Antibacterial and antiviral agent and disinfectant for prevention of livestock infectious diseases

Abstract

To provide an agent capable of giving quicklime for epidemic prevention used in "standby disinfection" both rapid and lasting disinfecting effects, and to provide a novel disinfectant for epidemic prevention obtained by using the agent together with quicklime for epidemic prevention.SOLUTION: An antibacterial and antiviral agent comprises a chlorite-supported inorganic porous material (A) having chlorite supported on an inorganic porous body, and lauryl sulfate (B).SELECTED DRAWING: None

Description

[Technical Field]

[0001] The present invention relates to an antibacterial and antiviral agent, an additive for slaked lime for epidemic prevention, a disinfectant for livestock infectious disease prevention, and a disinfection method for livestock infectious disease prevention. [Background technology]

[0002] In recent years, livestock infectious diseases such as foot-and-mouth disease, avian influenza, and swine fever have caused enormous damage all over the world. In Japan, to prevent livestock infectious diseases, a "waiting disinfection" method is implemented in which powdered slaked lime is sprayed at farm entrances and inside and outside livestock barns. Slaked lime becomes highly alkaline in the presence of water, and its high pH damages cell membranes, and it breaks, denatures, and inactivates ionic bonds in proteins, resulting in a disinfectant effect against a wide range of pathogens, including viruses and bacteria. Powdered and granular slaked lime can be spread directly on the ground, such as on soil or pavement, eliminating the need for a disinfection tank and making it easy to spread inside and outside farm entrances and livestock barns. Furthermore, slaked lime ultimately reacts with carbon dioxide in the environment to turn into safe calcium carbonate, meaning it has a smaller environmental impact than other disinfectants, offering numerous advantages.

[0003] The slaked lime used in "standby disinfection" reacts with carbon dioxide in the air or rainwater to turn into calcium carbonate, which lowers the pH. As a result, the disinfecting effect of calcium carbonate is greatly reduced, but the problem is that this deterioration is difficult to determine visually. Furthermore, since hydrated lime does not have a disinfecting effect against microorganisms when dry, there is also the problem that it is impossible to determine by visual inspection how effective the hydrated lime will be after it has been spread. To address these problems, methods have been proposed, such as using granulated materials containing slaked lime to suppress the decline in disinfection effect, and adding a chemical that changes color depending on the pH, such as thymol blue, to visually confirm the decline in disinfection effect (Patent Document 1, etc.).However, these methods do not solve the essential problem that the disinfection effect of slaked lime is highly dependent on the environment in which it is used, and no satisfactory proposals have been made for suppressing the decline in disinfection effect. [Prior art documents] [Patent documents]

[0004] [Patent Document 1] Japanese Patent Application Publication No. 2018-039767 Summary of the Invention [Problem to be solved by the invention]

[0005] The present invention aims to provide an agent that imparts immediate and sustained disinfecting effects to epidemic prevention slaked lime used in "anticipatory disinfection," and a new epidemic prevention disinfectant that uses the agent in combination with epidemic prevention slaked lime. [Means for solving the problem]

[0006] As a result of intensive research into solving the above problems, the present inventors have found that a combination of a chlorite-supported inorganic porous material and a specific anionic surfactant exhibits a synergistic antibacterial and antiviral activity against viruses, bacteria, and the like, which is greater than or equal to the additive effect that either material alone cannot achieve. They have also found that adding an antibacterial and antiviral agent comprising this combination to epidemic prevention slaked lime can achieve an immediate and sustained disinfecting effect of the epidemic prevention slaked lime, and have thus completed the present invention.

[0007] The present invention specifically relates to the following items. 1. A chlorite-supported inorganic porous material (A) in which chlorite is supported on an inorganic porous material, and Contains lauryl sulfate (B) Antibacterial and antiviral agent. 2. The antibacterial and antiviral agent according to 1., further comprising a desiccant (C). An additive to slaked lime for epidemic prevention, containing the antibacterial and antiviral agent described in 3.1. 4. An antibacterial and antiviral agent according to 1. or 2. and hydrated lime for epidemic prevention, Disinfectant for preventing infectious diseases in livestock. 5.4. The disinfectant for preventing infectious diseases in livestock, A disinfection method for preventing infectious diseases in livestock, characterized by spraying or applying the disinfectant inside and / or around livestock houses. [Effects of the Invention]

[0008] The antibacterial and antiviral agent of the present invention, which is a combination of a chlorite-supported inorganic porous material and a specific anionic surfactant, exhibits excellent effects in that it exhibits a synergistic antibacterial and antiviral activity against viruses, bacteria, etc., which is greater than or equal to the additive effect that each agent alone cannot achieve. Furthermore, adding this antibacterial and antiviral agent to epidemic prevention slaked lime gives the slaked lime an immediate disinfecting effect and significantly improves the durability of the disinfecting effect, making it possible to reduce the number of times the slaked lime used for "anticipatory disinfection" needs to be sprayed / applied and also to significantly reduce the amount of slaked lime used, which is extremely useful for livestock infectious disease prevention. [Brief explanation of the drawings]

[0009] [Figure 1] 1 is a graph showing the results of "Test 1: indoor placement + no watering" in the "Test to confirm the pH-independent antibacterial effect of disinfectants for preventing infectious diseases in livestock" in the Examples, showing the antibacterial rate (%) on the day of placement and 3, 6, and 8 weeks after placement, and the pH values ​​on the day of placement and 3, 4, 6, and 8 weeks after placement. [Figure 2] 1 is a graph showing the antibacterial rate (%) and pH value on the day of placement, and 9, 12, 15, and 18 days after placement, as a result of "Test 3: Placed indoors with watering" in the "Test to confirm the pH-independent antibacterial effect of disinfectants for preventing infectious diseases in livestock" in the Examples. [Figure 3] 1 is a graph showing the TCID50 / mL of test sample a and the negative control as a result of "Test 1: Placed indoors + No watering" in the "Test to confirm the inactivation of highly pathogenic avian influenza virus by disinfectants for livestock infectious disease prevention" in the Examples. [Figure 4] 1 is a graph showing the TCID50 / mL of test samples b and c and the negative control as a result of "Test 2: indoor placement + no watering, outdoor placement at a pig farm" in the "Test to confirm the inactivation of highly pathogenic avian influenza virus by disinfectants for livestock infectious disease prevention" in the Examples. DETAILED DESCRIPTION OF THE INVENTION

[0010] The present invention will be described in detail below. <Component (A)> The antibacterial and antiviral agent of the present invention comprises, as component (A), a chlorite-supported inorganic porous material in which chlorite is supported on an inorganic porous material. Chlorite salts such as sodium chlorite are known to have the risk of ignition due to their nature, such as by impact, and are internationally registered as oxidizing substances.By supporting chlorite salt on the inorganic porous body in the present invention, the risk of ignition and the like is reduced, and it is no longer necessary to treat it as an oxidizing solid, making it easier to handle as a substance.In addition, chlorite salts are deliquescent, and since they absorb moisture from the outside air and deliquesce, it has been difficult to use them for long periods of time in an environment exposed to the outside air.However, by supporting chlorite salt on the inorganic porous body and using it as component (A), it becomes possible to use it in an open air environment.

[0011] It is known that chlorite's bactericidal power is first exerted when the pH of its aqueous solution is adjusted to 4 using an acid, and that this bactericidal power is maximized at a pH of 2. This is thought to be because, when the pH of a chlorite aqueous solution is adjusted to 4 or less, chlorine dioxide, chloric acid, and hydrochloric acid are produced in the solution, and the amount of chlorine dioxide produced, which is considered to be the main form of sterilization, is greatest in a pH 2 solution. The antibacterial and antiviral agent of the present invention does not contain any acidic substances, is neutral to slightly alkaline when suspended in water, and does not produce any confirmed chlorine dioxide, yet still exhibits antibacterial and antiviral activity. The mechanism by which the antibacterial and antiviral agent of the present invention exhibits antibacterial and antiviral activity is unknown, but by using components (A) and (B) in combination, antibacterial and antiviral activity is exhibited even in the presence of component (C). Furthermore, it has now been confirmed for the first time that the exhibited antibacterial and antiviral activity exhibits a synergistic effect that is greater than or equal to the additive effect compared to the antibacterial and antiviral activity of components (A) and (B) alone.

[0012] The chlorite that can be used in the present invention can be, for example, alkali metal chlorite and alkaline earth metal chlorite.As alkali metal chlorite, for example, sodium chlorite, potassium chlorite, lithium chlorite can be included, and as alkaline earth metal chlorite, for example, calcium chlorite, magnesium chlorite, barium chlorite can be included.Among them, alkali metal chlorite is preferred because it is widely used and low-cost, and sodium chlorite is more preferred. The inorganic porous material in the present invention is not particularly limited in type, and any known porous carrier can be used. The inorganic porous body of the present invention is preferably one in which 2.5 to 5 parts by weight of pure water is added to 1 part by weight of the inorganic porous body, and the mixture is shaken to leach the contained components into the water, and the pH value of the leachate measured with a glass electrode is 7 or higher, and an inorganic porous body exhibiting a neutral or alkaline pH of 7.1 or higher is more preferred. The inorganic porous body of the present invention may be a fired body obtained by adding a flux such as sodium carbonate or sodium bicarbonate to a base inorganic porous body and firing the mixture. For example, flux-calcined diatomaceous earth, which is obtained by adding a flux to diatomaceous earth and firing it, may also be used. The shape of the inorganic porous material in the present invention is not particularly limited and can be arbitrarily selected from granular, powdery, fibrous, etc. Generally, granular or powdery materials are easily available and inexpensive, and therefore can be preferably used. The granular or powdered inorganic porous material preferably has an average particle size in the range of about 0.01 to 10 mm, more preferably 0.025 to 3 mm, and even more preferably 0.03 to 0.1 mm. Note that the average particle size in this specification refers to the 50% particle size (median diameter). The inorganic porous material of the present invention has a BET specific surface area of ​​10 to 300 m 2 / g range is preferred.

[0013] The method for supporting chlorite on the inorganic porous material of component (A) of the present invention, the chlorite-supported inorganic porous material, is not particularly limited. For example, chlorite is dissolved in a solvent to prepare a chlorite solution, and the inorganic porous material is immersed in the chlorite solution to impregnate it, or the chlorite solution is sprayed onto the inorganic porous material. There are no particular requirements for the spraying method as long as it can be sprayed evenly. A simple method is to uniformly spray the chlorite solution on the inorganic porous material while stirring it in a mixing device. After impregnation or spraying with the chlorite solution, the inorganic porous material is dried to firmly support the chlorite on the inorganic porous material. The drying method is not particularly limited, but examples include drying using a fluidized bed dryer or a tray dryer. The drying conditions are also not particularly limited, but drying is performed at 30 to 130°C, preferably 70 to 100°C, for 0.5 to 48 hours, preferably 3 to 8 hours. By this drying, the moisture content of the entire chlorite-supporting inorganic porous material is preferably 30% by weight or less, more preferably 20% by weight or less, and even more preferably 10% by weight or less. The above-mentioned chlorite solution can be an aqueous solution when the chlorite used is water-soluble, but can also contain an organic solvent that can dissolve the chlorite.In addition, the chlorite concentration is not limited and can be set appropriately, for example, in the case of sodium chlorite aqueous solution, it is preferably about 25 wt%.

[0014] The chlorite-supporting inorganic porous material (component (A)) of the present invention preferably has a chlorite content, i.e., the proportion of chlorite when the total of the inorganic porous material and chlorite is taken as 100% by weight, in the range of 1 to 50% by weight, more preferably 5 to 40% by weight, and even more preferably 10 to 35% by weight. The content of chlorite in the antibacterial and antiviral agent of the present invention is preferably in the range of 1 to 25% by weight, and more preferably in the range of 5 to 20% by weight, when the antibacterial and antiviral agent is taken as 100% by weight. The chlorite-supporting inorganic porous material as component (A) in the present invention is not limited to one type, and two or more chlorite-supporting inorganic porous materials may be used in combination, each combining a different inorganic porous material with a chlorite.

[0015] <Ingredient (B)> The antibacterial and antiviral agent of the present invention contains lauryl sulfate as component (B). Examples of lauryl sulfate include salts of alkali metals, alkaline earth metals, ammonium, and alkanolamines. Of these, sodium and potassium salts are preferred, and sodium salts are more preferred. Sulfate esters of higher alcohols are known to undergo hydrolysis, and lauryl sulfate also undergoes hydrolysis in aqueous solutions in the acidic range.However, it is also known that lauryl sulfate has high alkali resistance and does not undergo hydrolysis at all in aqueous solutions in the alkaline range. The antibacterial and antiviral agent of the present invention exhibits a neutral to slightly alkaline pH when suspended in water, and therefore it is presumed that component (B) in the antibacterial and antiviral agent of the present invention has not been hydrolyzed. The content of component (B) in the antibacterial and antiviral agent of the present invention is preferably in the range of 5 to 85% by weight, more preferably in the range of 15 to 75% by weight, and even more preferably in the range of 25 to 65% by weight, when the entire antibacterial and antiviral agent is taken as 100% by weight.

[0016] <Component (C)> The antibacterial and antiviral agent of the present invention preferably contains a desiccant as component (C) to prevent the unexpected generation of chlorine dioxide during storage or transportation. Component (C) in the present invention is a component that is incorporated for the purpose of absorbing moisture (water) from the antibacterial and antiviral agent and its environment before use, thereby improving storage stability. Examples of the component (C) of the present invention include (anhydrous) magnesium sulfate, silica gel, and zeolite. Crystalline zeolite is preferred as the zeolite. As the component (C) of the present invention, one or more of these desiccants can be used in combination. When (anhydrous) magnesium sulfate is used as component (C) of the present invention, (anhydrous) magnesium sulfate may be used alone or in combination with one or more other desiccants. However, an embodiment in which (anhydrous) magnesium sulfate is used in combination with one or more other desiccants is preferred, and among these, a configuration in which (anhydrous) magnesium sulfate and silica gel are contained as component (C) is preferred. When component (C) is contained in the antibacterial and antiviral agent of the present invention, the content thereof is preferably in the range of 5 to 35% by weight, more preferably in the range of 7 to 30% by weight, and even more preferably in the range of 10 to 30% by weight, when the entire antibacterial and antiviral agent is taken as 100% by weight.

[0017] <Preparation of antibacterial and antiviral agents> The antibacterial and antiviral agent of the present invention can be prepared by weighing out component (A) and component (B), and optionally component (C), in predetermined proportions and mixing them. Any mixing method can be used as long as it can achieve uniform mixing, and examples include mixing methods using a V-type mixer or a container rotation and shaking mixer. The mixing time depends on the amount to be mixed and the performance of the mixer used, but for example, when using a container rotation and shaking mixer, a mixing time of 20 to 40 minutes is appropriate for mixing 100 kg. The antibacterial and antiviral agent of the present invention may be prepared in the form of powder or granules, or may be tableted and molded into a cylindrical shape, or may be molded into other dosage forms.

[0018] <Additive for slaked lime for epidemic prevention> The antibacterial and antiviral agent of the present invention, in an embodiment containing component (A) and component (B) or components (A) to (C), can be used as an additive to slaked lime for epidemic prevention. By using the antibacterial and antiviral agent of the present invention as an additive to antimicrobial slaked lime, the antimicrobial slaked lime can be given an immediate disinfecting effect and the durability of the disinfecting effect can be significantly improved. By using the antibacterial and antiviral agent of the present invention as an additive to epidemic slaked lime, it is possible to reduce the number of times the epidemic slaked lime used for "anticipatory disinfection" is sprayed / applied, and also to significantly reduce the amount of epidemic slaked lime used. The shape of the slaked lime for epidemic prevention is not particularly limited, and examples include powder, pellets, etc., but powder form is preferable for spreading it in and / or around livestock houses, and mixing it with water to form a suspension is also preferable for applying it in and / or around livestock houses. When the antibacterial and antiviral agent of the present invention is used as an additive to antimicrobial slaked lime, it is preferably added in an amount of 1 to 20 parts by weight, more preferably 1 to 15 parts by weight, even more preferably 1 to 10 parts by weight, and particularly preferably 1 to 5 parts by weight, per 100 parts by weight of antimicrobial slaked lime.

[0019] <Disinfectant for preventing infectious diseases in livestock> The term "calcium hydroxide for epidemic prevention" as used herein refers to slaked lime for epidemic prevention purposes that is widely used as a standby disinfectant in livestock farming sites. Calcium hydroxide, the main component of slaked lime, exhibits strong alkalinity in the presence of water, thereby exerting a disinfecting effect in preventing infectious diseases in livestock. Here, anticipated disinfection refers to the creation of a slaked lime zone with slaked lime powder at the entrance to a farm or inside and outside a livestock barn, with the aim of inactivating pathogens that are attached to vehicles passing through, the soles of people's shoes, or the feet and bodies of wild animals. Normally, slaked lime for epidemic prevention, which does not deteriorate, has a pH of about 13. However, when exposed to carbon dioxide in the atmosphere or rain containing dissolved carbon dioxide, calcium hydroxide gradually changes to calcium carbonate, lowering the pH and weakening the alkalinity, which reduces the disinfecting effect. This is a weakness. The disinfectant for preventing infectious diseases in livestock of the present invention is a combination of the antibacterial and antiviral agent containing the component (A) and component (B) of the present invention, and optionally component (C), and disinfectant slaked lime. This combination exerts the effect of imparting the immediate disinfecting effect of the disinfectant slaked lime and significantly improving the durability of the disinfectant effect.

[0020] In the disinfectant for preventing infectious diseases in livestock of the present invention, the antibacterial and antiviral agent of the present invention is added in an amount of preferably 1 to 20 parts by weight, more preferably 1 to 15 parts by weight, even more preferably 1 to 10 parts by weight, and particularly preferably 1 to 5 parts by weight, per 100 parts by weight of preventive slaked lime. The shape of the disinfectant for preventing infectious diseases in livestock of the present invention is not particularly limited, and examples thereof include powder, granular, pellet, etc., but a powder form is preferable for spreading on the floors of livestock houses such as cowsheds, pig houses, and chicken houses, or on the ground around livestock houses, on the outer edges of farms, etc., and a slurry (suspension) form mixed with water is preferable for applying to walls and pillars inside livestock houses, gutters around livestock houses, and warehouse walls. The disinfectant for livestock infectious disease prevention of the present invention can be sprayed or applied to vehicles, fixtures, roads, premises, feed tanks, fences, the outer surfaces of water supply and distribution pipes, the entire perimeter of a building, roofs, structures surrounding a building or a protected area, the paved surrounding area surrounding a building or a protected area, or the protected area, thereby preventing a wide range of pathogens such as viruses and bacteria from adhering to vehicles or fixtures and spreading, moving across roads or premises, spreading through the outer surfaces of premises, feed tanks, fences, and water supply and distribution pipes, and even invading buildings or areas to be protected, and can exert a disinfecting effect against a wide range of pathogens such as viruses and bacteria. [Example]

[0021] The present invention will be specifically described below using examples and comparative examples. <Test to confirm the effectiveness of antibacterial and antiviral agents> (Preparation of component (A)) (1) Inorganic porous material As an inorganic porous material, it has an average particle size of 7 μm and a BET specific surface area of ​​20 m 2 2.5 parts by weight of pure water was added to 1 part by weight of diatomaceous earth (1 / g) and the mixture was shaken for 30 minutes. The pH value of the leachate was measured with a glass electrode and found to be 8. (2) Chlorite solution As a chlorite solution, a 25 wt % aqueous solution of sodium chlorite was prepared. (3) Preparation of chlorite-supported inorganic porous material 100 parts by weight of the diatomaceous earth (1) was sprayed with 100 parts by weight of an aqueous sodium chlorite solution to impregnate the diatomaceous earth, and then dried at 70°C for 6 hours to obtain a chlorite-supported inorganic porous material (A). The sodium chlorite content in the obtained chlorite-supporting inorganic porous material (A) was 55% by weight. The water content in the chlorite-supporting inorganic porous material (A) was less than 5% by weight.

[0022] (Preparation of test specimens) The test specimen for Comparative Example 1 was prepared by mixing 64 parts by weight of the chlorite-supported diatomaceous earth (A) obtained in the above "Preparation of component (A)", 34 parts by weight of silica gel, and 3 parts by weight of magnesium sulfate (C) in a container rotary rocking mixer for 20 minutes. The test sample of Comparative Example 1 and sodium lauryl sulfate (B) were mixed in a container rotary-shaking mixer for 20 minutes at a weight ratio of 1:1 to prepare the test sample of Example 1. The test sample of Example 1 is a specific example of the antibacterial and antiviral agent of the present invention, containing chlorite-supported diatomaceous earth (A), sodium lauryl sulfate (B), silica gel, and magnesium sulfate (C). The test specimen of Comparative Example 2 contained only sodium lauryl sulfate (B).

[0023] (Test Method) General live bacteria collected from the environment were selectively cultured in a medium for Staphylococcus aureus (Shimadzu Diagnostics Co., Ltd., Compact Dry TC), and the grown colonies were cultured and the number of bacteria was measured. After that, the number of bacteria was reduced to 10 using physiological saline. 6 The solution was diluted to about 100% and designated as bacterial solution A. The test samples of Example 1 and Comparative Examples 1 and 2 were diluted with purified water to test sample concentrations of 1.0 wt%, 0.5 wt%, 0.1 wt%, and 0.05 wt%, and 1 mL of the bacterial solution A was added and allowed to stand for 1 hour (25°C). This was then inoculated onto a general viable culture medium (Shimadzu Diagnostics Co., Ltd., Compact Dry TC) and cultured at 37°C for 48 hours, after which the number of grown colonies was counted (Funakoshi Co., Ltd. Counterpen). The results are summarized in Table 1 below. The values ​​in Table 1 represent the number of grown colonies (cfu / mL).

[0024] [Table 1]

[0025] As shown in Table 1, the test sample of Example 1, which is a specific example of the antibacterial and antiviral agent of the present invention and contains components (A) to (C), was confirmed to exhibit excellent antibacterial activity against Staphylococcus aureus. Moreover, at all test sample concentrations (0.05 to 1.0 wt%), the antibacterial activity exhibited by the test sample of Example 1 was comparable to that expected from the antibacterial activities exhibited by the test samples of Comparative Examples 1 and 2. It was also confirmed that the synergistic effect exceeded the additive effect. The test samples of Example 1 and Comparative Examples 1 and 2 were separately checked for the generation of chlorine dioxide using a gas detection tube method, but no generation of chlorine dioxide was confirmed in any of them, so it is clear that the antibacterial activity is not due to chlorine dioxide.

[0026] <Testing the effectiveness of disinfectants for preventing infectious diseases in livestock> (Test specimen) The test sample of Example 1 in the above "Test to confirm the effectiveness of antibacterial and antiviral agents" was used. The test specimen of Example 2 was prepared by mixing 3% by weight of the test specimen of Example 1 with 97% by weight of antimicrobial slaked lime. The test specimen of Comparative Example 3 contained only slaked lime for epidemic prevention. (Test Method) The test samples of Examples 1 and 2 and Comparative Example 3 were suspended in purified water to concentrations of 1.0 wt % and 0.03 wt %, respectively, and the antibacterial activity was confirmed in the same manner as in the test method described above in "Test to confirm the effectiveness of antibacterial and antiviral agents." The results of antibacterial activity and the pH of a 1.0 wt% purified water suspension of each test sample are summarized in Table 2. Values ​​other than the pH value in Table 2 represent the number of grown colonies (cfu / mL), and "-" indicates that the test was not performed.

[0027] [Table 2]

[0028] The test sample of Example 2, which is a specific example of the disinfectant for preventing infectious diseases in livestock according to the present invention, contains 3% by weight of the test sample of Example 1 and 97% by weight of preventive slaked lime. From the results shown in Table 2, it is considered that the antibacterial activity of the antimicrobial slaked lime in the test sample of Example 2 is roughly equivalent to the antibacterial activity of Comparative Example 3 in which the test sample concentration is 1.0 wt %, and furthermore, it is considered that the antibacterial activity of the test sample of Example 1 in the test sample of Example 2 is roughly equivalent to the antibacterial activity of Example 1 in which the test sample concentration is 0.03 wt %. Therefore, it was revealed that the test sample of Example 2, which is a specific example of the disinfectant for preventing infectious diseases in livestock according to the present invention, exhibits a synergistic antibacterial activity that exceeds the additive effect expected from the combination of the antibacterial and antiviral agent of the present invention and hydrated lime for prevention of epidemics. The test samples of Examples 1 and 2 and Comparative Example 3 were separately checked for the generation of chlorine dioxide using a gas detection tube method, but no generation of chlorine dioxide was confirmed in any of them, so it is clear that the antibacterial activity is not due to chlorine dioxide.

[0029] <Outdoor effectiveness verification test of disinfectants for livestock infectious disease prevention> (Test specimens and specimens) The test samples used were those of Example 2 and Comparative Example 3, which were used in the above-mentioned "Test to confirm the effectiveness of disinfectants for preventing infectious diseases in livestock." The test specimens of Example 2 and Comparative Example 3 were placed on a plywood board (40 cm x 60 cm) at a pressure of 1 kg / m 2 The powder was sprayed as follows: In addition, the test specimens of Example 2 and Comparative Example 3 were suspended in water to form a slurry with a concentration of 25% by weight, and this was applied to a plywood board (40 cm x 60 cm) at a concentration of 1 kg / m 2 (Example 2 is 0.25 kg / m 2 The specimen was applied so that the thickness was 1 / 3 of the original thickness.

[0030] (Test Method) Sample A (powder) and sample B (suspension) were placed outdoors in the wind and rain. Samples were taken from the surface of specimen A (powder) and specimen B (suspension) 2, 10, 20, 30, 45, 50, and 60 days after placement. 1 g of the collected sample was added to a mixture of 1 mL of bacterial solution A from the above "Test to confirm the effectiveness of antibacterial and antiviral agents" and 98 mL of purified water, and after stirring for 1 minute, the mixture was left to stand for 1 hour (25°C). This was then inoculated onto a general viable culture medium and cultured at 37°C for 48 hours, after which the number of grown colonies was counted. The results are summarized in Table 3 below. The values ​​in Table 3 are the number of grown colonies (cfu / mL), and "300<" means that the number of grown colonies could not be measured.

[0031] [Table 3]

[0032] The test sample of Example 2, which is a specific example of the disinfectant for preventing infectious diseases in livestock according to the present invention, contains the test sample of Example 1, i.e., 3% by weight of the antibacterial and antiviral agent of the present invention containing components (A) to (C), and 97% by weight of preventive slaked lime. In the test sample of Comparative Example 3, which consisted only of antimicrobial slaked lime, the number of grown colonies (cfu / mL) did not become "0" two days after being placed outdoors, and the antimicrobial activity began to decline as early as the second day, and complete antimicrobial activity was not achieved. Furthermore, after 10 days, the number of grown colonies became impossible to measure, and it was confirmed that the antimicrobial activity had rapidly diminished. The results after 10 days for Comparative Example 3 are consistent with the phenomenon that the slaked lime used for anti-epidemic disinfection reacts with carbon dioxide in the atmosphere or rainwater to turn into calcium carbonate, and this change significantly reduces the disinfection effect. In contrast, the test specimen of Example 2, which contained 3% by weight of the test specimen of Example 1 containing components (A) to (C) of the present invention and 97% by weight of antimicrobial slaked lime, maintained a completely bacteria-free state for about one month even when placed outdoors, and furthermore, it was revealed that high antimicrobial activity was maintained for 60 days. Although the mechanism of this high antimicrobial activity is currently unknown, as shown in Tables 1 to 3, it is thought to be due not only to the high pH but also to the interaction of components (A) to (C). The pH of 1 wt % purified water suspensions of samples of specimen A (powder) and specimen B (suspension) from Example 2 and samples of specimen A (powder) and specimen B (suspension) from Comparative Example 3 30 and 60 days after placement was as follows: specimen A (powder) from Example 2 was pH 12.3 (after 30 days) and pH 11.4 (after 60 days), and specimen B (suspension) was pH 11.5 (after 30 days) and pH 10.6 (after 60 days), while specimen A (powder) from Comparative Example 3 was pH 10.3 (after 30 days) and pH 10.1 (after 60 days), and specimen B (suspension) was pH 8.8 (after 30 days) and pH 8.1 (after 60 days). As shown in Table 2 of the above "Test to confirm the effectiveness of disinfectants for preventing infectious diseases in livestock," the pH of the 1 wt% purified water suspension before storage was 13.6 for Example 2 and 13.5 for Comparative Example 3, and the pH of both Example 2 and Comparative Example 3 decreased after storage for 30 and 60 days. As mentioned above, the antibacterial mechanism of action of hydrated lime is pH-dependent, and it has been reported that a pH of 12.5 or higher is required. The pH of samples A (powder) and B (suspension) in Example 2 was lower than 12.5 both 30 and 60 days after placement, and therefore, based on conventional technical knowledge, it would be expected that the antibacterial activity would be significantly reduced. However, contrary to previous expectations, it has now been revealed for the first time that high antibacterial activity is maintained. The mechanism behind this result is currently unknown. However, it was revealed that the test sample of Example 2, which is a specific example of a disinfectant for preventing livestock infectious diseases of the present invention, contains 3% by weight of the test sample of Example 1, i.e., the antibacterial and antiviral agent of the present invention containing components (A) to (C), and thereby exhibits two extremely significant effects: (I) although the pH decreases even when placed outdoors, the rate of decrease is slowed, and (II) high antibacterial activity is maintained.

[0033] <Confirmation test of pH-independent antibacterial effect of disinfectants for livestock infectious disease prevention> (Test specimens and specimens) The test samples used were those of Example 2 and Comparative Example 3, which were used in the above-mentioned "Test to confirm the effectiveness of disinfectants for preventing infectious diseases in livestock." The test specimens of Example 2 and Comparative Example 3 were suspended in water to form a slurry with a concentration of 25% by weight, and this was placed on a resin tray (30 cm x 45 cm) at a concentration of 1 kg / m 2 The specimen was sprayed with the amount of spray and dried, and was designated as specimen C.

[0034] (Test 1: Indoor placement + no watering) Specimen C was placed in an "indoor" location that was exposed to sunlight, had a roof and walls, was protected from rain, had no air conditioning, and was exposed to sunlight and wind from outside. On the day of placement and 3, 6, and 8 weeks after placement, samples were collected from the surface of specimen C and ground into a fine powder in an agate mortar. 0.3 g was weighed into a 5 mL microtube, stirred for 10 seconds in a vortex mixer, and left to stand for 30 minutes. To this, 1 mL of bacterial solution A from the above "Test to confirm the effectiveness of antibacterial and antiviral agents" was added, stirred for 10 seconds with a vortex mixer, and left to stand for 10 minutes. To this, 1.5 mL of phosphate-buffered saline (PBS) was added, and stirred for 10 seconds with a vortex mixer. This was serially diluted and then inoculated onto a medium for general viable bacteria (Shimadzu Diagnostics, Compact Dry TC) and cultured at 37°C for 48 hours, after which the number A of grown colonies was counted (Funakoshi Co., Ltd. Counterpen). As a control, specimen C without the fine powder sample was used, and the number of grown colonies B was counted in the same manner. The antibacterial rate (%) was calculated using the following formula. Antibacterial rate (%) = (number of growing colonies B - number of growing colonies A) ÷ number of growing colonies B × 100 In addition, samples were taken from the surface of specimen C on the day of placement and 3, 4, 6, and 8 weeks after placement, and 0.1 g of the powder was ground into a fine powder in an agate mortar. 0.1 g of the powder was added to 10 mL of purified water and stirred. The supernatant and suspension were then allowed to stand for 30 minutes, and the pH of each was measured (Horiba, Ltd.) and the average value was calculated. The antibacterial rate (%) obtained as described above on the day of placement and 3, 6, and 8 weeks after placement, and the pH values ​​obtained as described above on the day of placement and 3, 4, 6, and 8 weeks after placement are shown in Figure 1 below.

[0035] (Test 2: Indoor placement with watering) Sample C was placed in an air-conditioned room and exposed to a 1m precipitation source so that the precipitation rate would be 1 mm per hour (the most frequent amount of precipitation based on the distribution of rainfall intensity in Japan) at a frequency of once every three days (the average frequency of rainfall in Japan). 2 1 L of purified water was sprayed onto the sample, and the sample was allowed to air dry. Otherwise, the same procedure as in "Test 1" above was carried out to obtain the antibacterial rate (%) and pH value on the day of placement and 9, 12, 15, and 18 days after placement. This is summarised in Figure 2 below.

[0036] As shown in Figure 1, when the test specimen of Example 2, which is a specific example of the disinfectant for preventing infectious diseases in livestock according to the present invention, and the test specimen of Comparative Example 3, which consists only of preventive slaked lime, were placed "indoors" where they were exposed to sunlight and not rain, no decrease in pH was observed, and it was confirmed that high antibacterial activity was maintained even 8 weeks after placement. As shown in Figure 2, under conditions where the test specimen of Comparative Example 3, which consisted only of hydrated lime for epidemic prevention, was sprayed with the amount of precipitation that occurs most frequently based on the average rainfall frequency in Japan and the distribution of rainfall intensities in Japan, it was revealed that the pH of the test specimen of Comparative Example 3, which consisted only of hydrated lime for epidemic prevention, dropped significantly after 9 days from placement, and that the antibacterial activity also dropped significantly accordingly. On the other hand, the test sample of Example 2, which is a specific example of a disinfectant for preventing livestock infectious diseases of the present invention, showed a decrease in pH under the same watering conditions, but it was confirmed that high antibacterial activity was maintained even 18 days after placement. These results confirmed that the decrease in pH of antimicrobial slaked lime and the resulting significant decrease in antimicrobial activity are largely dependent on the condition of the antimicrobial slaked lime being exposed to water, such as rainfall.

[0037] <Confirmation test for inactivation of highly pathogenic avian influenza virus by disinfectants for livestock infectious disease prevention> This confirmation test was conducted at the Graduate School of Veterinary Medicine, Hokkaido University, a national university corporation. (Materials used) Test virus: Highly pathogenic avian influenza virus: The following H5N1 strain isolated and stored at Hokkaido University was used. H5N1 strain: A / white-tailed eagle / Hokkaido / 22-RU-WTE-2 / 2022 Host cells: Madin-Darby canine kidney (MDCK) cells Negative control: phosphate-buffered saline (PBS) (Test specimens and specimens) The test sample used was the same as in Example 2 used in the above "Test to confirm the effectiveness of disinfectants for preventing infectious diseases in livestock." The test specimen of Example 2 was suspended in water to form a slurry with a concentration of 20% by weight, and this was placed on a resin tray (30 cm x 45 cm) at a concentration of 1 kg / m2 The specimen was applied in the amount of 1000 and dried, and was designated as specimen D. The test specimen of Example 2 was suspended in water to form a slurry with a concentration of 20% by weight, and this was applied at a rate of 1 kg / m to the surface of a PVC pipe (diameter 25 cm, height 10 cm) on which asphalt had been poured and solidified. 2 The specimen was sprayed with the amount of 100 ml and dried, and the result was designated as specimen E.

[0038] (Test 1: Indoor placement with watering) The specimen D was placed in an air-conditioned room and exposed to a 1m precipitation source so that the precipitation rate would be 1 mm per hour (the most frequent amount of precipitation based on the distribution of rainfall intensity in Japan) at a frequency of once every three days (the average frequency of rainfall in Japan). 2 1 L of purified water was sprayed onto the sample, and the sample was allowed to air dry. Samples were taken from the surface of specimen D on the day of placement and 12, 18, and 24 days after placement, and ground into a fine powder in an agate mortar. This sample was designated test sample a and subjected to the "Highly pathogenic avian influenza virus inactivation test" described below.

[0039] (Preparation of virus suspension) Embryonated chicken eggs were infected with the test virus and cultured at 35°C for 48 hours, after which the allantoic fluid was collected and diluted 5-fold with phosphate-buffered saline (PBS) to prepare the test virus suspension for use in the test. (Inactivation test of highly pathogenic avian influenza virus) 0.3 g of test sample a was mixed with 1.0 mL of distilled water and left to stand for 30 minutes. 0.5 mL of test virus suspension was added and mixed, and then the mixture was allowed to react at 25°C for 10 minutes. 0.04 mL of the reaction solution was mixed with 1.96 mL of horse serum (Thermo Fisher Scientific Inc.) to stop the reaction. The supernatant of the reaction solution was collected and used as a sample for measuring the virus infectivity titer, and the virus infectivity titer was measured. (Virus infectivity titer measurement method: 50% tissue culture infectious dose (TCID 50 )Measurement method) The above sample for measuring the viral infectivity titer was serially diluted 10-fold with Eagle's minimal essential medium (EMEM, Shimadzu Diagnostics Co., Ltd.), and 0.1 mL of each was added to four wells of a 96-well microplate in which MDCK cells had been cultured in advance. After 1 hour, the solution added to the cells was removed, and 0.1 mL of fresh EMEM was added to each well. These MDCK cells were cultured at 35°C for 48 hours. (Determine whether or not the virus is multiplying) The cytopathic effect associated with virus proliferation was observed under a microscope to determine whether virus proliferation occurred or not. The amount of infectious virus present in each virus infectivity measurement sample was calculated as TCID per mL of test solution using the Reed and Muench method. 50 The value was calculated as: For the negative control, TCID on the day of placement and 24 days after placement 50 / mL value was calculated. TCID of test sample a and negative control 50 / mL are shown in Figure 3 below.

[0040] (Test 2: Indoor placement without watering, outdoor placement at a pig farm) Similar to the "Placement indoors + no watering" in "Test 1" of the above "Confirmation test for pH-independent antibacterial effect of disinfectants for livestock infectious disease prevention," sample D was placed in an "indoor" location where it was exposed to sunlight, was not exposed to rain due to the presence of a roof and walls, and was not air-conditioned and received sunlight and wind from outside. Samples were taken from the surface of specimen D 1, 40, and 52 days after placement and ground into a fine powder in an agate mortar. This sample was designated test sample b and subjected to the "Highly pathogenic avian influenza virus inactivation test" described above. Furthermore, the above specimen E was placed outdoors near a pig farm (in an environment exposed to rain, wind, and sunlight), and samples were taken from the surface of specimen E 1, 40, and 52 days after placement and ground into a fine powder in an agate mortar. This sample was designated test sample c and subjected to the "inactivation test of highly pathogenic avian influenza virus." In the "Highly pathogenic avian influenza virus inactivation test" above, the TCID 50 The value was calculated as / mL. For the negative control, TCID on the day of placement and 24 days after placement 50 / mL value was calculated. TCID of test samples b, c and negative control 50 / mL are shown in Figure 4 below.

[0041] As shown in Figure 3, the test sample of Example 2, which is a specific example of the disinfectant for livestock infectious disease prevention of the present invention, inactivated 99.9% or more of highly pathogenic avian influenza viruses even under water spray conditions and even 24 days after placement, demonstrating a high virus inactivation effect. Furthermore, it was confirmed that the test sample of Example 2, which is a specific example of the disinfectant for preventing infectious diseases in livestock according to the present invention, exhibits a similar high virus inactivation effect when used in powder form. As shown in Figure 4, the test sample of Example 2, which is a specific example of the disinfectant for livestock infectious disease prevention of the present invention, exhibited a high virus inactivation effect even 8 weeks after placement when placed "indoors" where it was exposed to sunlight and not rain.Furthermore, even when used outdoors around a pig farm, which is the same method as "anticipatory disinfection," it was confirmed to inactivate more than 99.9% of highly pathogenic avian influenza viruses even 52 days after placement, despite being exposed to rain, wind, and sunlight, and to exhibit a high virus inactivation effect. These results confirmed that the decrease in pH of antimicrobial slaked lime and the resulting significant decrease in antimicrobial activity are largely dependent on the condition of the antimicrobial slaked lime being exposed to water, such as rainfall.

[0042] The disinfectant for preventing infectious diseases of livestock according to the present invention, by adding an extremely small amount of the antibacterial and antiviral agent of the present invention, such as 3% by weight, to preventive slaked lime, solves the drawbacks of standby disinfection using slaked lime, namely, that the pH decreases due to the influence of the outside air environment, thereby reducing antibacterial activity, and further, that the timing at which the antibacterial activity decreases is also dependent on the outside air environment. It has been revealed that the disinfectant is less affected by the outside air environment, imparts an immediate disinfecting effect to the preventive slaked lime, and greatly improves the durability of the disinfecting effect. The disinfectant for livestock infectious disease prevention of the present invention has the effect of greatly improving the durability of the disinfecting effect of preventive slaked lime, thereby making it possible to reduce the number of times the preventive slaked lime is sprayed / applied for use in "anticipatory disinfection" and also to significantly reduce the amount of preventive slaked lime used, making it extremely useful for livestock infectious disease prevention.

[0043] <Reference exam> (Preparation of reference test samples and reference comparison test samples) To a mixture of 2 parts by weight of the chlorite-supported diatomaceous earth (A) obtained in the "Preparation of component (A)" in the "Test to confirm the effectiveness of antibacterial and antiviral agents" above, 2 parts by weight of N-lauroyl sarcosine, and 97 parts by weight of disinfectant slaked lime, 2,400 parts by weight of water was added and stirred to obtain a reference test sample in the form of a slurry of 2,500 parts by weight in total. In addition, in the above-mentioned "Test to confirm the effectiveness of disinfectants for preventing infectious diseases in livestock," 2,400 parts by weight of water was added to 100 parts by weight of the test sample of Comparative Example 3, which contained only preventive slaked lime, and the mixture was stirred to obtain a reference comparative test sample in the form of a slurry of 2,500 parts by weight in total. (Test Method) 6.5 g of each of the reference test sample and the reference comparative test sample was applied to petri dishes (diameter 9 cm), and 6 of each were prepared, for a total of 12 dishes. The entire amount of powder was collected from one of the petri dishes that had been coated with the reference test sample or the reference comparative test sample and dried, and this was designated as the "0th" powder sample. 5 mL of purified water was added to the other petri dishes, and the dried powder was collected from one of them the next day to three days later, and this was designated as the "1st" powder sample. Next, water addition and drying were repeated to obtain "2nd to 6th" powder samples. After water addition, each petri dish was stored in an open state at room temperature (approximately 20°C). The above water addition was intended to simulate rainfall. The antibacterial activity of the collected powder samples was confirmed as follows. 0.5 mL of purified water was added to 0.3 g of powdered sample, and the mixture was stirred and mixed using a vortex mixer, and then allowed to stand for 30 minutes. To this was added 1 mL of bacterial solution A from the above-mentioned "Test to confirm the effectiveness of antibacterial and antiviral agents," and the mixture was stirred for 10 seconds using a vortex mixer. After leaving the mixture to stand for 10 minutes, 0.11 mL of the mixture was measured out, and 1.0 mL of phosphate-buffered saline (PBS) was added, followed by stirring for 10 seconds using a vortex mixer. This was serially diluted and then inoculated onto a general viable culture medium (Shimadzu Diagnostics, Compact Dry TC) and cultured at 37°C for 48 hours, after which the number of grown colonies was counted (Funakoshi Co., Ltd. Counterpen).

[0044] (Test results) The reference test sample, even in the powder sample from the sixth repetition, showed the same antibacterial activity as the powder sample from the zeroth repetition, whereas the reference comparison test sample, even in the powder sample from the third repetition, was confirmed to have antibacterial activity reduced to approximately 1 / 100 to 1 / 1000 of that of the powder sample from the first repetition. These results demonstrate that N-lauroyl sarcosine (salt) as component (B) in the present invention also exhibits excellent effects equivalent to those of lauryl sulfate, and that adding it to antimicrobial slaked lime imparts an immediate disinfecting effect to the antimicrobial slaked lime and significantly improves the durability of the disinfecting effect, thereby reducing the number of times the antimicrobial slaked lime is sprayed / applied for "anticipatory disinfection" and also enabling a significant reduction in the amount of antimicrobial slaked lime used. (1) An antibacterial and antiviral agent comprising (a) a chlorite-supported inorganic porous material in which chlorite is supported on an inorganic porous material, and (b) N-lauroyl sarcosine (salt). (2) An additive for epidemic prevention slaked lime, comprising the antibacterial and antiviral agent described in (1). (3) A disinfectant for livestock infectious disease prevention, comprising the antibacterial and antiviral agent described in (1) or (2) and epidemic prevention slaked lime. (4) A disinfection method for livestock infectious disease prevention, comprising spraying or applying the disinfectant for livestock infectious disease prevention described in (3) to the inside and / or surrounding areas of livestock barns.

Claims

1. A chlorite-supported inorganic porous body (A) in which a chlorite is supported on an inorganic porous body; and Contains lauryl sulfate (B) Antibacterial and antiviral agent.

2. The antibacterial and antiviral agent according to claim 1, further comprising a desiccant (C).

3. An additive to slaked lime for epidemic prevention, comprising the antibacterial and antiviral agent according to claim 1.

4. An antibacterial and antiviral agent according to claim 1 or 2, and slaked lime for epidemic prevention. Disinfectant for preventing infectious diseases in livestock.

5. The disinfectant for preventing infectious diseases in livestock according to claim 4, A disinfection method for preventing infectious diseases in livestock, characterized by spraying or applying the disinfectant in and / or around livestock houses.

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