Emulsifier Mixture

Abstract

The present invention relates to a particular combination and its ability to emulsify lipids contained in feed products.

Description

[Technical field]

[0001] The present invention relates to certain combinations comprising lecithin and their use in feed products. [Background technology]

[0002] Lecithin is a generic term used to describe a multi-component blend containing phospholipids, triglycerides, glycolipids and optionally sterol(s) and / or protein(s).

[0003] Lecithin can be obtained from plants, especially seeds, and from animals, especially from egg yolks or from milk.

[0004] Phospholipids have been linked to reduced mortality as reduced mortality has been observed when animals were fed phospholipids on a regular basis.

[0005] Phospholipids provide highly bioavailable nutrients such as choline, inositol, phosphorus, and essential fatty acids.

[0006] Phospholipids facilitate the absorption of vitamins (A, D, E, K), carotenoids, and pigments.

[0007] Phospholipids also facilitate lipid digestion by helping bile acids form emulsion globules in the small intestine and facilitating lipid absorption at the intestinal mucosa. Improved lipid absorption results in faster growth in animals, especially during the first stages of their life.

[0008] Lipids represent a significant portion of the cost of a feed product. Improving lipid absorption and utilization by animals can reduce the percentage of lipid in the diet, which provides economic benefits.

[0009] The lipids can be of vegetable origin (soybean oil, sunflower oil, palm oil, etc.) or animal origin (tallow, chicken fat, lard, etc.) The lipids are primarily triglycerides and contain saturated and unsaturated acyl chains. Summary of the Invention [Problem to be solved by the invention]

[0010] Therefore, for all these reasons, it is advantageous to feed animals with lecithin containing phospholipids. However, it is necessary to improve the emulsifying properties of lecithin so that the amount of lipids in the feed can be reduced while improving the absorption of lipids during digestion in order to keep the total calories available to the animal for growth the same. In fact, lecithin cannot emulsify lipids efficiently when used alone. [Means for solving the problem]

[0011] The present inventors have surprisingly found that certain combinations rich in phospholipids can facilitate lipid emulsification.

[0012] That is, the present invention relates to a combination comprising or consisting of: - 2.4-15% by weight of deoiled lecithin; - Hydrolyzed lecithin; - Sorbitan monostearate; and - 0-40% by weight of an anticaking agent; Weight percentages are based on the weight of the combination; The mass ratio of sorbitan monostearate / hydrolyzed lecithin is 60 / 40-80 / 20. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0013] In this application, all ranges of values ​​used should be understood to be inclusive, unless otherwise indicated.

[0014] Deoiled lecithin contains no lecithin or has limited oil (e.g. triglyceride) content and is therefore rich in phospholipids.

[0015] Preferably, the deoiled lecithin comprises at least 92% by weight acetone insolubles, more preferably at least 94%, and even more preferably at least 97% by weight acetone insolubles, based on the weight of the deoiled lecithin.

[0016] Preferably, the deoiled lecithin is in powder form.

[0017] Hydrolyzed lecithin is lecithin in which the ester bonds of the phospholipids have been partially hydrolyzed to form alcohol functional groups.

[0018] Preferably, the lecithin of the deoiled lecithin and the hydrolyzed lecithin are each independently obtained from seeds, in particular sunflower seeds, soybean seeds or rapeseed seeds.

[0019] Sorbitan monostearate (CAS number 1338-41-6) is a nonionic surfactant.

[0020] The anti-caking agent is preferably a silica-based product, calcium carbonate or limestone.

[0021] Advantageously, in the combination according to the invention, the weight ratio sorbitan monostearate / hydrolyzed lecithin is between 60 / 40 and 75 / 25.

[0022] Preferably, the mass ratio sorbitan monostearate / hydrolyzed lecithin is 60 / 40 to 70 / 30, in particular the mass ratio sorbitan monostearate / hydrolyzed lecithin is 70 / 30.

[0023] Preferably, the amount of deoiled lecithin is at least 3%, more preferably at least 3.5%, even more preferably at least 4%, by weight based on the weight of the combination.

[0024] Preferably, the amount of deoiled lecithin is at most 10% by weight, more preferably at most 9.5% by weight, based on the weight of the combination.

[0025] Preferably, the amount of anti-caking agent is 0 to 40% by weight, more preferably 20 to 40% by weight, based on the weight of the combination.

[0026] Preferably, the amount of hydrolyzed lecithin is at least 13% by weight, more preferably at least 17% by weight, based on the weight of the combination.

[0027] Preferably, the amount of hydrolyzed lecithin is at most 39% by weight, more preferably at most 33% by weight, based on the weight of the combination.

[0028] Preferably, the amount of sorbitan monostearate is at least 39% by weight, more preferably at least 52% by weight, based on the weight of the combination.

[0029] Preferably, the amount of sorbitan monostearate is at most 78% by weight, more preferably at most 65% by weight, based on the weight of said combination.

[0030] In a first embodiment, the combination according to the invention does not comprise an anti-caking agent.

[0031] Preferably, the combination according to the invention comprises or consists of: - 4-10% by weight of deoiled lecithin; - Hydrolyzed lecithin; - Sorbitan Monostearate; wherein the amount of sorbitan monostearate and hydrolyzed lecithin is 90 to 96% by mass; The mass ratio of sorbitan monostearate / hydrolyzed lecithin is 60 / 40-75 / 25; The weight percentages are based on the weight of the above combination.

[0032] In a second embodiment, the combination according to the invention comprises an anti-caking agent.

[0033] Preferably, the combination according to the invention comprises or consists of: - 3-8% by weight of deoiled lecithin; - Hydrolyzed lecithin; - Sorbitan Monostearate; - 20-40% by weight of anti-caking agent; wherein the amount of sorbitan monostearate and hydrolyzed lecithin is 52 to 77% by mass; The mass ratio of sorbitan monostearate / hydrolyzed lecithin is 60 / 40-75 / 25; The weight percentages are based on the weight of the above combination.

[0034] Preferably, in the combination according to the invention, the mass ratio (sorbitan monostearate+hydrolyzed lecithin) / deoiled lecithin is 7-32, more preferably 9.5-24.

[0035] Preferably, in the combination according to the invention, the hydrolyzed lecithin is sunflower hydrolyzed lecithin or soybean hydrolyzed lecithin.

[0036] Advantageously, in the combination according to the invention, the hydrolyzed lecithin is sunflower hydrolyzed lecithin.

[0037] Advantageously, the combination according to the invention is in powder form.

[0038] The present invention also relates to a method for preparing a combination according to the invention, comprising the steps of: i) mixing and heating sorbitan monostearate and hydrolyzed lecithin until a molten mixture is formed; ii) cooling the molten mixture obtained in step i) to form a cooled mixture; iii) blending the cooled mixture obtained in step ii) and, optionally, an anti-caking agent with the de-oiled lecithin.

[0039] In step i), the heating temperature is preferably at least 50° C., more preferably at least 54° C. Preferably, the temperature is between 50 and 65° C., more preferably between 54 and 60° C.

[0040] In step ii) the molten mixture is preferably cooled by spreading it on an aluminium foil to form a film of the cooled mixture which can be broken into flakes or by spraying the molten mixture in a spray solidification tower to form beads of the cooled mixture.

[0041] The cooled mixture, particularly the flakes, can then be ground to form a powder.

[0042] Preferably, the deoiled lecithin is in powder form.

[0043] This particular combination can readily form an oil-in-water emulsion, especially when the oil is composed of unsaturated triglycerides.

[0044] The present invention also relates to a method for improving the emulsification of one or more lipids by adding to said lipid(s) a combination according to the invention.

[0045] The one or more lipids mentioned above can be saturated and / or unsaturated.

[0046] The lipid(s) are preferably triglycerides, more preferably unsaturated triglycerides, such as triglycerides containing one or more unsaturated acyl chains. In particular, the unsaturated triglycerides contain at least 30% by weight of unsaturated acyl chains. Preferably, the unsaturated acyl chains are C18 acyl chains, such as acyl chains containing 18 carbon atoms.

[0047] Preferably, the one or more lipids (one or more) are a mixture of unsaturated triglycerides. In particular, the mixture of unsaturated triglycerides comprises at least 30% by weight, preferably at least 50% by weight, more preferably at least 70% by weight of unsaturated acyl chains. More particularly, the mixture of unsaturated triglycerides comprises at least 70% by weight of C18 acyl chains containing one or two double bonds.

[0048] As shown in examples 2.2 and 2.3, the combination according to the invention makes it possible to emulsify and re-emulsify unsaturated lipids such as sunflower oil and soybean oil in water.

[0049] These properties are of particular interest in the field of animal nutrition, since during digestion the animal is either in a stationary or active state: in either case, in the presence of the combination according to the invention, lipids can be easily emulsified and re-emulsified in the stomach and intestine with limited kinetic energy.

[0050] The present invention also relates to an oil-in-water emulsion comprising: - One or more lipids - Water; and - A combination according to the present invention.

[0051] Preferably the oil-in-water emulsion is free of sorbitan monostearate and hydrolyzed lecithin other than that present in the combination.

[0052] Preferably, the oil-in-water emulsion according to the present invention does not contain any deoiled lecithin other than that present in the combination.

[0053] Advantageously, the oil-in-water emulsion according to the invention is lecithin-free.

[0054] The one or more lipids are as described above, including optional features.

[0055] The amount of water is preferably at least 70% by weight, more preferably at least 80% by weight, based on the weight of the oil-in-water emulsion.

[0056] The total amount of lipid(s) is preferably at least 2.5% by weight, more preferably at least 10% by weight, based on the weight of the oil-in-water emulsion.

[0057] The total amount of lipid(s) is preferably at most 20% by weight, more preferably at most 15% by weight, even more preferably at most 12.5% ​​by weight, based on the weight of the oil-in-water emulsion.

[0058] By "total amount of lipid" is meant the amount of all lipid molecules.

[0059] The above combinations, including their optional and advantageous features, are as described above.

[0060] The amount of the combination according to the invention is preferably at least 0.01% by weight, more preferably at least 0.1% by weight, even more preferably at least 0.25% by weight, based on the weight of the oil-in-water emulsion.

[0061] The amount of the combination according to the invention is preferably at most 2.5% by weight, more preferably at most 2% by weight, even more preferably at most 1.5% by weight, based on the weight of the oil-in-water emulsion.

[0062] Preferably, the amount of the combination according to the present invention is 0.01 to 2.5% by mass; more preferably 0.1 to 2.5% by mass, for example 0.1 to 2.0% by mass, 0.1 to 1.5% by mass; even more preferably 0.25 to 2.5% by mass, for example 0.25 to 2.0% by mass, 0.25 to 1.5% by mass, the mass % being based on the weight of the oil-in-water emulsion.

[0063] The present invention also relates to a feed product comprising the combination according to the invention.

[0064] This combination, including its preferred and advantageous features, is as described above.

[0065] Preferably the feed product contains no sorbitan monostearate and no hydrolyzed lecithin other than those present in the above combination.

[0066] Preferably the feed product according to the invention does not contain any de-oiled lecithin other than that present in the combination.

[0067] Advantageously, the feed product according to the invention is lecithin-free.

[0068] Preferably, the feed product comprises at least 100 g, more preferably at least 125 g of the combination according to the invention per tonne of feed product.

[0069] The feed product can be a grain meal, pellets, crumbs or flakes.

[0070] The present invention also relates to a method for increasing the weight of an animal by feeding the animal a combination according to the present invention.

[0071] The animals are preferably small animals, such as chickens, birds, fish, ducks, rabbits, piglets, shrimp, and the like.

[0072] Preferably, the combination is incorporated into a feed product.

[0073] As shown in Example 3, the more the feed product contains the combination according to the invention, the greater the weight gain of the animals after feeding for at least 3 weeks, in particular at least 125 g of the combination per tonne of feed product.

[0074] The invention is further described in the following examples, it being understood that the invention as claimed is in no way intended to be limited by these examples. EXAMPLES

[0075] Example 1: Preparation of the Powder Combination Mixtures 1 and 2 were prepared according to the following method: Sorbitan monostearate (RADIAMULS SORB 2145K from Oleon) and hydrolyzed lecithin (LECICO SUN FM 580 from Lecico; 56% acetone insolubles, hydrolyzed sunflower lecithin containing mainly phospholipids and glycolipids) were heated at 57° C. under stirring until the sorbitan monostearate was melted. The resulting mixture was then cooled on aluminum foil to form a film, which was then broken to form flakes, which were then crushed to form a powder.

[0076] Mixture 3 was prepared according to the method described above using hydrolyzed soybean lecithin instead of hydrolyzed sunflower lecithin (LECICO F 580 IP from Lecico; hydrolyzed soybean lecithin containing 56% acetone insolubles, mainly phospholipids and glycolipids).

[0077] The contents of Mixes 1, 2 and 3 are listed in Table 1 below. Comparative Mixes 1 and 2 were prepared according to the same method as above and the amounts listed in Table 1 below.

[0078] [Table 1]

[0079] Combinations 1 to 3 according to the invention were then obtained by mixing deoiled lecithin (Bergapur from Berg+Schmidt; powdered deoiled soy lecithin containing 97% acetone insolubles, mainly phospholipids) with the mixtures 1 or 2 prepared as described above, in the amounts set out in Table 2 below.

[0080] Combination 3-2 according to the invention was prepared similarly to combinations 1-3, with the addition of an anti-caking agent (Sipernat 2200 from Evonik) during mixing of deoiled lecithin with mixture 2, according to the amounts set out in Table 2 below.

[0081] Combination 4 according to the invention was prepared similarly to combinations 1-3 using mixture 3 according to the amounts set out in Table 2 below.

[0082] Comparative Combinations 1 and 2 were prepared by mixing deoiled lecithin (Bergapur from Berg+Schmidt; powdered deoiled soy lecithin containing 97% acetone insolubles, primarily phospholipids) with Comparative Combination 1 or 2 prepared as described above according to the amounts set forth in Table 2 below.

[0083] Comparative Combination 3 was prepared by mixing deoiled lecithin (Bergapur from Berg+Schmidt; powdered deoiled soy lecithin containing 97% acetone insolubles, primarily phospholipids) with Mixture 2 prepared as above according to the amounts set forth in Table 2 below.

[0084] Comparative Combination 4 was prepared similarly to Comparative Combination 3 with the addition of an anti-caking agent (Sipernat 2200 from Evonik) according to the amounts set out in Table 2 below.

[0085] Comparative Combination 5 was prepared by mixing lecithin (Lecico F600 IP from Lecico; a flowable soy lecithin containing 62% acetone insolubles), an anti-caking agent (Sipernat 2200 from Evonik) and Mixture 2 prepared above according to the amounts set forth in Table 2 below.

[0086] [Table 2]

[0087] Example 2: Oil-in-water emulsion 2.1. Chemicals Used Combinations 1 to 4 according to the invention as prepared in Example 1; - Comparative combinations 1 to 5 prepared in Example 1; - oil ○ Refined soybean oil: Radia 6119 Phytorob ST from Oleon; o Refined sunflower oil: Radia 6121 Phytorob SFO from Oleon.

[0088] 2.2. Preparation of oil-in-water emulsions 1 to 6 Emulsions 1-6 were prepared according to the methods and amounts set out in Table 3 below. The combination according to the invention and the oil were placed in a glass bottle, closed and heated to 80° C. for 1 hour. Tap water heated to 50°C was placed in a measuring cylinder. The combination and oil were added to the top of the cylinder after reaching 45°C. The cylinder was then inverted once and observed for emulsion formation.

[0089] [Table 3]

[0090] 2.3. Properties of oil-in-water emulsions 1 to 6 To test the stability of each emulsion, each cylinder was inverted 10 times. Each emulsion was quite stable as no creaming or oiling out was observed immediately after inversion, and they appeared to start dephasing after at least 1 minute. To test the re-emulsification ability, each emulsion was left to stand for 1 hour until the complete separation of the two clear phases (water and oil) was observed, after which each cylinder was inverted one more time. It was observed that an emulsion was again formed in each cylinder containing the combination according to the invention.

[0091] 2.4.Comparative Example Attempts to form emulsions were made according to the method described in Example 2.2 using the following: A: 12.5 wt% soybean oil and 0.25 wt% comparative combination 1 instead of the combination according to the invention; - B: 12.5 wt% soybean oil and 0.25 wt% comparative combination 2 instead of the combination according to the invention; - C: 12.5 wt% soybean oil and 0.25 wt% deoiled lecithin (Bergapur from Berg+Schmidt) instead of the combination according to the invention; - D: 12.5 wt% soybean oil and 0.25 wt% comparative combination 3 instead of the combination according to the invention; E: 12.5 wt% soybean oil and 0.25 wt% comparative combination 4 instead of the combination according to the invention; F: 12.5 wt% soybean oil and 0.25 wt% comparative combination 5 instead of the combination according to the invention.

[0092] In all cases A to F, no emulsion was formed.

[0093] It can be observed that while it was possible to form an emulsion using combination 3 / 2 according to the invention (emulsion 5), it was not possible to form an emulsion using comparative combination 5 (attempt F). Comparative combination 5 contains lecithin instead of deoiled lecithin. This shows the importance of using deoiled lecithin.

[0094] Thus, only the combinations according to the invention with specific mass ratios allow the formation of oil-in-water emulsions. These emulsions are stable for at least 1 minute at temperatures between 35 and 45°C and can be easily re-emulsified. Moreover, high-speed stirring is not required for emulsification and re-emulsification.

[0095] Example 3: Evaluation of the contribution of the combination according to the invention to the weight gain of chickens 3.1. Method Four treatment units were created containing 375 chickens each, with ample floor space and numerous water and feed stations.

[0096] Combination 3:2 was tested at three application rates, 125g / tonne, 250g / tonne and 500g / tonne, and incorporated into Gold Coin (Malaysia; containing triglycerides with unsaturated C18 acyl chains and no lecithin or sorbitan fatty acid esters) pelleted commercial broiler feed (starter / grower / finisher).

[0097] Combination 3-2 was prepared as combination 3 with the addition of 20 wt % of an anti-caking agent (silica) based on the weight of combination 3-2 during mixing of the deoiled lecithin and mixture 2.

[0098] In the fourth treatment unit, the "control," chickens were fed Gold Coin (Malaysia) pelleted commercial broiler feed. During the first and second weeks, 20 chickens per treatment unit were weighed. During the third and fourth weeks, 40 chickens per treatment unit were weighed. Week 5 (capture phase): All chickens were weighed. Chickens were weighed and mortality recorded weekly.

[0099] 3.2.Results

[0100] [Table 4]

[0101] Mortality rate after 5 weeks: Control unit: 16 birds (4.2%) 125g / ton processing unit: 3 birds (0.80%) 250g / tonne processing unit: 5 birds (1.33%). 500g / ton processing unit: 0 birds

[0102] It can be observed that chickens fed with a feed product comprising the combination according to the invention gained more weight after 5 weeks than chickens fed with a feed product not comprising the combination according to the invention.

[0103] With 500 g of combination 2 per tonne of feed product, the average weight of the chickens fed with it is greater after the first week than the average weight of chickens fed without the combination according to the invention (control).

[0104] After 5 weeks, with only 125 g of combination 2 per tonne of feed, the average weight of the chickens is about 27% greater than the average weight of chickens fed without the combination according to the invention.

[0105] Furthermore, the mortality rate of chickens fed with the combination according to the invention is lower than that of chickens fed without the combination according to the invention, in particular by using at least 125 g of the formulation according to the invention per ton of feed product, the mortality rate is at least three times lower.

Claims

1. Combinations including or consisting of: - 2.4 to 15% by weight of deoiled lecithin; - at least 13% by weight of hydrolyzed lecithin; sorbitan monostearate; and - 0 to 40% by weight of an anti-caking agent; The weight percentages are based on the weight of the combination; The mass ratio of sorbitan monostearate / hydrolyzed lecithin is 60 / 40 to 80 / 20.

2. 2. The combination according to claim 1, wherein the mass ratio sorbitan monostearate / hydrolyzed lecithin is from 60 / 40 to 75 / 25.

3. 2. The combination of claim 1, wherein the hydrolyzed lecithin is sunflower or soybean hydrolyzed lecithin.

4. 2. The combination according to claim 1, wherein the mass ratio (sorbitan monostearate+hydrolyzed lecithin) / deoiled lecithin is between 7 and 32.

5. The combination of claim 1 , wherein the combination is in powder form.

6. A method for producing the combination according to any one of claims 1 to 5, comprising the steps of: i) mixing and heating sorbitan monostearate and hydrolyzed lecithin until a molten mixture is formed; ii) cooling the molten mixture obtained in step i) to form a cooled mixture; iii) blending the cooled mixture from step ii) and optionally an anti-caking agent with de-oiled lecithin.

7. 6. A method for improving the emulsification of one or more lipids by adding to said one or more lipids a combination according to any one of claims 1 to 5.

8. An oil-in-water emulsion comprising: - one or more lipids - water; and a combination according to any one of claims 1 to 5; The oil-in-water emulsion is free of sorbitan monostearate and hydrolyzed lecithin other than that present in the combination.

9. 6. A feed product comprising the combination of any one of claims 1 to 5, wherein the feed product does not contain any sorbitan monostearate and hydrolyzed lecithin other than the sorbitan monostearate and hydrolyzed lecithin present in the combination.

10. A method for increasing the weight of an animal by feeding the animal a combination according to any one of claims 1 to 5.