 166, 666, and 672 (marker: miR-4 739, miR-1343-5p, miR-204-3p, miR-4723-5p, m miR-6836-3p, and miR-6717-5p) Nucleotide combinations (2) SEQ ID NOs: 56, 85, 138, 166, 185, and 666 (marker: miR- 4739, miR-1343-5p, miR-125a-3p, miR-6724-5p , miR-4723-5p, and miR-6717-5p) Polynucleotide Combinations (3) SEQ ID NOs: 1, 32, 121, 138, 166, and 666 (marker: miR-4 739, miR-1343-5p, miR-671-5p, miR-204-3p, mi miR-6787-5p and miR-6717-5p) Nucleotide combination (4) SEQ ID NOs: 1, 22, 121, 138, 166, and 666 (marker: miR-4 739, miR-1343-5p, miR-7845-5p, miR-671-5p, m Polynucleotides consisting of base sequences represented by miR-204-3p and miR-6717-5p Nucleotide combination (5) SEQ ID NOs: 5, 85, 138, 166, 185, and 666 (marker: miR-4 739, miR-1343-5p, miR-125a-3p, miR-4723-5p, miR-6726-5p and miR-6717-5p) Ligand combinations Furthermore, without limitation, the base sequence represented by SEQ ID NO: 666 or its complementary sequence and five polynucleotides selected from cancer type-specific polynucleotide group 1. A polynucleotide consisting of a base sequence represented by the sequence number of a nucleotide or a complementary sequence thereof. The following shows an example of a combination with chido. (1) SEQ ID NOs: 1, 121, 138, 157, 166, and 666 (marker: miR- 4739, miR-6752-5p, miR-1343-5p, miR-671-5p, miR-204-3p, and miR-6717-5p) Nucleotide combinations (2) SEQ ID NOs: 1, 85, 133, 138, 166, and 666 (marker: miR-4 739, miR-1343-5p, miR-204-3p, miR-4723-5p, m miR-6808-5p and miR-6717-5p) Nucleotide combinations (3) SEQ ID NOs: 1, 121, 138, 166, 185, and 666 (marker: miR- 4739, miR-1343-5p, miR-671-5p, miR-204-3p, m miR-125a-3p, and miR-6717-5p) Nucleotide combinations (4) SEQ ID NOs: 1, 121, 138, 166, 666, and 667 (marker: miR- 4739, miR-1343-5p, miR-671-5p, miR-3648, miR miR-204-3p, and miR-6717-5p) Combination of ochido (5) SEQ ID NOs: 85, 138, 166, 185, and 666 (marker: miR-473 9, miR-1343-5p, miR-125a-3p, miR-4723-5p, and miR-6717-5p, ) combination The kit or device of the present invention may contain the polynucleotide of the present invention described above ( This may include mutants, fragments, or derivatives. It may include known polynucleotides or polynucleotides that may be discovered in the future. Cut.

[0558] The kit of the present invention includes, in addition to the polynucleotide of the present invention described above, Antibodies for measuring known esophageal cancer markers such as EA and SCC may also be included. can.

[0559] The above polynucleotides contained in the kit of the present invention may be used individually or in any combination. It can be packaged in a container.

[0560] The kit of the present invention includes a method for extracting nucleic acids (e.g., total RNA) from b...

Claims

1. A kit for detecting esophageal cancer, comprising a nucleic acid probe capable of specifically binding to a polynucleotide of miR-6784-5p, which is an esophageal cancer marker and has the base sequence represented by SEQ ID NO: 33, and / or a primer that specifically recognizes and amplifies the polynucleotide.

2. The kit is another esophageal cancer marker, and consists of miR-1247-3p, miR-6875-5p, miR-6726-5p, miR-3188, miR-8069, miR-1343-3p, miR-7108-5p, miR-6825-5p, miR-7641, miR-3185, miR-4746-3p, miR-6791-5p, miR-6893-5p, miR-4433b-3p, miR-3135b, miR-6781-5p, miR-1908-5p, miR-4792, miR-7845-5p, miR-4417, miR-3184-5p, miR-1225-5p, miR-1231, miR-1225-3p, miR-150-3p, miR-4433-3p, miR-6125, miR-4513, miR-6787-5p, miR-6765-3p, miR-5572, miR-6842-5p, miR-8063, miR-6780b-5p, miR-187-5p, miR-128-1-5p, miR-6729-5p, miR-6741-5p, miR-6757-5p, miR-7110-5p, miR-7975, miR-1233-5p, miR-6845-5p, miR-3937, miR-4467, miR-7109-5p, miR-6088, miR-6782-5p, miR-5195-3p, miR-4454, miR-6724-5p, miR-8072, miR-4516, miR-6756-5p, miR-4665-3p, miR-6826-5p, miR-6820-5p, miR-6887-5p, miR-3679-5p, miR-7847-3p, miR-6721-5p, miR-3622a-5p, miR-939-5p, miR-602, miR-7977, miR-6749-5p, miR-1914-3p, miR-4651, miR-4695-5p, miR-6848-5p, miR-1228-3p, miR-642b-3p, miR-6746-5p, miR-3620-5p, miR-3131, miR-6732-5p, miR-7113-3p, miR-23a-3p, miR-3154, miR-4723-5p, miR-3663-3p, miR-4734, miR-6816-5p, miR-4442, which are composed of the base sequences represented by SEQ ID NOs: 2 to 3, 5 to 7, 9 to 32, 35 to 214, and 666 to 676 respectively.miR-4476、miR-423-5p、miR-1249、miR-6515-3p、miR-4741、m iR-6766-3p、miR-4673、miR-6779-5p、miR-4706、miR-1268b、miR-4632-5p 、miR-3197、miR-6798-5p、miR-711、miR-6840-3p、miR-6763-5p、miR-6727 -5p、miR-371a-5p、miR-6824-5p、miR-4648、miR-1227-5p、miR-564、miR-36 79-3p、miR-2861、miR-6737-5p、miR-4675、mi R-1915-3p、miR-671-5p、miR-3656、miR-6722-3p、miR-4707-5p、miR-449 、miR-1202、miR-4649-5p、miR-744-5p、miR-642a-3p、miR-451a、miR-6870 -5p、miR-443、miR-6808-5p、miR-4728-5p、miR-937-5p、miR-135a-3p、miR -663b、miR-1343-5p、miR-6822-5p、miR-6803-5p、miR-128- 2-5p、miR-4640-5p、miR-1469、miR-92a-2-5p、miR-3940-5p、miR-4281、mi R-1260b、miR-4758-5p、miR-1915-5p、miR-4286、miR-6126、 miR-6789-5p、miR-4459、miR-1268a、miR-6752-5p、miR-6131、miR-6800-5p 、miR-4532、miR-6872-3p、miR-718、miR-6769a-5p、miR-4707-3p、miR-676 5-5p、miR-4739、miR-4525、miR-4270、miR-4534、miR-6785-5p、miR-6850- 5p、miR-4697-5p、miR-1260a、miR-4486、miR-680-5p、miR- 6861-5p、miR-92b-5p、miR-1238-5p、miR-6851-5p、miR-7704、miR-149-3p、miR-4689, miR-4688, miR-125a-3p, miR-23b-3p, miR-614, miR-1913, miR-16-5p, m iR-6717-5p, miR-3648, miR-3162-5p, miR-1909-3p, miR-8073, miR-6769b-5p, miR -6836-3p, miR-4484, miR-6819-5p and miR-6794-5p, miR-575, miR-24-3p, miR-675- 5p, miR-486-3p, miR-6777-5p, miR-4497, miR-296-3p, miR-6738-5p, miR-4731-5p, The kit according to claim 1, further comprising a nucleic acid probe capable of specifically binding to at least one polynucleotide selected from the group consisting of miR-6889-5p, miR-6786-5p, miR-92a-3p, miR-4294, miR-4763-3p, miR-6076, miR-663a, miR-760, miR-4667-5p, miR-6090, miR-4730, miR-7106-5p, miR-3196, miR-5698, miR-6087, miR-4665-5p, miR-8059, and miR-6879-5p, and / or a primer that specifically recognizes and amplifies the polynucleotide.

3. A device for detecting esophageal cancer, comprising a nucleic acid probe capable of specifically binding to a polynucleotide of miR-6784-5p, which is an esophageal cancer marker and consists of the base sequence represented by SEQ ID NO: 33, and / or a primer that specifically recognizes and amplifies the polynucleotide.

4. The device is another esophageal cancer marker, consisting of the base sequences represented by SEQ ID NOs: 2 to 3, 5 to 7, 9 to 32, 35 to 214, and 666 to 676, namely miR-1247-3p, miR-6875-5p, miR-6726-5p, miR-3188, miR-8069, miR-1343-3p, miR-7108-5p, miR-6825-5p, miR-7641, miR-3185, miR-4746-3p, miR-6791-5p, miR-6893-5p, miR-4433b-3p, miR-3135b, miR-6781-5p, miR-1908-5p, miR-4792, miR-7845-5p, miR-4417, miR-3184-5p, miR-1225-5p, miR-1231, miR-1225-3p, miR-150-3p, miR-4433-3p, miR-6125, miR-4513, miR-6787-5p, miR-6765-3p, miR-5572, miR-6842-5p, miR-8063, miR-6780b-5p, miR-187-5p, miR-128-1-5p, miR-6729-5p, miR-6741-5p, miR-6757-5p, miR-7110-5p, miR-7975, miR-1233-5p, miR-6845-5p, miR-3937, miR-4467, miR-7109-5p, miR-6088, miR-6782-5p, miR-5195-3p, miR-4454, miR-6724-5p, miR-8072, miR-4516, miR-6756-5p, miR-4665-3p, miR-6826-5p, miR-6820-5p, miR-6887-5p, miR-3679-5p, miR-7847-3p, miR-6721-5p, miR-3622a-5p, miR-939-5p, miR-602, miR-7977, miR-6749-5p, miR-1914-3p, miR-4651, miR-4695-5p, miR-6848-5p, miR-1228-3p, miR-642b-3p, miR-6746-5p, miR-3620-5p, miR-3131, miR-6732-5p, miR-7113-3p, miR-23a-3p, miR-3154, miR-4723-5p, miR-3663-3p, miR-4734, miR-6816-5pmiR-4442、miR-4476、miR-423-5p、miR-1249、miR-6515-3p、miR-887-3p、m iR-4741、miR-6766-3p、miR-4673、miR-6779-5p、miR-4706、miR-1268b、mi R-4632-5p、miR-3197、miR-6798-5p、miR-6840-3p、miR-6763-5p 、miR-6727-5p、miR-371a-5p、miR-6824-5p、miR- 564、miR-3679-3p、miR-2861、miR-6737-5p、miR-4725-3p、miR-6716-5p、m iR-4675、miR-1915-3p、miR-3656、miR-6722-3p、miR-4707-5 p、miR-4449、miR-1202、miR-4649-5p、miR-744-5p、miR-642a-3p、miR-451 a、miR-6870-5p、miR-443、miR-6808-5p、miR-4728-5p、miR-937-5p、miR-1 35a-3p、miR-663b、miR-1343-5p、miR-6822-5p、miR-6803-5p、miR-6805-3 p、miR-128-2-5p、miR-4640-5p、miR-1469、miR-92a-2-5p、miR-3940-5p、m iR-4281、miR-1260b、miR-4758-5p、miR-5001-5p、miR-4286 、miR-6126、miR-6789-5p、miR-4459、miR-1268a、miR-6752-5p、miR-6131、 miR-6800-5p、miR-4532、miR-6872-3p、miR-718、miR-6769a-5p、miR-4707 -3p、miR-6765-5p、miR-4739、miR-4525、miR-4270、miR-4534、miR-6785-5 p、miR-6850-5p、miR-4697-5p、miR-1260a、miR-4486、miR-6880-5p、miR-6 802-5p、miR-6861-5p、miR-92b-5p、miR-1238-5p、miR-6851-5p、miR-7704、miR-149-3p, miR-4689, miR-4688, miR-125a-3p, miR-23b-3p, miR-614, miR-1913, m iR-16-5p, miR-6717-5p, miR-3648, miR-3162-5p, miR-1909-3p, miR-8073, miR-6769 b-5p, miR-6836-3p, miR-4484, miR-6819-5p, miR-6794-5p, miR-575, miR-24-3p, miR -675-5p, miR-486-3p, miR-6777-5p, miR-4497, miR-296-3p, miR-6738-5p, miR-4731 The device according to claim 3, further comprising a nucleic acid probe capable of specifically binding to at least one polynucleotide selected from the group consisting of miR-5698, miR-6087, miR-4665-5p, miR-8059, and miR-6879-5p, and / or a primer that specifically recognizes and amplifies the polynucleotide.

5. The device according to claim 3 or 4, wherein the device is a device for measurement by hybridization technology.

6. The device of claim 5 , wherein the hybridization technology is a nucleic acid array technology.

7. A method for assisting in the detection of esophageal cancer, comprising measuring the expression level of miR-6784-5p in a specimen from a subject using the kit according to claim 1 or the device according to claim 3 or claim 5 or 6 dependent on claim 3, comparing the measured expression level of the subject with a control expression level of a similarly measured healthy subject, and assessing in vitro that the subject is suffering from esophageal cancer when the expression level of the subject is higher than the expression level of the healthy subject.

8. The method of claim 7, wherein the subject is a human.

9. The method according to claim 7 or 8, wherein the sample is blood, serum or plasma.

10. Use of miR-6784-5p consisting of the base sequence represented by SEQ ID NO: 33 as an esophageal cancer marker for detecting esophageal cancer, The expression level of miR-6784-5p in a specimen from a subject is measured, and the measured expression level is compared with a control expression level from a similarly measured healthy subject. If the expression level in the subject is higher than the control expression level, the subject is assessed as having esophageal cancer. The above use.

11. The miR-6784-5p consists of the base sequences represented by SEQ ID NOs: 2-3, 5-7, 9-32, 35-214, and 666-676, respectively, and is miR-1247-3p, miR-6875-5p, miR-6726-5p, miR-3188, miR-8069, miR-1343-3p, miR-7108-5p, miR-6825-5p, miR-7641, miR-3185, miR-4746-3p, miR-6791-5p, miR-6893-5p, miR-4433b-3p, miR-3135b, miR-6781-5p, miR-1908-5p, miR-4792, miR-7845-5p, miR-4417, miR-3184-5p, miR-1225-5p, miR-1231, miR-1225-3p, miR-150-3p, miR-4433-3p, miR-6125, miR-4513, miR-6787-5p, miR-6765-3p, miR-5572, miR-6842-5p, miR-8063, miR-6780b-5p, miR-187-5p, miR-128-1-5p, miR-6729-5p, miR-6741-5p, miR-6757-5p, miR-7110-5p, miR-7975, miR-1233-5p, miR-6845-5p, miR-3937, miR-4467, miR-7109-5p, miR-6088, miR-6782-5p, miR-5195-3p, miR-4454, miR-6724-5p, miR-8072, miR-4516, miR-6756-5p, miR-4665-3p, miR-6826-5p, miR-6820-5p, miR-6887-5p, miR-3679-5p, miR-7847-3p, miR-6721-5p, miR-3622a-5p, miR-939-5p, miR-602, miR-7977, miR-6749-5p, miR-1914-3p, miR-4651, miR-4695-5p, miR-6848-5p, miR-1228-3p, miR-642b-3p, miR-6746-5p, miR-3620-5p, miR-3131, miR-6732-5p, miR-7113-3p, miR-23a-3p, miR-3154, miR-4723-5p, miR-3663-3p, miR-4734, miR-6816-5p, miR-4442miR-4476、miR-423-5p、miR-1249、miR-6515-3p、miR-4741、m iR-6766-3p、miR-4673、miR-6779-5p、miR-4706、miR-1268b、miR-4632-5p 、miR-3197、miR-6798-5p、miR-711、miR-6840-3p、miR-6763-5p、miR-6727 -5p、miR-371a-5p、miR-6824-5p、miR-4648、miR-1227-5p、miR-564、miR-36 79-3p、miR-2861、miR-6737-5p、miR-4675、mi R-1915-3p、miR-671-5p、miR-3656、miR-6722-3p、miR-4707-5p、miR-449 、miR-1202、miR-4649-5p、miR-744-5p、miR-642a-3p、miR-451a、miR-6870 -5p、miR-443、miR-6808-5p、miR-4728-5p、miR-937-5p、miR-135a-3p、miR -663b、miR-1343-5p、miR-6822-5p、miR-6803-5p、miR-128- 2-5p、miR-4640-5p、miR-1469、miR-92a-2-5p、miR-3940-5p、miR-4281、mi R-1260b、miR-4758-5p、miR-1915-5p、miR-4286、miR-6126、 miR-6789-5p、miR-4459、miR-1268a、miR-6752-5p、miR-6131、miR-6800-5p 、miR-4532、miR-6872-3p、miR-718、miR-6769a-5p、miR-4707-3p、miR-676 5-5p、miR-4739、miR-4525、miR-4270、miR-4534、miR-6785-5p、miR-6850- 5p、miR-4697-5p、miR-1260a、miR-4486、miR-680-5p、miR- 6861-5p、miR-92b-5p、miR-1238-5p、miR-6851-5p、miR-7704、miR-149-3p、miR-4689, miR-4688, miR-125a-3p, miR-23b-3p, miR-614, miR-1913, miR-16-5p , miR-6717-5p, miR-3648, miR-3162-5p, miR-1909-3p, miR-8073, miR-6769b-5p , miR-6836-3p, miR-4484, miR-6819-5p, miR-6794-5p, miR-575, miR-24-3p, miR -675-5p, miR-486-3p, miR-6777-5p, miR-4497, miR-296-3p, miR-6738-5p, miR- The use according to claim 10, wherein the polynucleotide is used as an esophageal cancer marker for detecting esophageal cancer in combination with at least one polynucleotide selected from the group consisting of miR-4731-5p, miR-6889-5p, miR-6786-5p, miR-92a-3p, miR-4294, miR-4763-3p, miR-6076, miR-663a, miR-760, miR-4667-5p, miR-6090, miR-4730, miR-7106-5p, miR-3196, miR-5698, miR-6087, miR-4665-5p, miR-8059, and miR-6879-5p.

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