Method for preparing composition comprising phytic acid from rice-bran

Abstract

To provide a method for preparing a composition comprising phytic acid from rice bran.SOLUTION: According to the present application, a composition comprising phytic acid can be efficiently produced from rice bran without requiring a degreasing process and without using a strong acid such as hydrochloric acid or sulfuric acid. The composition comprising phytic acid produced according to the present application has a low inorganic ion concentration, high antioxidant activity, and antimicrobial activity against fermenting microorganisms, and therefore can be used for the control of fermenting microorganisms.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present application relates to a method for producing a composition containing phytic acid from rice bran. [Background technology]

[0002] Rice bran is a fine grain separated from rice during the milling process from brown rice to polished rice after the husk has been removed. Rice bran may contain the pericarp, seed coat, aleurone layer, and part of the embryo, and is in the form of a crushed material.

[0003] The conventional technique for separating phytic acid from rice bran involves treating rice bran with an acid such as hydrochloric acid or sulfuric acid to obtain an extract, and then adding sodium hydroxide to the extract to neutralize it, thereby obtaining a precipitate containing phytic acid (Korean Patent Registration No. 10-0471558).

[0004] In this way, in the method of extracting phytic acid from rice bran using acid, a degreasing step is necessary to remove the oil from the rice bran because the raw material rice bran contains a large amount of oil.If phytic acid is produced without the degreasing step, it will act as an impurity in the refining process, making it difficult to purify to a high purity.

[0005] In addition, the precipitate containing phytic acid obtained by neutralizing rice bran after extraction with acid contains a large amount of protein. This is because a large amount of protein is incorporated into the phytic acid precipitate during the neutralization process, and the oil present in the rice bran also precipitates due to the interaction with the protein. If the phytic acid precipitate contains proteins that are difficult to separate, it becomes difficult to remove the oil and protein in the ion exchange process, which is a refining process. The remaining proteins and oils reduce the quality of the final phytic acid and reduce its shelf life.

[0006] Due to these drawbacks of the conventional techniques, it is necessary to develop a new method for efficiently separating phytic acid from rice bran using enzymes without using hydrochloric acid or sulfuric acid. [Prior art documents] [Patent documents]

[0007] [Patent Document 1] Korean Patent No. 10-0471558 Summary of the Invention [Problem to be solved by the invention]

[0008] The present inventors have conducted research and development to develop a method for producing a composition containing phytic acid from rice bran without the need for a rice bran degreasing process or a strong acid treatment process such as hydrochloric acid or sulfuric acid. As a result, they have experimentally confirmed that by treating rice bran with an enzyme having polysaccharide-degrading activity, proteolytic activity, or lipolytic activity, followed by filtration, neutralization, and ion exchange resin treatment of the enzyme-treated product, a phytic acid-containing composition with low inorganic ion content and high antioxidant and antibacterial activity can be produced without the need for a treatment process with an acid such as hydrochloric acid or sulfuric acid.

[0009] Therefore, an object of the present application is to provide a method for producing a composition containing phytic acid from rice bran without the steps of degreasing and acid treatment.

[0010] Another object of the present application is to provide a phytic acid-containing composition that has low concentrations of inorganic ions and high antioxidant activity.

[0011] It is yet another object of the present application to provide a phytic acid-containing composition that has antimicrobial activity against fermentation microorganisms. [Means for solving the problem]

[0012] In order to achieve the above objectives, One aspect of the present application provides a method for producing a composition containing phytic acid from rice bran, comprising the steps of: (a) treating rice bran with an enzyme to produce an enzyme extract of rice bran; (b) neutralizing the rice bran enzyme extract to produce a precipitate; (c) redissolving the precipitate to produce a redissolved product; and (d) contacting the redissolved material with an ion exchange resin.

[0013] Another aspect of the present application is a method for producing a phytic acid-containing food product, the method comprising: + , N.H. 4+ , K. + , Ca 2+ , Mg 2+ , Cl - , PO4 3- , and SO4 2- The present invention provides a composition containing phytic acid, in which the total concentration of the above ions is greater than 0 ppm and 400 ppm or less.

[0014] Yet another aspect of the present application is an antibacterial composition comprising a composition containing phytic acid as an active ingredient, (i) The composition containing phytic acid has a phytin content of 35% by weight or more based on the phytic acid-containing composition, and Na + , N.H. 4+ , K. + , Ca 2+ , Mg 2+ , Cl - , PO4 3- , and SO4 2- The total concentration of ions is greater than 0 ppm and less than 400 ppm. (ii) To provide an antibacterial composition having antibacterial activity against Bacillus subtilis.

[0015] The present application will be described in detail below.

[0016] In one aspect of the present application, there is provided a method for producing a composition containing phytic acid from rice bran, comprising the following steps (a) to (d):

[0017] Step (a): Treating rice bran with an enzyme to produce an enzyme extract of rice bran

[0018] In the present application, rice bran is treated with an enzyme to obtain an enzyme extract of rice bran. In the present application, the term "enzyme extract of rice bran" means an enzyme-treated product of rice bran obtained by treating rice bran with an enzyme, and in this specification, "enzyme extract of rice bran" is used in the same sense as "enzyme-treated product of rice bran."

[0019] In the present application, the enzyme used to treat rice bran may be an enzyme having one or more of polysaccharolytic activity, proteolytic activity, and lipolytic activity.

[0020] Specifically, the enzyme may be an enzyme having polysaccharolytic activity, which may be xylanolytic activity. More specifically, the enzyme may have polysaccharolytic activity and proteolytic activity and / or lipolytic activity, and the enzyme may be a multi-enzyme complex having two or more of the above activities.

[0021] In one embodiment, the enzyme used to treat the rice bran in step (a) may be an enzyme having one or more, more specifically two or more, or three or more activities selected from the group consisting of beta-glucanase, endo-1,3(4)-beta-glucanase (endo-1,3(4)-), cellulase, hemicellulase, xylanase, beta-xylanase, endo-1-4-beta-xylanase, amylase, alpha-amylase, pullulanase, lipase, and protease activities.

[0022] Specifically, the enzyme of the present application may have xylanolytic activity, more specifically, xylanase, beta-xylanase, or endo-1-4-beta-xylanase activity, and may further have one or more activities selected from the group consisting of beta-glucanase, endo-1,3(4)-beta-glucanase (endo-1,3(4)-), cellulase, hemicellulase, amylase, alpha-amylase, pullulanase, lipase, and protease activity in addition to the xylanolytic activity.

[0023] In one embodiment, the enzyme used in the present application may be a commercially available enzyme, and non-limiting examples thereof may include one enzyme or a combination of two or more enzymes selected from the group consisting of the following enzymes: pentopan 500BG, Celluclast 1.5L, Ultraflo Max, Cellic® CTec2, Shearzyme plus, Viscoflow MG, Viscoferm, Ondea Pro, Ceremix 6X MG, ​​and Viscozyme L.

[0024] In a more specific embodiment, the enzyme used in the present application may be a commercially available enzyme, and may be, as a non-limiting example, one enzyme or a combination of two or more enzymes selected from the group consisting of the following enzymes: pentopan 500BG, Ceremix 6X MG, ​​Ultraflo Max, Ondea Pro, Shearzyme plus, and Viscozyme L.

[0025] In one embodiment, the enzyme treatment of rice bran may be carried out by contacting the rice bran with an enzyme under conditions that allow the enzyme to react with the rice bran. For example, the enzyme treatment may be carried out by preparing an enzyme treatment reaction solution containing rice bran and the enzyme.

[0026] The content of rice bran in the enzyme-treated reaction solution may be, for example, 1-30 wt%, 1-28 wt%, 1-27 wt%, 1-26 wt%, 2-28 wt%, 2-27 wt%, 2-26 wt%, 3-28 wt%, 3-27 wt%, 3-26 wt%, 5-25 wt%, 6-25 wt%, 7-25 wt%, 8-22 wt%, 8-20 wt%, 8-18 wt%, 8-16 wt%, 8-14 wt%, or 8-12 wt%, but is not limited thereto.

[0027] The amount of enzyme in the enzyme treatment reaction solution may be 0.1-1 wt %, specifically 0.1-0.9 wt %, 0.1-0.8 wt %, 0.1-0.7 wt %, 0.1-0.6 wt %, 0.2-0.9 wt %, 0.3-0.9 wt %, 0.4-0.9 wt %, 0.2-0.8 wt %, 0.2-0.7 wt %, 0.2-0.6 wt %, 0.3-0.8 wt %, 0.3-0.7 wt %, 0.3-0.6 wt %, 0.4-0.8 wt %, 0.4-0.7 wt %, or 0.4-0.6 wt %.

[0028] The pH of the enzyme treatment reaction solution may be set taking into consideration the optimal pH of the enzyme used, and may be, for example, 4.3-5.5, 4.3-5.4, 4.3-5.3, 4.4-5.5, 4.4-5.4, 4.4-5.3, 4.5-5.5, 4.5-5.4, 4.5-5.3, 4.6-5.5, 4.6-5.4, 4.6-5.3, 4.7-5.5, 4.7-5.4, 4.7-5.3, 4.7-5.2, 4.7-5.1, or 4.7-5.0.

[0029] The temperature during the enzyme treatment reaction may be set in consideration of the optimal reaction temperature of the enzyme used, and may be, for example, 35-55°C, 36-54°C, 37-53°C, 38-52°C, or 39-52°C.

[0030] The reaction time for the enzyme treatment reaction may be 0.1-16 hours, 0.5-16 hours, 1-16 hours, 1-15 hours, 1-14 hours, 1-13 hours, 1-12 hours, 1-11 hours, 1-10 hours, 1-9 hours, 1-8 hours, 1-7 hours, 1-6 hours, 1-5 hours, 0.1-4 hours, 0.5-4 hours, 1-4 hours, 1.2-3.8 hours, 1.4-3.6 hours, 1.6-3.4 hours, 1.8-3 hours, 1.8-2.8 hours, 1.8-2.6 hours, or 1.8-2.4 hours.

[0031] In one embodiment, the present application may further include a step of filtering the rice bran enzyme extract after step (a) and before step (b).

[0032] The filtration may be carried out by passing the enzyme extract through a sieve having an appropriate mesh range, which may be 10-500 mesh, 10-400 mesh, 10-300 mesh, 10-200 mesh, 10-100 mesh, 20-500 mesh, 50-400 mesh, 70-300 mesh, 80-200 mesh, 90-150 mesh, 90-120 mesh, or 100 mesh.

[0033] In another embodiment, a filter aid such as diatomite or acid clay may be used during the filtration, and preferably diatomaceous earth.

[0034] In another embodiment, the filtration may be performed using filter paper, and may further include a filtration process using, for example, Whatman filter paper.

[0035] Step (b): Neutralizing the rice bran enzyme extract to produce a precipitate

[0036] In the present application, the rice bran enzyme extract or the filtered rice bran enzyme extract is neutralized to obtain a precipitate.

[0037] In one embodiment, the rice bran enzyme extract may be neutralized by adjusting the pH of the rice bran enzyme extract to a range of 7 to 7.5.

[0038] In another embodiment, the neutralization of the rice bran enzyme extract by adjusting the pH may be performed by adding an acidity regulator to the rice bran enzyme extract, and the acidity regulator may be, for example, sodium bicarbonate (NaHCO), monobasic sodium phosphate (NaHPO), or dibasic sodium phosphate (NaHPO).

[0039] In other embodiments, the resulting precipitate may be dried before being used in the subsequent redissolution step.

[0040] Step (c): Redissolving the precipitate to prepare a redissolved product

[0041] The precipitate obtained in step (b) is redissolved.

[0042] In one embodiment, the re-dissolution of the precipitate may be performed by adding an acidic substance, and the acidic substance may be, for example, one or more selected from the group consisting of citric acid, lactic acid, malic acid, and tartaric acid, but is not limited thereto.

[0043] In one embodiment, an acidic solution containing the acidic substance may be added to the precipitate to re-dissolve the precipitate. The concentration of the acidic substance in the acidic solution is not particularly limited and may be, for example, in the range of 0.5-3%.

[0044] In a specific embodiment, the reconstituted material may be a liquid solution, and the reconstituted liquid solution may be further treated with activated carbon to remove pigments or foreign matter.

[0045] Step (d): Contacting the redissolved material with an ion exchange resin

[0046] The liquid redissolved product obtained in step (c) is contacted with an ion exchange resin to remove ions in the redissolved product solution.

[0047] In one embodiment, the ion exchange resin may be a cation exchange resin, an anion exchange resin, or a combination of all of these.

[0048] In one embodiment, the cation exchange resin of the present application may be a strong acid cation exchange resin of sulfonic acid type or a weak acid cation exchange resin having a carboxylic acid group as an exchange group.

[0049] In one embodiment, the anion exchange resin of the present application may be a strongly basic anion exchange resin having a quaternary ammonium group as a functional group or a weakly basic anion exchange resin having a primary, secondary, or tertiary ammonium group as a functional group.

[0050] In one embodiment, the composition prepared by the method for preparing a composition containing phytic acid from rice bran, which comprises steps (a) to (d), contains 35% or more by weight of phytic acid based on the total composition, and Na + , N.H. 4+ , K. + , Ca 2+ , Mg 2+ , Cl - , PO4 3- , and SO4 2- The composition may contain phytic acid, in which the total concentration of the above ions is more than 0 ppm and 400 ppm or less.

[0051] Specifically, in the composition containing phytic acid, the content of phytic acid may be 35-60% by weight based on the total composition weight, more specifically, 35-58% by weight, 35-57% by weight, 35-56% by weight, 35-55% by weight, 35-54% by weight, 35-53% by weight, 35-52% by weight, 38-58% by weight, 38-57% by weight, 38-56% by weight, 38-55% by weight, 38-54% by weight, 38-53% by weight, 38-52% by weight based on the total composition weight. %, 41-58% by weight, 41-57% by weight, 41-56% by weight, 41-55% by weight, 41-54% by weight, 41-53% by weight, 41-52% by weight, 44-58% by weight, 44-57% by weight, 44-56% by weight, 44-55% by weight, 44-54% by weight, 44-53% by weight, 44-52% by weight, 47-58% by weight, 47-57% by weight, 47-56% by weight, 47-55% by weight, 47-54% by weight, 47-53% by weight, or 47-52% by weight.

[0052] In the composition containing phytic acid, Na + , N.H. 4+ , K. + , Ca 2+ , Mg 2+ , Cl - , PO4 3- , and SO4 2- The total concentration of these ions may be greater than 0 ppm and less than or equal to 400 ppm, specifically, 10-400 ppm, 10-390 ppm, 10-380 ppm, 10-370 ppm, 10-360 ppm, 10-350 ppm, 10-345 ppm, 10-344 ppm, 30-400 ppm, 30-390 ppm, 30-380 ppm, 30-370 ppm, 30-360 ppm, 30-350 ppm, 30-345 ppm, 30-344 ppm, 50-400 ppm, 50-390 ppm, 50-380 ppm, 50-370 ppm, 50-360 ppm, 50-350 ppm, 50-345 ppm, or 50-344 ppm.

[0053] In another aspect of the present application, the phytic acid content is 35% or more by weight of the total composition, and Na + , N.H. 4+ , K. + , Ca2+ , Mg 2+ , Cl - , PO4 3- , and SO4 2- The present invention provides a composition containing phytic acid, in which the total concentration of the above ions is greater than 0 ppm and 400 ppm or less.

[0054] In one embodiment, the composition containing phytic acid may be prepared from an enzyme-treated rice bran.

[0055] In one embodiment, in the composition of the present application containing phytic acid, the content of phytic acid may be 35-60% by weight of the total composition, more specifically, 35-58%, 35-57%, 35-56%, 35-55%, 35-54%, 35-53%, 35-52%, 38-58%, 38-57%, 38-56%, 38-55%, 38-54%, 38-53%, 38-52% by weight of the total composition. %, 41-58%, 41-57%, 41-56%, 41-55%, 41-54%, 41-53%, 41-52%, 44-58%, 44-57%, 44-56%, 44-55%, 44-54%, 44-53%, 44-52%, 47-58%, 47-57%, 47-56%, 47-55%, 47-54%, 47-53%, or 47-52% by weight.

[0056] In one embodiment, the composition containing phytic acid contains Na + , N.H. 4+ , K. + , Ca 2+ , Mg 2+ , Cl - , PO4 3- , and SO4 2-The total concentration of these ions may be greater than 0 ppm and less than or equal to 400 ppm, specifically, 10-400 ppm, 10-390 ppm, 10-380 ppm, 10-370 ppm, 10-360 ppm, 10-350 ppm, 10-345 ppm, 10-344 ppm, 30-400 ppm, 30-390 ppm, 30-380 ppm, 30-370 ppm, 30-360 ppm, 30-350 ppm, 30-345 ppm, 30-344 ppm, 50-400 ppm, 50-390 ppm, 50-380 ppm, 50-370 ppm, 50-360 ppm, 50-350 ppm, 50-345 ppm, or 50-344 ppm.

[0057] The phytic acid-containing compositions of the present application may be used as antioxidant compositions.

[0058] The phytic acid-containing compositions of the present application have the property of having a low degree of browning.

[0059] The low browning property means that the phytic acid-containing composition has excellent transparency maintaining properties during storage, and when such a composition is used as a food additive, it can minimize undesirable color changes.

[0060] In one embodiment, the degree of browning can be evaluated by measuring the absorbance of a composition containing phytic acid.

[0061] In one embodiment, the browning degree of the composition containing phytic acid may be expressed as the absorbance of the composition measured at 420 nm using a spectrophotometer.

[0062] In one embodiment, the composition containing phytic acid may have an absorbance value of 0.035-0.15, more specifically, 0.035-0.15, 0.037-0.15, 0.04-0.15, 0.04-0.14, 0.04-0.13, 0.04-0.12, 0.04-0.11, 0.045-0.15, 0.045-0.14, 0.045-0.13, 0.045-0.12, or 0.045-0.11, measured at 420 nm using a spectrophotometer. In this case, the absorbance value may be measured immediately after the preparation of the composition containing phytic acid.

[0063] In another specific example, the phytic acid-containing composition may have an absorbance value of 0.09-0.19, more specifically, 0.09-0.19, 0.1-0.19, 0.1-0.18, 0.11-0.18, 0.11-0.17, 0.11-0.16, 0.11-0.15, 0.11-0.14, or 0.11-0.13, measured at 420 nm using a spectrophotometer on day 0 of the storage period. In this case, the phytic acid-containing composition whose absorbance is measured may be concentrated to a soluble solids content of 40-41 Brix.

[0064] In another embodiment, the phytic acid-containing composition may have an absorbance value of 0.09-0.19, more specifically, 0.09-0.19, 0.1-0.19, 0.1-0.18, 0.11-0.18, 0.11-0.17, 0.11-0.16, 0.11-0.15, 0.11-0.14, or 0.11-0.13, measured at 420 nm using a spectrophotometer after storage at 10° C. for 15 days. In this case, the phytic acid-containing composition whose absorbance is measured may be concentrated to a soluble solids content of 40-41 Brix.

[0065] In another embodiment, the phytic acid-containing composition may have an absorbance value of 0.09-0.19, more specifically, 0.09-0.19, 0.1-0.19, 0.1-0.18, 0.11-0.18, 0.11-0.17, 0.11-0.16, 0.11-0.15, 0.11-0.14, or 0.11-0.13, measured at 420 nm using a spectrophotometer after storage at 10° C. for 30 days. In this case, the phytic acid-containing composition whose absorbance is measured may be concentrated to a soluble solids content of 40-41 Brix.

[0066] In another embodiment, the phytic acid-containing composition may have an absorbance value of 0.09-0.19, more specifically, 0.09-0.19, 0.1-0.19, 0.1-0.18, 0.11-0.18, 0.11-0.17, 0.11-0.16, 0.11-0.15, 0.11-0.14, or 0.11-0.13, measured at 420 nm using a spectrophotometer after storage at 10° C. for 45 days. In this case, the phytic acid-containing composition whose absorbance is measured may be concentrated to a soluble solids content of 40-41 Brix.

[0067] In another embodiment, the phytic acid-containing composition may have an absorbance value of 0.09-0.19, more specifically, 0.09-0.19, 0.1-0.19, 0.1-0.18, 0.11-0.18, 0.11-0.17, 0.11-0.16, 0.11-0.15, 0.11-0.14, or 0.11-0.14, measured at 420 nm using a spectrophotometer after storage at 10° C. for 60 days. In this case, the phytic acid-containing composition whose absorbance is measured may be concentrated to a soluble solids content of 40-41 Brix.

[0068] In another embodiment, the phytic acid-containing composition may have an absorbance value of 0.09-0.19, more specifically, 0.09-0.19, 0.1-0.19, 0.1-0.18, 0.11-0.18, 0.11-0.17, 0.11-0.16, 0.11-0.15, 0.11-0.14, 0.11-0.137, or 0.12-0.137, measured at 420 nm using a spectrophotometer after storage at 10° C. for 75 days. In this case, the phytic acid-containing composition whose absorbance is measured may be concentrated to a soluble solids content of 40-41 Brix.

[0069] In another embodiment, the phytic acid-containing composition may be stored at 10° C. for 0 to 75 days and the change in absorbance measured at 420 nm using a spectrophotometer between days 0 and 75 may be 0.015-0.008, 0.014-0.008, 0.013-0.008, 0.012-0.008, 0.011-0.008, 0.01-0.008, 0.0095-0.008, or 0.0095-0.0085. In this case, the change in absorbance is calculated by subtracting the absorbance measured on day 0 from the absorbance measured on day 75. The phytic acid-containing composition whose absorbance is measured may be concentrated to a soluble solids content of 40-41 Brix.

[0070] In a more specific embodiment, the absorbance may be measured using a spectrophotometer U-2900 (HITACHI, Co., Japan).

[0071] In another embodiment, the composition containing phytic acid may be prepared by the method for preparing a composition containing phytic acid from rice bran, which includes steps (a) to (d) described above.

[0072] Regarding the composition containing phytic acid of the present application, the content relating to the method for producing a composition containing phytic acid from rice bran is incorporated by reference into the content of one aspect of the present application already described, and will not be described again.

[0073] In another aspect of the present application, there is provided an antibacterial composition comprising a composition containing phytic acid as an active ingredient, the composition comprising phytic acid comprising phytic acid having a phytic acid content of 35% by weight or more based on the phytic acid-containing composition, and + , N.H. 4+ , K. + , Ca 2+ , Mg 2+ , Cl - , PO4 3- , and SO4 2- and (ii) the total concentration of these ions is more than 0 ppm and 400 ppm or less, and the antibacterial composition has antibacterial activity against Bacillus subtilis.

[0074] In one embodiment, the antibacterial composition containing phytic acid may be prepared from an enzyme-treated rice bran.

[0075] In one embodiment, the antibacterial composition containing phytic acid may be prepared by the method for preparing a composition containing phytic acid from rice bran, which includes steps (a) to (d) described above.

[0076] In one embodiment, the composition containing phytic acid, which is an active ingredient of the antibacterial composition, is the same as the composition containing phytic acid in another aspect of the present application described above. Therefore, the content of this composition is incorporated by reference and will not be described again.

[0077] In the antibacterial composition of the present application, the content relating to the method for producing a composition containing phytic acid from rice bran is incorporated by reference in the content of one aspect of the present application already described, and will not be described again.

[0078] The composition containing phytic acid of the present application has antioxidant activity and antibacterial activity against fermentation microorganisms, particularly against Bacillus subtilis, and therefore may be used as a food additive to be added to various foods in need of such activity.

[0079] The composition containing phytic acid of the present application may be applied to foods, feeds, daily necessities, industrial products, etc. without limitation. Specific examples of foods or feeds include processed grain products, vegetables, fruits, dried and cut vegetable products, fruit juice, vegetable juice, mixed vegetable and fruit juice, chips, noodles, processed livestock foods, processed seafood foods, processed dairy foods, fermented dairy foods, microbially fermented foods, confectionery and bread, seasonings, processed fish / meat products, acidic drinks, processed foods, convenience foods, licorice, herbs, insect feed, livestock feed, and pet feed, but are not limited thereto.

[0080] The fish / meat processed products refer to hams, sausages, bacons, dried and preserved meats, seasoned meats, packaged meats, crushed processed meat products, processed ribs, meat extracts, edible beef tallow, edible pork fat, chunks of fish meat, etc., which are processed using meat or fish meat as a raw material.

[0081] The "meat" may include, but is not limited to, meats commonly used in dietary habits, such as beef, pork, lamb, goat, rabbit, chicken, turkey, duck, pheasant, and quail, as well as edible organs and by-products. Forms of processed meat include, but are not limited to, sterilized meat products, hams, pressed hams, mixed pressed hams, sausages, mixed sausages, dried sausages (dried mixed sausages), semi-dried sausages (semi-dried mixed sausages), cooked and frozen sausages, bacon, dried and preserved meats, seasoned meats, ground processed products, processed ribs, packaged meats, and other processed meat products.

[0082] When the composition containing phytic acid of the present application is used according to its intended purpose, it may be formulated into various forms such as liquid, solid, powder, etc. in a convenient and suitable manner according to its intended purpose.

[0083] The compositions of the present application may be blended with ingredients during the manufacturing process, and the food product may be immersed in the composition of the present application, immersed and then stirred, sprayed with the composition, or directly mixed with the food product so that it is evenly applied to the food product. [Effects of the Invention]

[0084] According to the present application, a composition containing phytic acid can be efficiently produced from rice bran without the need for a rice bran degreasing step and without the use of strong acids such as hydrochloric acid or sulfuric acid.

[0085] The phytic acid-containing composition produced by the present application has a low concentration of inorganic ions, high antioxidant activity, and antibacterial activity against fermentation microorganisms, and can therefore be used to control fermentation microorganisms.

[0086] However, the effects of the present application are not limited to the effects mentioned above, and other effects not mentioned will be clearly understood by those skilled in the art from the following description. [Brief explanation of the drawings]

[0087] [Figure 1] 1 is a comparative photograph of the phytic acid-containing solution of the present application in Production Example 2, other company's product 1 (sulfuric acid treatment method), and other company's product 2 (hydrochloric acid treatment method). [Figure 2] 1 shows the results of measuring the antibacterial effect of the rice bran extract of the present application against fermentation microorganisms using a disk diffusion method. DETAILED DESCRIPTION OF THE INVENTION

[0088] The present application will be described in more detail below with reference to examples. However, the following examples are provided to specifically illustrate the present application, and the contents of the present application are not limited to the following examples. [Example]

[0089] [Production Example 1] Production of enzyme extract from rice bran

[0090] 1. Enzyme treatment of rice bran The rice bran was purchased after polishing and stored in a refrigerator, and was then frozen to prevent deterioration in quality before being used as an experimental material.

[0091] An enzyme-treated rice bran extract was prepared by treating rice bran with enzymes (Novozyme A / S) that have beta-glucanase, cellulase, xylanase, beta-xylanase, amylase, alpha-amylase, pullulanase, and protease activity. The enzymes used in the experiment are listed in Table 1 below.

[0092] [Table 1]

[0093] To treat rice bran with enzymes, a rice bran enzyme treatment reaction solution containing enzymes, rice bran, and purified water was prepared. The pH range and temperature of the enzyme were determined based on the enzyme's active pH range and active temperature provided by the manufacturer (Novozyme A / S). The pH of the enzyme treatment reaction solution was adjusted by adding 0.3% citric acid. Based on the total enzyme treatment reaction solution, 10% by weight of rice bran and 0.5% by weight of enzyme were used, and the treatment time was fixed at 13-14 hours. The reaction conditions for each enzyme treatment reaction are listed in Table 2 below.

[0094] [Table 2]

[0095] 2. Solid content measurement The soluble solids content of the rice bran enzyme-treated extract (hereinafter referred to as "rice bran enzyme extract" or "rice bran extract") obtained by treating rice bran with an enzyme was measured using an ATAGO RX-5000α saccharometer. The solids content of the rice bran extracts obtained using the enzymes used is shown in Table 3 below.

[0096] [Table 3]

[0097] Among the enzyme characteristics, the group treated with a high enzyme titer, which has xylanase properties that decompose xylan, showed a higher solids content. The group treated with an enzyme with multiple activities showed an even higher solids content than the group treated with an enzyme with a single activity.

[0098] 3. Measurement of total polyphenol content and DPPH radical scavenging activity Total polyphenol content was measured using the Folin-Ciocalteu method. 0.1 ml of sample diluted to a certain concentration in a microtube was mixed with 0.05 ml of 50% Folin-Ciocalteu reagent and incubated at room temperature for 3 minutes. After incubation, 0.15 ml of 2% Na2CO3 solution was added and incubated at room temperature for an additional 30 minutes. Absorbance was measured at 700 nm using a microplate reader (M2, Molecular Device, Canada). Gallic acid was used as the standard stock, and a standard calibration curve was prepared at 50, 60, 70, 80, and 100 mg / L.

[0099] DPPH radical scavenging activity was measured using the Blois method. A 4 mM ethanol solution of DPPH (1,1-diphenyl-2-picrylhydrazyl, Sigma Chemical Co.) was prepared and used after adjusting the absorbance to 1.000 ± 0.1. Specifically, 0.2 mL of each sample was added to a test tube, mixed with 2.8 mL of DPPH solution, and incubated for 10 minutes. The activity was then measured at 517 nm using a microplate reader (M2, Molecular Device, Canada). DPPH radical scavenging activity was calculated using the following formula: DPPH radical scavenging activity(%)=[1-(A / B)]X100 (A: absorbance of the sample-added group, B: absorbance of the sample-free group)

[0100] The measurement results of the total polyphenol content and DPPH radical scavenging activity of rice bran extract are shown in Table 4 below.

[0101] [Table 4]

[0102] As the enzymes decomposed the cellulose, many functional components present in the cell walls were extracted, including polyphenols, indicating a high total polyphenol content. The enzyme with the highest measured polyphenol content was Ceremix 6x MG, followed by Ultraflo Max, which also showed high levels. Furthermore, DPPH radical scavenging activity, which indicates antioxidant effects, was high at 94.14-90.46%, indicating that it was not proportional to the total polyphenol content.

[0103] 4. Analysis of phenolic compounds To identify the polyphenolic components in the rice bran enzyme extract, phenolic compounds were analyzed using HPLC (Agilent Technologies, 1260 Infinity II, USA). A 20 μL sample of rice bran extract was used. The column was an Eclipse XDB-C18 HPLC column (4.6 × 250 mm, 5 μm) and the detector was a DAD (Agilent Technologies, 1260 Infinity II, USA) at 280 nm. The mobile phase consisted of a 30:70 mixture of methanol (A) and 50 mM sodium hydrogen phosphate (NaHPO / pH 2.5, phosphoric acid) (B). The mobile phase flow rate was 1.0 mL / min, and the column temperature was 40°C for 30 minutes. Standards were prepared at concentrations of 1, 3, 5, 6, 8, and 10 mg / L to create a standard calibration curve. The analysis results for phenolic compounds are shown in Table 5.

[0104] [Table 5]

[0105] 5. Measurement of Phytic Acid Content The phytic acid content in rice bran extract was measured. The indicator used for phytic acid measurement was 300 mg sulfosalicylic acid and 30 mg ferric chloride hexahydrate (Wade reagent) in 100 mL of distilled water, stored at 2–4°C. Specifically, the sample was diluted, and 3 mL of the dilution solution and 1 mL of Wade reagent were mixed in a 15 mL conical tube. The mixture was centrifuged at 3,000 rpm and 10°C for 10 minutes, and the absorbance was measured at 500 nm. A 100 mg / L standard stock was prepared using phytic acid sodium salt hydrate obtained from rice, and a standard calibration curve was prepared at 0, 20, 40, 60, 80, and 100 mg / L. The phytic acid content of the rice bran extract measured using the enzyme is shown in Table 6 below.

[0106] [Table 6]

[0107] Rice bran contains phytic acid and magnesium 2+ , K. + , Ca 2+ It is known that phytic acid exists in the form of a salt mixture bound to ions such as phytate. It has been shown that rice bran extracts extracted by enzyme treatment contain phytic acid in the form of crude phytate at a content of 0.24-0.97% by weight. This value corresponds to a phytic acid content of 2.14-8.76% by weight when converted to 100 g of enzyme-treated raw rice bran. [Example]

[0108] [Production Example 2] Production of phytic acid-containing solution

[0109] A solution containing phytic acid was prepared using the enzyme extracted with a high phytic acid content in Preparation Example 1 and the enzyme confirmed to have a high antioxidant effect. After treating rice bran with the enzyme, the solution was filtered, neutralized, precipitated, redissolved, purified, and concentrated to prepare a phytic acid-containing solution.

[0110] 1. Enzyme treatment The enzyme treatment was carried out under the conditions shown in Table 7 below. The enzyme treatment time was varied between 1, 2, and 4 hours, and then inactivated at 90°C for 10 minutes.

[0111] [Table 7]

[0112] 2. Measurement of phytic acid content by enzyme treatment time The phytic acid content of the enzyme extract obtained by enzymatically treating rice bran was measured according to the enzyme treatment time (extraction time). The phytic acid content was measured using the same method as described in Preparation Example 1, and the measurement results are shown in Table 8 below.

[0113] [Table 8]

[0114] The change in phytic acid content as a function of extraction time was confirmed. The content was low at the beginning of extraction and increased with increasing extraction time. Most enzymes extracted high amounts of phytic acid (0.618-0.726%) after two hours of extraction, but Ceremix 6X MG extracted a low amount (0.397%), showing a tendency to decrease. After four hours of extraction, the phytic acid content decreased in all enzyme treatment groups. Therefore, when extracting rice bran using enzymes, it was confirmed that the extraction efficiency actually decreased with increasing extraction time.

[0115] 3. Separation of crude phytate solids and removal of metal ions Rice bran enzyme extract obtained by enzymatically treating rice bran was subjected to primary filtration using a 100 mesh standard. The filtered extract was subjected to secondary filtration using 5% by weight of diatomaceous earth (based on the total weight of the extract) as a filter aid and Whatman No. 2 filter paper placed in a Buchner funnel. The pH of the secondary filtered extract was neutralized to a range of 7.00-7.50 using NaHCO3. The neutralized extract was left to stand for 12 hours to induce precipitation. The precipitated extract was then filtered again using Whatman No. 2 filter paper placed in a Buchner funnel to separate the precipitate. The separated precipitate was completely dried in a dry oven at 60°C to separate crude phytate solids. Crude phytate is a salt form of phytic acid, which can be calcium, magnesium, zinc, or sodium salts, and its main components are calcium and magnesium salts of phytic acid.

[0116] The solid obtained by drying, in the form of crude phytate, was redissolved in a 1% citric acid solution to produce a liquid, which was then treated with activated carbon to remove pigments and impurities. Metal ions were then removed using a cation exchange resin, and the resulting solution was concentrated to 40 Brix based on the soluble solids in a vacuum concentrator to produce a phytic acid-containing solution. The cation exchange resin used to remove metal ions was TRILITE MC-08 (Samyang Co., Korea), and the specifications of the ion exchange resin are listed in Table 9 below.

[0117] [Table 9]

[0118] 4. Analysis of inorganic ion content The content of inorganic substances remaining in the phytic acid-containing solution purified using an ion exchange resin was confirmed by inorganic ion analysis.

[0119] Inorganic ion analysis was performed using a Metrohm MagIc Net system with a conductivity detector. The column used for anion analysis was a Metrosep A supp 5 column (Metrohm, 150 x 4 mm), with a mobile phase of 3.2 mM sodium carbonate and 1.0 mM sodium bicarbonate at a flow rate of 0.7 mL / min. The column used for cation analysis was a Metrosep C4 column (Metrohm, 150 x 4 mm), with a mobile phase of 0.7 mM dipicolinic acid and 1.7 mM nitric acid at a flow rate of 0.9 mL / min. The sample pretreatment method was to add 9.9 mL of distilled water to 0.1 mL of sample solution, mix thoroughly, and then centrifuge (10,000 rpm, 10 min, 4°C). The supernatant was filtered through a 0.25 μm syringe filter, and 10 μL was injected for analysis.

[0120] The analytical results of inorganic ions before purification with ion exchange resins are shown in Table 10 below, and the analytical results of inorganic ions after purification with ion exchange resins are shown in Table 11 below.

[0121] [Table 10]

[0122] [Table 11]

[0123] In general, metal ions are known to act as catalysts or to structurally contribute to the function of enzymes, enhancing their activity. The most common cation form is Na + , K. + , Ca 2+ , Mg 2+It is known that enzymes such as hydroxybenzoates and hydroxybenzoates act on rice bran extracts. In the case of rice bran extracts extracted using enzymes, some metal ions were used or not depending on the type and mechanism of action of the enzyme, which is thought to have been beneficial in terms of improving purification efficiency. The concentration of metal ions after purification with ion exchange resins was relatively reduced, and extraction methods using acids generally showed higher residual metal ion content than enzyme extraction methods. In addition, solutions extracted using acids as the main extraction solvent showed a higher residual metal ion rate. [Example]

[0124] [Experimental Example 1] Measurement of antioxidant capacity - Measurement of DPPH radical scavenging activity and browning degree

[0125] The phytic acid-containing solution produced in Production Example 2 was measured for DPPH radical scavenging activity and browning degree.

[0126] DPPH radical scavenging activity was measured using the Blois method. A 4 mM ethanol solution of DPPH (1,1-diphenyl-2-picrylhydrazyl, Sigma Chemical Co.) was prepared and used after adjusting the absorbance to 1.000 ± 0.1. Specifically, 0.2 mL of each sample was added to a test tube, and 2.8 mL of DPPH solution was added. After incubation for 10 minutes, the sample was measured at 517 nm using a microplate reader (M2, Molecular Device, Canada). DPPH radical scavenging activity was calculated using the following formula: DPPH radical scavenging activity(%)=[1-(A / B)]X100 (A: absorbance of the sample-added group, B: absorbance of the sample-free group)

[0127] To measure the degree of browning in the phytic acid-containing solution, the absorbance was measured at 420 nm using a spectrophotometer (U-2900, Hitachi Co., Japan) immediately after preparation without concentrating. The measurement results of DPPH radical scavenging activity and browning degree are shown in Table 12 below.

[0128] [Table 12]

[0129] As shown in Table 12, DPPH scavenging activity, which indicates antioxidant activity, was high for all enzymes used in extraction. Among the enzymes used, Ultraflo Max had the highest effect at 80.57%, while Shearzyme Plus had the lowest effect. Browning rates were similar across all enzymes. To examine changes in browning rates of phytic acid-containing solutions over storage periods, metal ions were removed from phytic acid-containing solutions, and Ultraflo Max-treated solutions were extracted with high phytic acid content. These solutions were concentrated to a concentration of 40-41 Brix and stored at 10°C. Absorbance (Abs) measurements were performed every 15 days to confirm changes in the solution color. Controls were also used, measuring the absorbance of phytic acid-containing solutions obtained using the hydrochloric acid and sulfuric acid extraction methods under the same conditions. The absorbance results for each solution are shown in Table 13.

[0130] [Table 13]

[0131] The browning degree of the phytic acid-containing solution obtained by enzyme extraction was examined and showed little change with storage time. Specifically, the absorbance of the phytic acid-containing solution obtained by enzyme extraction was 0.123 on day 0 and 0.132 on day 75, an increase of 0.009. The absorbance of the phytic acid-containing solution obtained by hydrochloric acid extraction was 0.195 on day 0 and 0.228 on day 75, an increase of 0.033. The absorbance of the phytic acid-containing solution obtained by sulfuric acid extraction was 0.285 on day 0 and 0.305 on day 75, an increase of 0.02. This confirmed that the browning degree (insignificant change in color) of the phytic acid-containing solution obtained by enzyme extraction was the lowest. [Example]

[0132] [Experimental Example 2] Measurement of phytic acid content, antioxidant capacity, and transparency

[0133] The phytic acid content of the phytic acid-containing solution produced in Production Example 2 was measured, and the phytic acid content of Competitor's Product 1 (sulfuric acid treatment method) and Competitor's Product 2 (hydrochloric acid treatment method), which were produced using sulfuric acid or hydrochloric acid, was also measured. The results are shown in Table 14 below.

[0134] In addition, the antioxidant capacity of the phytic acid-containing solution prepared in Preparation Example 2 and a competitor's product prepared using acid was measured using DPPH radical scavenging ability and ABTS analysis. The DPPH radical scavenging ability was measured using the same method as described in Experimental Example 1.

[0135] ABTS analysis was performed using a modified version of the method by Re et al. (Re R., N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, and C. Rice-Evans 1999, Free Radic Biol Med. 26:1231-1237.) The ABTS solution was prepared by mixing the sample with an ABTS solution containing 7.4 mM ABTS and 2.45 mM KSO. The mixture was incubated at room temperature for 6 minutes, and the absorbance was measured at 734 nm.

[0136] The measurement results of the antioxidant capacity of the phytic acid-containing solution and other companies' products are shown in Table 14 below. As shown by the measurement results, it can be confirmed that the antioxidant capacity of the phytic acid-containing solution of Preparation Example 2 of the present application is superior to that of other companies' products.

[0137] [Table 14]

[0138] 1 shows comparative photographs of the phytic acid-containing solution of Production Example 2, Competitor's Product 1 (treated with sulfuric acid), and Competitor's Product 2 (treated with hydrochloric acid). From Fig. 1, it can be seen that the phytic acid-containing solution of Production Example 2 of the present application had a lower degree of browning and better transparency than Competitor's Product 1 and Competitor's Product 2, which were both treated with acid.

[0139] [Experimental Example 3] Measurement of antibacterial activity

[0140] To determine whether the phytic acid-containing solution of Preparation Example 2 has inhibitory activity against fermentation microorganisms, its antibacterial effect against fermentation microorganisms was measured. Among the rice bran extracts extracted using enzymes, Ultraflo Max had the highest phytic acid content and antioxidant activity, so the antibacterial effect against fermentation microorganisms was determined. A rice bran extract prepared by acid treatment was used as a control.

[0141] 1. Strain culture The strains used in the experiment were Lactobacillus sakei KCTC3598 and Leuconostoc mesenteroides KCTC3505, lactic acid bacteria derived from kimchi, which were subcultured every 24 hours in MRS Broth at 35°C, and Bacillus subtilis KCTC1021, a microorganism derived from traditional sauce, which was subcultured every 24 hours in TSB at 35°C under anaerobic conditions.

[0142] 2. Paper disc diffusion method To measure the antibacterial activity of the samples, paper disc diffusion was performed to confirm the formation of growth inhibition rings for each strain. For Lactobacillus sakei KCTC3598 and Leuconostoc mesenteroides KCTC3505, MRS broth was adjusted to a turbidity of 0.5 McFarland standard and poured onto MRS agar medium. Once the medium solidified, an 8 mm diameter paper disc containing the sample solution was placed on top. After 24 hours of incubation at 35°C, the size of the growth inhibition ring formed was measured. For Bacillus subtilis KCTC1021, TS broth was adjusted to a turbidity of 0.5 McFarland standard and poured onto TS agar medium. When the medium hardened, an 8 mm diameter paper disc containing the sample solution was placed on it, and after anaerobic culture at 37°C for 48 hours, the size of the growth inhibition ring formed was measured.

[0143] 3.Minimum inhibitory concentration The minimum inhibitory concentration (MIC) is the minimum concentration of an antibiotic that inhibits the growth of a microorganism. The lower the MIC value for a microorganism, the more sensitive the sample substance is to that microorganism. MIC measurements were performed using a modified broth dilution method. 100uL of broth containing 2-fold diluted samples and 1.0x10 6 100 μL of bacteria adjusted to CFU / mL was added and cultured at 35°C for 24 hours to determine the minimum concentration at which bacterial growth was not observed.

[0144] 4. Minimum bacterial concentration The MIC is the minimum inhibitory concentration of bacteria, and does not mean that all bacteria have been killed. To determine the minimum lethal concentration (MBC), a sample culture solution above the MIC value was streaked onto a solid medium and cultured. After colony formation was confirmed, the concentration at which no bacteria grew was determined as the MBC value. The sample culture solution was smeared using a loop onto a 96-well plate where the MIC had been determined, and cultured at 35°C for 24 hours to determine the minimum lethal concentration.

[0145] 5. Antibacterial effect measurement results - disk diffusion method To confirm the antibacterial effect against fermentation microorganisms, the clear zone (mm), which is the growth inhibition zone of bacteria, was measured. The results are shown in Table 15 below and FIG.

[0146] [Table 15]

[0147] Lactobacillus sakei showed a growth inhibition ring of 1.05mm-1.31mm, showing no significant difference between extraction methods, while Leuconostoc mesenteroides showed a growth inhibition ring of 0.48mm-0.62mm, showing the greatest antibacterial effect with an extraction method using sulfuric acid (H2SO4).For Bacillus subtilis, the enzyme-based extraction method showed the best growth inhibition ring, indicating the greatest antibacterial effect.

[0148] 6. Antibacterial effect measurement results - minimum inhibitory concentration and minimum killing concentration The results of confirming the minimum inhibitory concentration and minimum killing concentration of rice bran extract are shown in Table 16 below.

[0149] [Table 16]

[0150] The concentrations of rice bran extracts exhibiting antibacterial effects showed similar trends for each extraction method, as demonstrated by disk diffusion analysis. For Lactobacillus sakei, the minimum inhibitory concentration (MINC) for all extraction methods was 625 μL / ml, and the minimum kill concentration increased to 1250 μL / ml, except for the hydrochloric acid (HCl) extraction method. For Leuconostoc mesenteroides, the minimum inhibitory concentration was 312 μL / ml for all extraction methods except the sulfuric acid (H2SO4) extraction method, but the minimum kill concentration increased to a higher concentration of 1250 μL / ml. For Bacillus subtilis, both the minimum inhibitory and minimum kill concentrations were 625 μL / ml, demonstrating the lowest concentration of any fermentation microorganism.

[0151] The results of the antibacterial experiments confirmed that the phytic acid-containing rice bran extract obtained by the enzyme treatment method exhibited antibacterial activity against fermentation microorganisms that was equal to or superior to that of the rice bran extract obtained by the acid treatment method. The phytic acid-containing rice bran extract obtained by the enzyme treatment method of the present application can be used as a regulator that can control the degree of fermentation by fermentation microorganisms by regulating the growth and death of fermentation microorganisms.

[0152] The above describes exemplary embodiments of the present application, but the scope of the present application is not limited to the specific embodiments described above, and a person having ordinary knowledge in the relevant field can make appropriate modifications within the scope of the claims of the present application.

Claims

1. The phytic acid content is 35% by weight or more based on the total composition; Na ＋ , NH4 ＋ , K. ＋ , Ca ２＋ , Mg ２＋ , Cl － , P.O. ４ ３－ , and SO ４ ２－ A composition containing phytic acid, wherein the total concentration of the above ions is greater than 0 ppm and less than 400 ppm.

2. The composition containing phytic acid according to claim 1, wherein the composition is produced from an enzyme extract obtained by enzymatically treating rice bran.

3. 2. The composition comprising phytic acid according to claim 1, wherein the composition has an absorbance of 0.035-0.15 at 420 nm measured using a spectrophotometer.

4. An antibacterial composition comprising a composition containing phytic acid as an active ingredient, (i) The composition containing phytic acid has a phytic acid content of 35% by weight or more based on the phytic acid-containing composition, and Na ＋ , NH4 ＋ , K. ＋ , Ca ２＋ , Mg ２＋ , Cl － , P.O. ４ ３－ , and SO ４ ２－ The total concentration of the ions is more than 0 ppm and 400 ppm or less, (ii) An antibacterial composition having antibacterial activity against Bacillus subtilis.

5. The antibacterial composition according to claim 4, wherein the composition containing phytic acid is produced from an enzyme extract obtained by enzymatically treating rice bran.

6. 5. The antibacterial composition according to claim 4, wherein the composition containing phytic acid has an absorbance of 0.035-0.15 at 420 nm measured using a spectrophotometer.

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