Antibodies, immunoconjugates, antigen-recognizing receptors, immunoresponsive cells, pharmaceutical compositions, nucleic acids, vectors, host cells, methods, and kits

Abstract

Antibodies and antibody derivatives that bind to OX40 and methods of use thereof are provided. The antibodies or antibody derivatives include single domain antibodies that bind to OX40.

Description

[Technical field]

[0001] (CROSS REFERENCE TO RELATED APPLICATIONS) This application claims priority to International Patent Application No. PCT / CN2021 / 139277, filed December 17, 2021, the contents of which are incorporated by reference in their entirety herein and claim priority thereto.

[0002] The present invention relates to antibodies and antibody derivatives that bind to OX40 and methods of use thereof. [Background technology]

[0003] OX40, also known as tumor necrosis factor receptor superfamily member 4 (Tnfrsf4) and CD134, is a type 1 transmembrane glycoprotein that is mainly expressed by immune cells such as T cells. OX40 can induce the expression of anti-apoptotic proteins and cell cycle progression proteins, thereby inhibiting activation-induced cell death and promoting the survival of antigen-specific memory T cells. OX40 costimulatory signals can further activate the NF-kB pathway to directly stimulate effector T cells. It should be noted that OX40 has been found in tumor-infiltrating lymphocytes (TILs) of various different cancers, including head and neck squamous cell carcinoma, ovarian cancer, gastric cancer, cutaneous squamous cell carcinoma, breast cancer, and colorectal cancer. Previous studies have revealed that activated OX40 and / or its ligand (OX40L) can induce antitumor effects. Therefore, there is a need in the art for the development of OX40-targeting molecules and methods for treating cancer. Summary of the Invention

[0004] The present invention provides isolated monoclonal antibodies and antibody derivatives that specifically bind to OX40 with high affinity, including monospecific anti-OX40 antibodies and multispecific antibodies that bind to OX40 and one or more additional targets. In some embodiments, the antibodies or antibody derivatives disclosed herein include single domain antibodies that bind to OX40. The present invention further provides methods for preparing and using the antibodies and antibody derivatives disclosed herein, and pharmaceutical compositions that include the antibodies and antibody derivatives, for use in treating diseases and disorders such as, for example, cancer. The present invention is based in part on the discovery of novel single domain antibodies that bind to OX40, which can provide improved anti-tumor effects by targeting tumor cells and / or increasing the immune response against tumor cells.

[0005] The present invention provides an antibody that binds to OX40, the antibody comprising a single domain antibody that binds to OX40. In some embodiments, the single domain antibody is at least 1x10 -7 In some embodiments, the single domain antibody binds to OX40 with a KD of 5x10 -8 In some embodiments, the single domain antibody binds to OX40 with a KD of 1x10 -8 In some embodiments, the single domain antibody binds to OX40 with a KD of about 1x10 -10 M to approx. 5x10 -8 It binds to OX40 with a KD of M. In some embodiments, the single domain antibody comprises a VHH. In some embodiments, the single domain antibody or VHH comprises a heavy chain variable region (VH).

[0006] In some embodiments, the single domain antibody cross-competes for binding to OX40 with a reference anti-OX40 single domain antibody comprising a heavy chain variable region comprising: a) a heavy chain variable region CDR1 comprising the amino acid sequence set forth in SEQ ID NO: 1, a heavy chain variable region CDR2 comprising the amino acid sequence set forth in SEQ ID NO: 2, and a heavy chain variable region CDR3 comprising the amino acid sequence set forth in SEQ ID NO: 3; b) a heavy chain variable region CDR1 comprising the amino acid sequence set forth in SEQ ID NO: 6, a heavy chain variable region CDR2 comprising the amino acid sequence set forth in SEQ ID NO: 7, and a heavy chain variable region CDR3 comprising the amino acid sequence set forth in SEQ ID NO: 8; c) a heavy chain variable region CDR1 comprising the amino acid sequence set forth in SEQ ID NO: 11, a heavy chain variable region CDR2 comprising the amino acid sequence set forth in SEQ ID NO: 12, and a heavy chain variable region CDR3 comprising the amino acid sequence set forth in SEQ ID NO: 13; a) a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO: 16, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO: 17, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO: 18; b) a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO: 21, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO: 22, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO: 23; c) a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO: 26, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO: 27, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO: 28; g) a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO: 31, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO: 32, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO: h) a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO: 36, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO: 37, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO: 38; i) a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO: 37, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO: 38.a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO: 41, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO: 42, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO: 43; j) a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO: 46, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO: 47, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO: 48; k) a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO: 51, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO: 52, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO: 53; l) a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO: 56, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO: 57, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO: 58; a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO:61, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO:62, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO:63; n) a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO:66, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO:67, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO:68; o) a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO:71, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO:72, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO:73; or p) a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO:76, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO:77, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO:78.

[0007] In some embodiments, the single domain antibody comprises a heavy chain variable region comprising: a) a heavy chain variable region CDR1 comprising the amino acid sequence of any one of SEQ ID NOs: 1, 6, 11, 16, 21, 26, 31, 36, 41, 46, 51, 56, 61, 66, 71 or 76, or a variant of said amino acid sequence comprising at most about three amino acid substitutions; b) a heavy chain variable region CDR2 comprising the amino acid sequence of any one of SEQ ID NOs: 2, 7, 12, 17, 22, 27, 32, 37, 42, 47, 52, 57, 62, 67, 72 or 77, or a variant of said amino acid sequence comprising at most about three amino acid substitutions; and c) a heavy chain variable region CDR3 comprising the amino acid sequence of any one of SEQ ID NOs: and a heavy chain variable region CDR3 comprising any one of the amino acid sequences of 3, 8, 13, 18, 23, 28, 33, 38, 43, 48, 53, 58, 63, 68, 73 or 78, or a variant of said amino acid sequence comprising at most about three amino acid substitutions.

[0008] In some embodiments, the single domain antibody comprises a heavy chain variable region comprising a CDR1 domain, a CDR2 domain and a CDR3 domain, wherein the CDR1 domain, the CDR2 domain and the CDR3 domain respectively comprise the CDR1 domain, the CDR2 domain and the CDR3 domain comprised in a reference heavy chain variable region, wherein the reference heavy chain variable region comprises an amino acid sequence selected from SEQ ID NO: 4, 9, 14, 19, 24, 29, 34, 39, 44, 49, 54, 59, 64, 69, 74 and 79.

[0009] In some embodiments, the single domain antibody comprises a heavy chain variable region CDR1 comprising the amino acid sequence set forth in SEQ ID NO:1, a heavy chain variable region CDR2 comprising the amino acid sequence set forth in SEQ ID NO:2, and a heavy chain variable region CDR3 comprising the amino acid sequence set forth in SEQ ID NO:3. In some embodiments, the single domain antibody comprises a heavy chain variable region CDR1 comprising the amino acid sequence set forth in SEQ ID NO:6, a heavy chain variable region CDR2 comprising the amino acid sequence set forth in SEQ ID NO:7, and a heavy chain variable region CDR3 comprising the amino acid sequence set forth in SEQ ID NO:8. In some embodiments, the single domain antibody comprises a heavy chain variable region CDR1 comprising the amino acid sequence set forth in SEQ ID NO:11, a heavy chain variable region CDR2 comprising the amino acid sequence set forth in SEQ ID NO:12, and a heavy chain variable region CDR3 comprising the amino acid sequence set forth in SEQ ID NO:13. In some embodiments, the single domain antibody comprises a heavy chain variable region CDR1 comprising the amino acid sequence set forth in SEQ ID NO: 16, a heavy chain variable region CDR2 comprising the amino acid sequence set forth in SEQ ID NO: 17, and a heavy chain variable region CDR3 comprising the amino acid sequence set forth in SEQ ID NO: 18. In some embodiments, the single domain antibody comprises a heavy chain variable region CDR1 comprising the amino acid sequence set forth in SEQ ID NO: 21, a heavy chain variable region CDR2 comprising the amino acid sequence set forth in SEQ ID NO: 22, and a heavy chain variable region CDR3 comprising the amino acid sequence set forth in SEQ ID NO: 23. In some embodiments, the single domain antibody comprises a heavy chain variable region CDR1 comprising the amino acid sequence set forth in SEQ ID NO: 26, a heavy chain variable region CDR2 comprising the amino acid sequence set forth in SEQ ID NO: 27, and a heavy chain variable region CDR3 comprising the amino acid sequence set forth in SEQ ID NO: 28. In some embodiments, the single domain antibody comprises a heavy chain variable region CDR1 comprising the amino acid sequence depicted in SEQ ID NO:31, a heavy chain variable region CDR2 comprising the amino acid sequence depicted in SEQ ID NO:32, and a heavy chain variable region CDR3 comprising the amino acid sequence depicted in SEQ ID NO:33.In some embodiments, the single domain antibody comprises a heavy chain variable region CDR1 comprising the amino acid sequence set forth in SEQ ID NO: 36, a heavy chain variable region CDR2 comprising the amino acid sequence set forth in SEQ ID NO: 37, and a heavy chain variable region CDR3 comprising the amino acid sequence set forth in SEQ ID NO: 38. In some embodiments, the single domain antibody comprises a heavy chain variable region CDR1 comprising the amino acid sequence set forth in SEQ ID NO: 41, a heavy chain variable region CDR2 comprising the amino acid sequence set forth in SEQ ID NO: 42, and a heavy chain variable region CDR3 comprising the amino acid sequence set forth in SEQ ID NO: 43. In some embodiments, the single domain antibody comprises a heavy chain variable region CDR1 comprising the amino acid sequence set forth in SEQ ID NO: 46, a heavy chain variable region CDR2 comprising the amino acid sequence set forth in SEQ ID NO: 47, and a heavy chain variable region CDR3 comprising the amino acid sequence set forth in SEQ ID NO: 48. In some embodiments, the single domain antibody comprises a heavy chain variable region CDR1 comprising the amino acid sequence set forth in SEQ ID NO:51, a heavy chain variable region CDR2 comprising the amino acid sequence set forth in SEQ ID NO:52, and a heavy chain variable region CDR3 comprising the amino acid sequence set forth in SEQ ID NO:53. In some embodiments, the single domain antibody comprises a heavy chain variable region CDR1 comprising the amino acid sequence set forth in SEQ ID NO:56, a heavy chain variable region CDR2 comprising the amino acid sequence set forth in SEQ ID NO:57, and a heavy chain variable region CDR3 comprising the amino acid sequence set forth in SEQ ID NO:58. In some embodiments, the single domain antibody comprises a heavy chain variable region CDR1 comprising the amino acid sequence set forth in SEQ ID NO:61, a heavy chain variable region CDR2 comprising the amino acid sequence set forth in SEQ ID NO:62, and a heavy chain variable region CDR3 comprising the amino acid sequence set forth in SEQ ID NO:63. In some embodiments, the single domain antibody comprises a heavy chain variable region CDR1 comprising the amino acid sequence depicted in SEQ ID NO:66, a heavy chain variable region CDR2 comprising the amino acid sequence depicted in SEQ ID NO:67, and a heavy chain variable region CDR3 comprising the amino acid sequence depicted in SEQ ID NO:68.In some embodiments, the single domain antibody comprises a heavy chain variable region CDR1 comprising the amino acid sequence set forth in SEQ ID NO:71, a heavy chain variable region CDR2 comprising the amino acid sequence set forth in SEQ ID NO:72, and a heavy chain variable region CDR3 comprising the amino acid sequence set forth in SEQ ID NO:73. In some embodiments, the single domain antibody comprises a heavy chain variable region CDR1 comprising the amino acid sequence set forth in SEQ ID NO:76, a heavy chain variable region CDR2 comprising the amino acid sequence set forth in SEQ ID NO:77, and a heavy chain variable region CDR3 comprising the amino acid sequence set forth in SEQ ID NO:78.

[0010] In some embodiments, a single domain antibody comprises a heavy chain variable region comprising an amino acid sequence having at least about 90% sequence identity to an amino acid sequence selected from SEQ ID NOs: 4, 9, 14, 19, 24, 29, 34, 39, 44, 49, 54, 59, 64, 69, 74, and 79. In some embodiments, a single domain antibody comprises a heavy chain variable region comprising an amino acid sequence set forth in SEQ ID NO: 4. In some embodiments, a single domain antibody comprises a heavy chain variable region comprising an amino acid sequence set forth in SEQ ID NO: 9. In some embodiments, a single domain antibody comprises a heavy chain variable region comprising an amino acid sequence set forth in SEQ ID NO: 14. In some embodiments, a single domain antibody comprises a heavy chain variable region comprising an amino acid sequence set forth in SEQ ID NO: 19. In some embodiments, a single domain antibody comprises a heavy chain variable region comprising an amino acid sequence set forth in SEQ ID NO: 24. In some embodiments, a single domain antibody comprises a heavy chain variable region comprising an amino acid sequence set forth in SEQ ID NO: 29. In some embodiments, the single domain antibody comprises a heavy chain variable region comprising the amino acid sequence depicted in SEQ ID NO:34. In some embodiments, the single domain antibody comprises a heavy chain variable region comprising the amino acid sequence depicted in SEQ ID NO:39. In some embodiments, the single domain antibody comprises a heavy chain variable region comprising the amino acid sequence depicted in SEQ ID NO:44. In some embodiments, the single domain antibody comprises a heavy chain variable region comprising the amino acid sequence depicted in SEQ ID NO:49. In some embodiments, the single domain antibody comprises a heavy chain variable region comprising the amino acid sequence depicted in SEQ ID NO:54. In some embodiments, the single domain antibody comprises a heavy chain variable region comprising the amino acid sequence depicted in SEQ ID NO:59. In some embodiments, the single domain antibody comprises a heavy chain variable region comprising the amino acid sequence depicted in SEQ ID NO:64. In some embodiments, the single domain antibody comprises a heavy chain variable region comprising the amino acid sequence depicted in SEQ ID NO:69.In some embodiments, a single domain antibody comprises a heavy chain variable region comprising the amino acid sequence set forth in SEQ ID NO: 74. In some embodiments, a single domain antibody comprises a heavy chain variable region comprising the amino acid sequence set forth in SEQ ID NO: 79. In some embodiments, a single domain antibody comprises a humanized framework.

[0011] In some embodiments, the antibody comprises an Fc region. In some embodiments, the Fc region comprises a human Fc region. In some embodiments, the Fc region comprises an Fc region selected from an IgG, IgA, IgD, IgE, and IgM Fc region. In some embodiments, the Fc region comprises an Fc region selected from an IgG1, IgG2, IgG3, and IgG4 Fc region. In some embodiments, the Fc region comprises an IgG1 Fc region. In some embodiments, the IgG1 Fc region comprises one or more mutations that enhance coengagement with an Fc receptor. In some embodiments, the IgG1 Fc region comprises one or more mutations that enhance coengagement with Fc receptors. In some embodiments, the IgG1 Fc region comprises one or more mutations that enhance coengagement with FcγRIIa, FcγRIIb, or a combination thereof. In some embodiments, the IgG1 Fc region comprises S267E and L328F mutations. In some embodiments, the IgG1 Fc region comprises N325S and L328F mutations.

[0012] In some embodiments, the heavy chain variable region is linked to the Fc region via a linker. In some embodiments, the linker is a peptide linker. In some embodiments, the peptide linker comprises about 4 to about 30 amino acids. In some embodiments, the peptide linker comprises an amino acid sequence selected from SEQ ID NOs: 97-140.

[0013] In some embodiments, the antibody is an agonist antibody. In some embodiments, the antibody binds to domain 2 of a human OX40 polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 95. In some embodiments, the antibody is bivalent, trivalent, tetravalent, pentavalent, hexavalent, heptavalent or octavalent. In some embodiments, the antibody is bivalent. In some embodiments, the antibody is tetravalent. In some embodiments, the antibody is hexavalent.

[0014] In some embodiments, the antibody comprises a heavy chain comprising a VHH domain and an Fc region. In some embodiments, the antibody comprises a heavy chain comprising a VHH domain, a CH1 domain, and an Fc region. In some embodiments, the antibody comprises a light chain comprising a VHH domain and a CL domain. In some embodiments, the antibody comprises a heavy chain comprising an amino acid sequence selected from SEQ ID NOs: 81, 83, 85, and 87. In some embodiments, the antibody comprises a light chain comprising an amino acid sequence selected from SEQ ID NOs: 82, 84, 86, and 88. In some embodiments, the antibody comprises a heavy chain comprising an amino acid sequence set forth in SEQ ID NO: 81 and a light chain comprising an amino acid sequence set forth in SEQ ID NO: 82. In some embodiments, the antibody comprises a heavy chain comprising an amino acid sequence set forth in SEQ ID NO: 83 and a light chain comprising an amino acid sequence set forth in SEQ ID NO: 84. In some embodiments, the antibody comprises a heavy chain comprising an amino acid sequence set forth in SEQ ID NO: 85 and a light chain comprising an amino acid sequence set forth in SEQ ID NO: 86. In some embodiments, the antibody comprises a heavy chain comprising the amino acid sequence set forth in SEQ ID NO:87 and a light chain comprising the amino acid sequence set forth in SEQ ID NO:88. In some embodiments, the antibody comprises a heavy chain comprising the amino acid sequence set forth in SEQ ID NO:81 and a light chain comprising the amino acid sequence set forth in SEQ ID NO:84. In some embodiments, the antibody comprises a heavy chain comprising the amino acid sequence set forth in SEQ ID NO:83 and a light chain comprising the amino acid sequence set forth in SEQ ID NO:82.

[0015] In some embodiments, the antibody comprises a full-length immunoglobulin, a single chain Fv (scFv) fragment, a Fab fragment, a Fab' fragment, a F(ab')2, an Fv fragment, a disulfide stabilized Fv fragment (dsFv), (dsFv)2, a VHH, a VHH-Fc fusion, an Fv-Fc fusion, an scFv-Fc fusion, an scFv-Fv fusion, a diabody, a triabody, a tetrabody, or any combination thereof.

[0016] In some embodiments, the antibody is comprised in a multispecific antibody (e.g., a bispecific antibody), where the multispecific antibody comprises a second antibody portion that specifically binds to a second antigen. In some embodiments, the second antigen is a tumor-associated antigen. In some embodiments, the tumor associated antigen is Her-2, EGFR, PDL1, c-Met, B cell maturation antigen (BCMA), carbonic anhydrase IX (CA1X), carcinoembryonic antigen (CEA), CD5, CD7, CD10, CD19, CD20, CD22, CD30, CD33, CD34, CD38, CD41, CD44, CD49f, CD56, CD74, CD123, CD133, CD138, CD276 (B7H3), epithelial glycoprotein (EGP2), trophoblast cell surface antigen 2 (TROP-2), epithelial glycoprotein-40 (EGP-40), epithelial cell adhesion molecule (EpCAM), receptor tyrosine protein kinase erb-B2, 3, 4, folate binding protein (FBP), fetal acetylcholine receptor (AChR), folate receptor-a, ganglioside G2 (GD2), ganglioside G3 (GD3), human telomerase reverse transcriptase (hTERT), kinase insert domain receptor (KDR), LewisA (CA1.9.9), LewisY (LeY), B7H3, L1 cell adhesion molecule (L1CAM), mucin 16 (Muc-16), mucin 1 (Muc-1), NG2D ligand, oncofetal antigen (h5T4), prostate stem cell antigen (PSCA), prostate specific membrane antigen (PSMA), tumor associated glycoprotein 72 (TAG-72), Claudin 18.2 (CLDN18.2), vascular endothelial growth factor R2 (VEGF-R2), Wilms tumor protein (WT-1), tyrosine protein kinase transmembrane receptor type 1 (ROR1), PVR, PVRL2, GPC3, and any combination thereof. In some embodiments, the second antigen is an immune checkpoint modulator. In some embodiments, the immune checkpoint modulating agent is selected from TIGIT, PD1, CTLA4, LAG-3, 2B4, BTLA, and any combination thereof. In some embodiments, the second antigen is an immune co-stimulatory molecule or a subunit of the T cell receptor / CD3 complex.In some embodiments, the immune co-stimulatory molecule is selected from CD28, ICOS, CD27, 4-1BB, and CD40, and any combination thereof. In some embodiments, the subunit of the T cell receptor / CD3 complex is selected from CD3γ, CD3δ, CD3ε, and any combination thereof.

[0017] The present invention provides immunoconjugates comprising any of the antibodies disclosed herein linked to a therapeutic agent or label. In some embodiments, the therapeutic agent is a cytotoxin or a radioisotope. In some embodiments, the label is selected from a radioisotope, a fluorescent dye, and an enzyme.

[0018] The present invention provides an antigen recognition receptor comprising an extracellular antigen binding domain comprising any of the antibodies disclosed herein. In some embodiments, the antigen recognition receptor is a chimeric antigen receptor (CAR) or a recombinant T cell receptor. In some embodiments, the antigen recognition receptor is a CAR. In some embodiments, the antibody comprised in the extracellular antigen binding domain comprises a VHH.

[0019] The present invention further provides an immunoresponsive cell comprising an antigen recognition receptor disclosed herein. In some embodiments, the immunoresponsive cell is selected from a T cell, a natural killer (NK) cell, a cytotoxic T lymphocyte (CTL), a regulatory T cell, a natural killer T (NKT) cell, and a myeloid cell. In some embodiments, the immunoresponsive cell is a T cell.

[0020] The present invention provides a pharmaceutical composition comprising a) any antibody disclosed herein, any immunoconjugate disclosed herein, or any immunoresponsive cell disclosed herein, and b) a pharma- ceutically acceptable carrier agent.

[0021] The present invention further provides one or more nucleic acids encoding any of the antibodies disclosed herein, one or more vectors comprising any of the nucleic acids disclosed herein, and host cells comprising any of the nucleic acids or vectors disclosed herein.

[0022] The present invention further provides a method for preparing any of the antibodies disclosed herein, the method comprising expressing the antibody in a host cell disclosed herein and isolating the antibody from the host cell.

[0023] The present invention further provides a method of reducing tumor burden in a subject. In some embodiments, the method comprises administering to the subject an effective amount of an antibody disclosed herein, an immunoconjugate disclosed herein, or a pharmaceutical composition disclosed herein. In some embodiments, the method reduces the number of tumor cells. In some embodiments, the method reduces tumor size. In some embodiments, the method eradicates the tumor in the subject. In some embodiments, the tumor exhibits high microsatellite instability (MSI). In some embodiments, the tumor is selected from mesothelioma, lung cancer, pancreatic cancer, ovarian cancer, breast cancer, colon cancer, pleural tumor, glioblastoma, esophageal cancer, gastric cancer, synovial sarcoma, thymic cancer, endometrial cancer, gastric tumor, cholangiocarcinoma, head and neck cancer, hematological cancer, and combinations thereof.

[0024] The present invention provides methods for treating and / or preventing cancer or for prolonging survival of a subject suffering from cancer. In some embodiments, the method comprises administering to the subject an effective amount of an antibody disclosed herein, an immunoconjugate disclosed herein, or a pharmaceutical composition disclosed herein. In some embodiments, the tumor exhibits high microsatellite instability (MSI). In some embodiments, the tumor is selected from mesothelioma, lung cancer, pancreatic cancer, ovarian cancer, breast cancer, colon cancer, pleural tumor, glioblastoma, esophageal cancer, gastric cancer, synovial sarcoma, thymic cancer, endometrial cancer, gastric tumor, cholangiocarcinoma, head and neck cancer, hematological cancer, and combinations thereof.

[0025] The present invention further provides any of the antibodies and / or pharmaceutical compositions disclosed herein for use as a medicament. The present invention further provides any of the antibodies and / or pharmaceutical compositions disclosed herein for treating cancer. In some embodiments, the tumor exhibits high microsatellite instability (MSI). In some embodiments, the cancer is selected from mesothelioma, lung cancer, pancreatic cancer, ovarian cancer, breast cancer, colon cancer, pleural tumor, glioblastoma, esophageal cancer, gastric cancer, synovial sarcoma, thymic cancer, endometrial cancer, gastric tumor, cholangiocarcinoma, head and neck cancer, hematological cancer, and combinations thereof.

[0026] The present invention provides a kit comprising an antibody disclosed herein, an immunoconjugate disclosed herein, a pharmaceutical composition disclosed herein, a nucleic acid disclosed herein, a vector disclosed herein, or an immunoresponsive cell disclosed herein. In some embodiments, the kit further comprises instructions for treating and / or preventing a neoplasm. [Brief description of the drawings]

[0027] [Figure 1A] A schematic diagram of an exemplary anti-OX40 bivalent antibody is shown. [Figure 1B] Binding of the c5E10 antibody to recombinant human or mouse OX40 ECD as assessed by ELISA is shown. [Figure 1C] We have depicted an engineering strategy for four human-mouse chimeric OX40 ECDs, each containing one human OX40 ECD in which one of the four cysteine-rich domains (CRDs) is replaced with the corresponding mouse OX40 CRD. [Figure 1D] Binding of different anti-OX40 antibodies to human OX40 ECD or human-mouse chimeric OX40 ECD as assessed by ELISA is shown. [Figure 2A]Whole cell binding of humanized anti-OX40 antibodies to human or cynomolgus OX40 assessed by flow cytometry is shown. Jurkat cells expressing human OX40 (Figure 2A), CHO cells expressing human OX40 (Figure 2B) and CHO cells expressing cynomolgus OX40 (Figure 2C) were incubated with the indicated anti-OX40 bivalent antibodies and stained with anti-human IgG Fc antibody conjugated to Alexa Fluor488. Fluorescence intensity was measured by flow cytometry. [Figure 2B] Whole cell binding of humanized anti-OX40 antibodies to human or cynomolgus OX40 assessed by flow cytometry is shown. Jurkat cells expressing human OX40 (Figure 2A), CHO cells expressing human OX40 (Figure 2B) and CHO cells expressing cynomolgus OX40 (Figure 2C) were incubated with the indicated anti-OX40 bivalent antibodies and stained with anti-human IgG Fc antibody conjugated to Alexa Fluor488. Fluorescence intensity was measured by flow cytometry. [Figure 2C] Whole cell binding of humanized anti-OX40 antibodies to human or cynomolgus OX40 assessed by flow cytometry is shown. Jurkat cells expressing human OX40 (Figure 2A), CHO cells expressing human OX40 (Figure 2B) and CHO cells expressing cynomolgus OX40 (Figure 2C) were incubated with the indicated anti-OX40 bivalent antibodies and stained with anti-human IgG Fc antibody conjugated to Alexa Fluor488. Fluorescence intensity was measured by flow cytometry. [Figure 3A] A schematic diagram of an exemplary anti-OX40 tetravalent antibody is shown. [Figure 3B] The binding affinities of 1B3 and 2B7 bivalent and tetravalent antibodies to recombinant human OX40-Fc as measured by Octet are shown. [Figure 4A]Whole cell binding of anti-OX40 antibodies to human and cynomolgus OX40 assessed by flow cytometry. Jurkat cells expressing human OX40 (Figure 4A), CHO cells expressing human OX40 (Figure 4B), parental OX40-negative CHO cells (Figure 4C), and CHO cells expressing cynomolgus OX40 (Figure 4D) were incubated with 1B3 and 2B7 bivalent and tetravalent antibodies, and binding of these antibodies to the cells was analyzed by flow cytometry. [Figure 4B] Whole cell binding of anti-OX40 antibodies to human and cynomolgus OX40 assessed by flow cytometry. Jurkat cells expressing human OX40 (Figure 4A), CHO cells expressing human OX40 (Figure 4B), parental OX40-negative CHO cells (Figure 4C), and CHO cells expressing cynomolgus OX40 (Figure 4D) were incubated with 1B3 and 2B7 bivalent and tetravalent antibodies, and binding of these antibodies to the cells was analyzed by flow cytometry. [Figure 4C] Whole cell binding of anti-OX40 antibodies to human and cynomolgus OX40 assessed by flow cytometry. Jurkat cells expressing human OX40 (Figure 4A), CHO cells expressing human OX40 (Figure 4B), parental OX40-negative CHO cells (Figure 4C), and CHO cells expressing cynomolgus OX40 (Figure 4D) were incubated with 1B3 and 2B7 bivalent and tetravalent antibodies, and binding of these antibodies to the cells was analyzed by flow cytometry. [Figure 4D] Whole cell binding of anti-OX40 antibodies to human and cynomolgus OX40 assessed by flow cytometry. Jurkat cells expressing human OX40 (Figure 4A), CHO cells expressing human OX40 (Figure 4B), parental OX40-negative CHO cells (Figure 4C), and CHO cells expressing cynomolgus OX40 (Figure 4D) were incubated with 1B3 and 2B7 bivalent and tetravalent antibodies, and binding of these antibodies to the cells was analyzed by flow cytometry. [Figure 5A]The effect of anti-OX40 antibodies on IL-2 release from human peripheral blood lymphocytes (PBMCs) stimulated with Staphylococcal enterotoxin B (SEB) was shown. Figure 5A shows that 1B3 and 2B7 tetravalent antibodies increased IL-2 secretion from SEB-stimulated PBMCs when co-cultured with human FcγRIIB / HEK293 cells. [Figure 5B] FIG. 5B shows that 1B3 and 2B7 tetravalent antibodies had no effect on IL-2 production in PBMCs stimulated with SEB in the absence of human FcγRIIB / HEK293 cells in the cell culture. [Figure 6A] We showed that anti-OX40 antibody induces the release of IL-2 and IFNγ from activated T cells. Human T cells were stimulated for 3 days with anti-CD3 beads and 2B7 tetravalent antibody or one of these two reference antibodies in the presence (Figures 6A and 6C) or absence (Figures 6B and 6D) of mitomycin C-treated FcγRIIB / HEK293 cells. IL-2 (Figures 6A and 6B) and IFNγ (Figures 6C and 6D) were assessed in cell culture supernatants by TR-FRET. [Figure 6B] We showed that anti-OX40 antibody induces the release of IL-2 and IFNγ from activated T cells. Human T cells were stimulated for 3 days with anti-CD3 beads and 2B7 tetravalent antibody or one of these two reference antibodies in the presence (Figures 6A and 6C) or absence (Figures 6B and 6D) of mitomycin C-treated FcγRIIB / HEK293 cells. IL-2 (Figures 6A and 6B) and IFNγ (Figures 6C and 6D) were assessed in cell culture supernatants by TR-FRET. [Figure 6C] We showed that anti-OX40 antibody induces the release of IL-2 and IFNγ from activated T cells. Human T cells were stimulated for 3 days with anti-CD3 beads and 2B7 tetravalent antibody or one of these two reference antibodies in the presence (Figures 6A and 6C) or absence (Figures 6B and 6D) of mitomycin C-treated FcγRIIB / HEK293 cells. IL-2 (Figures 6A and 6B) and IFNγ (Figures 6C and 6D) were assessed in cell culture supernatants by TR-FRET. [Figure 6D]We showed that anti-OX40 antibody induces the release of IL-2 and IFNγ from activated T cells. Human T cells were stimulated for 3 days with anti-CD3 beads and 2B7 tetravalent antibody or one of these two reference antibodies in the presence (Figures 6A and 6C) or absence (Figures 6B and 6D) of mitomycin C-treated FcγRIIB / HEK293 cells. IL-2 (Figures 6A and 6B) and IFNγ (Figures 6C and 6D) were assessed in cell culture supernatants by TR-FRET. [Figure 7A] We demonstrated the promotion of T cell proliferation by anti-OX40 antibody. Human T cells were stimulated for 5 days with anti-CD3 beads and 2B7 tetravalent antibody or one of these two reference antibodies in the presence (Figure 7A) or absence (Figure 7B) of mitomycin C-treated FcγRIIB / HEK293 cells. T cell proliferation was measured by adding 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazole (MTS) and measuring OD490 after color development. Human T cells were labeled with 2.5 μM carboxyfluorescein isosuccinimide ester (CFSE) and stimulated for 5 days with anti-CD3 beads and 2B7 tetravalent antibody or one of these two reference antibodies using mitomycin C-treated FcγRIIB / HEK293 cells. T cell proliferation was monitored by flow cytometry (FIG. 7C), and the relationship between T cell proliferation and the concentration of anti-OX40 antibody was plotted (FIG. 7D). [Figure 7B]We demonstrated the promotion of T cell proliferation by anti-OX40 antibody. Human T cells were stimulated for 5 days with anti-CD3 beads and 2B7 tetravalent antibody or one of these two reference antibodies in the presence (Figure 7A) or absence (Figure 7B) of mitomycin C-treated FcγRIIB / HEK293 cells. T cell proliferation was measured by adding 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazole (MTS) and measuring OD490 after color development. Human T cells were labeled with 2.5 μM carboxyfluorescein isosuccinimide ester (CFSE) and stimulated for 5 days with anti-CD3 beads and 2B7 tetravalent antibody or one of these two reference antibodies using mitomycin C-treated FcγRIIB / HEK293 cells. T cell proliferation was monitored by flow cytometry (FIG. 7C), and the relationship between T cell proliferation and the concentration of anti-OX40 antibody was plotted (FIG. 7D). [Figure 7C] We demonstrated the promotion of T cell proliferation by anti-OX40 antibody. Human T cells were stimulated for 5 days with anti-CD3 beads and 2B7 tetravalent antibody or one of these two reference antibodies in the presence (Figure 7A) or absence (Figure 7B) of mitomycin C-treated FcγRIIB / HEK293 cells. T cell proliferation was measured by adding 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazole (MTS) and measuring OD490 after color development. Human T cells were labeled with 2.5 μM carboxyfluorescein isosuccinimide ester (CFSE) and stimulated for 5 days with anti-CD3 beads and 2B7 tetravalent antibody or one of these two reference antibodies using mitomycin C-treated FcγRIIB / HEK293 cells. T cell proliferation was monitored by flow cytometry (FIG. 7C), and the relationship between T cell proliferation and the concentration of anti-OX40 antibody was plotted (FIG. 7D). [Figure 7D]We demonstrated the promotion of T cell proliferation by anti-OX40 antibody. Human T cells were stimulated for 5 days with anti-CD3 beads and 2B7 tetravalent antibody or one of these two reference antibodies in the presence (Figure 7A) or absence (Figure 7B) of mitomycin C-treated FcγRIIB / HEK293 cells. T cell proliferation was measured by adding 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazole (MTS) and measuring OD490 after color development. Human T cells were labeled with 2.5 μM carboxyfluorescein isosuccinimide ester (CFSE) and stimulated for 5 days with anti-CD3 beads and 2B7 tetravalent antibody or one of these two reference antibodies using mitomycin C-treated FcγRIIB / HEK293 cells. T cell proliferation was monitored by flow cytometry (FIG. 7C), and the relationship between T cell proliferation and the concentration of anti-OX40 antibody was plotted (FIG. 7D). [Figure 8A] The in vivo efficacy of anti-OX40 antibodies was demonstrated in a human OX40 knock-in C57BL / 6 mouse MC38 colon tumor model. Each mouse was subcutaneously inoculated with 0.5x106 MC38 tumor cells. When tumor size reached approximately 60mm3, mice received the indicated doses of anti-OX40 antibodies twice a week for three weeks. Figure 8A shows dose-dependent inhibition of tumor growth by 2B7 tetravalent antibody. The Y axis represents the average tumor size of eight mice in each group, and the X axis represents the number of days after treatment. [Figure 8B] FIG. 8B shows the average body weight of each group of mice during the treatment period. [Figure 8C] Figure 8C shows the tumor growth curve in mice treated with anti-OX40 antibody. Mice were treated with 3 mg / kg of 2B7 tetravalent antibody or reference antibody twice a week for 3 weeks. 2B7 was more effective than the reference antibody. [Figure 8D] FIG. 8D shows the change in individual tumor volume over time in each treatment group from FIG. 8C. [Figure 9A]The in vivo efficacy of anti-OX40 antibodies was demonstrated in a CT26 colon cancer model in human OX40 knock-in BALB / c mice. Mice were subcutaneously injected with 0.5x106 CT26 tumor cells. When tumor size reached approximately 65mm3, mice were intraperitoneally treated with the indicated doses of 2B7 tetravalent antibody or reference antibody 2 twice a week for 3 weeks. Figure 9A shows the tumor growth curves of mice treated with anti-OX40 antibodies. [Figure 9B] FIG. 9B shows the tumor growth curves for each mouse in each treatment group in FIG. 9A. [Figure 9C] FIG. 9C shows the mean body weight in each treatment group. [Figure 10A] The in vivo efficacy of anti-OX40 antibodies was demonstrated in a human OX40 knock-in C57BL / 6 mouse Pan02 pancreatic tumor model. Mice were subcutaneously injected with 3x106 Pan02 tumor cells. When the average tumor size reached 92.6mm3, mice were randomly grouped, 10 mice per group, and received the indicated treatments. Figure 10A shows a comparison of 2B7 with the reference 2 antibody. [Figure 10B] Figure 10B shows a comparison between monotherapy with 2B7 and combination therapy with each anti-PD1 antibody (RMP1-14). [Figure 10C] FIG. 10C shows the average body weight of mice in each treatment group. [Figure 10D] FIG. 10D shows the change in individual tumor volume over time in each treatment group. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0028] The present invention provides isolated monoclonal antibodies and antibody derivatives that specifically bind to OX40 with high affinity, including monospecific anti-OX40 antibodies and multispecific antibodies that bind to OX40 and one or more additional targets. In some embodiments, the antibodies or antibody derivatives disclosed herein include single domain antibodies that bind to OX40. The present invention further provides methods for preparing and using the antibodies and antibody derivatives disclosed herein and pharmaceutical compositions that include these antibodies and antibody derivatives, for use in treating diseases and disorders such as cancer. The present invention is based in part on the discovery of novel single domain antibodies that bind to OX40, which can provide improved anti-tumor effects by targeting tumor cells and / or increasing the immune response against tumor cells.

[0029] For purposes of clarity, and not limitation, specific embodiments of the presently disclosed subject matter are set forth as follows.

[0030] 1. Definition, 2. Antibodies and antibody derivatives, 3. How to use; 4. Drug Formulations, and 5. Products.

[0031] 1.Definition The term "antibody" as used herein includes full length antibodies and any antigen-binding fragments thereof (i.e., antibody fragments). An "antibody" may be a separate molecule or part of an antibody derivative. Exemplary antibody derivatives include, but are not limited to, multispecific antibodies (e.g., bispecific antibodies), antigen-recognizing receptors (e.g., chimeric antigen receptors), antibody conjugates that include additional protein or non-protein moieties (e.g., antibody-drug conjugates or antibodies coated with polymers), and other multifunctional molecules that include antibodies.

[0032] "Full-length antibody", "complete antibody" and "whole antibody" refer to an antibody having a heavy chain comprising an Fc region similar to the structure of a natural antibody or as defined herein. In some embodiments, a full-length antibody comprises two heavy chains and two light chains. In some embodiments, the variable regions of the light and heavy chains are responsible for antigen binding. The variable regions of the heavy and light chains may be referred to as "VH" and "VL", respectively. The variable regions in the two chains usually comprise three highly variable loops, which are referred to as complementarity determining regions (CDRs) (light chain (LC) CDR comprising LC-CDR1, LC-CDR2 and LC-CDR3, heavy chain (HC) CDR comprising HC-CDR1, HC-CDR2 and HC-CDR3). The CDR boundaries of the antibodies and antigen-binding fragments disclosed herein may be defined or identified by well-known conventions, such as those of Kabat, Chothia, MacCallum, IMGT and AHo, as described below. The three CDRs of the heavy or light chain are inserted between flanking segments called framework regions (FRs), which are more conserved than the CDRs, and form a scaffold supporting the hypervariable loops. The constant regions of the heavy and light chains are not involved in antigen binding, but exhibit multiple effector functions. Antibodies are classified based on the amino acid sequence of the antibody heavy chain constant region. The five major classes or isotypes of antibodies are IgA, IgD, IgE, IgG, and IgM, which are characterized by the presence of α, δ, ε, γ, and μ heavy chains, respectively. Some of the major antibody classes are divided into subclasses, e.g., IgG1 (γ1 heavy chain), IgG2 (γ2 heavy chain), IgG3 (γ3 heavy chain), IgG4 (γ4 heavy chain), IgA1 (α1 heavy chain), or IgA2 (α2 heavy chain). In some embodiments, the full-length antibody is glycosylated. In some embodiments, the full-length antibody comprises glycans linked to its Fc region. In some embodiments, a full-length antibody comprises branched glycans.

[0033] As used herein, the terms "antigen-binding portion," "antibody fragment," and "antibody portion" of an antibody refer to one or more fragments of an antibody that retain the ability to specifically bind to an antigen. It has been shown that the antigen-binding function of an antibody can be performed by fragments of a full-length antibody. Examples of antibody fragments include, but are not limited to, Fv, Fab, Fab', Fab'-SH, F(ab')2, diabodies, linear antibodies, single-chain antibody molecules (e.g., scFv and scFv-Fc), single domain antibodies, VHH, VHH-Fc, nanobodies, domain antibodies, bivalent domain antibodies, or any other fragment of an antibody that binds to an antigen, or combinations thereof. "VHH" refers to a single domain antibody isolated from a camelid. In some embodiments, the VHH comprises the heavy chain variable region of a camelid heavy chain antibody. In some embodiments, the size of the VHH does not exceed about 25 kDa. In some embodiments, the size of the VHH does not exceed about 20 kDa. In some embodiments, the size of the VHH does not exceed about 15 kDa.

[0034] An "antibody that cross-competes for binding" with a reference antibody refers to an antibody that blocks the binding of the reference antibody to its antigen by 50% or more in a competitive assay, and conversely, the reference antibody blocks the binding of the antibody to its antigen by 50% or more in a competitive assay. Exemplary competitive assays are described in Antibodies, Harlow and Lane (Cold Spring Harbor Press, Cold Spring Harbor, NY).

[0035] "Fv" is the smallest antibody fragment, which contains a complete antigen recognition and binding site. The fragment consists of a dimer of one heavy and one light chain variable domain in tight, non-covalent association. Folding of the two domains releases six hypervariable loops (three loops each in the heavy and light chains), which provide the amino acid residues for antigen binding and confer the binding specificity of the antibody to the antigen. However, even a single variable domain (or even a half Fv containing only the three CDRs with specificity for the antigen) can recognize and bind the antigen, although it may do so with a lower affinity than the complete binding site.

[0036] "Single-chain Fv", also abbreviated as "sFv" or "scFv", is a VF linked to a single polypeptide chain. H and V L In some embodiments, the scFv polypeptide is an antibody fragment comprising the V H and V L The scFv further comprises a polypeptide linker between the domains that enables the scFv to form the desired structure for antigen binding. For a general description of scFvs, see Pluckthun, The Pharmacology of Monoclonal Antibodies, vol. 113, Rosenburg and Moore eds., Springer-Verlag, New York, pp. 269-315 (1994).

[0037] For the purposes of this specification, a "receptor human framework" or a "human framework" is a framework that comprises the amino acid sequence of a light chain variable domain (VL) framework or a heavy chain variable domain (VH) framework derived from a human immunoglobulin framework or a human shared framework. A recipient human framework that is "derived" from a human immunoglobulin framework or a human shared framework may comprise the same amino acid sequence or may comprise amino acid sequence changes. In some embodiments, the number of amino acid changes is 10 or less, 9 or less, 8 or less, 7 or less, 6 or less, 5 or less, 4 or less, 3 or less, or 2 or less. In some embodiments, the VL recipient human framework and the VL human immunoglobulin framework sequence or the human shared framework sequence are identical in terms of sequence.

[0038] "Affinity" refers to the strength of the sum of non-covalent interactions between a single binding site of a molecule (e.g., an antibody) and its binding partner (e.g., an antigen). Unless otherwise specified, as used herein, "binding affinity" refers to internal binding affinity, which reflects a 1:1 interaction between members of a binding pair (e.g., an antibody and an antigen). The affinity of a molecule X for a partner Y may be typically expressed as a dissociation constant (KD). Affinity may be measured by conventional methods known in the art, including those described herein. Specific illustrations and exemplary embodiments for measuring binding affinity are described below.

[0039] An "affinity matured" antibody refers to an antibody that has one or more modifications in one or more CDRs or hypervariable regions (HVRs) compared to a parent antibody that does not possess such modifications, which modifications provide an improved affinity of the antibody for antigen.

[0040] The terms "anti-OX40 antibody" and "antibody that binds to OX40" refer to an antibody that can bind to OX40 with sufficient affinity such that it can be used as a diagnostic and / or therapeutic agent targeting OX40. In one embodiment, the extent of binding of an anti-OX40 antibody to an unrelated, non-OX40 protein is less than about 10% of the binding of the antibody to OX40, as measured, for example, by a surface plasmon resonance assay. In some embodiments, an antibody that binds to OX40 has an affinity of < about 1 μM, < about 100 nM, < about 10 nM, < about 1 nM, < about 0.1 nM, < about 0.01 nM, or < about 0.001 nM (e.g., 10 -8 M or smaller, e.g., 10 -8 M to 10 -12 M, for example 10 -9 M to 10 -10 The anti-OX40 antibody has a dissociation constant (KD) of 0.01 M. In some embodiments, the anti-OX40 antibody binds to an OX40 epitope that is conserved in OX40 from different species. In some embodiments, the anti-OX40 antibody binds to an epitope on OX40 in the ECD of the protein. In some embodiments, the anti-OX40 antibody binds to an epitope on OX40 in domain 2 (CRD2) of the protein.

[0041] The term "chimeric" antibody refers to an antibody in which a portion of the heavy and / or light chain is derived from a particular source or species, while the remaining portions of the heavy and / or light chain are derived from a different source or species. In some embodiments, a chimeric antibody disclosed herein comprises a murine heavy chain variable region and a human Fc region. In some embodiments, a chimeric antibody disclosed herein comprises a camelid heavy chain variable region and a human Fc region.

[0042] As used herein, the term "CDR" or "complementarity determining region" refers to discontinuous antigen-binding sites within the variable regions of the heavy and / or light chains. These specific regions have been described in Kabat et al., J. Biol. Chem. 252: 6609-6616 (1977), Kabat et al., US Dept. of Health and Human Services, “Sequences of proteins of immunological interest” (1991), Chothia et al., J. Mol. Biol. 196: 901-917 (1987), Al-Lazikani B. et al.,J. Mol. Biol.,273: 927-948 (1997), MacCallum et al.,J. Mol. Biol. 262: 732-745 (1996), Abhinandan and Martin,Mol. Immunol.,45: 3832-3839 (2008), Lefranc MP et al.,Dev. Comp. Immunol.,27: 55-77 (2003), and Honegger and Pluckthun, J. Mol. Biol.,309: 657-670 (2001), where the definitions include overlapping or subsets of amino acid residues when compared to one another. However, it is intended that the application of any one definition to refer to an antibody or grafted antibody or variant thereof is within the scope of the term as defined and used herein. The amino acid residues covering the CDRs defined in each of the above references are included in Table 1 below for comparison. CDR prediction algorithms and interfaces are known in the art and include, for example, Abhinandan and Martin, Mol. Immunol., 45: 3832-3839 (2008), Ehrenmann F. et al., Nucleic Acids Res., 38: D301-D307 (2010), and Adolf-Bryfogle J. et al., Nucleic Acids Res., 43: D432-D438 (2015).The contents of the references cited in this section are incorporated herein by reference in their entirety, may be used in this application, and may be included in one or more claims herein.

[0043] [Table 1] 1 Residue numbering follows the nomenclature of Kabat et al. (ibid.). 2 Residue numbering follows the nomenclature of Chothia et al. (ibid.). 3 Residue numbering follows the nomenclature of MacCallum et al. (ibid.). 4 Residue numbering follows the nomenclature of Lefranc et al. (ibid.). 5 Residue numbering follows the nomenclature of Honegger and Pluckthun (ibid.).

[0044] The phrases "variable region residue numbering, e.g., according to Kabat" or "amino acid position numbering, e.g., according to Kabat" and variations thereof refer to the numbering system used for the heavy or light chain variable regions of the antibody assembler of Kabat et al., supra. Using this numbering system, the actual linear amino acid sequence may contain fewer or additional amino acids corresponding to a shortening or insertion of a FR or CDR of the variable region. For example, a heavy chain variable region may contain a single amino acid insertion after residue 52 of H2 (residue 52a according to Kabat) and inserted residues after heavy chain FR residue 82 (e.g., residues 82a, 82b, and 82c, etc., according to Kabat). The Kabat numbering of residues of a given antibody can be determined by alignment of the antibody sequence with the "standard" Kabat numbering sequence at the regions of homology.

[0045] In some embodiments, the amino acid residues covering the CDRs of a single domain antibody are defined according to the IMGT nomenclature of Lefranc et al., supra. In some embodiments, the amino acid residues covering the CDRs of a full length antibody are defined according to the Kabat nomenclature of Kabat et al., supra. In some embodiments, the residue numbering in an immunoglobulin heavy chain, e.g., an Fc region, is that of the EU index as set forth in Kabat et al., supra. The "EU index as set forth in Kabat" is the residue numbering of the human IgG1 EU antibody.

[0046] "Framework" or "FR" refers to those variable domain residues other than the CDR residues as herein defined.

[0047] A "humanized" antibody refers to a chimeric antibody that comprises amino acid residues from non-human CDRs / HVRs and amino acid residues from human FRs. In some embodiments, a humanized antibody comprises at least one, and typically two, variable domains, in which all or essentially all of the HVRs / CDRs correspond to those of a non-human antibody and all or essentially all of the FRs correspond to those of a human antibody. A humanized antibody may optionally comprise at least a portion of an antibody constant region derived from a human antibody. A "humanized form" of an antibody (e.g., a non-human antibody) refers to an antibody that has been humanized.

[0048] A "human antibody" is an antibody having an amino acid sequence that corresponds to that of an antibody produced by a human and / or that has been prepared by any of the techniques disclosed herein for the preparation of human antibodies. This definition of a human antibody specifically excludes humanized antibodies that contain non-human antigen-binding residues. Human antibodies may be produced using a variety of techniques known in the art, including phage display libraries. Hoogenboom and Winter, J. Mol. Biol., 227:381 (1991); Marks et al., J. Mol. Biol., 222:581 (1991). Also usable for the preparation of human monoclonal antibodies are the methods described in Cole et al., Monoclonal Antibodies and Cancer Therapy, Alan R. Liss, p. 77 (1985); Boerner et al., J. Immunol., 147(1):86-95 (1991). See also van Dijk and van de Winkel, Curr. Opin. Pharmacol., 5: 368-74 (2001). Human antibodies can be prepared by administering antigen to transgenic animals, e.g., immunized xenograft mice (xenotransplanted mice), which have been modified to produce such antibodies in response to antigenic challenge but have had their endogenous loci disabled (e.g., XENOMOUSE TM (See U.S. Patent Nos. 6,075,181 and 6,150,584, which relate to human antibodies produced by human B cell hybridoma technology.) Also see, e.g., Li et al., Proc. Natl. Acad. Sci. USA, 103:3557-3562 (2006), which relates to human antibodies produced by human B cell hybridoma technology.

[0049] "Percent amino acid sequence identity (%)" or "homology" between the polypeptide and antibody sequences identified herein is defined as the percentage of amino acid residues in a candidate sequence that are the same as those in the polypeptide being compared, after alignment of the sequences (taking into account any conservative substitutions as part of sequence identity). For the purpose of determining percent amino acid sequence identity, alignment can be achieved in a variety of ways in the art, for example, using publicly available computer software, such as BLAST, BLAST-2, ALIGN, Megalign (DNASTAR) or MUSCLE software. Those skilled in the art can determine suitable parameters to be used for measuring alignment, including any algorithm that achieves maximum alignment within the full length of the sequences being compared. However, for the purposes of this specification, the sequence comparison computer program MUSCLE is used to generate values ​​for percent amino acid sequence identity (Edgar, RC, Nucleic Acids Research 32(5):1792-1797, 2004; Edgar, RC, BMC Bioinformatics 5(1):113, 2004).

[0050] "Homology" refers to sequence similarity or sequence identity between two polypeptides or two nucleic acid molecules. When a position in two compared sequences is occupied by the same base or amino acid monomer subunit, for example, when a position in each of two DNA molecules is occupied by adenine, the molecules are homologous at this position. The percentage of homology between two sequences is a function of the number of matching or homologous positions shared by the two sequences divided by the number of positions compared, multiplied by 100. For example, if 6 out of 10 positions in two sequences are matching or homologous, the two sequences are 60% homologous. For example, the DNA sequences ATTGCC and TATGGC have 50% homology. Usually, the comparison is performed when the two sequences are aligned to give maximum homology.

[0051] The "light chains" of any mammalian species of antibody (e.g., immunoglobulin) can be assigned to one of two clearly distinct types, called kappa ("κ") and lambda ("λ"), depending on the amino acid sequence of their constant domain.

[0052] The term "constant domain" refers to a portion of an immunoglobulin molecule that has a more conserved amino acid sequence relative to another portion of the immunoglobulin, the variable domain, and which contains the antigen-binding site. The constant domain is composed of the C H 1. C H 2 and C H 3 Domain (C H ) and light chain C L The domain.

[0053] In some embodiments, the "CH1 domain" (also called "C1" for "H1" domain) typically extends from about amino acid 118 to about amino acid 215 (EU numbering system).

[0054] In some embodiments, the "hinge region" is generally defined in IgG as the region corresponding to Glu216 to Pro230 of human IgG1 (Burton, Molec. Immunol. 22:161-206 (1985)). Hinge regions of other IgG isotypes can be aligned with the IgG1 sequence by placing the first and last cysteine ​​residues that form S-S bonds between the heavy chains in the same positions.

[0055] In some embodiments, the "CH2 domain" of a human IgG Fc region (also called the "C2" domain) typically extends from about amino acid 231 to about amino acid 340. The CH2 domain is unique because it is not tightly paired with another domain. Instead, two N-linked branched carbohydrate chains are inserted between the two CH2 domains of an intact native IgG molecule. Speculation has it that the carbohydrates provide an alternative to domain-domain pairing and may contribute to stabilization of the CH2 domain. Burton, Molec Immunol. 22:161-206 (1985).

[0056] In some embodiments, the "CH3 domain" (also referred to as the "C2" domain) comprises residues between the CH2 domain and the C-terminus of the Fc region (i.e., from about amino acid residue 341 to the C-terminus of the antibody sequence), usually at amino acid residue 446 or 447 of IgG.

[0057] "Fc region" or "fragment crystallizable region" herein is intended to define the C-terminal region of an immunoglobulin heavy chain, and includes native sequence Fc regions and variant Fc regions, or dimers thereof. In some embodiments, a human IgG Fc region extends from Cys226 to its carboxyl terminus. In some embodiments, a human IgG Fc region extends from Pro231 to its carboxyl terminus. In some embodiments, a human IgG Fc region includes a CH2 domain and a CH3 domain. In some embodiments, the C-terminal lysine of the Fc region (residue 447 according to the European Union numbering system) can be removed, for example, during production or purification of the antibody, or by recombinantly engineering the nucleic acid encoding the antibody heavy chain. In some embodiments, a composition of complete antibodies can include an antibody population with all K447 residues removed, an antibody population in which the K447 residue has not been removed, or a mixture of antibodies with or without the K447 residue. Native sequence Fc regions applicable to the antibodies described herein include human IgG1, IgG2 (IgG2A, IgG2B), IgG3 and IgG4 Fc regions.

[0058] "Fc receptor" or "FcR" describes a receptor that binds to the Fc region of an antibody. A preferred FcR is a native human FcR. In addition, a preferred FcR is one that binds an IgG antibody (gamma receptor) and includes receptors of the FcγRI, FcγRII and FcγRIII subclasses, including allelic variants and splice forms of these receptors. FcγRII receptors include FcγRIIA ("activating receptors") and FcγRIIB ("inhibiting receptors"), which have similar amino acid sequences and the primary distinction is in their cytoplasmic domains. Activating receptor FcγRIIA contains an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. Inhibiting receptor FcγRIIB contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic domain. (See M. Daeron, Annu. Rev. Immunol. 15:203-234 (1997). Overviews of FcRs are provided in Ravetch and Kinet, Annu. Rev. Immunol. 9: 457-92 (1991), Capel et al., Immunomethods 4: 25-34 (1994), and de Haas et al., J. Lab. Clin. Med. 126: 330-41 (1995). The term "FcR" herein is intended to cover other FcRs and includes FcRs identified in the future.

[0059] The term "epitope" as used herein refers to the specific atom or amino acid group on an antigen to which an antibody or antibody derivative binds. Two antibodies or antigen-binding portions can bind to the same epitope in an antigen if they have competitive binding to the antigen.

[0060] As used herein, the terms "specifically binds," "specifically recognizes," and "has specificity for" refer to a measurable and reproducible interaction, such as the binding of a target to an antibody or antibody portion, which determines the presence of the target in the presence of a heterogeneous (including biomolecule) population. For example, an antibody or antibody portion that specifically recognizes a target (which may be an epitope) is an antibody or antibody portion that binds to the target with a longer affinity, avidity, readiness, and / or duration than binding to other targets. In some embodiments, the extent of binding of the antibody to an unrelated target is about 10% less than the extent of binding of the antibody to the target, as measured, for example, by radioimmunoassay (RIA). In some embodiments, the dissociation constant (K D )≦10 -5 M, ≦10 -6 M, ≦10 -7 M, ≦10 -8 M, ≦10 -9 M, ≦10 -10 M, ≦10 -11 M or ≦10 -12 In some embodiments, the antibody specifically binds to an epitope of a protein that is conserved among proteins from different species. In some embodiments, specific binding may include, but is not limited to, exclusive binding. The binding specificity of an antibody or antigen-binding domain may be determined experimentally by methods known in the art. Such methods include Western blot, ELISA-, RIA-, ECL-, IRMA-, EIA-, BIACORE. TM These include, but are not limited to, assays and peptide scanning.

[0061] An "isolated" antibody (or construct) is an antibody that has been identified, isolated and / or recovered from a component of its production environment (e.g., natural or recombinant). In some embodiments, an isolated polypeptide is free or essentially free from association with all other components in the production environment.

[0062] An "isolated" nucleic acid molecule encoding a construct, antibody or antigen-binding fragment thereof described herein is a nucleic acid molecule that has been identified and isolated from at least one contaminant nucleic acid molecule that is normally associated with it in its production environment. In some embodiments, an isolated nucleic acid is free or essentially free from association with all components associated with the production environment. The format of the isolated nucleic acid molecule encoding the polypeptides and antibodies described herein is different from the naturally occurring format or background. Thus, an isolated nucleic acid molecule is different from the nucleic acid encoding the polypeptides and antibodies described herein that is naturally present in a cell. Isolated nucleic acid includes a nucleic acid molecule contained in a cell that normally contains the nucleic acid molecule, but the nucleic acid molecule is present extrachromosomally or in a chromosomal location that is different from its natural chromosomal location.

[0063] The term "control sequences" refers to DNA sequences necessary for the expression of an operably linked coding sequence in a particular host organism. For example, control sequences applicable to prokaryotes include promoters, optional operon sequences, and ribosome binding sites. Eukaryotic cells are known to utilize promoters, polyadenylation signals, and enhancers.

[0064] A nucleic acid is "operably linked" when it is in a functional relationship with another nucleic acid sequence. For example, a presequence or secretory leader sequence DNA is operably linked to a polypeptide DNA if it is expressed as a preprotein involved in the secretion of the polypeptide, a promoter or enhancer is operably linked to a coding sequence if it affects the transcription of the sequence, or a ribosome binding site is operably linked to a coding sequence if it is positioned to facilitate translation. Usually, "operably linked" means that the DNA sequences being linked are contiguous, and in the case of a secretory leader sequence, contiguous and in reading frame. Enhancers, however, do not necessarily have to be contiguous. Linking is accomplished by convenient restriction sites. If no such sites exist, synthetic oligonucleotide adaptors or linkers are used in accordance with conventional practice.

[0065] As used herein, the term "vector" refers to a nucleic acid molecule capable of propagating another nucleic acid to which it is linked. The term includes vectors that are self-replicating nucleic acid structures and vectors that integrate into the genome of a host cell into which they are introduced. Some vectors are capable of directing the expression of a nucleic acid to which they are operably linked. Such vectors are referred to herein as "expression vectors."

[0066] As used herein, the terms "transfected" or "transformed" or "transduced" refer to the process of transferring or introducing exogenous nucleic acid into a host cell. A "transfected" or "transformed" or "transduced" cell is a cell that has been transfected, transformed or transduced with exogenous nucleic acid, including the primary subject cell and its progeny.

[0067] The terms "host cell," "host cell line," and "host cell culture" are used interchangeably and refer to cells into which exogenous nucleic acid has been introduced, including the progeny of such cells. Host cells include "transformants" and "transformed cells," which include the primary transformed cell and its progeny, regardless of the number of passages. The nucleic acid content of the progeny may not be completely identical to the parent cell and may contain mutations. Included herein are mutant progeny that have the same function or biological activity as screened or selected for in the originally transformed cell.

[0068] The terms "subject," "individual," and "patient" are used interchangeably herein and refer to a mammal, including, but not limited to, a human, bovine, equine, feline, canine, rodent, or primate. In some embodiments, the subject is a human.

[0069] An "effective amount" of an agent refers to an amount that effectively achieves a desired therapeutic or prophylactic effect, at a required dosage and for a period of time. The particular dosage may vary depending on one or more of the particular agent selected, the subsequent administration regimen (whether or not combined with other compounds), the time of administration, the tissue imaged, and the physical delivery system associated therewith.

[0070] A "therapeutically effective amount" of a substance / molecule, agonist or antagonist of the present application may vary depending on factors such as, for example, the disease state, age, sex and weight of the individual, and the ability of the substance / molecule, agonist or antagonist to elicit a desired response in the individual. A therapeutically effective amount is further an amount in which any toxic or adverse effects of the substance / molecule, agonist or antagonist are countered by the therapeutically beneficial effects. A therapeutically effective amount can be delivered in one or more administrations.

[0071] A "prophylactically effective amount" refers to an amount effective, at dosages and for periods of time necessary, to achieve the desired prophylactic result. Typically, but not necessarily, since a prophylactic dose is used in subjects prior to or at an earlier stage of disease, such a prophylactically effective amount will be less than the therapeutically effective amount.

[0072] As used herein, "treatment" or "treating" is a method for obtaining a beneficial or desired result (including a clinical result). For purposes of this application, beneficial or desired clinical results include, but are not limited to, one or more of the following: alleviating one or more symptoms caused by a disease, reducing the extent of the disease, stabilizing the disease (e.g., preventing or slowing the progression of the disease), preventing or slowing the spread of the disease (e.g., metastasis), preventing or slowing the recurrence of the disease, slowing or slowing the progression of the disease, improving the disease state, providing relief (partial or complete) of the disease, reducing the dose of one or more other drugs required to treat the disease, slowing the progression of the disease, increasing or improving quality of life, increasing weight, and / or prolonging survival. "Treatment" further covers reducing the pathological consequences of cancer (e.g., tumor volume). The methods of this application contemplate any one or more of these aspects of treatment. "Treatment" does not necessarily mean that the disease being treated is cured.

[0073] It should be understood that embodiments of the present application described herein include "consisting of an embodiment" and / or "consisting essentially of an embodiment."

[0074] As used herein, the term "about" or "approximately" means that a particular value as determined by one of ordinary skill in the art is within an acceptable error range, which depends in part on how the value is measured or determined, i.e., limited by the measurement system. In some embodiments, "about" may mean within three or more than three standard differences, in accordance with the practice in the art. In some embodiments, "about" may refer to a range of at most 20% (e.g., at most 10%, at most 5%, or at most 1%) of a given value. In some embodiments, particularly with respect to biological systems and methods, the term may mean within an order of magnitude, e.g., within 5-fold or within 2-fold, of a value.

[0075] As used herein, the term "modulation" refers to a change in a positive or negative direction. Exemplary modulations include changes of about 1%, about 2%, about 5%, about 10%, about 25%, about 50%, about 75%, or about 100%.

[0076] As used herein, the term "increase" refers to a change in the positive direction of at least about 5%. The change may be about 5%, about 10%, about 25%, about 30%, about 50%, about 75%, about 100% or more.

[0077] As used herein, the term "reduction" refers to a change in the negative direction of at least about 5%. The change may be about 5%, about 10%, about 25%, about 30%, about 50%, about 75% or even about 100%.

[0078] The term "about X to Y" used in this specification has the same meaning as "about X to about Y."

[0079] As used in this specification and the appended claims, the singular forms "a," "or," and "the" include plural referents unless the context clearly dictates otherwise.

[0080] "Effector functions" refer to those biological activities of the Fc region of an antibody, which vary depending on the antibody isotype. Examples of antibody effector functions include C1q binding and complement-dependent cytotoxicity (CDC), Fc receptor binding, antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, down-regulation of cell surface receptors (e.g., B cell receptors), and B cell activation.

[0081] "Immunoconjugate" refers to an antibody conjugated to one or more heterologous molecules (including, but not limited to, cytotoxic agents).

[0082] The term "drug formulation" refers to a formulation that is in a form that allows the biologically active form of the active ingredient contained therein to be effective, and that does not contain other ingredients that are unacceptably toxic to the subject to which the formulation is administered.

[0083] As used herein, a "pharmaceutically acceptable carrier agent" refers to an ingredient in a drug formulation that is non-toxic to a subject, other than an active ingredient. Pharmaceutically acceptable carrier agents include, but are not limited to, buffers, excipients, stabilizers, or preservatives.

[0084] The term "variable region" or "variable domain" refers to the domain of an antibody heavy or light chain, which is involved in binding the antibody to an antigen. In some embodiments, the heavy and light chain variable domains of a natural antibody (VH and VL, respectively) usually have a similar structure, with each domain containing four conserved framework regions (FR) and three CDRs. (See, for example, Kindt et al. Kuby Immunology, 61 ed., WH Freeman and Co., page 91 (2007)). A single VH or VL domain is sufficient to confer antigen-binding specificity. It should be noted that a VH or VL domain can be used to screen a library of complementary VL or VH domains, respectively, to isolate specific antigen-binding antibodies from antigen-binding antibodies. See, e.g., Portolano et al., J. Immunol. 150:880-887 (1993); Clarkson et al., Nature 352:624-628 (1991).

[0085] As used herein, the term "antigen-recognizing receptor" refers to a receptor that can activate an immunoresponsive cell (e.g., a T cell) in response to its binding to an antigen. Non-limiting examples of antigen-recognizing receptors include natural and modified T cell receptors ("TCRs") and chimeric antigen receptors ("CARs").

[0086] As used herein, the term "chimeric antigen receptor" or "CAR" refers to a molecule that comprises an extracellular antigen-binding domain and a transmembrane domain fused to an intracellular signaling domain that can activate or stimulate an immunoresponsive cell. In some embodiments, the extracellular antigen-binding domain of the CAR comprises an antibody or an antibody fragment, such as a VHH or scFv. In some embodiments, an antibody (e.g., a VHH or scFv) is fused to a transmembrane domain, which is fused to an intracellular signaling domain. In some embodiments, a CAR is selected that has a high binding affinity or avidity for an antigen.

[0087] "Immunoresponsive cell" refers to a cell that functions in the immune response, or an ancestor or progeny thereof.

[0088] "OX40", "OX40 protein" or "OX40 polypeptide" refers to any OX40 polypeptide from any vertebrate (including mammals, e.g., primates (e.g., humans and cynomolgus monkeys)), or any fragment thereof, and may optionally contain at most one, at most two, at most three, at most four, at most five, at most six, at most seven, at most eight, at most nine, or at most ten amino acid substitutions, additions, and / or deletions. The term includes full-length, unprocessed OX40 and any form of OX40 produced by processing in a cell. The term further includes naturally occurring OX40 variants, e.g., splice variants or allelic variants. In some embodiments, the OX40 polypeptide comprises or has an amino acid sequence having at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, or at least about 100% homology or identity to the sequence having the following NCBI reference numbers: NP_003318.1, XP_016857721.1, XP_011540378.1, XP_016857720.1, XP_011540377.1, XP_011540376.1, or XP_011540379.1 (homology herein may be determined using standard software, such as BLAST or FASTA). In some embodiments, the amino acid sequence that the OX40 polypeptide comprises or has is the entire or continuous portion of SEQ ID NO:93.

[0089] The term "ECD of OX40" refers to the extracellular domain of OX40. In some embodiments, the ECD is domain 2 (CRD2) of the ECD of OX40. In some embodiments, the ECD is domain 4 (CRD4) of the ECD of OX40. In some embodiments, an exemplary OX40 polypeptide may comprise the amino acid sequence set forth in SEQ ID NO:94. In some embodiments, domain 2 (CRD2) of the ECD of an exemplary OX40 polypeptide may comprise the amino acid sequence set forth in SEQ ID NO:95. In some embodiments, domain 4 (CRD4) of the ECD of an exemplary OX40 polypeptide may comprise the amino acid sequence set forth in SEQ ID NO:96.

[0090] 2. Antibodies and antibody derivatives The present invention provides antibodies and antibody derivatives. In some embodiments, the present invention is based in part on the discovery of single domain antibodies that bind to OX40, which can be used in anti-tumor therapy, where the antibodies selectively activate OX40-mediated signaling pathways to induce immune cells to exert beneficial anti-tumor effects against tumor cells. In some embodiments, the antibodies disclosed herein are agonistic antibodies, which enhance OX40-mediated signaling pathways. In some embodiments, the anti-OX40 antibodies enhance the anti-tumor immune response of immune cells expressing OX40 protein. In some embodiments, the anti-OX40 antibodies include single domain antibodies, such as camelid antibodies or VHH antibodies. In some embodiments, the anti-OX40 antibodies have improved tissue penetration capabilities due to their smaller size compared to conventional antibodies in IgG, Fab and / or scFv format with the same potency.

[0091] In some embodiments, the antibodies of the invention may be or include monoclonal antibodies (including chimeric, humanized, or human antibodies). In some embodiments, the antibodies disclosed herein include humanized antibodies. In some embodiments, the antibodies include an acceptor human framework, such as a human immunoglobulin framework or a human shared framework.

[0092] In some embodiments, the antibody of the invention may be an antibody fragment, such as an Fv, Fab, Fab', scFv, diabody or F(ab')2 fragment. In some embodiments, the antibody is a full length antibody, such as a complete IgG1 antibody, or other antibody type or isotype as defined herein. In some embodiments, the antibody or antibody derivative of the invention may incorporate any of the features described herein (e.g., in Sections 2.1-2.12, described in detail herein), either alone or in combination.

[0093] The antibodies and antibody derivatives of the invention can be used, for example, in the diagnosis or treatment of neoplasms or cancers. In some embodiments, neoplasms and cancers whose growth can be inhibited using the antibodies of the invention include neoplasms and cancers that normally respond to immunotherapy. In some embodiments, the neoplasms and cancers include breast cancer (e.g., breast cell carcinoma), ovarian cancer (e.g., ovarian cell carcinoma), and renal cell carcinoma (RCC). Other examples of cancers that can be treated with the methods of the invention include melanoma (e.g., metastatic malignant melanoma), prostate cancer, colon cancer, lung cancer, bone cancer, pancreatic cancer, skin cancer, brain cancer, chronic or acute leukemia (including acute myeloid leukemia, chronic myeloid leukemia, acute lymphoblastic leukemia, chronic lymphocytic leukemia), lymphoma (e.g., Hodgkin's lymphoma and non-Hodgkin's lymphoma, lymphocytic lymphoma, primary CNS lymphoma, T-cell lymphoma), nasopharyngeal cancer, head or neck cancer, skin cancer or intraocular malignant melanoma, uterine cancer, rectal cancer, anal region cancer, gastric tumor, testicular cancer, uterine cancer, fallopian tube cancer, endometrial cancer, cervical cancer, vaginal cancer, external vaginal cancer, esophageal cancer, small intestine cancer, endocrine system cancer, thyroid cancer, parathyroid cancer, breast cancer, , soft tissue sarcoma, urethral cancer, penile cancer, childhood solid tumors, bladder cancer, kidney or ureteral cancer, carcinoma of the breast pelvis, central nervous system (CNS) neoplasms, tumor angiogenesis, spinal tumors, brain stem gliomas, pituitary adenomas, Kaposi's sarcoma, epidermoid carcinoma, squamous cell carcinoma, environmentally induced cancers including asbestos induced cancers (e.g., mesothelioma), and combinations of the above cancers.

[0094] 2.1 Exemplary Monospecific and Multispecific Antibodies 2.1.1 Exemplary Anti-OX40 Antibodies The present invention provides isolated antibodies that bind to OX40 protein. In some embodiments, the anti-OX40 antibodies of the present invention bind to the ECD of OX40. In some embodiments, the anti-OX40 antibodies bind to domain 2 (CRD2) and / or domain 4 (CRD4) of the ECD of OX40. In some embodiments, the anti-OX40 antibodies bind to domain 2 (CRD2) binding of the ECD of OX40. In some embodiments, the anti-OX40 antibodies bind to domain 4 (CRD4) of the ECD of OX40. In some embodiments, the ECD comprises the amino acid sequence set forth in SEQ ID NO:94. In some embodiments, the domain 2 (CRD2) of the ECD comprises the amino acid sequence set forth in SEQ ID NO:95. In some embodiments, the domain 4 (CRD4) of the ECD comprises the amino acid sequence set forth in SEQ ID NO:96. In some embodiments, the anti-OX40 antibodies bind to the same epitope as the anti-OX40 antibodies described herein (e.g., 1B3 or 2B7).

[0095] In some embodiments, the anti-OX40 antibodies disclosed herein may be agonists of OX40-mediated signaling pathways. In some embodiments, the anti-OX40 antibodies can enhance OX40 protein-dependent signaling pathways. In some embodiments, the anti-OX40 antibodies can increase the activity of the signaling pathway by at least about 10%, about 20%, about 30%, about 40%, about 50%, about 60%, about 70%, about 80%, about 90%, about 99% or about 99.9%. In some embodiments, treatment with the anti-OX40 antibodies exhibits an anti-tumor effect in a subject, thereby reducing tumor growth and / or prolonging the survival of the subject. In some embodiments, the anti-OX40 antibodies increase the immune response and / or anti-tumor effect of immune cells (e.g., T cells and / or NK cells expressing OX40) against tumor cells. In some embodiments, anti-OX40 antibodies, including single domain antibodies (e.g., VHH), have a smaller molecular size than full-length antibodies of the same potency, because the size of single domain antibodies is smaller than the Fab domain of full-length antibodies, which can cause better tissue infiltration, for example, at tumor sites, than full-length antibodies of the same potency. In some embodiments, treatment with anti-OX40 antibodies shows better anti-tumor effects than treatment with full-length anti-OX40 antibodies of the same potency.

[0096] In some embodiments, the anti-OX40 antibody comprises a single domain antibody that binds to OX40. In some embodiments, the single domain antibody comprises a VHH. In some embodiments, the single domain antibody comprises a heavy chain variable region (VH). In some embodiments, the single domain antibody is linked to an Fc region. In some embodiments, the single domain antibody is not linked to an Fc region.

[0097] In some embodiments, the single domain antibody is about 1x10 -7 In some embodiments, the single domain antibody binds to OX40 with a KD of about 1x10 -8In some embodiments, the single domain antibody binds to OX40 with a KD of about 5x10 -9 In some embodiments, the single domain antibody binds to OX40 with a KD of about 1x10 -9 In some embodiments, the single domain antibody binds to OX40 with a KD of about 1x10 -10 In some embodiments, the single domain antibody binds to OX40 with a KD of about 1x10 -11 M to approx. 1x10 -7 In some embodiments, the single domain antibody binds to OX40 with a KD of about 1x10 -10 M to approx. 1x10 -7 In some embodiments, the single domain antibody binds to OX40 with a KD of about 1x10 -10 M to approx. 1x10 -8 In some embodiments, the single domain antibody binds to OX40 with a KD of about 1x10 -11 M to approx. 1x10 -9 In some embodiments, the single domain antibody binds to OX40 with a KD of about 2x10 -10 M to approx. 5x10 -9 In some embodiments, the single domain antibody binds to OX40 with a KD of about 1x10 -9 M to approx. 5x10 -8 In some embodiments, the single domain antibody binds to OX40 with a KD of about 1x10 -10 M to approx. 1x10 -9 Binds to OX40 at the KD of M.

[0098] In some embodiments, the anti-OX40 antibody is bivalent, trivalent, tetravalent, pentavalent, hexavalent, heptavalent, or octavalent. In some embodiments, the anti-OX40 antibody is bivalent. In some embodiments, the anti-OX40 antibody is tetravalent. In some embodiments, the anti-OX40 antibody is hexavalent. In some embodiments, the anti-OX40 antibody comprises a heavy chain comprising a VHH domain and an Fc region. In some embodiments, the anti-OX40 antibody comprises a heavy chain comprising a VHH domain, a CH1 domain, and an Fc region. In some embodiments, the anti-OX40 antibody comprises a light chain comprising a VHH domain and a CL domain.

[0099] In some embodiments, the single domain antibody cross-competes with a reference single domain antibody for binding to OX40, the reference single domain antibody comprising a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO:1, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO:2, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO:3. In some embodiments, the single domain antibody cross-competes with a reference single domain antibody for binding to OX40, the reference single domain antibody comprising a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO:6, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO:7, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO:8. In some embodiments, the single domain antibody cross-competes with a reference single domain antibody for binding to OX40, the reference single domain antibody comprising a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO:11, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO:12, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO:13. In some embodiments, the single domain antibody cross-competes with a reference single domain antibody for binding to OX40, the reference single domain antibody comprising a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO:16, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO:17, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO:18. In some embodiments, the single domain antibody cross-competes with a reference single domain antibody for binding to OX40, the reference single domain antibody comprising a heavy chain variable region CDR1 comprising the amino acid sequence set forth in SEQ ID NO:21, a heavy chain variable region CDR2 comprising the amino acid sequence set forth in SEQ ID NO:22, and a heavy chain variable region CDR3 comprising the amino acid sequence set forth in SEQ ID NO:23.In some embodiments, the single domain antibody cross-competes with a reference single domain antibody for binding to OX40, the reference single domain antibody comprising a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO:26, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO:27, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO:28. In some embodiments, the single domain antibody cross-competes with a reference single domain antibody for binding to OX40, the reference single domain antibody comprising a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO:31, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO:32, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO:33. In some embodiments, the single domain antibody cross-competes with a reference single domain antibody for binding to OX40, the reference single domain antibody comprising a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO:36, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO:37, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO:38. In some embodiments, the single domain antibody cross-competes with a reference single domain antibody for binding to OX40, the reference single domain antibody comprising a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO:41, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO:42, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO:43. In some embodiments, the single domain antibody cross-competes with a reference single domain antibody for binding to OX40, the reference single domain antibody comprising a heavy chain variable region CDR1 comprising the amino acid sequence depicted in SEQ ID NO:46, a heavy chain variable region CDR2 comprising the amino acid sequence depicted in SEQ ID NO:47, and a heavy chain variable region CDR3 comprising the amino acid sequence depicted in SEQ ID NO:48.In some embodiments, the single domain antibody cross-competes with a reference single domain antibody for binding to OX40, the reference single domain antibody comprising a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO:51, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO:52, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO:53. In some embodiments, the single domain antibody cross-competes with a reference single domain antibody for binding to OX40, the reference single domain antibody comprising a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO:56, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO:57, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO:58. In some embodiments, the single domain antibody cross-competes with a reference single domain antibody for binding to OX40, the reference single domain antibody comprising a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO:61, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO:62, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO:63. In some embodiments, the single domain antibody cross-competes with a reference single domain antibody for binding to OX40, the reference single domain antibody comprising a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO:66, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO:67, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO:68. In some embodiments, the single domain antibody cross-competes with a reference single domain antibody for binding to OX40, the reference single domain antibody comprising a heavy chain variable region CDR1 comprising the amino acid sequence set forth in SEQ ID NO:71, a heavy chain variable region CDR2 comprising the amino acid sequence set forth in SEQ ID NO:72, and a heavy chain variable region CDR3 comprising the amino acid sequence set forth in SEQ ID NO:73.In some embodiments, the single domain antibody cross-competes with a reference single domain antibody for binding to OX40, the reference single domain antibody comprising a heavy chain variable region CDR1 comprising the amino acid sequence set forth in SEQ ID NO:76, a heavy chain variable region CDR2 comprising the amino acid sequence set forth in SEQ ID NO:77, and a heavy chain variable region CDR3 comprising the amino acid sequence set forth in SEQ ID NO:78.

[0100] In some embodiments, the single domain antibody comprises a heavy chain variable region comprising: a) a heavy chain variable region CDR1 comprising the amino acid sequence of any one of SEQ ID NOs: 1, 6, 11, 16, 21, 26, 31, 36, 41, 46, 51, 56, 61, 66, 71 or 76, or a variant of said amino acid sequence comprising at most about three amino acid substitutions; b) a heavy chain variable region CDR2 comprising the amino acid sequence of any one of SEQ ID NOs: 2, 7, 12, 17, 22, 27, 32, 37, 42, 47, 52, 57, 62, 67, 72 or 77, or a variant of said amino acid sequence comprising at most about three amino acid substitutions; and c) a heavy chain variable region CDR3 comprising the amino acid sequence of any one of SEQ ID NOs: and a heavy chain variable region CDR3 comprising any one of the amino acid sequences of 3, 8, 13, 18, 23, 28, 33, 38, 43, 48, 53, 58, 63, 68, 73 or 78, or a variant of said amino acid sequence comprising at most about three amino acid substitutions.

[0101] In some embodiments, the single domain antibody comprises a heavy chain variable region comprising a CDR1 domain, a CDR2 domain and a CDR3 domain, wherein the CDR1 domain, the CDR2 domain and the CDR3 domain respectively comprise the CDR1 domain, the CDR2 domain and the CDR3 domain comprised in a reference heavy chain variable region, wherein the reference heavy chain variable region comprises an amino acid sequence selected from SEQ ID NO: 4, 9, 14, 19, 24, 29, 34, 39, 44, 49, 54, 59, 64, 69, 74 and 79.

[0102] In some embodiments, the single domain antibody comprises a heavy chain variable region CDR1 comprising the amino acid sequence set forth in SEQ ID NO:1, a heavy chain variable region CDR2 comprising the amino acid sequence set forth in SEQ ID NO:2, and a heavy chain variable region CDR3 comprising the amino acid sequence set forth in SEQ ID NO:3. In some embodiments, the single domain antibody comprises a heavy chain variable region CDR1 comprising the amino acid sequence set forth in SEQ ID NO:6, a heavy chain variable region CDR2 comprising the amino acid sequence set forth in SEQ ID NO:7, and a heavy chain variable region CDR3 comprising the amino acid sequence set forth in SEQ ID NO:8. In some embodiments, the single domain antibody comprises a heavy chain variable region CDR1 comprising the amino acid sequence set forth in SEQ ID NO:11, a heavy chain variable region CDR2 comprising the amino acid sequence set forth in SEQ ID NO:12, and a heavy chain variable region CDR3 comprising the amino acid sequence set forth in SEQ ID NO:13. In some embodiments, the single domain antibody comprises a heavy chain variable region CDR1 comprising the amino acid sequence set forth in SEQ ID NO: 16, a heavy chain variable region CDR2 comprising the amino acid sequence set forth in SEQ ID NO: 17, and a heavy chain variable region CDR3 comprising the amino acid sequence set forth in SEQ ID NO: 18. In some embodiments, the single domain antibody comprises a heavy chain variable region CDR1 comprising the amino acid sequence set forth in SEQ ID NO: 21, a heavy chain variable region CDR2 comprising the amino acid sequence set forth in SEQ ID NO: 22, and a heavy chain variable region CDR3 comprising the amino acid sequence set forth in SEQ ID NO: 23. In some embodiments, the single domain antibody comprises a heavy chain variable region CDR1 comprising the amino acid sequence set forth in SEQ ID NO: 26, a heavy chain variable region CDR2 comprising the amino acid sequence set forth in SEQ ID NO: 27, and a heavy chain variable region CDR3 comprising the amino acid sequence set forth in SEQ ID NO: 28. In some embodiments, the single domain antibody comprises a heavy chain variable region CDR1 comprising the amino acid sequence depicted in SEQ ID NO:31, a heavy chain variable region CDR2 comprising the amino acid sequence depicted in SEQ ID NO:32, and a heavy chain variable region CDR3 comprising the amino acid sequence depicted in SEQ ID NO:33.In some embodiments, the single domain antibody comprises a heavy chain variable region CDR1 comprising the amino acid sequence set forth in SEQ ID NO: 36, a heavy chain variable region CDR2 comprising the amino acid sequence set forth in SEQ ID NO: 37, and a heavy chain variable region CDR3 comprising the amino acid sequence set forth in SEQ ID NO: 38. In some embodiments, the single domain antibody comprises a heavy chain variable region CDR1 comprising the amino acid sequence set forth in SEQ ID NO: 41, a heavy chain variable region CDR2 comprising the amino acid sequence set forth in SEQ ID NO: 42, and a heavy chain variable region CDR3 comprising the amino acid sequence set forth in SEQ ID NO: 43. In some embodiments, the single domain antibody comprises a heavy chain variable region CDR1 comprising the amino acid sequence set forth in SEQ ID NO: 46, a heavy chain variable region CDR2 comprising the amino acid sequence set forth in SEQ ID NO: 47, and a heavy chain variable region CDR3 comprising the amino acid sequence set forth in SEQ ID NO: 48. In some embodiments, the single domain antibody comprises a heavy chain variable region CDR1 comprising the amino acid sequence set forth in SEQ ID NO:51, a heavy chain variable region CDR2 comprising the amino acid sequence set forth in SEQ ID NO:52, and a heavy chain variable region CDR3 comprising the amino acid sequence set forth in SEQ ID NO:53. In some embodiments, the single domain antibody comprises a heavy chain variable region CDR1 comprising the amino acid sequence set forth in SEQ ID NO:56, a heavy chain variable region CDR2 comprising the amino acid sequence set forth in SEQ ID NO:57, and a heavy chain variable region CDR3 comprising the amino acid sequence set forth in SEQ ID NO:58. In some embodiments, the single domain antibody comprises a heavy chain variable region CDR1 comprising the amino acid sequence set forth in SEQ ID NO:61, a heavy chain variable region CDR2 comprising the amino acid sequence set forth in SEQ ID NO:62, and a heavy chain variable region CDR3 comprising the amino acid sequence set forth in SEQ ID NO:63. In some embodiments, the single domain antibody comprises a heavy chain variable region CDR1 comprising the amino acid sequence depicted in SEQ ID NO:66, a heavy chain variable region CDR2 comprising the amino acid sequence depicted in SEQ ID NO:67, and a heavy chain variable region CDR3 comprising the amino acid sequence depicted in SEQ ID NO:68.In some embodiments, the single domain antibody comprises a heavy chain variable region CDR1 comprising the amino acid sequence set forth in SEQ ID NO:71, a heavy chain variable region CDR2 comprising the amino acid sequence set forth in SEQ ID NO:72, and a heavy chain variable region CDR3 comprising the amino acid sequence set forth in SEQ ID NO:73. In some embodiments, the single domain antibody comprises a heavy chain variable region CDR1 comprising the amino acid sequence set forth in SEQ ID NO:76, a heavy chain variable region CDR2 comprising the amino acid sequence set forth in SEQ ID NO:77, and a heavy chain variable region CDR3 comprising the amino acid sequence set forth in SEQ ID NO:78.

[0103] In some embodiments, a single domain antibody comprises a heavy chain variable region comprising an amino acid sequence having at least about 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% sequence identity to an amino acid sequence selected from SEQ ID NOs: 4, 9, 14, 19, 24, 29, 34, 39, 44, 49, 54, 59, 64, 69, 74 and 79. In some embodiments, a single domain antibody comprises a heavy chain variable region comprising an amino acid sequence selected from SEQ ID NOs: 4, 9, 14, 19, 24, 29, 34, 39, 44, 49, 54, 59, 64, 69, 74 and 79.

[0104] In some embodiments, the single domain antibody comprises a heavy chain variable region comprising the amino acid sequence set forth in SEQ ID NO:4. In some embodiments, the single domain antibody comprises a heavy chain variable region comprising the amino acid sequence set forth in SEQ ID NO:9. In some embodiments, the single domain antibody comprises a heavy chain variable region comprising the amino acid sequence set forth in SEQ ID NO:14. In some embodiments, the single domain antibody comprises a heavy chain variable region comprising the amino acid sequence set forth in SEQ ID NO:19. In some embodiments, the single domain antibody comprises a heavy chain variable region comprising the amino acid sequence set forth in SEQ ID NO:24. In some embodiments, the single domain antibody comprises a heavy chain variable region comprising the amino acid sequence set forth in SEQ ID NO:29. In some embodiments, the single domain antibody comprises a heavy chain variable region comprising the amino acid sequence set forth in SEQ ID NO:34. In some embodiments, the single domain antibody comprises a heavy chain variable region comprising the amino acid sequence set forth in SEQ ID NO:39. In some embodiments, the single domain antibody comprises a heavy chain variable region comprising the amino acid sequence set forth in SEQ ID NO:44. In some embodiments, the single domain antibody comprises a heavy chain variable region comprising the amino acid sequence set forth in SEQ ID NO: 49. In some embodiments, the single domain antibody comprises a heavy chain variable region comprising the amino acid sequence set forth in SEQ ID NO: 54. In some embodiments, the single domain antibody comprises a heavy chain variable region comprising the amino acid sequence set forth in SEQ ID NO: 59. In some embodiments, the single domain antibody comprises a heavy chain variable region comprising the amino acid sequence set forth in SEQ ID NO: 64. In some embodiments, the single domain antibody comprises a heavy chain variable region comprising the amino acid sequence set forth in SEQ ID NO: 69. In some embodiments, the single domain antibody comprises a heavy chain variable region comprising the amino acid sequence set forth in SEQ ID NO: 74. In some embodiments, the single domain antibody comprises a heavy chain variable region comprising the amino acid sequence set forth in SEQ ID NO: 79.

[0105] In some embodiments, the anti-OX40 antibody comprises a heavy chain comprising an amino acid sequence having at least about 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% sequence identity to an amino acid sequence selected from SEQ ID NOs: 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, and 81-92. In some embodiments, the single domain antibody comprises a heavy chain comprising an amino acid sequence selected from SEQ ID NOs: 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, and 81-92. In some embodiments, the anti-OX40 antibody comprises a heavy chain comprising an amino acid sequence set forth in SEQ ID NO:5. In some embodiments, the anti-OX40 antibody comprises a heavy chain comprising the amino acid sequence depicted in SEQ ID NO:10. In some embodiments, the anti-OX40 antibody comprises a heavy chain comprising the amino acid sequence depicted in SEQ ID NO:15. In some embodiments, the anti-OX40 antibody comprises a heavy chain comprising the amino acid sequence depicted in SEQ ID NO:20. In some embodiments, the anti-OX40 antibody comprises a heavy chain comprising the amino acid sequence depicted in SEQ ID NO:25. In some embodiments, the anti-OX40 antibody comprises a heavy chain comprising the amino acid sequence depicted in SEQ ID NO:30. In some embodiments, the anti-OX40 antibody comprises a heavy chain comprising the amino acid sequence depicted in SEQ ID NO:35. In some embodiments, the anti-OX40 antibody comprises a heavy chain comprising the amino acid sequence depicted in SEQ ID NO:40. In some embodiments, the anti-OX40 antibody comprises a heavy chain comprising the amino acid sequence depicted in SEQ ID NO:45. In some embodiments, the anti-OX40 antibody comprises a heavy chain comprising the amino acid sequence depicted in SEQ ID NO:50. In some embodiments, the anti-OX40 antibody comprises a heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 55. In some embodiments, the anti-OX40 antibody comprises a heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 60. In some embodiments, the anti-OX40 antibody comprises a heavy chain comprising the amino acid sequence set forth in SEQ ID NO: 65.In some embodiments, the anti-OX40 antibody comprises a heavy chain comprising the amino acid sequence depicted in SEQ ID NO:70. In some embodiments, the anti-OX40 antibody comprises a heavy chain comprising the amino acid sequence depicted in SEQ ID NO:75. In some embodiments, the anti-OX40 antibody comprises a heavy chain comprising the amino acid sequence depicted in SEQ ID NO:80. In some embodiments, the anti-OX40 antibody comprises a heavy chain comprising the amino acid sequence depicted in SEQ ID NO:81. In some embodiments, the anti-OX40 antibody comprises a heavy chain comprising the amino acid sequence depicted in SEQ ID NO:83. In some embodiments, the anti-OX40 antibody comprises a heavy chain comprising the amino acid sequence depicted in SEQ ID NO:85. In some embodiments, the anti-OX40 antibody comprises a heavy chain comprising the amino acid sequence depicted in SEQ ID NO:87. In some embodiments, the anti-OX40 antibody comprises a heavy chain comprising the amino acid sequence depicted in SEQ ID NO:89. In some embodiments, the anti-OX40 antibody comprises a heavy chain comprising the amino acid sequence depicted in SEQ ID NO:90. In some embodiments, the anti-OX40 antibody comprises a heavy chain comprising the amino acid sequence set forth in SEQ ID NO:91. In some embodiments, the anti-OX40 antibody comprises a heavy chain comprising the amino acid sequence set forth in SEQ ID NO:92. In some embodiments, the anti-OX40 antibody comprises a light chain comprising the amino acid sequence set forth in SEQ ID NO:82. In some embodiments, the anti-OX40 antibody comprises a light chain comprising the amino acid sequence set forth in SEQ ID NO:84. In some embodiments, the anti-OX40 antibody comprises a light chain comprising the amino acid sequence set forth in SEQ ID NO:86. In some embodiments, the anti-OX40 antibody comprises a light chain comprising the amino acid sequence set forth in SEQ ID NO:88.

[0106] In some embodiments, the anti-OX40 antibody comprises a heavy chain comprising an amino acid sequence selected from SEQ ID NO:81, 83, 85, and 87. In some embodiments, the anti-OX40 antibody comprises a light chain comprising an amino acid sequence selected from SEQ ID NO:82, 84, 86, and 88. In some embodiments, the anti-OX40 antibody comprises a heavy chain comprising an amino acid sequence set forth in SEQ ID NO:81 and a light chain comprising an amino acid sequence set forth in SEQ ID NO:82. In some embodiments, the anti-OX40 antibody comprises a heavy chain comprising an amino acid sequence set forth in SEQ ID NO:83 and a light chain comprising an amino acid sequence set forth in SEQ ID NO:84. In some embodiments, the anti-OX40 antibody comprises a heavy chain comprising an amino acid sequence set forth in SEQ ID NO:85 and a light chain comprising an amino acid sequence set forth in SEQ ID NO:86. In some embodiments, the anti-OX40 antibody comprises a heavy chain comprising an amino acid sequence set forth in SEQ ID NO:87 and a light chain comprising an amino acid sequence set forth in SEQ ID NO:88. In some embodiments, the anti-OX40 antibody comprises a heavy chain comprising the amino acid sequence set forth in SEQ ID NO:81 and a light chain comprising the amino acid sequence set forth in SEQ ID NO:84. In some embodiments, the anti-OX40 antibody comprises a heavy chain comprising the amino acid sequence set forth in SEQ ID NO:83 and a light chain comprising the amino acid sequence set forth in SEQ ID NO:82.

[0107] In some embodiments, any one of the amino acid sequences contained in the heavy chain variable region may contain at most about 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 amino acid substitutions, deletions, and / or additions. In some embodiments, the amino acid substitutions are conservative substitutions.

[0108] In some embodiments, the single domain antibody comprises a humanized framework, hi some embodiments, the humanized framework comprises the framework sequence of the heavy chain variable region sequence shown in SEQ ID NO: 9, 14, 19, 24, 29, 34, 39, 44, 49, 54, 59, 64, 69, 74 or 79.

[0109] In some embodiments, the anti-OX40 antibody does not comprise an Fc region. In some embodiments, the anti-OX40 antibody further comprises an Fc region. In some embodiments, the Fc region comprises a human Fc region. In some embodiments, the Fc region comprises an Fc region selected from an IgG, IgA, IgD, IgE, and IgM Fc region. In some embodiments, the Fc region comprises an Fc region selected from an IgG1, IgG2, IgG3, and IgG4 Fc region. In some embodiments, the Fc region comprises an IgG1 Fc region. In some embodiments, the Fc region comprises an IgG2 Fc region. In some embodiments, the Fc region comprises an IgG4 Fc region. In some embodiments, the Fc region comprises one or more amino acid modifications, substitutions, or mutations described in Section 2.7.3.

[0110] In some embodiments, the heavy chain variable region is linked to the Fc region via a linker. In some embodiments, the linker is a peptide linker. In some embodiments, the peptide linker comprises about 4 to about 30 amino acids. In some embodiments, the peptide linker comprises about 4 to about 15 amino acids. In some embodiments, the peptide linker comprises an amino acid sequence selected from SEQ ID NOs: 97-140.

[0111] In some embodiments, the anti-OX40 antibody comprises a full-length immunoglobulin, a single chain Fv (scFv) fragment, a Fab fragment, a Fab' fragment, a F(ab')2, an Fv fragment, a disulfide stabilized Fv fragment (dsFv), (dsFv)2, a VHH, an Fv-Fc fusion, an scFv-Fc fusion, a VHH-Fv fusion, a diabody, a triabody, a tetrabody, or any combination thereof.

[0112] In some embodiments, the antibody is included in a larger molecule as an antibody derivative. In some embodiments, the antibody derivative is a multispecific antibody, e.g., a bispecific antibody, where the multispecific antibody comprises a second antibody portion that specifically binds to a second antigen. In some embodiments, the second antigen is a tumor-associated antigen. In some embodiments, the tumor associated antigen is selected from the group consisting of Her-2, EGFR, PD-L1, MSLN, c-Met, B-cell maturation antigen (BCMA), carbonic anhydrase IX (CA1X), carcinoembryonic antigen (CEA), CD5, CD7, CD10, CD19, CD20, CD22, CD30, CD33, CD34, CD38, CD41, CD44, CD47, CD49f, CD56, CD74, CD123, CD133, CD138, CD276 (B7H3), epithelial glycoprotein (EGP2), trophoblast cell surface antigen 2 (TROP-2), epithelial glycoprotein-40 (EGP-40), epithelial cell adhesion molecule (EpCAM), receptor tyrosine protein kinase erb-B2, 3, 4, folate binding protein (FBP), fetal acetylcholine receptor (AChR), folate receptor-a, galectin receptor-2 (GAMMA), folate receptor-β ... The second antigen is selected from ganglioside G2 (GD2), ganglioside G3 (GD3), human telomerase reverse transcriptase (hTERT), kinase insert domain receptor (KDR), LewisA (CA1.9.9), LewisY (LeY), L1 cell adhesion molecule (L1CAM), mucin 16 (Muc-16), mucin 1 (Muc-1), NG2D ligand, oncofetal antigen (h5T4), prostate stem cell antigen (PSCA), prostate specific membrane antigen (PSMA), tumor associated glycoprotein 72 (TAG-72), Claudin 18.2 (CLDN18.2), vascular endothelial growth factor R2 (VEGF-R2), Wilms tumor protein (WT-1), tyrosine protein kinase transmembrane receptor type 1 (ROR1), PVR, PVRL2, GPC3, and any combination thereof. In some embodiments, the second antigen is an immune checkpoint modulator. In some embodiments, the immune checkpoint modulating agent is selected from TIGIT, PD1, CTLA4, LAG-3, 2B4, BTLA, and any combination thereof.In some embodiments, binding of the antibody derivative or multispecific antibody to the second antigen inhibits the immune checkpoint modulator. In some embodiments, the second antigen is an immune costimulatory molecule or a subunit of the T cell receptor / CD3 complex. In some embodiments, the immune costimulatory molecule is selected from CD28, ICOS, CD27, 4-1BB, OX40, and CD40, and any combination thereof. In some embodiments, binding of the antibody derivative or multispecific antibody to the second antigen activates the immune costimulatory molecule. In some embodiments, the subunit of the T cell receptor / CD3 complex is selected from the group consisting of CD3γ, CD3δ, CD3ε, and any combination thereof. In some embodiments, binding of the antibody derivative or multispecific antibody to the second antigen activates the T cell receptor / CD3 complex.

[0113] In some embodiments, the anti-OX40 antibody is linked to the second antigen binding moiety via a linker. In some embodiments, the linker is a peptide linker. In some embodiments, the peptide linker comprises about 4 to about 30 amino acids. In some embodiments, the peptide linker comprises about 4 to about 15 amino acids. In some embodiments, the peptide linker comprises an amino acid sequence selected from SEQ ID NOs: 97-140.

[0114] In some embodiments, the anti-OX40 antibody is conjugated to a therapeutic agent or label. In some embodiments, the label is selected from a radioisotope, a fluorescent dye, and an enzyme.

[0115] 2.2 Antibody affinity In some embodiments, the antibodies or antibody derivatives disclosed herein have high binding affinity for their target antigens. In some embodiments, the antibodies or antibody derivatives have a binding affinity of about 1x10 -7 In some embodiments, the antibody or antibody derivative binds to a target with a KD of about 1x10 -8In some embodiments, the antibody or antibody derivative binds to a target with a KD of about 5x10 -9 In some embodiments, the antibody or antibody derivative binds to a target with a KD of about 1x10 -9 In some embodiments, the antibody or antibody derivative binds to a target with a KD of about 1x10 -10 It binds to a target with a KD smaller than M.

[0116] In some embodiments, the antibody or antibody derivative is about 1x10 -11 M to approx. 1x10 -7 In some embodiments, the antibody or antibody derivative binds to the target with a KD of about 1x10 -10 M to approx. 1x10 -7 In some embodiments, the antibody or antibody derivative binds to the target with a KD of about 1x10 -10 M to approx. 1x10 -8 In some embodiments, the antibody or antibody derivative binds to the target with a KD of about 1x10 -11 M to approx. 1x10 -9 In some embodiments, the antibody or antibody derivative binds to a target with a KD of about 2x10 -10 M to approx. 5x10 -9 In some embodiments, the antibody or antibody derivative binds to the target with a KD of about 1x10 -9 M to approx. 5x10 -8 In some embodiments, the antibody or antibody derivative binds to the target with a KD of about 1x10 -10 M to approx. 1x10 -9 It binds to the target with a KD of M.

[0117] The KD of an antibody or antibody derivative may be measured by methods known in the art, including but not limited to Western blot, ELISA-, RIA-, ECL-, IRMA-, EIA-, Octet-BIACORE® assays and peptide scanning.

[0118] In some embodiments, KD may be measured using a BIACORE® surface plasmon resonance assay. For example, but not limited to, measurements are performed using a BIACORE® 3000 (Biacore, Piscataway, NJ) at 25° C. with an immobilized antigen CMS chip at about 10 response units (RU). In some embodiments, a carboxymethylated dexamethasone biosensor chip (CMS, Biacore) is activated with N-ethyl-N'-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) according to the supplier's manual. The antigen is diluted to 5 μg / ml (about 0.2 μM) in 10 mM sodium acetate (pH 4.8) and injected at a flow rate of 5 μl / min to achieve about 10 response units (RU) of coupled protein. After antigen injection, 1 M ethanolamine is injected to block unreacted groups. To perform kinetic measurements, two-fold serial dilutions of Fab (0.78 nM to 500 nM) are injected in PBS with 0.05% polysorbate 20 (TWEEN®-20™) surfactant (PBST) at a flow rate of approximately 25 μl / min at 25° C. Association rates (k on ) and dissociation rate (k off The equilibrium dissociation constant (KD) may be calculated as the ratio of koff / kon. See, e.g., Chen et al., J. Mol. Biol. 293:865-881 (1999). When the binding rate measured by the surface plasmon resonance assay is 10 6 M -l s -1Above 100 nm, the association rate may be determined with a fluorescence quenching technique, which measures the increase or decrease in fluorescence emission intensity (excitation = 295 nm, emission = 340 nm, 16 nm bandpass) in PBS, pH 7.2, at 25°C, of ​​20 nM anti-antigen antibody (Fab format) in the presence of increasing antigen concentrations, which are measured using a spectrometer, e.g. a spectrophotometer in pass-cut configuration (Aviv Instruments) or a 8000 series SLM-AMINCO with a stirred absorber pool. TM Measured by spectrophotometer (ThermoSpectronic).

[0119] 2.3 Antibody fragments In some embodiments, the antibodies of the invention include antigen-binding or antibody fragments. Antibody fragments include, but are not limited to, Fab, Fab', Fab'-SH, F(ab')2, VHH, Fv, and scFv fragments, and other fragments described herein. For a review of some antibody fragments, see Hudson et al. Nat. Med. 9: 129-134 (2003). For a review of scFv fragments, see, e.g., Pluckthtin, The Pharmacology of Monoclonal Antibodies, vol. 113, Rosenburg and Moore eds., (Springer Verlag, New York), pp. 269-315 (1994), and may also refer to WO 93 / 16185, and U.S. Patent Nos. 5,571,894 and 5,587,458. For a discussion of Fab and F(ab)2 fragments that contain rescue receptor binding epitope residues and have increased in vivo half-lives, see US Pat. No. 5,869,046.

[0120] In some embodiments, the antibody of the present invention may be a diabody. Diabodies are antibody fragments that have two antigen-binding sites, which may be bivalent or bispecific. See, e.g., EP 404,097, WO 1993 / 01161, Hudson et al., Nat. Med. 9:129-134 (2003), and Hollinger et al., Proc. Natl. Acad. Sci. USA 90: 6444-6448 (1993). Triabodies and tetrabodies are also described in Hudson et al., Nat. Med. 9: 129-134 (2003).

[0121] In some embodiments, an antibody of the invention may comprise a single domain antibody. A single domain antibody is an antibody fragment that comprises all or a portion of the heavy chain variable region or all or a portion of the light chain variable region of an antibody. In some embodiments, a single domain antibody is a human single domain antibody (Domantis, Inc., Waltham, MA, see e.g., U.S. Pat. No. 6,248,516 B1). In some embodiments, a single domain antibody is a camelid single domain antibody. In some embodiments, a single domain antibody is a VHH. In some embodiments, a single domain antibody is humanized.

[0122] Antibody fragments may be prepared by a variety of techniques, including, but not limited to, proteolytic digestion of whole antibodies and production by recombinant host cells (e.g., E. coli or phages), as described herein.

[0123] 2.4 Chimeric and humanized antibodies In some embodiments, the antibody of the present invention is a chimeric antibody. Some chimeric antibodies are described, for example, in U.S. Pat. No. 4,816,567, and Morrison et al., Proc. Natl. Acad. Sci. USA, 81:6851-6855 (1984). In some embodiments, a chimeric antibody comprises a non-human variable region (e.g., a variable region derived from a mouse) and a human constant region. In some embodiments, a chimeric antibody is a "class-switched" antibody, in which the class or subclass has been changed from that of the parent antibody. Chimeric antibodies include antigen-binding fragments thereof.

[0124] In some embodiments, the antibody of the present invention may be a humanized antibody. Humanization is typically performed on a non-human antibody to reduce immunogenicity to humans while retaining the specificity and affinity of the parent non-human antibody. Typically, a humanized antibody comprises one or more variable domains, in which the HVRs, e.g., CDRs (or a portion thereof), are derived from a non-human antibody, and one or more framework (FR) (or any portion thereof) are derived from a human antibody sequence. The humanized antibody may optionally further comprise at least a portion of a human constant region. In some embodiments, some FR residues in the humanized antibody are replaced by corresponding residues from a non-human antibody (e.g., an antibody derived from HVR residues), e.g., to restore or improve antibody specificity or affinity.

[0125] Humanized antibodies and methods for their preparation are described, for example, in Almagro and Fransson, Front. Biosci. 13:1619-1633 (2008), and further described, for example, in Riechmann et al., Nature 332:323-329 (1988); Queen et al., Proc. Nat'l Acad. Sci. USA 86:10029-10033 (1989), U.S. Pat. Nos. 5,821,337, 7,527,791, 6,982,321, and 7,087,409, Kashmiri et al., Methods 36:25-34 (2005) (SDR (a-CDR) grafting is described), Padlan, Mol. Immunol. 28:489-498 (1991). (describing "resurfacing"), Dall'Acqua et al., Methods 36:43-60 (2005) (describing "FR shuffling"), and Osbourn et al., Methods 36:61-68 (2005) and Klimka et al., Br. J. Cancer, 83:252-260 (2000) (describing a "guide selection" method for FR shuffling).

[0126] Human framework regions that may be used for humanization include framework regions selected by the "best-fit" method (see, e.g., Sims et al. J. Immunol. 151:2296 (1993)), framework regions derived from shared sequences of human antibodies of a particular subclass of light or heavy chain variable region (see, e.g., Carter et al. Proc. Natl. Acad. Sci. USA,89:4285 (1992), and Presta et al. J. Immunol.,151:2623 (1993)), human mature (somatically mutated) framework regions or human germline framework regions (see, e.g., Almagro and Fransson, Front. Biosci. 13:1619-1633 (2008)), and framework regions obtained by screening FR libraries (see, e.g., Baca et al., J. Biol. Chem. 272:10678-10684 (2008)). (1997) and Rosok et al., J. Biol. Chem. 271:22611-22618 (1996)).

[0127] 2.5 Human antibodies In some embodiments, the antibodies of the invention may be human antibodies (e.g., human domain antibodies or human DAbs). Human antibodies may be produced using a variety of different techniques known in the art. Human antibodies are generally described in van Dijk and van de Winkel, Curr. Opin. Pharmacol. 5: 368-74 (2001); Lonberg, Curr. Opin. Immunol. 20:450-459 (2008); and Chen, Mol. Immunol. 47(4):912-21 (2010). Transgenic mice or rats capable of producing fully human single domain antibodies (or DAbs) are known in the art. See, for example, US20090307787A1, U.S. Patent No. 8,754,287, US20150289489A1, US20100122358A1, and WO 2004049794.

[0128] Human antibodies (e.g., human DAbs) can be prepared by administering an immunogen to a transgenic animal that has been modified to produce fully human antibodies or complete antibodies with human variable regions in response to antigenic challenge. Such animals typically contain all or part of the human immunoglobulin loci, which replace endogenous immunoglobulin loci or are present extrachromosomally or randomly integrated into the animal's chromosomes. In such transgenic mice, the endogenous immunoglobulin loci are typically inactivated. For a review of methods for obtaining human antibodies from transgenic animals, see Lonberg, Nat. Biotech. 23:1117-1125 (2005). Also see, e.g., XENOMOUSE. TMSee U.S. Patent Nos. 6,075,181 and 6,150,584, which describe HuMab® technology, U.S. Patent No. 5,770,429, which describes HuMab® technology, U.S. Patent No. 7,041,870, which describes KM MOUSE® technology, and U.S. Patent Application Publication No. US2007 / 0061900, which describes VelociMouse® technology. The human variable regions of the complete antibodies produced by such animals may be further modified, for example, by conjugation to different human constant regions.

[0129] Human antibodies (e.g., human DAbs) may be prepared by hybridoma-based methods. Human myeloma and mouse-human heteromyeloma cell lines for producing human monoclonal antibodies have been described (see, e.g., Kozbor J. Immunol., 133: 3001 (1984); Brodeur et al., Monoclonal Antibody Production Techniques and Applications, pp. 51-63 (Marcel Dekker, Inc., New York, 1987); and Boerner et al., J. Immunol., 147: 86 (1991)). Human antibodies produced by human B-cell hybridoma technology have also been described in Li et al., Proc. Natl. Acad. Sci. USA, 103:3557-3562 (2006). Other methods include those described, for example, in U.S. Patent No. 7,189,826 (which describes the production of monoclonal human IgM antibodies from hybridoma cell lines) and Ni, Modern Immunology, 26(4):265-268 (2006) (which describes human-human hybridomas). Human hybridoma technology (Trioma technology) is also described in Vollmers and Brandlein, Histology and Histopathology, 20(3):927-937 (2005) and Vollmers and Brandlein, Methods and Findings in Experimental and Clinical Pharmacology, 27(3):185-91 (2005).

[0130] Human antibodies (e.g., human DAbs) may be produced by isolating Fv clone variable domain sequences selected from a human phage display library. Such variable domain sequences may then be combined with the necessary human constant domains. A description of techniques for selecting human antibodies from an antibody library follows.

[0131] 2.6 Library-derived antibodies Combinatorial libraries can be screened for antibodies with the desired activity or activities to isolate the antibodies of the invention. For example, several methods are known in the art for producing phage display libraries and screening such libraries for antibodies with the required binding properties. Such methods are described, for example, in Hoogenboom et al. Methods in Molecular Biology 178:1-37 (O'Brien et al., ed., Human Press, Totowa, NJ, 2001), and further in McCafferty et al., Nature 348:552-554, Clackson et al., Nature 352: 624-628 (1991), Marks et al., J. Mol. Biol. 222: 581-597 (1992), Marks and Bradbury, Methods in Molecular Biology 248:161-175 (Lo, ed., Human Press, Totowa, NJ, 2003), Sidhu et al., J. Mol. Biol. 338(2): 299-310 (2004), Lee et al., J. Mol. Biol. 340(5): 1073-1093 (2004), Fellouse, Proc. Natl. Acad. Sci. USA 101(34): 12467-12472 (2004), Lee et al., J. Immunol. Methods 284(1-2): 119-132(2004). Methods for constructing single domain antibody libraries have already been described in U.S. Patent No. 7,371,849.

[0132] In some phage display methods, V H and V LThe gene libraries can be cloned respectively, randomly recombined in a phage library, and screened for antigen-binding phages as described in Winter et al., Ann. Rev. Immunol., 12: 433-455 (1994). Phages usually display antibody fragments as scFv fragments or Fab fragments. Libraries from immune sources can provide high affinity antibodies to immunogens without constructing hybridomas. Alternatively, naive libraries can be cloned (e.g., from humans) without the need for any immunization, to provide a single source of antibodies against a wide range of non-self and self antigens, as described in Griffiths et al., EMBO J, 12: 725-734 (1993). Finally, naive libraries can be synthesized by cloning unrearranged V gene segments from stem cells and using PCR primers containing random sequences to encode highly variable CDR3 regions and complete rearrangement in vitro, as described in Hoogenboom and Winter, J. Mol. Biol., 227: 381-388 (1992). Patent publications describing human antibody phage libraries include, for example, U.S. Patent No. 5,750,373 and U.S. Patent Publication Nos. 2005 / 0079574, 2005 / 0119455, 2005 / 0266000, 2007 / 0117126, 2007 / 0160598, 2007 / 0237764, 2007 / 0292936, and 2009 / 0002360.

[0133] Antibodies or antibody fragments isolated from a human antibody library are considered human antibodies or human antibody fragments herein.

[0134] 2.7 Antibody variants The present invention further provides amino acid sequence variants of the disclosed antibodies. For example, it may be necessary to improve the binding affinity and / or other biological properties of the antibody. Amino acid sequence variants of the antibody may be prepared by introducing appropriate modifications into the nucleotide sequence encoding the antibody or by peptide synthesis. Such modifications include, but are not limited to, deletion and / or insertion and / or substitution of residues within the amino acid sequence of the antibody. Any combination of deletion, insertion and substitution can be made to arrive at the final construct, provided that the final (i.e. modified) antibody has the required properties, e.g., antigen binding.

[0135] 2.7.1 Substitution, Insertion, and Deletion Mutants In some embodiments, antibody variants with one or more amino acid substitutions are provided. Target sites for substitutional mutagenesis include HVRs (or CDRs) and FRs. Conservative substitutions are provided under the heading of "preferred substitutions" in Table 2. More substantial changes are provided under the heading of "exemplary substitutions" in Table 2 and are further described with reference to amino acid side chain classes, as follows: Amino acid substitutions can be introduced into an antibody of interest and the products screened to obtain a desired activity (e.g., preserved / improved antigen binding, reduced immunogenicity, or improved ADCC or CDC).

[0136] [Table 2]

[0137] Amino acids can be grouped according to common chain properties: (1) hydrophobic: norleucine, Met, Ala, Val, Leu, Ile, (2) neutral hydrophilic: Cys, Ser, Thr, Asn, Gln, (3) acidic: Asp, Glu, (4) alkaline: His, Lys, Arg, (5) residues that affect chain orientation: Gly, Pro, and (6) aromatic: Trp, Tyr, Phe. In some embodiments, non-conservative substitutions entail exchanging a member of one of these classes for another class.

[0138] In some embodiments, one type of substitutional variant involves the substitution of one or more hypervariable region residues of a parent antibody (e.g., a humanized or human antibody). Typically, the resulting variants are selected for further study and are modified (e.g., improved) with some biological property (e.g., increased affinity, decreased immunogenicity) relative to the parent antibody and / or essentially retain some biological property of the parent antibody. Exemplary substitutional variants are affinity matured antibodies, which are easy to generate, for example, using phage display-based affinity maturation techniques (e.g., those disclosed herein). Briefly, one or more HVR (or CDR) residues are mutated and the variant antibodies are displayed on phage and screened for a particular biological activity (e.g., binding affinity).

[0139] Modifications (e.g., substitutions) can be made in the HVRs (or CDRs) to, for example, improve antibody affinity. Such modifications can be made in HVR (or CDR) "hot points," i.e., residues encoded by codons frequently mutated during somatic maturation (see, e.g., Chowdhury, Methods Mol. Biol. 207:179-196 (2008)), and / or in the SDRs (a-CDRs), and the resulting mutant VH or VL can be tested for binding affinity. Affinity maturation by construction and reselection from a secondary library is described, for example, in Hoogenboom et al. in Methods in Molecular Biology 178:1-37 (O'Brien et al., ed., Human Press, Totowa, NJ, (2001). In some embodiments of affinity maturation, diversity is introduced into the variable genes selected for maturation by any one of a variety of methods (e.g., error-prone PCR, chain shuffling, or oligonucleotide-directed mutagenesis), producing a secondary library. The library is then screened to identify any antibody variants with the desired affinity. Another method of introducing diversity involves the HVR (or CDR) directed method, in which a number of HVR (or CDR) residues (e.g., 4-6 residues at a time) are randomized. For example, alanine scanning mutagenesis or modeling can be used to specifically identify HVR (or CDR) residues involved in antigen binding. In particular, CDR-H3 and CDR-L3 are routinely targeted.

[0140] In some embodiments, substitutions, insertions or deletions may occur within one or more HVRs (or CDRs) so long as such modifications do not substantially reduce the ability of the antibody to bind to the antigen. For example, conservative modifications (e.g., conservative substitutions according to the present specification) may be made in an HVR (or CDR) that do not substantially reduce binding affinity. Such modifications may be in HVR (or CDR) "hot points" or outside the CDR. In some embodiments of the variant VHH sequences provided above, each HVR (or CDR) is unaltered or contains no more than one, two or three amino acid substitutions.

[0141] As described in Cunningham and Wells (1989) Science, 244:1081-1085, a useful method for identifying antibody residues or regions that can undergo targeted mutagenesis is called "alanine scanning mutagenesis". In such methods, a target residue or group of residues (e.g., charged residues, e.g., Arg, Asp, His, Lys, and Glu) is identified and substituted with neutral or negatively charged amino acids (e.g., alanine or polyalanine) to determine whether the antibody-antigen interaction is affected. Additional substitutions can be introduced at the amino acid positions to demonstrate functional sensitivity to the initial substitution. Alternatively or additionally, a crystal structure of an antigen-antibody complex is used to identify contact points between the antibody and the antigen. Such contact and adjacent residues may be targeted or eliminated as substitution candidates. Mutants may be screened to determine whether they contain the desired attributes.

[0142] Amino acid sequence insertions include amino- and / or carboxyl-terminal fusions ranging in length from one residue to polypeptides containing a hundred or more residues, as well as intrasequence insertions of single or multiple amino acid residues. An example of a terminal insertion is an antibody with an N-terminal methionyl residue. Other insertional variants of the antibody molecule include the fusion to the N- or C-terminus of the antibody to an enzyme (e.g., in the case of ADEPT) or a polypeptide which increases the serum half-life of the antibody.

[0143] 2.7.2 Glycosylation variants In some embodiments, the antibody is modified to increase or decrease the degree of glycosylation of the construct. Addition or deletion of glycosylation sites to an antibody can be readily accomplished by altering the amino acid sequence to create or remove one or more glycosylation sites.

[0144] If the antibody comprises an Fc region (e.g., scFv-Fc), the carbohydrate attached thereto can be modified. Natural antibodies produced by mammalian cells usually contain branched bicontact angle oligosaccharides, which are usually linked to the Fc region C by N-linkages. H The oligosaccharide is linked to Asn297 of the .2 domain. See, e.g., Wright et al. TIBTECH 15:26-32 (1997). The oligosaccharide may contain various carbohydrates, e.g., mannose, N-acetylglucosamine (GlcNAc), galactose and sialic acid, and fucose attached to the GlcNAc in the "stem" of the bicontact angle oligosaccharide structure. In some embodiments, modifications can be made to the oligosaccharides in the antibody to produce antibody variants with some improved properties.

[0145] In some embodiments, the antibody has a carbohydrate structure that lacks fucose attached (directly or indirectly) to the Fc region. For example, the fucose content in such an antibody may be 1% to 80%, 1% to 65%, 5% to 65%, or 20% to 40%. The amount of fucose is determined by calculating the average amount of fucose in the Asn297 glycan relative to the sum of all glycostructures (e.g., complex, hybrid, and high mannose structures) attached to Asn297 as measured by MALDI-TOF mass spectrometry, e.g., as described in WO 2008 / 077546. Asn297 refers to an asparagine residue located at about 297 (EU numbering of Fc region residues) in the Fc region; however, due to minor sequence variations in antibodies, Asn297 may be located about ±3 amino acids upstream or downstream of position 297, i.e., between positions 294 and 300. Such fucosylation variants may have improved ADCC function. See, e.g., U.S. Patent Publication Nos. US2003 / 0157108 (Presta, L.), US2004 / 0093621 (Kyowa Hakko Kogyo Co., Ltd). Examples of publications relating to "defucosylated" or "fucose-deficient" antibody variants include US2003 / 0157108, WO 2000 / 61739, WO 2001 / 29246, US2003 / 0115614, US2002 / 0164328, US2004 / 0093621, US2004 / 0132140, US 2004 / 0110704, US2004 / 0110282, US2004 / 0109865, WO 2003 / 085119, WO 2003 / 084570, WO 2005 / 035586, WO 2005 / 035778, WO2005 / 053742, WO2002 / 031140, Okazaki et al. J. Mol. Biol. 336:1239-1249 (2004), Yamane-Ohnuki et al. Biotech. Bioeng. 87: 614 (2004).Examples of cell lines capable of producing defucosylated antibodies include protein fucosylation-deficient Lec13 CHO cells (Ripka et al. Arch. Biochem. Biophys. 249:533-545 (1986); U.S. Patent Application No. US2003 / 0157108 A1, Presta, L, and WO 2004 / 056312 A1, Adams et al.), and knockout cell lines, such as α-1,6-fucosyltransferase gene FUT8, knockout CHO cells (see, e.g., Yamane-Ohnuki et al. Biotech. Bioeng. 87: 614 (2004); Kanda, Y. et al., Biotechnol. Bioeng., 94(4):680-688 (2006), and WO2003 / 085107).

[0146] In some embodiments, the antibody has a bisected oligosaccharide, for example, where the bi-contact angle oligosaccharide attached to the Fc region of the antibody is bisected by GlcNAc. Such antibody variants may have reduced fucosylation and / or improved ADCC function. Examples of such antibody variants are described, for example, in WO 2003 / 011878 (Jean-Mairet et al.), U.S. Patent No. 6,602,684 (Umana et al.), and US2005 / 0123546 (Umana et al.). Further provided is an antibody variant having at least one galactose residue linked to the Fc region in the oligosaccharide. Such antibody variants may have improved CDC function. Such antibody variants are described, for example, in WO 1997 / 30087 (Patel et al.), WO 1998 / 58964 (Raju, S.), and WO 1999 / 22764 (Raju, S.).

[0147] 2.7.3 Fc Region Variants In some embodiments, the Fc region of an antibody or antibody derivative of the invention may comprise a human Fc region sequence (e.g., a human IgG1, IgG2, IgG3, or IgG4 Fc region), which comprises an amino acid modification (e.g., a substitution) at one or more amino acid positions. In some embodiments, an Fc region variant can be generated by introducing one or more amino acid modifications into the Fc region of an antibody moiety (e.g., an IgG scFv-Fc or VHH-Fc).

[0148] In some embodiments, the Fc region has some (but not all) effector functions that make it a desirable candidate for applications in which the half-life of the antibody in vivo is important, but some effector functions (e.g., complement and ADCC) are not necessary or are deleterious. In vitro and / or in vivo cytotoxicity assays can be performed to confirm the reduction / depletion of CDC and / or ADCC activity. For example, Fc receptor (FcR) binding assays can be performed to ensure that the antibody does not have FcγR binding capacity (and thus may lack ADCC activity) but can retain FcRn binding capacity. Primary cells for mediating ADCC, NK cells, express only FcγRIII, whereas monocytes express FcγRI, FcγRII, and FcγRIII. FcR expression on hematopoietic cells is summarized in Ravetch and Kinet, Annu. Rev. Immunol. 9:457-492 (1991). Non-limiting examples of in vitro assays for assessing ADCC activity of a molecule of interest are described in U.S. Patent Nos. 5,500,362 (see, e.g., Hellstrom, I. et al. Proc. Nat'l Acad. Sci. USA 83:7059-7063 (1986) and Hellstrom, I et al., Proc. Nat'l Acad. Sci. USA 82:1499-1502 (1985)), 5,821,337 (see, Bruggemann, M. et al., J. Exp. Med. 166:1351-1361 (1987)). Alternatively, non-radioactive assay methods (e.g., ACTI for flow cytometry) can be used. TMNon-radioactive cytotoxicity assays (see CellTechnology, Inc. Mountain View, CA, and CytoTox 96® non-radioactive cytotoxicity assay (Promega, Madison, WI) may be employed. Useful effector cells for such assays include peripheral blood mononuclear cells (PBMC) and natural killer (NK) cells. Alternatively, or additionally, ADCC activity of the molecule of interest may be assessed in vivo (e.g., in an animal model), e.g., as disclosed in Clynes et al. Proc. Nat'l Acad. Sci. USA 95:652-656 (1998). C1q binding assays can be performed to confirm that the antibody is unable to bind C1q, and therefore lacks CDC activity. See, e.g., WO 2006 / 029879 and WO See C1q and C3c binding to ELISA in 2005 / 100402. To assess complement activation, CDC assays may be performed (see, e.g., Gazzano-Santoro et al., J. Immunol. Methods 202:163 (1996); Cragg, MS et al., Blood 101:1045-1052 (2003); and Cragg, MS and MJ Glennie, Blood 103:2738-2743 (2004)). FcRn binding and in vivo clearance / half-life assays may be performed using methods known in the art (see, e.g., Petkova, SB et al., Int'l. Immunol. 18(12):1759-1769 (2006)).

[0149] Antibodies with reduced effector function include those antibodies with one or more substitutions at Fc region residues 238, 265, 269, 270, 297, 327 and 329 (U.S. Patent No. 6,737,056). Such Fc mutants include Fc mutants with substitutions of two or more at amino acid positions 265, 269, 270, 297 and 327, including the so-called "DANA" Fc mutant in which residues 265 and 297 are replaced by alanine (U.S. Patent No. 7,332,581).

[0150] Several antibody variants with improved or reduced binding to FcRs have been described (see, e.g., U.S. Pat. No. 6,737,056, WO 2004 / 056312, and Shields et al., J. Biol. Chem. 9(2): 6591-6604 (2001)).

[0151] In some embodiments, the Fc region comprises one or more mutations based on EU numbering of residues. In some embodiments, the Fc region is an IgG1 Fc region. In some embodiments, the Fc region is an IgG2 or IgG4 Fc region.

[0152] In some embodiments, the IgG1 or IgG4 Fc region comprises one or more mutations that modify effector function. In some embodiments, the IgG1 Fc region comprises a L234A mutation and / or a L235A mutation. In some embodiments, the IgG4 Fc region comprises a F234A and / or a L235A mutation. In some embodiments, the Fc region comprises a substitution at position 297, e.g., N297A, N297Q, or N297G.

[0153] In some embodiments, the IgG1 Fc region comprises one or more mutations that modify antibody-dependent cell-mediated cytotoxicity (ADCC). In some embodiments, the IgG1 Fc region comprises a mutation that reduces antibody-dependent cell-mediated cytotoxicity (ADCC). In some embodiments, the IgG1 Fc region comprises a mutation that enhances antibody-dependent cell-mediated cytotoxicity (ADCC). In some embodiments, the IgG1 Fc region comprises L235V, F243L, R292P, Y300L, and P396L mutations. In some embodiments, the IgG1 Fc region comprises S239D, A330L, and I332E mutations. In some embodiments, the IgG1 Fc region comprises L235V, F243L, R292P, and Y300L mutations. In some embodiments, the IgG1 Fc region comprises substitutions at positions 298, 333 and / or 334 of the Fc region, e.g., S298A, E333A and K334A.

[0154] In some embodiments, the Fc region comprises one or more mutations that alter covalent binding to an Fc receptor (e.g., FcγRIIa and / or FcγRIIb). In some embodiments, the Fc region comprises one or more mutations that enhance covalent binding to an Fc receptor. In some embodiments, the Fc region comprises a mutation that enhances covalent binding to FcγRIIa, FcγRIIb, or a combination thereof. In some embodiments, the Fc region comprises S267E and L328F mutations. In some embodiments, the Fc region comprises N325S and L328F mutations.

[0155] In some embodiments, the Fc region comprises an IgG4 Fc region comprising an S228P mutation.

[0156] In some embodiments, the Fc region comprises knob-in-hole mutations, whereby two different antibody chains form heterodimers to produce a multispecific antibody. In some embodiments, the knob-in-hole mutations comprised in the multispecific antibodies disclosed herein are selected from T366S, L368A, T366W, Y349C, Y407V, S354C, and any combination thereof. In some embodiments, the multispecific antibody comprises knob-in-hole mutations of T366S and L368A in the hole chain and T366W in the knob chain. In some embodiments, the multispecific antibody comprises knob-in-hole mutations of T366S, L368A, and Y407V in the hole chain and T366W in the knob chain. In some embodiments, the multispecific antibody comprises knob-hole structure mutations of Y349C, T366S, L368A and Y407V in the hole chain and S354C and T366W in the knob chain.

[0157] In some embodiments, modifications are made in the Fc region that result in modified (i.e., enhanced or decreased) C1q binding and / or complement dependent cytotoxicity (CDC), e.g., as described in U.S. Pat. No. 6,194,551, WO 1999 / 51642, and Idusogie et al. J. Immunol. 164: 4178-4184 (2000).

[0158] In some embodiments, the antibody (e.g., scFv-Fc or VHH-Fc) variant comprises a variant Fc region that contains one or more amino acid substitutions that alter the half-life and / or alter binding to the neonatal Fc receptor (FcRn). US2005 / 0014934A1 (Hinton et al.) describes antibodies with extended half-life and improved binding to the neonatal Fc receptor (FcRn), which is responsible for the transfer of maternal IgG to the fetus (Guyer et al., J. Immunol. 117:587 (1976) and Kim et al., J. Immunol. 24:249 (1994). These antibodies include an Fc region with one or more substitutions that alter the binding of the Fc region to FcRn. Such Fc variants include Fc variants with substitutions at one or more Fc region residues, for example, substitution of Fc region residue 434 (U.S. Pat. No. 7,371,826). In some embodiments, the Fc region includes M428L and N434S mutations. In some embodiments, the Fc region includes M252Y, S254T, and T256E mutations.

[0159] In some embodiments, the Fc region comprises an Fc region variant described in Duncan & Winter, Nature 322:738-40 (1988); Wang et al., Protein Cell 2018,9(1):63-73; U.S. Patent No. 5,648,260; U.S. Patent No. 5,624,821; and WO 1994 / 29351.

[0160] 2.7.4 Cysteine ​​Engineered Antibody Variants In some embodiments, it may involve the production of cysteine ​​engineered antibody moieties, e.g., "thioMAbs," in which one or more residues of an antibody are replaced by cysteine ​​residues. In some embodiments, the substituted residues are located at accessible sites of the antibody. By replacing these residues with cysteine ​​residues, reactive thiol groups are positioned at accessible sites of the antibody and may be used to conjugate the antibody to other moieties, e.g., drug moieties or linker-drug moieties, to produce immunoconjugates, e.g., as further described herein. In some embodiments, any one or more residues of A118 (EU numbering) of the heavy chain and S400 (EU numbering) of the heavy chain Fc region may be replaced by cysteine. Cysteine ​​engineered antibody moieties may be generated, for example, in the manner described in U.S. Pat. No. 7,521,541.

[0161] 2.8 Antibody derivatives In some embodiments, the antibodies described herein may be further modified into antibody derivatives containing other proteins or non-protein moieties known in the art and readily available. Non-protein moieties suitable for antibody derivatization include, but are not limited to, water-soluble polymers. Non-limiting examples of water-soluble polymers include, but are not limited to, polyethylene glycol (PEG), ethylene glycol / propylene glycol copolymers, carboxymethylcellulose, dextran, polyvinyl alcohol, polyvinylpyrrolidone, poly 1,3-dioxolane, poly 1,3,6-trioxane, ethylene / maleic anhydride copolymers, polyamino acids (homopolymers or random copolymers), dextran or poly(n-vinylpyrrolidone) polyethylene glycol, propylene glycol homopolymer, polypropylene oxide / ethylene oxide copolymer, polyoxyethylated polyols (e.g., glycerol), polyvinyl alcohol, and mixtures thereof. Polyethylene glycol propionaldehyde may have advantages in preparations due to its stability in water. The polymers may have any molecular weight and may be branched or unbranched. The number of polymers attached to an antibody can vary, and when more than one type of polymer is attached, they can be the same or different molecules. Generally, the number and / or type of polymers used for derivatization can be determined based on several considerations, including, but not limited to, the particular property or function of the antibody to be improved, whether the antibody derivative will be used for diagnostic purposes under defined conditions, etc.

[0162] In some embodiments, the antibody may be further modified into an antibody derivative that includes one or more biologically active proteins, polypeptides, or fragments thereof. As used interchangeably herein, "biologically active" or "having biological activity" refers to a biological activity exhibited in vivo to perform a specific function, and may mean, for example, binding to a specific biological molecule (e.g., protein, DNA, etc.) and promoting or inhibiting the activity of such a biological molecule. In some embodiments, biologically active proteins or fragments thereof include proteins and polypeptides administered to a patient as active drug substances, proteins and polypeptides used for the prevention or treatment and diagnosis of a disease or condition (e.g., enzymes used in diagnostic tests or in vitro assays), and proteins and polypeptides administered to a patient to prevent a disease (e.g., vaccines).

[0163] 2.9 Production Method The antibodies and antibody derivatives disclosed herein can be produced using any available or known technique in the art. For example, but not limited to, the antibodies and antibody derivatives can be produced using recombinant methods and compositions, such as those described in U.S. Patent No. 4,816,567. Detailed procedures for producing the antibodies and antibody derivatives are described in detail in the examples below.

[0164] The present subject matter further provides an isolated nucleic acid encoding an antibody or antibody derivative disclosed herein. For example, the isolated nucleic acid can encode an amino acid sequence comprising the VL and / or an amino acid sequence comprising the VH of the antibody, e.g., the light chain and / or the heavy chain of the antibody.

[0165] In some embodiments, the nucleic acid may be present in one or more vectors (e.g., expression vectors). As used herein, the term "vector" refers to a nucleic acid molecule capable of transporting another nucleic acid linked to it. One type of vector is a "plasmid," which refers to a circular double-stranded DNA loop to which another DNA segment can be linked. Another type of vector is a viral vector, in which another DNA segment can be linked to the viral genome. Some vectors are capable of autonomous replication in a host cell into which they are introduced (e.g., bacterial vectors and episomal mammalian vectors having a bacterial origin of replication). Other vectors (e.g., non-episomal mammalian vectors) are integrated into the genome of the host cell after being introduced into the host cell, and thereby replicate along with the host genome. Also, some vectors expression vectors can direct the expression of genes to which they are operably linked. Generally, expression vectors used in recombinant DNA techniques are always in the form of plasmids (vectors). However, the disclosed subject matter is intended to include other expression vectors having equivalent functions, such as viral vectors (e.g., replication defective retroviruses, adenoviruses and adeno-associated viruses).

[0166] The different parts of the antibody or antibody derivative disclosed herein may be constructed in a single polycistronic expression cassette, multiple expression cassettes in a single vector, or multiple vectors. Examples of elements generating polycistronic expression cassettes include, but are not limited to, various viral and non-viral internal ribosome entry sites (IRES, e.g., FGF-1 IRES, FGF-2 IRES, VEGF IRES, IGF-II IRES, NF-kB IRES, RUNX1 IRES, p53 IRES, Hepatitis A IRES, Hepatitis C IRES, Pestivirus IRES, Foot and Mouth Disease Virus IRES, Picornanilus IRES, Poliovirus IRES, and Encephalomyocarditis Virus IRES), and cleavable linkers (e.g., 2A peptides, e.g., P2A, T2A, E2A, and F2A peptides). Also suitable is the combination of a retroviral vector with an appropriate packaging thread, where the capsid protein is capable of infecting human cells. Cell lines producing various amphipathic viruses are known and include, but are not limited to, PA12 (Miller, et al. (1985) Mol. Cell. Biol. 5:431-437), PA317 (Miller, et al. (1986) Mol. Cell. Biol. 6:2895-2902), and CRIP (Danos, et al. (1988) Proc. Natl. Acad. Sci. USA 85:6460-6464). Non-amphipathic particles are also suitable, for example, using VSVG, RD114 or GALV envelopes and any other pseudotyped particles known in the art.

[0167] In some embodiments, a nucleic acid encoding an antibody or antibody derivative of the invention and / or one or more vectors comprising the nucleic acid can be introduced into a host cell. In some embodiments, the nucleic acid can be introduced into a cell by any method known in the art, including, but not limited to, transfection, electroporation, microinjection, infection with a viral or phage vector comprising the nucleic acid sequence, cell fusion, chromosome-mediated gene transfer, microcell-mediated gene transfer, spheroplast fusion, and the like. In some embodiments, the host cell can include, for example, a host cell transformed with a vector comprising a nucleic acid, the nucleic acid encoding a single domain antibody and / or an amino acid sequence comprising the VH of the single domain antibody. In some embodiments, the host cell can include, for example, a host cell transformed with (1) a vector comprising a nucleic acid encoding an amino acid sequence comprising the VL of the antibody and an amino acid sequence comprising the VH of the antibody, or (2) a first vector comprising a nucleic acid encoding an amino acid sequence comprising the VL of the antibody and a second vector comprising a nucleic acid encoding an amino acid sequence comprising the VH of the antibody. In some embodiments, the host cell is a eukaryotic cell, such as a Chinese hamster ovary (CHO) cell or a lymphocyte (eg, YO, NSO, Sp20 cell).

[0168] In some embodiments, methods for preparing an antibody or antibody derivative disclosed herein may include culturing host cells (into which nucleic acid encoding the antibody or antibody derivative has been introduced) under conditions suitable for antibody or antibody derivative expression, and optionally recovering the antibody or antibody derivative from the host cells and / or host cell medium. In some embodiments, the antibody or antibody derivative is recovered from the host cells by chromatographic techniques.

[0169] To recombinantly produce the antibody or antibody derivative of the present invention, for example, the nucleic acid encoding the antibody or antibody derivative described above can be isolated and inserted into one or more vectors for further cloning and / or expression in a host cell. These nucleic acids can be easily isolated and sequenced using conventional procedures (e.g., by using oligonucleotide probes capable of specifically binding to genes encoding the heavy and light chains of the antibody or antibody derivative). Suitable host cells for cloning or expressing the antibody-encoding vector include prokaryotic or eukaryotic cells as described herein. For example, the antibody or antibody derivative may be produced in bacteria, especially when fucosylation and Fc effector functions are not required. For expression of antibody fragments and polypeptides in bacteria, see, for example, U.S. Patent Nos. 5,648,237, 5,789,199 and 5,840,523. (See further Charlton, Methods in Molecular Biology, Vol. 248 (BKC Lo, ed., Humana Press, Totowa, NJ, 2003), pp. 245-254, which describes expression of antibody fragments in E. coli.) After expression, the antibody or antibody derivative may be isolated as a soluble fraction from the bacterial cell paste and further purified.

[0170] In addition to prokaryotes, eukaryotic microbes (e.g., filamentous fungi or yeast) are also suitable cloning or expression hosts for antibody-encoding vectors, including fungal and yeast strains in which the glycosylation pathway has been "humanized" to produce antibodies or antibody derivatives with partial or complete human glycosylation patterns. See Gemgross, Nat. Biotech. 22:1409-1414 (2004), and Li et al., Nat. Biotech. 24:21 0-215 (2006). Suitable host cells for expressing glycosylated antibodies may be derived from multicellular organisms (invertebrates and vertebrates). Examples of invertebrate cells include plants and insect cells. Many baculovirus strains have been identified that can be used in combination with insect cells, particularly for transfection of Spodoptera frugiperda cells. In some embodiments, plant cell cultures may be used as host cells. See, e.g., U.S. Patent Nos. 5,959,177, 6,040,498, 6,420,548, 7,125,978 and 6,417,429 (PLANTIBODIES PRODUCING ANTIBODIES IN TRANSGENIC PLANTS). TM (where the technology is described)

[0171] In some embodiments, vertebrate cells may be used as host cells, for example but not limited to, mammalian cell lines adapted to suspension growth may be useful. Non-limiting examples of useful mammalian host cell lines include monkey kidney CV1 line (COS-7) transformed with SY40, human embryonic kidney line (293 or 293 cells, e.g., as described in Graham et al., J Gen Viral. 36:59 (1977)), baby hamster kidney cells (BHK), mouse sertoli cells (TM4 cells, e.g., as described in Mather, Biol. Reprod. 23:243-251 (1980)), monkey kidney cells (CV 1); African green monkey kidney cells (VERO-76), human cervical carcinoma cells (HELA), canine kidney cells (MDCK, buffalo rat liver cells (BRL 3A), human lung cells (W138), human liver cells (Hep 02), mouse mammary tumor (MMT 060562), e.g., as described in Mather et al., Annals NY Acad. Sci. 383:44-68 (1982), TRI cells, MRC 5 cells, and FS4 cells. Other useful mammalian host cell lines include Chinese hamster ovary (CHO) cells, including DHFK CHO cells (Urlaub et al., Proc. Natl. Acad. Sci. USA 77:42 I6 (1980)) and myeloma cell lines (e.g., YO, NSO, and Sp2 / 0). For a review of several mammalian host cell lines suitable for the production of antibodies or antibody derivatives, see, e.g., Yazaki and Wu, Methods in Molecular Biology, Vol. 248 (BKC Lo, ed., Humana Press, Totowa, NJ), pp. 255-268 (2003).

[0172] In some embodiments, techniques for preparing bispecific and / or multispecific antibodies include, but are not limited to, recombinant expression of two immunoglobulin heavy chain light chain pairs with the same specificity, where one or both heavy or light chains are fused to an antigen binding moiety with a different specificity (e.g., a single domain antibody, e.g., a VHH), recombinant co-expression of two immunoglobulin heavy chain light chain pairs with different specificities (see Milstein and Cuello, Nature 305: 537 (1983), PCT Patent Application No. WO 93 / 08829 and Traunecker et al., EMBO J 10: 3655 (1991)), and "knob-hole" engineering (see, e.g., U.S. Pat. No. 5,731,168). Bispecific antibodies may also be prepared by engineering electrostatic steering effects to prepare antibody Fc-heterodimeric molecules (WO 2009 / 089004A1), cross-linking two or more antibodies or fragments (see, e.g., U.S. Pat. No. 4,676,980 and Brennan et al., Science, 229: 81 (1985)), using leucine zippers to produce bispecific antibodies (see, e.g., Kostelny et al., J Immunol., 148(5): 1547-1553 (1992)), using "diabody" technology to prepare bispecific antibody fragments (see, e.g., Hollinger et al., Proc. Natl. Acad. Sci. USA, 90:6444-6448 (1993)), and using single-chain Fv (sFv) dimers (see, e.g., Gruber et al., J. Immunol., 152:5368 (1994)), and trispecific antibodies can be prepared, e.g., as described in Tutt et al. J Immunol. 147: 60 (1991).

[0173] Bispecific and multispecific molecules of the invention may be prepared by chemical techniques (see, e.g., Kranz (1981) Proc. Natl. Acad. Sci. USA 78:5807), "polydoma" techniques (e.g., U.S. Pat. No. 4,474,893), or recombinant DNA techniques. Additionally, bispecific and multispecific molecules of the presently disclosed subject matter may be prepared by conjugating constituent binding specificities, e.g., a first epitope and a second epitope binding specificity, by methods known in the art and described herein. For example, but not limited to, each binding specificity of the bispecific and multispecific molecule may be prepared together or separately and then conjugated to each other by recombinant fusion protein technology. When the binding specificities are proteins or peptides, covalent attachment may be performed using a variety of coupling or cross-linking agents. Non-limiting examples of cross-linking agents include protein A, carbodiimide, N-succinimidyl-S-acetyl-thioacetate (SATA), N-succinimidyl-3-(2-pyridyldithio)propionate (SPDP), and sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate (sulfo-SMCC) (see, e.g., Karpovsky (1984) J. Exp. Med. 160:1686; Liu (1985) Proc. Natl. Acad. Sci. USA 82:8648). Other methods include those described by Paulus (Behring Ins. Mitt. (1985) No. 78,118-132; Brennan (1985) Science 229:81-83); Glennie (1987) J Immunol. 139: 2367-2375). When the binding specificities are antibodies (e.g., two humanized antibodies), they may be conjugated via sulfhydryl bonds in the C-terminal hinge regions of the two heavy chains. In some embodiments, prior to conjugation, the hinge region may be modified to contain an odd number of sulfhydryl residues (e.g., one).

[0174] In some embodiments, the two binding specificities of a bispecific antibody may be encoded in the same vector and expressed and assembled in the same host cell. The method is particularly useful when the bispecific and multispecific molecules are MAb x MAb, MAb x Fab, Fab x F(ab')2 or Ligand x Fab fusion proteins. In some embodiments, the bispecific antibodies of the invention may be single chain molecules, e.g., single chain bispecific antibodies, single chain bispecific molecules comprising one single chain antibody and a binding determinant cluster, or single chain bispecific molecules comprising two binding determinant clusters. Bispecific and multispecific molecules may be single chain molecules or may comprise at least two single chain molecules. Methods for preparing bispecific and multispecific molecules are described, for example, in U.S. Patent No. 5,260,203, U.S. Patent No. 5,455,030, U.S. Patent No. 4,881,175, U.S. Patent No. 5,132,405, U.S. Patent No. 5,091,513, U.S. Patent No. 5,476,786, U.S. Patent No. 5,013,653, U.S. Patent No. 5,258,498, and U.S. Patent No. 5,482,858. This specification further includes engineered antibodies having three or more functional antigen binding sites (e.g., epitope binding sites), including "octopus antibodies" (see, e.g., US 2006 / 0025576 A1).

[0175] In some embodiments, an animal system can be used to produce the antibodies or antibody derivatives of the invention. The animal system for preparing hybridomas is the murine system.

[0176] In the mouse, the production of hybridomas is a well-established procedure. Immunization protocols and techniques for isolating immunized splenocytes for fusion are known in the art. Fusion partners (e.g., mouse myeloma cells) and fusion procedures are also known (see, e.g., Harlow and Lane (1988), Antibodies, A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor New York).

[0177] 2.10 Assay The antibodies and antibody derivatives of the invention herein can be identified, screened or characterized for their physical / chemical properties and / or biological activity by a number of assays known in the art and as described herein.

[0178] In some embodiments, the antigen-binding activity of the antibody or antibody derivative of the invention can be tested by known methods (e.g., enzyme-linked immunosorbent assay (ELISA), radioimmunoassay (RIA) or Western blot assay). Each of these assays detects the presence of a protein-antibody complex of particular interest, usually by a labeled reagent (e.g., an antibody) having specificity for the complex of interest. For example, an antibody or antibody derivative can be detected by, for example, an enzyme-linked antibody or antibody fragment that recognizes and specifically binds to the antibody or antibody derivative. Alternatively, the antibody or antibody derivative can be detected by any one of several other immunoassays. For example, the antibody or antibody derivative can be radiolabeled and used in a radioimmunoassay (RIA) (see, e.g., Weintraub, B., Principles of Radioimmunoassays, Seventh Training Course on Radioligand Assay Techniques, The Endocrine Society, March 1986, which is incorporated herein by reference). For example, radioisotopes can be detected by means such as a Geiger counter or a scintillation counter or by autoradiography.

[0179] In some embodiments, competitive assays may be used to identify antibodies or antibody derivatives that compete with the antibodies of the invention for binding to OX40. In some embodiments, such competing antibodies bind to the same epitope (e.g., linear or conformational epitope) that the antibodies disclosed herein bind. Detailed exemplary methods for locating epitopes that bind to antibodies are provided in Morris (1996) "Epitope Mapping Protocols", Methods in Molecular Biology, vol. 66 (Humana Press, Totowa, NJ).

[0180] In a non-limiting example of a competitive assay, immobilized OX40 can be incubated in a solution containing a first labeled antibody or antibody derivative that binds to OX40 and a second unlabeled antibody, and the ability of the second unlabeled antibody to compete with the first antibody for binding to OX40 can be tested. The second antibody can be present in a hybridoma supernatant. As a control, immobilized OX40 is incubated in a solution containing the first labeled antibody but not the second unlabeled antibody. After incubation under conditions that allow binding of the first antibody to OX40, excess unbound antibody is removed and the amount of label associated with immobilized OX40 is measured. A significant decrease in the amount of label associated with immobilized OX40 in the test sample compared to the control sample indicates that the second antibody competes with the first antibody for binding to OX40. See Harlow and Lane (1988) Antibodies: A Laboratory Manual ch.14 (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY).

[0181] The present invention provides assays for identifying antibodies or antibody derivatives thereof that have biological activity. Biological activity may include, for example, activation of immune cells or immune activation reporter genes (e.g., NFAT reporter genes or NF-κB reporter genes). Antibodies that have such biological activity in vivo and / or in vivo are further provided.

[0182] 2.11 Immunoconjugates The present subject matter further provides immunoconjugates, which include an antibody or antibody derivative disclosed herein conjugated to one or more detection probes and / or a cytotoxic agent (e.g., a chemotherapeutic agent or drug, a growth inhibitory agent, a toxin (e.g., a protein toxin, an enzymatically active toxin of bacterial, fungal, plant or animal origin, or fragments thereof), or a radioisotope. For example, an antibody or antigen-binding portion of the disclosed subject matter may be operatively linked (e.g., by chemical coupling, genetic fusion, non-covalent association or other modes) to one or more other binding molecules, e.g., another antibody, an antibody fragment, a peptide, or a binding mimetic.

[0183] In some embodiments, the immunoconjugate is an antibody-drug conjugate (ADC), in which the antibody is conjugated to one or more drugs, such as maytansinoids (U.S. Pat. Nos. 5,208,020, 5,416,064, and EP 1 366 636). 5,770,701, 5,770,710, 5,773,001, and 5,877,296; Hinman et al., Cancer Res. 53:3336-3342 (1993); and Lode et al., Cancer Res. 53:3336-3342 (1994); and U.S. Pat. Nos. 5,635,483, 5,780,588, and 7,498,298; dolastatins, calicheamicin or derivatives thereof (see U.S. Pat. Nos. 5,712,374, 5,714,586, 5,739,116, 5,767,285, 5,770,701, 5,770,710, 5,773,001, and 5,877,296; Hinman et al., Cancer Res. 53:3336-3342 (1993); and Lode et al., Cancer Res. 53:3336-3342 (1994); and U.S. Pat. Res. 58:2925-2928 (1998)), anthracyclines such as daunomycin or doxorubicin (Kratz et al., Current Med Chem. 13:477-523 (2006); Jeffrey et al., Bioorganic & Med. Chem. Letters 16:358-362 (2006); Torgov et al., Bioconj. Chem. 16:717-721 (2005); Nagy et al., Proc. Natl. Acad. Sci. USA 97:829-834 (2000); Dubowchik et al., Bioorg. & Med. Chem. Letters 12:1529-1532 (2002); King et al., J. Med. Chem.45:4336-4343 (2002), and U.S. Patent No. 6,630,579), methotrexate, vindesine, taxanes such as docetaxel, paclitaxel, larotaxel, tesetaxel, and ortaxel, trichothecenes, and CC1065.

[0184] In some embodiments, an immunoconjugate comprises an antibody described herein conjugated to an enzymatically active toxin or fragment thereof, including, but not limited to, diphtheria A chain, nonbinding active fragments of diphtheria toxin, exotoxin A chain (from Pseudomonas aeruginosa), ricin A chain, abrin A chain, modeccin A chain, α-swellin, abrin, dianthin, Phytolaca americana (PAPI, PAPII, and PAP-S), momordica charantia inhibitor, jatrosin, crotin, sapaonaria officinalis inhibitor, gelonin, mitogellin, restrictocin, phenomycin, enomycin, and the trichothecenes.

[0185] In some embodiments, the immunoconjugate comprises an antibody as described herein conjugated to a radioactive atom to form a radioconjugate. Multiple radioisotopes may be used in the production of radioconjugates. Non-limiting examples include At 211 , I 131 , I 125 , Y 90 , Re 186 , Re 188 , Sm 153 , Bi 212 , P 32 , Pb 212and radioactive isotopes of Lu. When a radioactive conjugate is used for detection, it may include radioactive atoms used in scintillation studies, such as tc99m or 1123, or spin labels used in nuclear magnetic resonance (NMR) imaging (also called magnetic resonance imaging, MRI), such as iodine-123, iodine-131, indium-11, fluorine-19, carbon-13, nitrogen-15, oxygen-17, gadolinium, manganese, or iron.

[0186] A variety of bifunctional protein coupling agents (e.g., N-succinimidyl-3-(2-pyridinedimercapto)propionate (SPDP), succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (SMCC), iminosulfan (IT), bifunctional derivatives of imidoesters (e.g., dimethyladipimidate HCl), active esters (e.g., disuccinimidyl suberate), aldehydes (e.g., glutaraldehyde), disazo compounds (e.g., bis(p-azidobenzoyl)hexanediamine), double nitrogen derivatives (e.g., bis-(p-diazobenzoyl)-ethylenediamine), diisocyanates (e.g., tolylene 2,6-diisocyanate), and bis-active fluorine compounds (e.g., 1,5-difluoro-2,4-dinitrobenzene)) can be used to prepare conjugates of antibodies and cytotoxic agents. For example, ricin immunotoxins can be prepared as described in Vitetta et al., Science 238: 1098 (1987). Carbon 4-labeled l-isothiocyanatobenzyl-3-methyldiethylenetriamine-pentaacetic acid (MX-DTPA) is an exemplary chelating agent for conjugating radionucleotides to antibodies. See WO 94 / 11026. The linker may be a "cleavable linker" that facilitates the release of the cytotoxic drug in cells. For example, acid-labile linkers, peptidase-sensitive linkers, photolabile linkers, dimethyl linkers, or disulfide-containing linkers can be used (Chari et al., Cancer Res. 52:127-1 31 (1992), U.S. Patent No. 5,208,020).

[0187] Immunoconjugates or ADCs herein expressly cover such conjugates prepared using crosslinkers, including, but not limited to, BMPS, EMCS, GMBS, HBVS, LC-SMCC, MBS, MPBH, SBAP, SIA, SIAB, SMCC, SMPB, SMPH, sulfo-EMCS, sulfo-GMBS, sulfo-KMUS, sulfo-MBS, sulfo-SIAB, sulfo-SMCC, and sulfo-SMPB, and SVSB (Succinimidyl-(4-vinylsulfone) vinylsulfone) (e.g., from Pierce Biotechnology, Inc., Rockford, Ill., USA).

[0188] 2.12 Antigen Recognition Receptor The subject matter of the present invention further provides an antigen-recognizing receptor comprising the antibody or antibody fragment disclosed herein.Antigen-recognizing receptors are receptors that can activate, stimulate or inhibit immune-responsive cells (e.g., T cells) in response to their binding to an antigen.Non-limiting examples of antigen-recognizing receptors include natural and recombinant T cell receptors ("TCRs"), chimeric costimulatory receptors (CCRs), chimeric antigen receptors ("CARs") or inhibitory CARs (iCARs). The design and use of antigen-recognizing receptors are well known in the art and have been described in the literature, for example, International Publication Nos. WO 2018 / 027155, WO 2019 / 099483, WO 2019 / 157454, WO 2019 / 133969, WO 2019 / 099993, WO 2015 / 142314, WO 2018 / 027197, and WO 2014055668.

[0189] In some embodiments, the subject matter provides chimeric antigen receptors (CARs) comprising the antibodies, antibody fragments, or multispecific antibodies disclosed herein. CARs are engineered receptors that can graft or confer a desired specificity onto immune effector cells. In some embodiments, CARs can be used to graft the specificity of a monoclonal antibody onto T cells, facilitating the transfer of its coding sequence via a vector. In some embodiments, the CAR is a "first generation" CAR, which is typically composed of an extracellular antigen binding domain (e.g., scFav or VHH) fused to a transmembrane domain, which is fused to a cytoplasmic / intracellular signaling domain. "First generation" CARs provide de novo antigen recognition regardless of HLA-mediated antigen presentation and can trigger activation of immune responsive cells (e.g., CD4+ and CD8+ T cells) via their CD3z chain signaling domain in a single fusion molecule. In some embodiments, the CAR is a "second generation" CAR, which further comprises intracellular signaling domains from various costimulatory molecules (e.g., CD28, 4-1BB, ICOS, OX40, CD27, CD40 / My88, and NKGD2) to the cytoplasmic tail region of the CAR to provide additional signals to the immunoresponsive cell, whereby "second generation" CARs include those that provide both costimulation (e.g., CD28 or 4-1BB) and activation (CD3z). In some embodiments, the CAR is a "third generation" CAR, which comprises multiple costimulatory domains (e.g., CD28 and 4-1BB) and activation (CD3z). In some embodiments, the CAR is a second generation CAR. In some embodiments, the CAR comprises an extracellular antigen binding domain that binds to an antigen, a transmembrane domain, and an intracellular signaling domain, where the intracellular signaling domain comprises a costimulatory signaling domain. In some embodiments, the CAR further comprises a hinge / spacing region between the extracellular antigen binding domain and the transmembrane domain. In some embodiments, the extracellular antigen-binding domain comprises an antibody, antibody fragment, or multispecific antibody disclosed herein.In some embodiments, the antibody, antibody fragment, or multispecific antibody comprises a VHH, Fab, or scFv. In some embodiments, the CAR comprises a multispecific antibody disclosed herein.

[0190] In some embodiments, the subject invention provides a recombinant TCR comprising an antibody or antibody fragment disclosed herein. A native TCR is a protein complex comprising a heterodimeric protein linked by disulfide bonds, the heterodimeric protein being composed of two variable chains expressed as part of a complex with a CD3 chain molecule. A native TCR is present on the surface of a T cell and is responsible for recognizing antigens as peptides that bind to major histocompatibility complex (MHC) molecules. In some embodiments, a native TCR comprises an α chain and a β chain (encoded by the TRA and TRB genes, respectively). In some embodiments, a TCR comprises a γ chain and a δ chain (encoded by the TRG and TRD genes, respectively). Each of the α, β, γ and δ chains comprises two extracellular domains, a variable (V) region and a constant (C) region. The constant region is close to the cell membrane and is followed by a transmembrane region and a short cytoplasmic tail region. The variable region binds to the peptide / MHC complex. Each variable region comprises three complementarity determining regions (CDRs). In some embodiments, the TCR comprises a receptor complex with CD3δ, CD3γ, CD3ε and CD3ζ. A T cell expressing the TCR complex is activated when the TCR complex binds to its antigen and MHC (peptide / MHC).

[0191] In some embodiments, the recombinant TCR is a non-naturally occurring TCR. In some embodiments, the recombinant TCR comprises a recombinant alpha chain and / or a recombinant b chain, where a portion or the entire variable region of the recombinant alpha chain and / or the recombinant b chain is replaced with an antibody or antibody fragment disclosed herein. In some embodiments, the antibody or antibody fragment comprises a VHH, VH, VL, Fab or scFv. In some embodiments, the antibody or antibody fragment comprises a VHH. In some embodiments, the recombinant TCR binds to an antigen of interest in an MHC / HLA-independent manner. In some non-limiting embodiments, antigen binding can activate an immunoresponsive cell comprising the recombinant TCR.

[0192] The present subject matter provides an immunoresponsive cell, which comprises (a) an antigen recognition receptor (e.g., CAR or TCR) as disclosed herein. In some embodiments, the antigen recognition receptor can activate the immunoresponsive cell. The immunoresponsive cell of the present subject matter can be a cell of the lymphoid system. The lymphoid system, including B, T, and natural killer (NK) cells, provides for the production of antibodies, regulation of the cellular immune system, detection of foreign agents in the blood, detection of cells foreign to the host, and the like. Non-limiting examples of immunoresponsive cells of the lymphoid system include T cells, natural killer (NK) cells, embryonic stem cells, and pluripotent stem cells (e.g., from which lymphocytes can be differentiated). T cells can be lymphocytes that mature in the thymus and are primarily responsible for cell-mediated immunity. T cells are involved in the adaptive immune system. The T cells of the subject invention may be any type of T cell, including, but not limited to, helper T cells, cytotoxic T cells, memory T cells (central memory T cells, stem-cell-like memory T cells / stem-like memory T cells) and two types of effector memory T cells, such as TEM cells and TEMRA cells, regulatory T cells (also called inhibitory T cells), natural killer T cells, mucosal-associated invariant T cells, and gd T cells. Cytotoxic T cells (CTL or killer T cells) are a subset of T lymphocytes that can induce the death of infected somatic or tumor cells. The patient's own T cells can be genetically modified to target specific antigens by introducing an antigen recognition receptor (e.g., CAR or TCR). In some embodiments, the immunoresponsive cells are T cells. The T cells may be CD4+ T cells or CD8+ T cells. In some embodiments, the T cells are CD4+ T cells. In some embodiments, the T cells are CD8+ T cells. Natural killer (NK) cells may be lymphocytes that are part of cell-mediated immunity and function during the innate immune response. NK cells do not need to be pre-activated to exert a cytotoxic effect on target cells.The types of human lymphocytes that are the subject of the present invention include, but are not limited to, peripheral donor lymphocytes, as described, for example, in Sadelain, M., et al. 2003 Nat Rev Cancer 3:35-45 (disclosing peripheral donor lymphocytes genetically modified to express CARs), Morgan, RA, et al. 2006 Science 314: 126-129 (disclosing peripheral donor lymphocytes genetically modified to express full-length tumor antigen-recognizing T cell receptor complexes including a and b heterodimers), Panelli, MC, et al. 2000 J Immunol 164: 495-504, Panelli, MC, et al. 2000 J Immunol 164:4382-4392 (disclosing lymphocyte cultures derived from tumor-infiltrating lymphocytes (TILs) in tumor biopsies), and Dupont, J., et al. 2005 Cancer Res. 65:5417-5427; Papanicolaou, GA, et al. 2003 Blood 102:2498-2505 (disclosing the use of artificial antigen presenting cells (AAPCs) or pulsed dendritic cells to selectively expand antigen-specific peripheral blood leukocytes in vitro). In some embodiments, the immunoresponsive cells (e.g., T cells) may be autologous, non-autologous (e.g., allogeneic), or derived in vitro from engineered progenitor or stem cells.

[0193] 3.How to use The present subject matter further provides methods of using the disclosed antibodies and antibody derivatives. In some embodiments, these methods relate to therapeutic uses of the antibodies or antibody derivatives of the invention. In some embodiments, these methods relate to diagnostic uses of the antibodies or antibody derivatives of the invention.

[0194] 3.1 Treatment method The present invention provides methods and uses of the antibodies or antibody derivatives disclosed herein for treating diseases and disorders or enhancing immune responses. In some embodiments, the antibodies, antibody derivatives or pharmaceutical compositions comprising the antibodies, antibody derivatives disclosed herein can be administered to a subject (e.g., a mammal (e.g., a human)) to treat diseases and disorders or enhance immune responses. In some embodiments, these diseases and disorders are related to immune checkpoint inhibition and / or aberrant OX40 activity. In some embodiments, diseases and disorders treatable by the antibodies or antibody derivatives disclosed herein include, but are not limited to, neoplasms (e.g., cancer).

[0195] In some embodiments, the present invention provides an antibody or antibody derivative (or fragment thereof) described herein for use in the preparation of a medicament. In some embodiments, the present invention provides an antibody or antibody derivative (or fragment thereof) described herein for use in the preparation of a medicament for the treatment of cancer. In some embodiments, the present invention provides an antibody or antibody derivative (or fragment thereof) described herein for use in the treatment of cancer in a subject. In some embodiments, the present invention provides a pharmaceutical composition comprising an antibody or antibody derivative (or fragment thereof) according to the present invention for use in the treatment of cancer in a subject. In some embodiments, the cancer may be blood cancer (e.g., leukemia, lymphoma, and myeloma), ovarian cancer, breast cancer, bladder cancer, brain cancer, colon cancer, intestinal cancer, liver cancer, lung cancer, pancreatic cancer, prostate cancer, skin cancer, gastric tumor, glioblastoma, laryngeal cancer, melanoma, neuroblastoma, adenocarcinoma, glioma, soft tissue sarcoma, and miscellaneous cancers (including prostate cancer and small cell lung cancer). The applicable cancers further include any known cancer in the field of oncology, including astrocytoma, fibrosarcoma, myxosarcoma, liposarcoma, oligodendroglioma, ependymoma, medulloblastoma, primary neuroectodermal tumor (PNET), chondrosarcoma, osteogenic sarcoma, pancreatic ductal adenocarcinoma, small and large cell lung adenocarcinoma, chordoma, angiosarcoma, endothelial sarcoma, squamous cell carcinoma, bronchioloalveolar carcinoma, epithelial adenocarcinoma and their liver metastases, lymphangiosarcoma, lymphangioendothelial sarcoma, hepatocellular carcinoma, cholangiocarcinoma, synovium, mesothelioma, Ewing's tumor, rhabdomyosarcoma, colon carcinoma, basal cell carcinoma, sweat adenoma, papillary carcinoma, sebaceous gland carcinoma, papillary adenocarcinoma, cystadenocarcinoma, medullary carcinoma, bronchogenic carcinoma, These include, but are not limited to, renal cell carcinoma, cholangiocarcinoma, choriocarcinoma, seminoma, embryonal carcinoma, Wilms' tumor, testicular tumor, medulloblastoma, craniopharyngioma, ependymoma, pineal tumor, hemangioblastoma, acoustic neuroma, oligodendroglioma, meningioma, neuroblastoma, leukemia, multiple myeloma, Waldenstrom's macroglobulinemia, breast tumors (e.g., ductal and lobular adenocarcinoma), cervical squamous cell carcinoma and adenocarcinoma, uterine epithelial and ovarian epithelial carcinoma, prostate adenocarcinoma, transitional squamous cell carcinoma of the bladder, B and T lymphoma (nodular and dispersed), plasmacytoma, acute and chronic leukemia, malignant melanoma, soft tissue sarcoma, and leiomyosarcoma.

[0196] In some embodiments, the cancer may be melanoma, NSCLC, head and neck cancer, urothelial carcinoma, breast cancer (e.g., triple-negative breast cancer, TNBC), gastric cancer, cholangiocarcinoma, classical Hodgkin lymphoma (cHL), non-Hodgkin lymphoma primary mediastinal B-cell lymphoma (NHL PMBCL), mesothelioma, ovarian cancer, lung cancer (e.g., small cell lung cancer), esophageal cancer, nasopharyngeal carcinoma (NPC), biliary tract cancer, colorectal cancer, cervical cancer, or thyroid cancer.

[0197] In some embodiments, the subject to be treated is a mammal (e.g., human, non-primate, rat, mouse, cow, horse, pig, sheep, goat, dog, cat, etc.). In some embodiments, the subject is a human. In some embodiments, the subject is suspected of having cancer, or is at risk of having cancer, or has been diagnosed with cancer or any other disease with abnormal OX40 expression or activity.

[0198] Many cancer or any other disease diagnostic methods that show abnormal OX40 activity and clinical description of these diseases are known in the art. Such methods include, but are not limited to, immunohistochemistry, PCR, and fluorescent in situ hybridization (FISH). Other details regarding the diagnostic method of abnormal OX40 activity or expression are described, for example, in Gupta et al. (2009) Mod Pathol. 22(1): 128-133, Lopez-Rios et al. (2013) J Clin Pathol. 66(5): 381-385, Ellison et al. (2013) J Clin Pathol 66(2): 79-89, and Guha et al. (2013) PLoS ONE 8(6): e67782.

[0199] It may be administered by any suitable route, including, for example, intravenously, intramuscularly, or subcutaneously. In some embodiments, an antibody or antibody derivative (or fragment thereof) and / or composition according to the present invention may be administered in combination with a second, third, or fourth agent (including, for example, an anti-tumor agent, a growth inhibitory agent, a cytotoxic agent, or a chemotherapeutic agent) to treat a disease or disorder associated with aberrant OX40 activity. Such agents include, for example, docetaxel, gefitinib, FOLFIRI (irinotecan, 5-fluorouracil, and folinic acid), irinotecan, cisplatin, carboplatin, paclitaxel, bevacizumab (anti-VEGF antibody), FOLFOX-4, infused fluorouracil, folinic acid and oxaliplatin, alfaltinib, gemcitabine, capecitabine, pemetrexed, tecartinib, everolimus, CpG-ODN, rapamycin, lenalidomide, belofinil, endostatin, lapatinib, PX-866, Imprime PGG, and irinotinib. In some embodiments, the antibody or antibody derivative (or fragment thereof) is conjugated to another agent.

[0200] In some embodiments, an antibody or antibody derivative (or fragment thereof) and / or composition provided herein is administered in combination with one or more other therapies (e.g., radiation therapy, surgery, chemotherapy, and / or targeted therapy). In some embodiments, an antibody, antibody derivative (or fragment thereof) and / or composition provided herein is administered in combination with radiation therapy. In some embodiments, an antibody, antibody derivative (or fragment thereof) and / or composition provided herein is used to treat a neoplasm or cancer as disclosed herein in combination with radiation therapy.

[0201] Depending on the indication to be treated and factors related to administration known to those skilled in the art, the antibody or antibody derivative according to the present invention is administered in a dose effective to treat the indication while minimizing toxicity and side effects. In the treatment of cancer, a typical dose may be, for example, in the range of 0.001 to 1000 μg, however, doses lower or higher than the exemplary range are within the scope of the present invention. A daily dose may be about 0.1 μg / kg to about 100 mg / kg of total body weight, about 0.1 μg / kg to about 100 μg / kg of total body weight, or about 1 μg / kg to about 100 μg / kg of total body weight. As mentioned above, the therapeutic or prophylactic effect can be monitored by periodically evaluating the treated patient. For repeated administration over several days or more, treatment is repeated depending on the symptoms until the desired suppression of disease symptoms occurs. However, other dosing regimens may be useful and are within the scope of the present invention. The desired dose may be delivered by administration of a single bolus of the composition, multiple boluses of the composition, or continuous infusion of the composition.

[0202] A pharmaceutical composition comprising an antibody or antibody derivative disclosed herein may be administered once, twice, three or four times a day. The composition may be administered less frequently than daily, for example, six times weekly, five times weekly, four times weekly, three times weekly, two times weekly, once weekly, once every two weeks, once every three weeks, once monthly, once every two months, once every three months, or once every six months. The composition may be administered in a sustained release formulation, for example, in an implant, which gradually releases the composition for use over a period of time and allows the composition to be administered less frequently, for example, once monthly, once every 2-6 months, once yearly, or even once. A sustained release device (e.g., pellets, nanoparticles, microparticles, nanospheres, microspheres, etc.) may be administered by injection or surgical implantation at various locations.

[0203] Cancer treatment may be evaluated, for example, but not limited to, by tumor regression, tumor weight or size reduction, time to progression, survival time, progression-free survival, overall response rate, duration of response, quality of life, protein expression and / or activity. Methods for determining efficacy of treatment can be used, including, for example, measuring response by radiological imaging.

[0204] In some embodiments, the therapeutic effect is measured as percent tumor growth inhibition (% TGI) and is calculated using the equation 100-(T / C x 100), where T is the average relative tumor volume of treated tumors and C is the average relative tumor volume of untreated tumors. In some embodiments, the % TGI can be about 10%, about 20%, about 30%, about 40%, about 50%, about 60%, about 70%, about 80%, about 90%, about 91%, about 92%, about 93%), about 94%), about 95%, or greater than 95%.

[0205] 3.2 Diagnostic and imaging methods Labeled antibodies or antibody derivatives may be used diagnostically to detect, diagnose or monitor diseases and / or disorders associated with OX40 expression, aberrant expression and / or activity. For example, antibodies and antibody derivatives according to the present disclosure may be used in in situ, in vivo, ex vivo and in vitro diagnostic or imaging assays. Methods for detecting OX40 polypeptide expression include (a) measuring expression of the polypeptide in cells (e.g., tissues) or body fluids of an individual using one or more antibodies or antibody derivatives, and (b) comparing the gene expression level to a standard gene expression level, where an increase or decrease in the measured gene expression level compared to the standard expression level indicates aberrant expression.

[0206] Another embodiment according to the invention includes a method for diagnosing a disease or disorder involving expression or abnormal expression of OX40 in an animal (e.g., a mammal, e.g., a human). The method includes detecting an OX40 molecule in the mammal. In some embodiments, the diagnosis includes (a) administering an effective amount of a labeled antibody or antibody derivative to the mammal, (b) waiting a period of time after administration to allow the labeled antibody or antibody derivative to preferentially concentrate at sites expressing the OX40 molecule in the subject (and remove unbound, labeled molecules to background levels), (c) determining a background level, and (d) detecting the labeled molecule in the subject, such that detection of a labeled molecule higher than the background level indicates that the subject suffers from a particular disease or disorder involving expression or abnormal expression of OX40. The background level may be determined in different ways, and these methods include comparing the amount of detected labeled molecule to a standard value previously determined for one specific system.

[0207] The antibodies and antibody derivatives herein may be used to measure protein levels in biological samples using classical immunohistology methods well known to those of skill in the art (see, e.g., Jalkanen, et al., J. Cell. Biol. 101:976-985 (1985); Jalkanen, et al., J. Cell. Biol. 105:3087-3096 (1987)). Other antibody-based methods useful for detecting protein gene expression include immunoassays, such as enzyme-linked immunosorbent assays (ELISAs) and radioimmunoassays (RIAs). Suitable antibody assay labels are known in the art and include enzyme labels (e.g., glucose oxidase), radioisotopes, such as iodine ( 131 I, 125 I, 123 I, 121 I), Carbon ( 14 C), sulfur ( 35 S), tritium ( 3 H), Indium ( 115m In,113m In, 112 In, 111 In), and technetium ( 99 Tc, 99m Tc), thallium ( 201 Ti), Gallium ( 68 Ga, 67 Ga), palladium ( 103 Pd), Molybdenum ( 99 Mo), Xenon ( 133 Xe), fluorine ( 18 F), 153 Sm, 177 Lu, 159 Gd, 149 Pm, 140 La, 175 Yb, 166 Ho, 90 Y, 47 Sc, 186 Re, 188 Re, 142 Pr, 105 Rh, 97 These include Ru, luminol, and fluorescent labels (eg, fluorescein, rhodamine, and biotin).

[0208] Techniques known in the art can be applied to labeling antibodies (or fragments thereof) according to the present disclosure, including, but not limited to, the use of bifunctional conjugates (see, e.g., U.S. Patent Nos. 5,756,065, 5,714,631, 5,696,239, 5,652,361, 5,505,931, 5,489,425, 5,435,990, 5,428,139, 5,342,604, 5,274,119, 4,994,560, and 5,808,003).

[0209] Alternatively, or additionally, the level of nucleic acid or mRNA encoding an OX40 polypeptide in the cells may be measured, for example, by fluorescent in situ hybridization (FISH, see WO 1998 / 45479, disclosed October 1998), DNA blotting, RNA blotting or polymerase chain reaction (PCR) techniques, such as real-time quantitative PCR (RT-PCR), using a nucleic acid-based probe corresponding to a nucleic acid encoding OX40 or its complementary sequence. Overexpression of OX40 can also be studied by measuring shed antigen in biological fluids (e.g., serum), for example, using antibody-based assays (see, for example, U.S. Pat. No. 4,933,294, published June 12, 1990; WO 91 / 05264, published April 18, 1991; U.S. Pat. No. 5,401,638, published March 28, 1995; and Sias et al., J. Immunol. Methods 132:73-80 (1990)). In addition to the above assays, those skilled in the art can obtain various in vivo and ex vivo assays. For example, cells in a mammalian body can be exposed to an antibody, which is optionally labeled with a detectable label (e.g., a radioisotope), and the binding of the antibody to the cells can be evaluated, for example, by radioactive external scanning or by analyzing a sample (e.g., a biopsy or other biological sample) taken from a mammal previously exposed to the antibody.

[0210] 4. Drug Formulations The present subject matter further provides a pharmaceutical formulation comprising an antibody or antibody derivative disclosed herein and a pharma- ceutically acceptable carrier agent. In some embodiments, a pharmaceutical composition may comprise a combination of multiple (e.g., two or more) antibodies and / or antibody derivatives of the present subject matter.

[0211] In some embodiments, the disclosed drug formulations may be prepared by combining an antibody or antibody derivative having a desired degree of purity with one or more optional pharma- ceutically acceptable carrier agents (Remington's Pharmaceutical Sciences 16th edition, Osol, A. Ed (1980)) and may take the form of a lyophilized formulation or an aqueous solution. For example, frozen antibody formulations are described in, but are not limited to, U.S. Pat. No. 6,267,958. In some embodiments, aqueous antibody formulations may include those described in U.S. Pat. No. 6,171,586 and WO 2006 / 044908, the latter formulations including histidine-acetate buffers. In some embodiments, the antibody or antibody derivative may have a purity of greater than about 80%, greater than about 90%, greater than about 91%, greater than about 92%, greater than about 93%, greater than about 94%, greater than about 95%, greater than about 96%, greater than about 97%, greater than about 98%, greater than about 99%, greater than about 99.1%, greater than about 99.2%, greater than about 99.3%, greater than about 99.4%, greater than about 99.5%, greater than about 99.6%, greater than about 99.7%, greater than about 99.8%, or greater than about 99.9%.

[0212] Pharmaceutically acceptable carrier agents are typically nontoxic to recipients at the dosages and concentrations employed, and include buffers (e.g., phosphates, citrates, and other organic acids), antioxidants including ascorbic acid and methionine acid, preservatives (e.g., benzyldimethyloctadecylammonium chloride, hexamethonium chloride, benzalkonium chloride, phenethylammonium chloride, phenol, butanol or benzyl alcohol, alkylparabens (e.g., methyl or propylparaben), catechol, resorcinol, cyclohexanol, 3-pentanol, and m-cresol), low molecular weight (less than about 10 residues), and / or other suitable carriers. ) polypeptides, proteins such as serum albumin, gelatin or immunoglobulins, hydrophilic polymers such as polyvinylpyrrolidone, amino acids such as glycine, glutamine, asparagine, histidine, arginine, or lysine, monosaccharides, disaccharides, and other carbohydrates including glucose, mannose, or dextrins, chelating agents such as EDTA, sugars such as sucrose, mannitol, trehalose, or sorbitol, chloride counterions such as sodium, metal complexes (e.g., Zn-protein complexes), and / or non-ionic surfactants such as polyethylene glycol (PEG). Exemplary pharmacologic carrier agents herein further include mesenchymal drug dispersion agents, such as soluble neutral active hyaluronidase glycoproteins (sHASEGPs), such as human soluble PH-20 hyaluronidase glycoproteins, such as rHuPH20 (HYLENEX®, Baxter International, Inc.). US Patent Nos. 2005 / 0260186 and 2006 / 0104968 describe several exemplary sHASEGPs, including rHuPH20, and methods of use. In some embodiments, the sHASEGP is combined with one or more additional glycosaminoglycanases (e.g., chondroitinases).

[0213] The carrier agent may be suitable for intravenous, intramuscular, subcutaneous, parenteral, spinal or epidermal administration (e.g., by injection or infusion). Depending on the route of administration, the active compound (e.g., an anti-OX40 antibody or multispecific antibody disclosed herein) may be coated with a material to protect the compound from the effects of acids and other natural conditions that may inactivate the compound.

[0214] The pharmaceutical composition of the present invention may be used in combination therapy, i.e., may be administered in combination with other drugs. In some embodiments, the pharmaceutical composition disclosed herein may further comprise more than one active ingredient, which is essential for the particular indication being treated, e.g., has complementary activities and does not adversely affect each other. In some embodiments, the drug formulation may comprise a second active ingredient for treating the same disease being treated by the first therapeutic agent. Such active ingredients are present in appropriate combination in an amount effective for the desired purpose. For example, the formulation of the present invention may further comprise more than one active ingredient, which is essential for the particular indication being treated, preferably has complementary activities and does not adversely affect each other. For example, it may be desirable to further provide a second therapeutic agent for treating the same disease. Such active ingredients are present in appropriate combination in an amount effective for the desired purpose.

[0215] The compositions of the present invention may be administered in various ways known in the art. The route and / or mode of administration depends on the desired results. These active compounds may be prepared with a carrier that protects the compound from rapid release, for example, controlled release formulations, including implants, transdermal patches, and microencapsulated delivery systems. Biodegradable and biocompatible polymers may be used, for example, ethylene vinyl acetate, polyanhydrides, polyglycolic acid, collagen, polyorthoesters, and polylactic acid. Many methods for preparing such formulations are described, for example, in Sustained and Controlled Release Drug Delivery Systems, JR Robinson, ed, Marcel Dekker, Inc., New York, 1978. In some embodiments, the pharmaceutical compositions are produced under U.S. Food and Drug Administration Good Manufacturing Practice (GMP) conditions.

[0216] A sustained release formulation may be prepared that includes the antibody or antibody derivative disclosed herein. A suitable example of a sustained release formulation includes a semipermeable matrix containing solid hydrophobic polymers of the antibody or antibody derivative, the matrix being in the form of a shaped article (e.g., a film or a microcapsule). In some embodiments, the active ingredient can be embedded in a microcapsule prepared, for example, by coacervation techniques or interfacial polymerization, for example, hydroxymethylcellulose or gelatin-microcapsules and poly(methyl methacrylate) microcapsules in colloidal drug delivery systems (e.g., liposomes, albumin microspheres, microemulsions, nanoparticles and nanocapsules) or crude emulsions, respectively. Such techniques are disclosed in Remington's Pharmaceutical Sciences 16th edition, Osol, A. Ed. (1980).

[0217] To administer the antibody or antibody derivative of the present invention by some administration routes, it may be necessary to coat the compound with or administer the compound with a material that prevents its inactivation. For example, the compound may be administered to a subject in a suitable carrier agent (e.g., liposome) or diluent. Pharmaceutically acceptable diluents include saline and aqueous buffer solutions. Liposomes include water-in-oil-in-water CGF emulsions and conventional liposomes (Strejan et al. (1984) J Neuroimmunol. 7:27).

[0218] Pharmaceutically acceptable carriers include sterile aqueous solutions or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersions. The substances used for such media and agents for pharmaceutical activity are well known in the art.

[0219] Except insofar as any conventional media or agent is incompatible with the active compound, its use in the pharmaceutical compositions of the present invention is contemplated. Supplementary active compounds may also be doped into the compositions.

[0220] Therapeutic compositions should usually be sterile, essentially isotonic, and stable under production and storage conditions. The compositions may be formulated as solutions, microemulsions, liposomes, or other ordered structures suitable for high drug concentration. The carrier may be a solvent or dispersion medium containing, for example, water, ethanol, polyol (for example, glycerol, propylene glycol, and liquid polyethylene glycol, and the like), and suitable mixtures thereof. Proper fluidity may be maintained by the use of (for example) a coating (for example, lecithin), by the maintenance of a desired particle size in the case of dispersions, and by the use of surfactants. In many cases, it is preferred to include an isotonic agent in the composition, for example, sugar, polyol (for example, mannitol, sorbitol), or sodium chloride. Prolonged absorption of these injectable compositions can be achieved by including in the composition an agent that delays absorption, such as monostearate and gelatin.

[0221] Sterile injectable solutions may be prepared by doping a desired amount of one or more of the antibodies or antibody derivatives disclosed herein with a suitable solvent and one or more of the above-listed ingredients, if necessary, followed by sterilization microfiltration (e.g., filtration through a sterile filtration membrane). Usually, dispersions are prepared by doping the active compound into a sterile medium, which contains a basic dispersion medium and other desired ingredients from those listed above. In the case of sterile powders for the preparation of sterile injectable solutions, the preferred preparation methods are vacuum drying and freeze-drying (lyophilization), which produce a powder of the active ingredient and any other desired ingredients from the previously sterile-filtered solution.

[0222] Therapeutic compositions can also be administered using medical devices known in the art. For example, therapeutic compositions of the present invention can be administered with needleless hypodermic injection devices, such as those disclosed in U.S. Patent Nos. 5,399,163, 5,383,851, 5,312,335, 5,064,413, 4,941,880, 4,790,824, or 4,596,556. Examples of implants and modules that may be used in the present invention include U.S. Patent No. 4,487,603, which discloses an implantable micro infusion pump for dispensing drugs at a controlled rate, U.S. Patent No. 4,486,194, which discloses a therapeutic device for administering drugs through the skin, U.S. Patent No. 4,447,233, which discloses a drug infusion pump for delivering drugs at precise infusion rates, U.S. Patent No. 4,447,224, which discloses a variable flow rate implantable infusion device for continuous drug delivery, U.S. Patent No. 4,439,196, which discloses an osmotic drug delivery system having multiple compartments, and U.S. Patent No. 4,475,196, which discloses an osmotic drug delivery system. Many such implants, delivery systems, and modules are known.

[0223] For therapeutic compositions, the formulations of the present invention include those suitable for oral, nasal, topical (including buccal and sublingual), rectal, vaginal and / or parenteral administration. These formulations may conveniently be present in unit dosage form and may be prepared by any method known in the pharmaceutical art. The amount of antibody or antibody derivative that can be combined with a carrier material to produce a single dosage form varies depending on the subject being treated and the particular mode of administration. The amount of antibody or antibody derivative that can be combined with a carrier material to produce a single dosage form is usually that amount of the composition that produces a therapeutic effect. Usually, the amount is, in percentage, about 0.01% to about 99%, about 0.1% to about 70%, or about 1% to about 30% of active ingredient.

[0224] Dosage forms for topical or transdermal administration of the compositions of the present invention include powders, sprays, ointments, pastes, creams, lotions, gels, solutions, patches and inhalants. The active compound may be mixed under sterile conditions with a pharma- ceutically acceptable carrier and any preservatives, buffers or propellants which may be required.

[0225] The phrases "parenteral administration" and "administration by parenteral means" refer to modes of administration other than enteral and topical administration, usually by injection, and include, but are not limited to, intravenous, intramuscular, intraarterial, intrathecal, intracapsular, intraorbital, intracardiac, intradermal, intraperitoneal, transtracheal, subcutaneous, subcuticular, intraarticular, subcapsular, subarachnoid, intraspinal, epidural, and intrasternal injection and infusion.

[0226] These pharmaceutical compositions may further contain auxiliary agents, such as preservatives, wetting agents, emulsifying agents, and dispersing agents. Prevention of the presence of microorganisms can be ensured by the above sterilization procedures and by the inclusion of various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol sorbic acid, and the like. It may also be desirable to include isotonic agents, for example, sugars, sodium chloride, and the like, in these compositions. In addition, prolonged absorption of the injectable drug form can be brought about by the inclusion of agents which delay absorption, for example, aluminum monostearate and gelatin.

[0227] In some embodiments, when the antibodies or antibody derivatives of the present invention are administered to humans and animals as drugs, they can be administered alone or in combination with a pharma- ceutical acceptable carrier agent as a pharmaceutical composition comprising, for example, about 0.01% to about 99.5% (or about 0.1% to about 90%) of the antibody or antibody derivative.

[0228] 5.Product The presently disclosed subject matter further provides articles of manufacture containing materials for use in the treatment, prevention and / or diagnosis of the above-mentioned disorders.

[0229] In some embodiments, the product includes a container and a label or packaging insert on or associated with the container. Non-limiting examples of suitable containers include bottles, vials, syringes, IV solution bags, and the like. The container may be formed of a variety of materials (e.g., glass or plastic). The container holds a composition that is effective for treating, preventing, and / or diagnosing a disease (by itself or in combination with another composition) and may have a sterile access port (e.g., the container may be an intravenous solution bag or a vial with a stopper that can be pierced by a hypodermic needle).

[0230] In some embodiments, at least one active agent in the composition is an antibody or antibody derivative of the invention. The label or packaging insert can indicate that the composition is used for treating a selected condition.

[0231] In some embodiments, the article of manufacture may include (a) a first container containing a composition comprising an antibody or antibody derivative of the invention, and (b) a second container containing a composition comprising another cytotoxic or therapeutic agent. In some embodiments, the article of manufacture may further include a packaging insert indicating that the composition may be used to treat a particular condition.

[0232] Alternatively, or additionally, the article of manufacture may further comprise another container, e.g., a second or third container, which includes, but is not limited to, a pharma- ceutically acceptable buffer, such as, but not limited to, bacteriostatic water for injection (BWFI), phosphate-buffered saline, Ringer's solution, and dextrose solution. The article of manufacture may include other materials desirable from a commercial and user standpoint, including other buffers, diluents, filters, needles, and syringes.

[0233] [Table 3] [Table 4] [Table 5] [Table 6] [Table 7] [Table 8] [Table 9] [Table 10] [Table 11] [Table 12] [Table 13] [Table 14] [Table 15] [Table 16] [Table 17]

[0234] The following examples are merely illustrative of the subject matter of the present invention and should not be construed as limiting in any manner. EXAMPLES

[0235] Example 1 Immunization, generation and recognition of anti-OX40 VHH antibodies Recombinant human OX40 extracellular domain (ECD) proteins conjugated to His-tag (OX40 ECD-His) or human IgG1-Fc (OX40-ECD-Fc) were prepared in-house and used to immunize llamas according to standard procedures. Serum antibody titers were measured by ELISA, and after four immunizations (first with OX40 ECD-His and third with OX40 ECD-Fc), antibody titers reached ≥1:100,000. Whole blood was then collected and used for PBMC isolation. Total RNA was extracted from purified PBMCs, reverse transcribed to generate cDNA, and further amplified by PCR according to standard procedures. VHH antibody gene fragments were amplified by PCR, gel purified, subcloned into the phagemid vector pADL-23c (Antibody Design Labs #PD0111), and transformed into TG1 electroporation recipient cells (Lucigen). Transformed TG1 cells were cultured in 2xYT medium, helper phages were added, and co-cultured overnight to produce phages displaying the target VHH. Phages in the culture supernatant were harvested by centrifugation and subjected to three rounds of panning using streptavidin-coupled Dynabeads M280 (ThermoFisher#11205D), coated with biotinylated human OX40 ECD-Fc in the first round, and with biotinylated human OX40 ECD-His in the second and third rounds. Phages displaying human OX40 binders were eluted and used to infect SS320 cells. Colonies were picked and cultured in IPTG-containing 2xYT medium to secrete VHH antibodies. Supernatants containing VHH antibodies were screened by ELISA using human OX40 ECD-Fc or human OX40 ECD-His pre-coated plates. The top human OX40 binding proteins were selected and sequenced, including 5E10, whose CDR and VHH sequences were as shown in the sequence listing (SEQ ID NO: 1-5).

[0236] Llama VHH clone 5E10 was fused to human IgG1 Fc to form a chimeric bivalent antibody (c5E10), as shown in FIG. 1A and in the sequence listing. The protein was expressed in ExpiCHO cells and purified by Protein A affinity chromatography. The binding affinity of bivalent c5E10 to human and mouse OX40 was measured by ELISA. Briefly, human or mouse OX40 ECD fused to 1 μg / ml Fc was coated on 96-well high-binding plates and incubated overnight at 4° C. After blocking with 3% BSA in PBS, 5-fold serially diluted biotin-labeled c5E10 was added and incubated for 1 h at RT. After washing 5 times with PBS+0.05% Tween® 20, bound c5E10 was detected by HRP-conjugated avidin followed by TMB substrate. As shown in FIG. 1B, c5E10 bound to human OX40 in a dose-dependent manner, but not to mouse OX40.

[0237] OX40 ECD contains four cysteine-rich domains (CRDs) that allow the binding of its ligand, leading to receptor aggregation and activation of downstream signals. Because c5E10 does not bind to mouse OX40, human-mouse OX40 chimeras were prepared in which each human OX40 CRD was replaced with its mouse OX40 counterpart, which was used to recognize the c5E10-bound OX40 CRD domain, as shown in FIG. 1C. Human OX40 ECD or human-mouse chimeric ECD was fused to human IgG1 Fc, and the fusion protein was purified by Protein A affinity chromatography. 1 μg / ml Fc-fused human OX40 ECD or human-mouse chimeric OX40 ECD was coated on a 96-well high-binding plate, incubated overnight at 4° C., and the binding of biotin-labeled OX40 antibodies to these proteins was measured by ELISA as described above. Replacement of human CRD2 almost completely eliminated the binding of c5E10 to human OX40 (Figure 1D), indicating that c5E10 binds to human OX40 CRD2. Anti-OX40 antibodies PF-8600 and MEDI0562 were used as controls. PF-8600 was synthesized in-house based on the sequence disclosed in U.S. Patent No. 7,960,515. MEDI0562 was synthesized in-house based on the sequence disclosed in U.S. Publication No. 2016 / 0137740A1. As shown in Figure 1D, the PF-8600 analog did not bind to the mD1 chimera, indicating that the PF-8600 analog binds to human OX40 CRD1, and the binding of the MEDI0562 analog to mD3 was sharply reduced, indicating that the MEDI0562 analog binds to human OX40 CRD3.

[0238] To compare the binding epitopes of c5E10, the PF-8600 analogs and the MEDI0562 analogs, Octet competitive binding assays were performed using anti-human IgG Fc (AHC) biosensors loaded first with one antibody and then with the other antibody shown in Table 3. As shown in Table 3, c5E10 did not compete with the PF-8600 analogs or the MEDI0562 analogs, consistent with the fact that they bound to different OX40 CRDs.

[0239] [Table 18]

[0240] Example 2. Humanization and affinity maturation of anti-OX40 VHH antibodies IgBlast analysis was performed using the llama 5E10 sequence to search for human germline genes in databases. Humanization was achieved by grafting the CDRs of llama 5E10 into the best-matched human germline IGHV3-23 and reverting the mutations V37F and W47F within framework 2, because these two Phe(F) are llama-tagged residues. For affinity maturation, NNK-based primers were designed to encode highly variable CDR1, CDR2, and CDR3. Using PCR assembly, a library containing site-saturation mutagenesis was prepared and cloned into the phagemid vector pADL-23c. Library DNA was transformed into TG1 cells and clone sequences were checked to ensure random quality. After two rounds of panning by incubating unheated or heat-treated phages with human OX40 ECD-His-coated beads, eluted phages were used to infect SS330 cells, and colonies were picked and grown in 2xYT medium containing IPTG. The binding of VHH antibodies to human OX40 in the supernatant was measured by ELISA using human OX40 ECD-Fc or human OX40 ECD-His pre-coated plates. The first 15 clones and their CDR and VHH sequences are shown in the sequence listing (SEQ ID NO:6-80).

[0241] Bivalent antibodies of these 15 clones were prepared by fusing VHH with human IgG1 Fc, expressed in ExpiCHO cells, and purified by Protein A affinity column. The binding affinity of VHH-Fc bivalent antibodies to human or cynomolgus OX40 was evaluated by flow cytometry using Jurkat cells transfected with human OX40 (human OX40 / Jurkat) (Figure 2A), CHO cells transfected with human OX40 (human OX40 / CHO) (Figure 2B), and CHO cells transfected with cynomolgus OX40 (cynomolgus OX40 / CHO) (Figure 2C). The antibodies with the indicated concentrations and cells were incubated on ice for 30 min in FACS buffer (PBS containing 3% FBS), and free antibodies were washed off with FACS buffer. The cells were further incubated with anti-human IgG Fc antibody (1:500) coupled to Alexa fluor488 (Alexa Fluor488 AffiniPure goat anti-human IgG, Fcg fragment specific, Jackson labs) on ice for an additional 30 min. The cells were washed to remove free antibody and subjected to FACS analysis using CytoFlex (Beckman Coulter). Binding affinities were calculated with GraphPad Prism3 parametric logistic equations. As shown in Figure 2A-2C, these clones bound to both human OX40-expressing cells and cynomolgus OX40-expressing cells.

[0242] Example 3. In vitro characterization of anti-OX40 antibodies Anti-OX40 tetravalent antibodies were constructed in which human IgG1 heavy and light chain variable regions were replaced with clone 1B3 or clone 2B7 VHH sequences, and GS linkers were inserted between VHH and CH1 or CL of IgG1. The structure of the tetravalent antibodies was shown in Figure 3A. The tetravalent antibodies were produced by instantaneously transfected ExpiCHO cells and purified by Protein A affinity column. The binding kinetics and affinity of 1B3 and 2B7 bivalent and tetravalent antibodies to human OX40 were measured by Octet binding assay using human OX40 ECD-Fc immobilized on a biosensor. The equilibrium dissociation constants (KD) of 1B3 and 2B7 bivalent and tetravalent antibodies were calculated (Table 4). Both bivalent and tetravalent antibodies showed high binding affinity to human OX40.

[0243] [Table 19]

[0244] Using the above procedure, the binding affinity of anti-OX40 antibodies was further evaluated by flow cytometry using human OX40 / Jurkat cells and human OX40 / CHO cells. As shown in Figures 4A and 4B, both bivalent and tetravalent antibodies showed significantly high binding affinity to Jurkat and CHO cells expressing human OX40. As shown in Figure 4C, the tetravalent antibody did not bind to untransfected CHO cells, indicating that the antibody specifically interacts with human OX40 expressed on transfected cells. Cynomolgus OX40 / CHO cells were used to evaluate the cross-reactivity of antibodies to cynomolgus OX40 by FACS analysis. Compared to human OX40, both 1B3 and 2B7 antibodies bound to cynomolgus OX40 with similar affinity (Figure 4D).

[0245] The costimulatory activity of anti-OX40 antibodies (1B3-tetra and 2B7-tetra) was evaluated by stimulating human peripheral blood lymphocytes (PBMCs) with Staphylococcal enterotoxin B (SEB). In assays without FcγRIIB cross-linking, human PBMCs were stimulated with 100ng / ml SEB (Toxin Technology, cat. BT202red) and incubated with 1:5 gradient dilutions of 1B3-tetra or 2B7-tetra at 37°C in a 5% CO2 incubator for 2 days. In assays with cross-linking through FcγRIIB binding, 10,000 FcγRIIB / HEK293 cells were seeded overnight in a 96-well plate. The next day, human PBMCs were added and stimulated with 100ng / ml SEB in the presence of 1B3-tetra or 2B7-tetra for 2 days. IL-2 was quantified in the culture supernatant using a human IL-2 LANCE Ultra TR-FRET detection kit (PerkinElmer, cat. TRF1221). As shown in Figure 5A, when PBMC and FcγRIIB / HEK293 were co-cultured, 1B3-tetra and 2B7-tetra increased the expression of IL-2 in a dose-dependent manner. Without cross-linking with FcγRIIB, anti-OX40 antibodies could not enhance the release of IL-2 in SEB-activated PBMC (Figure 5B). The results suggest the clinical safety of anti-OX40 antibodies, because anti-OX40 agonist antibodies can activate OX40 signals without the need for cross-linking mediated by the inhibitory receptor FcγRIIB, and can cause peripheral toxicity by activating OX40 signals in peripheral tissues with low FcγRIIB expression.

[0246] The agonistic effect of 2B7-tetra on T cell activation and proliferation was further studied and compared with two reference anti-OX40 antibodies. Reference 1 was synthesized in-house based on IBI-101, the sequence of which was disclosed in US20200140562A1. Reference 2 was synthesized in-house based on BG445-3, the sequence of which was disclosed in WO 2019223733A1. Purified T cells (0.1x10 6 T cells (1000 / well) were stimulated with the indicated OX40 antibodies and anti-CD3 beads (ThermoFisher Scientific, cat. 11151D) (beads:T cells ratio = 1:1) for 3 days. Human IL-2 and IFNγ (PerkinElmer, cat. TRF1217) LANCE Ultra TR-FRET detection kit was employed to measure IL-2 and IFNγ in the culture supernatant. When T cells and FcγRIIB / HEK293 were co-cultured, the ability of 2B7-tetra and reference antibodies to costimulate T cells was demonstrated by the concentration-dependent increase in IL-2 and IFNγ secretion (Figures 6A and 6C). In the absence of FcγRIIB / HEK293, these anti-OX40 antibodies failed to enhance IL-2 or IFN-γ release from anti-CD3-stimulated T cells (Figures 6B and 6D).

[0247] To evaluate the effect of anti-OX40 antibody on T cell proliferation, purified T cells were co-cultured with mitomycin C-treated FcγRIIB / HEK293 and stimulated with anti-CD3 beads and anti-OX40 antibody for 5 days. Then, 20 μl 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazole (MTS, Promega, cat. G3580) was added to each well, and OD490 was measured after color development. As shown in Figures 7A and 7B, 2B7-tetra and the reference antibody promoted T cell proliferation when cross-linked by FcγRIIB expressed on HEK293 (Figure 7A), and these antibodies were inactivating when FcγRIIB / HEK293 was not co-cultured with T cells (Figure 7B).

[0248] In addition to the MTS assay, carboxyfluorescein isosuccinimide ester (CFSE)-labeled T cells were co-cultured with mitomycin C-treated FcγRIIB / HEK293 and stimulated with anti-CD3 beads and anti-OX40 antibody for 5 days. Then, cells were stained with Sytox Red to distinguish live and dead cells before FACS analysis. Figure 7C shows the population of live cells in the Sytox Red negative gate, where CFSE-negative cells were FcγRIIB / HEK293 cells, cells containing high CFSE were non-expanded resting T cells, and cells containing diluted CFSE levels were expanded T cells. The results showed that OX40 antibody promoted T cell proliferation, with an increase in CFSE-diluted expanded T cells and a decrease in resting T cells. The percentage of expanded T cells was further calculated by the following formula: expanded T cells / (expanded T cells+non-expanded T cells)*100, and shown in Figure 7D. The results showed that 2B7-tetra and the reference antibody induced T cell proliferation in a dose-dependent manner, similar to that observed in the MTS assay.

[0249] Example 4. In vivo characterization of anti-OX40 antibodies in the MC38 colon tumor model The antitumor effect of anti-OX40 antibodies was evaluated in the MC38 tumor model. Mouse MC38 colon tumor cells were subcutaneously implanted into human OX40 knock-in C57BL / 6 mice (Biocytogen, Boston, USA). Tumor size was approximately 60 mm. 3 When the tumor volume reached 100 mg / kg, the mice were randomly divided into groups of 8 mice each and administered 2B7-tetra intraperitoneally twice a week for 3 weeks. The tumor volume (TV) was calculated using the following formula: V=0.5*(a*b 2 ), where a and b are the long and short diameters of the tumor, respectively. The tumor growth inhibition rate (TGI) was calculated using the following formula: TGI = [1-(T t -T0) / (C t -C0)]×100%. Here, T0 and T t are the mean TVs at time 0 and t in the antibody treatment group, and C0 and C t is the average TV at time 0 and t in the vehicle group. 2B7 showed a dose-dependent inhibitory effect on MC38 tumor growth, and 15 days after treatment, tumor growth was inhibited by 19.5% and 61.0% by 1 mg / kg and 10 mg / kg of 2B7, respectively (Figure 8A). The difference in mouse body weight between each group was very small (Figure 8B), and no toxicity phenomenon was observed during treatment.

[0250] To compare the antitumor activity of 2B7 with the above-mentioned Reference 1 and Reference 2, mice were further intraperitoneally injected with 3 mg / kg of each antibody twice a week for 3 weeks. As shown in Figure 8C, 2B7 showed more effective antitumor activity compared with the two reference antibodies throughout the study. 17 days after treatment, the TGI of 2B7, Reference 1 and Reference 2 were 48.8%, 41.7% and 35.3%, respectively. Figure 8D shows the change in individual tumor volume over time in each group.

[0251] Example 5. In vivo characterization of anti-OX40 antibodies in the CT26 colon cancer model A CT26 colon cancer model was established in human OX40 knock-in BALB / c mice and used to analyze the antitumor activity of anti-OX40 antibody (Crownbio, Taicang, China). CT26 tumor size was approximately 65 mm 3When the tumor size reached 18 days after treatment, the mice were randomly divided into groups, with 8 mice per group, and treated with 2B7-tetra and reference 2 antibodies by intraperitoneal injection twice a week. As shown in Figure 9A, 2B7 showed a dose-dependent antitumor effect at 3 mg / kg and 10 mg / kg compared to the vehicle group. At the same dose of 3 mg / kg, 2B7 showed a stronger antitumor effect than reference 2. Specifically, on the 18th day after treatment, the average tumor size in the vehicle control group was 2393.1 mm 3 The mean tumor volumes in the 3 mg / kg and 10 mg / kg 2B7-tetra groups were 1321.7 and 825.5 mm, respectively. 3 In mice receiving 3 mg / kg reference 2, the mean tumor volume and TGI on day 18 was 1955.2 mm 3 , 18.8%. The change in individual tumor volume over time in each group is shown in Figure 9B. The average mouse body weights in the 2B7 and Reference 2 treatment groups were not significantly different from the vehicle group (Figure 9C), indicating that 2B7 and Reference 2 were well tolerated in this study.

[0252] Example 6. In vivo characterization of anti-OX40 antibodies in the Pan02 pancreatic tumor model Compared with MC38 and CT26 tumor models, the Pan02 pancreatic tumor model had significantly fewer effector T cells, inhibited infiltrating T cells, and was generally less sensitive to checkpoint inhibitors. The antitumor activity of 2B7-tetra alone or in combination with the murine PD1 antibody RMP1-14 was studied in the Pan02 tumor model. The Pan02 cell line was subcutaneously implanted into human OX40 knock-in C57BL / 6 mice (Crownbio, Taicang, China). The tumor size was 92.6 mm 3When the mice reached 1 mg / kg, they were randomly divided into groups, 10 mice per group, and intraperitoneally administered 1 or 5 mg / kg 2B7, or 5 mg / kg Reference 2 antibody once every 3 days. As shown in Figure 10A, 5 mg / kg 2B7 was statistically more effective than 5 mg / kg Reference 2 (p=0.014). On day 38, the TGI of 1 mg / kg 2B7, 5 mg / kg 2B7, and 5 mg / kg Reference 2 was 64.7%, 78.1%, and 55.5%, respectively.

[0253] Sequential administration of anti-OX40 and anti-PD1 antibodies was reportedly more effective than single antibody treatment and simultaneous administration of anti-OX40 and anti-PD1 antibodies in several mouse models. To determine whether the sequence and timing of anti-OX40 and anti-PD1 combination treatments were critical in the Pan02 tumor model, the study further included: (1) mice were co-administered with 5 mg / kg 2B7 and 2 mg / kg RMP1-14 once every 3 days for a total of 12 doses; (2) mice were administered 5 mg / kg 2B7 on days 0, 3, 6, 18, 21, and 24, and 2 mg / kg RMP1-14 on days 9, 12, 15, 27, 30, and 33; and (3) mice were administered 2 mg / kg RMP1-14 on days 0, 3, 6, 18, 21, and 24, and 5 mg / kg 2B7 on days 9, 12, 15, 27, 30, and 33. As shown in Figure 10B, the anti-PD1 antibody RMP1-14 alone was ineffective in the Pan02 tumor model, with 2 mg / kg RMP1-14 having a TGI of 23.2% on day 38. Mice administered 2B7 followed by delayed administration of RMP1-14 showed better antitumor activity than the simultaneous treatments, and RMP1-14 administered first followed by delayed administration of 2B7 had the least activity of the combination regimens (Figure 10B). On day 38, the TGIs of the simultaneous treatment, combination treatment with RMP1-14 and delayed 2B7, and combination treatment with 2B7 and delayed RMP1-14 were 68.0%, 30.4%, and 69.8%, respectively. Unexpectedly, 5 mg / kg 2B7 alone showed a higher TGI (78.1%) than all combination treatments. There was no significant difference in mouse body weight among these groups (Figure 10C). The change in individual tumor volume over time in each treatment group is shown in Figure 10D.

[0254] In addition to the various embodiments shown and claimed, the disclosed subject matter is further directed to other embodiments having other combinations of the features disclosed and claimed herein. Thus, the specific features presented herein may be combined with each other in other ways within the scope of the disclosed subject matter such that the disclosed subject matter includes any suitable combination of the features disclosed herein. The above description of specific embodiments of the disclosed subject matter has been provided for purposes of illustration and description. The above description is not intended to be exhaustive or to limit the disclosed subject matter to those embodiments disclosed.

[0255] It will be apparent to those skilled in the art that various modifications and variations can be made in the structure and methodology of the disclosed subject matter without departing from the spirit or scope of the disclosed subject matter. Therefore, it is intended that the disclosed subject matter cover such modifications and variations that come within the scope of the appended claims and their equivalents.

[0256] Various publications, patents, and patent applications are cited herein, the contents of which are incorporated by reference in their entireties.

Claims

1. An antibody that binds to OX40, a single domain antibody and a heavy chain variable region, the heavy chain variable region comprising: a) a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO: 1, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO: 2, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO: 3; b) a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO: 6, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO: 7, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO: 8; c) a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO: 11, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO: 12, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO: 13; d) a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO: 16, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO: 17, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO: 18; e) a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO: 21, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO: 22, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO: 23; f) a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO: 26, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO: 27, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO: 28; g) a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO: 31, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO: 32, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO: 33; h) a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO: 36, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO: 37, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO: 38; i) a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO: 41, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO: 42, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO: 43; j) a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO: 46, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO: 47, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO: 48; k) a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO: 51, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO: 52, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO: 53; l) a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO: 56, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO: 57, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO: 58; m) a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO: 61, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO: 62, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO: 63; n) a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO: 66, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO: 67, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO: 68; o) a heavy chain variable region CDR1 comprising the amino acid sequence set forth in SEQ ID NO: 71, a heavy chain variable region CDR2 comprising the amino acid sequence set forth in SEQ ID NO: 72, and a heavy chain variable region CDR3 comprising the amino acid sequence set forth in SEQ ID NO: 73; or p) An antibody comprising a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO: 76, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO: 77, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO:

78.

2. The antibody of claim 1, wherein the single domain antibody comprises a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO: 6, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO: 7, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO:

8.

3. The antibody of claim 1, wherein the single domain antibody comprises a heavy chain variable region CDR1 comprising the amino acid sequence shown in SEQ ID NO: 11, a heavy chain variable region CDR2 comprising the amino acid sequence shown in SEQ ID NO: 12, and a heavy chain variable region CDR3 comprising the amino acid sequence shown in SEQ ID NO:

13.

4. The antibody of any one of claims 1 to 3, wherein the single domain antibody comprises a heavy chain variable region comprising an amino acid sequence having at least about 90% sequence identity to an amino acid sequence selected from SEQ ID NOs: 4, 9, 14, 19, 24, 29, 34, 39, 44, 49, 54, 59, 64, 69, 74 and 79.

5. The antibody of any one of claims 1 to 3, wherein the single domain antibody comprises an Fc region.

6. The antibody of claim 5 , wherein the Fc region comprises an Fc region selected from an IgG1, IgG2, IgG3, and IgG4 Fc region.

7. The antibody of claim 6 , wherein the Fc region comprises an IgG1 Fc region.

8. The antibody of claim 7 , wherein the IgG1 Fc region comprises one or more mutations that enhance covalent binding to FcγRIIa, FcγRIIb, or a combination thereof.

9. The antibody of claim 7 or 8, wherein the IgG1 Fc region comprises the mutations S267E and L328F, or the IgG1 Fc region comprises the mutations N325S and L328F.

10. The antibody of any one of claims 1 to 3, which is an agonist antibody.

11. An immunoconjugate comprising the antibody of any one of claims 1 to 10 linked to a therapeutic agent or label.

12. The immunoconjugate of claim 11 , wherein the therapeutic agent is a cytotoxin or a radioisotope.

13. The immunoconjugate of claim 11 , wherein the label is selected from a radioisotope, a fluorescent dye, and an enzyme.

14. An antigen-recognizing receptor comprising an extracellular antigen-binding domain comprising the antibody of any one of claims 1 to 10.

15. The antigen recognition receptor of claim 14, which is a chimeric antigen receptor (CAR) or a recombinant T cell receptor.

16. The antigen-recognizing receptor according to claim 14 or 15, wherein the antibody contained in the extracellular antigen-binding domain comprises a VHH.

17. An immunoresponsive cell comprising the antigen-recognizing receptor according to any one of claims 14 to 16.

18. The immunoresponsive cell of claim 17, wherein the immunoresponsive cell is selected from T cells, natural killer (NK) cells, cytotoxic T lymphocytes (CTLs), regulatory T cells, natural killer T (NKT) cells, and myeloid cells.

19. The immunoresponsive cell of claim 18 , wherein the immunoresponsive cell is a T cell.

20. 20. A pharmaceutical composition comprising: a) an antibody according to any one of claims 1 to 10, an immunoconjugate according to any one of claims 11 to 13, or an immunoresponsive cell according to any one of claims 17 to 19; and b) a pharmaceutically acceptable carrier agent.

21. One or more nucleic acids encoding the antibody of any one of claims 1 to 10.

22. 22. One or more vectors comprising the nucleic acid of claim 21.

23. 23. A host cell comprising the nucleic acid of claim 21 or the vector of claim 22.

24. 24. A method for preparing an antibody according to any one of claims 1 to 10, comprising expressing said antibody in a host cell according to claim 23 and isolating said antibody from the host cell.

25. 21. The pharmaceutical composition of claim 20, which is used to treat cancer.

26. 26. The pharmaceutical composition of claim 25, wherein the cancer exhibits high microsatellite instability (MSI).

27. 26. The pharmaceutical composition of claim 25, wherein the cancer is selected from mesothelioma, lung cancer, pancreatic cancer, ovarian cancer, breast cancer, colon cancer, pleural tumor, glioblastoma, esophageal cancer, gastric cancer, synovial sarcoma, thymic cancer, endometrial cancer, gastric tumor, bile duct cancer, head and neck cancer, blood cancer, and combinations thereof.

28. 20. A kit comprising an antibody according to any one of claims 1 to 10, an immunoconjugate according to any one of claims 11 to 13, a pharmaceutical composition according to claim 20, one or more nucleic acids according to claim 21, one or more vectors according to claim 22, or an immunoresponsive cell according to any one of claims 17 to 19.

29. 29. The kit of claim 28, further comprising a manual for treating and / or preventing tumors.

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