Polypeptides comprising immunoglobulin single variable domains targeting TNFα and ox40l

Abstract

To provide a novel type of drug for treating a subject suffering from an autoimmune or inflammatory disease.SOLUTION: The present technology provides polypeptides comprising at least four immunoglobulin single variable domains (ISVDs), characterized in that at least two ISVDs bind to TNFα and at least two ISVDs bind to OX40L. The present technology also provides nucleic acids, vectors and compositions.SELECTED DRAWING: None

Description

[Technical Field]

[0001] 1 Field of this technology The present technology relates to polypeptides that target TNFα and OX40L.The present technology also relates to nucleic acid molecules encoding the polypeptides and vectors containing the nucleic acids, as well as compositions containing the polypeptides, nucleic acids, or vectors.The present technology further relates to such products for use in methods for treating subjects suffering from autoimmune or inflammatory diseases.Furthermore, the present technology relates to methods for producing such products. [Background technology]

[0002] 2 Technical background Autoimmune or inflammatory diseases are the result of the body's immune response to its own tissues. Autoimmune or inflammatory diseases are often chronic and can even be life-threatening. Autoimmune or inflammatory diseases include inflammatory bowel diseases such as Crohn's disease and ulcerative colitis, rheumatoid arthritis, psoriasis, psoriatic arthritis, and hidradenitis suppurativa. Inflammatory bowel diseases such as Crohn's disease and ulcerative colitis are chronic inflammatory diseases accompanied by intestinal inflammation and associated epithelial damage. Other chronic autoimmune diseases such as psoriasis, psoriatic arthritis, and hidradenitis suppurativa are characterized by red, dry, itchy, or scaly skin, painful joint inflammation, or inflamed and swollen skin lumps. It has been found that patients with psoriasis are more likely to have certain comorbidities, including diabetes, inflammatory bowel diseases such as Crohn's disease or ulcerative colitis, and cancer.

[0003] Tumor necrosis factor α (TNFα) is a homotrimeric cytokine produced primarily by monocytes and macrophages, but not by CD4 + and CD8 +It is also known to be secreted by peripheral blood T lymphocytes. TNFα can exist as a soluble or transmembrane protein. The primary role of TNFα is to regulate immune cells. TNFα acts as an endogenous pyrogen, and dysregulation of its production has been implicated in various human diseases, including rheumatoid arthritis (RA), psoriasis (Pso), hidradenitis suppurativa (HS), inflammatory bowel diseases (IBD) such as Crohn's disease (CD) and ulcerative colitis (UC), and graft-versus-host disease (GVHD).

[0004] Currently FDA-approved treatments for RA and IBD inflammatory bowel disease include anti-TNFα biopharmaceuticals (Simponi® [golimumab], Enbrel® [etanercept], Remicade® [infliximab], Humira® [adalimumab], etc.). However, current anti-TNFα treatments for RA only result in complete disease remission in a minority of patients, and a significant proportion of non-responders remain. Similarly, current anti-TNFα treatments for inflammatory bowel disease face the non-responsiveness of a large proportion of patients to currently available treatments, with a high proportion of patients losing response to anti-TNFα treatment after 12 months of treatment. In the case of psoriasis and psoriatic arthritis, only a minority of patients are treated with biopharmaceuticals, including Remicade® [infliximab] and Humira® [adalimumab]. Current treatments have been shown to have some efficacy in treating psoriasis, at least in a subset of patients.

[0005] Thus, for example, in the case of autoimmune diseases such as rheumatoid arthritis or psoriatic arthritis, no biopharmaceutical to date has demonstrated sufficient efficacy in a significant proportion of patients in terms of disease remission, and lack or loss of response remains a problem.

[0006] OX40L (also known as CD252 or TNFSF4) is a member of the TNF superfamily and an inducible costimulatory ligand for the OX40 receptor (also known as CD134 or TNFRSF4). OX40L is primarily expressed on activated antigen-presenting cells (APCs), including dendritic cells, macrophages, and B cells. OX40, on the other hand, is predominantly expressed on activated T cells and natural killer T cells. OX40L is mostly expressed as a membrane-bound molecule, but it can also be detected in a cleaved, soluble form. OX40L / OX40 is recognized as an immune costimulatory regulator in numerous diseases characterized by activated T cells orchestrating the immune response. This triggers OX40-mediated signaling, resulting in a series of activities, including the production and release of proinflammatory cytokines, the expansion and accumulation of effector T cells (e.g., TH1, TH2, TH17) and cytotoxic T cells, and a decrease in the suppressive efficacy of Treg cells. Although several studies suggest that the costimulatory OX40L / OX40 axis is involved in autoimmune diseases such as RA, Pso, or IBD, there are currently no FDA-approved OX-40L biopharmaceuticals for their treatment.

[0007] Targeting multiple disease factors can be achieved, for example, by simultaneous administration or combined use of two separate biopharmaceuticals, such as antibodies that bind to different therapeutic targets.However, simultaneous administration or combined use of separate biopharmaceuticals can be difficult from both practical and commercial perspectives.For example, two separate product injections can make the treatment regimen more inconvenient and more painful for patients, which can negatively affect compliance.With regard to a single injection of two separate products, it can be difficult or impossible to provide a formulation that allows both products to have the required concentration and acceptable viscosity with suitable stability.In addition, simultaneous administration and simultaneous formulation require the production of two separate drugs, which can increase overall costs.

[0008] Bispecific antibodies, capable of binding to two different antigens, have been proposed as one strategy to address such limitations associated with the co-administration or combinatorial use of separate biopharmaceuticals such as antibodies.

[0009] Several formats of bispecific antibody constructs have been proposed. For example, a bispecific antibody format may involve chemical conjugation of two antibodies or their fragments (Non-Patent Document 1; Non-Patent Document 2).

[0010] However, disadvantages of such bispecific antibody formats include high viscosity at high concentrations, which makes subcutaneous administration difficult, and each binding unit requires the interaction of two variable domains for specific, high-affinity binding, which affects polypeptide stability and production efficiency. Such bispecific antibody formats may also be associated with chemistry, manufacturing, and quality control (CMC) problems related to light chain mispairing or heavy chain mispairing. [Prior art documents] [Non-patent literature]

[0011] [Non-Patent Document 1] Brennan, M et al., Science, 1985, Vol. 229 (No. 4708): pp. 81-83. [Non-patent document 2] Glennie, MJ et al., J Immunol, 1987, 139(7):2367-2375 Summary of the Invention [Problem to be solved by the invention]

[0012] 3. Overview of this technology In some embodiments, the technology relates to polypeptides (or ISVD constructs) that specifically target TNFα and OX40L simultaneously, leading to increased efficiency in modulating the inflammatory response compared to monospecific anti-TNFα or anti-OX40L polypeptides. [Means for solving the problem]

[0013] In some embodiments, the polypeptides of the present technology are efficiently produced (e.g., in microbial hosts) and have low viscosity at high concentrations, which is advantageous and convenient for subcutaneous administration. Furthermore, in some embodiments, the polypeptides of the present technology have limited reactivity with the pre-existing antibodies of the subject to be treated (i.e., antibodies present in the subject before the first treatment with the antibody construct). In preferred embodiments, such polypeptides exhibit a sufficiently long half-life in the subject to be treated so that successive treatments can be conveniently spaced apart.

[0014] The polypeptide of the present technology comprises or consists of at least four immunoglobulin single variable domains (ISVDs), wherein at least two ISVDs specifically bind to TNFα and at least two ISVDs specifically bind to OX40L. Preferably, the at least two ISVDs that bind to TNFα specifically bind to human TNFα, and the at least two ISVDs that bind to OX40L specifically bind to human OX40L.

[0015] The polypeptide preferably further comprises one or more other groups, residues, moieties, or binding units, optionally linked via one or more peptide linkers, which provide the polypeptide with an increased half-life compared to a corresponding polypeptide that does not have the one or more other groups, residues, moieties, or binding units. For example, the binding unit may be an ISVD that binds to a serum protein, preferably a human serum protein such as human serum albumin.

[0016] Also provided are nucleic acid molecules capable of expressing the polypeptides of the present technology, vectors comprising the nucleic acids or nucleic acids, and compositions comprising the polypeptides, nucleic acids, or vectors. The compositions are preferably pharmaceutical compositions.

[0017] Also provided are hosts or host cells comprising a nucleic acid or vector encoding a polypeptide according to the technology.

[0018] 1. A method for producing a polypeptide according to the present technology, comprising: a. optionally expressing a nucleic acid sequence encoding a polypeptide according to the present technology in a suitable host cell or host organism or in another suitable expression system, optionally followed by: b. Isolating and / or purifying the polypeptide according to the present technology There is further provided a method comprising at least

[0019] Furthermore, the present technology provides a polypeptide, a composition comprising the polypeptide, or a composition comprising a nucleic acid or vector comprising a nucleotide sequence encoding the polypeptide, for use as a medicament. Preferably, the polypeptide or composition is for use in treating an autoimmune or inflammatory disease, and preferably, the autoimmune or inflammatory disease is selected from rheumatoid arthritis, inflammatory bowel disease such as Crohn's disease and ulcerative colitis, psoriasis, hidradenitis suppurativa, and graft-versus-host disease.

[0020] In addition, a method for treating autoimmune or inflammatory disease is provided, comprising administering a pharmaceutically active amount of a polypeptide or composition according to the present technology to a subject in need thereof.The autoimmune or inflammatory disease is preferably selected from rheumatoid arthritis, inflammatory bowel diseases such as Crohn's disease and ulcerative colitis, and hidradenitis suppurativa.In a preferred embodiment, this method further comprises administering one or more additional therapeutic agents, such as methotrexate.

[0021] Further provided is the use of a polypeptide or composition of the present technology in the preparation of a pharmaceutical composition for treating an autoimmune or inflammatory disease, wherein the autoimmune or inflammatory disease is preferably selected from rheumatoid arthritis, inflammatory bowel disease such as Crohn's disease and ulcerative colitis, psoriasis, hidradenitis suppurativa, and graft-versus-host disease.

[0022] In particular, the present technology provides the following embodiments: Embodiment 1: A polypeptide, a composition comprising a polypeptide, or a composition comprising a nucleic acid comprising a nucleotide sequence encoding a polypeptide, for use as a medicament, wherein the polypeptide comprises or consists of at least four immunoglobulin single variable domains (ISVDs), each of the ISVDs comprising three complementarity determining regions (CDR1-CDR3, respectively), optionally linked via one or more peptide linkers; a. The first ISVD and the second ISVD are: i. CDR1 comprising the amino acid sequence of SEQ ID NO: 7 or having two or one amino acid difference from SEQ ID NO: 7; ii. CDR2 comprising the amino acid sequence of SEQ ID NO: 10 or having two or one amino acid difference from SEQ ID NO: 10; and iii. A CDR3 comprising the amino acid sequence of SEQ ID NO: 13 or having two or one amino acid difference from SEQ ID NO: 13. Includes; b. The third and fourth ISVDs are: iv. CDR1 comprising the amino acid sequence of SEQ ID NO: 8 or having two or one amino acid difference from SEQ ID NO: 8; v. CDR2 comprising the amino acid sequence of SEQ ID NO: 11 or having two or one amino acid difference from SEQ ID NO: 11; and vi. CDR3 comprising the amino acid sequence of SEQ ID NO: 14 or having two or one amino acid difference from SEQ ID NO: 14 Including, ISVD is a polypeptide, a composition comprising a polypeptide, or a composition comprising a nucleic acid comprising a nucleotide sequence encoding a polypeptide, in order starting from the N-terminus.

[0023] Embodiment 2: A composition for use according to embodiment 1, which is a pharmaceutical composition further comprising at least one pharmaceutically acceptable carrier, diluent or excipient, and / or adjuvant, and optionally one or more additional pharmaceutically active polypeptides and / or compounds.

[0024] Embodiment 3: a. the first ISVD and the second ISVD comprise a CDR1 comprising the amino acid sequence of SEQ ID NO:7, a CDR2 comprising the amino acid sequence of SEQ ID NO:10, and a CDR3 comprising the amino acid sequence of SEQ ID NO:13; b. A polypeptide or composition for use according to embodiment 1 or 2, wherein the third ISVD and the fourth ISVD comprise a CDR1 comprising the amino acid sequence of SEQ ID NO: 8, a CDR2 comprising the amino acid sequence of SEQ ID NO: 11, and a CDR3 comprising the amino acid sequence of SEQ ID NO: 14.

[0025] Embodiment 4: a. the amino acid sequence of the first ISVD comprises greater than 90% sequence identity with SEQ ID NO:2; b. the amino acid sequence of the second ISVD comprises greater than 90% sequence identity to SEQ ID NO:3; c. the amino acid sequence of the third ISVD comprises greater than 90% sequence identity to SEQ ID NO:4; d. A polypeptide or composition for use according to any one of embodiments 1 to 3, wherein the amino acid sequence of the fourth ISVD comprises greater than 90% sequence identity with SEQ ID NO:6.

[0026] Embodiment 5: a. the first ISVD comprises the amino acid sequence of SEQ ID NO:2; b. the second ISVD comprises the amino acid sequence of SEQ ID NO:3; c. the third ISVD comprises the amino acid sequence of SEQ ID NO:4; d. The polypeptide or composition for use of any one of embodiments 1 to 4, wherein the fourth ISVD comprises the amino acid sequence of SEQ ID NO: 6.

[0027] Embodiment 6: Polypeptide or composition for use according to any one of embodiments 1 to 5, wherein the polypeptide further comprises one or more other groups, residues, moieties or binding units, optionally linked via one or more peptide linkers, and wherein the one or more other groups, residues, moieties or binding units provide the polypeptide with an increased half-life compared to a corresponding polypeptide that does not have the one or more other groups, residues, moieties or binding units.

[0028] Embodiment 7: The polypeptide or composition for use according to embodiment 6, wherein the one or more other groups, residues, moieties, or binding units that provide the polypeptide with increased half-life are selected from the group consisting of polyethylene glycol molecules, serum proteins or fragments thereof, binding units capable of binding to serum proteins, Fc moieties, and small proteins or peptides capable of binding to serum proteins.

[0029] Embodiment 8: Polypeptide or composition for use according to embodiment 6 or 7, wherein the one or more other groups, residues, moieties or binding units that provide the polypeptide with increased half-life are selected from the group consisting of binding units capable of binding to serum albumin (such as human serum albumin) or serum immunoglobulin (such as IgG).

[0030] Embodiment 9: Polypeptide or composition for use according to embodiment 8, wherein the binding unit that confers an increased half-life to the polypeptide is an ISVD capable of binding to human serum albumin.

[0031] Embodiment 10: An ISVD that binds to human serum albumin is i. CDR1 comprising the amino acid sequence of SEQ ID NO: 9 or having two or one amino acid difference from SEQ ID NO: 9; ii. CDR2 comprising the amino acid sequence of SEQ ID NO: 12 or having two or one amino acid difference from SEQ ID NO: 12; and iii. CDR3 comprising the amino acid sequence of SEQ ID NO: 15 or having two or one amino acid difference from SEQ ID NO: 15 10. The polypeptide or composition for use according to embodiment 9, comprising:

[0032] Embodiment 11: A polypeptide or composition for use according to embodiment 9 or 10, wherein the ISVD that binds to human serum albumin comprises a CDR1 comprising the amino acid sequence of SEQ ID NO: 9, a CDR2 comprising the amino acid sequence of SEQ ID NO: 12, and a CDR3 comprising the amino acid sequence of SEQ ID NO: 15.

[0033] Embodiment 12: A polypeptide or composition for use according to any one of embodiments 9 to 11, wherein the amino acid sequence of the ISVD that binds to human serum albumin comprises more than 90% sequence identity with SEQ ID NO: 5.

[0034] Embodiment 13: Polypeptide or composition for use according to any one of embodiments 9 to 12, wherein the ISVD that binds to human serum albumin comprises the amino acid sequence of SEQ ID NO: 5.

[0035] Embodiment 14: A polypeptide or composition for use according to any one of embodiments 1 to 13, wherein the amino acid sequence of the polypeptide comprises more than 90% sequence identity with SEQ ID NO: 1.

[0036] Embodiment 15: Polypeptide or composition for use according to any one of embodiments 1 to 14, wherein the polypeptide comprises or consists of the amino acid sequence of SEQ ID NO: 1.

[0037] Embodiment 16: A polypeptide or composition for use according to any one of embodiments 1 to 15 for use in the treatment of an autoimmune or inflammatory disease.

[0038] Embodiment 17: The polypeptide or composition for use according to embodiment 16, wherein the autoimmune or inflammatory disease is selected from rheumatoid arthritis, inflammatory bowel diseases such as Crohn's disease and ulcerative colitis, psoriasis, hidradenitis suppurativa, and graft-versus-host disease.

[0039] Embodiment 18: A polypeptide comprising a nucleic acid comprising a nucleotide sequence encoding a polypeptide, wherein the polypeptide comprises or consists of at least four immunoglobulin single variable domains (ISVDs), each of the ISVDs comprising three complementarity determining regions (CDR1-CDR3, respectively), optionally linked via one or more peptide linkers; a. The first ISVD and the second ISVD are: i. CDR1 comprising the amino acid sequence of SEQ ID NO: 7 or having two or one amino acid difference from SEQ ID NO: 7; ii. CDR2 comprising the amino acid sequence of SEQ ID NO: 10 or having two or one amino acid difference from SEQ ID NO: 10; and iii. A CDR3 comprising the amino acid sequence of SEQ ID NO: 13 or having two or one amino acid difference from SEQ ID NO: 13. Includes; b. The third and fourth ISVDs are: iv. CDR1 comprising the amino acid sequence of SEQ ID NO: 8 or having two or one amino acid difference from SEQ ID NO: 8; v. CDR2 comprising the amino acid sequence of SEQ ID NO: 11 or having two or one amino acid difference from SEQ ID NO: 11; and vi. CDR3 comprising the amino acid sequence of SEQ ID NO: 14 or having two or one amino acid difference from SEQ ID NO: 14 Including, ISVD is the order of the polypeptides starting from the N-terminus.

[0040] Embodiment 19: a. the first ISVD and the second ISVD comprise a CDR1 comprising the amino acid sequence of SEQ ID NO:7, a CDR2 comprising the amino acid sequence of SEQ ID NO:10, and a CDR3 comprising the amino acid sequence of SEQ ID NO:13; b. The polypeptide of embodiment 18, wherein the third ISVD and the fourth ISVD comprise a CDR1 comprising the amino acid sequence of SEQ ID NO: 8, a CDR2 comprising the amino acid sequence of SEQ ID NO: 11, and a CDR3 comprising the amino acid sequence of SEQ ID NO: 14.

[0041] Embodiment 20: a. the amino acid sequence of the first ISVD comprises greater than 90% sequence identity with SEQ ID NO:2; b. the amino acid sequence of the second ISVD comprises greater than 90% sequence identity to SEQ ID NO:3; c. the amino acid sequence of the third ISVD comprises greater than 90% sequence identity to SEQ ID NO:4; d. The polypeptide of embodiment 18 or 19, wherein the amino acid sequence of the fourth ISVD comprises greater than 90% sequence identity with SEQ ID NO:6.

[0042] Embodiment 21: a. the first ISVD comprises the amino acid sequence of SEQ ID NO:2; b. the second ISVD comprises the amino acid sequence of SEQ ID NO:3; c. the third ISVD comprises the amino acid sequence of SEQ ID NO:4; d. The polypeptide of any one of embodiments 18 to 20, wherein the fourth ISVD comprises the amino acid sequence of SEQ ID NO:6.

[0043] Embodiment 22: The polypeptide of any one of embodiments 18 to 21, wherein the polypeptide further comprises one or more other groups, residues, moieties or binding units, optionally linked via one or more peptide linkers, and wherein the one or more other groups, residues, moieties or binding units provide the polypeptide with an increased half-life compared to a corresponding polypeptide that does not have the one or more other groups, residues, moieties or binding units.

[0044] Embodiment 23: The polypeptide of embodiment 22, wherein the one or more other groups, residues, moieties, or binding units that provide the polypeptide with increased half-life are selected from the group consisting of polyethylene glycol molecules, serum proteins or fragments thereof, binding units capable of binding to serum proteins, Fc portions, and small proteins or peptides capable of binding to serum proteins.

[0045] Embodiment 24: The polypeptide of embodiment 22 or 23, wherein the one or more other groups, residues, moieties, or binding units that provide the polypeptide with increased half-life are selected from the group consisting of binding units capable of binding to serum albumin (such as human serum albumin) or serum immunoglobulin (such as IgG).

[0046] Embodiment 25: The polypeptide of embodiment 24, wherein the binding unit that confers an increased half-life to the polypeptide is an ISVD capable of binding to human serum albumin.

[0047] Embodiment 26: The ISVD that binds to human serum albumin is i. CDR1 comprising the amino acid sequence of SEQ ID NO: 9 or having two or one amino acid difference from SEQ ID NO: 9; ii. CDR2 comprising the amino acid sequence of SEQ ID NO: 12 or having two or one amino acid difference from SEQ ID NO: 12; and iii. CDR3 comprising the amino acid sequence of SEQ ID NO: 15 or having two or one amino acid difference from SEQ ID NO: 15 26. The polypeptide of embodiment 25, comprising:

[0048] Embodiment 27: A polypeptide or composition for use according to embodiment 25 or 26, wherein the ISVD that binds to human serum albumin comprises a CDR1 comprising the amino acid sequence of SEQ ID NO: 9, a CDR2 comprising the amino acid sequence of SEQ ID NO: 12, and a CDR3 comprising the amino acid sequence of SEQ ID NO: 15.

[0049] Embodiment 28: A polypeptide or composition for use according to any one of embodiments 25 to 27, wherein the amino acid sequence of the ISVD that binds to human serum albumin comprises more than 90% sequence identity with SEQ ID NO: 5.

[0050] Embodiment 29: The polypeptide of any one of embodiments 25 to 28, wherein the ISVD that binds to human serum albumin comprises the amino acid sequence of SEQ ID NO: 5.

[0051] Embodiment 30: The polypeptide of any one of embodiments 18 to 29, wherein the amino acid sequence of the polypeptide comprises more than 90% sequence identity with SEQ ID NO:1.

[0052] Embodiment 31: Polypeptide or composition for use according to any one of embodiments 18 to 30, wherein the polypeptide comprises or consists of the amino acid sequence of SEQ ID NO: 1.

[0053] Embodiment 32: A nucleic acid comprising a nucleotide sequence encoding a polypeptide according to any one of embodiments 18 to 31.

[0054] Embodiment 33: A host or host cell comprising a nucleic acid according to embodiment 32.

[0055] Embodiment 34: A method for producing a polypeptide according to any one of embodiments 18 to 31, comprising: a. expressing the nucleic acid of embodiment 32 in a suitable host cell or host organism or in another suitable expression system; optionally followed by: b. Isolating and / or purifying the polypeptide according to any one of embodiments 18 to 31. The method includes at least the following.

[0056] Embodiment 35: A composition comprising at least one polypeptide according to any one of embodiments 18 to 31 or a nucleic acid according to embodiment 32.

[0057] Embodiment 36: The composition of embodiment 35, which is a pharmaceutical composition, further comprising at least one pharmaceutically acceptable carrier, diluent or excipient, and / or adjuvant, and optionally one or more additional pharmaceutically active polypeptides and / or compounds.

[0058] Embodiment 37: A method of treating an autoimmune or inflammatory disease, comprising administering to a subject in need thereof a pharmaceutically active amount of the polypeptide of any one of embodiments 18 to 31 or the composition of embodiment 35 or 36.

[0059] Embodiment 38: The method of embodiment 37, wherein the autoimmune or inflammatory disease is selected from rheumatoid arthritis, inflammatory bowel disease such as Crohn's disease and ulcerative colitis, psoriasis, hidradenitis suppurativa, and graft-versus-host disease.

[0060] Embodiment 39: The method of embodiment 37 or 38, further comprising administering one or more additional therapeutic agents.

[0061] Embodiment 40: The method of embodiment 39, wherein the additional therapeutic agent is methotrexate.

[0062] Embodiment 41: Use of a polypeptide according to any one of embodiments 18 to 31 or a composition according to embodiment 35 or 36 in the preparation of a pharmaceutical composition for treating an autoimmune or inflammatory disease.

[0063] Embodiment 42: Use of a polypeptide or composition according to embodiment 41, wherein the autoimmune or inflammatory disease is selected from rheumatoid arthritis, inflammatory bowel diseases such as Crohn's disease and ulcerative colitis, psoriasis, hidradenitis suppurativa, graft-versus-host disease. [Brief explanation of the drawings]

[0064] [Figure 1]FIG. 10 shows a sensorgram showing simultaneous binding of recombinant soluble hTNFα and hOX40L to ISVD construct F027300252 captured by HSA. [Figure 2] Figure 1 shows simultaneous binding of soluble TNFα and membrane-bound hOX40L to ISVD construct F027300252 as demonstrated by flow cytometry on CHO-Ki cells expressing human OX40L. IRR00096 is a negative control VHH. [Figure 3] Figure 1 shows the inhibition of soluble human and cynomolgus TNFα by ISVD construct F0275000252 and reference compound anti-hTNFα Reference mAb in a Glo response™ HEK293_NFκB-NLucP reporter assay. IRR00096 is a negative control VHH. [Figure 4] FIG. 1 shows the inhibition of membrane-bound OX40L by ISVD construct F0275000252 and the reference compound anti-hOX40L mAb as measured in a PBMC activity assay. [Figure 5] Figure 1 shows the induction of luciferase activity [RLU] by 5 ng / ml recombinant human TNFα or 100 ng / ml human OX40L or a combination of both, and the inhibition of induced luciferase activity by various concentrations from 0.5 μg / ml to 5 μg / ml of an anti-TNFα antibody (PB03017; from Sanofi), an anti-OX40L antibody (catalog no. AB00536, from Absolute Antibody), or a combination of both antibodies. [Figure 6]Figure 5 shows the induction of luciferase activity [RLU] by a combination of recombinant human TNFα and human OX40L (as described in Figure 5), and the inhibition of induced luciferase activity by the monospecific anti-OX40L VHH ALX-0632 or the monospecific anti-TNF VHH ATN-103, or the anti-TNF / anti-OX40L bispecific ISVD constructs F027300252, F027301104, F027301189, F027301197, and F027301199. The % maximum inhibition of NFκB luciferase activity achieved by the bispecific or monospecific ISVD constructs / VHHs is shown. [Figure 7] Figure 1 shows the induction of luciferase activity by a combination of recombinant human TNFα and human OX40L (as described in Figure 5), and the inhibition of induced luciferase activity by anti-TNF / anti-OX40L bispecific ISVD constructs F027300252, F027301104, F027301189, F027301197, and F027301199. Mean IC50 values ​​(pM) ± SD from at least three independent experiments. [Figure 8]

[0023] Figure 1 shows surface expression of OX40L on human monocyte-derived dendritic cells at days 1, 2, and 3 of maturation. OX40L expression was measured by flow cytometry. Results represent the mean ± SEM of three different human mDC donors tested against PBMCs from five allogeneic donors. [Figure 9] Figure 1 shows GM-CSF expression in MLR assays on day 5. GM-CSF secretion was measured in supernatants from MLR assays after incubation with anti-TNFα antibody alone [10 μg / ml], anti-OX40L antibody alone [10 μg / ml], or the combination of anti-TNF [10 μg / ml] and anti-OX40L [10 μg / ml] antibodies. Results from five PBMC donors tested against DCs derived from three allogeneic DC donors. **p<0.0016, ****p<0.0001. [Figure 10]FIG. 1 shows box plots showing binding of pre-existing antibodies present in 96 human serum samples to ISVD constructs F027300252, F027301104, F027301189, and F027301197 compared to control ISVD constructs F027301099 and F027301186. [Figure 11] FIG. 1 shows box plots showing binding of pre-existing antibodies present in 96 human serum samples to ISVD constructs F027300028, F027300252, F027301097, and F027301186. [Figure 12] Figure 1 shows arthritis scores over time in different treatment groups (n=8 mice / group). Animals received intraperitoneal injections of the title compound twice a week starting at 6 weeks of age. Shown are the mean weekly arthritis scores ± SEM. Statistics: Two-way ANOVA and Bonferroni multiple comparison test. ns (not significant) p>0.05, *p<0.05, **p<0.01. ***p<0.001, ****p<0.0001. [Figure 13] Figure 1 shows the area under the curve for arthritis scores over time. Individual values ​​(symbols) and mean ± SEM (bars) are shown. Statistics are one-way ANOVA and Bonferroni multiple comparison test. ns (not significant) p>0.05, *p<0.05, **p<0.01. ***p<0.001, ****p<0.0001. [Figure 14] Figure 1 shows histology scores. Individual values ​​(symbols) and mean ± SEM (bars) are shown. Statistics are one-way ANOVA and Bonferroni multiple comparison test. [Figure 15]

[0023] Figure 1 shows the study design for testing the efficacy of the anti-TNFα-OX40L ISVD construct F027300252 (referred to as nAb) in the TNFα-humanized mouse collagen antibody-induced arthritis (CAIA) model. The anti-hTNFα reference mAb was dosed simultaneously with the ISVD construct, with the first dose 6 hours after LPS and the second dose on day 4, 3 days after LPS. [Figure 16]Figure 10 shows the arthritis score evolution over time in an experiment in a mouse model of CAIA (n=8 mice / group excluding vehicle; n=16 / group from two independent experiments). Animals received two intraperitoneal injections of either vehicle, the reference compound anti-hTNFα reference mAb, or the half-life extended anti-TNFα-OX40L ISVD construct F027300252 according to the scheme in Figure 10. Statistics are two-way ANOVA and Bonferroni multiple comparison test. [Figure 17] FIG. 1 shows the AUC of experimental arthritis scores in a mouse model of CAIA (n=8 mice / group excluding vehicle: n=16 / group from two independent experiments). [Figure 18] Figure 1 shows the study scheme for the TDAR-DTH combination model. The light gray box above the daily time course indicates KLH administration during the TDAR portion of the study. The dark gray box indicates the intramuscular injection of the second antigen, tetanoid toxin (TTx), with aluminum hydroxide (ALU) during the DTH portion of the study. The white boxes above the time course indicate skin challenges with TTx / ALU and KLH on days 31 and 56, with the DTH model indicated by the dark arrows. Skin biopsies evaluated for histopathology and immunohistochemistry are shown on day 34, and necropsies on day 59. After TTx / ALU and KLH challenge, skin areas were followed for 24 / 48 / 72 hours for each DTH challenge, and in-life changes were assessed as described in Table 15. [Figure 19] FIG. 1 shows the mean serum concentrations (ng / mL) of F027300252 following subcutaneous administration to female monkeys at 3, 10, 30, and 100 mg / kg / adm on days 1 and 29 (semi-logarithmic scale plot). [Figure 20]This figure shows the anti-KLH response of cynomolgus monkey TDAR, focusing on the anti-KLH IgG response. Four cynomolgus monkeys were used in each group. Data are shown as mean ± SD. D3 (day 3) and d31 (day 31) mark the time points of KLH stimulation. The primary response spans the time frame from BL (-12D) to day 30 (30D), and the secondary response spans the time frame from day 31 (31D) to the end of the study, day 59 (59D). F027300252 was administered in a dose-dependent manner by weekly subcutaneous injection from 3 mg / kg to 100 mg / kg. Treatment was discontinued after the fifth injection on day 29. Statistics were two-way ANOVA and Bonferroni's multiple comparison test applied to the secondary response. There were no significant differences from vehicle in all treatment groups during the primary response. [Figure 21] 1 shows the mean percentage reduction in anti-KLH IgG AUC during the TDAR secondary response from 4 mg / kg anti-hTNFα reference mAb and 8 mg / kg anti-hOX40L reference mAb vehicle (pilot study), and from different dose levels of F027300252 (3, 10, 30, and 100 mg / kg) in the TDAR monkey study. Doses are expressed in nmol / kg. [Figure 22] Figure 1 shows IFN-γ spot-forming cells / million cells after KLH restimulation of PBMCs on day 59, as determined by ELISPOT assay. Bars represent mean ± SD. Individual animals are depicted as either filled circles (vehicle control) or open circles (F027300252-treated groups). For F027300252, the respective doses are given in mg / kg. In the group in which one animal was administered 30 mg / kg, the assay did not meet quality control standards, so only three of four animals are shown. Statistics are one-way ANOVA and Bonferroni's multiple comparison test. [Figure 23]Figure 1 shows IL-4 spot-forming cells / million cells after KLH restimulation of PBMCs on day 59, as determined by ELISPOT assay. Bars represent mean ± SD. Individual animals are depicted as either filled circles (vehicle control) or open circles (F027300252-treated groups). For F027300252, the respective doses are given in mg / kg. In the group in which one animal was administered 30 mg / kg, the assay did not meet quality control standards, so only three of four animals are shown. Statistics are one-way ANOVA and Bonferroni's multiple comparison test. [Figure 24] Schematic representation of various immunizations for either TDAR (light gray) and DTH (dark gray) on the left side of the cartoon. For DTH, animals were challenged with TTX / ALU and KLH on either day 31 or day 59, respectively. Different intradermal injection sites were used, as illustrated in the central cartoon. Biopsies 8 mm in diameter were taken at the end of the study on days 34 and 59 and evaluated by histopathology and immunohistochemistry (right part of the cartoon). [Figure 25] Figure 1 shows the evolution of GVHD scores over time in a xenogeneic GVHD mouse model. Data are shown as mean ± SEM. N=7, 2 different hPBMC donors. Statistics: One-way ANOVA and Bonferroni multiple comparison test. [Figure 26] Figure 1 shows survival over time in a xenogeneic GVHD mouse model. Data are presented as Kaplan-Meier survival curves. n=7, two different hPBMC donors. Survival data were analyzed using the log-rank (Mantel-Cox) test. P values ​​were corrected for multiple comparisons. [Figure 27] Figure 1 shows hPBMC engraftment in recipient NSG mice. Data are shown as mean ± SEM. n=7, 2 different hPBMC donors. Kaplan engraftment data were analyzed using mixed-effects analysis and Bonferroni multiple comparison test. [Figure 28]Figure 1 shows GVHD score evolution over time in a xenogeneic GVHD mouse model. Data are shown as mean ± SEM. Results are pooled from two independent studies. n = 7-12, three different hPBMC donors. Statistics: One-way ANOVA and Bonferroni multiple comparison test. [Figure 29] Figure 1 shows survival over time in a xenogeneic GVHD mouse model. Data are presented as Kaplan-Meier survival curves. Results are pooled from two independent studies. n = 7-12, three different hPBMC donors. Survival data were analyzed using the log-rank (Mantel-Cox) test. P values ​​were corrected for multiple comparisons. [Figure 30] Figure 1. Engraftment of hPBMCs in host NSG mice. Data are shown as mean ± SEM. Results are pooled from two independent studies; n = 7-12, three different hPBMC donors. Engraftment data were analyzed using mixed-effects analysis and Bonferroni multiple comparison tests. [Figure 31] Figure 1 shows inhibition of PHA-induced IL-8 release in human whole blood by the monospecific anti-TNF monoclonal antibody RA14956298 and the bispecific anti-TNFα / anti-OX40L ISVD constructs F027300252, F027301104, F027301189, F027301197, and F027301199. Values ​​correspond to the mean IC50 [nM] ± SEM and represent the results of three different donors, each in triplicate. [Figure 32] FIG. 1 is a schematic diagram of ISVD construct F027300252 showing monovalent building blocks / ISVDs 1E07 / 1, 1C02 / 1, and ALB23002 connected via a 9GS linker from N- to C-terminus. DETAILED DESCRIPTION OF THE INVENTION

[0065] 5. Detailed Description of This Technology The aim of this technology is to provide a new type of drug for treating autoimmune or inflammatory diseases.

[0066] The present inventors have surprisingly found that polypeptides comprising at least four ISVDs, wherein at least two ISVDs specifically bind to TNFα, preferably human TNFα, and at least two ISVDs specifically bind to OX40L, preferably human OX40L, can be used for more efficient treatment of autoimmune or inflammatory diseases compared to monospecific anti-TNFα or anti-OX40L polypeptides. In some embodiments, the polypeptides of the present technology are efficiently produced (e.g., in microbial hosts) and exhibit low viscosity at high concentrations, which is advantageous and convenient for subcutaneous administration. Furthermore, such polypeptides have limited reactivity with pre-existing antibodies of the subject to be treated (i.e., antibodies present in the subject before the first treatment with the antibody construct). In a preferred embodiment, such polypeptides exhibit a sufficiently long half-life in the subject to be treated so that successive treatments can be conveniently spaced apart.

[0067] The polypeptides are at least bispecific, but may be, for example, trispecific, tetraspecific, or pentaspecific. Additionally, the polypeptides are at least tetravalent, but may be, for example, pentavalent or hexavalent.

[0068] The terms "bispecific," "trispecific," "tetraspecific," or "pentaspecific" all fall within the term "multispecific" and refer to binding to two, three, four, or five different target molecules, respectively. The terms "bivalent," "trivalent," "tetravalent," "pentavalent," or "hexavalent" all fall within the term "multivalent" and refer to the presence of two, three, four, or five binding units (e.g., ISVDs), respectively. For example, a polypeptide may be trispecific pentavalent, such as a polypeptide comprising or consisting of five ISVDs (e.g., ISVD construct F027300252), in which two ISVDs bind to human TNFα, two ISVDs bind to human OX40L, and one ISVD binds to human serum albumin. Such a polypeptide may simultaneously be dual paratopic, for example, if the two ISVDs bind to two different epitopes on human TNFα or human OX40L. The term "dual paratopic" refers to binding to two different portions (eg, epitopes) of the same target molecule.

[0069] As used herein, terms such as "first ISVD," "second ISVD," and "third ISVD" merely indicate the relative positions of the ISVDs relative to one another, and numbering begins from the N-terminus of the polypeptide of the present technology. Thus, the "first ISVD" is closer to the N-terminus than the "second ISVD," which is closer to the N-terminus than the "third ISVD," and so on. Thus, the ISVD arrangement is reversed when considered from the C-terminus. Because the numbering is not absolute and merely indicates the relative positions of at least three ISVDs, it does not exclude the possibility that other binding units / building blocks, such as additional ISVDs that bind to TNFα or OX40L or ISVDs that bind to other targets, may be present in the polypeptide. Furthermore, it does not exclude the possibility that other binding units / building blocks, such as ISVDs, may be interposed. For example, as described further below (see in particular Section 5.3 "(In Vivo) Half-Life Extension"), the polypeptide may further comprise another ISVD that binds to human serum albumin, which may even be located, for example, between the "second ISVD" and the "third ISVD".

[0070] In light of the above, the present technology provides a polypeptide comprising or consisting of at least four ISVDs, wherein at least two ISVDs specifically bind to TNFα and at least two ISVDs specifically bind to OX40L, and wherein the TNFα and OX40L are preferably human TNFα and human OX40L.

[0071] The components of the polypeptide, preferably the ISVDs, may be linked to each other by one or more suitable linkers, such as peptide linkers.

[0072] The use of linkers to connect two or more (poly)peptides is well known in the art. Exemplary peptide linkers are shown in Table A-5. One commonly used class of peptide linkers is known as "Gly-Ser" or "GS" linkers. These are linkers consisting essentially of glycine (G) and serine (S) residues, and typically contain one or more repeats of a peptide motif, such as the GGGGS (SEQ ID NO: 60) motif (e.g., a sequence of the formula (Gly-Gly-Gly-Gly-Ser) n (wherein n is 1, 2, 3, 4, 5, 6, 7, or more). Some commonly used examples of such GS linkers are the 9GS linker (GGGGSGGGS, SEQ ID NO: 63), the 15GS linker (n=3), and the 35GS linker (n=7). See, for example, Chen et al., Adv. Drug Deliv. Rev. 2013, October 15; 65(10):1357-1369; and Klein et al., Protein Eng. Des. Sel. (2014) 27(10):325-330. In the polypeptide of the present technology, it is preferred to use a 9GS linker to link the components of the polypeptide to each other.

[0073] In a preferred embodiment, two of the at least two ISVDs that specifically bind to TNFα are located at the C-terminus of the polypeptide. The present inventors have surprisingly found that such a configuration can increase the production yield of the polypeptide.

[0074] Also, in a preferred embodiment, two of the at least two ISVDs that specifically bind to OX40L are positioned at the N-terminus of the polypeptide.

[0075] Thus, the polypeptide preferably comprises or consists, in order starting from the N-terminus of the polypeptide, of a first ISVD that specifically binds OX40L, a second ISVD that specifically binds OX40L, a first ISVD that specifically binds TNFα, an optional binding unit that confers increased half-life to the polypeptide, as defined herein, and a second ISVD that specifically binds TNFα. The binding unit that confers increased half-life to the polypeptide is preferably an ISVD.

[0076] It is further preferred that the polypeptide comprises or consists of, in order starting from the N-terminus of the polypeptide: an ISVD that specifically binds to OX40L, a linker, a second ISVD that specifically binds to OX40L, a linker, a first ISVD that specifically binds to TNFα, a linker, an ISVD that specifically binds to human serum albumin, a linker, and a second ISVD that specifically binds to TNFα, each linker preferably being a 9GS linker.

[0077] Such configuration of the polypeptide can provide increased production yield, good CMC characteristics, as well as optimized functionality and greater potency with respect to modulating the immune response.

[0078] Preferably, the polypeptides of the present technology exhibit reduced binding by pre-existing antibodies in human serum. To this end, in one embodiment, the polypeptide comprises a valine (V) at amino acid position 11 and a leucine (L) at amino acid position 89 (according to Kabat numbering) in at least one ISVD, but preferably in each ISVD. In another embodiment, the polypeptide comprises an extension of one to five (preferably naturally occurring) amino acids, such as a single alanine (A) extension, at the C-terminus of the C-terminal ISVD. The C-terminus of the ISVD is typically VTVSS (SEQ ID NO: 125). In another embodiment, the polypeptide comprises a lysine (K) or glutamine (Q) at position 110 (according to Kabat numbering) in at least one ISVD. In another embodiment, the ISVD comprises a lysine (K) or glutamine (Q) at position 112 (according to Kabat numbering) in at least one ISVD. In such embodiments, the C-terminus of the ISVD is VKVSS (SEQ ID NO: 126), VQVSS (SEQ ID NO: 127), VTVKS (SEQ ID NO: 131), VTVQS (SEQ ID NO: 132), VKVKS (SEQ ID NO: 133), VKVQS (SEQ ID NO: 134), VQVKS (SEQ ID NO: 135), or VQVQS (SEQ ID NO: 136), and thus the C-terminus of the polypeptide after the addition of a single alanine comprises, for example, the sequence VTVSSA (SEQ ID NO: 128), VKVSSA (SEQ ID NO: 129), VQVSSA (SEQ ID NO: 130), VTVKSA (SEQ ID NO: 137), VTVQSA (SEQ ID NO: 138), VKVKSA (SEQ ID NO: 139), VKVQSA (SEQ ID NO: 140), VQVKSA (SEQ ID NO: 141), or VQVQSA (SEQ ID NO: 142), preferably VKVSSA (SEQ ID NO: 129). In another embodiment, the polypeptide comprises a valine (V) at amino acid position 11 and a leucine (L) at amino acid position 89 (according to Kabat numbering) of each ISVD, optionally a lysine (K) or glutamine (Q) at position 110 (according to Kabat numbering) of at least one ISVD, and comprises an extension of 1 to 5 (preferably naturally occurring) amino acids at the C-terminus of the C-terminal ISVD, such as a single alanine (A) extension (thus the C-terminus of the polypeptide comprises, for example, the sequence VTVSSA (SEQ ID NO: 128), VKVSSA (SEQ ID NO: 129), or VQVSSA (SEQ ID NO: 130), preferably VKVSSA (SEQ ID NO: 129)). For further information in this regard, see, e.g., WO 2012 / 175741 and WO 2015 / 173325.

[0079] In a preferred embodiment, the polypeptide of the present technology comprises or consists of an amino acid sequence comprising greater than 90% sequence identity, such as greater than 95% or greater than 99%, to SEQ ID NO: 1, and optionally the CDRs of the five ISVDs are as defined in items A-C (or A'-C', if the Kabat definition is used) set forth in sections "5.1 Immunoglobulin Single Variable Domains" and "5.3 (In Vivo) Half-Life Extension" below, respectively, and in particular: The first and second ISVDs that specifically bind to OX40L are amino acids of SEQ ID NO: 7. CDR1 comprising the amino acid sequence of SEQ ID NO: 10, CDR2 comprising the amino acid sequence of SEQ ID NO: 10, and CDR3 comprising the amino acid sequence of SEQ ID NO: 13; the third and fourth ISVDs that specifically bind to TNFα have a CDR1 comprising the amino acid sequence of SEQ ID NO:8, a CDR2 comprising the amino acid sequence of SEQ ID NO:11, and a CDR3 comprising the amino acid sequence of SEQ ID NO:14; the ISVD that binds to human serum albumin comprises a CDR1 comprising the amino acid sequence of SEQ ID NO:9, a CDR2 comprising the amino acid sequence of SEQ ID NO:12, and a CDR3 comprising the amino acid sequence of SEQ ID NO:15; or Or alternatively, if you use the Kabat Provisions: the first and second ISVDs that specifically bind to OX40L have a CDR1 comprising the amino acid sequence of SEQ ID NO: 28, a CDR2 comprising the amino acid sequence of SEQ ID NO: 31, and a CDR3 comprising the amino acid sequence of SEQ ID NO: 13; the third and fourth ISVDs that specifically bind to TNFα have a CDR1 comprising the amino acid sequence of SEQ ID NO: 29, a CDR2 comprising the amino acid sequence of SEQ ID NO: 32, and a CDR3 comprising the amino acid sequence of SEQ ID NO: 14; The ISVD that binds to human serum albumin comprises a CDR1 comprising the amino acid sequence of SEQ ID NO: 30, a CDR2 comprising the amino acid sequence of SEQ ID NO: 33, and a CDR3 comprising the amino acid sequence of SEQ ID NO: 15.

[0080] Preferably, the polypeptide comprises or consists of the amino acid sequence of SEQ ID NO: 1. In a most preferred embodiment, the polypeptide consists of the amino acid sequence of SEQ ID NO: 1.

[0081] The polypeptides of the present technology preferably have at least half the binding affinity, and more preferably at least the same binding affinity, for human TNFα and human OX40L as a polypeptide consisting of the amino acid sequence of SEQ ID NO: 1, where the affinity is measured using the same method, such as Sierra SPR-32 (SPR).

[0082] 5.1 Immunoglobulin Single Variable Domains The term "immunoglobulin single variable domain" (ISVD) is used synonymously with "single variable domain" and defines an immunoglobulin molecule in which the antigen-binding site resides in, and is formed by, a single immunoglobulin domain. This sets immunoglobulin single variable domains apart from "conventional" immunoglobulins (such as monoclonal antibodies) or fragments thereof (such as Fab, Fab', F(ab')2, scFv, dis-scFv), in which two immunoglobulin domains, particularly two variable domains, interact to form the antigen-binding site. Typically, in conventional immunoglobulins, the heavy chain variable domain (V H ) and the light chain variable domain (VL ) interact to form the antigen-binding site. In this case, V H and V L Both complementarity-determining regions (CDRs) of the nucleotides contribute to the antigen-binding site, meaning that a total of six CDRs are involved in forming the antigen-binding site.

[0083] In light of the above definition, the antigen-binding domain of a conventional four-chain antibody (such as an IgG, IgM, IgA, IgD, or IgE molecule; as known in the art), or an Fv fragment such as a Fab fragment, a F(ab')2 fragment, a disulfide-linked Fv or scFv fragment, or a diabody derived from such a conventional four-chain antibody (all as known in the art) would not normally be considered an immunoglobulin single variable domain, since in such cases binding to the corresponding epitope of the antigen is usually not by one (single) immunoglobulin domain, but by a pair of (related) immunoglobulin domains, such as a light and heavy chain variable domain, i.e., the V of the immunoglobulin domains, which together bind to the epitope of the corresponding antigen. H -V L This is because it will be generated by

[0084] In contrast, an immunoglobulin single variable domain is capable of specifically binding to an epitope of an antigen without pairing with an additional immunoglobulin variable domain. The binding site of an immunoglobulin single variable domain consists of a single V H , a single V HH , or a single V L It is formed by domains.

[0085] Thus, a single variable domain may be any light chain variable domain sequence (e.g., V), as long as it is capable of forming a single antigen-binding domain (i.e., a functional antigen-binding unit consisting essentially of a single variable domain such that the single antigen-binding domain does not need to interact with another variable domain to form a functional antigen-binding unit). L sequence) or a suitable fragment thereof; or a heavy chain variable domain sequence (e.g., VH Array or V HH sequence) or a suitable fragment thereof.

[0086] Immunoglobulin single variable domains (ISVDs) include, for example, camelid-type VDs. H or humanized V HH Including V H , V HH Preferably, the immunoglobulin single variable domain (ISVD) is a camelidized V H or humanized V HH Including V HH The heavy chain ISVD may be derived from a conventional four-chain antibody or a heavy chain antibody.

[0087] For example, an immunoglobulin single variable domain may be a single domain antibody (or an amino acid sequence suitable for use as a single domain antibody), a "dAb" or dAb (or an amino acid sequence suitable for use as a dAb), or a Nanobody® (as defined herein, V HH ); other single variable domains, or any suitable fragment of any one of them.

[0088] In particular, immunoglobulin single variable domains are available as Nanobodies® (humanized V HH or Camelidization V H Contains V HH etc.) or a suitable fragment thereof. Nanobody®, Nanobodies®, and Nanoclone® are registered trademarks.

[0089] V HH , V HH antibody fragments, and V HH Also known as antibodies, "V HHThe "V domain" was originally described as the antigen-binding immunoglobulin variable domain of a "heavy chain antibody" (i.e., an "antibody lacking light chains"; Hamers-Casterman et al., Nature 363:446-448, 1993). HH The term "variable domain" refers to such a variable domain as compared to the heavy chain variable domain (herein referred to as "V" in a conventional four-chain antibody). H domain") present in conventional four-chain antibodies and the light chain variable domain (referred to herein as "V L The domain name was chosen to distinguish it from the domains known as "domains." HH For a further description of this, see the review article by Muyldermans (Reviews in Molecular Biotechnology 74:277-302, 2001).

[0090] Typically, the production of immunoglobulins involves immunizing laboratory animals, fusing immunoglobulin-producing cells to create hybridomas, and screening for the desired specificity. Alternatively, immunoglobulins can be produced by screening naive or synthetic libraries, for example, by phage display.

[0091] The generation of immunoglobulin sequences such as Nanobodies® has been extensively described in various publications, including WO 94 / 04678, Hamers-Casterman et al., 1993, and Muyldermans et al., 2001 (Reviews in Molecular Biotechnology 74:277-302, 2001). In such methods, camelids are immunized with a target antigen to induce an immune response against the target antigen. The repertoire of Nanobodies obtained from the immunization is further screened for Nanobodies that bind to the target antigen.

[0092] In such cases, the generation of antibodies requires purified antigen for immunization and / or screening. The antigen may be purified from a natural source or may be purified during recombinant production.

[0093] Immunization and / or screening for immunoglobulin sequences can be carried out using peptide fragments of such antigens.

[0094] The present technology can use immunoglobulin sequences of different origins, including mouse, rat, rabbit, donkey, human, and camelid immunoglobulin sequences. The technology also includes fully human, humanized, or chimeric sequences. For example, the technology includes camelid immunoglobulin sequences and humanized camelid immunoglobulin sequences, or camelidized domain antibodies, such as camelidized dAbs, as described in Ward et al. (See, e.g., WO 94 / 04678 and Riechmann, Febs Lett., 339:285-290, 1994, and Prot. Eng., 9:531-537, 1996). Furthermore, the technology can be used to create fused immunoglobulin sequences (one or more V) to form, for example, multivalent and / or multispecific constructs. HH For multivalent and multispecific polypeptides comprising domains and their preparation, see Conrath et al., J. Biol. Chem., Vol. 276, No. 10, pp. 7346-7350, 2001; and also, for example, WO 96 / 34103 and WO 99 / 23221), as well as immunoglobulin sequences comprising tags or other functional moieties, e.g., toxins, labels, radiochemicals, etc., derivable from the immunoglobulin sequences of the present technology, are also used.

[0095] "Humanized V HH " is a naturally occurring V HH domain, but is "humanized," i.e., HHOne or more amino acid residues of the amino acid sequence (and particularly the framework sequences) of the V H The amino acid sequence of such a humanized V domain may be obtained by substituting one or more amino acid residues present at the corresponding positions of the V domain. This can be performed in a manner known per se, and will be clear to a person skilled in the art, for example, based on the further explanations herein and the prior art (e.g., WO 2008 / 020079). HH It should be noted that the VHH domain-containing polypeptide may be obtained in any suitable manner known per se, and is therefore not strictly limited to polypeptides obtained using a naturally occurring polypeptide comprising a VHH domain as a starting material.

[0096] "Camelid V H " is a naturally occurring V H The amino acid sequence of the naturally occurring V domain corresponds to that of the V domain but has been "camelized", i.e., derived from a traditional four-chain antibody. H One or more amino acid residues of the amino acid sequence of the heavy chain antibody H The amino acid sequence of the present invention is preferably obtained by substituting one or more amino acid residues present at the corresponding positions of the V domain. This can be carried out in a manner known per se and will be clear to a person skilled in the art, for example, based on the further explanations herein and the prior art (e.g., WO 2008 / 020079). Such "camelidization" substitutions are preferably carried out by substituting one or more amino acid residues present at the corresponding positions of the V domain. H -V L The amino acid residues are inserted at amino acid positions that form and / or are present at interfaces and / or so-called camelid signature residues, as defined herein (see, e.g., WO 94 / 04678 and Davies and Riechmann (1994 and 1996), supra). H V, which is used as a starting material or starting point for generating or designing H The sequence is preferably VH V sequences derived from mammals, such as 3 sequences H sequence, more preferably human V H However, such camelid V H can be obtained in any suitable manner known per se, and thus the naturally occurring V H It should be noted that the present invention is not strictly limited to polypeptides obtained using a polypeptide containing the domain as a starting material.

[0097] It should be noted that one or more immunoglobulin sequences can be linked (e.g., by disulfide bridges) to each other and / or to other amino acid sequences to provide peptide constructs (e.g., Fab' fragments, F(ab')2 fragments, scFv constructs, "diabodies," and other multispecific constructs) that may also be useful in the present technology. See, for example, the review by Holliger and Hudson, Nat Biotechnol. 2005 Sept;23(9):1126-36. Generally, if the polypeptide is intended for administration to a subject (e.g., for prophylactic, therapeutic, and / or diagnostic purposes), the polypeptide preferably comprises an immunoglobulin sequence that does not naturally occur in the subject.

[0098] A preferred structure of an immunoglobulin single variable domain sequence can be considered to consist of four framework regions ("FR"), referred to in the art and herein as "framework region 1" ("FR1"); "framework region 2" ("FR2"); "framework region 3" ("FR3"); and "framework region 4" ("FR4"), respectively, interrupted by three complementarity-determining regions ("CDRs"), referred to in the art and herein as "complementarity-determining region 1" ("CDR1"); "complementarity-determining region 2" ("CDR2"); and "complementarity-determining region 3" ("CDR3"), respectively.

[0099] As further described in paragraph q) of pages 58 and 59 of WO 08 / 020079, the amino acid residues of immunoglobulin single variable domains are selected from the group consisting of V and VL domains derived from camelids as described by Riechmann and Muyldermans, 2000 (J. Immunol. Methods 240(1-2):185-195; see e.g., Figure 2 therein). HH As applied to the domain, V is as defined by Kabat et al. ("Sequence of proteins of immunological interest," US Public Health Services, NIH Bethesda, MD, Publication No. 91). H It can be numbered according to the domain's base numbering. H Domains and V HH It should be noted that, as is well known in the art for domains, the total number of amino acid residues in each of the CDRs may vary and may not correspond to the total number of amino acid residues indicated by the Kabat numbering (i.e., one or more positions according to the Kabat numbering may be unoccupied in the actual sequence, or the actual sequence may contain more amino acid residues than allowed by the Kabat numbering). This generally means that the Kabat numbering may or may not correspond to the actual numbering of the amino acid residues in the actual sequence. H Domains and V HH The total number of amino acid residues in a domain will usually be in the range of 110 to 120, often in the range of 112 to 115. However, it should be noted that smaller and longer sequences may also be suitable for the purposes described herein.

[0100] In this application, unless otherwise indicated, CDR sequences were determined according to the AbM numbering as described in Kontermann and Dubel (eds. 2010, Antibody Engineering, Vol. 2, Springer Verlag Heidelberg Berlin, Martin, Chapter 3, pp. 33-51). According to this method, FR1 comprises amino acid residues at positions 1-25, CDR1 comprises amino acid residues at positions 26-35, FR2 comprises amino acid residues at positions 36-49, CDR2 comprises amino acid residues at positions 50-58, FR3 comprises amino acid residues at positions 59-94, CDR3 comprises amino acid residues at positions 95-102, and FR4 comprises amino acid residues at positions 103-113.

[0101] The CDR regions can also be determined by different methods: In the Kabat CDR determination, FR1 of an immunoglobulin single variable domain comprises amino acid residues 1 to 30, CDR1 of an immunoglobulin single variable domain comprises amino acid residues 31 to 35, FR2 of an immunoglobulin single variable domain comprises amino acid residues 36 to 49, CDR2 of an immunoglobulin single variable domain comprises amino acid residues 50 to 65, FR3 of an immunoglobulin single variable domain comprises amino acid residues 66 to 94, CDR3 of an immunoglobulin single variable domain comprises amino acid residues 95 to 102, and FR4 of an immunoglobulin single variable domain comprises amino acid residues 103 to 113.

[0102] In such immunoglobulin sequences, the framework sequences may be any suitable framework sequences, and examples of suitable framework sequences will be clear to the skilled person, e.g., based on standard handbooks and the further disclosure and prior art referred to herein.

[0103] The framework sequences are preferably immunoglobulin framework sequences or (suitable combinations of) framework sequences derived from immunoglobulin framework sequences (e.g., by humanization or camelidization). For example, the framework sequences may be those of a light chain variable domain (e.g., VL sequence) and / or heavy chain variable domains (e.g., V H Array or V HH In one particularly preferred embodiment, the framework sequences are derived from V HH the framework sequences being derived from the V sequences (which may optionally be partially or fully humanized), or the conventional V sequences being camelidized. H sequences (as defined herein).

[0104] In particular, the framework sequences present in the ISVD sequences used in the present technology are such that the ISVD sequence is a humanized V HH or Camelidization V H Contains V HH Some preferred, but non-limiting examples of (suitable combinations of) such framework sequences will become clear from the further disclosure herein.

[0105] Again, any suitable fragment (or combination of fragments) of the foregoing may be used, such as a fragment comprising one or more CDR sequences suitably flanked and / or linked by one or more framework sequences (e.g., such CDR and framework sequences in the same order as they may occur in the full-sized immunoglobulin sequence from which the fragment is derived), as generally described herein with respect to immunoglobulin sequences.

[0106] However, it should be noted that the present technology is not limited with respect to the origin of the ISVD sequence (or the nucleotide sequence used to express it), nor with respect to the manner in which the ISVD sequence or nucleotide sequence is generated or obtained (or has been generated or obtained). Thus, the ISVD sequence may be a naturally occurring sequence (derived from any suitable species) or a synthetic or semi-synthetic sequence. In specific, but non-limiting, embodiments, the ISVD sequence may be a naturally occurring sequence (derived from any suitable species) or a "humanized" (as defined herein) immunoglobulin sequence (partially or fully humanized mouse or rabbit immunoglobulin sequences, and particularly partially or fully humanized VHV sequences). HH sequences), "camelized" (as described herein) immunoglobulin sequences, as well as synthetic or semi-synthetic sequences, including, but not limited to, immunoglobulin sequences obtained by techniques such as affinity maturation (e.g., starting from synthetic, random, or naturally occurring immunoglobulin sequences), CDR-grafting, veneering, combining fragments derived from different immunoglobulin sequences, PCR assembly using overlapping primers, and similar techniques for genetically engineering immunoglobulin sequences that are well known to those skilled in the art; or any suitable combination of any of the foregoing.

[0107] Likewise, the nucleotide sequence may be a naturally occurring nucleotide sequence or a synthetic or semi-synthetic sequence, for example a sequence that has been isolated by PCR from a suitable naturally occurring template (e.g. DNA or RNA isolated from a cell), a nucleotide sequence that has been isolated from a library (in particular an expression library), a nucleotide sequence that has been prepared by introducing mutations into a naturally occurring nucleotide sequence (using any suitable technique known per se, such as mismatch PCR), a nucleotide sequence that has been prepared by PCR using overlapping primers, or a nucleotide sequence that has been prepared using techniques for DNA synthesis known per se.

[0108] As mentioned above, the ISVD may be a Nanobody® or a suitable fragment thereof. For a basic description of Nanobodies, see the further description below and the prior art cited herein. However, in this regard, the present specification and the prior art will primarily focus on so-called "V H 3 Class Nanobodies (i.e., V such as DP-47, DP-51, or DP-29) H It should be noted that three classes of Nanobodies with a high degree of sequence homology to human germline sequences have been described. However, in the broadest sense, the present technology can generally use any type of Nanobody, including, for example, so-called "V" Nanobodies, as described in WO 2007 / 118670. H Nanobodies belonging to the "4th class" (i.e., V such as DP-78) H Note that four classes of Nanobodies with a high degree of sequence homology to human germline sequences can also be used.

[0109] In general, Nanobodies (especially (partially) humanized V HH Sequence and Camelidization V H V containing arrays HH Nanobodies (sequences) can be characterized by the presence of one or more "hallmark residues" (as described herein) in one or more of the framework sequences (again as further described herein). Thus, in general, Nanobodies are characterized by the (basic) structure FR1-CDR1-FR2-CDR2-FR3-CDR3-FR4 wherein FR1-FR4 refer to Framework Regions 1-4, respectively, and CDR1-CDR3 refer to Complementarity Determining Regions 1-3, respectively, and wherein one or more of the Hallmark Residues are as further defined herein.

[0110] In particular, nanobodies have the following (basic) structure: FR1-CDR1-FR2-CDR2-FR3-CDR3-FR4 wherein FR1-FR4 refer to framework regions 1-4, respectively, and CDR1-CDR3 refer to complementarity determining regions 1-3, respectively, and the framework sequences are as further defined herein.

[0111] More particularly, Nanobodies have the (basic) structure FR1-CDR1-FR2-CDR2-FR3-CDR3-FR4 wherein FR1 to FR4 refer to framework regions 1 to 4, respectively, and CDR1 to CDR3 refer to complementarity determining regions 1 to 3, respectively, and wherein one or more of the amino acid residues at positions 11, 37, 44, 45, 47, 83, 84, 103, 104, and 108 according to the Kabat numbering system are selected from the Hallmark residues set forth in Table 1 below.

[0112] [Table 1]

[0113] In this technology, ISVDs that can specifically bind to, among others, TNFα or OX40L are used. In the context of this technology, "binding" to a specific target molecule has the usual meaning in the art, as understood in the context of antibodies and their corresponding antigens.

[0114] The polypeptide of the present technology may comprise two or more ISVDs that specifically bind to TNFα and two or more ISVDs that specifically bind to OX40L. For example, the polypeptide may comprise two ISVDs that specifically bind to TNFα and two ISVDs that specifically bind to OX40L.

[0115] In some embodiments, at least one ISVD can functionally block its target molecule.For example, ISVD can block the interaction between TNFα and TNFR (TNF receptor), or can block the interaction between OX40L and OX40 (receptor), and preferably can inhibit the OX40L-induced IL2 release from T cells.Therefore, in a preferred embodiment, the polypeptide of the present technology comprises at least two ISVDs that specifically bind to TNFα and functionally block the interaction with TNFR, and two ISVDs that specifically bind to OX40L and functionally block the interaction with OX40.

[0116] The ISVDs used in the present technology form part of the polypeptides of the present technology, and the polypeptides of the present technology comprise or consist of at least four ISVDs such that the polypeptides can specifically bind to TNFα and OX40L.

[0117] Therefore, the target molecules of at least four ISVDs used in the polypeptide of the present technology are TNFα and OX40L.Examples are mammalian TNFα and OX40L.Human TNFα (Uniprot accession number P01375) and human OX40L (Uniprot accession number P23510) are preferred, but the TNFα and human OX40L from other species, for example, from non-human primates such as mouse, rat, rabbit, cat, dog, goat, sheep, horse, pig, cynomolgus monkey (also referred to herein as "cyno"), or from camelids such as llama or alpaca, can also be adapted to the present technology.

[0118] Specific examples of ISVDs that specifically bind to TNFα or OX40L that can be used in the present technology are described in sections A and B below: A. Specific binding to human OX40L. i. CDR1 comprising the amino acid sequence of SEQ ID NO: 7 or having two or one amino acid difference from SEQ ID NO: 7; ii. CDR2 comprising the amino acid sequence of SEQ ID NO: 10 or having two or one amino acid difference from SEQ ID NO: 10; and iii. A CDR3 comprising the amino acid sequence of SEQ ID NO: 13 or having two or one amino acid difference from SEQ ID NO: 13. and preferably comprising a CDR1 comprising the amino acid sequence of SEQ ID NO:7, a CDR2 comprising the amino acid sequence of SEQ ID NO:10, and a CDR3 comprising the amino acid sequence of SEQ ID NO:13.

[0119] Preferred examples of such ISVDs that specifically bind to human OX40L have one or more (preferably all) framework regions (in addition to the CDRs as defined above in section A) as shown for construct 1E07 / 1 in Table A-2. Most preferred is an ISVD that includes the complete amino acid sequence of construct 1E07 / 1 (SEQ ID NO: 2 or 3, see Tables A-1 and A-2).

[0120] Also, in preferred embodiments, the amino acid sequence of an ISVD that specifically binds to human OX40L may have greater than 90% sequence identity, such as greater than 95% or greater than 99%, with SEQ ID NO: 2 or 3, and optionally the CDRs are as defined above in section A. In particular, an ISVD that specifically binds to OX40L preferably comprises the amino acid sequence of SEQ ID NO: 2 or 3.

[0121] If such an ISVD that specifically binds to OX40L has two or one amino acid difference in at least one CDR compared to the corresponding reference CDR sequence (item A above), the ISVD preferably has at least half the binding affinity for human OX40L of construct 1E07 / 1 shown in SEQ ID NO: 2 or 3, more preferably at least the same binding affinity, where the binding affinity is measured using the same method, such as SPR.

[0122] B. Binds specifically to human TNFα i. CDR1 comprising the amino acid sequence of SEQ ID NO: 8 or having two or one amino acid difference from SEQ ID NO: 8; ii. CDR2 comprising the amino acid sequence of SEQ ID NO: 11 or having two or one amino acid difference from SEQ ID NO: 11; and iii. CDR3 comprising the amino acid sequence of SEQ ID NO: 14 or having two or one amino acid difference from SEQ ID NO: 14 and preferably, a CDR1 comprising the amino acid sequence of SEQ ID NO:8, a CDR2 comprising the amino acid sequence of SEQ ID NO:11, and a CDR3 comprising the amino acid sequence of SEQ ID NO:14.

[0123] Preferred examples of such ISVDs that specifically bind to human TNFα have one or more (preferably all) framework regions (in addition to the CDRs as defined above in section B) as shown for construct 1C02 / 1 in Table A-2. Most preferred are ISVDs that include the complete amino acid sequence of construct 1C02 / 1 (SEQ ID NO: 4 or 6, see Tables A-1 and A-2).

[0124] Also, in preferred embodiments, the amino acid sequence of an ISVD that specifically binds to human TNFα may have greater than 90% sequence identity, such as greater than 95% or greater than 99%, to SEQ ID NO: 4 or 6, and optionally the CDRs are as defined above in section B. In particular, an ISVD that specifically binds to TNFα preferably comprises the amino acid sequence of SEQ ID NO: 4 or 6.

[0125] If such an ISVD that specifically binds to TNFα has two or one amino acid difference in at least one CDR compared to the corresponding reference CDR sequence (item B above), the ISVD preferably has at least half the binding affinity for human TNFα of construct 1C02 / 1 shown in SEQ ID NO: 4 or 6, more preferably at least the same binding affinity, where the binding affinity is measured using the same method, such as SPR.

[0126] Preferably, each of the ISVDs as defined in items A and B above is included in the polypeptide of the present technology.

[0127] Such polypeptides of the present technology comprising each of the ISVDs as defined in sections A and B above preferably have at least half the binding affinity, and more preferably at least the same binding affinity, for human TNFα and human OX40L as the polypeptide consisting of the amino acid sequence of SEQ ID NO: 1, where the affinity is measured using the same methods, such as SPR.

[0128] The SEQ ID NOs referenced in sections A and B above are based on the CDR definitions according to the AbM rules (see Table A-2). However, SEQ ID NOs defining the same CDRs according to the Kabat rules (see Table A-2.1) can also be used in sections A and B above.

[0129] Thus, specific examples of ISVDs that specifically bind to TNFα or OX40L that can be used in the present technology are as described above using the AbM convention, and can also be described using the Kabat convention as shown in sections A'-B' below: A'. Specific binding to human OX40L. i. CDR1 comprising the amino acid sequence of SEQ ID NO: 28 or having two or one amino acid difference from SEQ ID NO: 28; ii. CDR2 comprising the amino acid sequence of SEQ ID NO: 31 or having two or one amino acid difference from SEQ ID NO: 31; and iii. A CDR3 comprising the amino acid sequence of SEQ ID NO: 13 or having two or one amino acid difference from SEQ ID NO: 13. and preferably comprising a CDR1 comprising the amino acid sequence of SEQ ID NO: 28, a CDR2 comprising the amino acid sequence of SEQ ID NO: 31, and a CDR3 comprising the amino acid sequence of SEQ ID NO: 13.

[0130] Preferred examples of such ISVDs that specifically bind to human OX40L have one or more (preferably all) framework regions (in addition to the CDRs as defined above in section A') as shown for construct 1E07 / 1 in Table A-2-1. Most preferred are ISVDs that include the complete amino acid sequence of construct 1E07 / 1 (SEQ ID NO: 2 or 3, see Tables A-1 and A-2-1).

[0131] B'. Binds specifically to human TNFα i. a CDR1 comprising the amino acid sequence of SEQ ID NO: 29 or having two or one amino acid difference from SEQ ID NO: 29; ii. CDR2 comprising the amino acid sequence of SEQ ID NO: 32 or having two or one amino acid difference from SEQ ID NO: 32; and iii. CDR3 comprising the amino acid sequence of SEQ ID NO: 14 or having two or one amino acid difference from SEQ ID NO: 14 and preferably comprising a CDR1 comprising the amino acid sequence of SEQ ID NO: 29, a CDR2 comprising the amino acid sequence of SEQ ID NO: 32, and a CDR3 comprising the amino acid sequence of SEQ ID NO: 14.

[0132] Preferred examples of such ISVDs that specifically bind to human TNFα have one or more (preferably all) framework regions (in addition to the CDRs as defined above in section B') as shown for construct 1C02 / 1 in Table A-2-1. Most preferred are ISVDs that include the complete amino acid sequence of construct 1C02 / 1 (SEQ ID NO: 4 or 6, see Tables A-1 and A-2-1).

[0133] The percentage of "sequence identity" between a first amino acid sequence and a second amino acid sequence can be calculated by dividing the number of amino acid residues in the first amino acid sequence that are identical to amino acid residues at corresponding positions in the second amino acid sequence by the total number of amino acid residues in the first amino acid sequence and multiplying by 100%, where each deletion, insertion, substitution, or addition of an amino acid residue in the second amino acid sequence compared to the first amino acid sequence is considered to be a difference at a single amino acid residue (i.e., a single position).

[0134] Typically, for purposes of determining the percentage of "sequence identity" between two amino acid sequences according to the calculation method outlined above, the amino acid sequence with the largest number of amino acid residues will be considered the "first" amino acid sequence, and the other amino acid sequence will be considered the "second" amino acid sequence.

[0135] An "amino acid difference," as used herein, refers to a deletion, insertion, or substitution, preferably a substitution, of a single amino acid residue relative to the reference sequence.

[0136] The amino acid substitutions are preferably conservative substitutions, preferably in which one amino acid residue within the following groups (a) to (e) is replaced with another amino acid residue within the same group: (a) small aliphatic non-polar or slightly polar residues: Ala, Ser, Thr, Pro, and Gly; (b) polar negatively charged residues and their (uncharged) amides: Asp, Asn, Glu, and Gln; (c) polar positively charged residues: His, Arg, and Lys; (d) large aliphatic non-polar residues: Met, Leu, Ile, Val, and Cys; and (e) aromatic residues: Phe, Tyr, and Trp.

[0137] Particularly preferred conservative substitutions are: Ala to Gly or Ser; Arg to Lys; Asn to Gln or His; Asp to Glu; Cys to Ser; Gln to Asn; Glu to Asp; Gly to Ala or Pro; His to Asn or Gln; Ile to Leu or Val; Leu to Ile or Val; Lys to Arg, Gln, or Glu; Met to Leu, Tyr, or Ile; Phe to Met, Leu, or Tyr; Ser to Thr; Thr to Ser; Trp to Tyr; Tyr to Trp; and / or Phe to Val, Ile, or Leu.

[0138] 5.2 Specificity The terms "specificity," "specifically binds," or "specific binding" refer to the number of different target molecules, such as antigens, from the same organism to which a particular binding unit, such as an ISVD, can bind with sufficiently high affinity (see below). "Specificity," "specifically binds," or "specific binding" are used herein synonymously with "selectivity," "selectively binds," or "selective binding." A binding unit, such as an ISVD, preferably binds specifically to a designated target.

[0139] The specificity / selectivity of a binding unit can be determined based on affinity. Affinity represents the strength or stability of a molecular interaction. Affinity is generally given by KD or dissociation constant, which has units of moles / liter (or M). Affinity is equal to 1 / KD, (moles / liter) -1 (or M -1 It can also be expressed as a binding constant, K A , which has units of kJ / kcal.

[0140] Affinity is a measure of the strength of binding between a moiety and a binding site on a target molecule: the smaller the value of KD, the stronger the binding strength between the target molecule and the targeting moiety.

[0141] Typically, the coupling units (such as ISVDs) used in this technology are 10 -5 ~10 -12moles / liter or lower, preferably 10 -7 ~10 -12 moles / liter or lower, more preferably 10 -8 ~10 -12 Dissociation constant (KD) in moles / liter (i.e., 10 5 ~10 12 Liters / mole or greater, preferably 10 7 ~10 12 liters / mole or greater, more preferably 10 8 ~10 12 It will bind to the target with an association constant (KA) in liters / mol.

[0142] 10 -4 Any KD value greater than moles / liter (or 10 4 Any K A value less than 1 / mol / liter is generally considered to indicate nonspecific binding.

[0143] The KD for biological interactions that are considered specific, such as the binding of an immunoglobulin sequence to an antigen, is typically in the range of 10 -5 moles / liter (10,000 nM or 10 μM) to 10 -12 mole / liter (0.001 nM or 1 pM) range or lower.

[0144] Therefore, specific / selective binding can be measured using the same measurement method, e.g., SPR, to determine whether the binding unit (or a polypeptide comprising it) is more than 10 -5 ~10 -12 binds to TNFα and / or OX40L with K values ​​of 10 mol / L or lower -4This can mean that the KD value of the OX40L-related marker is greater than 10 moles / liter. Examples of OX40L-related markers include human TRAIL, CD30L, CD40L, and RANKL. Examples of TNFα-related cytokines include TNF superfamily members FASL, TNFβ, LIGHT, TL-1A, and RANKL. Therefore, in an embodiment of the present technology, at least two ISVDs contained in the polypeptide are 10 -5 ~10 -12 Binds to TNFα with a KD value of 10 mol / liter or lower -4 Binds to FASL, TNFβ, LIGHT, TL-1A, and RANKL from the same species with KD values ​​greater than 10 mol / liter, and contains at least two ISVDs. -5 ~10 -12 Binds to OX40L with a KD value of 10 mol / L or lower -4 It binds to human TRAIL, CD30L, CD40L, and RANKL from the same species with K values ​​greater than 1000 mol / liter.

[0145] Thus, the polypeptide of the present technology preferably has at least half the binding affinity, and more preferably at least the same binding affinity, for human TNFα and human OX40L as the polypeptide consisting of the amino acid sequence of SEQ ID NO: 1, and the binding affinity is measured using the same method, such as SPR.

[0146] Specific binding to a specific target from a specific species does not exclude that the binding unit can also specifically bind to a similar target from a different species.For example, specific binding to human TNFα does not exclude that the binding unit (or a polypeptide comprising it) can also specifically bind to TNFα from cynomolgus monkeys.Similarly, specific binding to human OX40L does not exclude that the binding unit (or a polypeptide comprising it) can also specifically bind to OX40L from cynomolgus monkeys ("cyno").

[0147] The specific binding of a binding unit to a designated target can be determined in any suitable manner known per se, including, for example, Scatchard analysis and / or competitive binding assays, such as radioimmunoassays (RIA), enzyme immunoassays (EIA), and sandwich competition assays, as well as their different variants known per se in the art; and other techniques mentioned herein.

[0148] The dissociation constant may be an actual dissociation constant or an apparent dissociation constant, as will be apparent to those skilled in the art. Methods for determining dissociation constants will be apparent to those skilled in the art and include, for example, the techniques mentioned below. In this regard, -4 moles / liter or 10 -3 moles / liter (e.g., 10 -2 It will also be apparent that it may not be possible to measure the dissociation constant (in moles / liter). In some cases, as will be apparent to one skilled in the art, the (actual or apparent) dissociation constant can be calculated based on the (actual or apparent) binding constant (KA) according to the relationship [KD=1 / KA].

[0149] The affinity of the molecular interaction between two molecules can be determined by the well-known surface plasmon resonance (SPR) biosensor. Surface plasmon resonance can be measured by a variety of techniques known per se, including surface plasmon resonance (SPR) techniques (see, for example, Ober et al., 2001, Intern. Immunology 13:1551-1559). The term "surface plasmon resonance," as used herein, refers to an optical phenomenon that allows the analysis of biospecific interactions in real time by detecting changes in protein concentration within a biosensor matrix, where one molecule is immobilized on a biosensor chip and another molecule is passed over the immobilized molecule under flow conditions to generate k on , k off measurements, and therefore K D (or K A) values ​​are obtained. This can be performed, for example, using the well-known BIAcore® system (BIAcore International AB, a GE Healthcare company, Uppsala, Sweden, and Piscataway, New Jersey). For further description, see Jonsson et al. (1993, Ann. Biol. Clin. 51:19-26), Jonsson et al. (1991, Biotechniques 11:620-627), Johnson et al. (1995, J. Mol. Recognit. 8:125-131), and Johnson et al. (1991, Anal. Biochem. 198:268-277).

[0150] Another well-known biosensor technique for determining the affinity of biomolecular interactions is biolayer interferometry (BLI) (see, e.g., Abdiche et al., 2008, Anal. Biochem. 377:209-217). The term "biolayer interferometry" or "BLI," as used herein, refers to a label-free optical technique in which the interference pattern of light reflected from two surfaces: an internal reference layer (reference beam) and a layer of immobilized proteins on a biosensor chip (signal beam) is analyzed. Changes in the number of molecules bound to the biosensor chip cause a shift in the interference pattern, reported as a wavelength shift (nm), the magnitude of which is a direct measure of the number of molecules bound to the biosensor chip surface. Interactions can be measured in real time, allowing the determination of binding and dissociation rates and affinity. BLI can be performed, for example, using the well-known Octet® system (ForteBio, a division of Pall Life Sciences, Menlo Park, USA).

[0151] Alternatively, affinity can be measured by Kinetic Exclusion Assay (KinExA) using the KinExA® platform (Sapidyne Instruments Inc, Boise, USA) (see, e.g., Drake et al., 2004, Anal. Biochem., 328:35-43). The term "KinExA" as used herein refers to a solution-based method for measuring the true equilibrium binding affinity and kinetics of unmodified molecules. An equilibrated solution of antibody / antigen complexes is passed through a column containing beads pre-coated with the antigen (or antibody), allowing free antibody (or antigen) to bind to the coated molecules. Detection of the antibody (or antigen) captured in this manner is achieved using a fluorescently labeled protein that binds to the antibody (or antigen).

[0152] The GYROLAB® Immunoassay System provides a platform for automated bioanalysis and rapid sample turnover (Fraley et al., 2013, Bioanalysis 5:1765-74).

[0153] 5.3 (in vivo) half-life extension The polypeptide may further comprise one or more other groups, residues, moieties, or binding units, optionally linked via one or more peptide linkers, which provide the polypeptide with an increased half-life (in vivo) compared to a corresponding polypeptide that does not have one or more other groups, residues, moieties, or binding units. Increased in vivo half-life means, for example, that the polypeptide exhibits an increased half-life in a mammal, such as a human subject, after administration. Half-life can be expressed, for example, as t1 / 2beta.

[0154] The type of group, residue, moiety, or binding unit is generally not limited and can be selected from the group consisting of, for example, a polyethylene glycol molecule, a serum protein or fragment thereof, a binding unit capable of binding to a serum protein, an Fc portion, and a small protein or peptide capable of binding to a serum protein.

[0155] More specifically, the one or more other groups, residues, moieties, or binding units that provide the polypeptide with an increased half-life can be selected from the group consisting of binding units capable of binding to serum albumin, such as human serum albumin, or serum immunoglobulins, such as IgG, and are preferably binding units capable of binding to human serum albumin. The binding unit is preferably an ISVD.

[0156] For example, WO 04 / 041865 describes Nanobodies® that bind to serum albumin (particularly to human serum albumin) which can be linked to other proteins (such as one or more other Nanobodies that bind to a desired target) to increase the half-life of the protein.

[0157] WO 06 / 122787 describes a number of Nanobodies® directed against (human) serum albumin. These Nanobodies® include the Nanobody® designated Alb-1 (SEQ ID NO: 52 in WO 06 / 122787) and its humanized variants such as Alb-8 (SEQ ID NO: 62 in WO 06 / 122787). Again, they can be used to extend the half-life of therapeutic proteins and polypeptides and other therapeutic entities or moieties.

[0158] Furthermore, WO 2012 / 175400 describes a further improved version of Alb-1, called Alb-23.

[0159] In a preferred embodiment, the polypeptide comprises a serum albumin binding moiety selected from Alb-1, Alb-3, Alb-4, Alb-5, Alb-6, Alb-7, Alb-8, Alb-9, Alb-10, and Alb-23, preferably Alb-8 or Alb-23, or a variant thereof, as set out on pages 7 to 9 of WO 2012 / 175400, and the serum albumin binders described in WO 2012 / 175741, WO 2015 / 173325, WO 2017 / 080850, WO 2017 / 085172, WO 2018 / 104444, WO 2018 / 134235, WO 2018 / 134234. Some preferred serum albumin binders are also shown in Table A-4. Particularly preferred additional components of the polypeptides of the present technology are as described in Section C: C. binds to human serum albumin i. CDR1 comprising the amino acid sequence of SEQ ID NO: 9 or having two or one amino acid difference from SEQ ID NO: 9; ii. CDR2 comprising the amino acid sequence of SEQ ID NO: 12 or having two or one amino acid difference from SEQ ID NO: 12; and iii. CDR3 comprising the amino acid sequence of SEQ ID NO: 15 or having two or one amino acid difference from SEQ ID NO: 15 and preferably comprising a CDR1 comprising the amino acid sequence of SEQ ID NO:9, a CDR2 comprising the amino acid sequence of SEQ ID NO:12, and a CDR3 comprising the amino acid sequence of SEQ ID NO:15.

[0160] A preferred example of such an ISVD that binds to human serum albumin has one or more (preferably all) framework regions (in addition to the CDRs as defined above in section C) as shown for construct ALB23002 in Table A-2. Most preferred is an ISVD that includes the complete amino acid sequence of construct ALB23002 (SEQ ID NO:5, see Tables A-1 and A-2).

[0161] Item C can also be written using the Kabat Rule as follows: C'. binds to human serum albumin i. a CDR1 comprising the amino acid sequence of SEQ ID NO: 30 or having two or one amino acid difference from SEQ ID NO: 30; ii. CDR2 comprising the amino acid sequence of SEQ ID NO: 33 or having two or one amino acid difference from SEQ ID NO: 33; and iii. CDR3 comprising the amino acid sequence of SEQ ID NO: 15 or having two or one amino acid difference from SEQ ID NO: 15 and preferably comprising a CDR1 comprising the amino acid sequence of SEQ ID NO: 30, a CDR2 comprising the amino acid sequence of SEQ ID NO: 33, and a CDR3 comprising the amino acid sequence of SEQ ID NO: 15.

[0162] A preferred example of such an ISVD that binds to human serum albumin has one or more, preferably all, framework regions (in addition to the CDRs as defined above in section C') as shown for construct ALB23002 in Table A-2.1. Most preferred is an ISVD that includes the complete amino acid sequence of construct ALB23002 (SEQ ID NO: 5, see Tables A-1 and A-2.1).

[0163] Also, in preferred embodiments, the amino acid sequence of the ISVD that binds human serum albumin may have greater than 90% sequence identity, such as greater than 95% or greater than 99%, with SEQ ID NO: 5, and optionally the CDRs are as defined above in section C. In particular, the ISVD that binds human serum albumin preferably comprises the amino acid sequence of SEQ ID NO: 5.

[0164] If such an ISVD that binds to human serum albumin has two or one amino acid difference in at least one CDR compared to the corresponding reference CDR sequence (item C above), the ISVD will have at least half the binding affinity, and preferably at least the same binding affinity, for human serum albumin as construct ALB23002 shown in SEQ ID NO: 5, where the binding affinity is measured using the same method, such as SPR.

[0165] Such an ISVD that binds to human serum albumin, when occupying the C-terminal position, exhibits a C-terminal alanine (A) or glycine (G) stretch and is preferably selected from SEQ ID NOs: 46, 47, 49, 51, 52, 53, 54, 55, 56, and 58 (see Table A-4 below). In a preferred embodiment, the ISVD that binds to human serum albumin occupies a position other than the C-terminal position (i.e., is not the C-terminal ISVD of the polypeptides of the present technology) and is selected from SEQ ID NOs: 5, 44, 45, 48, and 50 (see Table A-4 below).

[0166] 5.4 Nucleic acid molecules Nucleic acid molecules encoding the polypeptides of the present technology are also provided.

[0167] A "nucleic acid molecule" (used interchangeably with "nucleic acid") is a chain of nucleotide monomers linked together via a phosphate backbone to form a nucleotide sequence. The nucleic acid can be used, for example, to transform / transfect a host cell or host organism for expression and / or production of a polypeptide. Suitable hosts or host cells for production purposes will be apparent to those skilled in the art and may be, for example, any suitable fungal, prokaryotic, or eukaryotic cell or cell line, or any suitable fungal, prokaryotic, or eukaryotic organism. Hosts or host cells comprising nucleic acids encoding the polypeptides of the present technology are also encompassed by the present technology.

[0168] Nucleic acid can be, for example, DNA, RNA, or their hybrid, and can also contain (for example, chemically) modified nucleotides such as PNA.Nucleic acid can be single-stranded or double-stranded, and preferably in the form of double-stranded DNA.For example, the nucleotide sequence of this technology can be genomic DNA or cDNA.

[0169] The nucleic acid of the present technology can be prepared or obtained in a manner known per se, and / or can be isolated from a suitable natural source.The nucleotide sequence encoding a naturally occurring (poly)peptide can be subjected to site-directed mutagenesis, for example, to provide a nucleic acid molecule encoding a polypeptide with sequence variation.In addition, as will be clear to those skilled in the art, to prepare nucleic acid, several nucleotide sequences, such as at least one nucleotide sequence encoding a targeting moiety and the nucleic acid encoding one or more linkers, can be linked together in a suitable manner.

[0170] Techniques for generating nucleic acids will be apparent to those skilled in the art and include, but are not limited to, automated DNA synthesis; site-directed mutagenesis; combining two or more naturally occurring and / or synthetic sequences (or two or more portions thereof), introducing mutations that lead to the expression of truncated expression products; introducing one or more restriction sites (e.g., to create cassettes and / or regions that can be easily digested and / or ligated using suitable restriction enzymes), and / or introducing mutations by PCR reactions using one or more "mismatched" primers.

[0171] 5.5 Vectors Also provided is a vector that comprises the nucleic acid molecule that encodes the polypeptide of the present technology.As used herein, vector is the vehicle that is suitable for carrying genetic material into cell.Vector includes naked nucleic acid such as plasmid or mRNA, or the nucleic acid that is embedded in larger structure such as liposome or virus vector.

[0172] A vector generally comprises at least one nucleic acid, optionally operably linked to one or more regulatory elements, such as, for example, one or more suitable promoters, enhancers, terminators, etc. The vector is preferably an expression vector, i.e., a vector suitable for expressing an encoded polypeptide or construct under suitable conditions, for example, when the vector is introduced into a (e.g., human) cell. In the case of a DNA-based vector, this usually includes the presence of elements for transcription (e.g., a promoter and polyA signal) and translation (e.g., a Kozak sequence).

[0173] Preferably, in a vector, at least one nucleic acid and a regulatory element are "operably linked" to each other, which generally means that they are in a functional relationship with each other. For example, a promoter is considered to be "operably linked" to a coding sequence if it can initiate or otherwise control / regulate the transcription and / or expression of the coding sequence (in which case, the coding sequence should be understood to be "under the control" of the promoter). Generally, when two nucleotide sequences are operably linked, they will be in the same direction and usually in the same reading frame. They will also usually be essentially contiguous, although this may not be necessary.

[0174] Preferably, any regulatory elements of the vector are capable of providing their intended biological function in the intended host cell or host organism.

[0175] For example, a promoter, enhancer, or terminator must be "operable" in the intended host cell or host organism, meaning, for example, that a promoter must be capable of initiating or otherwise controlling / regulating the transcription and / or expression of a nucleotide sequence, e.g., a coding sequence, to which it is operably linked.

[0176] 5.6 Composition The present technology also provides a composition comprising at least one polypeptide of the present technology, at least one nucleic acid molecule encoding the polypeptide of the present technology, or at least one vector comprising such nucleic acid molecule.The composition is preferably a pharmaceutical composition.The composition may further comprise at least one pharmaceutically acceptable carrier, diluent or excipient, and / or adjuvant, and may optionally comprise one or more additional pharmaceutically active polypeptides and / or compounds.

[0177] 5.7 Host organisms The present technology also relates to host cells or host organisms comprising a polypeptide of the present technology, a nucleic acid encoding a polypeptide of the present technology, and / or a vector comprising a nucleic acid molecule encoding a polypeptide of the present technology.

[0178] Suitable host cells or host organisms are readily apparent to those skilled in the art, and include, for example, any suitable fungal, prokaryotic, or eukaryotic cell or cell line, or any suitable fungal, prokaryotic, or eukaryotic organism. Specific examples include HEK293 cells, CHO cells, Escherichia coli, and Pichia pastoris. The most preferred host is Pichia pastoris.

[0179] 5.8 Polypeptide Methods and Uses The present technology also provides a method for producing the polypeptide of the present technology, which may include transforming / transfecting a host cell or host organism with a nucleic acid encoding the polypeptide, expressing the polypeptide in the host, optionally followed by one or more isolation and / or purification steps. Specifically, the method includes: a. expressing a nucleic acid sequence encoding the polypeptide in a suitable host cell or host organism or in another suitable expression system; optionally followed by: b) isolating and / or purifying the polypeptide may also include:

[0180] Suitable hosts or host cells for production purposes will be apparent to those skilled in the art and may be, for example, any suitable fungal, prokaryotic, or eukaryotic cell or cell line, or any suitable fungal, prokaryotic, or eukaryotic organism. Specific examples include HEK293 cells, CHO cells, Escherichia coli, and Pichia pastoris. The most preferred host is Pichia pastoris.

[0181] The polypeptides of the present technology, the nucleic acid molecules or vectors as described herein, or compositions comprising the polypeptides, nucleic acid molecules, or vectors of the present technology, preferably the polypeptides or compositions comprising them, are useful as medicaments.

[0182] Thus, the present technology provides a polypeptide of the present technology, a nucleic acid molecule or vector as described herein, or a composition comprising a polypeptide, nucleic acid molecule, or vector of the present technology for use as a medicament.

[0183] Also provided are polypeptides of the present technology, nucleic acid molecules or vectors as described herein, or compositions comprising the polypeptides, nucleic acid molecules, or vectors of the present technology for use in the treatment (prophylactic or therapeutic) of autoimmune or inflammatory diseases.

[0184] Further provided is a method for treating (prophylactic and / or therapeutic) an autoimmune or inflammatory disease, comprising administering to a subject in need thereof a pharmaceutically active amount of a polypeptide of the present technology, a molecule or vector as described herein, or a composition comprising a polypeptide, nucleic acid molecule, or vector of the present technology.

[0185] Further provided is the use of a polypeptide of the present technology, a nucleic acid molecule or vector as described herein, or a composition comprising the polypeptide, nucleic acid molecule, or vector of the present technology in the preparation of a pharmaceutical composition, preferably for treating an autoimmune or inflammatory disease.

[0186] Autoimmune or inflammatory diseases are, for example, rheumatoid arthritis; inflammatory bowel diseases such as Crohn's disease and ulcerative colitis; psoriasis, hidradenitis suppurativa; and graft-versus-host disease.

[0187] "Subject" when referred to in the context of the present technology can be any animal, preferably a mammal.Among mammals, human and non-human mammals can be distinguished.Non-human animals can be, for example, companion animals (for example, dogs, cats), livestock (for example, cattle, horses, sheep, goats, or pigs), or animals that are generally used for research purposes and / or antibody production (for example, mice, rats, rabbits, cats, dogs, goats, sheep, horses, pigs, non-human primates such as cynomolgus monkeys, or camelids such as llamas or alpacas).

[0188] In the context of prophylactic and / or therapeutic purposes, the subject may be any animal, more particularly any mammal, but is preferably a human subject.

[0189] Substances (including polypeptides, nucleic acid molecules, and vectors) or compositions can be administered to a subject by any suitable route of administration, for example, enteral (such as oral or rectal) or parenteral (such as epicutaneous, sublingual, buccal, nasal, intraarticular, intradermal, intramuscular, intraperitoneal, intravenous, subcutaneous, transdermal, or transmucosal) administration. Parenteral administration, such as intramuscular, subcutaneous, or intradermal administration, is preferred. Subcutaneous administration is most preferred.

[0190] An effective amount of a polypeptide, a nucleic acid molecule or vector as described herein, or a composition comprising the polypeptide, nucleic acid molecule, or vector can be administered to a subject to provide the intended therapeutic result.

[0191] One or more doses can be administered. If more than one dose is administered, the doses can be administered at suitable intervals to maximize the effect of the polypeptide, composition, nucleic acid molecule, or vector.

[0192] [Table 2]

[0193] [Table 3]

[0194] [Table 4]

[0195] [Table 5]

[0196] [Table 6]

[0197] [Table 7]

[0198] 6. Working Example 6.1 Example 1: Multispecific ISVD Construct Generation Anti-TNFα V HH Building blocks (TNF06C11 (WO 2017081320), TNF01C02 (WO 2015173325, SEQ ID NO: 327), and VHH#3 (WO 2004041862)), anti-OX40L V HH building blocks (OX40L1E07, OX40L1B11, and OX40L15B07, see WO 2011073180), and anti-HSA V HH The identification of the ISVD-containing polypeptide F027300252 (SEQ ID NO: 1), which binds to TNFα and OX40L, and which included the building block ALB23002 (see WO2017134234, SEQ ID NO: 10 / WO2018131234), resulted from a data-driven multispecific engineering and formatting campaign. Various building block positions / orientations and various linker lengths (9GS, 20GS vs. 35GS) were applied, which demonstrated that these influence various parameters (potency, cross-reactivity, expression, etc.). ) has proven important for the inhibition of TNFα-induced NFκB activation and OX40L-induced T cell costimulation in vitro, as assayed in Examples 7 and 9.

[0199] A panel of 84 constructs (Table 2) was transformed into Pichia pastoris for small-scale production. Induction of ISVD construct expression occurred by the stepwise addition of methanol. Clarified medium containing the secreted ISVD constructs was used as the starting material for purification by protein A affinity chromatography followed by desalting. The purified sample was used for functional characterization and expression evaluation.

[0200] [Table 8-1] [Table 8-2]

[0201] Some constructs showed loss of potency depending on the valency, linker length, and relative position of the ISVD building blocks. For example, significant differences in OX40L potency were observed among six bispecific ISVD constructs, despite containing the same building blocks targeting OX40L and TNFα. The precise composition (valency, building block orientation, and linker length usage) was found to be important for potency. The listed OX40L blocking potencies, as shown in Table 3, demonstrate the importance of the bivalency and N-terminal position of the anti-OX40L 1E07 / 1 building block.

[0202] [Table 9]

[0203] This large panel was then reduced to a panel of five multispecific constructs consisting of ISVD constructs F027300252, F027301140, F027301189, F027301197, and F027301199, which were found to be potent against both targets (human and cynomolgus monkey) and contained the potential for high expression levels based on preliminary yield estimates.

[0204] Large-scale 2L and 5L production runs of a panel containing five ISVD constructs were performed in Pichia pastoris to determine expression yields, evaluate biophysical properties, and characterize their reactivity. It was demonstrated that a specific combination of anti-OX40L and anti-TNFα building blocks was required to achieve high expression yields and sufficient solubility and biophysical stability in Pichia pastoris. As illustrated in Table 5, where ISVD constructs F027300252 and F07301199 are compared, the use of anti-TNF building blocks resulted in significantly different CMC profiles. In 5L fermentations, ISVD construct F027300252 not only reached a titer of 6 g / L, three-fold higher than ISVD construct F07301199, but also exhibited superior storage properties and viscosity.

[0205] [Table 10]

[0206] [Table 11]

[0207] Furthermore, Table 6 and Example 12 demonstrate that pre-existing antibody reactivity is driven by the composition, valency, and linker length of each ISVD construct.

[0208] [Table 12]

[0209] Finally, ISVD construct F027300252 was selected based on potency, reduced binding to existing antibodies, excellent expression levels and CMC properties, and reduced binding to existing antibodies.

[0210] 6.2 Example 2: Binding Affinities of Multispecific ISVD Constructs for TNFα, OX40L, and Serum Albumin The equilibrium dissociation constants (K ) of F027300252 for human, cynomolgus monkey, guinea pig, and mouse TNFα, human and cynomolgus monkey OX40L, and human and cynomolgus monkey serum albumin were calculated. D Affinities, expressed as ) were quantified by in-solution affinity measurements on a Gyrolab xP workstation (Gyros).

[0211] K D Control measurements included serial dilutions of TNFα or OX40L (ranging from 1 μM to 0.1 pM) or serum albumin (ranging from 10 μM to 1 pM), and a constant amount of F027300252 (20 pM for TNFα, 30 pM for OX40L, and 10 pM for serum albumin). The antibodies were mixed with the OX40L and TNFα antibodies (300 pM for serum albumin) to allow interaction and incubated for either 24 or 48 hours (for OX40L and TNFα) or 2 hours (for serum albumin) until equilibrium was reached.

[0212] For receptor-controlled assays, serial dilutions of TNFα or OX40L (ranging from 1 μM to 0.1 pM) and a fixed amount of F027300252 (5 nM for TNFα and 5 nM for OX40L) were mixed and allowed to interact, then incubated for either 24 or 48 h until equilibrium was reached.

[0213] Biotinylated human TNFα / OX40L / serum albumin was captured on a Gyrolab Bioaffy 1000CD microstructure, which contains a column of beads and is used as a molecular probe to capture free F027300252 from the equilibrated solution. A mixture of TNFα / OX40L / serum albumin and F027300252 (including free TNFα / OX40L / serum albumin, free F027300252, and TNFα / OX40L / serum albumin-F027300252 complexes) was allowed to flow through the beads, and a small percentage of free F027300252 proportional to the free ISVD construct concentration was captured. Then, fluorescently labeled anti-v HHAntibody ABH0086-Alexa647 was injected to label all captured F027300252, and the change in fluorescence was measured after washing away excess fluorescent probe. Gyrolab Analysis software was used to fit the dilution series and calculate the K D Analyzing the control curve and receptor control curve D value was determined.

[0214] The results (Table 7) demonstrate that the multispecific ISVD construct binds with high affinity to human / cynomolgus OX40L and human / cynomolgus TNFα.

[0215] [Table 13]

[0216] 6.3 Example 3: Multispecific ISVD construct binding to membrane-bound TNFα Binding of F027300252 to membrane-bound TNFα was demonstrated using flow cytometry on human membrane TNFα-expressing HEK293H cells and on activated CD4+ cells isolated from PBMCs and stimulated with PMA and ionomycin (data shown for TNFα-expressing HEK293H cells). Briefly, 1×10 cells were cultured. 4 Cells were seeded at a density of 1000 cells / well and incubated with a dilution series of F027300252 or reference compound anti-hTNFα mAb starting at 100 nM down to 0.5 pM for 1 hour at 4°C. In parallel, cells were fixed with 4% paraformaldehyde and 0.1% glutaraldehyde in PBS before seeding (to increase detection of membrane-bound TNFα) and incubated with a dilution series of ISVD constructs or reference compounds for 1 hour at 4°C or for 24 hours at room temperature. Cells were washed three times and then incubated with anti-v HHCells were incubated with mAb (ABH00119) for 30 minutes at 4°C, washed again, and then incubated with goat anti-mouse or anti-human PE-labeled antibodies for 30 minutes at 4°C. Samples were washed and resuspended in FACS buffer (D-PBS with 10% FBS and 0.05% sodium azide supplemented with 5 nM TOPRO3). Cell suspensions were then analyzed using iQuescreener. EC50 values ​​were calculated using GraphPad Prism. After 1 hour of incubation, the EC50 values ​​of F027300252 and the anti-hTNFα reference mAb were in the same range for live and fixed cells, but fixing the cells resulted in higher levels of TNFα at the membrane (Table 8). After 24 hours of incubation, binding equilibrium was reached. The affinities of F027300252 and the anti-hTNFα reference mAb were comparable.

[0217] [Table 14]

[0218] 6.4 Example 4: Multispecific ISVD Construct Binding to Membrane-Bound OX40L Binding of F027300252 to membrane-bound human and cynomolgus OX40L was demonstrated using flow cytometry on CHO-KI cells expressing human or cynomolgus OX40L. Briefly, cells were fixed with 4% paraformaldehyde and 0.1% glutaraldehyde in PBS and incubated at 1 × 10 4 Cells were seeded at a density of 1000 cells / well and incubated with a dilution series of ISVD construct F027300252 or a reference compound anti-hTNFα mAb starting from 100 nM down to 0.5 pM for 48 hours at room temperature. Cells were washed three times and then incubated with anti-V HHThe cells were incubated with the mAb for 30 minutes at 4°C, washed again, and incubated with goat anti-mouse PE- or FITC-labeled antibody for 30 minutes at 4°C. The samples were washed and resuspended in FACS buffer (D-PBS with 10% FBS and 0.05% sodium azide supplemented with 5 nM TOPRO3). The cell suspensions were then analyzed using iQuescreener. EC50 values ​​were calculated using GraphPad Prism. F0273000252 shows greater than 10-fold better binding to membrane-bound OX40L than the reference compound anti-hOX40L reference mAb (Table 9).

[0219] [Table 15]

[0220] 6.5 Example 5: Multispecific ISVD constructs selectively bind TNFα and OX40L The absence of binding to TNFα and OX40L-related human targets was assessed by SPR (Proteon XPR36). Human TRAIL, CD30L, CD40L, and RANKL were evaluated as OX40L-related targets. Human TNF superfamily members FASL, TNFβ, LIGHT, TL-1A, and RANKL were tested as cytokines related to TNFα.

[0221] For this purpose, TNF-related cytokines were immobilized at 25 μg / mL on a Proteon GLC sensor chip using amine coupling for 200 s, followed by an 80 s injection of EDC / NHS for activation and a 150 s injection of 1 M ethanolamine HCl for deactivation (Proteon Amine Coupling Kit, catalog no. 176-2410). The flow rate during activation, deactivation, and ligand injection was set at 30 μl / min. The pH of the 10 mM acetate immobilization buffer was chosen by subtracting approximately 1.5 from the pI of each ligand.

[0222] Next, 10 nM or 300 nM F027300252 was injected for 2 min, followed by 900 s of dissociation at a flow rate of 45 μL / min. PBS (pH 7.4) + 0.005% Tween 20 was used as the running buffer. As positive controls, 0.3 μM α-hFASL Ab, 0.3 μM α-hTNFβ Ab, 0.5 μM α-hLIGHT Ab, and 0.3 μM α-hTL-1A Ab were injected. The interaction of F027300252 and the positive controls with the immobilized target was measured by detecting the increase in refractive index resulting from the change in the tip mass upon binding.

[0223] For OX40L-related targets, ISVD construct F027500252 or positive control antibodies α-hTRAIL, α-hCD30L, α-hCD40L, and α-hRANKL V HH was immobilized on the sensor chip at 10 μg / ml.

[0224] Next, 1 μM human TRAIL, CD30L, CD40L, and RANKL were injected for 2 min and allowed to dissociate for 900 s at a flow rate of 45 μL / min.

[0225] All positive controls bound to their corresponding targets. No binding of ISVD construct F027300252 to human TRAIL, CD30L, CD40L, FASL, TNFβ, LIGHT, TL-1A, or RANKL was detected.

[0226] 6.6 Example 6: Simultaneous binding of multispecific ISVD constructs to hOX40L, hTNFα, and HSA We used a Biacore T200 instrument to determine whether the ISVD construct F0273000252 could simultaneously bind recombinant soluble hTNFα and hOX40L. To this end, HSA was immobilized to a CM5 sensor chip via amine coupling to a level of 6000 RU. To capture the ISVD construct via the ALB23002 building block, 100 nM F0273000252 was injected over the HSA surface at 10 μl / min for 2 min. Subsequently, 100 nM hOX40L, hTNFα, or hIL13, or a mixture of 100 nM OX40L + 100 nM TNFα, 100 nM IL13 + 100 nM OX40L, or 100 nM TNFα + 100 nM IL13, was injected at a flow rate of 45 μl / min for 2 min, followed by a 600-second dissociation step. The HSA surface was regenerated by injecting HCl (100 mM) at 45 μl / min for 2 min. The sensorgrams (Figure 1) demonstrate that ISVD construct F027300252 can simultaneously bind hOX40L and hTNFα, as shown by the increase in response units after capture on HSA: an increase of approximately 1770 RU from hTNFα alone, an increase of approximately 800 RU from hOX40L alone, and an increase of approximately 2300 RU for the mixture of OX40L and TNFα.

[0227] Flow cytometry was used to determine whether ISVD construct F0273000252 can simultaneously bind recombinant soluble hTNFα and cell membrane-bound hOX40L. To this end, CHO-KI cells expressing human OX40L were cultured at 5 × 10 4Cells were seeded at a density of 1000 cells / well and incubated with 100 nM of the ISVD construct F027300252 for 90 min at 4°C. The mixture was then incubated with a dilution series of biotinylated TNFα, starting from 500 nM to 7.6 pM, and incubated for 30 min at 4°C in the presence of 30 μM HSA. The cells were washed three times, then incubated with PE-labeled anti-streptavidin for 30 min at 4°C, and washed again. The samples were washed and resuspended in FACS buffer (D-PBS with 10% FBS and 0.05% sodium azide supplemented with 5 nM TOPRO3). The cell suspension was then analyzed using an iQuescreener. The dose-response curve (Figure 2) showed that the ISVD construct F027300252 was able to simultaneously bind to membrane-bound hOX40L and soluble hTNFα in the presence of HSA, whereas the negative control V HH It was demonstrated that IRR0096, which is a nucleotide analog, is unable to bind.

[0228] 6.7 Example 7: In vitro inhibition of TNFα-induced NFκB activation by multispecific ISVD constructs HEK293_NFκB-NLucP cells are TNF receptor-expressing cells stably transfected with a reporter construct encoding nanoluciferase under the control of an NFκB-dependent promoter. Incubation of cells with soluble human and cynomolgus monkey TNFα resulted in NFκB-mediated nanoluciferase gene expression. Nanoluciferase luminescence was measured using Nano-Glo luciferase substrate mixed with lysis buffer and added to cells at a 1:50 ratio. Samples were mixed on a shaker for 5 minutes to obtain complete lysis.

[0229] Glo response™ HEK293_NFκB-NLucP cells were seeded at 20,000 cells / well in normal growth medium in white tissue culture (TC)-treated 96-well plates with clear bottoms. Serial dilutions of F0273000252 or a reference compound (anti-hTNFα mAb) were added to 25 pM human or 70 pM cynomolgus TNFα and incubated with the cells for 5 hours at 37°C in the presence of 30 μM HSA.

[0230] F027300252 inhibited human and cynomolgus monkey TNFα-induced NFκB activation in a concentration-dependent manner, with IC50 values ​​of 31 pM (for human TNFα) and 91 pM (for cynomolgus monkey TNFα), comparable to the reference compound anti-hTNFα mAb (Table 10, Figure 3). HH IRR00096, showed no inhibition.

[0231] [Table 16]

[0232] 6.8 Example 8: Inhibition of TNFα by multispecific ISVD constructs reduces luciferase expression in a stable NFκB luciferase reporter cell line Mono- or multispecific antibodies or ISVD constructs / V HH To measure TNFα neutralization by NFκB, we used the NFκB reporter stable cell line A549 / NFκB-luc (catalog no. RC002). Nuclear factor kappa B (NFκB) is a member of the rel family of transcription factors and plays an important role in regulating inflammatory responses, apoptosis, and tumorigenesis. The cell line used here is derived from human lung cancer cell line A549, and chromosomal integration of the luciferase reporter construct is regulated by six copies of the NFκB response element. Using this cell line, we can accurately monitor any changes occurring along the NFκB pathway.

[0233] The potency of the ISVD constructs was determined by neutralizing human TNFα (SIGMA #H8916) and cynomolgus TNFα (Sino 90018-CNAE-5) at EC90 in a dose response of 10 serially diluted concentrations. Human TNFα was used at [15 ng / ml] and cynomolgus TNFα was used at [10 ng / ml].

[0234] Thawed A549 / NFκB-luc cells were resuspended in RPMI medium containing 1% FCS and seeded into 384-well plates with 10 μl of 10K cells per well. 10 μl of anti-TNF / anti-OX40L multispecific ISVD construct F027300252, or corresponding positive and negative control antibodies, and V HH The antibody produced in-house (anti-TNFα mAb2) was used as a positive control. HH IRR00119 and antibody RA11093885 were used as negative controls. After 15 minutes of preincubation at room temperature, 10 μl of TNFα at a concentration of 15 ng / ml was added to the wells. After 5 hours at 37°C, 5% CO2, and 95% humidity, the entire reaction was terminated by adding 20 μl of Bio-Glo luciferase detection reagent (Promega E7940). Luminescence signals were measured with a PheraStar (BMG). Speed's XLfit program was used for dose-response curve fitting and IC50 value calculation.

[0235] [Table 17]

[0236] 6.9 Example 9: In Vitro Inhibition of OX40L-Induced T Cell Costimulation by Multispecific ISVD Constructs The functional activity of human and cynomolgus monkey OX40L and its inhibition by the ISVD construct F027300252 were studied using a cell-based assay to investigate OX40L-induced T cell costimulation (PBMC activity assay). The assay was performed using buffy coat-derived PBMCs (1 x 10 5 OX40L-overexpressing CHO-KI cells (1 × 10 cells / well density) were cultured in the presence of a suboptimal concentration of PHA-L (to induce OX40 expression). 4This was performed by co-culturing cells in clear 96-well plates with 100% IgG4-positive cells (density: 1000 cells / well). Serial dilutions of the ISVD construct F027300252 or the reference compound anti-hOX40L mAb were added to the co-cultures and incubated in the presence of 30 μM HSA at 37° C. in a humidified incubator for 22 hours. IL2 levels in the supernatants of these cells were assessed using ELISA for reading.

[0237] ISVD construct F027300252 inhibited human and cynomolgus OX40L-induced T cell activation in a concentration-dependent manner with IC50 values ​​of 2.58 nM (for human OX40L) and 7.22 nM (for cynomolgus OX40L), comparable to the reference compound anti-hOX40L mAb (Table 12, Figure 4).

[0238] [Table 18]

[0239] 6.10 Example 10: Inhibition of TNFα and OX40L by the multispecific ISVD construct F027300252 reduces luciferase expression in a stable NFκB luciferase reporter cell line Mono- or multispecific antibodies or ISVD constructs / V HHTo measure the neutralization of TNFα and OX40L by NFκB, individually or in combination, we used the NFκB reporter stable cell line Jurkat NFκB-Luc2 / OX40. Nuclear factor kappa B (NFκB) is a member of the rel family of transcription factors and plays an important role in regulating inflammatory responses, apoptosis, and tumorigenesis. The cell line used here was derived from human peripheral blood T lymphocytes, and chromosomal integration and stable expression of the human OX40 receptor and codon-optimized firefly luciferase reporter gene luc2 construct was regulated by six copies of the NFκB response element. This cell line allows precise monitoring of any changes occurring along the NFκB pathway. Thawed Jurkat / NFκB-Luc2 / OX40 cells were resuspended in RPMI medium containing 1% FCS and 1 x 10 cells were added to each well. 5 The cells were seeded and cultured in 96-well plates with 1000 cells / ml.

[0240] At the start of the assay, recombinant human TNFα and human OX40L were added to wells of a 96-well Eppendorf suspension culture plate at 85 μl / well to a final concentration of 5 ng / ml and 100 ng / ml, respectively, along with 85 μl of pre-diluted anti-TNFα antibody PB03017 (from Sanofi), anti-OX40L antibody (catalog no. AB00536, from Absolute Antibody), IgG1 isotype negative control antibody (catalog no. 403502, from Biolegend), and negative control V HH IRR00119 (from Sanofi), monospecific anti-TNFα V HH ATN-103 (from Sanofi), monospecific anti-OX40L V HH ALX-0632 (from Sanofi), or anti-TNF / anti-OX40L multispecific ISVD constructs F027300252, F027301104, F027301189, F027301197, and F027301199 (all from Sanofi) were added. After a 15-minute preincubation at 37°C, 75 μl of the mixture was added to 50 μl of 1 × 10 5Cells / well were added to wells and incubated for 6 hours at 37°C with 5% CO2 and 95% humidity. The reaction was stopped by adding 125 μl of Bio-Glo Luciferase Detection Reagent (Promega E7940), and the luminescence signal was measured. Speed's XLfit program was used to fit the dose-response curves and calculate IC50 values ​​in Figure 7.

[0241] The additive effect of the anti-TNFα and anti-OX40L combination compared to the efficacy of the individual arms was measured at antibody doses of 0.5 μg / ml, 1 μg / ml, 2 μg / ml, and 5 μg / ml (Figure 5). Recombinant human TNFα (catalog no. H8916, from Sigma) and recombinant human OX40L (catalog no. 71185, from bpsbioscience) were neutralized at their EC90s to achieve luciferase induction levels comparable to those of each individual stimulus, demonstrating the efficacy of monospecific V HH The IC50 (Figure 7, Table 13) and % inhibition (Figure 6, Table 13) of the multispecific ISVD constructs compared to human TNFα were measured at a dose response of nine serially diluted concentrations (see Figure 5). Human TNFα was used at 5 ng / ml and human OX40L was used at a final assay concentration of 100 ng / ml, respectively.

[0242] Incubation with both recombinant human TNFα (hTNFα) and recombinant human OX40L (OX40L) leads to a much stronger induction of luciferase activity (>3-fold) compared to treatment with either stimulus alone (see Figure 5). Using incubation with both recombinant human TNFα and recombinant human OX40L (hTNFα + OX40L), we characterized the effects of inhibition by anti-TNFα antibody alone, anti-OX40L antibody alone, or the combination of anti-TNFα and anti-OX40L antibodies at various concentrations ranging from 0.5 μg / ml to 5 μg / ml (Figure 5). Although treatment with anti-TNFα or anti-OX40L alone resulted in some inhibition of luciferase activity induced by the hTNFα+OX40L combination, only treatment with the anti-TNFα / anti-OX40L combination was able to very strongly suppress luciferase activity to a very low level corresponding to the unstimulated control (Fig. 5). Thus, combined treatment with anti-TNF / anti-OX40L, even at very low concentrations, e.g., 0.5 μg / ml or 1 μg / ml, significantly more strongly suppressed luciferase activity than treatment with anti-TNFα or anti-OX40L alone at a high concentration of 5 μg / ml (Fig. 5).

[0243] Similarly, monospecific anti-OX40L V at various concentrations ranging from 1 pM to 20 nM HH ALX-0632, or monospecific anti-TNFα V HH To characterize the effects of inhibition by ATN-103 or the anti-TNFα / anti-OX40L bispecific ISVD constructs F027300252, F027301104, F027301189, F027301197, and F027301199, incubation with a combination of recombinant human TNFα and recombinant human OX40L (hTNFα+OX40L) was used (Figures 6 and 7). HH or anti-OX40L V HHAlthough treatment with either construct alone resulted in some inhibition of luciferase activity induced by the hTNFα+OX40L combination, up to approximately <70%, only treatment with the bispecific anti-TNFα / anti-OX40L ISVD construct was able to completely suppress the induction of luciferase activity to 100% (Figure 6). Furthermore, calculation of IC50 values ​​for the anti-TNFα / anti-OX40L bispecific ISVD constructs F027300252, F027301104, F027301189, F027301197, and F027301199 at various concentrations ranging from 1 pM to 20 nM indicated that ISVD constructs F027300252 and F027301199 exhibited the most potent efficacy in this particular set of experiments (Figure 7).

[0244] [Table 19]

[0245] 6.11 Example 11: Inhibition of OX40L and TNFα by multispecific ISVD constructs reduces GM-CSF levels in a mixed lymphocyte reaction (MLR) To test the physiological effects of OX40L blockade on T cell activation, a mixed lymphocyte reaction assay was performed. Briefly, monocyte-derived dendritic cells (MoDCs) from healthy blood donors were matured in vitro to express OX40L, and then these cells were mixed with PBMCs from another unrelated healthy donor. Mixing the unrelated donors in the same well induced alloreactivity and T cell activation. The allogeneic T cell response was monitored by measuring cytokines in the supernatant 5 days after cell mixing. Detailed descriptions of MoDC preparation and evaluation of T cell responses by cytokine measurement are provided below.

[0246] Preparation of monocyte-derived dendritic cells from PBMCs of healthy blood donors: PBMCs were isolated from whole blood or buffy coats by gradient centrifugation. Cells were counted and plated at 3 × 10 per well in 3 ml RPMI 1640 medium containing Glutamax, 10% human serum, 10 mM Hepes, and 20 μg / ml gentamicin in a 6-well plate. 7 After 1-2 hours, non-adherent cells were washed with three rounds of washing, and the cells were incubated for 5 days in the presence of 500 IU / ml IL-4 and 500 IU / ml GM-CSF. On the third day of this 5-day incubation, the medium was partially replaced with fresh medium containing IL-4 and GM-CSF. At the end of the 5-day incubation period, differentiated but still immature DCs were collected and counted. DCs were then replated (5 x 10) for further maturation in 6- or 24-well plates. 5 DCs were incubated in the same medium (as above) containing a novel cytokine cocktail [500 IU / ml IL-4, 500 IU / ml GM-CSF, 10 ng / ml IL-1b, 1000 IU IL-6, 10 ng / ml TNFα, and 1 μg / ml PGE2], which was identified as the most suitable cytokine for inducing OX40L expression in DCs among other stimuli (Figure 8). On days 2, 3, and 4 of maturation, DCs were collected and the expression of maturation markers, including CD86, CD83, CD40, HLA-DR, and OX40L, was assessed by flow cytometry. After 3–4 days of maturation, 30–70% of all mature DCs expressed OX40L on their surface. After confirming OX40L expression (Figure 8), DCs were frozen in freezing medium (90% fetal bovine serum + 10% DMSO) for later use in MLR assays.

[0247] mixed lymphocyte reaction PBMCs were isolated from whole blood or buffy coats by gradient centrifugation. Cells were resuspended in X-Vivo15 medium (Lonza) and counted. Meanwhile, DCs were thawed and resuspended in X-Vivo15 medium. 1 × 10 5 5 x 10 PBMCs and 5 x 10 3DCs were mixed in the same well of a U-bottom 96-well plate. To characterize the effects of treatment with anti-TNFα (10 μg / ml) alone, anti-OX40L (10 μg / ml), or the combination of anti-TNFα (10 μg / ml) and anti-OX40L (10 μg / ml), the antibodies were added at the appropriate dilutions, and the mixture of PBMCs and DCs was incubated for 5 days. Similarly, to characterize the effects of treatment with the ISVD construct, various concentrations of F027300252 (400–0.13 nM; 5-fold serial dilutions) or control V were added. HH IRR00119 was added, and the mixture of PBMCs and DCs was incubated for 5 days. Incubation with a control isotype IgG (Biolegend; clone QA16A12) was used as a negative control. After 5 days, the supernatants were collected, and the amounts of various cytokines in the supernatants were evaluated using a Luminex-based multiplex assay (Figure 9). To determine anti-TNFα and / or anti-OX40L efficacy, DCs from three different human donors were tested against PBMCs from five allogeneic donors. To determine the IC50 value for inhibiting GM-CSF production by F027300252, PBMCs from eight donors were tested against DCs from two allogeneic donors. Speed's XLfit program was used to fit the dose-response curve and calculate the IC50 value for F027300252.

[0248] Treatment with anti-TNFα or anti-OX40L antibodies alone, both at saturating concentrations of 10 μg / ml, led to a significant inhibition of GM-CSF secretion compared to treatment with the isotype (FIG. 9). However, treatment with a combination of anti-TNFα and anti-OX40L antibodies did not significantly inhibit the individual TNF blockade ( ** p<0.0016; Figure 9 ) or OX40L blockade ( ****p<0.0001; Figure 9). This suggests the potency of combined blockade of TNFα and OX40L is superior (Figure 9). To determine the IC50 value for inhibition of GM-CSF production by F027300252, PBMCs from eight donors were tested against DCs from two allogeneic donors. Speed's XLfit program was used to fit the dose-response curve and calculate IC50 values. F027300252 inhibited GM-CSF production in a concentration-dependent manner with an IC50 of 51.69 ± 19.3 nM (SEM) and a maximum inhibition of 70.32 ± 5.59%.

[0249] 6.12 Example 12: Multispecific ISVD Construct Binding to Existing Antibodies Binding of pre-existing antibodies present in 96 serum samples from healthy volunteers to ISVD construct F027300252 was determined using a ProteOn XPR36 (Bio-Rad Laboratories, Inc.) PBS / Tween (phosphate-buffered saline, pH 7.4, 0.005% Tween 20) was used as the running buffer, and experiments were performed at 25°C.

[0250] ISVD constructs were captured on the chip via binding of the ALB building blocks to HSA immobilized on the chip. For HSA immobilization, the ligand lane of a ProteOn GLC sensor chip was activated with EDC / NHS (flow rate 30 μl / min), and 100 μl / ml of HSA in ProteOn acetate buffer pH 4.5 was injected to achieve an immobilization level of approximately 3200 RU. After immobilization, the surface was inactivated with ethanolamine HCl (flow rate 30 μl / min).

[0251] The ISVD construct was then injected over the HSA surface at 45 μl / min for 2 minutes, resulting in an ISVD construct capture level of approximately 800 RU. Samples containing pre-existing antibodies were centrifuged at 14,000 rpm for 2 minutes, and the supernatant was diluted 1:10 with PBS-Tween 20 (0.005%) and then injected at 45 μl / min for 2 minutes, followed by a 400-second dissociation step. After each cycle (i.e., before the new ISVD construct capture and blood sample injection step), the HSA surface was regenerated by injecting HCl (100 mM) at 45 μl / min for 2 minutes. Sensorgrams showing pre-existing antibody binding after double referencing were obtained by subtracting 1) ISVD-HSA dissociation and 2) nonspecific binding to the reference ligand lane. The level of pre-existing antibody binding was determined by setting the reporting time point to 125 seconds (5 seconds after the end of binding). The percentage reduction in pre-existing antibody binding was calculated relative to the binding level of the reference ISVD construct at 125 seconds.

[0252] The pentavalent ISVD construct F027300252, which was optimized to reduce pre-existing antibody binding by introducing the mutations L11V and V89L and a C-terminal alanine into each building block, showed significantly reduced binding to pre-existing antibodies compared to the control non-optimized pentavalent ISVD construct F027301186 (Table 6, Table 14, Figure 10 and Figure 11).

[0253] Pre-existing antibody binding depends on the valency and composition of the multispecific construct. Table 6 and Figure 10 demonstrate that the pentavalent ISVB construct F027300252 exhibited lower pre-existing antibody reactivity than the tetravalent ISVD constructs F027301104 and F027301099.

[0254] Four ISVD constructs composed of the same parent building blocks as ISVD construct F027300252 exhibited different pre-existing antibody reactivity (Table 14, Figure 11). Comparing ISVD construct F027300028 to ISVD construct F027301186 shows that the introduction of the L11V and V89L mutations and the C-terminal alanine in each building block significantly reduced pre-existing antibody reactivity. Comparing ISVD construct F027300028 to ISVD construct F027301097 shows that the introduction of the T110K mutation in the C-terminal building block slightly further reduced pre-existing antibody reactivity. Replacing the 35GS linker in ISVD construct F027301097 with the shorter 9GS linker in ISVD construct F027300252 further significantly reduced pre-existing antibody reactivity. Therefore, it was important to use short 9GS linkers in multivalent constructs to obtain low pre-existing antibody reactivity.

[0255] [Table 20]

[0256] 6.13 Example 13: Evaluation of the multispecific anti-TNFα / OX40L ISVD construct F027300252 in the chronic human TNFα transgenic Tg197 polyarthritis model. The F027300252 polyspecific anti-TNFα / OX40L ISVD construct was profiled in the Tg197 mouse model of TNF-driven progressive polyarthritis (Keffer et al., 1991, EMBO J. 10:4025-4031). In these mice, a modified human TNFα gene was inserted as a transgene into the mice. The human gene was modified to make the transcribed mRNA more stable, leading to overexpression of TNFα and spontaneous, progressive arthritis in all four limbs with 100% penetrance. Signs and symptoms became evident at approximately 6 weeks of age and steadily increased until, if left untreated, significant moribundity and death occurred after approximately 10 weeks of age. Arthritis severity was assessed clinically using a scoring system as detailed below.

[0257] [Table 21]

[0258] The arthritis was responsive to treatment with therapeutic agents directed against the inhibition of human TNFα (Shealy et al., 2002, Arthritis Res. 4(5):R7).

[0259] To establish dose-dependent efficacy, 6-week-old animals with clear signs and symptoms of arthritis were administered different doses of the ISVD construct in a therapeutic manner via intraperitoneal injection twice weekly (n = 8 animals per group). Human IgG1 purified from human myeloma serum (BioXcell #BE0297) was used as a negative control, and an anti-hTNFα reference mAb was used as a positive control for arthritis suppression. The F027300252 ISVD construct was administered at four different dose intensities: 1 mg / kg body weight, 3 mg / kg, 10 mg / kg, and 30 mg / kg, respectively. Treatment continued until 11 weeks of age. Clinical arthritis scores were determined weekly. As shown in Figure 12, ISVD construct treatment resulted in a dose-dependent suppression of clinical arthritis scores over time.

[0260] Animals treated with the human IgG1 negative control antibody developed a mean arthritis score of 1.58 ± 0.06 by week 11. The anti-hTNFα reference mAb completely inhibited arthritis progression by week 11, with a mean score of 0.61 ± 0.06. F027300252 reduced arthritis progression by week 11, with mean scores of 1.30 ± 0.09 (1 mg / kg), 0.89 ± 0.10 (3 mg / kg), 0.67 ± 0.08 (10 mg / kg), and 0.28 ± 0.04 (30 mg / kg). Overall arthritis inhibition was analyzed by area under the curve (AUC, Figure 13). All doses of F027300252 significantly inhibited arthritis progression in the Tg197 arthritis model, comparable to the anti-hTNFα reference mAb.

[0261] At the completion of treatment, hind ankle joints were processed for histology and sections were evaluated for structural signs of arthritis using the following scoring system:

[0262] [Table 22]

[0263] The results of histological scoring are shown in Figure 14. F027300252 significantly inhibited structural arthritis and joint destruction at higher doses.

[0264] In conclusion, these results demonstrate a dose-dependent suppression of arthritis signs and symptoms and inhibition of structural progression by ISVD construct F027300252 to a similar extent as the anti-hTNFα reference mAb.

[0265] 6.14 Example 14: Evaluation of anti-TNF / OX40L ISVD constructs in a human TNFα-driven acute rheumatoid arthritis mouse model (CAIA). The in vivo efficacy of the anti-TNF-OX40L ISVD construct (F027300252) was evaluated in an acute rheumatoid arthritis model called collagen antibody-induced arthritis (CAIA). CAIA is a preclinical model of rheumatoid arthritis and is widely used to evaluate anti-arthritic drug efficacy in drug development (Nandakumar & Holmdahl (2007), Methods Mol Med.; 136:215-23). ​​This is a short-term (7-day) induced arthritis model using a cocktail of monoclonal anti-collagen II antibodies and LPS. For this experiment, humanized TNFα and TNFR1 mice: C57BL / 6NTac-Tnfrsf1a tm4504.1(TNFRSF1A)Tac TNF tm4503.1(TNF)TacAll animals were medicated and monitored in accordance with the guidelines of the Institutional Animal Care and Use Committee for research protocols approved by the Sanofi Laboratory Animal Welfare Committee under permission from the German animal welfare government agency. In vivo arthritis scores were assessed in an operator-blinded manner. Male and female mice, at least 10 weeks old, were equally randomized to each treatment group. Mice received 8 mg of a cocktail of monoclonal anti-collagen antibodies (ArthritoMab, MDbiosciense, CIA-MAB-2C) by intraperitoneal (ip) injection in sterile PBS on day 0, followed by 25 μg of LPS in PBS 24 hours later. Mice were monitored for 7 days. Treatment consisted of 6 hours after LPS on day 1 with an isotype control (IgG1 isotype, 1.0 mg / kg i.p., 200 μl / mouse) or the multispecific TNFα-OX40L ISVD construct F027300252 at 0.03, 0.1, 0.3, or 1 mg / kg (200 μl / mouse) compared to an anti-hTNFα reference mAb (conventional antibody) at 0.1 and 0.5 mg / kg in 200 μl / mouse. The applied doses were equivalent to estimated molar exposures of 0.45, 1.5, 4.5, and 15 nmol / kg for F027300252 and 0.65 and 3.3 nmol / kg for the anti-hTNFα reference mAb. For the anti-hTNFα reference mAb, two studies were performed, and vehicle animals were pooled for final analysis. A second dose was administered to all animals three days after the first dose on day 4 of the experiment. The schematic study design of the experiment is illustrated in FIG.

[0266] The results of the experiment are shown in Figure 16. Vehicle-treated control animals from two experiments with different anti-hTNFα reference mAb concentrations tested against the control were pooled. A mean peak increase in arthritis score of 6.135 was achieved on day 6. The positive control anti-hTNFα reference mAb showed a significant effect on arthritis score, and the higher concentration tested (3.3 nmol / kg) completely blocked disease development. The anti-TNFα-OX40L ISVD construct F027300252 showed a dose-dependent effect with similar in vivo efficacy compared to the anti-hTNFα reference mAb. Only the lowest dose of 0.45 nmol / kg showed approximately 50% efficacy, equivalent to the lower dose of anti-hTNFα reference mAb at 0.65 nmol / kg, while all other doses tested for the ISVD construct completely blocked disease development. The second lowest dose tested for F027300252, 1.5 nmol / kg, appears to be at least as effective if not more effective than the 3.3 nmol / kg anti-hTNFα reference mAb.

[0267] The significant dose-dependent effect of the ISVD construct F027300252 is more evident in Figure 17, where the AUC of the arthritis score data from Figure 16 is plotted. All doses of F027300252 significantly reduced arthritis scores, from 1.5 nmol / kg to more Higher doses of hTNFα resulted in complete disease inhibition, and efficacy was comparable to that of an anti-hTNFα reference mAb at equimolar exposure / dose.

[0268] In conclusion, these results demonstrate that the anti-TNF-OX40L ISVD construct was as good or potentially superior in targeting human TNFα compared to an anti-hTNFα reference mAb in a murine acute rheumatoid arthritis model, thereby highlighting its immunosuppressive potential in the treatment of autoimmune diseases such as rheumatoid arthritis. Statistics: One-way ANOVA and Bonferroni multiple comparison test.

[0269] 6.15 Example 15: Mechanism demonstration in a combined non-human primate T cell dependent antibody response (TDAR) and delayed type hypersensitivity (DTH) model with F027300252. The T cell-dependent antibody response (TDAR) model is a measure of immune function that depends on the effectiveness of multiple immune processes, including antigen uptake and presentation, T cell help, B cell activation, and antibody production. In this study, we used this model to determine the pharmacodynamic effects of the anti-TNF-OX40L ISVD construct F027300252 in vivo in non-human primates. The objective of this study was to determine the pharmacodynamics, as well as the PK and safety of F027300252 administered subcutaneously five times weekly to female cynomolgus monkeys, followed by a 30-day treatment-free period after the final dose of F027300252 on day 29. To assess the effect of F027300252 on immune system functionality, humoral responses were assessed during life by TDAR assay (post-keyhole limpet hemocyanin-KLH-immunization), and cellular immune responses were assessed by in vivo delayed-type hypersensitivity (DTH) tests and ex vivo ELISPOT assays using the same antigens as for TDAR and KLH.

[0270] A total of 20 female laboratory cynomolgus monkeys (Macaca fascicularis) were used in this study. Because F027300252 binds highly selectively to its human target, we selected nonhuman primates as the species. Nonhuman primates were selected based on the high cross-reactivity of F027300252 to cynomolgus monkeys. The test item is not cross-reactive with other rodent or non-rodent species. Therefore, based on available data, cynomolgus monkeys were selected as the non-rodent species for pharmacology and nonclinical safety studies. Background data from previous studies are available at Citoxlab France, the contract research organization (CRO) where we work. Additionally, the TDAR and DTH assays have previously been validated in cynomolgus monkeys at this CRO.

[0271] The preliminary safety of F027300252 was evaluated in a repeat-dose cynomolgus monkey study of 25 mg / kg administered by the subcutaneous (sc) route, administered twice, two weeks apart (Study No. DIV1953). The doses selected for this TDAR-DTH combination study spanned a range encompassing both potential pharmacological doses (3 and 10 mg / kg) and higher doses (30 and 100 mg / kg) for further safety evaluation. The dose formulation was administered once weekly for 29 days, for a total of five doses. Day 1 corresponded to the first day of the treatment period with the test and control items.

[0272] The pharmacokinetics of selected doses are illustrated in FIG.

[0273] KLH antigen was administered subcutaneously at a dose of 10 mg / animal in 1 ml on days 3 and 31 (Figure 18). We used Imject® Marine-Cultivated Keyhole Limpet Hemocyanin (ThermoFisher Scientific, Reference Number 77600). For quantification of anti-KLH IgG, venous blood (1 mL) was collected from the appropriate vein into plain tubes according to a predetermined schedule. The blood was kept at room temperature until clotting (maximum 2 hours), centrifuged (approximately 3000 g, +4°C for 10 minutes), and the resulting serum was divided into three 80 μL aliquots plus one aliquot of the remaining volume. The tubes were stored frozen at -20°C until analysis. Anti-KLH IgG levels were measured using a specific ELISA method developed and validated by Citoxlab France (Citoxlab France / Study Number 41106RD for anti-KLH IgG). The area under the curve (AUC) was calculated for each animal for each of two time periods (day 3 [before KLH injection] to day 31 [before KLH injection] and day 31 [before KLH injection] to day 59). AUC values ​​were transformed to logarithm (log10(value + 1)) and analyzed by time period using the Wilcoxon test.

[0274] As expected, the primary IgG response to KLH was minor. After a second exposure to KLH, a strong anti-KLH IgG response was induced in the vehicle control-treated group. Treatment with F027300252 resulted in a strong inhibition of this response from the lowest dose tested to higher doses (Figure 20). The AUC of the F027300252 study was plotted compared with data from a preliminary study using monoclonal antibody tools against TNFα (anti-hTNFα reference mAb) and OX40L (anti-hOX40L reference mAb) (Figure 21). Even at the lowest dose tested (3 mg / kg) of F027300252 (referred to as "nanobody" in Figure 21), we observed a more pronounced reduction in AUC compared with anti-hOX40L reference mAb and anti-hTNFα reference mAb monotherapy.

[0275] At necropsy, we collected peripheral blood mononuclear cells (PBMCs) from the monkeys, restimulated them ex vivo with the same antigen (KLH), and measured the cellular immune response using an enzyme-linked immunospot (ELISPOT) assay. The ELISPOT assay is a highly sensitive immunoassay that measures the frequency of cytokine-secreting cells at the single-cell level. In this assay, cells were seeded into wells of a 96-well plate precoated with capture antibodies specific for the cytokine being assayed (in this case, IFN-γ and IL-4). Cytokines secreted by the cells in the presence (or absence) of stimulation were captured by the specific antibodies on the surface of the well bottom near the secreting cells. After an appropriate incubation period, the cells were removed, and secreted cytokines were visualized using a biotinylated detection antibody. After several washing steps, an enzyme (alkaline phosphatase) coupled to streptavidin was added. The immobilized cytokines were then revealed as ImmunoSpots (i.e., individual cytokine-secreting cells) using a precipitation substrate. Each PBMC sample was analyzed for the frequency of IFN-γ and IL-4 secreting cells after stimulation with KLH.

[0276] The ELISPOT plates were numerically scanned in the laboratory using an Immunospot® ELISPOT analyzer (images of individual wells were taken with this instrument). The images were then analyzed with dedicated software for spot count evaluation. The presence and number of spots in each well were assessed, and the corresponding results, expressed as the number of IFN-γ or IL-4 spot-forming cells (sfc), were calculated. The number of sfc was normalized per million PBMCs.

[0277] Stimulator cells from vehicle control animals showed a significant increase in both IFN-γ (Figure 22) and IL-4 (Figure 23). Upon treatment, both cytokines were significantly reduced at all doses tested for F027300252 (Figures 22 and 23).

[0278] In conclusion, these results demonstrate that the anti-TNF-OX40L ISVD construct potently inhibited interactions between antigen-presenting cells, T cells, and B cells in a mechanistic model of T cell-dependent antibody responses performed in non-human primates.

[0279] The second part of the non-human primate study focused on in vivo delayed-type hypersensitivity (DTH) readouts in the skin to assess cell-mediated immune responses during life. Tetanus toxoid (TTx) and aluminum hydroxide were used as antigens. DTH challenge was applied as described in Figure 18. A grid approximately 21 cm long by 3 cm wide (i.e., 3 cm squares) was delineated with an indelible surgical pen on the skin on each side of the back (Figure 24). The dot in the center of each square indicates the injection site. The injection site was disinfected with chlorhexidine gluconate solution (Antisept® spray).

[0280] The antigens (TTx / ALU and KLH) were injected into the center of each of the six squares on the day of injection. Intradermal injections were performed using a sterile, single-use plastic syringe fitted with a sterile, single-use 29G needle by stretching the skin and introducing the needle into the thickness of the skin (at an oblique angle). A small bleb appeared at the injection site. The needle was then quickly withdrawn from the skin.

[0281] During the in-life phase of DTH, the following parameters were assessed and documented (Table 15).

[0282] [Table 23]

[0283] None of the parameters listed above showed a clear trend towards a treatment effect with F027300252, indicating that histopathological evaluation and immunohistochemistry may demonstrate differences more sensitively than gross skin changes during the in-life phase of the DTH portion of this model.

[0284] Immunohistochemistry evaluated the following markers: CD3 (T lymphocytes), CD4 (T helper cells), CD8 (cytotoxic T cells), CD30 (B lymphocytes), CD68 (macrophages), Ki67 (proliferation marker), and FoxP3 (T regulatory cells). The results of conventional H&E histopathology and immunohistochemistry (IHC) staining are summarized below.

[0285] For the first antigen, TTx / ALU, the immune response was dominated by macrophages at day 34. A slight dose-dependent decrease in inflammation severity was observed, with the most pronounced effect observed at the highest dose. Immunohistochemistry revealed a significant decrease in all marker scores, primarily in macrophages, at the highest dose tested. At necropsy on day 59, a slightly lower overall inflammatory response was observed compared to day 34. As at day 34, IHC revealed the most pronounced effect on the proliferation marker Ki67. Overall, the effect was most pronounced at the highest dose tested for F027300252.

[0286] For the second antigen tested, KLH, observations on day 34 showed an immune response dominated by eosinophilic granulocytes. Overall, the inflammatory response was lower and more moderate than that of TTX / Alu on day 34. IHC revealed a significant decrease in all marker scores at the highest dose tested, primarily in CD8+, CD20, and Ki67. At day 59, the inflammatory response was again lower and more moderate than that of TTX / Alu on day 59 and lower and more minimal than that of KLH on day 34. This was the smallest decrease observed, with a clear dose-response relationship in the severity of inflammation. As with day 34, IHC revealed a predominant decrease in CD3, CD8+, CD20, and FoxP3, for which the effect was most pronounced at the highest F027300252 dose tested.

[0287] Overall, the described data provide mechanistic proof of concept in a combined non-human primate T cell-dependent antibody response (TDAR) and delayed-type hypersensitivity (DTH) model with F027300252, a novel multispecific ISVD construct targeting TNFα and OX40L, confirmed by KLH-induced ex vivo ELISPOT assays measuring IFN-γ and IL-4 release.

[0288] 6.16 Example 16: Evaluation of anti-TNF / OX40L ISVD construct F027300252 in a xenograft-versus-host disease model. The in vivo efficacy of the anti-TNFα / OX40L ISVD construct F027300252 was evaluated in a model of xenograft-versus-host disease (xeno-GVHD) in which human peripheral blood mononuclear cells (hPBMCs) were injected into irradiated, immunodeficient NOD-scid IL2rgamma(null) (NSG) mice (King et al., (2009), Clin Exp Immunol., 157(1):104-118). The transplanted hPBMCs attack the mouse host in a major histocompatibility complex-dependent manner, leading to the development of acute GVHD symptoms (Brehm et al., (2019), FASEB J., 33(3):3137-3151).

[0289] Female NSG mice, at least 6 years old, were randomized equally to each treatment group. 7 Mice were irradiated with 1 G γ one day before intravenous (iv) injection of hPBMCs. Animals were scored individually, operator-blinded, three times weekly using the following scoring system (Riesner et al., (2016) Bone Marrow Transplant. 51(3):410-417):

[0290] [Table 24]

[0291] A GVHD score was determined by summing these parameters. Animals were euthanized if a single score of 2 was reached or if the cumulative score exceeded 6. The extent of hPBMC engraftment in host mice was assessed by determining human CD45+ cells among total CD45+ cells in the peripheral blood of host mice using flow cytometry. Bispecific anti-TNF / OX40L Nanobody F027300252 (10 mg / kg) was administered IP three times weekly starting on day 1 and compared with isotype-treated control animals. All animals were medicated and monitored in accordance with the Institutional Animal Care and Use Committee guidelines for research protocols approved by the Sanofi Laboratory Animal Welfare Committee under permission from the German Agency for Animal Welfare.

[0292] To validate TNF and OX40L as targets of ISVD constructs in a xenogeneic GVHD mouse model, 150 nmol / kg anti-human TNF ISVD F027500018, 150 nmol / kg anti-human OX40L ISVD F027300044, or bispecific anti-TNF / OX40L ISVD F027300252 (150 nmol / kg) was administered IP three times a week starting on day 1 and compared with isotype-treated control animals. The first symptoms of GVHD in host mice were observed within two weeks after hPBMC injection. In isotype-treated control animals, GVHD scores continuously increased as the study progressed until all mice in this group were found to have either died or reached the humane endpoint described above and were euthanized. While TNF blockade had only a mild effect on disease development, OX40L blockade was able to significantly ameliorate disease development (Figure 25). Dual targeting with F027300252 treatment resulted in disease onset similar to that observed with OX40L blockade alone. Consequently, survival was slightly extended in the F027500018-treated group, whereas anti-TNF / OX40L combination treatment with F027300044 alone or F027300252 resulted in further survival extension (Figure 26). In addition, OX40L blockade using either F02730044 or F027300252 tended to inhibit engraftment of human CD45+ cells in host mice (Figure 27).

[0293] To evaluate the bispecific anti-TNF / OX40L ISVD F027300252 in a xenogeneic GVHD mouse model, additional data were collected and the results of two independent studies pooled. GVHD onset was observed within a few days in hPBMC-transplanted mice. It was found that 50% of animals receiving the isotype ISVD alone died or reached discontinuation criteria within 5 weeks after transplantation. While F027300252 treatment did not prevent disease onset, it was able to ameliorate disease progression (Figure 28), thereby extending the survival of F027300252-treated mice. More than 50% of the animals remained alive beyond week 9 (Figure 29). In some cases, mice treated with F027300252 recovered from disease and survived until the end of the study. Furthermore, application of F027300252 was found to inhibit the engraftment of human CD45+ cells in host NSG mice (Figure 30). This correlates with delayed disease onset and prolonged survival.

[0294] Collectively, these results demonstrate the efficacy of OX40L blockade and F027300252 treatment in a xenogeneic GVHD mouse model.

[0295] 6.17 Example 17: Inhibition of PHA-induced IL-8 release in human whole blood by anti-TNF antibodies and bispecific anti-TNFα / anti-OX40L ISVD constructs F027300252, F027301104, F027301189, F027301197, and F027301199. To measure the effects of anti-TNFα monoclonal antibody (mAb) RA14956298 (from Sanofi) and bispecific anti-TNFα / anti-OX40L ISVDs F027300252, F027301104, F027301189, F027301197, and F027301199 (all from Sanofi) on inhibiting PHA-induced IL-8 release in human whole blood, blood from healthy human donors was collected in vacutainer tubes (BD#368480) in the presence of Na-heparin [17 IU / ml] as an anticoagulant. PHA-L (phytohemagglutinin-L; from Merck Millipore; order number #M5030) was reconstituted in sterile water as a stock solution [1 mg / ml] to prepare a working solution containing [50 μg / ml] PHA-L. Negative control antibody RA11944493 (Sanofi; IgG1 isotype control), positive control antibody RA14956298 (from Sanofi; negative control V HH Working solutions of IRR00119 (from Sanofi / Ablynx) and bispecific anti-TNFα / anti-OX40L ISVD constructs F027300252, F027301104, F027301189, F027301197, and F027301199 (all from Sanofi / Ablynx) were prepared to 500 nM in PBS.

[0296] Serial dilutions of antibodies and ISVD constructs (25 μL each) at final concentrations of 8 pM to 25 nM in medium [RPMI-1640 (Gibco; order number 61870-010) + 10% human AB serum (Sigma; order number H3667) + 1% PenStrep (Gibco; order number 15140-122)] were added to a 96-well microplate (V-bottom, PP; Eppendorf; order number 0030601300). 200 μL of human blood was added to each well, the plate was covered, and the plate was incubated at room temperature for 30 min. PHA-L was diluted to a concentration of 50 μg / ml in medium [RPMI-1640 + 10% human AB serum + 1% PenStrep], and 25 μL of this PHA-L in medium was added to each well of the 96-well plate containing the preincubation mixture of human blood and antibody or ISVD construct. The samples were gently mixed, the plates were sealed with sterile lids (using Thermo Scientific plate sealers, order number 236366), and the plates were incubated at 37°C, 5% CO2, and 95% rH2O for 6 hours. After incubation, the blood samples were centrifuged at 2000 x g for 12 minutes, using an intermediate gradient for acceleration and disruption. Plasma supernatants were collected and stored at -80°C in new 96-well microplates for further analysis by ELISA. IL-8 levels were determined using an enzyme-linked immunosorbent assay (ELISA; from Invitrogen; catalog number 88-8086) to quantitatively detect human IL-8 according to the manufacturer's protocol. Based on the results of three blood donors, the XLfit program in Speed ​​was used to fit the dose-response curves shown in Figure 31 and calculate IC50 values.

[0297] Negative control antibody RA11944493 or negative control V HHIncubation of human whole blood with IRR00119 did not result in any inhibition of PHA-induced IL-8 release (data not shown). In contrast, incubation of human whole blood with the monospecific anti-TNF monoclonal antibody (mAB) RA14956298 resulted in potent inhibition of PHA-induced IL-8 release with an IC50 of 0.96 nM (±0.08 SEM) (Figure 31). Incubation of human whole blood with the bispecific anti-TNFα / anti-OX40L ISVD construct F027300252 resulted in even more potent inhibition of PHA-induced IL-8 release with an IC50 of 0.33 nM (±0.09 SEM) (Figure 31). The bispecific anti-TNFα / anti-OX40L ISVD constructs F027301104, F027301197, and F027301199 also led to potent inhibition of PHA-induced IL-8 release with IC50 values ​​of 0.8233 (±0.4 SEM), 0.5667 (±0.27 SEM), and 0.3133 (±0.08 SEM), respectively (Figure 31). The bispecific anti-TNFα / anti-OX40L ISVD construct F027301189 showed the lowest potency with an IC50 of 2.143 (±1.54 SEM) (Figure 31). [Industrial Applicability]

[0298] 7 Industrial Applicability The polypeptides, nucleic acid molecules encoding them, vectors comprising the nucleic acids, and compositions described herein can be used, for example, to treat subjects suffering from autoimmune or inflammatory diseases.

Claims

1. A polypeptide, a composition comprising said polypeptide, or a composition comprising a nucleic acid comprising a nucleotide sequence encoding said polypeptide, wherein said polypeptide comprises or consists of at least four immunoglobulin single variable domains (ISVDs), each of said ISVDs comprising three complementarity determining regions (CDR1 to CDR3, respectively), optionally linked via one or more peptide linkers; a. the first ISVD and the second ISVD each specifically bind to OX40L, and each i. a CDR1 comprising the amino acid sequence of SEQ ID NO: 7 or having two or one amino acid difference from SEQ ID NO: 7; ii. CDR2 comprising the amino acid sequence of SEQ ID NO: 10 or having two or one amino acid difference from SEQ ID NO: 10; and iii. CDR3 comprising the amino acid sequence of SEQ ID NO: 13 or having two or one amino acid difference from SEQ ID NO: 13 Including; b. the third ISVD and the fourth ISVD each specifically bind to TNF-α, each comprising: iv. CDR1 comprising the amino acid sequence of SEQ ID NO: 8 or having two or one amino acid difference from SEQ ID NO: 8; v. CDR2 comprising the amino acid sequence of SEQ ID NO: 11 or having two or one amino acid difference from SEQ ID NO: 11; and vi. CDR3 comprising the amino acid sequence of SEQ ID NO: 14 or having two or one amino acid difference from SEQ ID NO: 14 a composition comprising the peptide, the polypeptide, or a nucleic acid comprising a nucleotide sequence encoding the polypeptide,

2. a. the first ISVD and the second ISVD each comprise a CDR1 comprising the amino acid sequence of SEQ ID NO:7, a CDR2 comprising the amino acid sequence of SEQ ID NO:10, and a CDR3 comprising the amino acid sequence of SEQ ID NO:13; b. The polypeptide or composition of claim 1, wherein the third ISVD and the fourth ISVD each comprise a CDR1 comprising the amino acid sequence of SEQ ID NO:8, a CDR2 comprising the amino acid sequence of SEQ ID NO:11, and a CDR3 comprising the amino acid sequence of SEQ ID NO:

14.

3. a. the amino acid sequence of the first ISVD comprises greater than 90% sequence identity with SEQ ID NO:2; b. the amino acid sequence of the second ISVD comprises greater than 90% sequence identity with SEQ ID NO:3; c. the amino acid sequence of the third ISVD comprises greater than 90% sequence identity with SEQ ID NO:4; d. The polypeptide or composition of claim 1 or 2, wherein the amino acid sequence of the fourth ISVD comprises greater than 90% sequence identity with SEQ ID NO:

6.

4. a. the first ISVD comprises the amino acid sequence of SEQ ID NO:2; b. the second ISVD comprises the amino acid sequence of SEQ ID NO:3; c. the third ISVD comprises the amino acid sequence of SEQ ID NO:4; d. The polypeptide or composition of any one of claims 1 to 3, wherein the fourth ISVD comprises the amino acid sequence of SEQ ID NO:

6.

5. 5. The polypeptide or composition of claim 1, wherein the polypeptide further comprises one or more other groups, residues, moieties or binding units, optionally linked via one or more peptide linkers, which provide the polypeptide with an increased half-life compared to a corresponding polypeptide that does not have said one or more other groups, residues, moieties or binding units.

6. 6. The polypeptide or composition of claim 4 or 5, wherein the one or more other groups, residues, moieties, or binding units that provide the polypeptide with an increased half-life are selected from the group consisting of binding units capable of binding to serum albumin (such as human serum albumin) or serum immunoglobulin (such as IgG).

7. 7. The polypeptide or composition of claim 6, wherein the binding unit that provides the polypeptide with increased half-life is an ISVD capable of binding to human serum albumin.

8. The ISVD that binds to human serum albumin is i. a CDR1 comprising the amino acid sequence of SEQ ID NO: 9 or having two or one amino acid difference from SEQ ID NO: 9; ii. CDR2 comprising the amino acid sequence of SEQ ID NO: 12 or having two or one amino acid difference from SEQ ID NO: 12; and iii. CDR3 comprising the amino acid sequence of SEQ ID NO: 15 or having two or one amino acid difference from SEQ ID NO: 15 8. The polypeptide or composition of claim 7, comprising:

9. The polypeptide or composition of claim 7 or 8, wherein the ISVD that binds to human serum albumin comprises a CDR1 comprising the amino acid sequence of SEQ ID NO: 9, a CDR2 comprising the amino acid sequence of SEQ ID NO: 12, and a CDR3 comprising the amino acid sequence of SEQ ID NO:

15.

10. The polypeptide or composition of any one of claims 7 to 9, wherein the amino acid sequence of the ISVD that binds to human serum albumin comprises more than 90% sequence identity with SEQ ID NO:

5.

11. The polypeptide or composition of any one of claims 7 to 10, wherein the ISVD that binds to human serum albumin comprises the amino acid sequence of SEQ ID NO:

5.

12. The polypeptide or composition of any one of claims 1 to 11, wherein the amino acid sequence of the polypeptide comprises more than 90% sequence identity with SEQ ID NO:

1.

13. The polypeptide according to any one of claims 1 to 12, wherein the polypeptide comprises or consists of the amino acid sequence of SEQ ID NO:

1.

14. A nucleic acid comprising a nucleotide sequence encoding a polypeptide according to any one of claims 1 to 13.

15. A host or host cell comprising the nucleic acid of claim 14.

16. A method for producing a polypeptide according to any one of claims 1 to 13, comprising at least a. expressing the nucleic acid of claim 14 in a suitable host cell or host organism or in another suitable expression system; optionally followed by: b) isolating and / or purifying the polypeptide according to any one of claims 1 to 13. The method comprising:

17. A composition comprising at least one polypeptide according to any one of claims 1 to 13 or a nucleic acid according to claim 14.

18. 18. The composition of claim 17, which is a pharmaceutical composition further comprising at least one pharmaceutically acceptable carrier, diluent or excipient, and / or adjuvant, and optionally one or more additional pharmaceutically active polypeptides and / or compounds.

19. A polypeptide according to any one of claims 1 to 13 or a composition according to claim 17 or 18 for use as a medicament.

20. A polypeptide according to any one of claims 1 to 13 or a composition according to claim 17 or 18 for use in the treatment of an autoimmune or inflammatory disease.

21. 21. The polypeptide or composition for use according to claim 20, wherein the autoimmune or inflammatory disease is selected from rheumatoid arthritis, inflammatory bowel diseases such as Crohn's disease and ulcerative colitis, psoriasis, hidradenitis suppurativa, and graft-versus-host disease.

22. 19. A method for treating an autoimmune or inflammatory disease, comprising administering to a subject in need thereof a pharmaceutically active amount of a polypeptide according to any one of claims 1 to 13 or a composition according to claim 17 or 18.

23. 23. The method of claim 22, wherein the autoimmune or inflammatory disease is selected from rheumatoid arthritis, inflammatory bowel diseases such as Crohn's disease and ulcerative colitis, psoriasis, hidradenitis suppurativa, and graft-versus-host disease.

24. Use of a polypeptide according to any one of claims 1 to 13 or a composition according to claim 17 or 18 in the preparation of a pharmaceutical composition for treating an autoimmune or inflammatory disease.

25. 25. Use of a polypeptide or composition according to claim 24, wherein the autoimmune or inflammatory disease is selected from rheumatoid arthritis, inflammatory bowel diseases such as Crohn's disease and ulcerative colitis, psoriasis, hidradenitis suppurativa, graft-versus-host disease.

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